##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4226322
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800429072843954	31.0	31.0	34.0	30.0	34.0
2	31.764879013004688	31.0	31.0	34.0	30.0	34.0
3	31.97238662837332	31.0	31.0	34.0	30.0	34.0
4	35.72369450316374	37.0	35.0	37.0	35.0	37.0
5	35.6086961665486	37.0	35.0	37.0	33.0	37.0
6	35.64896474996463	37.0	35.0	37.0	33.0	37.0
7	35.57416495950853	37.0	35.0	37.0	33.0	37.0
8	35.55691710191509	37.0	35.0	37.0	33.0	37.0
9	37.147320530712044	39.0	37.0	39.0	33.0	39.0
10-11	37.097394495734115	39.0	37.0	39.0	33.0	39.0
12-13	37.037567416775154	39.0	37.0	39.0	33.0	39.0
14-15	38.25090965619752	40.0	38.0	41.0	33.0	41.0
16-17	38.2438739878315	40.0	37.5	41.0	33.0	41.0
18-19	38.287875012836224	40.0	38.0	41.0	33.0	41.0
20-21	38.29872297946063	40.0	38.0	41.0	33.0	41.0
22-23	38.26978860105785	40.0	38.0	41.0	33.0	41.0
24-25	38.20575810361823	40.0	37.0	41.0	33.0	41.0
26-27	38.07262709750937	40.0	37.0	41.0	33.0	41.0
28-29	37.99940539315273	40.0	37.0	41.0	33.0	41.0
30-31	37.88475795265955	40.0	37.0	41.0	33.0	41.0
32-33	37.78064544064556	40.0	37.0	41.0	32.5	41.0
34-35	37.69482460162761	40.0	36.5	41.0	32.0	41.0
36-37	37.6138691041525	40.0	36.0	41.0	32.0	41.0
38-39	37.50453183642893	40.0	36.0	41.0	32.0	41.0
40-41	37.38620460059598	40.0	36.0	41.0	31.0	41.0
42-43	37.26552946036767	39.5	36.0	41.0	31.0	41.0
44-45	37.10271720895852	39.0	35.5	41.0	31.0	41.0
46-47	36.907009806635656	39.0	35.0	41.0	30.5	41.0
48-49	36.6887147974054	39.0	35.0	41.0	30.0	41.0
50-51	36.50524676065856	39.0	35.0	41.0	30.0	41.0
52-53	36.32061388128969	38.5	35.0	41.0	30.0	41.0
54-55	36.11854077848304	38.0	35.0	40.0	29.0	41.0
56-57	35.8796447596752	38.0	34.5	40.0	29.0	41.0
58-59	35.62288237857882	37.0	34.0	40.0	28.5	41.0
60-61	35.36050341171354	37.0	34.0	40.0	28.0	41.0
62-63	35.082902107316954	36.5	34.0	40.0	28.0	41.0
64-65	34.80328131647328	36.0	34.0	39.0	28.0	41.0
66-67	34.56731929086331	35.5	33.0	39.0	28.0	41.0
68-69	34.357799642336765	35.0	33.0	39.0	28.0	41.0
70-71	34.10982279627534	35.0	33.0	38.0	27.5	40.0
72-73	33.88098587850145	35.0	33.0	37.0	27.0	40.0
74-75	33.68015144137148	35.0	33.0	37.0	27.0	39.0
76-77	33.50822500036675	35.0	33.0	36.0	27.0	39.0
78-79	33.384302000652106	35.0	33.0	36.0	27.0	39.0
80-81	33.220900584479836	35.0	33.0	36.0	27.0	37.5
82-83	33.03245079291166	35.0	33.0	35.0	27.0	37.0
84-85	32.888039411100245	35.0	33.0	35.0	27.0	37.0
86-87	32.749016047523114	35.0	33.0	35.0	27.0	36.0
88-89	32.6351674103393	35.0	33.0	35.0	27.0	36.0
90-91	32.54859130468526	35.0	33.0	35.0	27.0	36.0
92-93	32.47955752070003	35.0	33.0	35.0	27.0	35.5
94-95	32.435133669417525	35.0	33.0	35.0	27.0	35.0
96-97	32.38009018243286	35.0	33.0	35.0	27.0	35.0
98-99	32.337607498908035	35.0	33.0	35.0	26.0	35.0
100	31.83113733406967	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47454414560686686
1101	2	0.5612953400675593
1101	3	0.47764743800201614
1101	4	0.3456348265659983
1101	5	0.41725460417953286
1101	6	0.4194901495329759
1101	7	0.409497649506136
1101	8	0.38014753003771773
1101	9	0.5039303829037038
1101	10-11	0.5324055875486664
1101	12-13	0.5323442076578289
1101	14-15	0.6973327715150361
1101	16-17	0.6939843431623629
1101	18-19	0.7297633709359275
1101	20-21	0.6951721939062594
1101	22-23	0.678268241148281
1101	24-25	0.7053329382465918
1101	26-27	0.6588371136209119
1101	28-29	0.7021580705052557
1101	30-31	0.7489772728370099
1101	32-33	0.7416413613543824
1101	34-35	0.7255430668913334
1101	36-37	0.7859285091496915
1101	38-39	0.712572951418835
1101	40-41	0.7698902237000311
1101	42-43	0.7930381144884464
1101	44-45	0.7380809924790768
1101	46-47	0.7879663132679724
1101	48-49	0.7711513678619681
1101	50-51	0.7386077260610335
1101	52-53	0.7557744178002892
1101	54-55	0.7574897034531958
1101	56-57	0.7379127226294813
1101	58-59	0.7122081068015689
1101	60-61	0.7591967824510348
1101	62-63	0.6988334250856312
1101	64-65	0.7295201854120847
1101	66-67	0.7866639738420744
1101	68-69	0.7401436997033457
1101	70-71	0.683683243914615
1101	72-73	0.6588057075454046
1101	74-75	0.5951440905568219
1101	76-77	0.6423459907877742
1101	78-79	0.6353476146983184
1101	80-81	0.5680576375204538
1101	82-83	0.5756535491359571
1101	84-85	0.5546368794971457
1101	86-87	0.571954145911171
1101	88-89	0.5496799856815784
1101	90-91	0.5385435760755399
1101	92-93	0.5254938726728895
1101	94-95	0.46302677576674967
1101	96-97	0.5547921984956687
1101	98-99	0.5809007203874685
1101	100	0.5688852659200307
1102	1	0.4285221190146693
1102	2	0.5326209148691561
1102	3	0.4257556995787155
1102	4	0.3322203318089194
1102	5	0.2932688649798436
1102	6	0.3455635307730276
1102	7	0.3409676923230407
1102	8	0.4045986674207285
1102	9	0.40550463266344394
1102	10-11	0.4437199044577369
1102	12-13	0.4819186262642745
1102	14-15	0.581489729656866
1102	16-17	0.6098123097661912
1102	18-19	0.5742893718827062
1102	20-21	0.5719601185913703
1102	22-23	0.5898713418175419
1102	24-25	0.6200674369241455
1102	26-27	0.6165463389730519
1102	28-29	0.6367138422484047
1102	30-31	0.7071314555129504
1102	32-33	0.6240407886633719
1102	34-35	0.6406882499994211
1102	36-37	0.6228024993943606
1102	38-39	0.6258945756701522
1102	40-41	0.6072243983114234
1102	42-43	0.7000537552636175
1102	44-45	0.6736122466592889
1102	46-47	0.6119103997591111
1102	48-49	0.6384772875849976
1102	50-51	0.6281381558607038
1102	52-53	0.6134627396992016
1102	54-55	0.5702916422219815
1102	56-57	0.6394494107438007
1102	58-59	0.5766057481133515
1102	60-61	0.5546065831348272
1102	62-63	0.5635781200995282
1102	64-65	0.5737382603746255
1102	66-67	0.5008588111806347
1102	68-69	0.5445532850883126
1102	70-71	0.5698261385330738
1102	72-73	0.5121460461215932
1102	74-75	0.474753656165916
1102	76-77	0.5230528310838451
1102	78-79	0.502330311573381
1102	80-81	0.4419744333921045
1102	82-83	0.5086114571561353
1102	84-85	0.47455743914687076
1102	86-87	0.47138017794809883
1102	88-89	0.4151104879157188
1102	90-91	0.4741064890126978
1102	92-93	0.4349453584467895
1102	94-95	0.4819347997052006
1102	96-97	0.43584159907059217
1102	98-99	0.4197203388131854
1102	100	0.40798989748534353
1103	1	0.2758010239620283
1103	2	-0.06342967325739224
1103	3	0.2729627848277332
1103	4	0.2682667297535488
1103	5	0.280780211949029
1103	6	0.305733101302593
1103	7	0.37211040717380683
1103	8	0.3625350131949716
1103	9	0.44667524387499924
1103	10-11	0.42272432049273334
1103	12-13	0.42774793367846087
1103	14-15	0.5581565367204675
1103	16-17	0.5465155036664342
1103	18-19	0.5427449580282229
1103	20-21	0.5588527891010671
1103	22-23	0.5675739178066905
1103	24-25	0.5531181337801456
1103	26-27	0.5509325884215102
1103	28-29	0.6193494118662599
1103	30-31	0.6215662964558959
1103	32-33	0.6533062911514094
1103	34-35	0.6220957126512019
1103	36-37	0.6129426349575624
1103	38-39	0.6495193555942507
1103	40-41	0.6737406970624065
1103	42-43	0.7722942370426935
1103	44-45	0.7567038108740505
1103	46-47	0.7263723588721049
1103	48-49	0.7552932366834213
1103	50-51	0.7897568943384456
1103	52-53	0.6956657789488503
1103	54-55	0.7129237114627855
1103	56-57	0.6982206376740905
1103	58-59	0.6899379233777054
1103	60-61	0.6919007502001335
1103	62-63	0.7105941412497216
1103	64-65	0.6610735071223175
1103	66-67	0.709483480498939
1103	68-69	0.6244281094522464
1103	70-71	0.6416432445483338
1103	72-73	0.5098989339203186
1103	74-75	0.5418210458060173
1103	76-77	0.5647103052899851
1103	78-79	0.5003282726741176
1103	80-81	0.49048283775344714
1103	82-83	0.4956982556438376
1103	84-85	0.5450200315767759
1103	86-87	0.5026016500479855
1103	88-89	0.5107544022467891
1103	90-91	0.500203036808422
1103	92-93	0.5026983064861597
1103	94-95	0.5474137864811013
1103	96-97	0.5409508015514817
1103	98-99	0.5483225447864584
1103	100	0.5224595595016979
1104	1	0.33491367916494497
1104	2	0.41234886788621594
1104	3	0.34392717262494443
1104	4	0.26491371587983537
1104	5	0.23397352772395408
1104	6	0.2357328980736142
1104	7	0.2869048569183761
1104	8	0.2523589395490191
1104	9	0.32795694705490064
1104	10-11	0.3423855517211365
1104	12-13	0.3038998708325664
1104	14-15	0.4115887834706129
1104	16-17	0.4064787514115409
1104	18-19	0.44405093722170363
1104	20-21	0.41113634771828345
1104	22-23	0.43604207002432105
1104	24-25	0.4198859969465971
1104	26-27	0.446115718148711
1104	28-29	0.42789007118621925
1104	30-31	0.4899463227276897
1104	32-33	0.4477411298637506
1104	34-35	0.4592047809347406
1104	36-37	0.45935392111304196
1104	38-39	0.42102018861686474
1104	40-41	0.38835552938576967
1104	42-43	0.3552843992708219
1104	44-45	0.3935523304806665
1104	46-47	0.4566397861051996
1104	48-49	0.4405624173069427
1104	50-51	0.4574619025298219
1104	52-53	0.47833370794132435
1104	54-55	0.4236709233942406
1104	56-57	0.3363715163079135
1104	58-59	0.3782841612706278
1104	60-61	0.3453123473487949
1104	62-63	0.3288980767668548
1104	64-65	0.30614674666571773
1104	66-67	0.2832095483050665
1104	68-69	0.2795720894952538
1104	70-71	0.2333435941358033
1104	72-73	0.2782368898720975
1104	74-75	0.2750721795042139
1104	76-77	0.33060207860502544
1104	78-79	0.27638456969513925
1104	80-81	0.2880796406877906
1104	82-83	0.28056469697136777
1104	84-85	0.24239536537316297
1104	86-87	0.2775035656420428
1104	88-89	0.2748625049764968
1104	90-91	0.2364837956702317
1104	92-93	0.2848546077303169
1104	94-95	0.2672778238932949
1104	96-97	0.3008517401570643
1104	98-99	0.2871864976880758
1104	100	0.33688500692133516
1105	1	0.12004338649404644
1105	2	-0.3769598572021735
1105	3	0.0483534065170943
1105	4	0.10022153265007461
1105	5	0.1860983727853096
1105	6	0.21398187827278292
1105	7	0.2717667543890485
1105	8	0.21854644210609564
1105	9	0.30407238528641045
1105	10-11	0.36301575629386207
1105	12-13	0.305300849868793
1105	14-15	0.3937975645346228
1105	16-17	0.39497090442498006
1105	18-19	0.3662921151188101
1105	20-21	0.39782325840574373
1105	22-23	0.4320458015799673
1105	24-25	0.445542899078859
1105	26-27	0.41066928800589864
1105	28-29	0.39496428105343284
1105	30-31	0.40263537187126275
1105	32-33	0.40432124802739366
1105	34-35	0.3949936131927956
1105	36-37	0.3791500912286452
1105	38-39	0.43552961110342636
1105	40-41	0.4672262869583079
1105	42-43	0.4637709341513627
1105	44-45	0.46437615264414234
1105	46-47	0.42734555433687405
1105	48-49	0.4305997625768683
1105	50-51	0.41939224088176275
1105	52-53	0.4438875020209849
1105	54-55	0.4485204532013185
1105	56-57	0.47271228783892383
1105	58-59	0.46935959259032245
1105	60-61	0.4270261018775301
1105	62-63	0.3695020466245822
1105	64-65	0.45457307518387324
1105	66-67	0.41872097096580063
1105	68-69	0.43027961310775
1105	70-71	0.4472062799278902
1105	72-73	0.4170680736638914
1105	74-75	0.3354280463960606
1105	76-77	0.3089749182923569
1105	78-79	0.26634639701915575
1105	80-81	0.37360333730038064
1105	82-83	0.28517163451981276
1105	84-85	0.3159386907089967
1105	86-87	0.28688613329335055
1105	88-89	0.34740801745284244
1105	90-91	0.3245543144378331
1105	92-93	0.38062671130052195
1105	94-95	0.27881325870089313
1105	96-97	0.29676387420416717
1105	98-99	0.25323619827907606
1105	100	0.24668190615996366
1106	1	0.2493266555140714
1106	2	0.32108369778528356
1106	3	0.2559517099251494
1106	4	0.20159116199332772
1106	5	0.19685295668654135
1106	6	0.23242948205268732
1106	7	0.25837245387411656
1106	8	0.2348740354043457
1106	9	0.27889183201428835
1106	10-11	0.2394857834126256
1106	12-13	0.2988484025309788
1106	14-15	0.3732729879110792
1106	16-17	0.3364484140326951
1106	18-19	0.3603867563998122
1106	20-21	0.3706201605935959
1106	22-23	0.3026645055017809
1106	24-25	0.3368565907150298
1106	26-27	0.35221732996169663
1106	28-29	0.34761665084493387
1106	30-31	0.36169945194035336
1106	32-33	0.3401752477502029
1106	34-35	0.28038062368175076
1106	36-37	0.33071602719066107
1106	38-39	0.28820930727004423
1106	40-41	0.27956815730559725
1106	42-43	0.2119750526344859
1106	44-45	0.2608274272483939
1106	46-47	0.20967745364339407
1106	48-49	0.24122759025349438
1106	50-51	0.2813902870739966
1106	52-53	0.3316413020424136
1106	54-55	0.3266096381851682
1106	56-57	0.26389142373069063
1106	58-59	0.3256210078047701
1106	60-61	0.28603356528852686
1106	62-63	0.29629283600893075
1106	64-65	0.2580685506207132
1106	66-67	0.22513902186019408
1106	68-69	0.216519857395447
1106	70-71	0.24855144459034761
1106	72-73	0.27187339147749157
1106	74-75	0.22850661786070248
1106	76-77	0.18930559548706327
1106	78-79	0.2346887072818049
1106	80-81	0.22499341934123152
1106	82-83	0.25127682928055606
1106	84-85	0.21322933953365464
1106	86-87	0.2348112085250449
1106	88-89	0.2411813634075486
1106	90-91	0.17730465517584548
1106	92-93	0.17381705717836127
1106	94-95	0.1497835021300773
1106	96-97	0.18461985354609567
1106	98-99	0.2346140311460232
1106	100	0.2708871266759587
1107	1	0.07421249138637265
1107	2	-0.4814999758178118
1107	3	-0.0023953605848525683
1107	4	0.03547802901817221
1107	5	0.07902285893391792
1107	6	0.11808106856198464
1107	7	0.13963899530322266
1107	8	0.11875152264755684
1107	9	0.16657384936898012
1107	10-11	0.22837244810622792
1107	12-13	0.18762057154569334
1107	14-15	0.23717800127117528
1107	16-17	0.36566199613786665
1107	18-19	0.3704559831555585
1107	20-21	0.28347392996794696
1107	22-23	0.24593584688536652
1107	24-25	0.23473015171200018
1107	26-27	0.30216975094851506
1107	28-29	0.30140931879148525
1107	30-31	0.3133340900060304
1107	32-33	0.32738005808761983
1107	34-35	0.36169960120733435
1107	36-37	0.35095419543208806
1107	38-39	0.41018306949875694
1107	40-41	0.35777699193772605
1107	42-43	0.3346824441404479
1107	44-45	0.3212507903518187
1107	46-47	0.3630745951846919
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2216	82-83	-0.5264186504808137
2216	84-85	-0.5908046588979374
2216	86-87	-0.49666394449501183
2216	88-89	-0.5596930383497636
2216	90-91	-0.6177398701337538
2216	92-93	-0.6388747300371733
2216	94-95	-0.5792419389166703
2216	96-97	-0.5204901059298237
2216	98-99	-0.5609401294590555
2216	100	-0.5457162205891706
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	45.0
14	170.0
15	624.0
16	1409.0
17	3040.0
18	5421.0
19	8363.0
20	12337.0
21	17282.0
22	24044.0
23	28957.0
24	32075.0
25	35790.0
26	42025.0
27	52262.0
28	66487.0
29	82377.0
30	102129.0
31	126312.0
32	156590.0
33	197875.0
34	265690.0
35	365385.0
36	552306.0
37	928438.0
38	851529.0
39	266863.0
40	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53110090533834	20.46627397553975	13.7149636049585	25.287661514163407
2	15.045919014437775	20.168399691822504	39.168237841848914	25.617443451890804
3	18.30313791334536	25.458018554519278	29.357837022342277	26.88100650979308
4	12.828922766401998	16.830597497465185	37.41369549432021	32.92678424181261
5	13.46721414075113	37.95476169236787	34.44960090596818	14.128423260912825
6	31.118097485236575	37.54938691372782	17.377426518850196	13.95508908218541
7	27.143504111393785	31.708533035011204	22.887657868435397	18.26030498515961
8	26.327359817827418	34.78222908713534	19.564126916974146	19.326284178063098
9	26.009281829448867	15.141463428484625	20.711602192166144	38.137652549900366
10-11	25.698456009740855	25.683715060045113	27.17582806042701	21.442000869787016
12-13	26.165244863027475	23.199486456545433	27.61509889686588	23.020169783561215
14-15	23.386903842135638	24.478703877015313	25.148457497319544	26.98593478352951
16-17	22.6360319288856	27.14484119261148	26.225434666022434	23.99369221248049
18-19	22.222514044126314	26.858246957993266	28.44027265314853	22.478966344731894
20-21	23.439448910571006	26.087615950474163	27.900481731208444	22.572453407746384
22-23	23.574141607734923	25.703965951204218	27.732069813045136	22.989822628015723
24-25	23.211541856015703	25.811982617509976	28.213467880582694	22.763007645891626
26-27	23.134916888550375	26.061569505145503	27.500471451120635	23.30304215518349
28-29	22.61575194696476	26.44706200805334	27.884387417712137	23.052798627269762
30-31	23.220568617346242	26.092829651881704	27.775238138504356	22.911363592267698
32-33	23.373526674020578	26.25028334329471	27.240423233251036	23.135766749433667
34-35	23.426420744498166	26.141658541940917	27.762888855526345	22.669031858034568
36-37	23.292439620076273	25.816430929777713	27.45448643051807	23.436643019627944
38-39	23.369977488700577	25.84004484277346	27.880944707951738	22.90903296057423
40-41	23.09244944171156	25.336558368884443	28.284780340296855	23.28621184910714
42-43	23.218591028812106	25.462222066057127	27.66106211431214	23.658124790818626
44-45	23.268766553991863	26.024590648795808	27.5033232205213	23.203319576691033
46-47	23.37222530606991	26.00295244896153	26.93229479438623	23.692527450582325
48-49	23.964582761271167	25.918252203136856	26.813999515179166	23.303165520412815
50-51	23.5585930272232	25.978072659868324	26.753451346111344	23.709882966797135
52-53	23.24228963150465	26.207835086867494	26.638161976299962	23.911713305327893
54-55	23.165388147744505	26.183304843149795	26.60099786823469	24.05030914087101
56-57	23.836907758996006	25.90080732173617	26.38380434988359	23.878480569384237
58-59	23.01760517140819	26.127359176966223	27.031077901197616	23.823957750427972
60-61	23.13008716803271	26.514556398947548	26.614418690491963	23.74093774252778
62-63	23.474359028961825	26.022023404747674	26.259854313040986	24.243763253249515
64-65	22.847442764654467	26.05462858722076	26.66984437058984	24.428084277534936
66-67	23.271842514602533	25.850313818965994	26.637854380238895	24.239989286192582
68-69	22.983067811569065	25.8233146486145	26.85953571352517	24.334081826291268
70-71	22.8901001148405	25.595246334468015	26.87311455786408	24.641538992827407
72-73	23.312847435666285	25.624218883464156	27.000592950560794	24.062340730308765
74-75	23.366023742663824	25.966965082592026	26.700239641333017	23.966771533411134
76-77	23.22253333565219	25.88975226907932	26.345230375570978	24.542484019697515
78-79	23.01806732312678	25.971073069992123	26.47502403991762	24.535835566963474
80-81	22.80260522239405	26.273651990492176	26.824891418503626	24.09885136861015
82-83	23.40667610529094	25.561836535284282	27.409620558549342	23.62186680087543
84-85	23.492031228389454	25.91182086611728	26.4860395204504	24.110108385042867
86-87	22.637500559039147	26.32177559825471	26.770166405545098	24.27055743716104
88-89	23.160989223435184	25.456291992800793	26.69504560080378	24.68767318296024
90-91	22.839289850994973	25.536623727934437	26.7743727726208	24.849713648449786
92-93	23.133520808674348	25.682587557247103	26.641407030514593	24.542484603563956
94-95	22.346823968681566	25.54562953340597	27.15784504757747	24.949701450334988
96-97	22.645535597453083	25.41690023333399	26.837123600759185	25.100440568453745
98-99	22.215328788399344	25.866312971991206	26.70555699103033	25.212801248579115
100	22.68857156389126	25.29073126835275	26.328099838969404	25.692597328786587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	107.0
1	85.0
2	95.0
3	125.5
4	84.5
5	73.0
6	176.5
7	496.0
8	832.5
9	922.0
10	726.0
11	528.5
12	516.5
13	551.5
14	706.0
15	918.0
16	1108.0
17	1380.5
18	1826.5
19	2326.0
20	2818.5
21	3434.0
22	4285.5
23	5274.0
24	6245.0
25	7344.5
26	9085.0
27	11358.0
28	14707.5
29	21095.5
30	29852.5
31	38608.0
32	50207.0
33	60871.0
34	69133.5
35	78207.5
36	87182.5
37	103142.0
38	112769.5
39	111885.0
40	110382.5
41	108311.5
42	110389.0
43	118052.5
44	129930.0
45	151476.0
46	172297.0
47	186544.5
48	195526.0
49	192757.0
50	186970.5
51	190632.5
52	193345.5
53	189493.5
54	184255.5
55	166002.5
56	147751.0
57	126478.0
58	105135.0
59	97569.0
60	82164.5
61	62108.5
62	48236.5
63	38017.0
64	27633.0
65	17097.5
66	11604.5
67	8669.0
68	6667.5
69	4982.0
70	3833.5
71	2919.5
72	2138.5
73	1748.5
74	1386.5
75	1025.0
76	729.0
77	474.5
78	280.5
79	129.0
80	72.0
81	33.5
82	14.0
83	8.5
84	2.5
85	3.5
86	3.0
87	1.0
88	1.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014409692399206685
2	0.004400989796802042
3	0.0017509314245341457
4	0.02758900055414613
5	0.0029576544333347055
6	0.0
7	4.259022384001976E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00863635094533734
16-17	0.0027801951673346237
18-19	0.0
20-21	5.915308866669411E-5
22-23	8.163126236003788E-4
24-25	0.0
26-27	2.129511192000988E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.0336150440028E-4
36-37	0.0
38-39	0.0
40-41	2.839348256001317E-4
42-43	6.0336150440028E-4
44-45	0.0
46-47	0.0
48-49	4.3773285613353644E-4
50-51	0.0
52-53	0.0
54-55	2.957654433334706E-4
56-57	4.732247093335529E-5
58-59	1.656286482667435E-4
60-61	4.732247093335529E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0012303842442672375
70-71	6.0336150440028E-4
72-73	0.0
74-75	1.4196741280006586E-4
76-77	0.0024489378708011365
78-79	4.259022384001976E-4
80-81	0.002803856402801301
82-83	0.0016681171004007738
84-85	0.005477576010535875
86-87	0.007595256584803524
88-89	0.007098370640003293
90-91	0.005276455509069115
92-93	0.00813946500053711
94-95	0.0013486904216006258
96-97	0.004519295974135431
98-99	0.0018455763664008565
100	0.08333487131363866
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4226322.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.150213772267875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29360691056286	23.663914890472203
2	15.551064109006276	10.621443274176135
3	5.904463942150138	6.049161175052241
4	2.886834615049691	3.943440769165183
5	1.633305086643713	2.7888858932107654
6	1.0007736133800684	2.050597970074458
7	0.6801485981080494	1.6259054015608738
8	0.49005119465679264	1.338828244550778
9	0.3473349547227195	1.067540665892567
>10	1.8170983807672039	11.31038785784921
>50	0.16739909778239048	4.008091049208558
>100	0.17851570261325775	13.243000718777681
>500	0.03328182799081761	7.901581032221011
>1k	0.015568422649517221	9.031097332902899
>5k	4.843509268738691E-4	1.0832260074960025
>10k+	6.919298955340986E-5	0.2728977173895409
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11549	0.27326360840466013	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	8319	0.19683781784729132	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7119	0.16844433528727817	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6522	0.1543185777136716	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6518	0.1542239327718049	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6207	0.14686528854166814	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6009	0.14218036391926597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5148	0.12180804018245653	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4880	0.11546682907738691	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4754	0.11248551340858552	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4304	0.1018379574485806	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.732247093335529E-5
2	0.0	0.0	0.0	0.0	4.732247093335529E-5
3	0.0	0.0	0.0	0.0	4.732247093335529E-5
4	0.0	0.0	0.0	0.0	4.732247093335529E-5
5	0.0	0.0	0.0	0.0	4.732247093335529E-5
6	0.0	0.0	0.0	0.0	1.1830617733338822E-4
7	0.0	0.0	0.0	0.0	1.1830617733338822E-4
8	0.0	0.0	0.0	0.0	1.1830617733338822E-4
9	0.0	0.0	0.0	0.0	1.1830617733338822E-4
10-11	0.0	0.0	0.0	2.3661235466677646E-5	1.4196741280006586E-4
12-13	0.0	0.0	0.0	2.3661235466677646E-5	3.194266788001482E-4
14-15	0.0	0.0	0.0	2.3661235466677646E-5	3.430879142668259E-4
16-17	0.0	0.0	0.0	3.549185320001647E-5	4.0224100293352E-4
18-19	0.0	0.0	0.0	4.732247093335529E-5	4.4956347386687526E-4
20-21	0.0	0.0	0.0	5.915308866669411E-5	5.323777980002469E-4
22-23	0.0	0.0	0.0	9.464494186671058E-5	5.678696512002635E-4
24-25	0.0	0.0	0.0	2.3661235466677645E-4	5.678696512002635E-4
26-27	0.0	0.0	0.0	5.560390334669247E-4	6.151921221336187E-4
28-29	0.0	0.0	0.0	0.001750931424534146	6.151921221336187E-4
30-31	0.0	0.0	0.0	0.0063057192518695925	6.388533576002964E-4
32-33	0.0	0.0	0.0	0.018207320691608447	7.453289172003458E-4
34-35	0.0	0.0	0.0	0.03751488883241741	8.0448200586704E-4
36-37	0.0	0.0	0.0	0.07072343280989948	8.0448200586704E-4
38-39	0.0	0.0	0.0	0.12219845056765671	8.518044768003952E-4
40-41	0.0	0.0	0.0	0.18098479008461732	8.754657122670729E-4
42-43	0.0	0.0	0.0	0.24378170901317978	9.109575654670894E-4
44-45	0.0	0.0	0.0	0.3237566848905502	9.701106541337834E-4
46-47	0.0	0.0	0.0	0.4216668772516623	0.0010056025073338
48-49	0.0	0.0	0.0	0.5329456676514472	0.0010884168314671716
50-51	0.0	0.0	0.0	0.6558302940476377	0.0011120780669338494
52-53	0.0	0.0	0.0	0.7819801709382295	0.00120672300880056
54-55	0.0	0.0	0.0	0.9196885613542934	0.0012422148620005763
56-57	0.0	0.0	0.0	1.0898128443597057	0.0012540454797339152
58-59	0.0	0.0	0.0	1.2904956129703322	0.001277706715200593
60-61	0.0	0.0	0.0	1.5045588102373648	0.001277706715200593
62-63	0.0	0.0	0.0	1.7290921041984022	0.0013486904216006258
64-65	0.0	0.0	0.0	1.966899351256246	0.001396012892533981
66-67	0.0	0.0	0.0	2.2477227243925095	0.0014315047457339977
68-69	0.0	0.0	0.0	2.558288270510387	0.001466996598934014
70-71	0.0	0.0	0.0	2.903612171528814	0.0014788272166673527
72-73	0.0	0.0	0.0	3.26799519771565	0.0016326252472007577
74-75	0.0	0.0	0.0	3.6389205555090216	0.0017154395713341293
76-77	0.0	0.0	0.0	4.05160799390108	0.0017745926600008235
78-79	0.0	0.0	0.0	4.504862620500757	0.0018219151309341788
80-81	0.0	0.0	0.0	5.000482689203521	0.0018574069841341952
82-83	0.0	0.0	0.0	5.515019915661892	0.0019402213082675669
84-85	0.0	0.0	0.0	6.032325506669865	0.0019402213082675669
86-87	0.0	0.0	0.0	6.583868905398122	0.0020112050146676
88	0.0	0.0	0.0	7.024594907818193	0.0020821887210676327
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6695	0.0	40.58946	1
GTATCAA	11935	0.0	39.431896	1
CAACGCA	13830	0.0	32.623142	5
ATCAACG	14005	0.0	32.3242	3
TATCAAC	15405	0.0	30.425564	2
AACGCAG	15870	0.0	29.287735	6
GTACTAG	3035	0.0	28.968027	1
TCAACGC	16530	0.0	27.699095	4
GGGCACC	3930	0.0	26.932579	94
ACGCAGA	16785	0.0	26.683205	7
CGCAGAG	17040	0.0	26.311476	8
TAGTACT	4815	0.0	25.286163	4
CTGTGCG	4745	0.0	25.162058	9
TAGGCAT	4125	0.0	24.381842	5
GCAGAGT	18690	0.0	23.666224	9
CTAGTAC	5290	0.0	23.460262	3
GTGGTAT	2410	0.0	23.409937	1
GTACATG	15280	0.0	23.045942	1
ACTGTGC	6155	0.0	22.448416	8
GTACTGT	5650	0.0	22.209013	6
>>END_MODULE