##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146468_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4731312 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.53066168538452 31.0 31.0 34.0 27.0 34.0 2 30.630057370978705 31.0 31.0 34.0 27.0 34.0 3 30.812345074685414 31.0 31.0 34.0 27.0 34.0 4 34.29500506413443 37.0 35.0 37.0 30.0 37.0 5 34.509332506501366 37.0 35.0 37.0 30.0 37.0 6 34.70658476972138 37.0 35.0 37.0 31.0 37.0 7 34.6042679070837 37.0 35.0 37.0 30.0 37.0 8 34.55179324466448 37.0 35.0 37.0 30.0 37.0 9 35.94396691657621 39.0 35.0 39.0 30.0 39.0 10-11 35.856116231607636 39.0 35.0 39.0 30.0 39.0 12-13 35.82852208858769 39.0 35.0 39.0 30.0 39.0 14-15 36.84869820464176 40.0 36.0 41.0 29.5 41.0 16-17 36.69215114116338 40.0 36.0 41.0 29.0 41.0 18-19 36.64772234424616 40.0 36.0 41.0 28.5 41.0 20-21 36.980805535547006 40.0 36.0 41.0 30.0 41.0 22-23 36.698622496254735 40.0 36.0 41.0 29.0 41.0 24-25 36.80743649964323 40.0 36.0 41.0 30.0 41.0 26-27 36.87354205345156 40.0 36.0 41.0 30.0 41.0 28-29 36.71616879208135 40.0 35.5 41.0 29.5 41.0 30-31 36.66701519578502 40.0 35.0 41.0 29.5 41.0 32-33 36.77031793718106 40.0 35.5 41.0 30.0 41.0 34-35 36.820183809480326 40.0 35.0 41.0 30.0 41.0 36-37 36.755655302377015 40.0 35.0 41.0 30.0 41.0 38-39 36.5800196647357 39.0 35.0 41.0 30.0 41.0 40-41 36.53778708738717 39.0 35.0 41.0 29.5 41.0 42-43 36.37767737574694 39.0 35.0 41.0 29.0 41.0 44-45 36.17875126392003 39.0 35.0 41.0 28.0 41.0 46-47 36.03727317073995 39.0 35.0 41.0 28.0 41.0 48-49 35.89382279164849 38.0 34.5 40.5 28.0 41.0 50-51 35.65624281383261 38.0 34.0 40.0 27.5 41.0 52-53 35.44906370579662 38.0 34.0 40.0 27.0 41.0 54-55 35.2436585243163 37.5 34.0 40.0 27.0 41.0 56-57 35.07629934360702 37.0 33.5 40.0 27.0 41.0 58-59 34.7874046353316 36.5 33.0 40.0 26.5 41.0 60-61 34.53233891571725 36.0 33.0 39.5 26.0 41.0 62-63 34.256187395800566 35.5 33.0 39.0 26.0 41.0 64-65 33.96349997632792 35.0 33.0 39.0 26.0 41.0 66-67 33.68143593151329 35.0 32.0 38.0 26.0 40.5 68-69 33.40031549388415 35.0 32.0 37.5 25.5 40.0 70-71 33.36275952632166 35.0 32.0 37.0 26.0 40.0 72-73 33.17653517671208 35.0 32.0 37.0 26.0 40.0 74-75 32.925879544616805 35.0 32.0 36.0 25.0 39.0 76-77 32.711030893756316 35.0 32.0 36.0 25.0 39.0 78-79 32.50578444203215 35.0 32.0 36.0 25.0 39.0 80-81 32.31232964556132 35.0 32.0 35.0 24.5 37.5 82-83 32.07665579864528 35.0 31.0 35.0 24.0 37.0 84-85 31.90980556767341 35.0 31.0 35.0 24.0 37.0 86-87 31.75112970778507 35.0 31.0 35.0 24.0 36.0 88-89 31.76980592275462 35.0 32.0 35.0 24.0 36.0 90-91 31.69535934218669 35.0 32.0 35.0 24.0 36.0 92-93 31.5483674507198 35.0 32.0 35.0 24.0 36.0 94-95 31.545638609332887 35.0 32.0 35.0 24.0 35.0 96-97 31.45618403521053 35.0 31.5 35.0 24.0 35.0 98-99 31.379730611720383 35.0 31.0 35.0 23.5 35.0 100 30.892138375148374 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6442800556151305 1101 2 0.8305929278776105 1101 3 0.8726431364535898 1101 4 0.9085858576529944 1101 5 0.8442026599375012 1101 6 0.5473890840378886 1101 7 0.6165239850064523 1101 8 0.6139031019739747 1101 9 0.8412227648912776 1101 10-11 0.8142329023892003 1101 12-13 0.6349474389887746 1101 14-15 0.8723198720832386 1101 16-17 1.011195806942311 1101 18-19 1.1650215198747773 1101 20-21 0.8417597688161109 1101 22-23 1.098183010815987 1101 24-25 0.9929743406867146 1101 26-27 0.9184996195568189 1101 28-29 1.1009137433686647 1101 30-31 1.1322698414213548 1101 32-33 0.9824608372484178 1101 34-35 0.8673687101943983 1101 36-37 0.8598493139513366 1101 38-39 0.9482787387877991 1101 40-41 0.891539062985423 1101 42-43 0.853806473101244 1101 44-45 0.8468607936968837 1101 46-47 0.9076157046172781 1101 48-49 0.8968887868100026 1101 50-51 0.8297784196763587 1101 52-53 0.8825349920087859 1101 54-55 0.8248611727726498 1101 56-57 0.7687800726404888 1101 58-59 0.6779280918538362 1101 60-61 0.7382159404259099 1101 62-63 0.6843426296087856 1101 64-65 0.6833110387678261 1101 66-67 0.6919681011260934 1101 68-69 0.7127255359234752 1101 70-71 0.6575671606125795 1101 72-73 0.7181059898302351 1101 74-75 0.6375956936901943 1101 76-77 0.6502790767317421 1101 78-79 0.6457055752347785 1101 80-81 0.675215273743504 1101 82-83 0.7166490062465556 1101 84-85 0.6888971008821088 1101 86-87 0.6697972000601631 1101 88-89 0.6888861453174826 1101 90-91 0.6782197403457744 1101 92-93 0.7299279877400444 1101 94-95 0.7492196696946074 1101 96-97 0.6788640035273161 1101 98-99 0.66317214658174 1101 100 0.7170076992092014 1102 1 0.7149973384547899 1102 2 0.8436701396447859 1102 3 0.8429666356802272 1102 4 0.9559329374405507 1102 5 0.7603985590716249 1102 6 0.6151652761852091 1102 7 0.6799529030508111 1102 8 0.7168048300592815 1102 9 0.8243604180875721 1102 10-11 0.826844873424875 1102 12-13 0.7850087230789384 1102 14-15 0.9825637696207252 1102 16-17 1.1640620994592865 1102 18-19 1.1451730770551265 1102 20-21 0.9042293385890403 1102 22-23 1.1939385947710548 1102 24-25 1.0571831044460964 1102 26-27 0.8971651268889076 1102 28-29 1.0758856056753103 1102 30-31 1.114167797282434 1102 32-33 1.0249724720611795 1102 34-35 0.8900610379711793 1102 36-37 0.9114160102654765 1102 38-39 0.9537645082870014 1102 40-41 0.8804579934214658 1102 42-43 0.8463840145360209 1102 44-45 0.9306782664753399 1102 46-47 0.8646729793238919 1102 48-49 0.7758005371437875 1102 50-51 0.8637852683242571 1102 52-53 0.8282743260160714 1102 54-55 0.9200213127612002 1102 56-57 0.7855249445024057 1102 58-59 0.7917203493550602 1102 60-61 0.7996159973487593 1102 62-63 0.7352216330508625 1102 64-65 0.6603202060976656 1102 66-67 0.748342566782739 1102 68-69 0.7979971561639303 1102 70-71 0.7410996733228998 1102 72-73 0.7136449391952198 1102 74-75 0.784894467443543 1102 76-77 0.6878803569617276 1102 78-79 0.6602908640021923 1102 80-81 0.736280360413673 1102 82-83 0.6837414978830552 1102 84-85 0.6879256199055206 1102 86-87 0.7085208563837178 1102 88-89 0.7222941013518636 1102 90-91 0.7177152499768411 1102 92-93 0.747754870195056 1102 94-95 0.6957887731451358 1102 96-97 0.6689929863831736 1102 98-99 0.7367587229336898 1102 100 0.8009636173929238 1103 1 0.6365887810979558 1103 2 0.7849714334861311 1103 3 0.7469278964316146 1103 4 0.7821673819541743 1103 5 0.7361145549902304 1103 6 0.5367838113671226 1103 7 0.6043067703829621 1103 8 0.619131975761043 1103 9 0.7014134044616469 1103 10-11 0.8806505090942451 1103 12-13 0.749502790495626 1103 14-15 0.9155487268445484 1103 16-17 1.112588450649426 1103 18-19 1.1672459476752053 1103 20-21 0.856003849163983 1103 22-23 0.9938764633290589 1103 24-25 0.9398610402045193 1103 26-27 0.8401959935801955 1103 28-29 1.071011240545758 1103 30-31 1.0625992270616251 1103 32-33 0.8805654077782492 1103 34-35 0.890404161366007 1103 36-37 0.8952841588959544 1103 38-39 0.9470951405585879 1103 40-41 0.8051292764694864 1103 42-43 0.8446626728245548 1103 44-45 0.8891704100682105 1103 46-47 0.892606781387407 1103 48-49 0.9185007227228041 1103 50-51 0.9303930647962062 1103 52-53 0.9080972445796078 1103 54-55 0.9148513873439015 1103 56-57 0.7950790824556648 1103 58-59 0.8023823848945426 1103 60-61 0.7459855074329553 1103 62-63 0.7847085054382603 1103 64-65 0.7747013450250932 1103 66-67 0.7878182292177414 1103 68-69 0.7874696470749569 1103 70-71 0.7467158064167521 1103 72-73 0.6871849107740005 1103 74-75 0.7064553163883431 1103 76-77 0.7839247721849816 1103 78-79 0.7436826052601404 1103 80-81 0.7427478311884528 1103 82-83 0.6783201326406854 1103 84-85 0.6261512571789964 1103 86-87 0.6854852936523805 1103 88-89 0.7298794172972833 1103 90-91 0.7320706285616225 1103 92-93 0.8293804763540358 1103 94-95 0.7276799486000876 1103 96-97 0.744380491608581 1103 98-99 0.7592760457742322 1103 100 0.8260551702787318 1104 1 0.5198672235409347 1104 2 0.7060117678884765 1104 3 0.7160065616387925 1104 4 0.8008326851503256 1104 5 0.6795449315769417 1104 6 0.43135969522247564 1104 7 0.40687319956250434 1104 8 0.41626852765293876 1104 9 0.6169142398273806 1104 10-11 0.6265949044622872 1104 12-13 0.4937496490723703 1104 14-15 0.7166388631275922 1104 16-17 0.8983255408881234 1104 18-19 0.8318242000004687 1104 20-21 0.7398043951923157 1104 22-23 0.9966682172130277 1104 24-25 0.7975824121247115 1104 26-27 0.6783646862488268 1104 28-29 0.896623442006522 1104 30-31 0.9276900610830978 1104 32-33 0.7471265335056003 1104 34-35 0.6761252484296847 1104 36-37 0.6128805300159925 1104 38-39 0.7126102187501004 1104 40-41 0.6161089748416444 1104 42-43 0.5834692520498734 1104 44-45 0.6363946593592757 1104 46-47 0.5606349181697468 1104 48-49 0.6156542379802303 1104 50-51 0.6568723982959739 1104 52-53 0.7063520183267116 1104 54-55 0.6657215599861601 1104 56-57 0.6004592698848441 1104 58-59 0.5746900107275081 1104 60-61 0.5933640273313117 1104 62-63 0.5284808698753594 1104 64-65 0.5211458396128137 1104 66-67 0.6177164810308966 1104 68-69 0.5766918287557985 1104 70-71 0.5017376027411373 1104 72-73 0.4698745964076281 1104 74-75 0.538088712454126 1104 76-77 0.5569365377192597 1104 78-79 0.4938006286759773 1104 80-81 0.5326573492368283 1104 82-83 0.48797076732660827 1104 84-85 0.4521271307923129 1104 86-87 0.4536729020738939 1104 88-89 0.3662604752991925 1104 90-91 0.4013202076781184 1104 92-93 0.4383660991015894 1104 94-95 0.38285953623027424 1104 96-97 0.41495713401443624 1104 98-99 0.4534885775405364 1104 100 0.5258804156074035 1105 1 0.5630660588069105 1105 2 0.6420541817369845 1105 3 0.5664644312639631 1105 4 0.699700486662806 1105 5 0.5575886976965734 1105 6 0.31160720195153857 1105 7 0.495633038435777 1105 8 0.382132350274091 1105 9 0.5108876316819959 1105 10-11 0.5628513792269487 1105 12-13 0.545246824838749 1105 14-15 0.6955642677801421 1105 16-17 0.7939503761556779 1105 18-19 0.794936216045592 1105 20-21 0.5873660722017107 1105 22-23 0.8654121799874943 1105 24-25 0.6596087357666889 1105 26-27 0.5101307046274144 1105 28-29 0.7936213113763273 1105 30-31 0.8031811692657982 1105 32-33 0.6706134372686634 1105 34-35 0.5633650029839004 1105 36-37 0.5454694739320871 1105 38-39 0.6523961196405423 1105 40-41 0.5812675019887124 1105 42-43 0.5846005911858327 1105 44-45 0.6680329788450621 1105 46-47 0.5686027265982219 1105 48-49 0.6005498612147449 1105 50-51 0.6609352402228836 1105 52-53 0.6231704663243249 1105 54-55 0.5718788719464598 1105 56-57 0.5201920898089796 1105 58-59 0.4873461267288093 1105 60-61 0.5556555404988757 1105 62-63 0.5296014905196529 1105 64-65 0.47253333291352817 1105 66-67 0.5375337476915831 1105 68-69 0.5056421770702713 1105 70-71 0.4704075077590133 1105 72-73 0.4553406961493991 1105 74-75 0.4938063359722378 1105 76-77 0.5184679928133846 1105 78-79 0.5451832429567318 1105 80-81 0.6336333760243349 1105 82-83 0.5704469957715261 1105 84-85 0.5294416051220949 1105 86-87 0.6259038232804137 1105 88-89 0.6040914955539378 1105 90-91 0.6587989814137742 1105 92-93 0.6350255487129353 1105 94-95 0.529588759945991 1105 96-97 0.5438669729831886 1105 98-99 0.5987272007462572 1105 100 0.6875807791524302 1106 1 0.573971988195634 1106 2 0.5472871115179778 1106 3 0.650017203152693 1106 4 0.7617112433154745 1106 5 0.6268760405907017 1106 6 0.4145617115752174 1106 7 0.42342262684325505 1106 8 0.45584657839495435 1106 9 0.6188801844937899 1106 10-11 0.593025570203217 1106 12-13 0.48656571613150135 1106 14-15 0.7118430909072444 1106 16-17 0.8614974372247133 1106 18-19 0.833271429272358 1106 20-21 0.6508437129305165 1106 22-23 0.9574093623295923 1106 24-25 0.737077735572349 1106 26-27 0.5702068766361066 1106 28-29 0.7996607203193378 1106 30-31 0.7593855698646124 1106 32-33 0.6939557323000471 1106 34-35 0.7095815296918317 1106 36-37 0.5666647145277253 1106 38-39 0.6977491433329064 1106 40-41 0.6522973826168723 1106 42-43 0.6475305892998975 1106 44-45 0.66813383178674 1106 46-47 0.6333980943866138 1106 48-49 0.6471612493340828 1106 50-51 0.6772186027912568 1106 52-53 0.6984381501781769 1106 54-55 0.6336256688365935 1106 56-57 0.5931605512626916 1106 58-59 0.5958458539111504 1106 60-61 0.5566761965772358 1106 62-63 0.5520306028165294 1106 64-65 0.5525994824251939 1106 66-67 0.6154856685446504 1106 68-69 0.628385814638186 1106 70-71 0.6199377621406441 1106 72-73 0.6073657648409068 1106 74-75 0.5801158193880624 1106 76-77 0.5840100848792602 1106 78-79 0.5324517491768788 1106 80-81 0.6151142051167255 1106 82-83 0.6091967898162522 1106 84-85 0.5761765065583049 1106 86-87 0.6114754163740379 1106 88-89 0.589197901806827 1106 90-91 0.5506276593275601 1106 92-93 0.6012657515996302 1106 94-95 0.6019867618773098 1106 96-97 0.6450386976156324 1106 98-99 0.6282065533508465 1106 100 0.5687518894410211 1107 1 0.48012171969486417 1107 2 0.6000482071822155 1107 3 0.5204050356713985 1107 4 0.6448437678946988 1107 5 0.5583157817193438 1107 6 0.380551164380158 1107 7 0.39017376261197967 1107 8 0.47320285379574045 1107 9 0.5737797128827964 1107 10-11 0.5538091858387446 1107 12-13 0.4357143044875116 1107 14-15 0.6081128318495175 1107 16-17 0.7735127574015124 1107 18-19 0.8704542917178273 1107 20-21 0.6075711733016078 1107 22-23 0.7958906820904588 1107 24-25 0.6232774300162589 1107 26-27 0.5378811214728998 1107 28-29 0.6398126649603881 1107 30-31 0.6524648294630424 1107 32-33 0.6258352739042223 1107 34-35 0.5109695245140102 1107 36-37 0.4488232612831027 1107 38-39 0.5444933388597022 1107 40-41 0.4054084715752637 1107 42-43 0.5471693601799856 1107 44-45 0.4621787998038016 1107 46-47 0.4459880209359355 1107 48-49 0.45241307156318555 1107 50-51 0.5134190018985834 1107 52-53 0.5051497708609887 1107 54-55 0.47049426987174314 1107 56-57 0.3356686723930622 1107 58-59 0.375955590414506 1107 60-61 0.46161940209164243 1107 62-63 0.43815713417342295 1107 64-65 0.45663806820506636 1107 66-67 0.47227064348790293 1107 68-69 0.4563495666045725 1107 70-71 0.38141643818541837 1107 72-73 0.31940733396063337 1107 74-75 0.38855664529199885 1107 76-77 0.32380179782698093 1107 78-79 0.30462460216951825 1107 80-81 0.38907835313534633 1107 82-83 0.3535560146259158 1107 84-85 0.32711342721392356 1107 86-87 0.47844857745327474 1107 88-89 0.5066377634024377 1107 90-91 0.4924292865847022 1107 92-93 0.5536230520409369 1107 94-95 0.5095515831071573 1107 96-97 0.46869823063196137 1107 98-99 0.5034254030582979 1107 100 0.5026584696913865 1108 1 0.39586212000184773 1108 2 0.464427630478653 1108 3 0.4240193575222513 1108 4 0.5786041917407445 1108 5 0.44777287994764947 1108 6 0.30660293628397284 1108 7 0.31097942026865866 1108 8 0.29951366825585524 1108 9 0.41187361713321735 1108 10-11 0.4620355692781217 1108 12-13 0.23513005297699863 1108 14-15 0.4273136671588418 1108 16-17 0.6208274685390052 1108 18-19 0.6666276247045104 1108 20-21 0.3801808368277122 1108 22-23 0.5811440401409698 1108 24-25 0.4568249755283489 1108 26-27 0.45425860673680063 1108 28-29 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2215 92-93 -4.085754630979615 2215 94-95 -2.0410425227142746 2215 96-97 -1.674274879544278 2215 98-99 -3.085804320463435 2215 100 -3.1852369983039104 2216 1 -0.6145443784207458 2216 2 -0.591323461195497 2216 3 -0.7802675266659485 2216 4 -0.6843009463284844 2216 5 -0.909839066337085 2216 6 -0.8006610000553565 2216 7 -0.9936587309798313 2216 8 -0.7716848314003784 2216 9 -0.9940702931203518 2216 10-11 -0.9602704936151198 2216 12-13 -1.1842972643078582 2216 14-15 -1.5726808852018408 2216 16-17 -1.2658305861062047 2216 18-19 -3.1831036272734323 2216 20-21 -1.4833158132918953 2216 22-23 -0.9804550718659755 2216 24-25 -1.1613213289602484 2216 26-27 -1.150432487373962 2216 28-29 -1.0380747693221224 2216 30-31 -1.2279257677093511 2216 32-33 -1.126020233219954 2216 34-35 -1.3730343484653744 2216 36-37 -1.3646780583107088 2216 38-39 -1.4435627688989072 2216 40-41 -1.427942341806343 2216 42-43 -1.4781722648891673 2216 44-45 -1.2761334655764998 2216 46-47 -1.5132083273521246 2216 48-49 -1.4417913085573488 2216 50-51 -1.362092614142874 2216 52-53 -1.3106539208053434 2216 54-55 -1.20368599577305 2216 56-57 -1.3777129488244881 2216 58-59 -1.404676321321439 2216 60-61 -1.379372215076522 2216 62-63 -1.3678049157428092 2216 64-65 -1.350493122254143 2216 66-67 -1.3651641834006867 2216 68-69 -1.4037017208559206 2216 70-71 -1.513339337572134 2216 72-73 -1.5128644610125832 2216 74-75 -1.5252863443196603 2216 76-77 -1.4027174031570162 2216 78-79 -1.4851149890610529 2216 80-81 -1.453163511999783 2216 82-83 -1.6078249401522982 2216 84-85 -1.4820444999280369 2216 86-87 -1.4845543010611912 2216 88-89 -1.2985830325810284 2216 90-91 -1.4544693982564212 2216 92-93 -1.4264480697806157 2216 94-95 -1.2916386883170397 2216 96-97 -1.3062905471770492 2216 98-99 -1.2939116164676143 2216 100 -1.3679297409250175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19566.0 1 3245.0 2 543.0 3 453.0 4 461.0 5 262.0 6 241.0 7 332.0 8 568.0 9 998.0 10 1743.0 11 2974.0 12 4903.0 13 6971.0 14 9454.0 15 11937.0 16 14663.0 17 17316.0 18 20219.0 19 23188.0 20 26691.0 21 30748.0 22 35853.0 23 41310.0 24 46988.0 25 53449.0 26 60620.0 27 69862.0 28 83040.0 29 99647.0 30 118919.0 31 143168.0 32 177672.0 33 226984.0 34 319109.0 35 433593.0 36 638831.0 37 977782.0 38 734243.0 39 272556.0 40 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02812693069502 21.950136992618155 12.579794944445085 23.441941132241748 2 15.345417703545037 19.798469273474957 41.23517983138611 23.620933191593892 3 18.300528804321164 27.555910560094226 28.977240749641027 25.166319885943583 4 11.98787212387116 17.05685377258024 40.91684467115198 30.038429432396622 5 12.91436572574157 38.96202179099142 35.05043990834588 13.073172574921127 6 28.98518033778541 39.16190204320733 18.49672173940563 13.356195879601634 7 26.44393545621363 31.988954360580347 24.31986553873515 17.24724464447087 8 26.168523495806944 35.0256664743416 19.994931285567038 18.810878744284416 9 26.12963854541097 14.827091523084421 22.287900762207236 36.755369169297374 10-11 25.83657256815674 25.87929487475756 27.39106528990554 20.89306726718016 12-13 25.95311614675197 23.863498606535614 27.463323216271558 22.720062030440857 14-15 23.387433154341625 24.83182209253923 24.96623055188957 26.814514201229578 16-17 22.01094861583648 27.334841626074724 26.522482125413543 24.131727632675258 18-19 21.35797887412757 28.28412395542273 28.199912856493388 22.157984313956316 20-21 23.370102104151716 27.333413796671834 26.796129770082384 22.500354329094062 22-23 24.0699646886295 26.917411241522636 26.53129933268647 22.481324737161394 24-25 23.364265254173993 27.086210082007856 27.298834058211142 22.250690605607012 26-27 23.139426005700958 27.320533173008236 26.19798485373964 23.342055967551165 28-29 22.053227051884868 27.93501886124622 26.990995190331756 23.02075889653716 30-31 23.081772117124864 27.36754816862399 26.83690267990207 22.713777034349075 32-33 23.658299787309016 27.739117474241187 25.602030428164234 23.00055231028556 34-35 23.60324205810299 27.401576660377565 26.81952468145608 22.175656600063366 36-37 23.16584169463212 27.02217329300924 26.467617810894733 23.344367201463907 38-39 23.39125841837285 26.932077864217778 27.215999219670508 22.46066449773886 40-41 22.83440754732379 25.9891496169553 28.02748554018994 23.148957295530977 42-43 22.850032780126636 26.303528034180584 27.142678024776888 23.70376116091589 44-45 23.039227615299218 27.483772187149885 26.845509563897092 22.63149063365381 46-47 22.9847572574115 27.068428692571395 26.394322014626404 23.552492035390706 48-49 23.86794897278076 26.466564049031664 26.722934616475175 22.942552361712394 50-51 23.391702885326968 26.405531055608595 26.546017488021413 23.65674857104303 52-53 22.597050639287392 27.196947136171197 26.435027358417095 23.770974866124316 54-55 22.70567460701578 27.048915127564143 26.37594283433157 23.869467431088502 56-57 23.9788170846591 26.287207074089103 26.153108672295144 23.580867168956658 58-59 22.68852063982222 26.794279216147764 27.2574468544008 23.259753289629213 60-61 22.70746603077907 27.60200859170694 26.315026775379508 23.37549860213449 62-63 23.29061567857479 26.709989858841794 25.814835581718548 24.184558880864866 64-65 22.21775512211935 26.955631850414292 26.410201761109352 24.416411266357006 66-67 23.184154904063607 26.410178987341286 26.393721578032874 24.011944530562225 68-69 22.40995008316992 26.573196278569768 26.712724939488876 24.304128698771436 70-71 22.415943571769862 26.17943350131851 26.96094655368299 24.443676373228634 72-73 23.219022144567596 26.333922437008532 27.317147910853063 23.129907507570813 74-75 23.431045787432726 26.792775951628876 26.7600189593179 23.016159301620498 76-77 22.986788247243915 26.770465277092402 26.30628348925576 23.936462986407918 78-79 22.809621698640946 26.63529174721872 26.540170183752203 24.01491637038813 80-81 22.314379113368027 27.009394258732396 27.345544747058977 23.3306818808406 82-83 23.36377515407719 25.837605139553933 28.454479101704226 22.344140604664652 84-85 23.685379295279066 26.53107671671973 26.700378213636515 23.083165774364687 86-87 22.08371230683542 27.34239729240796 27.318960796572284 23.254929604184333 88-89 23.333309110712054 25.737493326311622 27.16387800177053 23.765319561205793 90-91 22.934461685854664 25.7960295306715 27.442301102276517 23.827207681197315 92-93 23.216562663653946 26.197293745404597 27.322415465740768 23.263728125200686 94-95 21.832500528150334 26.17010811239979 28.091592220711593 23.905799138738278 96-97 22.32673842314754 26.105295955681417 27.445491577270246 24.122474043900795 98-99 21.585073301324815 27.004065315151678 27.356115538376457 24.054745845147053 100 22.545426572261025 26.063756930043024 26.426507087367956 24.964309410327996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16402.0 1 9654.5 2 2624.0 3 1832.5 4 970.5 5 531.5 6 424.5 7 402.0 8 408.0 9 404.5 10 372.5 11 330.0 12 320.5 13 310.5 14 316.0 15 478.0 16 561.5 17 611.0 18 785.5 19 947.5 20 1125.5 21 1358.0 22 1823.5 23 2507.0 24 3342.5 25 4424.5 26 6361.0 27 8536.5 28 12624.5 29 22878.5 30 37193.0 31 50673.5 32 68631.0 33 85430.5 34 98034.5 35 111599.0 36 124080.5 37 150559.0 38 161041.0 39 144385.5 40 129174.0 41 115951.5 42 113038.5 43 121427.5 44 134551.5 45 164995.5 46 195556.0 47 216809.5 48 230523.0 49 224750.0 50 212937.5 51 218296.5 52 222019.5 53 213226.5 54 202453.0 55 174164.5 56 150853.5 57 123473.0 58 96152.0 59 92089.0 60 76092.5 61 51780.0 62 38543.0 63 32116.0 64 23130.0 65 10510.5 66 5228.0 67 3546.5 68 2640.5 69 2000.0 70 1519.5 71 1097.0 72 826.0 73 733.5 74 575.5 75 417.0 76 295.5 77 181.0 78 131.5 79 95.0 80 56.0 81 44.0 82 29.5 83 23.0 84 19.5 85 12.0 86 16.0 87 18.5 88 16.0 89 11.0 90 6.5 91 5.5 92 9.0 93 8.5 94 4.5 95 5.5 96 5.0 97 4.0 98 5.5 99 4.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9958759853503637 2 0.4493679554423805 3 0.48115617824400503 4 0.4683901632359058 5 0.4345094975769934 6 0.4898218506832777 7 0.5058639125891508 8 0.5073434176397583 9 0.522434369155955 10-11 0.5355702604267061 12-13 0.5333721386372321 14-15 0.5312479921002884 16-17 0.535348334669115 18-19 0.5343443848133457 20-21 0.5333615707440135 22-23 0.49626826554663905 24-25 0.5308569800511993 26-27 0.5116868217526133 28-29 0.5165269168467436 30-31 0.5197606921716429 32-33 0.5224449370491737 34-35 0.534502903211625 36-37 0.5516862975851096 38-39 0.5412346511918893 40-41 0.546486894121546 42-43 0.5435490198067682 44-45 0.5536624936169925 46-47 0.5521090133138545 48-49 0.5250869103538299 50-51 0.5393747019854113 52-53 0.5320617198781227 54-55 0.5415094164155736 56-57 0.5298001907293368 58-59 0.5357604825046414 60-61 0.5290181666311585 62-63 0.5230895785355097 64-65 0.534756532648872 66-67 0.533150212879641 68-69 0.5534511357526201 70-71 0.5377049748568684 72-73 0.5405583060258973 74-75 0.5378317895754919 76-77 0.5254039471503887 78-79 0.5554484675709401 80-81 0.5302229064580819 82-83 0.5298107586225554 84-85 0.5390893688685083 86-87 0.8435503724971002 88-89 1.1094174300912727 90-91 1.099938029874166 92-93 1.0999168940877289 94-95 1.1042708660938023 96-97 1.0973805997152588 98-99 1.093227417680339 100 1.0886409520234557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4731312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.511995584506362 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33180796403863 15.775135291262673 2 12.128295622412997 5.218076837538725 3 5.0790459567877875 3.277812425877713 4 2.5753753481284956 2.216058524695469 5 1.5537543933791678 1.6712178824890007 6 0.9820835435152568 1.2675946111029939 7 0.6909975791097783 1.0405315809499895 8 0.5055007714369342 0.869946429049271 9 0.3775266229076771 0.7309215940521194 >10 2.0505774173186007 8.30352480700986 >50 0.26488516009037466 4.053090609260708 >100 0.33164423322971487 16.103104418652475 >500 0.0665158055496043 9.94037272467511 >1k 0.058053735612818305 22.404145569020383 >5k 0.003148677185779976 4.49489245401168 >10k+ 7.87169296444994E-4 2.633574240351862 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 20003 0.4227791361043195 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 17870 0.3776965036336644 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 17456 0.3689462880486427 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 15104 0.31923491834822987 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14625 0.3091108766447869 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 13988 0.2956473806842584 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 13790 0.29146249496968285 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 11583 0.24481581430267121 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 9130 0.19296973017209604 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8973 0.1896514117014477 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 8572 0.18117596134011033 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 8559 0.18090119611642605 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 8465 0.17891443219132452 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 7999 0.16906515571156583 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 7874 0.16642318240690954 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 7317 0.15465054936136108 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 7170 0.15154358875508528 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 7167 0.15148018139577352 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 7074 0.14951455325710922 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6936 0.14659781472876868 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6908 0.14600601270852567 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6645 0.1404473008755288 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 6577 0.13901006739779578 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 6489 0.13715011819131775 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 6303 0.13321886191398918 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5998 0.12677244705062782 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5985 0.12649768182694357 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5938 0.1255042998643928 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 5892 0.12453205368827928 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 5856 0.12377116537653826 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5794 0.12246074661742876 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 5713 0.12074874791601145 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5649 0.11939605758402744 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 5607 0.1185083545536629 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5590 0.11814904618422965 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 5476 0.11573956653038311 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5294 0.11189285339880353 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 5276 0.11151240924293304 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5115 0.10810954762653573 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 5013 0.1059536974099362 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4998 0.10563666061337743 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 4964 0.10491804387451091 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4954 0.10470668601013841 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 4923 0.10405147663058364 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 4826 0.10200130534617036 No Hit ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT 4806 0.10157858961742534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2271572874500776E-5 2 0.0 0.0 0.0 0.0 4.2271572874500776E-5 3 0.0 2.1135786437250388E-5 0.0 0.0 4.2271572874500776E-5 4 0.0 2.1135786437250388E-5 0.0 0.0 4.2271572874500776E-5 5 0.0 2.1135786437250388E-5 0.0 0.0 4.2271572874500776E-5 6 0.0 2.1135786437250388E-5 0.0 0.0 8.454314574900155E-5 7 0.0 2.1135786437250388E-5 0.0 0.0 1.0567893218625194E-4 8 0.0 2.1135786437250388E-5 0.0 0.0 1.0567893218625194E-4 9 0.0 2.1135786437250388E-5 0.0 0.0 1.0567893218625194E-4 10-11 0.0 2.1135786437250388E-5 0.0 0.0 1.0567893218625194E-4 12-13 0.0 2.1135786437250388E-5 0.0 0.0 2.1135786437250387E-4 14-15 0.0 2.1135786437250388E-5 0.0 0.0 2.4306154402837943E-4 16-17 0.0 2.1135786437250388E-5 0.0 0.0 2.536294372470046E-4 18-19 0.0 2.1135786437250388E-5 0.0 0.0 2.7476522368425504E-4 20-21 0.0 2.1135786437250388E-5 0.0 0.0 2.7476522368425504E-4 22-23 0.0 2.1135786437250388E-5 0.0 3.1703679655875584E-5 3.27604689777381E-4 24-25 0.0 2.1135786437250388E-5 0.0 1.373826118421275E-4 3.8044415587050695E-4 26-27 0.0 2.1135786437250388E-5 0.0 4.544194084008833E-4 3.8044415587050695E-4 28-29 0.0 2.1135786437250388E-5 0.0 0.0013738261184212753 3.8044415587050695E-4 30-31 0.0 2.1135786437250388E-5 0.0 0.006034267027834985 3.8044415587050695E-4 32-33 0.0 2.1135786437250388E-5 0.0 0.01710941912095419 3.8044415587050695E-4 34-35 0.0 2.1135786437250388E-5 0.0 0.034483035572374005 4.015799423077573E-4 36-37 0.0 2.1135786437250388E-5 0.0 0.06634523362652897 4.015799423077573E-4 38-39 0.0 2.1135786437250388E-5 0.0 0.11759951573686116 4.438515151822581E-4 40-41 0.0 2.1135786437250388E-5 0.0 0.1734508313972953 4.649873016195085E-4 42-43 0.0 4.2271572874500776E-5 0.0 0.23395202007392452 4.8612308805675886E-4 44-45 0.0 4.2271572874500776E-5 0.0 0.3076313715941794 5.283946609312597E-4 46-47 0.0 4.2271572874500776E-5 0.0 0.40194981857040923 5.600983405871353E-4 48-49 0.0 4.2271572874500776E-5 0.0 0.5158400037875329 6.340735931175116E-4 50-51 0.0 4.2271572874500776E-5 0.0 0.6381633677931196 6.55209379554762E-4 52-53 0.0 4.2271572874500776E-5 0.0 0.7590600662141918 6.55209379554762E-4 54-55 0.0 4.2271572874500776E-5 0.0 0.8944030746651246 6.763451659920123E-4 56-57 0.0 4.2271572874500776E-5 0.0 1.06562408059329 6.974809524292627E-4 58-59 0.0 4.2271572874500776E-5 0.0 1.2757032298863402 7.397525253037636E-4 60-61 0.0 4.2271572874500776E-5 0.0 1.4914996094106665 7.608883117410139E-4 62-63 0.0 4.2271572874500776E-5 0.0 1.717166401201189 8.665672439272658E-4 64-65 0.0 4.2271572874500776E-5 0.0 1.9567933799335153 8.877030303645162E-4 66-67 0.0 4.2271572874500776E-5 0.0 2.2341054658834594 9.088388168017667E-4 68-69 0.0 4.2271572874500776E-5 0.0 2.5459956984447443 9.088388168017667E-4 70-71 0.0 4.2271572874500776E-5 0.0 2.8951377546016834 9.29974603239017E-4 72-73 0.0 4.2271572874500776E-5 0.0 3.259962564295062 9.511103896762674E-4 74-75 0.0 4.2271572874500776E-5 0.0 3.6120107910871235 9.82814069332143E-4 76-77 0.0 4.2271572874500776E-5 0.0 4.0214215422698825 0.0010145177489880187 78-79 0.0 4.2271572874500776E-5 0.0 4.467016759833213 0.0010356535354252689 80-81 0.0 4.2271572874500776E-5 0.0 4.953139847889972 0.0010462214286438942 82-83 0.0 4.2271572874500776E-5 0.0 5.461740844822747 0.0011413324676115208 84-85 0.0 4.2271572874500776E-5 0.0 5.958305011379508 0.0011413324676115208 86-87 0.0 4.2271572874500776E-5 0.0 6.488453942585059 0.0011730361472673963 88 0.0 4.2271572874500776E-5 0.0 6.914995248675209 0.001204739826923272 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5145 0.0 39.546738 94 GTACTAG 5995 0.0 36.0338 1 TATTCTC 2860 0.0 34.171925 5 TAGGCAT 7250 0.0 33.052498 5 GCATCGT 6625 0.0 31.252048 8 CAACGCA 10285 0.0 31.065386 5 GTATCAA 11690 0.0 30.059065 1 CTAGGCA 8390 0.0 29.289804 4 ACGTTAA 880 0.0 28.809578 8 ATCAACG 11100 0.0 28.61666 3 AGGGCTA 6810 0.0 27.94345 5 TAGTACT 8170 0.0 27.375473 4 GGTATCA 7880 0.0 27.234468 1 CTGTGCG 8170 0.0 27.182987 9 TCAAGAC 1040 0.0 26.657164 3 TACCTGG 1605 0.0 26.64338 2 TCTCTGC 3825 0.0 26.144115 8 ACTGTGC 8720 0.0 26.112658 8 GCTATTG 7305 0.0 25.83638 8 GAGTTGC 7140 0.0 25.786074 94 >>END_MODULE