##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146467_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3723183 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.530809256488332 31.0 30.0 34.0 27.0 34.0 2 30.51140516058437 31.0 30.0 34.0 26.0 34.0 3 30.7617702379926 31.0 31.0 34.0 27.0 34.0 4 34.22492421135357 37.0 35.0 37.0 30.0 37.0 5 34.430231874178624 37.0 35.0 37.0 30.0 37.0 6 34.58752685538154 37.0 35.0 37.0 30.0 37.0 7 34.45141912175684 37.0 35.0 37.0 30.0 37.0 8 34.38667666886103 37.0 35.0 37.0 30.0 37.0 9 35.77538197826967 39.0 35.0 39.0 30.0 39.0 10-11 35.71964257464648 39.0 35.0 39.0 29.5 39.0 12-13 35.725419352204824 39.0 35.0 39.0 30.0 39.0 14-15 36.76200565483889 40.0 36.0 41.0 29.5 41.0 16-17 36.58595561915705 40.0 36.0 41.0 28.0 41.0 18-19 36.533458871078864 40.0 36.0 41.0 27.5 41.0 20-21 36.8654848552972 40.0 36.0 41.0 30.0 41.0 22-23 36.59363010091097 40.0 36.0 41.0 28.0 41.0 24-25 36.712537095275735 40.0 36.0 41.0 29.5 41.0 26-27 36.79260635321981 40.0 36.0 41.0 30.0 41.0 28-29 36.62409328254883 40.0 36.0 41.0 29.5 41.0 30-31 36.56737984676015 40.0 35.5 41.0 29.0 41.0 32-33 36.67231693956488 40.0 35.5 41.0 30.0 41.0 34-35 36.71705956972838 40.0 35.0 41.0 30.0 41.0 36-37 36.64627564640256 40.0 35.0 41.0 30.0 41.0 38-39 36.48447914056333 39.0 35.0 41.0 29.0 41.0 40-41 36.45490887769954 39.0 35.0 41.0 29.0 41.0 42-43 36.294458128971904 39.0 35.0 41.0 28.0 41.0 44-45 36.12828499163216 39.0 35.0 41.0 28.0 41.0 46-47 36.00766696130703 39.0 35.0 41.0 28.0 41.0 48-49 35.89459287926486 39.0 34.5 40.5 28.0 41.0 50-51 35.69403236424318 38.0 34.0 40.0 27.0 41.0 52-53 35.52701747402693 38.0 34.0 40.0 27.0 41.0 54-55 35.34225500062715 38.0 34.0 40.0 27.0 41.0 56-57 35.222113310036065 38.0 33.5 40.0 27.0 41.0 58-59 34.98973781304868 37.0 33.0 40.0 26.5 41.0 60-61 34.74049126782111 37.0 33.0 40.0 26.0 41.0 62-63 34.48720570544074 36.0 33.0 40.0 26.0 41.0 64-65 34.20622730604432 36.0 33.0 39.0 26.0 41.0 66-67 33.91798563218622 35.0 32.0 39.0 26.0 41.0 68-69 33.63575360115256 35.0 32.0 38.5 26.0 40.5 70-71 33.52871212615657 35.0 32.0 38.0 26.0 40.0 72-73 33.30190511720751 35.0 32.0 37.0 25.5 40.0 74-75 33.02836336543221 35.0 32.0 37.0 25.0 39.5 76-77 32.77831038656977 35.0 32.0 36.0 25.0 39.0 78-79 32.55154756024616 35.0 32.0 36.0 25.0 39.0 80-81 32.298908353416955 35.0 31.0 36.0 24.5 38.0 82-83 32.071618021461745 35.0 31.0 35.0 24.0 37.0 84-85 31.87270273849016 35.0 31.0 35.0 24.0 37.0 86-87 31.69993645222381 35.0 31.0 35.0 24.0 36.0 88-89 31.683611442145068 35.0 31.0 35.0 24.0 36.0 90-91 31.60415241474835 35.0 31.0 35.0 24.0 36.0 92-93 31.467178083913684 35.0 31.0 35.0 23.5 36.0 94-95 31.43698939858718 35.0 31.0 35.0 24.0 35.0 96-97 31.352451383668225 35.0 31.0 35.0 23.0 35.0 98-99 31.26892258586269 35.0 31.0 35.0 23.0 35.0 100 30.791260864695612 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7150320126260823 1101 2 0.8450958367132522 1101 3 0.8957820706625625 1101 4 0.8819095874069731 1101 5 0.8722932163025661 1101 6 0.6191068523191916 1101 7 0.630767733424122 1101 8 0.6659768872491796 1101 9 0.8652243343867738 1101 10-11 0.8810842910946235 1101 12-13 0.7821379164126512 1101 14-15 1.0182579869100934 1101 16-17 1.1625994424327004 1101 18-19 1.2205539521728284 1101 20-21 0.9538391960703549 1101 22-23 1.2622650895565073 1101 24-25 1.0355598495928504 1101 26-27 1.065430187720949 1101 28-29 1.2335226756443518 1101 30-31 1.17958924887958 1101 32-33 0.9816786065268062 1101 34-35 0.8640582470481206 1101 36-37 0.9144540680458206 1101 38-39 0.9886504659842643 1101 40-41 0.915867918700151 1101 42-43 0.9804678507084859 1101 44-45 0.9638255206129145 1101 46-47 0.8944250121286714 1101 48-49 0.9181384040139804 1101 50-51 0.9194414411297771 1101 52-53 0.9799687696747625 1101 54-55 1.0242966658596373 1101 56-57 0.8459836562062719 1101 58-59 0.7663899361429927 1101 60-61 0.744982302138844 1101 62-63 0.7124316766549015 1101 64-65 0.7174616629632808 1101 66-67 0.7341028167229098 1101 68-69 0.6892430409769759 1101 70-71 0.6463443853803952 1101 72-73 0.6604176387577425 1101 74-75 0.6636866133981627 1101 76-77 0.6624864584597887 1101 78-79 0.5528681229968626 1101 80-81 0.6942900957263731 1101 82-83 0.6895568171849291 1101 84-85 0.7204697098154185 1101 86-87 0.6679682811393484 1101 88-89 0.6102481522319358 1101 90-91 0.584374130564008 1101 92-93 0.6448977995852978 1101 94-95 0.6216338480812382 1101 96-97 0.5354567176927496 1101 98-99 0.5913343822828878 1101 100 0.6546298959398129 1102 1 0.8143964381080941 1102 2 0.9921449026222398 1102 3 0.8723376703826062 1102 4 0.9837358630182678 1102 5 0.826800113884552 1102 6 0.6718195346351195 1102 7 0.7592338944815182 1102 8 0.7676378592839228 1102 9 0.9361419635509165 1102 10-11 0.8861143337237252 1102 12-13 0.852816244928853 1102 14-15 1.0525048986160996 1102 16-17 1.2435092529468434 1102 18-19 1.2865771040103269 1102 20-21 0.9868603361333186 1102 22-23 1.3067707963949715 1102 24-25 1.2130054785697766 1102 26-27 1.0613640319746054 1102 28-29 1.1770513755949281 1102 30-31 1.2400434011308121 1102 32-33 1.1405125018053965 1102 34-35 0.9952235429854852 1102 36-37 0.9418606613697946 1102 38-39 1.042187256200478 1102 40-41 0.9686397289504356 1102 42-43 1.0484018761200602 1102 44-45 1.0893301315307937 1102 46-47 1.019615097600294 1102 48-49 0.9156468459248543 1102 50-51 1.0345862346946149 1102 52-53 0.9714355522954392 1102 54-55 1.0136996546986552 1102 56-57 0.8812249206109897 1102 58-59 0.8472968364896403 1102 60-61 0.902453602430775 1102 62-63 0.8337401898163677 1102 64-65 0.7800609421147868 1102 66-67 0.9012940461328682 1102 68-69 0.8614538242873664 1102 70-71 0.8022160939203289 1102 72-73 0.7372002254795191 1102 74-75 0.7416470752562958 1102 76-77 0.8067332301139771 1102 78-79 0.7077063886194921 1102 80-81 0.7570526170857903 1102 82-83 0.7612378499742718 1102 84-85 0.7462199328070085 1102 86-87 0.7983013752730805 1102 88-89 0.8309503744358331 1102 90-91 0.7701871428444278 1102 92-93 0.8353364776271697 1102 94-95 0.7950220063429327 1102 96-97 0.7796445372352032 1102 98-99 0.7807738118755054 1102 100 0.7844253366289031 1103 1 0.7378900307436993 1103 2 0.7962191675071857 1103 3 0.779039582889677 1103 4 0.7540986979762394 1103 5 0.6987147384706347 1103 6 0.5764768197133563 1103 7 0.6771087941845337 1103 8 0.6379482172095479 1103 9 0.7531813420637405 1103 10-11 0.8480134856502559 1103 12-13 0.7133506786911212 1103 14-15 0.903103988157163 1103 16-17 1.1545454627055491 1103 18-19 1.224692408432908 1103 20-21 0.9296884770662359 1103 22-23 1.1855251417891708 1103 24-25 1.1260774927758135 1103 26-27 0.9579512602752871 1103 28-29 1.1423299113608039 1103 30-31 1.14706597292502 1103 32-33 0.9892818990241494 1103 34-35 0.9033453491268091 1103 36-37 0.8528164031114827 1103 38-39 0.9309153000138863 1103 40-41 0.8191905801286339 1103 42-43 0.8829037542326645 1103 44-45 0.9484304518019968 1103 46-47 0.9254086674841204 1103 48-49 0.9563326759861042 1103 50-51 0.9246849635546255 1103 52-53 0.9446178119295254 1103 54-55 0.9215222374564007 1103 56-57 0.8027189709501314 1103 58-59 0.8225151128077144 1103 60-61 0.8650597753403559 1103 62-63 0.7567897227016545 1103 64-65 0.8007827602383912 1103 66-67 0.814328100294702 1103 68-69 0.795794984666486 1103 70-71 0.702191286485693 1103 72-73 0.7007277784077033 1103 74-75 0.7689104216682594 1103 76-77 0.7477261908907025 1103 78-79 0.7153740446728634 1103 80-81 0.7309484164006221 1103 82-83 0.6412747112389141 1103 84-85 0.6646687204626431 1103 86-87 0.7610311002434678 1103 88-89 0.765818761019954 1103 90-91 0.8021014132984661 1103 92-93 0.8959636709415442 1103 94-95 0.8207035831813485 1103 96-97 0.7693273489271881 1103 98-99 0.8706551618753622 1103 100 0.9154196884653949 1104 1 0.5232566710333231 1104 2 0.7095344631945437 1104 3 0.708006650036026 1104 4 0.7531817106714911 1104 5 0.740063767159036 1104 6 0.4374355971368473 1104 7 0.4661767579950862 1104 8 0.5056403645288512 1104 9 0.6653491946226424 1104 10-11 0.5958980385068458 1104 12-13 0.5402532795821102 1104 14-15 0.6886381268440473 1104 16-17 0.8926957048573101 1104 18-19 0.8281243255137056 1104 20-21 0.700716466463355 1104 22-23 0.9380126019474702 1104 24-25 0.7613980478710474 1104 26-27 0.6397538296069669 1104 28-29 0.8654605534238655 1104 30-31 0.8661040240472886 1104 32-33 0.7346975296844249 1104 34-35 0.5965102264174433 1104 36-37 0.5158486441200338 1104 38-39 0.6855812841492082 1104 40-41 0.5788840834356748 1104 42-43 0.6189469077423908 1104 44-45 0.6717038451278725 1104 46-47 0.6294024609386355 1104 48-49 0.5784319625641317 1104 50-51 0.769568999497622 1104 52-53 0.7693333810831788 1104 54-55 0.785935314833317 1104 56-57 0.6837878847640724 1104 58-59 0.6690914169100566 1104 60-61 0.6272892181136953 1104 62-63 0.5297002854234307 1104 64-65 0.533093066736285 1104 66-67 0.6204628402921699 1104 68-69 0.5775298561073612 1104 70-71 0.4748952151126886 1104 72-73 0.5007613497314551 1104 74-75 0.4655798693277404 1104 76-77 0.4685996793602101 1104 78-79 0.4916889653023233 1104 80-81 0.5393742514775965 1104 82-83 0.5199412126460672 1104 84-85 0.5110498535578074 1104 86-87 0.4625568372035751 1104 88-89 0.43377135327184035 1104 90-91 0.4570523566568383 1104 92-93 0.4673281676337808 1104 94-95 0.451113429161623 1104 96-97 0.4692842847340195 1104 98-99 0.5243559122419121 1104 100 0.6852428595924565 1105 1 0.42275404788158966 1105 2 0.5816108161574718 1105 3 0.5160764883984754 1105 4 0.6534304508184192 1105 5 0.5829132705437345 1105 6 0.26395352576316355 1105 7 0.3575956112884171 1105 8 0.3769401106458403 1105 9 0.38961869402721305 1105 10-11 0.4763611334410598 1105 12-13 0.3734586298960565 1105 14-15 0.6243828838652021 1105 16-17 0.6976001259615785 1105 18-19 0.6956946464456948 1105 20-21 0.5391283425343687 1105 22-23 0.7823791895647645 1105 24-25 0.5756488482209576 1105 26-27 0.43480061607986187 1105 28-29 0.758702002598838 1105 30-31 0.7060461651308216 1105 32-33 0.5475689672991422 1105 34-35 0.4819465355354424 1105 36-37 0.4567898353636153 1105 38-39 0.5504032653593072 1105 40-41 0.42772272138566336 1105 42-43 0.4760364197134308 1105 44-45 0.5504328437028221 1105 46-47 0.5523590369768385 1105 48-49 0.5403740198555695 1105 50-51 0.5151349910141363 1105 52-53 0.5735615516815358 1105 54-55 0.46754310166150503 1105 56-57 0.418476496487834 1105 58-59 0.5184542262332457 1105 60-61 0.538538319437528 1105 62-63 0.4439929994766487 1105 64-65 0.4001481603957302 1105 66-67 0.4820865111370267 1105 68-69 0.5361938416621683 1105 70-71 0.48433765517632565 1105 72-73 0.5097571143467334 1105 74-75 0.4950127568565037 1105 76-77 0.459591999084509 1105 78-79 0.39022699909008196 1105 80-81 0.5012172469698868 1105 82-83 0.4716673122732331 1105 84-85 0.41270628923248864 1105 86-87 0.47668151396554137 1105 88-89 0.43160514504917913 1105 90-91 0.42177976577095677 1105 92-93 0.4560067308958189 1105 94-95 0.3920854834367802 1105 96-97 0.46817751111944617 1105 98-99 0.40778253489889593 1105 100 0.5341760578698675 1106 1 0.5475686638124735 1106 2 0.612027630722995 1106 3 0.6201679366824138 1106 4 0.784273272432209 1106 5 0.5750386920408843 1106 6 0.3332829669353572 1106 7 0.4229597295206915 1106 8 0.4618007986748225 1106 9 0.6282208555558668 1106 10-11 0.5895057128283838 1106 12-13 0.4442149012159504 1106 14-15 0.6794936732355268 1106 16-17 0.765706032277599 1106 18-19 0.7491764480602896 1106 20-21 0.5714383499718991 1106 22-23 0.8948874538144622 1106 24-25 0.7376204700768909 1106 26-27 0.6091596568598021 1106 28-29 0.8387518062988448 1106 30-31 0.7549732945768071 1106 32-33 0.7407212852680374 1106 34-35 0.6760565204760169 1106 36-37 0.5155263929036877 1106 38-39 0.6725662619173178 1106 40-41 0.6080161527161323 1106 42-43 0.5934928195973939 1106 44-45 0.6227627063429964 1106 46-47 0.7383153900699284 1106 48-49 0.6848068502044384 1106 50-51 0.7593001915276432 1106 52-53 0.6254332685894681 1106 54-55 0.6829367529907984 1106 56-57 0.6164698911557309 1106 58-59 0.6037152621716757 1106 60-61 0.6151617704668482 1106 62-63 0.5605367146445701 1106 64-65 0.587007309109751 1106 66-67 0.6055075522842941 1106 68-69 0.5972481813938373 1106 70-71 0.6145837180329252 1106 72-73 0.5596399562824743 1106 74-75 0.5592491390182772 1106 76-77 0.5697837646829669 1106 78-79 0.5692057641982231 1106 80-81 0.613497624461445 1106 82-83 0.603689240743627 1106 84-85 0.5977145435639102 1106 86-87 0.6441399791826505 1106 88-89 0.6305765974777877 1106 90-91 0.5963051125427157 1106 92-93 0.6204326569643577 1106 94-95 0.6072325783298567 1106 96-97 0.6048298989829384 1106 98-99 0.6608877107637632 1106 100 0.5660586171933524 1107 1 0.48965493388109493 1107 2 0.6537549762773658 1107 3 0.5606903294364081 1107 4 0.7185121228463842 1107 5 0.584926046023611 1107 6 0.39708081982131205 1107 7 0.37789903358918764 1107 8 0.36818850041044726 1107 9 0.5589234055971417 1107 10-11 0.5396977795674474 1107 12-13 0.4358226647206749 1107 14-15 0.6405999876410036 1107 16-17 0.7848847771494505 1107 18-19 0.903838614054898 1107 20-21 0.6101258601312907 1107 22-23 0.9074180925156625 1107 24-25 0.6363224849033244 1107 26-27 0.5835788607631045 1107 28-29 0.781301201896099 1107 30-31 0.7513987794361512 1107 32-33 0.6323014407954517 1107 34-35 0.5312350956629999 1107 36-37 0.4447128972610912 1107 38-39 0.5748148182582185 1107 40-41 0.4029847229647743 1107 42-43 0.5280384137542384 1107 44-45 0.4236190114437619 1107 46-47 0.46056317468358543 1107 48-49 0.4792892263442994 1107 50-51 0.5219105595226381 1107 52-53 0.5685440392738741 1107 54-55 0.5406688951451031 1107 56-57 0.4114775531349153 1107 58-59 0.418924460304261 1107 60-61 0.42166694954145356 1107 62-63 0.43672788277920915 1107 64-65 0.48266601041765256 1107 66-67 0.46166850414623184 1107 68-69 0.5052046952176852 1107 70-71 0.4329449730683308 1107 72-73 0.35588403173004934 1107 74-75 0.3562828674228413 1107 76-77 0.39453300981124073 1107 78-79 0.3669024170067061 1107 80-81 0.43974995406103545 1107 82-83 0.4646780064640055 1107 84-85 0.40148400009788787 1107 86-87 0.5662601107163674 1107 88-89 0.5522539817195913 1107 90-91 0.5271660658434669 1107 92-93 0.5750405434222365 1107 94-95 0.5330417355335513 1107 96-97 0.5382578858319462 1107 98-99 0.5689201487547635 1107 100 0.533907437610047 1108 1 0.34043566139201786 1108 2 0.46765152943020283 1108 3 0.4231156867105774 1108 4 0.5605829541794733 1108 5 0.4194520665298498 1108 6 0.225220357641156 1108 7 0.17086109653474324 1108 8 0.1431980182971131 1108 9 0.27674365751425256 1108 10-11 0.3401520330447596 1108 12-13 0.18373647970125973 1108 14-15 0.4016140481513517 1108 16-17 0.5718007815255106 1108 18-19 0.6375618275684687 1108 20-21 0.28950085905695744 1108 22-23 0.47183541025042786 1108 24-25 0.3137508292794635 1108 26-27 0.2595840714022515 1108 28-29 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-1.142126426743431 2214 80-81 -1.0445438777748706 2214 82-83 -1.1774134214786898 2214 84-85 -1.195985751910161 2214 86-87 -1.0699697299865782 2214 88-89 -1.0048165408305856 2214 90-91 -0.98901082017424 2214 92-93 -0.9983018673548081 2214 94-95 -1.1618026282779326 2214 96-97 -1.0761278865538024 2214 98-99 -1.269984562250844 2214 100 -1.188507453535454 2215 1 -4.00198419905011 2215 2 -2.163216546913656 2215 3 -3.7981396123902122 2215 4 -1.9718397656508841 2215 5 -1.4578364515167124 2215 6 -1.3598068053440926 2215 7 -1.187327682513704 2215 8 -1.1946067029293772 2215 9 -1.7929659299290748 2215 10-11 -1.4204403042037796 2215 12-13 -1.3457319399376004 2215 14-15 -3.5907861081346013 2215 16-17 -3.3962945447280433 2215 18-19 -2.2838534444998615 2215 20-21 -1.6841451328013122 2215 22-23 -3.183749292539801 2215 24-25 -5.421131403502162 2215 26-27 -3.536752302842949 2215 28-29 -1.760716750031122 2215 30-31 -3.9422785018992386 2215 32-33 -4.0015955323347185 2215 34-35 -2.932013305169818 2215 36-37 -1.9224572656016434 2215 38-39 -1.860510025178101 2215 40-41 -2.022048181843857 2215 42-43 -1.8383503200565556 2215 44-45 -3.3629289836898693 2215 46-47 -2.78023096114768 2215 48-49 -1.9997787856613272 2215 50-51 -5.243798649706996 2215 52-53 -5.508651523486989 2215 54-55 -3.1065612858898675 2215 56-57 -1.9146508270630491 2215 58-59 -1.6693549483859087 2215 60-61 -3.1643452142937853 2215 62-63 -2.760549306012276 2215 64-65 -1.8665209922807406 2215 66-67 -1.682077511402312 2215 68-69 -1.7670016497397114 2215 70-71 -1.604037799571536 2215 72-73 -1.4592611713520895 2215 74-75 -1.5993118472921388 2215 76-77 -1.6539542134612901 2215 78-79 -1.5221440802805333 2215 80-81 -1.536871347838666 2215 82-83 -1.5321698255690634 2215 84-85 -1.420746409130075 2215 86-87 -1.4364031964527015 2215 88-89 -1.2601420701628676 2215 90-91 -1.5538551446796873 2215 92-93 -3.893155904230696 2215 94-95 -1.9348326650562413 2215 96-97 -1.634052878376142 2215 98-99 -3.1591571175788715 2215 100 -3.1743381966543964 2216 1 -0.8885342119074942 2216 2 -0.8582215612071273 2216 3 -1.0948019890213345 2216 4 -1.045523164497034 2216 5 -1.1778293760516974 2216 6 -1.0247223869393949 2216 7 -1.156296425392192 2216 8 -1.03845594325076 2216 9 -1.2883933036865827 2216 10-11 -1.2941143267288098 2216 12-13 -1.4787152644266044 2216 14-15 -1.8797144365536766 2216 16-17 -1.61962944974443 2216 18-19 -3.6972102235511173 2216 20-21 -1.8727322822510786 2216 22-23 -1.363203405032408 2216 24-25 -1.4721634985076903 2216 26-27 -1.472031198233104 2216 28-29 -1.5078805502266022 2216 30-31 -1.5963570599152987 2216 32-33 -1.6133873302424107 2216 34-35 -1.7960280689290329 2216 36-37 -1.762415954276733 2216 38-39 -1.7647999479661394 2216 40-41 -1.830910690396891 2216 42-43 -1.7607172579744415 2216 44-45 -1.7030908982704531 2216 46-47 -1.876405257329445 2216 48-49 -1.7455458811985451 2216 50-51 -1.714275627181216 2216 52-53 -1.7377965093987982 2216 54-55 -1.651500029860678 2216 56-57 -1.6415345380715962 2216 58-59 -1.6923529708292833 2216 60-61 -1.7050911698843123 2216 62-63 -1.7339349235394508 2216 64-65 -1.7326405131683629 2216 66-67 -1.781097866548322 2216 68-69 -1.8098153111003015 2216 70-71 -1.8304386141405153 2216 72-73 -1.822883462490129 2216 74-75 -1.8862013824330468 2216 76-77 -1.7499310806921784 2216 78-79 -1.7701755154451675 2216 80-81 -1.7529916464723243 2216 82-83 -1.9409371513075513 2216 84-85 -1.8283044843514737 2216 86-87 -1.8169178711903449 2216 88-89 -1.7335258372905535 2216 90-91 -1.7354275887156518 2216 92-93 -1.6616850962774699 2216 94-95 -1.6267997570892696 2216 96-97 -1.66457995946406 2216 98-99 -1.5437082797656316 2216 100 -1.5579740327166327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15412.0 1 2663.0 2 437.0 3 393.0 4 405.0 5 198.0 6 182.0 7 280.0 8 435.0 9 790.0 10 1451.0 11 2681.0 12 4536.0 13 6275.0 14 8387.0 15 10563.0 16 12811.0 17 14987.0 18 17619.0 19 19701.0 20 22522.0 21 25511.0 22 29563.0 23 33720.0 24 38292.0 25 42467.0 26 48250.0 27 55172.0 28 65086.0 29 77407.0 30 92690.0 31 111115.0 32 136546.0 33 174924.0 34 236755.0 35 321626.0 36 472883.0 37 728943.0 38 671662.0 39 217546.0 40 297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2153906925714 20.023326630341007 12.69323554850409 24.068047128583505 2 15.491453683258543 19.680352213149607 40.63561004880218 24.19258405478967 3 18.82459850673438 25.78773756007825 28.970967038471667 26.416696894715706 4 12.301704024450993 16.5520773684202 39.22213844733165 31.924080159797157 5 13.420586433416231 38.05760959848845 34.99784322119972 13.5239607468956 6 30.81185866490826 37.67185830322205 18.03337715854461 13.482905873325077 7 27.52422986931893 31.609609500975782 22.96123139920021 17.904929230505083 8 26.03915254438624 35.30818482126177 20.00326127217692 18.649401362175073 9 25.9829138293219 15.565641892439208 21.039318713245024 37.41212556499387 10-11 25.381663645043258 25.937559537368234 27.834653850500597 20.84612296708791 12-13 25.727152779990615 23.636509212987242 27.686380916529803 22.949957090492344 14-15 23.05529437243231 25.011206421198334 25.08107778228435 26.852421424085005 16-17 22.48508206232851 27.742928486391538 26.219815590485414 23.55217386079454 18-19 22.09030114784972 27.972254582514424 27.652022711794288 22.28542155784157 20-21 23.47930804156417 27.115409983658466 26.884781475404058 22.520500499373302 22-23 23.64155403982763 26.899116873132712 26.63644088751065 22.82288819952901 24-25 23.1857874418886 26.98760164094499 27.19644623939305 22.63016467777336 26-27 23.085404565990117 27.18693658791024 26.44018861801612 23.287470228083524 28-29 22.49451535531763 27.59506278704864 26.85536643339405 23.05505542423968 30-31 23.063124775051953 27.275475188821353 26.77385961298001 22.887540423146678 32-33 23.302876301446446 27.445890379796428 26.100873938021458 23.15035938073567 34-35 23.402353225835633 27.318747844703623 26.747710761318867 22.531188168141878 36-37 23.155534968392253 26.986291380085703 26.545781604709358 23.31239204681269 38-39 23.196678410913236 27.073994694834134 26.941068476177254 22.788258418075376 40-41 23.030651431022196 26.448286935270115 27.416098219834375 23.104963413873318 42-43 22.89773589654813 26.658309805749568 26.95113302397734 23.49282127372496 44-45 23.057972527437652 27.28770670832311 26.807997680391242 22.846323083847995 46-47 23.076948008715405 27.115548282213016 26.464485690262745 23.343018018808834 48-49 23.491794202079493 26.844304706815826 26.555897834140264 23.10800325696442 50-51 23.21689131655339 26.795774524268047 26.638329210858704 23.34900494831986 52-53 22.922118857041323 27.17685599277492 26.46308402148566 23.43794112869809 54-55 22.80718621479543 27.08937289882535 26.56778669450836 23.53565419187086 56-57 23.526068930729704 26.76069801582035 26.36661370894387 23.346619344506074 58-59 22.85485517382921 26.951764216620848 26.928769720177364 23.264610889372573 60-61 22.818480236036947 27.340276025408023 26.60400394943076 23.237239789124267 62-63 23.10137993705209 26.90583613482079 26.319288971884593 23.673494956242525 64-65 22.55818066927348 27.05856142928125 26.57214727040982 23.81111063103545 66-67 22.995186849816967 26.800003132436164 26.52447026724001 23.68033975050686 68-69 22.738620412667792 26.80273077144347 26.72484879390298 23.733800021985758 70-71 22.627666263975645 26.54791534819901 26.846794272056652 23.977624115768695 72-73 23.01329327768969 26.656325980346484 27.066888225795864 23.263492516167954 74-75 23.135363731925814 26.908256688723302 26.74889801220238 23.20748156714851 76-77 22.930649449153805 26.804387328154633 26.553282847818316 23.711680374873243 78-79 22.810503328376893 26.815697460061504 26.632673870384398 23.741125341177202 80-81 22.568845441832877 27.023406349824864 27.07402467241547 23.33372353592679 82-83 23.1999788291179 26.36251436089252 27.679429639153923 22.75807717083565 84-85 23.299189268053844 26.73287353913033 26.78752006710437 23.180417125711454 86-87 22.42531038980476 27.13806141224737 27.163945006504758 23.272683191443107 88-89 23.13588956172035 26.254732289192102 26.959978509472183 23.649399639615364 90-91 22.86279044881903 26.342880205020457 27.127309970141017 23.667019376019493 92-93 23.005308855797225 26.507423625616894 27.136259726814053 23.35100779177183 94-95 22.24502367467067 26.471073933471907 27.57536226333817 23.708540128519253 96-97 22.60492294029341 26.281708750362398 27.27963178095883 23.833736528385362 98-99 22.10153640632704 26.79346417281098 27.200979089518988 23.90402033134299 100 22.755264896624748 26.3022826930874 26.67443523326474 24.26801717702311 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13012.0 1 7733.0 2 2276.5 3 1739.0 4 1020.0 5 585.5 6 474.0 7 438.0 8 456.0 9 474.5 10 438.0 11 410.0 12 418.0 13 421.5 14 446.0 15 537.5 16 625.0 17 721.5 18 939.5 19 1199.5 20 1531.0 21 2092.0 22 2945.5 23 4083.5 24 5470.0 25 7171.5 26 9728.5 27 13001.5 28 17310.5 29 24653.0 30 34173.5 31 43288.5 32 54536.0 33 64421.0 34 71486.0 35 79944.0 36 88661.5 37 102961.5 38 110888.0 39 108132.5 40 105145.0 41 102043.5 42 102528.0 43 108312.5 44 117063.0 45 133133.5 46 148919.5 47 158947.0 48 165668.0 49 163894.5 50 157995.5 51 158800.0 52 158872.5 53 153495.0 54 146495.5 55 130647.0 56 116144.0 57 99849.0 58 83283.5 59 76318.5 60 64571.0 61 49506.5 62 39165.0 63 31744.0 64 23385.5 65 14478.5 66 9698.5 67 7137.5 68 5381.5 69 4057.5 70 3028.5 71 2303.0 72 1720.0 73 1331.0 74 1068.5 75 835.5 76 618.5 77 396.0 78 257.0 79 169.5 80 105.0 81 62.5 82 46.5 83 41.0 84 34.5 85 27.0 86 21.5 87 13.0 88 11.5 89 9.0 90 9.5 91 9.5 92 7.5 93 5.5 94 5.5 95 4.5 96 3.5 97 4.0 98 2.5 99 2.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0009446218464149 2 0.4509850845365377 3 0.4838601809258368 4 0.4696250493193593 5 0.43688424662446085 6 0.49189094385100063 7 0.5096177115118972 8 0.5131899237829567 9 0.5265118582675093 10-11 0.5412438765432696 12-13 0.5382759858970134 14-15 0.53580498192004 16-17 0.5418616275375129 18-19 0.5394577704077398 20-21 0.5382894152664535 22-23 0.5000828592094452 24-25 0.5358586993978003 26-27 0.5153923403711287 28-29 0.5219593020273244 30-31 0.5250883451068615 32-33 0.5280293770142376 34-35 0.5397532165354215 36-37 0.5578022890628799 38-39 0.5473005221607425 40-41 0.5519470839870079 42-43 0.5489120464935514 44-45 0.5595883951984095 46-47 0.5579634414961607 48-49 0.5300437824302485 50-51 0.5455009866557727 52-53 0.536355586067083 54-55 0.5464007544082576 56-57 0.5348783554286749 58-59 0.5404784024851854 60-61 0.5335354184846676 62-63 0.5276264959310354 64-65 0.5388668781523765 66-67 0.5370404839085267 68-69 0.5582857463627224 70-71 0.5419690624930336 72-73 0.5461455963888963 74-75 0.542962835831599 76-77 0.5303123698190501 78-79 0.5620996872837032 80-81 0.5365973147170042 82-83 0.536731608411405 84-85 0.5450846762031305 86-87 0.8502268086204735 88-89 1.1162760465977632 90-91 1.1068217705119516 92-93 1.1071709341173936 94-95 1.1119517896380597 96-97 1.104847653204261 98-99 1.100845701111119 100 1.096427438565335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3723183.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.997626738566176 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6196535126581 33.71082916088202 2 12.776063517798281 11.242329476886031 3 4.501060062706408 5.941078817004715 4 2.0671210480277336 3.6379368117823185 5 1.1184615699398044 2.4604827337821122 6 0.6754872922078907 1.7831902649524536 7 0.42160308069208174 1.2984674483283867 8 0.28817966188080396 1.0143376957662256 9 0.1993103276148597 0.7892263259585943 >10 1.0615577753594372 8.687028026919737 >50 0.112840051403517 3.4966278221235365 >100 0.12896378676181808 12.207830529323148 >500 0.02071548989861328 6.098665683985237 >1k 0.008555069572255974 6.265734735272306 >5k 3.666458388109703E-4 1.0573691183776344 >10k+ 6.110763980182839E-5 0.3088653486554684 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11488 0.3085531922551215 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 8500 0.22829928048124412 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7153 0.192120559209687 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6737 0.18094732383554607 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6000 0.16115243328087822 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5727 0.15381999756659825 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5211 0.13996088830444273 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4659 0.12513486444260194 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 4113 0.11046999301404202 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.371747776029274E-5 2 0.0 0.0 0.0 0.0 5.371747776029274E-5 3 0.0 0.0 0.0 0.0 5.371747776029274E-5 4 0.0 0.0 0.0 0.0 5.371747776029274E-5 5 0.0 0.0 0.0 0.0 5.371747776029274E-5 6 0.0 0.0 0.0 0.0 5.371747776029274E-5 7 0.0 0.0 0.0 0.0 5.371747776029274E-5 8 0.0 0.0 0.0 0.0 5.371747776029274E-5 9 0.0 0.0 0.0 0.0 5.371747776029274E-5 10-11 0.0 0.0 0.0 0.0 8.05762166404391E-5 12-13 2.685873888014637E-5 0.0 0.0 0.0 2.2829928048124414E-4 14-15 2.685873888014637E-5 0.0 0.0 0.0 2.9544612768161007E-4 16-17 2.685873888014637E-5 0.0 0.0 0.0 3.491636054419028E-4 18-19 2.685873888014637E-5 0.0 0.0 1.3429369440073185E-5 3.8945171376212235E-4 20-21 2.685873888014637E-5 0.0 0.0 2.685873888014637E-5 4.834572998426346E-4 22-23 2.685873888014637E-5 0.0 0.0 6.714684720036593E-5 5.640335164830738E-4 24-25 2.685873888014637E-5 0.0 0.0 2.685873888014637E-4 5.640335164830738E-4 26-27 2.685873888014637E-5 0.0 0.0 4.297398220823419E-4 6.043216248032933E-4 28-29 2.685873888014637E-5 0.0 0.0 0.001396654421767611 6.177509942433665E-4 30-31 2.685873888014637E-5 0.0 0.0 0.006002928139712713 6.177509942433665E-4 32-33 2.685873888014637E-5 0.0 0.0 0.016007808372567236 6.177509942433665E-4 34-35 2.685873888014637E-5 0.0 0.0 0.032311062872816085 7.25185949763952E-4 36-37 2.685873888014637E-5 0.0 0.0 0.06122449527729365 7.520446886440983E-4 38-39 2.685873888014637E-5 0.0 0.0 0.10801241840650863 8.057621664043911E-4 40-41 2.685873888014637E-5 0.0 0.0 0.16175675490568153 8.326209052845375E-4 42-43 2.685873888014637E-5 0.0 0.0 0.21618598924629812 8.326209052845375E-4 44-45 2.685873888014637E-5 0.0 0.0 0.2837491468993063 8.460502747246107E-4 46-47 2.685873888014637E-5 0.0 0.0 0.3710131895209019 8.863383830448302E-4 48-49 2.685873888014637E-5 0.0 0.0 0.4735598545653007 9.131971219249765E-4 50-51 2.685873888014637E-5 0.0 0.0 0.5832240854129384 9.131971219249765E-4 52-53 2.685873888014637E-5 0.0 0.0 0.6963530935761149 9.803439691253423E-4 54-55 2.685873888014637E-5 0.0 0.0 0.8208567776550333 9.937733385654156E-4 56-57 2.685873888014637E-5 0.0 0.0 0.9749319332409929 0.0010072027080054889 58-59 2.685873888014637E-5 0.0 0.0 1.1603780958389636 0.001020632077445562 60-61 2.685873888014637E-5 0.0 0.0 1.3545802073118618 0.001020632077445562 62-63 2.685873888014637E-5 0.0 0.0 1.5581694480233712 0.0010743495552058549 64-65 2.685873888014637E-5 0.0 0.0 1.7734153814088645 0.0010743495552058549 66-67 2.685873888014637E-5 0.0 0.0 2.0237656865107088 0.0010877789246459281 68-69 2.685873888014637E-5 0.0 0.0 2.3087234766596216 0.0011012082940860012 70-71 2.685873888014637E-5 0.0 0.0 2.6255625898592685 0.0011280670329661476 72-73 2.685873888014637E-5 0.0 0.0 2.951627679864245 0.001168355141286367 74-75 2.685873888014637E-5 0.0 0.0 3.2766184203140165 0.0012220726190466597 76-77 2.685873888014637E-5 0.0 0.0 3.646946711993474 0.0012623607273668793 78-79 2.685873888014637E-5 0.0 0.0 4.057495965145952 0.0012757900968069524 80-81 2.685873888014637E-5 0.0 0.0 4.50870934896297 0.001302648835687099 82-83 2.685873888014637E-5 0.0 0.0 4.979945922615139 0.0013429369440073184 84-85 2.685873888014637E-5 0.0 0.0 5.438787188274119 0.0013429369440073184 86-87 2.685873888014637E-5 0.0 0.0 5.92706563174574 0.0013429369440073184 88 2.685873888014637E-5 0.0 0.0 6.319807541020681 0.0013429369440073184 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6720 0.0 32.78059 1 CAACGCA 6215 0.0 31.973272 5 GGTATCA 4180 0.0 31.258823 1 GTACTAG 2775 0.0 31.106937 1 ATCAACG 6695 0.0 29.896694 3 AACGCAG 7675 0.0 27.280052 6 GGGCACC 3195 0.0 27.192694 94 TATCAAC 7950 0.0 27.12896 2 TAGGCAT 3520 0.0 26.825094 5 GCATCGT 2965 0.0 26.600927 8 CTGTGCG 3680 0.0 26.155628 9 TAACGCC 1475 0.0 26.117947 4 CTAACGC 1500 0.0 25.685423 3 GTACATG 13250 0.0 25.632193 1 ACGCAGA 7660 0.0 25.618856 7 TAGTACT 3695 0.0 25.556349 4 TACATGG 13470 0.0 24.906816 2 TCTAACG 1555 0.0 24.778275 2 ACATGGG 13285 0.0 24.509577 3 CGCAGAG 7980 0.0 24.356207 8 >>END_MODULE