FastQCFastQC Report
Mon 16 Jan 2017
ERR1146471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4226322
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC115490.27326360840466013No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT83190.19683781784729132No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA71190.16844433528727817No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC65220.1543185777136716No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC65180.1542239327718049No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA62070.14686528854166814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.14218036391926597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.12180804018245653No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC48800.11546682907738691No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG47540.11248551340858552No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT43040.1018379574485806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.040.589461
GTATCAA119350.039.4318961
CAACGCA138300.032.6231425
ATCAACG140050.032.32423
TATCAAC154050.030.4255642
AACGCAG158700.029.2877356
GTACTAG30350.028.9680271
TCAACGC165300.027.6990954
GGGCACC39300.026.93257994
ACGCAGA167850.026.6832057
CGCAGAG170400.026.3114768
TAGTACT48150.025.2861634
CTGTGCG47450.025.1620589
TAGGCAT41250.024.3818425
GCAGAGT186900.023.6662249
CTAGTAC52900.023.4602623
GTGGTAT24100.023.4099371
GTACATG152800.023.0459421
ACTGTGC61550.022.4484168
GTACTGT56500.022.2090136
Produced by FastQC (version 0.11.5)