FastQCFastQC Report
Mon 16 Jan 2017
ERR1146467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723183
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114880.3085531922551215No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC85000.22829928048124412No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT71530.192120559209687No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA67370.18094732383554607No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60000.16115243328087822No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57270.15381999756659825No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC52110.13996088830444273No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC46590.12513486444260194No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG41130.11046999301404202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67200.032.780591
CAACGCA62150.031.9732725
GGTATCA41800.031.2588231
GTACTAG27750.031.1069371
ATCAACG66950.029.8966943
AACGCAG76750.027.2800526
GGGCACC31950.027.19269494
TATCAAC79500.027.128962
TAGGCAT35200.026.8250945
GCATCGT29650.026.6009278
CTGTGCG36800.026.1556289
TAACGCC14750.026.1179474
CTAACGC15000.025.6854233
GTACATG132500.025.6321931
ACGCAGA76600.025.6188567
TAGTACT36950.025.5563494
TACATGG134700.024.9068162
TCTAACG15550.024.7782752
ACATGGG132850.024.5095773
CGCAGAG79800.024.3562078
Produced by FastQC (version 0.11.5)