##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5876088
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8246326467541	31.0	31.0	34.0	30.0	34.0
2	31.792285615872327	31.0	31.0	34.0	30.0	34.0
3	31.992099335476254	33.0	31.0	34.0	30.0	34.0
4	35.740661303915125	37.0	35.0	37.0	35.0	37.0
5	35.63629901390176	37.0	35.0	37.0	33.0	37.0
6	35.67786067873728	37.0	35.0	37.0	33.0	37.0
7	35.6053629557624	37.0	35.0	37.0	33.0	37.0
8	35.59492642043482	37.0	35.0	37.0	33.0	37.0
9	37.18532789161769	39.0	37.0	39.0	33.0	39.0
10-11	37.12573075828681	39.0	37.0	39.0	33.0	39.0
12-13	37.05164260644156	39.0	37.0	39.0	33.0	39.0
14-15	38.25457710980503	40.0	38.0	41.0	33.0	41.0
16-17	38.25812283614541	40.0	37.5	41.0	33.0	41.0
18-19	38.31428001078268	40.0	38.0	41.0	33.0	41.0
20-21	38.32803031540712	40.0	38.0	41.0	33.0	41.0
22-23	38.28877622322879	40.0	38.0	41.0	33.0	41.0
24-25	38.215084253333174	40.0	37.0	41.0	33.0	41.0
26-27	38.07406577301089	40.0	37.0	41.0	33.0	41.0
28-29	38.012070445507284	40.0	37.0	41.0	33.0	41.0
30-31	37.88721390830089	40.0	37.0	41.0	33.0	41.0
32-33	37.76074805210541	40.0	36.5	41.0	33.0	41.0
34-35	37.66211950876161	40.0	36.0	41.0	32.0	41.0
36-37	37.581447299632	40.0	36.0	41.0	32.0	41.0
38-39	37.462558167951194	40.0	36.0	41.0	32.0	41.0
40-41	37.33628708419616	40.0	36.0	41.0	31.0	41.0
42-43	37.20892105427965	39.0	35.5	41.0	31.0	41.0
44-45	36.998207225623574	39.0	35.0	41.0	31.0	41.0
46-47	36.75520839715131	39.0	35.0	41.0	30.0	41.0
48-49	36.48948841474124	39.0	35.0	41.0	30.0	41.0
50-51	36.279906036124714	39.0	35.0	41.0	29.5	41.0
52-53	36.083750107214186	38.0	35.0	40.5	29.0	41.0
54-55	35.87747366955702	38.0	35.0	40.0	29.0	41.0
56-57	35.60808883393169	37.5	34.0	40.0	28.0	41.0
58-59	35.32305932109934	37.0	34.0	40.0	28.0	41.0
60-61	35.06803514515099	36.5	34.0	40.0	28.0	41.0
62-63	34.79264401758449	36.0	34.0	39.5	27.5	41.0
64-65	34.52032091759007	35.5	33.5	39.0	27.0	41.0
66-67	34.297658918654726	35.0	33.0	39.0	27.0	41.0
68-69	34.11061781239491	35.0	33.0	38.5	26.0	41.0
70-71	33.92563709052689	35.0	33.0	38.0	26.0	40.5
72-73	33.73357631812185	35.0	33.0	37.0	26.0	40.0
74-75	33.55733712633303	35.0	33.0	37.0	26.5	39.5
76-77	33.3994179460893	35.0	33.0	36.0	27.0	39.0
78-79	33.28688329718683	35.0	33.0	36.0	27.0	39.0
80-81	33.14631443572662	35.0	33.0	36.0	27.0	38.0
82-83	32.954316545293395	35.0	33.0	35.0	27.0	37.0
84-85	32.812110029665995	35.0	33.0	35.0	27.0	37.0
86-87	32.67692868112255	35.0	33.0	35.0	26.0	36.5
88-89	32.56798502677292	35.0	33.0	35.0	26.0	36.0
90-91	32.47371559105309	35.0	33.0	35.0	26.0	36.0
92-93	32.39283882406117	35.0	33.0	35.0	26.0	36.0
94-95	32.34707980887965	35.0	33.0	35.0	26.0	35.5
96-97	32.29060873492705	35.0	33.0	35.0	26.0	35.0
98-99	32.25553863386661	35.0	33.0	35.0	26.0	35.0
100	31.76316607239374	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.492064159263105
1101	2	0.5926902359474191
1101	3	0.4793672939418556
1101	4	0.37268804110291853
1101	5	0.3823966249826327
1101	6	0.3661398661568711
1101	7	0.39755993460565975
1101	8	0.40468484134055416
1101	9	0.4642622704206687
1101	10-11	0.4994819709397973
1101	12-13	0.4906116711782431
1101	14-15	0.6147141394967335
1101	16-17	0.6453790909353501
1101	18-19	0.6461263587912569
1101	20-21	0.6355447184592578
1101	22-23	0.6503763889834175
1101	24-25	0.6802686825166546
1101	26-27	0.6676918425208029
1101	28-29	0.7046559410168953
1101	30-31	0.7703642869922973
1101	32-33	0.7416918391558696
1101	34-35	0.7546788660471933
1101	36-37	0.7934548827026546
1101	38-39	0.7792728936737632
1101	40-41	0.8020710431737044
1101	42-43	0.7847722291088388
1101	44-45	0.7107209164669968
1101	46-47	0.7410478660281328
1101	48-49	0.7196362175537558
1101	50-51	0.6738604586253985
1101	52-53	0.7088472266532264
1101	54-55	0.6320871333900939
1101	56-57	0.7035192976121394
1101	58-59	0.6626360962737436
1101	60-61	0.7026387659274036
1101	62-63	0.6915492795995135
1101	64-65	0.664322302077629
1101	66-67	0.6444288791981521
1101	68-69	0.7314248067743421
1101	70-71	0.5991463615539985
1101	72-73	0.5814556031723015
1101	74-75	0.5697266620336023
1101	76-77	0.5842039540340025
1101	78-79	0.5523019532303906
1101	80-81	0.4894066044655787
1101	82-83	0.4963666887722553
1101	84-85	0.565318860794406
1101	86-87	0.4975375867915304
1101	88-89	0.5389964357567294
1101	90-91	0.5293011200399604
1101	92-93	0.5402560084916104
1101	94-95	0.5418250860137874
1101	96-97	0.5724657190933016
1101	98-99	0.551120448045701
1101	100	0.5944427236466083
1102	1	0.43068173522246767
1102	2	0.5304909645255087
1102	3	0.40752182113386937
1102	4	0.328916052995325
1102	5	0.2502003198377878
1102	6	0.3329707586284698
1102	7	0.3242664944896063
1102	8	0.34965617301615026
1102	9	0.44769864755561883
1102	10-11	0.4561450469569195
1102	12-13	0.5253806062384712
1102	14-15	0.6325963054200869
1102	16-17	0.606117732921561
1102	18-19	0.6000944672911857
1102	20-21	0.6037672262868696
1102	22-23	0.5791091025590163
1102	24-25	0.611422945741694
1102	26-27	0.6682963890411457
1102	28-29	0.6744122785397408
1102	30-31	0.6799278006632505
1102	32-33	0.7053440074629762
1102	34-35	0.6787558773755862
1102	36-37	0.6686604259603328
1102	38-39	0.7003513937466437
1102	40-41	0.6440267837363294
1102	42-43	0.7127985446417497
1102	44-45	0.7103573464440984
1102	46-47	0.6916583597268016
1102	48-49	0.635005127850313
1102	50-51	0.5933884452179257
1102	52-53	0.6125239234148978
1102	54-55	0.5825048800139783
1102	56-57	0.670031936828515
1102	58-59	0.6531163882022071
1102	60-61	0.6393993110789182
1102	62-63	0.5893595297563508
1102	64-65	0.637479663928822
1102	66-67	0.6072999197544959
1102	68-69	0.5531201864504993
1102	70-71	0.5322095654354584
1102	72-73	0.582303137018819
1102	74-75	0.5101412668899172
1102	76-77	0.5256930421804498
1102	78-79	0.47908788600874885
1102	80-81	0.4636754488557102
1102	82-83	0.4639810854285713
1102	84-85	0.4675406437487126
1102	86-87	0.48724166226609356
1102	88-89	0.43082677982049944
1102	90-91	0.5269634585094849
1102	92-93	0.518130043354752
1102	94-95	0.4747929461458966
1102	96-97	0.4932562502104503
1102	98-99	0.4985573116793347
1102	100	0.5788189499474683
1103	1	0.3274024141392182
1103	2	-0.12811965021749216
1103	3	0.20727158271299828
1103	4	0.23092659615011968
1103	5	0.234222698191779
1103	6	0.27826095766192793
1103	7	0.3471792037163439
1103	8	0.33518789853781783
1103	9	0.40828410200020926
1103	10-11	0.4085624904265046
1103	12-13	0.4667831899284067
1103	14-15	0.5657241666291384
1103	16-17	0.5637402358629515
1103	18-19	0.5441449261573155
1103	20-21	0.549737017708722
1103	22-23	0.5536702531529585
1103	24-25	0.5379973136234781
1103	26-27	0.5857000155523195
1103	28-29	0.5928255730843475
1103	30-31	0.6545759738919585
1103	32-33	0.6639152505069816
1103	34-35	0.6452025842608151
1103	36-37	0.6597710215160504
1103	38-39	0.6803170065107764
1103	40-41	0.6900280423011864
1103	42-43	0.6898316396519135
1103	44-45	0.7233170170730858
1103	46-47	0.6475395034870459
1103	48-49	0.6457494318411676
1103	50-51	0.6796705898835
1103	52-53	0.6765016108113429
1103	54-55	0.6753896978819469
1103	56-57	0.6481027707150488
1103	58-59	0.6699081488991467
1103	60-61	0.6682306835122844
1103	62-63	0.6927643084713964
1103	64-65	0.6855419266901635
1103	66-67	0.6725945557336814
1103	68-69	0.5984143075457524
1103	70-71	0.5424223001223609
1103	72-73	0.5286639259732766
1103	74-75	0.5784375673582858
1103	76-77	0.5533602270145295
1103	78-79	0.5126968494368285
1103	80-81	0.4496806500293786
1103	82-83	0.48008491406795173
1103	84-85	0.49029439767251404
1103	86-87	0.5293814606237675
1103	88-89	0.548813476114347
1103	90-91	0.4890753262273364
1103	92-93	0.5321778776423756
1103	94-95	0.4993634003055476
1103	96-97	0.4937840666632738
1103	98-99	0.5039698899188778
1103	100	0.4318420105226579
1104	1	0.33337976731847974
1104	2	0.40464818703076944
1104	3	0.3621076185090928
1104	4	0.2775738035917499
1104	5	0.2566973505541128
1104	6	0.29470173800640254
1104	7	0.27824053221388567
1104	8	0.2659005610200822
1104	9	0.3435008114352627
1104	10-11	0.30868417349431354
1104	12-13	0.35783074901048906
1104	14-15	0.42670277928760214
1104	16-17	0.4076833025131208
1104	18-19	0.42820469234249003
1104	20-21	0.41380563894924194
1104	22-23	0.3910657393669652
1104	24-25	0.4242440094211446
1104	26-27	0.47199302722035696
1104	28-29	0.49381729921058337
1104	30-31	0.48185593567143314
1104	32-33	0.5190648615019668
1104	34-35	0.4598487191444818
1104	36-37	0.4693278609878391
1104	38-39	0.48739936082727553
1104	40-41	0.49918087762495844
1104	42-43	0.48386467191360794
1104	44-45	0.5298617447330898
1104	46-47	0.47336875941969225
1104	48-49	0.46071334356688
1104	50-51	0.4312693162906953
1104	52-53	0.4412247414316752
1104	54-55	0.46205328898876985
1104	56-57	0.4525961531509566
1104	58-59	0.3499604539297536
1104	60-61	0.31110765387927586
1104	62-63	0.3799550895105739
1104	64-65	0.37107549165890674
1104	66-67	0.3396619885520238
1104	68-69	0.3528437881536064
1104	70-71	0.3941174997385204
1104	72-73	0.3691744898975884
1104	74-75	0.3649300038222023
1104	76-77	0.3310720343437552
1104	78-79	0.3515385572007048
1104	80-81	0.3356652529810802
1104	82-83	0.29505439538590394
1104	84-85	0.31582628269957524
1104	86-87	0.3278303029470564
1104	88-89	0.32851623973766664
1104	90-91	0.33496151865837476
1104	92-93	0.2701364341276289
1104	94-95	0.26868544580473497
1104	96-97	0.28039169350546445
1104	98-99	0.23010379658389013
1104	100	0.38997391871779286
1105	1	0.10907247121721042
1105	2	-0.38258814452480294
1105	3	0.018148014262941103
1105	4	0.0814298081896041
1105	5	0.13894078042872593
1105	6	0.180056247029718
1105	7	0.22811560949337206
1105	8	0.17821880957460223
1105	9	0.28594812006697623
1105	10-11	0.2747277033402469
1105	12-13	0.2878071589745872
1105	14-15	0.34629751365775974
1105	16-17	0.35375276926465915
1105	18-19	0.3669117950119727
1105	20-21	0.3682815693291914
1105	22-23	0.35126939912235855
1105	24-25	0.3737769172400647
1105	26-27	0.3866839758499552
1105	28-29	0.4090820264099122
1105	30-31	0.390866033130159
1105	32-33	0.38506037078305866
1105	34-35	0.4464978360792671
1105	36-37	0.435492119967428
1105	38-39	0.4253512212912227
1105	40-41	0.5174625605880223
1105	42-43	0.4428289417840858
1105	44-45	0.4953369592141925
1105	46-47	0.5111622579691044
1105	48-49	0.4882642503671448
1105	50-51	0.5298285454520126
1105	52-53	0.5287836802677504
1105	54-55	0.5220896373542061
1105	56-57	0.4906422790550451
1105	58-59	0.48790970413003976
1105	60-61	0.4734991762637648
1105	62-63	0.4725285105748185
1105	64-65	0.4678352370131833
1105	66-67	0.46683641956929023
1105	68-69	0.4640363146313575
1105	70-71	0.46791758491242774
1105	72-73	0.48561596065390944
1105	74-75	0.4400332498123305
1105	76-77	0.4099430035654308
1105	78-79	0.3259291775348956
1105	80-81	0.3610023898414667
1105	82-83	0.34973377835744657
1105	84-85	0.39498507936210814
1105	86-87	0.4023620136552566
1105	88-89	0.39322675942577234
1105	90-91	0.3021467636251032
1105	92-93	0.3104363421765086
1105	94-95	0.37004031470112864
1105	96-97	0.327443092911615
1105	98-99	0.3162848891005723
1105	100	0.34516668905691006
1106	1	0.2952896562970899
1106	2	0.33566160939529155
1106	3	0.2881730098386228
1106	4	0.1648224785917165
1106	5	0.2061907259474367
1106	6	0.23693944938857925
1106	7	0.24089748982175507
1106	8	0.22597788089169768
1106	9	0.3077284076404183
1106	10-11	0.28344754666815675
1106	12-13	0.29331241623596327
1106	14-15	0.3995408687689803
1106	16-17	0.3613593807333757
1106	18-19	0.39135261224590323
1106	20-21	0.4086654101063516
1106	22-23	0.3687569415556027
1106	24-25	0.3185470208349557
1106	26-27	0.3769471972516669
1106	28-29	0.34344888072622126
1106	30-31	0.3932484961523528
1106	32-33	0.3818770255296542
1106	34-35	0.35775327506680554
1106	36-37	0.4081088452106201
1106	38-39	0.38037272860195515
1106	40-41	0.38654225830097033
1106	42-43	0.36424680082308214
1106	44-45	0.3678670116771059
1106	46-47	0.3590921299490546
1106	48-49	0.4132958933911226
1106	50-51	0.4019740258453339
1106	52-53	0.4257787118668972
1106	54-55	0.44197137274042575
1106	56-57	0.4622046268548061
1106	58-59	0.45342961649195956
1106	60-61	0.406727086478611
1106	62-63	0.44172708718720344
1106	64-65	0.4072629860511583
1106	66-67	0.4095923975193614
1106	68-69	0.3790291260266514
1106	70-71	0.4557771065594167
1106	72-73	0.39012908808453517
1106	74-75	0.39200392206021206
1106	76-77	0.3487543900812753
1106	78-79	0.36084590526447613
1106	80-81	0.3453587281724495
1106	82-83	0.3407206575084558
1106	84-85	0.2841084498597297
1106	86-87	0.3727998676922155
1106	88-89	0.35922727718082115
1106	90-91	0.3216501283016342
1106	92-93	0.32894219470992425
1106	94-95	0.3704281337091757
1106	96-97	0.33552712133840856
1106	98-99	0.38168443072647307
1106	100	0.48642951409811985
1107	1	0.03081273974075671
1107	2	-0.5083104996341916
1107	3	-0.07228214337960281
1107	4	0.030351373043117746
1107	5	0.0937399681423301
1107	6	0.15373930491252707
1107	7	0.13853081955018354
1107	8	0.09944761482780962
1107	9	0.1160121210808569
1107	10-11	0.21513707412624683
1107	12-13	0.16782167424108252
1107	14-15	0.21369510581726558
1107	16-17	0.21406349427586235
1107	18-19	0.19979710545567286
1107	20-21	0.17964147391100482
1107	22-23	0.19702281015075584
1107	24-25	0.24810812233882729
1107	26-27	0.2506500014799684
1107	28-29	0.2014904591223896
1107	30-31	0.31171064871248433
1107	32-33	0.2835625385975433
1107	34-35	0.3094059765320907
1107	36-37	0.28151470006049095
1107	38-39	0.29556280898658827
1107	40-41	0.29381000786136013
1107	42-43	0.27488152246344555
1107	44-45	0.2616716619532582
1107	46-47	0.3735136896860567
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2216	78-79	-0.7297844224425134
2216	80-81	-0.6914993396272706
2216	82-83	-0.5481360808849729
2216	84-85	-0.6163269167977674
2216	86-87	-0.5749206733038434
2216	88-89	-0.5605074812315678
2216	90-91	-0.6075145428649904
2216	92-93	-0.6669311946364722
2216	94-95	-0.5879382540046656
2216	96-97	-0.6294952532927311
2216	98-99	-0.6653372819827119
2216	100	-0.6843003522103288
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	5.0
12	12.0
13	84.0
14	316.0
15	1092.0
16	2701.0
17	5249.0
18	9479.0
19	14818.0
20	21251.0
21	30519.0
22	41830.0
23	48055.0
24	50614.0
25	52739.0
26	58454.0
27	71362.0
28	90488.0
29	111839.0
30	138865.0
31	172259.0
32	215541.0
33	272617.0
34	372708.0
35	512372.0
36	764472.0
37	1291643.0
38	1090370.0
39	433399.0
40	932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.921930821598615	22.015242790923768	13.656455446885966	24.40637094059165
2	14.82722792876891	20.281050851755634	40.28979858929968	24.60192263017578
3	17.720582541620754	26.84281332034258	29.901880113513073	25.53472402452359
4	12.128273908112854	17.454703795386	39.42287363432186	30.99414866217929
5	12.398960365641964	38.92275786294962	35.3505974902279	13.327684281180522
6	29.005607131819673	39.53157270619501	17.880943920513104	13.581876241472218
7	25.62437181610962	32.50046885109868	24.27083017787751	17.60432915491419
8	25.917804498503084	35.199864263435124	19.76074898810229	19.121582249959495
9	25.841546280450533	14.506215699969093	21.871524047972052	37.78071397160832
10-11	25.491236686720825	26.106773758323566	27.451545994546034	20.95044356040958
12-13	25.901807461018283	23.86877119607467	27.973066434675587	22.25635490823146
14-15	23.167294676711762	24.794992213095256	25.817020801132557	26.22069230906042
16-17	21.48891648366666	27.342341242263647	27.220249525584162	23.948492748485535
18-19	20.82665371927718	27.406992543338355	29.901875193155718	21.864478544228746
20-21	22.56165649081459	26.596435092109378	29.014592965775083	21.827315451300947
22-23	23.04641258154307	26.16966608101108	28.694029444339687	22.08989189310617
24-25	22.46974517740374	26.193327941991342	29.55953859098094	21.777388289623982
26-27	22.399900137540627	26.409331932399855	28.428917431717256	22.761850498342262
28-29	21.43465176151208	27.055976697421823	29.102772116414865	22.40659942465123
30-31	22.468068892092834	26.567309747573553	28.899465086295507	22.0651562740381
32-33	22.953927851318767	26.868164670100242	27.987821149036566	22.190086329544418
34-35	22.789594499925162	26.689448917817543	28.883510573529176	21.63744600872812
36-37	22.50708294361827	26.201717877608367	28.5204203885306	22.77077879024276
38-39	22.64201965661508	26.2189487291545	29.280551959058478	21.858479655171944
40-41	22.165350223559628	25.409292041045862	29.78021330956231	22.645144425832203
42-43	22.36111585022531	25.68033281600643	28.793535169394286	23.16501616437398
44-45	22.56963306199635	26.678599775905333	28.47938118013209	22.272385981966234
46-47	22.663700747844484	26.495025261704725	27.656793090913546	23.184480899537245
48-49	23.59526678918087	26.25728305571078	27.62963271181925	22.517817443289108
50-51	22.88920792200525	26.23737935851199	27.420394316763126	23.453018402719632
52-53	22.244169930743038	26.836961937942387	27.31734106092353	23.60152707039105
54-55	22.3443305728978	26.79960766264433	27.13100287939268	23.725058885065184
56-57	23.545268165885336	26.146669473525137	26.884771709317246	23.423290651272286
58-59	22.229164686532705	26.639293521671053	27.887277826842084	23.244263964954158
60-61	22.388295669486542	27.388541156210987	26.986657690163078	23.23650548413939
62-63	22.898448763871475	26.49607187639123	26.514511014811216	24.090968344926083
64-65	22.00481000284543	26.592734826299402	27.11342988736724	24.289025283487923
66-67	22.74057161839646	26.21091617416213	27.062971146790176	23.985541060651236
68-69	22.216851972887312	26.168889368429465	27.507173466551066	24.107085192132157
70-71	22.12227767154592	25.83164909132619	27.616900236826325	24.429173000301564
72-73	22.819901608008593	25.91232466225829	27.893889608188303	23.373884121544812
74-75	22.982255579726086	26.329125449013773	27.349152005867865	23.339466965392276
76-77	22.645044816903194	26.377497741625632	26.782345809362567	24.195111632108603
78-79	22.31957024220676	26.45390937523719	27.00634643589048	24.220173946665568
80-81	21.87918633853105	26.99578710163331	27.666486239899076	23.458540319936567
82-83	22.92286794934316	25.781001038382012	28.701183472988323	22.5949475392865
84-85	23.284840923183932	26.398998388370604	27.04712205571716	23.269038632728307
86-87	21.62964908230356	27.162284553165854	27.594724869773064	23.613341494757524
88-89	22.71893121301492	25.848562924387835	27.40174166176007	24.030764200837172
90-91	22.266438132699808	25.89077274536724	27.639343514565805	24.20344560736714
92-93	22.69670025652994	26.290748966157533	27.209261458429513	23.80328931888301
94-95	21.424260897001925	26.243674665680956	27.961840879486534	24.370223557830585
96-97	21.82831723324659	26.13117217384911	27.437905652450695	24.602604940453602
98-99	21.089488263237985	26.982107861344538	27.328085340260543	24.600318535156934
100	21.994459711124215	26.218691525749367	26.427133653337474	25.359715109788944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	173.0
1	115.0
2	107.0
3	158.5
4	109.5
5	87.0
6	192.5
7	474.5
8	808.5
9	932.0
10	758.5
11	595.5
12	594.5
13	678.0
14	925.0
15	1366.5
16	1883.5
17	2545.0
18	3531.0
19	4634.5
20	5975.5
21	7417.0
22	8773.5
23	10281.5
24	11810.0
25	13564.5
26	16296.0
27	19198.5
28	24500.0
29	36146.0
30	52103.5
31	66596.5
32	86622.0
33	106123.5
34	119600.0
35	135807.5
36	150587.5
37	180479.5
38	194949.5
39	180418.0
40	166306.0
41	153156.5
42	149453.5
43	157314.0
44	172306.5
45	209624.5
46	245284.0
47	267797.5
48	283863.5
49	275553.0
50	260111.0
51	265539.5
52	267289.5
53	254439.5
54	244523.5
55	210826.5
56	181021.5
57	147501.5
58	113100.5
59	112113.5
60	92986.0
61	60262.5
62	43421.0
63	36699.5
64	26585.5
65	11402.5
66	5558.0
67	3646.0
68	2645.5
69	2089.5
70	1602.5
71	1161.5
72	816.0
73	674.5
74	544.5
75	390.5
76	304.0
77	182.5
78	85.5
79	39.0
80	14.0
81	5.0
82	4.0
83	3.5
84	1.5
85	1.0
86	1.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013376246237292567
2	0.004816129370424677
3	0.0019230481231731042
4	0.026105803725199488
5	0.0029951899971545695
6	0.0
7	3.743987496443212E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008823897804117298
16-17	0.0030207171846303184
18-19	0.0
20-21	5.956343744341473E-5
22-23	8.849424991593046E-4
24-25	0.0
26-27	1.0210874990299669E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.829078121362376E-4
36-37	0.0
38-39	0.0
40-41	4.594893745634851E-4
42-43	8.253790617158898E-4
44-45	0.0
46-47	0.0
48-49	5.871253119422309E-4
50-51	0.0
52-53	0.0
54-55	3.829078121362376E-4
56-57	8.50906249191639E-5
58-59	2.1272656229790975E-4
60-61	1.1061781239491308E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015911946859883653
70-71	8.849424991593046E-4
72-73	0.0
74-75	2.637809372494081E-4
76-77	0.0030632624970899007
78-79	5.786162494503146E-4
80-81	0.003480206559193804
82-83	0.002033665935568017
84-85	0.006083979681720219
86-87	0.008160190929747818
88-89	0.008381426554537646
90-91	0.006381796868937292
92-93	0.00945356842851911
94-95	0.001940066248156937
96-97	0.0049607834327872555
98-99	0.0021698109354386795
100	0.08362706617055429
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5876088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.172416551717145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.06472393927109	12.282755736344706
2	16.221954283260104	6.220281296031497
3	6.986886536336499	4.018664972226828
4	3.7240725182831467	2.8559787835730703
5	2.19425804064057	2.103461458855785
6	1.4283692243735082	1.6431173855605214
7	0.9775309899036208	1.3119141931451235
8	0.7106961875978256	1.090061068027426
9	0.5371204577237928	0.9268107438536581
>10	2.439427685985408	8.421922266368156
>50	0.24932138620293037	3.383288195897148
>100	0.3216578830397008	13.712395192430032
>500	0.07154659624651445	9.439330579945583
>1k	0.06710822178744656	23.8040250551615
>5k	0.00434960696770487	5.765721795836539
>10k+	9.764423805051749E-4	3.020271276742488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	29403	0.5003839288996352	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	21026	0.35782309591006806	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	19433	0.33071322281082244	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	16989	0.2891209253503351	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	16934	0.2881849284762243	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	16711	0.2843898866048296	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	14207	0.24177650164531234	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11149	0.18973507544475168	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	10898	0.18546352607380967	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10491	0.1785371492053897	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	10225	0.17401032795969018	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	9801	0.1667946429665451	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9764	0.16616497234214328	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9513	0.16189342297120124	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	9301	0.1582855804746287	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	9224	0.15697518485087358	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	9129	0.15535846297740946	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8886	0.1512230586063381	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8829	0.1502530254822596	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	8210	0.1397188061172671	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8173	0.13908913549286533	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	8113	0.13806804799383537	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	8047	0.1369448517449024	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8013	0.13636623549545207	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7960	0.13546427487130894	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7683	0.13075025425078726	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7582	0.12903142362742015	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7497	0.12758488300379436	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7420	0.12627448738003924	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	7388	0.1257299073805566	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7153	0.12173064800935589	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6923	0.11781647926307434	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6873	0.11696557301388272	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6690	0.1138512561418413	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	6461	0.1099541055205436	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6386	0.10867774614675614	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	6378	0.10854160114688548	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6319	0.10753753177283934	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6319	0.10753753177283934	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	6258	0.10649942614882554	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6133	0.10437216052584646	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	6130	0.10432110615089495	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6129	0.10430408802591111	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	6070	0.10330001865186497	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5974	0.10166627865341703	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5908	0.10054308240448406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5886	0.10016868365483975	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.701812498383278E-5	1.701812498383278E-5
2	0.0	0.0	0.0	1.1912687488682947E-4	1.701812498383278E-5
3	0.0	0.0	0.0	1.1912687488682947E-4	1.701812498383278E-5
4	0.0	0.0	0.0	1.1912687488682947E-4	1.701812498383278E-5
5	0.0	0.0	0.0	1.3614499987066225E-4	3.403624996766556E-5
6	0.0	0.0	0.0	1.3614499987066225E-4	6.807249993533112E-5
7	0.0	0.0	0.0	1.3614499987066225E-4	6.807249993533112E-5
8	0.0	0.0	0.0	1.3614499987066225E-4	6.807249993533112E-5
9	0.0	0.0	0.0	1.3614499987066225E-4	6.807249993533112E-5
10-11	0.0	0.0	0.0	2.2123562478982615E-4	8.50906249191639E-5
12-13	0.0	0.0	0.0	2.552718747574917E-4	1.53163124854495E-4
14-15	0.0	0.0	0.0	2.722899997413245E-4	1.786903123302442E-4
16-17	0.0	0.0	0.0	2.807990622332409E-4	1.871993748221606E-4
18-19	0.0	0.0	0.0	2.893081247251573E-4	1.95708437314077E-4
20-21	0.0	0.0	0.0	2.893081247251573E-4	2.2123562478982615E-4
22-23	0.0	0.0	0.0	3.573806246604884E-4	2.2123562478982615E-4
24-25	0.0	0.0	0.0	5.701071869583982E-4	2.3825374977365894E-4
26-27	0.0	0.0	0.0	8.679243741754718E-4	2.552718747574917E-4
28-29	0.0	0.0	0.0	0.001829448435762024	2.722899997413245E-4
30-31	0.0	0.0	0.0	0.006466887493856457	2.722899997413245E-4
32-33	0.0	0.0	0.0	0.018507210919918148	2.807990622332409E-4
34-35	0.0	0.0	0.0	0.03731223902705337	3.2334437469282287E-4
36-37	0.0	0.0	0.0	0.06974878524623865	3.2334437469282287E-4
38-39	0.0	0.0	0.0	0.12274322644589394	3.48871562168572E-4
40-41	0.0	0.0	0.0	0.17943060076704093	3.573806246604884E-4
42-43	0.0	0.0	0.0	0.24177650164531234	3.573806246604884E-4
44-45	0.0	0.0	0.0	0.3196003871963796	3.829078121362376E-4
46-47	0.0	0.0	0.0	0.4174460967909262	4.0843499961198677E-4
48-49	0.0	0.0	0.0	0.5333395279308275	4.169440621039031E-4
50-51	0.0	0.0	0.0	0.6577760578126128	4.594893745634851E-4
52-53	0.0	0.0	0.0	0.7808256105082156	5.105437495149834E-4
54-55	0.0	0.0	0.0	0.9174130816284576	5.275618744988162E-4
56-57	0.0	0.0	0.0	1.0868880792799565	5.275618744988162E-4
58-59	0.0	0.0	0.0	1.2942454231454668	5.530890619745653E-4
60-61	0.0	0.0	0.0	1.5118902235637042	5.786162494503146E-4
62-63	0.0	0.0	0.0	1.7405457508464814	5.786162494503146E-4
64-65	0.0	0.0	0.0	1.9841772281150316	6.041434369260638E-4
66-67	0.0	0.0	0.0	2.263180878162478	6.381796868937293E-4
68-69	0.0	0.0	0.0	2.5769865938018626	6.637068743694784E-4
70-71	0.0	0.0	0.0	2.925832628782959	6.722159368613948E-4
72-73	0.0	0.0	0.0	3.2954152490568553	7.147612493209768E-4
74-75	0.0	0.0	0.0	3.662964203395184	7.487974992886424E-4
76-77	0.0	0.0	0.0	4.087277113617087	7.743246867643915E-4
78-79	0.0	0.0	0.0	4.53938913100008	7.913428117482244E-4
80-81	0.0	0.0	0.0	5.036446016465376	7.998518742401408E-4
82-83	0.0	0.0	0.0	5.551278333476285	8.423971866997227E-4
84-85	0.0	0.0	0.0	6.056129520184177	8.50906249191639E-4
86-87	0.0	0.0	0.0	6.595365828421903	8.594153116835554E-4
88	0.0	0.0	0.0	7.03173608019485	8.679243741754718E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12150	0.0	41.56234	1
GGGCACC	6795	0.0	41.25712	94
GTGGTAT	2595	0.0	37.86874	1
GTATCAA	20540	0.0	37.3358	1
GTACTAG	7120	0.0	33.745266	1
CAGTACG	985	0.0	33.406723	9
CAACGCA	22295	0.0	32.610886	5
ATCAACG	22550	0.0	32.25062	3
CTGTGCG	9580	0.0	31.011568	9
TAGTACT	10175	0.0	30.16933	4
TAGGCAT	8920	0.0	29.347431	5
TATCAAC	26130	0.0	29.255331	2
TACCCCG	370	0.0	29.214996	5
ACTGTGC	10585	0.0	29.125555	8
AACGCAG	26530	0.0	28.431452	6
GTACTGT	10900	0.0	27.766468	6
TACTGTG	11905	0.0	27.514692	7
CTAGGCA	10010	0.0	26.909615	4
ACGCAGA	27050	0.0	26.877216	7
CGCAGAG	27190	0.0	26.66969	8
>>END_MODULE