FastQCFastQC Report
Mon 16 Jan 2017
ERR1146467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723183
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC97220.261120659392783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79600.2137955614859651No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA75070.2016285527732588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74700.20063477943469335No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT69060.18548645070629083No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA62550.16800141169531554No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC62460.16775968304539424No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60870.16348914356345096No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC50180.13477715170057447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.11812473359488374No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG41770.11218895230237139No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38410.1031644160386422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69350.047.931081
GTATCAA118900.038.593331
CAACGCA141700.031.5098515
GTGGTAT21550.031.4166221
ATCAACG143700.031.0452633
GGGCACC32250.030.19302694
TATCAAC153400.029.7886032
GTACTAG26750.029.527711
AACGCAG158850.028.7876726
TAGTACT36900.028.0273634
CTGTGCG37150.027.7088649
TCAACGC164000.027.5177734
CTAACGC13050.026.6567463
CTAGTAC43350.025.9175953
ACGCAGA172100.025.9158977
CGCAGAG173550.025.6448678
GTACTGT45000.025.379016
TCTAACG14150.025.2502922
ACTGTGC49600.025.1095718
TAACGCC14700.024.3042474
Produced by FastQC (version 0.11.5)