FastQCFastQC Report
Mon 16 Jan 2017
ERR1146470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659006
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC148970.3197463149865014No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142720.3063314363621768No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT123820.2657648434022193No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA112840.24219758463500585No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC97670.2096369912380452No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA89930.19302400554968163No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC88120.18913905670007722No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC71870.1542603722768333No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT65830.14129623357428603No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG64330.1380766627044481No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC57410.12322370909159594No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC56590.12146367701608456No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT54860.11775043861287149No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT53310.11442354871403901No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG52800.11332889461829411No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT51830.11124690545579895No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC49410.10605266445246048No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA49180.10555899691908532No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT47850.10270431074782904No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG46880.10062232158533388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG41900.036.0245971
GGGCACC45050.033.11630294
CAACGCA86850.032.8859675
GTATCAA95350.032.6502381
TAGGCAT51300.031.9583935
GGTATCA60550.031.4724371
ATCAACG94500.030.1786233
GCATCGT47750.029.0043338
AACGCAG110350.028.0762426
TAGTACT58800.027.8033774
CTGTGCG58000.027.767199
CTAGGCA60500.027.7209724
TATCAAC112950.027.4135462
TATTCTC28450.026.5840115
TACCCCG4100.026.3524425
ACGCAGA108500.026.2214747
GTACATG188100.025.6536981
CTAACGC22600.025.5705013
CTAGTAC67700.025.1224773
GTACTGT66150.025.1212236
Produced by FastQC (version 0.11.5)