FastQCFastQC Report
Mon 16 Jan 2017
ERR1146471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4226322
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130330.3083768818372098No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC102600.24276427588811267No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT84160.19913295768755906No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA65780.15564360689980555No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC59630.1410919470877988No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA55210.13063368101152728No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53250.12599607886005845No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG49540.11721776050192105No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45510.10768228260884996No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT42950.10162500632938049No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC42860.10141205521018037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73800.036.7650031
CAACGCA70250.034.5736355
GTACTAG33700.033.605041
ATCAACG73400.032.9697343
TATCAAC88500.030.1060922
AACGCAG88900.028.8838926
GGGCACC39350.028.33040294
TAGTACT45700.027.5528164
ACGCAGA88150.027.4274887
GCATCGT37900.027.2511358
TAGGCAT44350.026.9054935
GTACATG158600.026.8365381
GGTATCA43450.026.6072141
CGCAGAG91000.026.3105498
TACATGG160550.026.1662712
ACATGGG159150.025.7167323
CTAGGCA48800.025.7062574
CTGTGCG47100.025.5191769
CTAGTAC51700.025.267283
TCAACGC97850.024.7762874
Produced by FastQC (version 0.11.5)