##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4371208
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813092856711464	31.0	31.0	34.0	30.0	34.0
2	31.774905472354554	31.0	31.0	34.0	30.0	34.0
3	31.98305228211515	33.0	31.0	34.0	30.0	34.0
4	35.730309104485535	37.0	35.0	37.0	35.0	37.0
5	35.62161329316747	37.0	35.0	37.0	33.0	37.0
6	35.66652765093768	37.0	35.0	37.0	33.0	37.0
7	35.59049649433292	37.0	35.0	37.0	33.0	37.0
8	35.58015358683458	37.0	35.0	37.0	33.0	37.0
9	37.16817913949645	39.0	37.0	39.0	33.0	39.0
10-11	37.11517308258953	39.0	37.0	39.0	33.0	39.0
12-13	37.03383892965057	39.0	37.0	39.0	33.0	39.0
14-15	38.24204945177625	40.0	37.5	41.0	33.0	41.0
16-17	38.23511624246662	40.0	37.0	41.0	33.0	41.0
18-19	38.273275259379105	40.0	37.5	41.0	33.0	41.0
20-21	38.281848175607294	40.0	38.0	41.0	33.0	41.0
22-23	38.24303556362452	40.0	37.0	41.0	33.0	41.0
24-25	38.16854196826141	40.0	37.0	41.0	33.0	41.0
26-27	38.02932347305367	40.0	37.0	41.0	33.0	41.0
28-29	37.97035053010518	40.0	37.0	41.0	33.0	41.0
30-31	37.86361882115882	40.0	37.0	41.0	33.0	41.0
32-33	37.763480941652745	40.0	36.5	41.0	32.5	41.0
34-35	37.67885227607563	40.0	36.0	41.0	32.0	41.0
36-37	37.60640159425038	40.0	36.0	41.0	32.0	41.0
38-39	37.49369533547706	40.0	36.0	41.0	32.0	41.0
40-41	37.37124028415029	40.0	36.0	41.0	31.0	41.0
42-43	37.24661763979202	39.0	35.5	41.0	31.0	41.0
44-45	37.045275585147174	39.0	35.0	41.0	31.0	41.0
46-47	36.82570893446388	39.0	35.0	41.0	30.5	41.0
48-49	36.56953421113798	39.0	35.0	41.0	30.0	41.0
50-51	36.348915334159344	38.5	35.0	41.0	30.0	41.0
52-53	36.13074955481414	38.0	35.0	40.0	29.5	41.0
54-55	35.88979465173014	38.0	34.5	40.0	29.0	41.0
56-57	35.6039965382567	37.0	34.0	40.0	29.0	41.0
58-59	35.28892928453645	37.0	34.0	40.0	28.0	41.0
60-61	35.01191695750923	36.0	34.0	39.5	28.0	41.0
62-63	34.70590955635147	35.5	34.0	39.0	28.0	41.0
64-65	34.41154218696525	35.0	33.0	39.0	28.0	41.0
66-67	34.1781611627724	35.0	33.0	38.0	27.5	40.5
68-69	33.97180161639528	35.0	33.0	37.0	27.0	40.0
70-71	33.779439116143635	35.0	33.0	37.0	27.0	40.0
72-73	33.58676503154277	35.0	33.0	36.5	27.0	39.0
74-75	33.42159226923084	35.0	33.0	36.0	27.0	39.0
76-77	33.29255997426798	35.0	33.0	36.0	27.0	39.0
78-79	33.20185060971704	35.0	33.0	35.0	27.0	37.5
80-81	33.08508814954584	35.0	33.0	35.0	27.0	37.0
82-83	32.91964132111764	35.0	33.0	35.0	27.0	37.0
84-85	32.81649752196647	35.0	33.0	35.0	27.0	36.0
86-87	32.714654393019046	35.0	33.0	35.0	27.0	36.0
88-89	32.629054485625026	35.0	33.0	35.0	27.0	36.0
90-91	32.55419943411523	35.0	33.0	35.0	27.0	36.0
92-93	32.48683487493617	35.0	33.0	35.0	27.0	35.0
94-95	32.46444987289554	35.0	33.0	35.0	27.0	35.0
96-97	32.41920997582362	35.0	33.0	35.0	27.0	35.0
98-99	32.38737346747169	35.0	33.0	35.0	27.0	35.0
100	31.878227254342505	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4875740476183843
1101	2	0.5814810608238794
1101	3	0.47035916458362337
1101	4	0.37600479729148617
1101	5	0.3582149636791385
1101	6	0.40606081686293294
1101	7	0.42368803772440344
1101	8	0.4009679091941294
1101	9	0.44052074988950096
1101	10-11	0.5015480683536424
1101	12-13	0.5233082481174378
1101	14-15	0.6915076110489835
1101	16-17	0.6458316919669755
1101	18-19	0.6867358964055583
1101	20-21	0.6639318100617686
1101	22-23	0.6595142137809304
1101	24-25	0.7071657362918415
1101	26-27	0.7528367583875593
1101	28-29	0.7302394704063317
1101	30-31	0.7779994177358276
1101	32-33	0.7570473708140568
1101	34-35	0.7579909358743322
1101	36-37	0.7367130991690445
1101	38-39	0.7642080345755389
1101	40-41	0.6912909644101148
1101	42-43	0.7018522639411984
1101	44-45	0.7191259330380646
1101	46-47	0.7536394842561762
1101	48-49	0.7885608525384384
1101	50-51	0.77108378093385
1101	52-53	0.7685339093843311
1101	54-55	0.7467356074075084
1101	56-57	0.7341591838124586
1101	58-59	0.692708521707253
1101	60-61	0.6882884274099013
1101	62-63	0.6672779900258377
1101	64-65	0.6942620946834452
1101	66-67	0.6763301842927376
1101	68-69	0.6422919672240752
1101	70-71	0.6059495555720034
1101	72-73	0.5559302314049503
1101	74-75	0.5873181323776251
1101	76-77	0.5939437875266478
1101	78-79	0.5844678478539862
1101	80-81	0.5891512175982001
1101	82-83	0.5845379829885857
1101	84-85	0.6532701931744
1101	86-87	0.6064373656932815
1101	88-89	0.6265291264748356
1101	90-91	0.6397168113753224
1101	92-93	0.6636298484933434
1101	94-95	0.618301594455275
1101	96-97	0.6478120843251745
1101	98-99	0.6368065390620501
1101	100	0.7261999845176845
1102	1	0.41528349797323116
1102	2	0.5004942836885924
1102	3	0.4367644039423304
1102	4	0.31541302724750864
1102	5	0.2832977689659373
1102	6	0.32801217837580054
1102	7	0.31760348807355143
1102	8	0.33274904566944485
1102	9	0.3517624722739967
1102	10-11	0.4397380530679129
1102	12-13	0.43302900356371055
1102	14-15	0.5565101884506589
1102	16-17	0.5438266184734815
1102	18-19	0.5477222890021807
1102	20-21	0.5499980645628284
1102	22-23	0.553660149958219
1102	24-25	0.600551474922419
1102	26-27	0.5896551549761355
1102	28-29	0.5926770864530653
1102	30-31	0.6620456034618769
1102	32-33	0.6713449347724136
1102	34-35	0.5973611521766742
1102	36-37	0.6113505819263096
1102	38-39	0.663952126469141
1102	40-41	0.6686872229136682
1102	42-43	0.7235111775430809
1102	44-45	0.6572002216782238
1102	46-47	0.6591363133874708
1102	48-49	0.6649733847527273
1102	50-51	0.6663097377510638
1102	52-53	0.5735876968916358
1102	54-55	0.6212801168298299
1102	56-57	0.6469277750052242
1102	58-59	0.5805084478378504
1102	60-61	0.5903112993034512
1102	62-63	0.5524939005007496
1102	64-65	0.5683063801583899
1102	66-67	0.5862350737687265
1102	68-69	0.5614336178021233
1102	70-71	0.5422948444468645
1102	72-73	0.5353211925107928
1102	74-75	0.5388426102271993
1102	76-77	0.5338453685027602
1102	78-79	0.4975925257327276
1102	80-81	0.49974846008913687
1102	82-83	0.5559860412237114
1102	84-85	0.5145122543499738
1102	86-87	0.46133888711121784
1102	88-89	0.4631976349853062
1102	90-91	0.5332834795467676
1102	92-93	0.5274597987639922
1102	94-95	0.5049203157826412
1102	96-97	0.4734979139934481
1102	98-99	0.49848407624685365
1102	100	0.60883884936991
1103	1	0.2685356971530588
1103	2	-0.07806218172984813
1103	3	0.20685547057393805
1103	4	0.22079845104045148
1103	5	0.22163929902154678
1103	6	0.2602662775852167
1103	7	0.314708601866478
1103	8	0.31214409702781154
1103	9	0.38363324849908764
1103	10-11	0.4220223744365228
1103	12-13	0.45629146634324513
1103	14-15	0.5250974832795805
1103	16-17	0.47216521933142275
1103	18-19	0.47890895980143
1103	20-21	0.43393299495534166
1103	22-23	0.49072991856739634
1103	24-25	0.48468648232702805
1103	26-27	0.49828910987035613
1103	28-29	0.5910154380922705
1103	30-31	0.5710994568222816
1103	32-33	0.5768101699875174
1103	34-35	0.5583525407918728
1103	36-37	0.516542861200115
1103	38-39	0.5531088913712807
1103	40-41	0.5933345784045301
1103	42-43	0.5770793316711789
1103	44-45	0.5434873293824865
1103	46-47	0.5231069803424404
1103	48-49	0.5478015733572477
1103	50-51	0.4989766888546967
1103	52-53	0.5377429787668007
1103	54-55	0.5697450171791942
1103	56-57	0.5797067692356279
1103	58-59	0.45441004259204476
1103	60-61	0.5190358381873708
1103	62-63	0.47174527550368595
1103	64-65	0.5403512777909185
1103	66-67	0.5506863906440316
1103	68-69	0.458149827515129
1103	70-71	0.4412968023934809
1103	72-73	0.4258761271645852
1103	74-75	0.5126647270209261
1103	76-77	0.4594152720761713
1103	78-79	0.42548029516538577
1103	80-81	0.39100783255636884
1103	82-83	0.4407656827208015
1103	84-85	0.45563990067559956
1103	86-87	0.4205222991122639
1103	88-89	0.4295100629619668
1103	90-91	0.41799322571924336
1103	92-93	0.43765869812981606
1103	94-95	0.4120001198335359
1103	96-97	0.47452278216200483
1103	98-99	0.46854948387920103
1103	100	0.5476992633608404
1104	1	0.3385026880297808
1104	2	0.443274174178935
1104	3	0.32591305102057433
1104	4	0.2480652166739361
1104	5	0.2062569357737516
1104	6	0.2757332773710459
1104	7	0.23267693015772295
1104	8	0.27298655288142726
1104	9	0.27085545461115856
1104	10-11	0.31056072675441015
1104	12-13	0.3394240419248078
1104	14-15	0.4273420634840974
1104	16-17	0.38989570934291606
1104	18-19	0.44175041888293975
1104	20-21	0.435832906178689
1104	22-23	0.44201036719520204
1104	24-25	0.4208696592035963
1104	26-27	0.47065894865740177
1104	28-29	0.4574871059019756
1104	30-31	0.48406501455522744
1104	32-33	0.4720523649963937
1104	34-35	0.5563839333485419
1104	36-37	0.5316761187652546
1104	38-39	0.4989974073198127
1104	40-41	0.45814076684651894
1104	42-43	0.40889910633011084
1104	44-45	0.4562506852727495
1104	46-47	0.4430595070182406
1104	48-49	0.43354264005364485
1104	50-51	0.5156531875677715
1104	52-53	0.5079558523248835
1104	54-55	0.457075137060869
1104	56-57	0.3924065909486316
1104	58-59	0.43736215630441677
1104	60-61	0.4426739410851326
1104	62-63	0.48034929145064353
1104	64-65	0.4235572225076254
1104	66-67	0.4323963911017188
1104	68-69	0.36767987549504255
1104	70-71	0.3740980988610332
1104	72-73	0.3761296262609193
1104	74-75	0.3749750983770568
1104	76-77	0.37522637310581786
1104	78-79	0.3703798999852381
1104	80-81	0.34404935878809795
1104	82-83	0.3223709138003912
1104	84-85	0.30171892307728854
1104	86-87	0.34985332283180526
1104	88-89	0.37260159899372525
1104	90-91	0.3439861284782353
1104	92-93	0.33273113966946255
1104	94-95	0.3332225002846485
1104	96-97	0.35618133688087994
1104	98-99	0.36475425573787135
1104	100	0.43142333392238186
1105	1	0.0967395400230302
1105	2	-0.4616986486724244
1105	3	-0.03824819557364734
1105	4	0.06299628286406289
1105	5	0.11207601203918927
1105	6	0.17734871219406045
1105	7	0.19424504822475797
1105	8	0.19602251497976653
1105	9	0.3185351903562932
1105	10-11	0.2847078379442394
1105	12-13	0.24035471523654195
1105	14-15	0.36960611832831347
1105	16-17	0.3241167068483861
1105	18-19	0.3831450969630694
1105	20-21	0.3394851628165725
1105	22-23	0.403273925827186
1105	24-25	0.41660014462608785
1105	26-27	0.3704983440967595
1105	28-29	0.35870714892452327
1105	30-31	0.3734287237818563
1105	32-33	0.38580285959130123
1105	34-35	0.43855977763931264
1105	36-37	0.3891459749214121
1105	38-39	0.44958879683120045
1105	40-41	0.3962969520060682
1105	42-43	0.4050561935188668
1105	44-45	0.38888799557167886
1105	46-47	0.4138444437773998
1105	48-49	0.4700767025403607
1105	50-51	0.4230428813201996
1105	52-53	0.41503993756777646
1105	54-55	0.4406906044051908
1105	56-57	0.35989384950195813
1105	58-59	0.3470875488200065
1105	60-61	0.36828885609173767
1105	62-63	0.37261696997295957
1105	64-65	0.48154975379670617
1105	66-67	0.4967825286665004
1105	68-69	0.43955262380966076
1105	70-71	0.43302535551203647
1105	72-73	0.4284511450340176
1105	74-75	0.4031847767185823
1105	76-77	0.3830262418666024
1105	78-79	0.32848583185450053
1105	80-81	0.37515378331217875
1105	82-83	0.37362504300126176
1105	84-85	0.3439030165903887
1105	86-87	0.3269048622874138
1105	88-89	0.2795401470082055
1105	90-91	0.3041217790539079
1105	92-93	0.288248502114385
1105	94-95	0.3481748932847353
1105	96-97	0.32425155300945363
1105	98-99	0.2526467472845155
1105	100	0.3098634021332032
1106	1	0.24710703624950625
1106	2	0.25457607600143817
1106	3	0.2218589084014546
1106	4	0.1490997397386309
1106	5	0.1431305228185451
1106	6	0.17008054443022047
1106	7	0.15506118100262256
1106	8	0.19176637442847522
1106	9	0.26703870197916046
1106	10-11	0.22690909140867177
1106	12-13	0.23423290531763286
1106	14-15	0.2985998013774491
1106	16-17	0.28479126720769443
1106	18-19	0.2910024140804168
1106	20-21	0.2739903315158685
1106	22-23	0.22688265901698657
1106	24-25	0.2602034352009497
1106	26-27	0.28287424620735635
1106	28-29	0.27021167541093405
1106	30-31	0.27955828709798425
1106	32-33	0.291738631510988
1106	34-35	0.22479058438965183
1106	36-37	0.2872485083537484
1106	38-39	0.29610962417135767
1106	40-41	0.28050936792458714
1106	42-43	0.22472863011775956
1106	44-45	0.2094892073131689
1106	46-47	0.20973208281614575
1106	48-49	0.2606811262169799
1106	50-51	0.2903193483970341
1106	52-53	0.26541280153078617
1106	54-55	0.29536841048197715
1106	56-57	0.2782942849111478
1106	58-59	0.26239049986485696
1106	60-61	0.21567200399364594
1106	62-63	0.16532493254212
1106	64-65	0.187097015650302
1106	66-67	0.17685896645369326
1106	68-69	0.1915450858184471
1106	70-71	0.2834468033677595
1106	72-73	0.25608476641120603
1106	74-75	0.24627932950646425
1106	76-77	0.2737774141489169
1106	78-79	0.2766592908047727
1106	80-81	0.2390425904539768
1106	82-83	0.23014442240210542
1106	84-85	0.244120560112151
1106	86-87	0.2702635747550417
1106	88-89	0.23509069567533913
1106	90-91	0.17607616539581272
1106	92-93	0.16019170023009366
1106	94-95	0.17146053923457316
1106	96-97	0.22895481338917278
1106	98-99	0.2746795861650071
1106	100	0.29227224551615194
1107	1	0.08369233622137173
1107	2	-0.5310520947844317
1107	3	-0.05318771280631651
1107	4	0.035509306873521496
1107	5	0.0732879895434948
1107	6	0.13393253314571751
1107	7	0.1473735929081883
1107	8	0.13745135187559754
1107	9	0.1335350673491078
1107	10-11	0.16747066911383968
1107	12-13	0.09741095185044202
1107	14-15	0.21122520330558103
1107	16-17	0.2719546491080038
1107	18-19	0.20565379075662094
1107	20-21	0.152952783377458
1107	22-23	0.19572138927839688
1107	24-25	0.18323189571184173
1107	26-27	0.2530398304247612
1107	28-29	0.23174111061727842
1107	30-31	0.2377245231002263
1107	32-33	0.20994952336872785
1107	34-35	0.2607966754907878
1107	36-37	0.3060102776654148
1107	38-39	0.301240456470417
1107	40-41	0.32738628635747347
1107	42-43	0.35941024831216595
1107	44-45	0.22001184455807277
1107	46-47	0.255921741853399
1107	48-49	0.22700813452460977
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2216	80-81	-0.5346787076803707
2216	82-83	-0.5124683128908458
2216	84-85	-0.6060073727026705
2216	86-87	-0.5386361261114487
2216	88-89	-0.5590430040943843
2216	90-91	-0.6398582091319618
2216	92-93	-0.6406036524551979
2216	94-95	-0.6286875071182223
2216	96-97	-0.5852723168656837
2216	98-99	-0.5823492656862612
2216	100	-0.607614431572717
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	37.0
14	207.0
15	531.0
16	1422.0
17	2852.0
18	5267.0
19	8212.0
20	12130.0
21	17981.0
22	24931.0
23	29940.0
24	32818.0
25	36996.0
26	43744.0
27	55854.0
28	70426.0
29	87542.0
30	108628.0
31	134093.0
32	166750.0
33	210710.0
34	287549.0
35	398949.0
36	609340.0
37	1035234.0
38	757478.0
39	231226.0
40	352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.595772020213246	21.440544291650706	13.337831573619901	24.625852114516135
2	15.257752384020234	19.91746469554411	39.49493683107076	25.3298460893649
3	18.346907629584944	26.100053327183893	29.354567206778842	26.19847183645232
4	12.688709964563463	16.965830722007404	38.494819071382445	31.850640242046683
5	13.003557917739625	38.79738628481278	34.73728328089798	13.461772516549624
6	30.217322076643345	38.49187684502773	17.381648276631996	13.909152801696923
7	26.509240392030637	31.89937566186225	23.69039636584005	17.900987580267063
8	26.59322091284606	34.93265477186169	19.137890487023267	19.33623382826898
9	26.06069077472406	14.329334133722302	21.329893246901086	38.28008184465255
10-11	26.460237078629067	25.392820474340272	26.645002937403117	21.501939509627544
12-13	26.522805595158133	23.05646402550508	27.181742438245905	23.238987941090883
14-15	23.85664003997436	24.028976833084638	24.780040367877564	27.33434275906344
16-17	22.546144202348163	26.61301097211673	26.089822194972413	24.751022630562698
18-19	21.888171416230936	27.024634837783974	28.364047192446574	22.723146553538516
20-21	23.683355047406838	26.313716698255973	27.53165317115075	22.47127508318644
22-23	24.221462192659867	25.571831372571445	27.389809376197476	22.816897058571207
24-25	23.719747493141487	25.572404699112923	28.08439909517003	22.623448712575563
26-27	23.56886789744277	25.99012604895431	26.714355342133285	23.726650711469635
28-29	22.486713055063955	26.53702363282644	27.75251143391026	23.223751878199344
30-31	23.414843219540227	26.171735593456084	27.632029864513424	22.78139132249026
32-33	23.85156460182174	26.263049024434437	26.68295583280411	23.202430540939716
34-35	23.740428519562983	26.214583720813717	27.48470725013263	22.560280509490667
36-37	23.485235660256844	25.585375941844905	26.89603194357258	24.03335645432567
38-39	23.625517248321287	25.713933082113687	27.72656894844629	22.933980721118736
40-41	23.113136649735324	24.73706774573382	28.58769582513475	23.562099779396103
42-43	23.284824592769308	25.050758709737046	27.595488714580142	24.0689279829135
44-45	23.667931153127462	25.764548381134	27.306021584879968	23.261498880858564
46-47	23.53938545134434	25.891618518267716	26.434877955933462	24.134118074454474
48-49	24.519934681193345	25.633204885824778	26.586712591760204	23.260147841221677
50-51	23.79220158274344	25.45971565065259	26.648963701677395	24.09911906492657
52-53	23.34521715736245	25.891984549808654	26.40378815192505	24.35901014090384
54-55	23.285845453138514	25.882875480804923	26.243761560793978	24.58751750526259
56-57	24.519665817281588	25.271061057240214	25.952235329348234	24.257037796129964
58-59	22.941657225116487	25.97499423784242	26.99646606732602	24.08688246971507
60-61	23.202362919045065	26.459063276280776	26.231791770847547	24.106782033826608
62-63	23.861321630084863	25.732898091328533	25.58068616272664	24.825094115859965
64-65	22.815800897120532	25.925193439112647	26.314353642557574	24.944652021209244
66-67	23.60640353879294	25.663432167949914	26.20145277918598	24.528711514071166
68-69	23.00583086210397	25.453157215942074	26.70482225736436	24.836189664589593
70-71	22.830452652208184	25.16950022070803	26.69069842910102	25.309348697982763
72-73	23.49985404492305	25.20842064710716	27.04019117827383	24.251534129695955
74-75	23.653640788470707	25.627162451649387	26.605498815427175	24.11369794445273
76-77	23.330536543293338	25.609331326965346	25.877788854362738	25.18234327537858
78-79	23.21828675329762	25.783871928579565	25.886898887395777	25.11094243072704
80-81	22.614493578549606	26.369584171136793	26.740101298245527	24.275820952068074
82-83	23.659029119283737	25.078675416208736	27.85768603224251	23.40460943226502
84-85	23.74932294660501	25.55917603960226	26.400043468686818	24.291457545105914
86-87	22.16685558814637	26.360649331648084	26.905175602029207	24.567319478176337
88-89	23.350495834178417	24.858148836163263	26.725844863941738	25.065510465716578
90-91	22.781289608184967	25.13155065043217	26.69670597171318	25.39045376966968
92-93	23.109294165773626	25.31532926941989	26.69465602398447	24.88072054082201
94-95	21.934913561470562	25.176607206219153	27.50427176794343	25.384207464366852
96-97	22.313216048757806	25.0034402941666	26.9815608240005	25.7017828330751
98-99	21.524473732674615	25.771948347275238	26.950217507432317	25.75336041261783
100	22.413697175431523	24.883714913271877	26.120532597717826	26.582055313578778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	80.0
2	56.5
3	77.5
4	60.5
5	48.0
6	103.0
7	224.0
8	393.5
9	502.5
10	410.5
11	276.5
12	262.0
13	286.5
14	362.0
15	520.0
16	699.5
17	955.5
18	1319.5
19	1731.5
20	2282.0
21	2920.0
22	3536.0
23	4107.0
24	4691.5
25	5363.0
26	6470.0
27	7669.0
28	9520.5
29	13832.5
30	20802.5
31	28269.0
32	38731.5
33	50692.5
34	60195.0
35	70195.5
36	82111.5
37	104322.5
38	117605.5
39	115806.5
40	110017.5
41	102218.0
42	103170.5
43	112545.0
44	127481.0
45	158602.5
46	189522.5
47	212821.5
48	230111.0
49	226921.0
50	216262.5
51	223517.0
52	229989.5
53	221758.5
54	213280.0
55	183639.0
56	158069.5
57	130448.0
58	100741.5
59	98942.5
60	82662.0
61	54826.5
62	39582.5
63	32752.0
64	23778.5
65	10800.5
66	5553.5
67	3882.5
68	2758.5
69	1917.0
70	1480.5
71	1060.0
72	726.5
73	558.0
74	422.5
75	345.5
76	234.5
77	128.0
78	68.5
79	28.5
80	9.5
81	5.0
82	4.5
83	4.5
84	3.5
85	1.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013954952498256774
2	0.005261703400982062
3	0.0018072807333807952
4	0.026125501234441375
5	0.0025622207865651785
6	0.0
7	4.3466245486373565E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00928805035129877
16-17	0.002928252327503061
18-19	0.0
20-21	6.863091392585299E-5
22-23	7.54940053184383E-4
24-25	0.0
26-27	2.1733122743186782E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.3466245486373565E-4
36-37	0.0
38-39	0.0
40-41	5.719242827154416E-4
42-43	8.464479384188535E-4
44-45	0.0
46-47	0.0
48-49	7.320630818757652E-4
50-51	1.1438485654308831E-5
52-53	0.0
54-55	5.147318544438974E-4
56-57	1.487003135060148E-4
58-59	3.545930552835738E-4
60-61	1.0294637088877949E-4
62-63	0.0
64-65	1.1438485654308831E-5
66-67	0.0
68-69	0.0016128264772575453
70-71	0.0011095331084679566
72-73	0.0
74-75	2.7452365570341196E-4
76-77	0.002871059899231517
78-79	5.032933687895886E-4
80-81	0.003545930552835738
82-83	0.0019216655899238835
84-85	0.005616296456265636
86-87	0.007881116615818785
88-89	0.007812485701892932
90-91	0.006131028310709534
92-93	0.008693249097274713
94-95	0.0015327570776773834
96-97	0.004929987317007107
98-99	0.0021275583317014427
100	0.08485068658366292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4371208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.80776316506728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.70336548573273	12.35733337203517
2	15.58937358300938	5.864024924819934
3	6.588789298001238	3.7176116598401157
4	3.454110265732566	2.5985635129570284
5	2.032819888437748	1.9116397509487828
6	1.3378527306478862	1.5097210384658395
7	0.8893072355587266	1.170811590715846
8	0.6090013102298649	0.9163161928015171
9	0.43532923581344163	0.7368812249408041
>10	2.4961011614462523	8.669788974997507
>50	0.31094698907725016	4.165328531050198
>100	0.3978179763530239	16.81684152779032
>500	0.08312171537400642	10.869739230259585
>1k	0.06744525150961045	22.237985299223883
>5k	0.004131781173561721	5.026574435329636
>10k+	4.860919027719672E-4	1.4308387338238373
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	24539	0.5613779989421689	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17225	0.39405583079093925	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	10455	0.23917873503159764	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10384	0.23755447006868582	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9826	0.22478912007847718	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9700	0.22190662169359132	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9006	0.20603000360541068	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8429	0.1928299911603383	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8410	0.19239532870547457	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7790	0.1782116064941316	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7719	0.17658734153121974	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7596	0.17377347406025978	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7267	0.16624695049972457	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7226	0.16530899467607124	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7032	0.1608708622421994	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6856	0.1568445152918827	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	6788	0.15528888124289672	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6538	0.14956963841574228	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6278	0.1436216258755017	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6265	0.14332422524848967	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6155	0.14080775840454174	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5853	0.13389891306933918	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5746	0.13145107713931709	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5566	0.12733322230376592	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5555	0.1270815756193711	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5494	0.12568608036954546	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5426	0.12413044632055945	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5375	0.12296372078381994	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5355	0.12250618135764758	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5334	0.12202576496016662	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5298	0.12120219399305639	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5217	0.11934915931705835	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5195	0.11884586594826875	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5174	0.11836544955078779	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5157	0.11797654103854129	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5120	0.11713009310012244	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	5109	0.11687844641572764	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5071	0.11600912150600018	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	4964	0.11356128557597808	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	4842	0.11077029507632673	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	4655	0.10649230144161523	No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC	4602	0.10527982196225849	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2876971308617663E-5
2	0.0	0.0	0.0	0.0	2.2876971308617663E-5
3	0.0	0.0	0.0	2.2876971308617663E-5	2.2876971308617663E-5
4	0.0	0.0	0.0	2.2876971308617663E-5	2.2876971308617663E-5
5	0.0	0.0	0.0	2.2876971308617663E-5	2.2876971308617663E-5
6	0.0	0.0	0.0	2.2876971308617663E-5	4.5753942617235325E-5
7	0.0	0.0	0.0	2.2876971308617663E-5	4.5753942617235325E-5
8	0.0	0.0	0.0	2.2876971308617663E-5	4.5753942617235325E-5
9	0.0	0.0	0.0	6.863091392585299E-5	4.5753942617235325E-5
10-11	0.0	0.0	0.0	6.863091392585299E-5	4.5753942617235325E-5
12-13	0.0	0.0	0.0	6.863091392585299E-5	1.6013879916032365E-4
14-15	0.0	0.0	0.0	1.0294637088877949E-4	1.6013879916032365E-4
16-17	0.0	0.0	0.0	1.1438485654308832E-4	1.830157704689413E-4
18-19	0.0	0.0	0.0	1.1438485654308832E-4	1.830157704689413E-4
20-21	0.0	0.0	0.0	1.6013879916032365E-4	2.6308517004910314E-4
22-23	0.0	0.0	0.0	2.2876971308617665E-4	2.974006270120296E-4
24-25	0.0	0.0	0.0	3.2027759832064726E-4	2.974006270120296E-4
26-27	0.0	0.0	0.0	4.8041639748097094E-4	3.202775983206473E-4
28-29	0.0	0.0	0.0	0.0013611797928627508	3.4315456962926496E-4
30-31	0.0	0.0	0.0	0.005753558284117342	3.4315456962926496E-4
32-33	0.0	0.0	0.0	0.016448542370896102	3.660315409378826E-4
34-35	0.0	0.0	0.0	0.0334232550818904	3.660315409378826E-4
36-37	0.0	0.0	0.0	0.06431860483417856	3.660315409378826E-4
38-39	0.0	0.0	0.0	0.11783927921068958	4.003469979008091E-4
40-41	0.0	0.0	0.0	0.17634713333247926	4.1178548355511795E-4
42-43	0.0	0.0	0.0	0.23954476657253557	4.1178548355511795E-4
44-45	0.0	0.0	0.0	0.3178755163332424	4.346624548637356E-4
46-47	0.0	0.0	0.0	0.416601086015582	4.689779118266621E-4
48-49	0.0	0.0	0.0	0.5389700055453779	5.376088257525152E-4
50-51	0.0	0.0	0.0	0.6699978587154856	5.948012540240592E-4
52-53	0.0	0.0	0.0	0.7956725005993767	6.176782253326769E-4
54-55	0.0	0.0	0.0	0.9348674325266608	6.176782253326769E-4
56-57	0.0	0.0	0.0	1.1130104081068666	6.176782253326769E-4
58-59	0.0	0.0	0.0	1.3292321024302665	6.176782253326769E-4
60-61	0.0	0.0	0.0	1.551813594777462	6.176782253326769E-4
62-63	0.0	0.0	0.0	1.7918616547187871	7.091861105671476E-4
64-65	0.0	0.0	0.0	2.0426847681464713	7.320630818757652E-4
66-67	0.0	0.0	0.0	2.327468745481798	7.435015675300741E-4
68-69	0.0	0.0	0.0	2.6498624636484926	7.549400531843828E-4
70-71	0.0	0.0	0.0	3.016557436754325	7.778170244930006E-4
72-73	0.0	0.0	0.0	3.3984770342660426	8.121324814559271E-4
74-75	0.0	0.0	0.0	3.772275306963201	8.464479384188535E-4
76-77	0.0	0.0	0.0	4.212931528309794	8.464479384188535E-4
78-79	0.0	0.0	0.0	4.6817836167942595	8.578864240731624E-4
80-81	0.0	0.0	0.0	5.190052726843472	8.922018810360888E-4
82-83	0.0	0.0	0.0	5.724538846012361	9.379558236533242E-4
84-85	0.0	0.0	0.0	6.243262731949612	9.49394309307633E-4
86-87	0.0	0.0	0.0	6.793145052809201	9.837097662705595E-4
88	0.0	0.0	0.0	7.238410983874481	0.0010065867375791773
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5420	0.0	43.13791	94
GGTATCA	8795	0.0	37.74566	1
GTATCAA	13815	0.0	33.764393	1
GTACTAG	5355	0.0	33.63082	1
GTGGTAT	2035	0.0	31.88694	1
TATTCTC	2090	0.0	31.705343	5
TAGGCAT	6810	0.0	30.778496	5
CTGTGCG	8095	0.0	30.602556	9
CAACGCA	14200	0.0	30.514215	5
TAGTACT	8100	0.0	30.409218	4
ACTGTGC	8670	0.0	29.378855	8
ATCAACG	14965	0.0	28.96164	3
TACTGTG	9405	0.0	28.382078	7
CTAGGCA	7620	0.0	28.31501	4
GTACTGT	9020	0.0	27.92627	6
CTAGTAC	9625	0.0	27.34993	3
TACCCCG	345	0.0	27.24398	5
TATCAAC	17255	0.0	27.000227	2
AACGCAG	17405	0.0	26.164017	6
CTAACGC	2770	0.0	25.96454	3
>>END_MODULE