##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146470_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4659006
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54817615602985	31.0	31.0	34.0	27.0	34.0
2	30.660618595468648	31.0	31.0	34.0	27.0	34.0
3	30.902815106913362	31.0	31.0	34.0	27.0	34.0
4	34.38390935748956	37.0	35.0	37.0	30.0	37.0
5	34.635975141478674	37.0	35.0	37.0	31.0	37.0
6	34.770553418475956	37.0	35.0	37.0	32.0	37.0
7	34.64989570736763	37.0	35.0	37.0	31.0	37.0
8	34.60369379219516	37.0	35.0	37.0	30.0	37.0
9	36.02002852110515	39.0	35.0	39.0	30.0	39.0
10-11	35.94587075011279	39.0	35.0	39.0	30.0	39.0
12-13	35.94582653467285	39.0	35.0	39.0	30.0	39.0
14-15	37.00188495142526	40.0	36.0	41.0	30.0	41.0
16-17	36.83407222484796	40.0	36.0	41.0	29.5	41.0
18-19	36.77731795151155	40.0	36.0	41.0	29.5	41.0
20-21	37.101108798743766	40.0	36.0	41.0	30.0	41.0
22-23	36.82117237024379	40.0	36.0	41.0	29.5	41.0
24-25	36.938739185998045	40.0	36.0	41.0	30.0	41.0
26-27	37.00887120557475	40.0	36.0	41.0	30.0	41.0
28-29	36.8346409083826	40.0	36.0	41.0	30.0	41.0
30-31	36.77607176294686	40.0	36.0	41.0	30.0	41.0
32-33	36.87700058767901	40.0	36.0	41.0	30.0	41.0
34-35	36.930849734900534	40.0	36.0	41.0	30.0	41.0
36-37	36.870203215020545	40.0	36.0	41.0	30.0	41.0
38-39	36.70314258019843	40.0	35.0	41.0	30.0	41.0
40-41	36.66948228871137	39.0	35.0	41.0	30.0	41.0
42-43	36.5139144487043	39.0	35.0	41.0	29.0	41.0
44-45	36.338098405539725	39.0	35.0	41.0	29.0	41.0
46-47	36.21340388915576	39.0	35.0	41.0	28.5	41.0
48-49	36.0885861705265	39.0	35.0	41.0	28.0	41.0
50-51	35.87610973241932	38.5	34.5	40.5	28.0	41.0
52-53	35.69863797127542	38.0	34.0	40.0	28.0	41.0
54-55	35.50317256513514	38.0	34.0	40.0	28.0	41.0
56-57	35.36720858483548	37.5	34.0	40.0	27.5	41.0
58-59	35.11468959258691	37.0	33.5	40.0	27.0	41.0
60-61	34.85804826179661	36.5	33.0	40.0	26.5	41.0
62-63	34.592782773836305	36.0	33.0	39.5	26.0	41.0
64-65	34.3066457737981	35.5	33.0	39.0	26.0	41.0
66-67	34.01225121839293	35.0	33.0	39.0	26.0	41.0
68-69	33.725284642260604	35.0	32.0	38.5	26.0	40.5
70-71	33.643795479121515	35.0	32.0	38.0	26.0	40.0
72-73	33.424856933002445	35.0	32.0	37.0	26.0	40.0
74-75	33.155925319692656	35.0	32.0	37.0	26.0	39.5
76-77	32.91328804899586	35.0	32.0	36.0	25.0	39.0
78-79	32.68779885666599	35.0	32.0	36.0	25.0	39.0
80-81	32.45586719570655	35.0	32.0	36.0	25.0	37.5
82-83	32.225519992891186	35.0	32.0	35.0	25.0	37.0
84-85	32.04252602379134	35.0	31.0	35.0	25.0	37.0
86-87	31.871934270958228	35.0	31.5	35.0	24.0	36.0
88-89	31.86177716877806	35.0	32.0	35.0	24.5	36.0
90-91	31.788450583665274	35.0	32.0	35.0	24.5	36.0
92-93	31.64998274310014	35.0	32.0	35.0	24.0	36.0
94-95	31.63166123847018	35.0	32.0	35.0	24.0	35.0
96-97	31.545518400276798	35.0	32.0	35.0	24.0	35.0
98-99	31.467036852925283	35.0	32.0	35.0	24.0	35.0
100	30.992918875828877	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8603648832536521
1101	2	1.0207271617611724
1101	3	1.0000130124885978
1101	4	1.0364984060771647
1101	5	0.9112128512573889
1101	6	0.728826986846471
1101	7	0.7469612630188038
1101	8	0.8052720530699986
1101	9	0.9891902582787182
1101	10-11	1.0100690872033482
1101	12-13	0.9360893063244831
1101	14-15	1.163569895781258
1101	16-17	1.320172274993908
1101	18-19	1.4253215268367327
1101	20-21	1.0917411048298575
1101	22-23	1.3687000781159568
1101	24-25	1.2161338635062506
1101	26-27	1.1142886266262408
1101	28-29	1.2732896477657007
1101	30-31	1.3006877347490544
1101	32-33	1.131024781049213
1101	34-35	1.0776854939818747
1101	36-37	1.0037679566182192
1101	38-39	1.0949724489336674
1101	40-41	0.9814143055229678
1101	42-43	1.0048807688237957
1101	44-45	1.1038923240491059
1101	46-47	1.0988513909043363
1101	48-49	1.1327838737497444
1101	50-51	1.038863729652654
1101	52-53	1.1179225363111058
1101	54-55	1.0921180423603758
1101	56-57	1.0257278918679091
1101	58-59	0.9575202808962544
1101	60-61	1.0008190960458876
1101	62-63	0.9378796435081398
1101	64-65	0.8747275374497008
1101	66-67	0.9204674710859706
1101	68-69	0.9269414508427403
1101	70-71	0.9227764716122877
1101	72-73	0.7860423993535051
1101	74-75	0.788028290127329
1101	76-77	0.7714807374869608
1101	78-79	0.7862981034536389
1101	80-81	0.8378878097916029
1101	82-83	0.8097843802546123
1101	84-85	0.8159415973135964
1101	86-87	0.8224136764547474
1101	88-89	0.7924845565504377
1101	90-91	0.8385445511199947
1101	92-93	0.8472255678651912
1101	94-95	0.8275246276696926
1101	96-97	0.7677468632416691
1101	98-99	0.8194241078189961
1101	100	0.92937102614761
1102	1	0.783694035507235
1102	2	0.9099789222591212
1102	3	0.8912853962752685
1102	4	0.9827179161241233
1102	5	0.8231066909985927
1102	6	0.612247378208977
1102	7	0.6820679864455244
1102	8	0.6414374120419524
1102	9	0.7683168378802279
1102	10-11	0.8042021624290783
1102	12-13	0.7616516011375438
1102	14-15	0.9816085889099284
1102	16-17	1.198681396104739
1102	18-19	1.2194742228287865
1102	20-21	1.0031665407758936
1102	22-23	1.230270572311845
1102	24-25	1.0973717780764005
1102	26-27	0.9626917945807136
1102	28-29	1.1638569669000631
1102	30-31	1.184953682374541
1102	32-33	0.9973261199756038
1102	34-35	0.8793244032546212
1102	36-37	0.8512137085593992
1102	38-39	0.9802725850571861
1102	40-41	0.9370691844907313
1102	42-43	0.9343829583160002
1102	44-45	0.9427301189600641
1102	46-47	0.9186516663688948
1102	48-49	0.8383268166020059
1102	50-51	0.9862562805019479
1102	52-53	0.9397505828474522
1102	54-55	0.8772638494983198
1102	56-57	0.8276487007444331
1102	58-59	0.7925224959962591
1102	60-61	0.8089155996701294
1102	62-63	0.7261648852605163
1102	64-65	0.7065292766824882
1102	66-67	0.8042028943686219
1102	68-69	0.8292911979771844
1102	70-71	0.6973834530006613
1102	72-73	0.6856698389799405
1102	74-75	0.7472345990742681
1102	76-77	0.7343496946956733
1102	78-79	0.7415415165220907
1102	80-81	0.7975641312939956
1102	82-83	0.7642815016427988
1102	84-85	0.6702766310115322
1102	86-87	0.7063584931258227
1102	88-89	0.6739623800443901
1102	90-91	0.7159447985636191
1102	92-93	0.7522016772657842
1102	94-95	0.7782561197734772
1102	96-97	0.7790534647487917
1102	98-99	0.7793670355203872
1102	100	0.8159073010957414
1103	1	0.6734974920070229
1103	2	0.8123518807406533
1103	3	0.760996878108358
1103	4	0.7381549014973743
1103	5	0.7059741945246572
1103	6	0.48645953807335474
1103	7	0.5418704462846264
1103	8	0.6273511737111832
1103	9	0.7547383931339837
1103	10-11	0.8063973327523541
1103	12-13	0.6908692124338387
1103	14-15	0.8800862148843152
1103	16-17	1.0726013028414627
1103	18-19	1.1522929000486428
1103	20-21	0.8784094894015624
1103	22-23	1.0728638230062373
1103	24-25	0.9560666861470324
1103	26-27	0.8720544149727942
1103	28-29	1.0090362500493484
1103	30-31	1.0703497341888166
1103	32-33	0.8938741001269577
1103	34-35	0.8050043724890159
1103	36-37	0.8676827713057378
1103	38-39	0.8987590299685237
1103	40-41	0.8089925986676789
1103	42-43	0.839572691301548
1103	44-45	0.9594790520801055
1103	46-47	0.8717468011546643
1103	48-49	0.7839862083168327
1103	50-51	0.8827336386866946
1103	52-53	0.8542523481449322
1103	54-55	0.8810845331270158
1103	56-57	0.7744084461227985
1103	58-59	0.7272182678394898
1103	60-61	0.7726374024782956
1103	62-63	0.6777036186155385
1103	64-65	0.7310796971424196
1103	66-67	0.7595174112851808
1103	68-69	0.7649596703782393
1103	70-71	0.7268283331345913
1103	72-73	0.6686020751140234
1103	74-75	0.6822273556665692
1103	76-77	0.6277678582829296
1103	78-79	0.6447128606146606
1103	80-81	0.6702768201939193
1103	82-83	0.6402361062654904
1103	84-85	0.6498927555719689
1103	86-87	0.7026864528808723
1103	88-89	0.7035118088772769
1103	90-91	0.716636219328592
1103	92-93	0.801913400713584
1103	94-95	0.7330924409076154
1103	96-97	0.7169122248425026
1103	98-99	0.7548007087586441
1103	100	0.7210541978064597
1104	1	0.5697031631383034
1104	2	0.6920633002199779
1104	3	0.6878781849966238
1104	4	0.7978376790600521
1104	5	0.6946185228272128
1104	6	0.45496971938275976
1104	7	0.43819171410959257
1104	8	0.46894792579968225
1104	9	0.5930148839767355
1104	10-11	0.6175155137343822
1104	12-13	0.5650879216166729
1104	14-15	0.7521948523352222
1104	16-17	0.9038466183346188
1104	18-19	0.8688774498224703
1104	20-21	0.7156578470802515
1104	22-23	0.9337741623646778
1104	24-25	0.7941135033046081
1104	26-27	0.7264588297771937
1104	28-29	0.9081064207585854
1104	30-31	0.9291951955733779
1104	32-33	0.7816868334831852
1104	34-35	0.6463650343719607
1104	36-37	0.6873822636060112
1104	38-39	0.7703693441575226
1104	40-41	0.6840745582928704
1104	42-43	0.6648318601217866
1104	44-45	0.7605570473684296
1104	46-47	0.6306427504232346
1104	48-49	0.6158379445815569
1104	50-51	0.7242827337090105
1104	52-53	0.6870307054402289
1104	54-55	0.6713562836908409
1104	56-57	0.5545577881513424
1104	58-59	0.5322581565456446
1104	60-61	0.5735886267921018
1104	62-63	0.5230278922723812
1104	64-65	0.5277873169689542
1104	66-67	0.6124905844690502
1104	68-69	0.5502484366402953
1104	70-71	0.4622959971496954
1104	72-73	0.4872525755926844
1104	74-75	0.46629559248027874
1104	76-77	0.4858909148271735
1104	78-79	0.5216005840233038
1104	80-81	0.5129608149089293
1104	82-83	0.4793733445116928
1104	84-85	0.498955148981743
1104	86-87	0.48317905888407253
1104	88-89	0.42703839800403287
1104	90-91	0.4917999746895134
1104	92-93	0.5139126712565307
1104	94-95	0.44352622946955833
1104	96-97	0.45939059292470574
1104	98-99	0.5090939171201398
1104	100	0.6051870567278357
1105	1	0.5243806003124263
1105	2	0.6343857884983919
1105	3	0.6489933613705894
1105	4	0.7683397692472411
1105	5	0.6635378696670031
1105	6	0.3875885351856283
1105	7	0.49051707190143645
1105	8	0.5058930182297914
1105	9	0.5227936825617405
1105	10-11	0.5911427058799035
1105	12-13	0.5979035046106915
1105	14-15	0.7234632511603394
1105	16-17	0.8946075842099646
1105	18-19	0.8929433889779759
1105	20-21	0.7226994712800803
1105	22-23	0.9103560997672915
1105	24-25	0.7496937541923998
1105	26-27	0.5629977396444161
1105	28-29	0.8524580621891076
1105	30-31	0.8464900746526851
1105	32-33	0.7482221392508634
1105	34-35	0.6226683909793707
1105	36-37	0.5539606023533281
1105	38-39	0.6913878631549366
1105	40-41	0.5967413407407847
1105	42-43	0.5949791378308404
1105	44-45	0.6238935339648037
1105	46-47	0.5430607496727831
1105	48-49	0.6527025591954754
1105	50-51	0.6209168341138209
1105	52-53	0.6302064743961395
1105	54-55	0.6344437984841562
1105	56-57	0.620417000543604
1105	58-59	0.5834957124761644
1105	60-61	0.5751762019194757
1105	62-63	0.5448681113555907
1105	64-65	0.550058867203461
1105	66-67	0.5569374809905199
1105	68-69	0.5967923217826012
1105	70-71	0.4840911455448378
1105	72-73	0.4677710526109635
1105	74-75	0.4841627565240856
1105	76-77	0.4722472611864248
1105	78-79	0.5767685547779848
1105	80-81	0.5811802011301523
1105	82-83	0.5304166278883713
1105	84-85	0.4985994541447525
1105	86-87	0.5969701605614013
1105	88-89	0.5988801959223942
1105	90-91	0.5909979342123108
1105	92-93	0.6130917508664133
1105	94-95	0.615418921488871
1105	96-97	0.6200103242186259
1105	98-99	0.6341816426062863
1105	100	0.7568862819436539
1106	1	0.352463830120481
1106	2	0.5131802862282981
1106	3	0.4988622588718137
1106	4	0.5912851604703135
1106	5	0.527527648230766
1106	6	0.24647363083622764
1106	7	0.25397946082471634
1106	8	0.3339978077649093
1106	9	0.47477924042936337
1106	10-11	0.39378473656022805
1106	12-13	0.32023743084916845
1106	14-15	0.4760098231124843
1106	16-17	0.6213617194737964
1106	18-19	0.5816507835126643
1106	20-21	0.4181069875095602
1106	22-23	0.7345206688838104
1106	24-25	0.5994591232507105
1106	26-27	0.4346434133492565
1106	28-29	0.5916975853289372
1106	30-31	0.6507407152859415
1106	32-33	0.5350770787646368
1106	34-35	0.443084974250759
1106	36-37	0.33964759679339096
1106	38-39	0.4607365889693682
1106	40-41	0.3978134873687864
1106	42-43	0.4207227387007677
1106	44-45	0.40279894092799395
1106	46-47	0.4220098188790118
1106	48-49	0.4135437521817238
1106	50-51	0.4513701250979807
1106	52-53	0.42031819477619337
1106	54-55	0.4188551251297028
1106	56-57	0.4041151196509887
1106	58-59	0.39015536792971517
1106	60-61	0.3938231510264103
1106	62-63	0.35931801169309097
1106	64-65	0.389987610787216
1106	66-67	0.4654401818587601
1106	68-69	0.39318265790824114
1106	70-71	0.3863923028960343
1106	72-73	0.3917845537068061
1106	74-75	0.34663673065021783
1106	76-77	0.38169004017147756
1106	78-79	0.4151578180794502
1106	80-81	0.4825575830102764
1106	82-83	0.4055362454674025
1106	84-85	0.3833749826550843
1106	86-87	0.39400119635788045
1106	88-89	0.44367741435320696
1106	90-91	0.3278013600225762
1106	92-93	0.4077880352334482
1106	94-95	0.38075893299827257
1106	96-97	0.3251890181068262
1106	98-99	0.33308840122771954
1106	100	0.4149944455001453
1107	1	0.4755102337311605
1107	2	0.6223924700834083
1107	3	0.5403008747804314
1107	4	0.7062910393459703
1107	5	0.6046827508797463
1107	6	0.33446666021448834
1107	7	0.3390322722631325
1107	8	0.4103879006140332
1107	9	0.5025430948855529
1107	10-11	0.5112479171086761
1107	12-13	0.4281943704578097
1107	14-15	0.6412123771348632
1107	16-17	0.7575871057334425
1107	18-19	0.8674796340276387
1107	20-21	0.565770047601518
1107	22-23	0.7864565354043691
1107	24-25	0.4772803975552833
1107	26-27	0.4827378021146558
1107	28-29	0.6169522050412226
1107	30-31	0.6046477093221441
1107	32-33	0.5648268716917286
1107	34-35	0.43505481384109856
1107	36-37	0.4659637964605281
1107	38-39	0.49791681377715946
1107	40-41	0.32002505571670525
1107	42-43	0.4460171997712692
1107	44-45	0.36461854943659233
1107	46-47	0.38063148508138767
1107	48-49	0.4421541751342346
1107	50-51	0.44424505075995313
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2214	98-99	-1.028834258760618
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2215	10-11	-1.4039391167777495
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2215	14-15	-3.6825854220667082
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2215	22-23	-3.210107123362498
2215	24-25	-5.563766582989754
2215	26-27	-3.62558771313509
2215	28-29	-1.8709766945624793
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2215	100	-3.1056131108256686
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2216	4	-0.7189284912673628
2216	5	-0.8922741604471014
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2216	7	-0.8942058688521186
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2216	10-11	-0.8900959417113654
2216	12-13	-1.0726919034028057
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2216	36-37	-1.324294412072085
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2216	54-55	-1.323713600058447
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2216	64-65	-1.4354490976642893
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2216	80-81	-1.3882757258293736
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2216	84-85	-1.4807572731799397
2216	86-87	-1.4543074375409937
2216	88-89	-1.3066927802402368
2216	90-91	-1.4406625114684708
2216	92-93	-1.361963644363687
2216	94-95	-1.265208948159394
2216	96-97	-1.2858057163302163
2216	98-99	-1.278095044574627
2216	100	-1.2159902571222965
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	19071.0
1	3237.0
2	466.0
3	483.0
4	494.0
5	284.0
6	234.0
7	339.0
8	568.0
9	949.0
10	1685.0
11	2803.0
12	4784.0
13	6728.0
14	8806.0
15	11241.0
16	13540.0
17	16089.0
18	18806.0
19	21242.0
20	24817.0
21	28718.0
22	33300.0
23	39047.0
24	44310.0
25	49885.0
26	56922.0
27	65500.0
28	78665.0
29	93835.0
30	112733.0
31	136177.0
32	168429.0
33	215426.0
34	299090.0
35	409377.0
36	608478.0
37	951490.0
38	833444.0
39	277235.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80165655493463	20.642979544122188	12.731676604955616	23.82368729598757
2	15.39541048980389	19.604445055800362	41.108422294962075	23.89172215943367
3	18.71291211576014	26.33708333351306	28.880465131727366	26.069539418999437
4	12.265784946320034	16.682753620750702	39.84115318877501	31.210308244154245
5	13.291401475727755	38.29297515993374	35.02018627247242	13.39543709186608
6	30.2227243403714	38.1576577655009	18.21110056404174	13.408517330085958
7	27.201933045789676	31.64109133127937	23.479145894391777	17.677829728539173
8	26.035812379786222	35.260809424328635	19.98074853758781	18.72262965829733
9	26.018158124120788	15.38107032820982	21.394093431488464	37.206678116180925
10-11	25.55074839159492	25.83864235494953	27.781125493521337	20.82948375993422
12-13	25.872322859690357	23.572364463095965	27.701103267075737	22.854209410137937
14-15	23.228747090501034	24.89835229627897	25.020676943605725	26.85222366961427
16-17	22.334788724446632	27.533876496647338	26.35614150989302	23.77519326901301
18-19	21.87102544792271	28.036940811407433	27.822576642635035	22.269457098034813
20-21	23.519850074525777	27.117457265683452	26.83124375243092	22.531448907359845
22-23	23.842359240424116	26.800825144851704	26.63132683762384	22.72548877710034
24-25	23.348623650301455	26.90002156690991	27.171598483476178	22.579756299312457
26-27	23.233424355799865	27.151636813347135	26.26762762516578	23.347311205687223
28-29	22.444910554420844	27.572759146443417	26.906779706726113	23.07555059240962
30-31	23.170955618608673	27.20420946071463	26.762063331343356	22.86277158933335
32-33	23.530697436832394	27.40208973756646	25.917568046465945	23.149644779135205
34-35	23.56771450066845	27.250348252415378	26.715089873453095	22.466847373463075
36-37	23.277932320952637	26.887163530467788	26.421843371421527	23.413060777158044
38-39	23.407140404710262	26.938325805809082	26.967016646730187	22.687517142750472
40-41	23.078554137198086	26.211459021017536	27.536053446051856	23.173933395732526
42-43	22.998732231248184	26.415632843638004	26.952770137846787	23.63286478726702
44-45	23.169922868580034	27.17758615368312	26.78814122493855	22.8643497527983
46-47	23.165384649833562	26.91496149848831	26.361446625629036	23.558207226049095
48-49	23.69741846860128	26.570944672130487	26.638824348176566	23.09281251109167
50-51	23.337642834499402	26.57838986937054	26.56850628632568	23.515461009804383
52-53	22.910024852688345	27.00723894562456	26.44737104734634	23.63536515434075
54-55	22.855837240292846	26.907662676082932	26.48600238463904	23.75049769898518
56-57	23.793541958347138	26.445554662839942	26.29221304440563	23.468690334407288
58-59	22.874229608039588	26.776063529864157	27.01269721181822	23.33700965027803
60-61	22.855463202643072	27.336495660278608	26.446973879245604	23.361067257832715
62-63	23.31030337172835	26.698041182072124	26.08670417526519	23.904951270934337
64-65	22.546577189665765	26.85106282890059	26.462418774892438	24.13994120654121
66-67	23.182201883007703	26.512853754449445	26.421653121256167	23.88329124128669
68-69	22.661477047286716	26.627277616723273	26.69160469569331	24.019640640296704
70-71	22.58652061307292	26.26983719377536	26.91703973459344	24.226602458558276
72-73	23.16525340929212	26.403378222287778	27.11517352546167	23.316194842958435
74-75	23.255907798454725	26.727934682839503	26.76206238345142	23.254095135254346
76-77	23.06697780496462	26.580736828137084	26.450811775354698	23.9014735915436
78-79	22.899287441891786	26.59660802138322	26.554153250714524	23.949951286010474
80-81	22.566332918035588	26.881484352107464	27.101695010342162	23.45048771951478
82-83	23.30272851845055	26.056352691927962	27.918653573713886	22.7222652159076
84-85	23.48021890031283	26.507032883061793	26.735400216854554	23.27734799977082
86-87	22.35494476057504	27.12058510767832	27.134925982146964	23.389544149599672
88-89	23.229107214194293	25.944681811181823	27.044288693663205	23.78192228096068
90-91	22.932221168164087	26.035720633936748	27.185324038233443	23.846734159665715
92-93	23.200052087713537	26.264676120224955	27.158121478314417	23.377150313747087
94-95	22.21368880670186	26.207012557577492	27.705424615195955	23.873874020524692
96-97	22.610308739135696	26.079311933250754	27.294204203079957	24.016175124533593
98-99	22.037158526393505	26.695038751648514	27.225124584059706	24.04267813789827
100	22.687842518741828	26.030686554363992	26.608121017104075	24.673349909790108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15946.0
1	9378.0
2	2585.0
3	1876.0
4	1005.5
5	524.0
6	413.0
7	395.5
8	416.5
9	418.5
10	396.5
11	367.0
12	367.0
13	386.0
14	377.5
15	463.5
16	564.5
17	660.0
18	800.0
19	993.0
20	1284.0
21	1710.0
22	2355.0
23	3307.0
24	4513.0
25	6198.5
26	8568.5
27	11244.5
28	15858.5
29	24412.0
30	35815.0
31	47388.5
32	62630.5
33	77161.5
34	88548.5
35	101312.5
36	113967.0
37	134790.5
38	145095.0
39	138957.0
40	132373.0
41	125666.0
42	126533.5
43	134740.0
44	145981.0
45	169212.5
46	192427.5
47	207528.0
48	217017.5
49	213098.0
50	203971.0
51	207541.0
52	208885.0
53	200665.0
54	191066.5
55	167915.5
56	148443.0
57	125881.0
58	102959.0
59	95026.5
60	77187.0
61	55578.5
62	42798.0
63	34554.0
64	25061.0
65	14044.5
66	9171.5
67	6941.0
68	5195.5
69	3957.0
70	2948.5
71	2215.0
72	1667.0
73	1347.0
74	1137.5
75	876.5
76	640.5
77	426.0
78	244.5
79	144.0
80	103.0
81	72.0
82	50.0
83	40.0
84	34.0
85	29.0
86	23.0
87	15.0
88	13.5
89	16.5
90	15.0
91	11.0
92	5.5
93	5.5
94	5.5
95	5.5
96	5.0
97	2.0
98	2.0
99	2.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.998968449493304
2	0.4478208441886531
3	0.4804458290030105
4	0.46593629628294103
5	0.4337835151961599
6	0.48609080992812626
7	0.502682331810691
8	0.5046999295557895
9	0.5186943309366847
10-11	0.5330428851132624
12-13	0.531658469639232
14-15	0.5283745073519974
16-17	0.5335150888408386
18-19	0.5330750808219608
20-21	0.5302311265536039
22-23	0.49305581490987566
24-25	0.527827180304125
26-27	0.5087673207546846
28-29	0.5141225403015149
30-31	0.517556749229342
32-33	0.5197138617121334
34-35	0.5322057966871044
36-37	0.5502675892668951
38-39	0.5392351930862506
40-41	0.5451592034867523
42-43	0.5424869596647869
44-45	0.5525856802931784
46-47	0.5498275812480173
48-49	0.5223109822138027
50-51	0.5378615095151198
52-53	0.5301238075246093
54-55	0.5404800938225879
56-57	0.5276340060519347
58-59	0.5331394722393575
60-61	0.5269257004605703
62-63	0.5204221673034978
64-65	0.5326672685117813
66-67	0.5305423517376883
68-69	0.5506324739654768
70-71	0.5346204748394829
72-73	0.5385161555919867
74-75	0.5353180485279478
76-77	0.52395296335742
78-79	0.5546247418440757
80-81	0.5275803465374374
82-83	0.528106209779511
84-85	0.537121008215057
86-87	0.8444612434497831
88-89	1.1131022368290575
90-91	1.1036045027630357
92-93	1.1031001033267611
94-95	1.1081440976895072
96-97	1.1013829988628476
98-99	1.0962209535682075
100	1.0917350181562333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4659006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.70067367848444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77814833564604	25.60151449998073
2	13.184380903513249	9.150137987713101
3	5.194507954200998	5.407587763170619
4	2.4993192804085353	3.4691225107120456
5	1.4076456516573077	2.4423126206548904
6	0.8678521745234677	1.806903306558119
7	0.5776506555034426	1.4031406827751316
8	0.37463575398209387	1.0400090437780494
9	0.2897917495887269	0.9050372043475939
>10	1.4552475059260728	9.124448120495549
>50	0.14564449087078238	3.565952832573957
>100	0.1717147710578902	13.185731197571016
>500	0.03291797605544728	8.078295722294893
>1k	0.019552782749668204	11.957889954994224
>5k	7.425107373291723E-4	1.727477651899922
>10k+	2.475035791097241E-4	1.1344389004801738
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14897	0.3197463149865014	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14272	0.3063314363621768	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12382	0.2657648434022193	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11284	0.24219758463500585	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9767	0.2096369912380452	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	8993	0.19302400554968163	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8812	0.18913905670007722	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7187	0.1542603722768333	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6583	0.14129623357428603	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6433	0.1380766627044481	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5741	0.12322370909159594	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5659	0.12146367701608456	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5486	0.11775043861287149	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5331	0.11442354871403901	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5280	0.11332889461829411	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5183	0.11124690545579895	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4941	0.10605266445246048	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4918	0.10555899691908532	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4785	0.10270431074782904	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4688	0.10062232158533388	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.585522319567736E-5	0.0	0.0	0.0	2.146380579891934E-5
7	8.585522319567736E-5	0.0	0.0	0.0	2.146380579891934E-5
8	8.585522319567736E-5	0.0	0.0	0.0	2.146380579891934E-5
9	8.585522319567736E-5	0.0	0.0	0.0	2.146380579891934E-5
10-11	9.658712609513703E-5	2.146380579891934E-5	0.0	0.0	2.146380579891934E-5
12-13	1.073190289945967E-4	4.292761159783868E-5	0.0	0.0	1.1805093189405638E-4
14-15	1.2878283479351605E-4	1.0731902899459672E-4	0.0	0.0	1.3951473769297572E-4
16-17	1.6097854349189506E-4	1.502466405924354E-4	0.0	0.0	1.6097854349189506E-4
18-19	1.9317425219027407E-4	1.502466405924354E-4	0.0	1.073190289945967E-5	2.3610186378811274E-4
20-21	1.9317425219027407E-4	1.502466405924354E-4	0.0	5.365951449729836E-5	2.7902947538595144E-4
22-23	1.9317425219027407E-4	1.502466405924354E-4	0.0	9.658712609513705E-5	3.004932811848708E-4
24-25	1.9317425219027407E-4	1.9317425219027407E-4	0.0	3.004932811848708E-4	3.219570869837901E-4
26-27	1.9317425219027407E-4	2.146380579891934E-4	0.0	5.151313391740642E-4	3.219570869837901E-4
28-29	1.9317425219027407E-4	2.2536996088865307E-4	0.0	0.0013844154740302976	3.219570869837901E-4
30-31	1.9317425219027407E-4	2.3610186378811274E-4	0.0	0.005140581488841182	3.326889898832498E-4
32-33	1.9317425219027407E-4	2.3610186378811274E-4	0.0	0.015529063495518143	3.4342089278270946E-4
34-35	1.9317425219027407E-4	3.219570869837901E-4	0.0	0.031723504970802785	4.0781231017946747E-4
36-37	1.9317425219027407E-4	4.185442130789272E-4	0.0	0.06103233178922715	4.0781231017946747E-4
38-39	1.9317425219027407E-4	5.795227565708222E-4	0.0	0.10965858382667891	4.1854421307892714E-4
40-41	1.9317425219027407E-4	6.546460768670399E-4	0.0	0.16332883022687672	4.292761159783868E-4
42-43	1.9317425219027407E-4	7.726970087610963E-4	0.0	0.22103427211727136	4.400080188778465E-4
44-45	2.146380579891934E-4	7.726970087610963E-4	0.0	0.2915965336812187	4.7220372757622554E-4
46-47	2.3610186378811274E-4	7.834289116605559E-4	0.0	0.38150841617289183	4.936675333751449E-4
48-49	2.3610186378811274E-4	8.048927174594753E-4	0.0	0.4856508019092485	4.936675333751449E-4
50-51	3.326889898832498E-4	8.156246203589349E-4	0.0	0.5994948278667167	5.365951449729836E-4
52-53	3.4342089278270946E-4	8.156246203589349E-4	0.0	0.713016896737201	5.365951449729836E-4
54-55	3.541527956821691E-4	8.370884261578543E-4	0.0	0.8418641229481139	5.365951449729836E-4
56-57	3.648846985816288E-4	8.585522319567736E-4	0.0	0.9998806612397579	5.580589507719029E-4
58-59	3.648846985816288E-4	8.585522319567736E-4	0.0	1.1913056132574202	6.224503681686609E-4
60-61	3.7561660148108846E-4	9.014798435546123E-4	0.0	1.3976586422082307	6.439141739675802E-4
62-63	5.151313391740642E-4	9.014798435546123E-4	0.0	1.6148616249903949	7.297693971632576E-4
64-65	5.151313391740642E-4	9.014798435546123E-4	0.0	1.8361105351656555	7.297693971632576E-4
66-67	5.151313391740642E-4	9.44407455152451E-4	0.0	2.0947493950426335	7.512332029621769E-4
68-69	5.151313391740642E-4	9.44407455152451E-4	0.0	2.390520638951742	7.726970087610963E-4
70-71	5.258632420735239E-4	9.551393580519106E-4	0.0	2.7190993100244985	7.726970087610963E-4
72-73	5.365951449729836E-4	9.658712609513703E-4	0.0	3.06387242257254	7.941608145600156E-4
74-75	5.365951449729836E-4	9.658712609513703E-4	0.0	3.4003605060821984	8.263565232583946E-4
76-77	5.365951449729836E-4	0.0010302626783481284	0.0	3.7867948656859425	8.370884261578543E-4
78-79	5.365951449729836E-4	0.0010302626783481284	0.0	4.212572381319106	8.47820329057314E-4
80-81	5.365951449729836E-4	0.0010302626783481284	0.0	4.674237380248062	8.585522319567736E-4
82-83	5.365951449729836E-4	0.0010302626783481284	0.0	5.154747600668469	8.585522319567736E-4
84-85	5.473270478724432E-4	0.0010302626783481284	0.0	5.627155234399783	8.80016037755693E-4
86-87	5.687908536713626E-4	0.0010302626783481284	0.0	6.1296014643466865	9.336755522529913E-4
88	5.795227565708222E-4	0.0010302626783481284	0.0	6.537531825458048	9.658712609513703E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4190	0.0	36.024597	1
GGGCACC	4505	0.0	33.116302	94
CAACGCA	8685	0.0	32.885967	5
GTATCAA	9535	0.0	32.650238	1
TAGGCAT	5130	0.0	31.958393	5
GGTATCA	6055	0.0	31.472437	1
ATCAACG	9450	0.0	30.178623	3
GCATCGT	4775	0.0	29.004333	8
AACGCAG	11035	0.0	28.076242	6
TAGTACT	5880	0.0	27.803377	4
CTGTGCG	5800	0.0	27.76719	9
CTAGGCA	6050	0.0	27.720972	4
TATCAAC	11295	0.0	27.413546	2
TATTCTC	2845	0.0	26.584011	5
TACCCCG	410	0.0	26.352442	5
ACGCAGA	10850	0.0	26.221474	7
GTACATG	18810	0.0	25.653698	1
CTAACGC	2260	0.0	25.570501	3
CTAGTAC	6770	0.0	25.122477	3
GTACTGT	6615	0.0	25.121223	6
>>END_MODULE