##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4659006
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.859401984028352	31.0	31.0	34.0	30.0	34.0
2	31.83228654352452	31.0	31.0	34.0	30.0	34.0
3	32.032384804827466	33.0	31.0	34.0	30.0	34.0
4	35.76786271578101	37.0	35.0	37.0	35.0	37.0
5	35.66166474136329	37.0	35.0	37.0	33.0	37.0
6	35.70336355007914	37.0	35.0	37.0	33.0	37.0
7	35.628108656653374	37.0	35.0	37.0	33.0	37.0
8	35.61428403397635	37.0	35.0	37.0	33.0	37.0
9	37.215785298409145	39.0	37.0	39.0	33.0	39.0
10-11	37.16382099529385	39.0	37.0	39.0	33.0	39.0
12-13	37.09388268656447	39.0	37.0	39.0	33.0	39.0
14-15	38.31620489434871	40.0	38.0	41.0	33.0	41.0
16-17	38.31356667065893	40.0	38.0	41.0	33.0	41.0
18-19	38.36634971064643	40.0	38.0	41.0	33.5	41.0
20-21	38.38059341413168	40.0	38.0	41.0	34.0	41.0
22-23	38.35075067514401	40.0	38.0	41.0	33.5	41.0
24-25	38.28506456098146	40.0	38.0	41.0	33.0	41.0
26-27	38.146042954226715	40.0	37.0	41.0	33.0	41.0
28-29	38.08233612491591	40.0	37.0	41.0	33.0	41.0
30-31	37.96633949387487	40.0	37.0	41.0	33.0	41.0
32-33	37.85571096066414	40.0	37.0	41.0	33.0	41.0
34-35	37.76352541722419	40.0	37.0	41.0	33.0	41.0
36-37	37.68553270804974	40.0	36.0	41.0	32.0	41.0
38-39	37.57828010953409	40.0	36.0	41.0	32.0	41.0
40-41	37.45890314371778	40.0	36.0	41.0	32.0	41.0
42-43	37.342207758478956	40.0	36.0	41.0	31.0	41.0
44-45	37.16234836357798	39.0	35.5	41.0	31.0	41.0
46-47	36.95014183282872	39.0	35.0	41.0	31.0	41.0
48-49	36.71185677803377	39.0	35.0	41.0	30.0	41.0
50-51	36.52287494371117	39.0	35.0	41.0	30.0	41.0
52-53	36.33987517938375	39.0	35.0	41.0	30.0	41.0
54-55	36.13846097214727	38.0	35.0	40.0	29.5	41.0
56-57	35.89359929993651	38.0	35.0	40.0	29.0	41.0
58-59	35.62638039101044	37.5	34.0	40.0	28.5	41.0
60-61	35.37189992886894	37.0	34.0	40.0	28.0	41.0
62-63	35.094349953616714	36.5	34.0	40.0	28.0	41.0
64-65	34.819702206865585	36.0	34.0	39.0	28.0	41.0
66-67	34.59292443495458	35.5	34.0	39.0	28.0	41.0
68-69	34.39488262088523	35.0	33.0	39.0	28.0	41.0
70-71	34.16635715858705	35.0	33.0	38.0	27.5	40.5
72-73	33.94969506371102	35.0	33.0	37.0	27.0	40.0
74-75	33.755002247260464	35.0	33.0	37.0	27.0	39.5
76-77	33.584862200220385	35.0	33.0	36.5	27.0	39.0
78-79	33.45455629376738	35.0	33.0	36.0	27.0	39.0
80-81	33.29090829674827	35.0	33.0	36.0	27.0	38.0
82-83	33.09815559370389	35.0	33.0	35.5	27.0	37.0
84-85	32.947378904427254	35.0	33.0	35.0	27.0	37.0
86-87	32.804523861956824	35.0	33.0	35.0	27.0	36.5
88-89	32.689871509072965	35.0	33.0	35.0	27.0	36.0
90-91	32.594982384654585	35.0	33.0	35.0	27.0	36.0
92-93	32.519639811582124	35.0	33.0	35.0	27.0	36.0
94-95	32.47051066257481	35.0	33.0	35.0	27.0	35.0
96-97	32.41825241263909	35.0	33.0	35.0	27.0	35.0
98-99	32.377563368667055	35.0	33.0	35.0	27.0	35.0
100	31.877692151501844	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48346760386698406
1101	2	0.5894366784214959
1101	3	0.47173376365893915
1101	4	0.3191214821650519
1101	5	0.34448685252435496
1101	6	0.397737839032942
1101	7	0.3895909912631339
1101	8	0.38618585603079936
1101	9	0.4475959839862895
1101	10-11	0.49043095912364265
1101	12-13	0.4872430933057075
1101	14-15	0.6831402305351091
1101	16-17	0.6560506698642001
1101	18-19	0.6286360726778923
1101	20-21	0.6365680340208897
1101	22-23	0.6478021489538435
1101	24-25	0.6631344970499242
1101	26-27	0.7067602853879862
1101	28-29	0.727625663545119
1101	30-31	0.7635671735334739
1101	32-33	0.7256562071318129
1101	34-35	0.7695849851977243
1101	36-37	0.8162230989442278
1101	38-39	0.8024725335235345
1101	40-41	0.8270795262339448
1101	42-43	0.810400006902313
1101	44-45	0.8249960643598158
1101	46-47	0.8389931663988008
1101	48-49	0.8182366836123123
1101	50-51	0.798173793443631
1101	52-53	0.8560850701918099
1101	54-55	0.8079510828746521
1101	56-57	0.742430626238793
1101	58-59	0.8125133130186484
1101	60-61	0.8112459705671355
1101	62-63	0.8038606976704372
1101	64-65	0.8213146207568229
1101	66-67	0.7432435247346234
1101	68-69	0.715808396998618
1101	70-71	0.6904526021408586
1101	72-73	0.6381334865483126
1101	74-75	0.6323869747485347
1101	76-77	0.6442831107379661
1101	78-79	0.6233052438513553
1101	80-81	0.5845137294734926
1101	82-83	0.5881496448016321
1101	84-85	0.6060269769477244
1101	86-87	0.6146772398749718
1101	88-89	0.5944099777713561
1101	90-91	0.6144157703700657
1101	92-93	0.5765718661762946
1101	94-95	0.6014605600104517
1101	96-97	0.5851683064490558
1101	98-99	0.5909878151509034
1101	100	0.6599368425019847
1102	1	0.4197879842023582
1102	2	0.5079512998634037
1102	3	0.42017729316904706
1102	4	0.30999138060035136
1102	5	0.3372101649984529
1102	6	0.32449422089846536
1102	7	0.3447545365105853
1102	8	0.3709047714982887
1102	9	0.4506593283118008
1102	10-11	0.4383542990484841
1102	12-13	0.46146277074520015
1102	14-15	0.5342984106688178
1102	16-17	0.5798238399579176
1102	18-19	0.5540983188921373
1102	20-21	0.5815112473281445
1102	22-23	0.5674304805258501
1102	24-25	0.5956772842189793
1102	26-27	0.6638303235817844
1102	28-29	0.6229877705405116
1102	30-31	0.6567365911632663
1102	32-33	0.6771706665776591
1102	34-35	0.5977337694585927
1102	36-37	0.5891894808433591
1102	38-39	0.6353193662054437
1102	40-41	0.5865189832386548
1102	42-43	0.6154063772875986
1102	44-45	0.5930067212767156
1102	46-47	0.6715386110769472
1102	48-49	0.6830137628917186
1102	50-51	0.6386656993938757
1102	52-53	0.5683855902795756
1102	54-55	0.5448677532662458
1102	56-57	0.6005664375788555
1102	58-59	0.5772963589659668
1102	60-61	0.5600900202973804
1102	62-63	0.5529877059352941
1102	64-65	0.5930158756000381
1102	66-67	0.5350426146039453
1102	68-69	0.5386818424448947
1102	70-71	0.5178873106553823
1102	72-73	0.45139814278729773
1102	74-75	0.41779089627483046
1102	76-77	0.45321825702875174
1102	78-79	0.4065832090180308
1102	80-81	0.39386803927612846
1102	82-83	0.44126717278464156
1102	84-85	0.4997713940149566
1102	86-87	0.5095582257232749
1102	88-89	0.47658449629128796
1102	90-91	0.4638824816068592
1102	92-93	0.46093822895358016
1102	94-95	0.417220260911364
1102	96-97	0.42570312964375745
1102	98-99	0.47884473974902875
1102	100	0.5011147319856519
1103	1	0.2660245833947599
1103	2	-0.1705505278512156
1103	3	0.18997122602230831
1103	4	0.1763919355088035
1103	5	0.22853851234376066
1103	6	0.2352310752964044
1103	7	0.27299073905216886
1103	8	0.280101734804056
1103	9	0.3523050265128802
1103	10-11	0.3683839874561059
1103	12-13	0.37804136549390677
1103	14-15	0.5278569624705014
1103	16-17	0.49442506997814206
1103	18-19	0.5031651324068491
1103	20-21	0.4800660893372921
1103	22-23	0.49163634834045666
1103	24-25	0.47813087154306544
1103	26-27	0.4852140308977795
1103	28-29	0.49687284448971525
1103	30-31	0.5093693861320361
1103	32-33	0.5305484905754412
1103	34-35	0.532801074256561
1103	36-37	0.5498136823822435
1103	38-39	0.5753080606504142
1103	40-41	0.5886336656612841
1103	42-43	0.5538626304273322
1103	44-45	0.6129678716446278
1103	46-47	0.6042840991739027
1103	48-49	0.5714989091239886
1103	50-51	0.5883704764187314
1103	52-53	0.5887737946493772
1103	54-55	0.5133522853944612
1103	56-57	0.4715527390314733
1103	58-59	0.5002572063087527
1103	60-61	0.5152932937740218
1103	62-63	0.5871624960576796
1103	64-65	0.5680972947240406
1103	66-67	0.5786584492267295
1103	68-69	0.48214601350004926
1103	70-71	0.5057444096479529
1103	72-73	0.5222678651417922
1103	74-75	0.4971491474675531
1103	76-77	0.4979074650056603
1103	78-79	0.48797253387605366
1103	80-81	0.4068906191776094
1103	82-83	0.4076256968006433
1103	84-85	0.37278333280186615
1103	86-87	0.46403605102980094
1103	88-89	0.4305043426243387
1103	90-91	0.40618287513682105
1103	92-93	0.46817766007173844
1103	94-95	0.451768043744039
1103	96-97	0.43799492571691445
1103	98-99	0.4328944036528952
1103	100	0.48180438090940925
1104	1	0.33428015002416345
1104	2	0.4224619807877481
1104	3	0.33288485469172
1104	4	0.24881899526647544
1104	5	0.2440099150249111
1104	6	0.28760525736387876
1104	7	0.2770261145407815
1104	8	0.27449445088137736
1104	9	0.3429185511472497
1104	10-11	0.32392032214499267
1104	12-13	0.362028650850867
1104	14-15	0.4507637812848486
1104	16-17	0.3917088257919943
1104	18-19	0.43518637009602656
1104	20-21	0.500236649586931
1104	22-23	0.498142858923444
1104	24-25	0.46204414839645835
1104	26-27	0.5022996552897752
1104	28-29	0.5499427919119881
1104	30-31	0.5710600081391988
1104	32-33	0.567198721282125
1104	34-35	0.5617399089250767
1104	36-37	0.560105949918956
1104	38-39	0.5612307215158623
1104	40-41	0.5513909199547413
1104	42-43	0.4903875403328328
1104	44-45	0.5013749972798962
1104	46-47	0.5707366732415053
1104	48-49	0.5581649228815664
1104	50-51	0.5466101328587669
1104	52-53	0.45198686027408996
1104	54-55	0.5417124810367469
1104	56-57	0.5020027437097667
1104	58-59	0.5082860413169712
1104	60-61	0.5030662168923925
1104	62-63	0.4559840077302866
1104	64-65	0.4395928763637329
1104	66-67	0.4391484270390791
1104	68-69	0.4526053469646314
1104	70-71	0.4102359838428953
1104	72-73	0.4339827626532724
1104	74-75	0.4030567761937789
1104	76-77	0.4271122376254297
1104	78-79	0.3771262901924999
1104	80-81	0.3825285315557494
1104	82-83	0.3438988725074168
1104	84-85	0.3482017624389684
1104	86-87	0.3869821735146175
1104	88-89	0.34121170737927997
1104	90-91	0.3219700778935035
1104	92-93	0.36220017470806454
1104	94-95	0.3627528683753667
1104	96-97	0.39567502383604136
1104	98-99	0.3383076523967574
1104	100	0.418166384699326
1105	1	0.14239595377122072
1105	2	-0.36588109044560113
1105	3	0.04972644864479747
1105	4	0.09767074851818336
1105	5	0.12229570526359623
1105	6	0.20129877847385558
1105	7	0.24074996600723608
1105	8	0.21529787420040236
1105	9	0.32794444696023106
1105	10-11	0.29569559121680555
1105	12-13	0.29707548956208285
1105	14-15	0.35878021369217805
1105	16-17	0.3640790648894665
1105	18-19	0.35843920331764423
1105	20-21	0.3395677546887015
1105	22-23	0.3344609165860035
1105	24-25	0.35408653148711267
1105	26-27	0.38506408752490273
1105	28-29	0.3731451821284111
1105	30-31	0.39574505483207645
1105	32-33	0.43676622078174177
1105	34-35	0.3942221263220418
1105	36-37	0.4533286872761195
1105	38-39	0.4373621892519495
1105	40-41	0.413883886652485
1105	42-43	0.3657553826730009
1105	44-45	0.43935424034199144
1105	46-47	0.46910720065138634
1105	48-49	0.5200543895662193
1105	50-51	0.5217604634361237
1105	52-53	0.5038404146811573
1105	54-55	0.48497313182559765
1105	56-57	0.5389034459841966
1105	58-59	0.451089384060964
1105	60-61	0.40812395695476056
1105	62-63	0.47725462120224194
1105	64-65	0.4681667690179552
1105	66-67	0.45782671763970484
1105	68-69	0.49364106126562746
1105	70-71	0.4393792098954137
1105	72-73	0.42869061223663607
1105	74-75	0.3774833411295404
1105	76-77	0.37623064874484413
1105	78-79	0.3619308477062546
1105	80-81	0.3830913512868861
1105	82-83	0.35822176552760965
1105	84-85	0.32429963715186005
1105	86-87	0.39191790967431217
1105	88-89	0.3514345094064595
1105	90-91	0.3238136304187975
1105	92-93	0.32560893967749394
1105	94-95	0.3166918112425776
1105	96-97	0.3605151136958753
1105	98-99	0.3888375032501088
1105	100	0.45065424273762744
1106	1	0.22791765950091047
1106	2	0.2700536150100028
1106	3	0.22767584303092292
1106	4	0.16326623709974797
1106	5	0.16597626268274723
1106	6	0.15890829363165437
1106	7	0.20237126051929977
1106	8	0.15626830939982028
1106	9	0.26685091786558957
1106	10-11	0.22786867088375828
1106	12-13	0.2472989572718589
1106	14-15	0.29393420081638766
1106	16-17	0.262087521161007
1106	18-19	0.3025144764476835
1106	20-21	0.336172135632836
1106	22-23	0.24863149130113982
1106	24-25	0.25522981192939653
1106	26-27	0.3149974797350126
1106	28-29	0.3025987565394672
1106	30-31	0.25234530336867067
1106	32-33	0.24896068139702976
1106	34-35	0.2504336355192649
1106	36-37	0.2335320524382638
1106	38-39	0.19342684937861065
1106	40-41	0.19810593939129006
1106	42-43	0.20839209250378588
1106	44-45	0.2834007684345252
1106	46-47	0.273831253795052
1106	48-49	0.2520031190169618
1106	50-51	0.23634212103524277
1106	52-53	0.18782721587697893
1106	54-55	0.22186050012953729
1106	56-57	0.2412859324713139
1106	58-59	0.22862451474422585
1106	60-61	0.16975213691486601
1106	62-63	0.19780623554942878
1106	64-65	0.29915924296513197
1106	66-67	0.23754873722284486
1106	68-69	0.19205141476277987
1106	70-71	0.23693603922933448
1106	72-73	0.20945130393493372
1106	74-75	0.17966853713535613
1106	76-77	0.24131248631834268
1106	78-79	0.22179293491482355
1106	80-81	0.20518621507287094
1106	82-83	0.19458759340575682
1106	84-85	0.17029683658886796
1106	86-87	0.23988950374700835
1106	88-89	0.17516172541522934
1106	90-91	0.16790041219189078
1106	92-93	0.1685263434900719
1106	94-95	0.24127182453293017
1106	96-97	0.2481570041241028
1106	98-99	0.2217914568732411
1106	100	0.24994610818807672
1107	1	0.07644166998366586
1107	2	-0.4873818690149534
1107	3	-0.0015800619320032183
1107	4	0.04296574818962995
1107	5	0.0725671303450639
1107	6	0.13755274983389398
1107	7	0.17304270869378513
1107	8	0.18228005422358962
1107	9	0.213436101122646
1107	10-11	0.2013525846149733
1107	12-13	0.18076437198250517
1107	14-15	0.18086853107647016
1107	16-17	0.19764268476262714
1107	18-19	0.24099583457402218
1107	20-21	0.19345061127312135
1107	22-23	0.20859897815247308
1107	24-25	0.2164627123249261
1107	26-27	0.22912202318926944
1107	28-29	0.16008040453257877
1107	30-31	0.24399682072261442
1107	32-33	0.30528927590572863
1107	34-35	0.32328996706147706
1107	36-37	0.32208585125511746
1107	38-39	0.3507120339692875
1107	40-41	0.3685186657723989
1107	42-43	0.3714294219432972
1107	44-45	0.3736969905116325
1107	46-47	0.30214681354867423
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2216	76-77	-0.719859640401161
2216	78-79	-0.7206922677987322
2216	80-81	-0.6753933219688406
2216	82-83	-0.6406183773114691
2216	84-85	-0.6506583668361117
2216	86-87	-0.6375372458310906
2216	88-89	-0.6153198316778798
2216	90-91	-0.6358313700531966
2216	92-93	-0.6104931276118322
2216	94-95	-0.5300032824464971
2216	96-97	-0.6037114123476073
2216	98-99	-0.5880545496139682
2216	100	-0.6987335180986385
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	59.0
14	215.0
15	669.0
16	1703.0
17	3322.0
18	6023.0
19	9682.0
20	13778.0
21	20027.0
22	28341.0
23	33838.0
24	37104.0
25	39824.0
26	45110.0
27	55531.0
28	69513.0
29	87109.0
30	108281.0
31	133671.0
32	165823.0
33	210855.0
34	287722.0
35	397698.0
36	594476.0
37	1022909.0
38	941673.0
39	343226.0
40	816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32360032191732	21.182571727819724	13.873228204302004	24.620599745960952
2	14.842699067508455	20.050360510594018	40.14837268495017	24.958567736947362
3	18.016286575734465	25.98640159573155	29.848297752552146	26.149014075981842
4	12.380241629366521	17.168687115047042	38.57666596818352	31.87440528740291
5	12.906986927307726	38.39319904045443	34.992053870550635	13.707760161687208
6	30.15533785532794	38.5595339435064	17.589846417883987	13.69528178328167
7	26.386143597309886	32.234947210627546	23.47971780131604	17.89919139074653
8	26.12080774311087	35.26490414478968	19.563529216317814	19.050758895781634
9	25.911535636571404	14.889356227487148	21.209373844978952	37.98973429096249
10-11	25.601802186990103	25.903787202677997	27.44357916688667	21.050831443445233
12-13	25.939545903138995	23.67425583912105	27.84000492808981	22.546193329650144
14-15	23.206503067497835	24.738754752901702	25.51339709996659	26.541345079633878
16-17	21.906879620406908	27.37725680412485	26.800101354755917	23.915762220712324
18-19	21.52912015996545	27.234564626016795	29.154255220963442	22.08205999305431
20-21	22.919543713439058	26.350117471509993	28.55624444891846	22.174094366132486
22-23	23.20608820805699	26.048334466809052	28.31130076185237	22.434276563281593
24-25	22.794701273190032	26.117073040901857	28.889091364123594	22.199134321784516
26-27	22.69732531687859	26.358282210959196	28.01402360871178	22.930368863450433
28-29	21.977209301726592	26.830036278124563	28.573058287540302	22.619696132608542
30-31	22.755937639917185	26.448828355232855	28.348825908358993	22.44640809649097
32-33	23.100302940155046	26.624155452901327	27.682503521137342	22.593038085806285
34-35	23.054043394621683	26.50438534123562	28.330211231517577	22.111360032625115
36-37	22.870951443290693	26.108895330892466	28.041421281706867	22.978731944109967
38-39	22.983196415716144	26.163778282320305	28.594618680465317	22.258406621498235
40-41	22.588764391259826	25.55467482122202	29.037113400734192	22.81944738678396
42-43	22.737667134437753	25.67439741024526	28.310449897337914	23.277485557979073
44-45	22.923355325148755	26.422009329887103	27.982428011468542	22.6722073334956
46-47	22.998779138726157	26.318038654597135	27.33255763139176	23.350624575284943
48-49	23.64692153036701	26.11582732469526	27.373847576737177	22.863403568200553
50-51	23.167916414779935	26.253038336185696	27.104411016471218	23.47463423256315
52-53	22.70458548454327	26.64595194769013	27.023929567809102	23.625532999957503
54-55	22.71590907261602	26.566333815733767	26.932453744022983	23.78530336762723
56-57	23.58113389949178	26.13000595406484	26.78206620216089	23.50679394428249
58-59	22.613275380983044	26.44901266366173	27.541672032625026	23.3960399227302
60-61	22.720319913904337	26.991415653583434	26.903800351298297	23.38446408121393
62-63	23.157289344551177	26.313595646796763	26.535059195030016	23.994055813622047
64-65	22.381523011560837	26.42950019811093	26.975056482004963	24.213920308323278
66-67	22.898156817140823	26.178341474554873	26.908754785892096	24.014746922412204
68-69	22.53680897944527	26.118677131539147	27.17775662614217	24.166757262873418
70-71	22.414920700402686	25.783986484586634	27.39965232710958	24.401440487901098
72-73	23.013299403349126	25.865195279851537	27.525517245524046	23.59598807127529
74-75	23.033054303498606	26.285189954925947	27.117321313586608	23.56443442798884
76-77	22.810779461194365	26.200372042327352	26.73159430966058	24.257254186817708
78-79	22.57660925392846	26.219163472032044	26.932827729482195	24.27139954455731
80-81	22.268573466465746	26.603325849044314	27.394279364494032	23.73382131999591
82-83	22.967875234844744	25.7525602987296	28.181466169585327	23.098098296840323
84-85	23.21972708570879	26.155524054510735	26.92771583462696	23.69703302515352
86-87	22.048237331051713	26.721927741670697	27.342318865840365	23.887516061437225
88-89	22.8080019370327	25.65540136760004	27.20628324981883	24.33031344554843
90-91	22.4367909847062	25.69400590287094	27.429578749664607	24.43962436275825
92-93	22.75651269933708	25.97081673599679	27.160426382955155	24.11224418171097
94-95	21.74948220501301	25.844760560801568	27.751546838405357	24.65421039578007
96-97	22.03800935047451	25.731631378974978	27.37964124245702	24.850718028093493
98-99	21.47322691029954	26.34075797569073	27.286749242984833	24.899265871024898
100	22.116547126085166	25.67853279152678	26.651520372310124	25.55339971007793
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	126.0
2	120.0
3	157.5
4	119.5
5	95.0
6	183.5
7	510.0
8	898.5
9	1056.5
10	886.0
11	632.0
12	592.5
13	699.5
14	878.5
15	1203.5
16	1544.0
17	1980.5
18	2596.0
19	3274.0
20	4041.0
21	5000.0
22	6143.0
23	7254.0
24	8432.5
25	9976.0
26	12117.5
27	14409.0
28	18526.5
29	26723.0
30	37378.5
31	47909.5
32	62722.5
33	76599.0
34	86474.5
35	98671.5
36	110839.5
37	132414.5
38	143458.0
39	136434.5
40	129964.5
41	123361.0
42	123823.5
43	131496.5
44	142233.5
45	166966.5
46	191157.0
47	206532.0
48	217637.5
49	213306.5
50	203771.5
51	207381.5
52	208545.5
53	201344.5
54	194901.5
55	171081.5
56	149678.0
57	127248.0
58	103952.0
59	97718.0
60	79736.0
61	56141.5
62	42165.0
63	33850.0
64	24514.5
65	13324.5
66	8416.0
67	6454.5
68	4937.0
69	3720.5
70	2761.0
71	2076.5
72	1539.5
73	1199.5
74	990.0
75	749.0
76	505.5
77	313.0
78	169.5
79	72.0
80	42.5
81	27.5
82	15.5
83	8.5
84	3.5
85	4.0
86	2.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0143163584678792
2	0.004228369742387109
3	0.001652713046516789
4	0.02777416470380163
5	0.0028761499770551917
6	0.0
7	4.078123101794674E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008682109445662873
16-17	0.002629316210367619
18-19	0.0
20-21	8.585522319567736E-5
22-23	6.975736884648785E-4
24-25	0.0
26-27	1.1805093189405637E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.970804072800078E-4
36-37	0.0
38-39	0.0
40-41	3.219570869837901E-4
42-43	6.868417855654189E-4
44-45	0.0
46-47	0.0
48-49	4.078123101794674E-4
50-51	1.073190289945967E-5
52-53	0.0
54-55	2.897613782854111E-4
56-57	8.585522319567736E-5
58-59	1.2878283479351603E-4
60-61	5.365951449729835E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001169777416041104
70-71	6.224503681686609E-4
72-73	0.0
74-75	1.2878283479351603E-4
76-77	0.0027366352393622157
78-79	4.8293563047568515E-4
80-81	0.0029083456857535704
82-83	0.001652713046516789
84-85	0.005451806672925512
86-87	0.007125983525241221
88-89	0.007168911136839059
90-91	0.005494734284523351
92-93	0.008091854786192591
94-95	0.0014488068914270554
96-97	0.004400080188778465
98-99	0.0017922277842097648
100	0.08503959857531843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4659006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.27225660424841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.6539405306269	18.844573103661972
2	16.462736467942335	9.308774196595676
3	6.492465680972901	5.506699671802268
4	3.2872367983088298	3.7175040912286086
5	1.8527941089058666	2.619133524091322
6	1.1308218069917033	1.9182530580569581
7	0.7661036905758138	1.5161636087794794
8	0.5200240574513953	1.1761802874118612
9	0.3911562717600313	0.9952983438804658
>10	1.9480108550572324	9.940856416822461
>50	0.19585459629990662	3.9213409304000364
>100	0.2253319665512763	14.210869388531385
>500	0.0432043361640208	8.64236415697289
>1k	0.028727093691192397	14.198295200957864
>5k	0.0012127532956703914	2.1463832505398623
>10k+	3.7898540489699736E-4	1.337310770266974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	17233	0.36988576533277695	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12392	0.26597948146020844	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12079	0.2592613102451467	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10383	0.2228586956101795	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10318	0.22146354823324976	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9895	0.21238435838030686	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7816	0.16776110612435358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7725	0.1658078997966519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7289	0.15644968046832308	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6488	0.1392571720233887	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6215	0.1333975530402837	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6168	0.1323887541677345	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6000	0.12878283479351604	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5878	0.12616425048604787	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5594	0.12006852963915479	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5418	0.11629089981854498	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5236	0.11238448716314167	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5166	0.11088202075721731	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5163	0.11081762933982055	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5073	0.10888588681791782	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5036	0.1080917260033578	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4883	0.10480776371612315	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4872	0.10457166185233503	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4761	0.10218917940865498	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4725	0.10141648239989388	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4671	0.10025743688675225	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2878283479351605E-4	0.0	0.0	0.0	2.146380579891934E-5
7	1.2878283479351605E-4	0.0	0.0	0.0	2.146380579891934E-5
8	1.2878283479351605E-4	0.0	0.0	0.0	2.146380579891934E-5
9	1.2878283479351605E-4	0.0	0.0	0.0	2.146380579891934E-5
10-11	1.2878283479351605E-4	0.0	0.0	2.146380579891934E-5	2.146380579891934E-5
12-13	1.2878283479351605E-4	0.0	0.0	2.146380579891934E-5	1.502466405924354E-4
14-15	1.2878283479351605E-4	3.219570869837901E-5	0.0	2.146380579891934E-5	2.0390615508973373E-4
16-17	1.9317425219027407E-4	6.439141739675803E-5	0.0	2.146380579891934E-5	2.3610186378811274E-4
18-19	1.9317425219027407E-4	6.439141739675803E-5	0.0	2.146380579891934E-5	2.7902947538595144E-4
20-21	1.9317425219027407E-4	6.439141739675803E-5	0.0	5.365951449729836E-5	3.541527956821691E-4
22-23	1.9317425219027407E-4	6.439141739675803E-5	0.0	8.585522319567736E-5	3.648846985816288E-4
24-25	2.146380579891934E-4	7.51233202962177E-5	0.0	3.004932811848708E-4	3.648846985816288E-4
26-27	2.146380579891934E-4	8.585522319567736E-5	0.0	5.043994362746045E-4	4.292761159783868E-4
28-29	2.146380579891934E-4	8.585522319567736E-5	0.0	0.0013844154740302974	4.292761159783868E-4
30-31	2.146380579891934E-4	8.585522319567736E-5	0.0	0.005172777197539561	4.292761159783868E-4
32-33	2.146380579891934E-4	8.585522319567736E-5	0.0	0.01563638252451274	4.292761159783868E-4
34-35	2.3610186378811277E-4	8.585522319567736E-5	0.0	0.03193814302879198	4.936675333751449E-4
36-37	2.575656695870321E-4	1.6097854349189506E-4	0.0	0.06162258644869742	4.936675333751449E-4
38-39	2.575656695870321E-4	2.682975724864918E-4	0.0	0.1110966588152065	5.365951449729836E-4
40-41	2.575656695870321E-4	3.219570869837901E-4	0.0	0.16544301509807027	5.365951449729836E-4
42-43	2.575656695870321E-4	3.4342089278270946E-4	0.0	0.223921153997226	5.365951449729836E-4
44-45	2.897613782854111E-4	3.4342089278270946E-4	0.0	0.2957283162975107	5.365951449729836E-4
46-47	3.219570869837901E-4	3.4342089278270946E-4	0.0	0.38669192527333085	5.473270478724432E-4
48-49	3.219570869837901E-4	3.541527956821691E-4	0.0	0.4923689731243102	5.687908536713626E-4
50-51	3.4342089278270946E-4	3.648846985816288E-4	0.0	0.60737204459492	5.795227565708222E-4
52-53	3.648846985816288E-4	3.648846985816288E-4	0.0	0.7220746227843451	6.009865623697416E-4
54-55	3.648846985816288E-4	3.648846985816288E-4	0.0	0.8520272349939022	6.009865623697416E-4
56-57	3.648846985816288E-4	3.648846985816288E-4	0.0	1.011438920662476	6.224503681686609E-4
58-59	3.648846985816288E-4	3.7561660148108846E-4	0.0	1.2047419556875436	6.439141739675802E-4
60-61	3.648846985816288E-4	4.0781231017946747E-4	0.0	1.4127369657819715	6.546460768670399E-4
62-63	3.648846985816288E-4	4.292761159783868E-4	0.0	1.6318824229889382	6.653779797664996E-4
64-65	3.648846985816288E-4	4.400080188778465E-4	0.0	1.8555782070252755	6.653779797664996E-4
66-67	3.648846985816288E-4	4.936675333751449E-4	0.0	2.117157608296705	6.653779797664996E-4
68-69	3.648846985816288E-4	4.936675333751449E-4	0.0	2.4153971898726896	6.653779797664996E-4
70-71	3.8634850438054813E-4	4.936675333751449E-4	0.0	2.7468734747283	6.761098826659592E-4
72-73	4.829356304756852E-4	4.936675333751449E-4	0.0	3.095402753291153	7.405013000627173E-4
74-75	4.936675333751449E-4	4.936675333751449E-4	0.0	3.4363338446011875	8.263565232583946E-4
76-77	4.936675333751449E-4	5.151313391740642E-4	0.0	3.8298727239243737	8.370884261578543E-4
78-79	4.936675333751449E-4	5.151313391740642E-4	0.0	4.2645469870611885	8.370884261578543E-4
80-81	4.936675333751449E-4	5.365951449729836E-4	0.0	4.7352267844256914	8.47820329057314E-4
82-83	4.936675333751449E-4	5.580589507719029E-4	0.0	5.224623020446851	9.014798435546123E-4
84-85	4.936675333751449E-4	5.580589507719029E-4	0.0	5.705712763623829	9.014798435546123E-4
86-87	5.043994362746045E-4	5.580589507719029E-4	0.0	6.217796242374447	9.551393580519106E-4
88	5.365951449729836E-4	5.580589507719029E-4	0.0	6.632337455671875	0.001008798872549209
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8430	0.0	44.016243	1
GTATCAA	14210	0.0	38.02677	1
GGGCACC	4610	0.0	35.410496	94
GTGGTAT	2435	0.0	32.253815	1
CAACGCA	16375	0.0	31.484043	5
GTACTAG	4290	0.0	30.804388	1
ATCAACG	16920	0.0	30.593506	3
TATCAAC	18490	0.0	29.217882	2
AACGCAG	18720	0.0	28.569124	6
CTGTGCG	6035	0.0	28.507086	9
TAGGCAT	5500	0.0	27.856083	5
TAGTACT	6205	0.0	27.807795	4
TACCCCG	370	0.0	26.673666	5
ACTGTGC	7440	0.0	26.087809	8
TCAACGC	20300	0.0	25.93966	4
ACGCAGA	19950	0.0	25.676989	7
GTACTGT	7005	0.0	25.49388	6
CGCAGAG	20165	0.0	25.379913	8
CTAGTAC	7265	0.0	25.368382	3
TATTCTC	2845	0.0	24.778448	5
>>END_MODULE