FastQCFastQC Report
Mon 16 Jan 2017
ERR1146466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5717861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC205830.3599772712208289No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175470.3068804925478251No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT164070.28694296695914784No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA151600.2651341122143403No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC133500.23347891807793159No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA123120.2153252763577149No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC119800.20951890925645097No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC100900.17646459051732807No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC93770.1639948924956378No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG93270.16312043961894143No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT89210.15601988226016686No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT78500.13728910164133057No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT77870.13618729101669313No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG73970.1293665585784614No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC73960.12934906952092748No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC71910.12576381272647236No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT68200.11927537238138528No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68020.11896056934577459No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT67830.11862827725262995No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG66190.11576007181706585No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG64820.1133640709349178No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA63480.11102053722537153No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA61430.1074352804309164No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA59660.10433971724741122No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA59540.10412984855700409No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC59010.10320292850770595No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT59010.10320292850770595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59900.039.33202494
GTACTAG59600.033.0863881
GTATCAA118950.031.966121
CAACGCA105550.031.7367155
TAGGCAT73800.030.7483235
ATCAACG113550.029.5881883
TAGTACT86900.029.1960284
GCATCGT64400.029.0139528
CTGTGCG88550.028.8966989
AGGGCTA63700.028.248155
CTATTGA62950.028.043519
TATTCTC29750.027.4784975
CTAGGCA84600.027.3240174
TATCAAC139350.027.0446992
GCTATTG64550.026.9828228
GGTATCA79500.026.5846061
ACTGTGC98350.026.4928288
CTAGTAC103050.025.99123
ACGCAGA128900.025.8221027
GTACTGT100100.025.7933526
Produced by FastQC (version 0.11.5)