FastQCFastQC Report
Mon 16 Jan 2017
ERR1146470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659006
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC172330.36988576533277695No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA123920.26597948146020844No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT120790.2592613102451467No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103830.2228586956101795No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC103180.22146354823324976No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA98950.21238435838030686No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC78160.16776110612435358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77250.1658078997966519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72890.15644968046832308No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG64880.1392571720233887No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC62150.1333975530402837No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT61680.1323887541677345No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG60000.12878283479351604No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG58780.12616425048604787No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT55940.12006852963915479No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA54180.11629089981854498No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52360.11238448716314167No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT51660.11088202075721731No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC51630.11081762933982055No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA50730.10888588681791782No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT50360.1080917260033578No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT48830.10480776371612315No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA48720.10457166185233503No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG47610.10218917940865498No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA47250.10141648239989388No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT46710.10025743688675225No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84300.044.0162431
GTATCAA142100.038.026771
GGGCACC46100.035.41049694
GTGGTAT24350.032.2538151
CAACGCA163750.031.4840435
GTACTAG42900.030.8043881
ATCAACG169200.030.5935063
TATCAAC184900.029.2178822
AACGCAG187200.028.5691246
CTGTGCG60350.028.5070869
TAGGCAT55000.027.8560835
TAGTACT62050.027.8077954
TACCCCG3700.026.6736665
ACTGTGC74400.026.0878098
TCAACGC203000.025.939664
ACGCAGA199500.025.6769897
GTACTGT70050.025.493886
CGCAGAG201650.025.3799138
CTAGTAC72650.025.3683823
TATTCTC28450.024.7784485
Produced by FastQC (version 0.11.5)