##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146466_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5717861
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.31983638636896	31.0	30.0	34.0	26.0	34.0
2	30.440361177020566	31.0	30.0	34.0	26.0	34.0
3	30.644677441441825	31.0	30.0	34.0	26.0	34.0
4	34.11749253785638	37.0	35.0	37.0	29.0	37.0
5	34.32238226847417	37.0	35.0	37.0	30.0	37.0
6	34.459384724462524	37.0	35.0	37.0	30.0	37.0
7	34.31836800509841	37.0	35.0	37.0	30.0	37.0
8	34.27238874816999	37.0	35.0	37.0	30.0	37.0
9	35.63733011348125	39.0	35.0	39.0	29.0	39.0
10-11	35.55539169280261	39.0	35.0	39.0	29.5	39.0
12-13	35.53524972013136	39.0	35.0	39.0	29.0	39.0
14-15	36.5153347554269	40.0	36.0	41.0	28.0	41.0
16-17	36.34485623207699	39.5	36.0	41.0	27.5	41.0
18-19	36.292018641236645	39.0	35.0	41.0	27.0	41.0
20-21	36.626943711993	40.0	36.0	41.0	29.0	41.0
22-23	36.329993156531785	39.0	35.5	41.0	27.5	41.0
24-25	36.43403118753673	39.0	35.0	41.0	28.5	41.0
26-27	36.498926958875	39.0	35.0	41.0	28.5	41.0
28-29	36.325204652579	39.0	35.0	41.0	28.0	41.0
30-31	36.26470117059509	39.0	35.0	41.0	28.0	41.0
32-33	36.36009864528012	39.0	35.0	41.0	28.5	41.0
34-35	36.40973294034255	39.0	35.0	41.0	29.0	41.0
36-37	36.343304078220854	39.0	35.0	41.0	29.0	41.0
38-39	36.172786554272655	39.0	35.0	41.0	28.0	41.0
40-41	36.134616773650144	39.0	35.0	41.0	28.0	41.0
42-43	35.9746515349009	39.0	35.0	41.0	27.5	41.0
44-45	35.79130097776074	38.5	34.0	40.5	27.0	41.0
46-47	35.66288285077235	38.0	34.0	40.0	27.0	41.0
48-49	35.531143988984695	38.0	34.0	40.0	27.0	41.0
50-51	35.30621923128247	38.0	33.5	40.0	26.5	41.0
52-53	35.11759992066963	38.0	33.0	40.0	26.0	41.0
54-55	34.915403942138504	37.0	33.0	40.0	26.0	41.0
56-57	34.76690461695378	37.0	33.0	40.0	26.0	41.0
58-59	34.500292504487255	36.5	33.0	40.0	26.0	41.0
60-61	34.24929951952312	36.0	33.0	39.5	26.0	41.0
62-63	33.97725119585803	35.5	32.0	39.0	26.0	41.0
64-65	33.68633497736304	35.0	32.0	39.0	25.0	41.0
66-67	33.39787667101386	35.0	32.0	38.5	25.0	40.0
68-69	33.11365089147847	35.0	31.5	38.0	24.0	40.0
70-71	33.060610952242456	35.0	32.0	37.0	25.0	40.0
72-73	32.85745596124145	35.0	31.0	37.0	25.0	40.0
74-75	32.59952454248188	35.0	31.0	36.0	24.0	39.0
76-77	32.37591408745334	35.0	31.0	36.0	24.0	39.0
78-79	32.161697355007405	35.0	31.0	36.0	24.0	39.0
80-81	31.950682956441227	35.0	31.0	35.0	23.5	37.5
82-83	31.72226782008167	34.5	31.0	35.0	23.0	37.0
84-85	31.551759827669823	34.0	31.0	35.0	23.0	36.5
86-87	31.3902328160828	34.0	31.0	35.0	23.0	36.0
88-89	31.40422878415547	35.0	31.0	35.0	23.0	36.0
90-91	31.334021935825305	35.0	31.0	35.0	23.0	36.0
92-93	31.194980080837922	34.0	31.0	35.0	23.0	35.5
94-95	31.1848388759363	34.0	31.0	35.0	23.0	35.0
96-97	31.099777784034973	34.0	31.0	35.0	22.5	35.0
98-99	31.021023158835096	34.0	31.0	35.0	21.5	35.0
100	30.517900487612412	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7309710220196806
1101	2	0.897777496749228
1101	3	0.8623906873766565
1101	4	0.9139504304125268
1101	5	0.8633050456771656
1101	6	0.6229294302882451
1101	7	0.5708242384516566
1101	8	0.6678868431839717
1101	9	0.8869223280682448
1101	10-11	0.8831617947965071
1101	12-13	0.7593475411712163
1101	14-15	0.9352551853304902
1101	16-17	1.0650741032330728
1101	18-19	1.2379909544891916
1101	20-21	0.9065234413647119
1101	22-23	1.2054134376228518
1101	24-25	1.062793833061619
1101	26-27	0.9669789445824151
1101	28-29	1.171574263499771
1101	30-31	1.1817701242374952
1101	32-33	0.9991223274846988
1101	34-35	0.8872567352796779
1101	36-37	0.8981884247065253
1101	38-39	0.9870866952607358
1101	40-41	0.835982175556417
1101	42-43	0.9060034985191621
1101	44-45	0.899653889610839
1101	46-47	0.8818074780750038
1101	48-49	0.9497852325883187
1101	50-51	0.9426460797311975
1101	52-53	0.9741080972758951
1101	54-55	0.9795256442544868
1101	56-57	0.8346254725306395
1101	58-59	0.8026007079147064
1101	60-61	0.8014538373379381
1101	62-63	0.7982287929942018
1101	64-65	0.7387075956399514
1101	66-67	0.8296319233162208
1101	68-69	0.7662219511616115
1101	70-71	0.7001822748623283
1101	72-73	0.693874310224615
1101	74-75	0.7282485758365596
1101	76-77	0.720940471656256
1101	78-79	0.675794916584934
1101	80-81	0.7489469697586735
1101	82-83	0.7353567740348339
1101	84-85	0.7298436899591856
1101	86-87	0.7403661091305658
1101	88-89	0.7257831000689272
1101	90-91	0.7949362313896735
1101	92-93	0.8997770490077137
1101	94-95	0.826024070731215
1101	96-97	0.7737576639351609
1101	98-99	0.822856725270583
1101	100	0.8939979679671453
1102	1	0.6916277964045889
1102	2	0.8138319603244106
1102	3	0.8225155401015378
1102	4	0.8920592169311021
1102	5	0.7859663677809081
1102	6	0.5492319216690831
1102	7	0.5903562275140075
1102	8	0.6541482152450371
1102	9	0.7511039136232398
1102	10-11	0.7465500503148377
1102	12-13	0.7151476217158219
1102	14-15	0.8232466873828344
1102	16-17	1.0780911478006203
1102	18-19	1.0957086025377691
1102	20-21	0.8429445147910712
1102	22-23	1.1327815933051042
1102	24-25	1.0178119647030144
1102	26-27	0.8313354418998173
1102	28-29	0.9902916352534561
1102	30-31	1.0795196605310977
1102	32-33	0.9441606098456035
1102	34-35	0.8719885350328127
1102	36-37	0.8199957390735406
1102	38-39	0.8789045213726823
1102	40-41	0.7984872134964363
1102	42-43	0.8445631714667527
1102	44-45	0.8664968823354187
1102	46-47	0.8071052755122139
1102	48-49	0.7488781549514769
1102	50-51	0.8549048377475117
1102	52-53	0.8160786139749305
1102	54-55	0.8772797590154866
1102	56-57	0.7436468573812292
1102	58-59	0.7250064878031708
1102	60-61	0.8025867413757837
1102	62-63	0.7817018914155653
1102	64-65	0.7314274032423498
1102	66-67	0.7377947804942266
1102	68-69	0.8000801273067566
1102	70-71	0.7393274622518504
1102	72-73	0.6600099362393763
1102	74-75	0.6885036222246228
1102	76-77	0.6994119976602136
1102	78-79	0.7290319502677036
1102	80-81	0.7245417614502507
1102	82-83	0.7194686987286119
1102	84-85	0.6657028995892809
1102	86-87	0.7112663653712552
1102	88-89	0.7220995799683756
1102	90-91	0.7050844980326367
1102	92-93	0.768979450850896
1102	94-95	0.7407695933998149
1102	96-97	0.7627746165171452
1102	98-99	0.7878577108117035
1102	100	0.7805741903054297
1103	1	0.6815204687865091
1103	2	0.8223129792624526
1103	3	0.7208769799100061
1103	4	0.7322654779798086
1103	5	0.6809629802457948
1103	6	0.5849438400134659
1103	7	0.6868360727874716
1103	8	0.6539589708214422
1103	9	0.7587898740841581
1103	10-11	0.9155325336237894
1103	12-13	0.7853250977053889
1103	14-15	0.9568765118975193
1103	16-17	1.1829003923920212
1103	18-19	1.2188650262512155
1103	20-21	0.8472701295332001
1103	22-23	1.1098348731535452
1103	24-25	1.0223700376752234
1103	26-27	0.9339928226225425
1103	28-29	1.1506031126059497
1103	30-31	1.1327414107004259
1103	32-33	0.9326891485977669
1103	34-35	0.9368554745505193
1103	36-37	0.9320830185600073
1103	38-39	0.9837652785932178
1103	40-41	0.8495698541885801
1103	42-43	0.9195018657057616
1103	44-45	0.9208525291748373
1103	46-47	0.8746132729977063
1103	48-49	0.8883025900413699
1103	50-51	0.9177071096602987
1103	52-53	0.9199594522096959
1103	54-55	0.9078923309423104
1103	56-57	0.7433548547729245
1103	58-59	0.7254248422662357
1103	60-61	0.7039575922315322
1103	62-63	0.7061434366714607
1103	64-65	0.7536134587814587
1103	66-67	0.7876181432459646
1103	68-69	0.7238204090206466
1103	70-71	0.675778087852791
1103	72-73	0.6628664248686391
1103	74-75	0.6982045550644074
1103	76-77	0.6547002536823143
1103	78-79	0.6575614408440202
1103	80-81	0.7061904589728307
1103	82-83	0.6540979216633431
1103	84-85	0.6157736845078254
1103	86-87	0.5824099076964764
1103	88-89	0.611756505858299
1103	90-91	0.6609781186429835
1103	92-93	0.7015680493160268
1103	94-95	0.6583197090387607
1103	96-97	0.6769958750626515
1103	98-99	0.7464865425943863
1103	100	0.7392091322102843
1104	1	0.5893926968702345
1104	2	0.7160596206468277
1104	3	0.7104358103597761
1104	4	0.7808398927036109
1104	5	0.7448544301854199
1104	6	0.41334449706616283
1104	7	0.5259936346729006
1104	8	0.5084604814763551
1104	9	0.6020617261665748
1104	10-11	0.6678723578136925
1104	12-13	0.6138401156666831
1104	14-15	0.7870966603036322
1104	16-17	0.8992576569118143
1104	18-19	0.8937852922141474
1104	20-21	0.7770958090452638
1104	22-23	1.0378574499432105
1104	24-25	0.875496886819839
1104	26-27	0.7761143775203152
1104	28-29	0.9632946035940151
1104	30-31	0.9804899685876691
1104	32-33	0.7574869349573561
1104	34-35	0.7106013054290656
1104	36-37	0.7019861121632971
1104	38-39	0.8567660453629671
1104	40-41	0.7603875234039137
1104	42-43	0.7584794377774315
1104	44-45	0.7828176108286513
1104	46-47	0.7468430488939646
1104	48-49	0.7103655143017491
1104	50-51	0.8217187118193152
1104	52-53	0.7804117033009774
1104	54-55	0.7600197959126689
1104	56-57	0.6850021112838647
1104	58-59	0.6844261652080945
1104	60-61	0.7181219314729219
1104	62-63	0.6327413306753442
1104	64-65	0.7280650024590898
1104	66-67	0.8140141471671285
1104	68-69	0.6900993502645036
1104	70-71	0.6061597163412245
1104	72-73	0.6783492926995578
1104	74-75	0.6274745831244104
1104	76-77	0.662095611033557
1104	78-79	0.6171514094447872
1104	80-81	0.6287959919630985
1104	82-83	0.5776991569644636
1104	84-85	0.6292070003087069
1104	86-87	0.5243753602639352
1104	88-89	0.4888692694084362
1104	90-91	0.5605631664395183
1104	92-93	0.554056684011158
1104	94-95	0.4672064528875346
1104	96-97	0.5311174471672473
1104	98-99	0.6625396975942905
1104	100	0.6921007437552014
1105	1	0.3985798795557862
1105	2	0.5662914180017964
1105	3	0.57618411577959
1105	4	0.6964713272717873
1105	5	0.6166475228013368
1105	6	0.30366454531764475
1105	7	0.4346240034704394
1105	8	0.45544718888893243
1105	9	0.4641267847304036
1105	10-11	0.5686192614161882
1105	12-13	0.4974554484749092
1105	14-15	0.6093393233707118
1105	16-17	0.777975550594121
1105	18-19	0.7326987429114666
1105	20-21	0.5890938743121978
1105	22-23	0.7824394031898834
1105	24-25	0.6696816240081489
1105	26-27	0.5084419827446993
1105	28-29	0.7035913896501569
1105	30-31	0.7308414818109767
1105	32-33	0.6400505487699846
1105	34-35	0.4919755318659895
1105	36-37	0.4603052300486752
1105	38-39	0.5831130846986241
1105	40-41	0.47275982247133896
1105	42-43	0.5439562269749629
1105	44-45	0.5213967553287873
1105	46-47	0.39345044103238536
1105	48-49	0.5321888858065833
1105	50-51	0.6388004233156366
1105	52-53	0.556712026549647
1105	54-55	0.5401156696282499
1105	56-57	0.4600338917938984
1105	58-59	0.4558284955414962
1105	60-61	0.5354450890385891
1105	62-63	0.4804534351898795
1105	64-65	0.43645814250614734
1105	66-67	0.5278682708986437
1105	68-69	0.471593055231466
1105	70-71	0.462248140312127
1105	72-73	0.37288629468243073
1105	74-75	0.43760119336582903
1105	76-77	0.39868671872239503
1105	78-79	0.4709407373885597
1105	80-81	0.48593104737032533
1105	82-83	0.4201894113369491
1105	84-85	0.38737689935047825
1105	86-87	0.469593057594448
1105	88-89	0.5228066063790884
1105	90-91	0.5369451218891754
1105	92-93	0.6360509902046978
1105	94-95	0.51736031808462
1105	96-97	0.5053781764250544
1105	98-99	0.5763429883322964
1105	100	0.6560053870719571
1106	1	0.5606510337836603
1106	2	0.6270103012662851
1106	3	0.6028260324058152
1106	4	0.740036933118212
1106	5	0.5738473870509608
1106	6	0.3539628011668512
1106	7	0.403286862283764
1106	8	0.48594104754300815
1106	9	0.6030807521206256
1106	10-11	0.5677954823079077
1106	12-13	0.49091153702762114
1106	14-15	0.6826224371159952
1106	16-17	0.7821809316524124
1106	18-19	0.7739275416640581
1106	20-21	0.6166144387580275
1106	22-23	0.9203367768125901
1106	24-25	0.6874415327665844
1106	26-27	0.5845081112401331
1106	28-29	0.8220360503660515
1106	30-31	0.8020253138810247
1106	32-33	0.6424218091880576
1106	34-35	0.6154699002129078
1106	36-37	0.5952067556806213
1106	38-39	0.6828836537357148
1106	40-41	0.6780997103640729
1106	42-43	0.6055334433023347
1106	44-45	0.6278883138240872
1106	46-47	0.6318201799192025
1106	48-49	0.6258090396050164
1106	50-51	0.6421287603509143
1106	52-53	0.6456287103863687
1106	54-55	0.6761645827824765
1106	56-57	0.5718725395430582
1106	58-59	0.5574771544027683
1106	60-61	0.5506801376921118
1106	62-63	0.5039640427633714
1106	64-65	0.5678025039221453
1106	66-67	0.6017949913572735
1106	68-69	0.5411468324805924
1106	70-71	0.5754566756294821
1106	72-73	0.5053929872312253
1106	74-75	0.5194436625534422
1106	76-77	0.5526264921983923
1106	78-79	0.5320990043121299
1106	80-81	0.5505736346979937
1106	82-83	0.5576757664872005
1106	84-85	0.5050307562737082
1106	86-87	0.5886128146073126
1106	88-89	0.6286378094931955
1106	90-91	0.493882070419005
1106	92-93	0.5971005951927424
1106	94-95	0.574609139011109
1106	96-97	0.5728629680308686
1106	98-99	0.5854339174349263
1106	100	0.521008683974582
1107	1	0.5136467481730094
1107	2	0.6381690653197829
1107	3	0.5632799229271122
1107	4	0.6782229361002479
1107	5	0.5975076136612785
1107	6	0.3721184069988155
1107	7	0.39062292261905895
1107	8	0.41476004060999827
1107	9	0.5383384122009431
1107	10-11	0.5562607153609918
1107	12-13	0.4170382802864552
1107	14-15	0.6344453273805968
1107	16-17	0.7242807667195592
1107	18-19	0.8227474441822977
1107	20-21	0.5672771027878127
1107	22-23	0.89770015222701
1107	24-25	0.5757929304602314
1107	26-27	0.5260586520219235
1107	28-29	0.6684859649857771
1107	30-31	0.6869384826900458
1107	32-33	0.6377205432709729
1107	34-35	0.48473555099774757
1107	36-37	0.4866600129655012
1107	38-39	0.505601133869348
1107	40-41	0.401443326279157
1107	42-43	0.5058782575001644
1107	44-45	0.47836677454893817
1107	46-47	0.48545584834184297
1107	48-49	0.4819221929135651
1107	50-51	0.5302493218946225
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2216	90-91	-1.4408806572297834
2216	92-93	-1.3878325449553124
2216	94-95	-1.2766538109224896
2216	96-97	-1.3001436265354585
2216	98-99	-1.2915843677437202
2216	100	-1.271401419437602
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	23514.0
1	3865.0
2	593.0
3	598.0
4	570.0
5	309.0
6	292.0
7	390.0
8	661.0
9	1093.0
10	2043.0
11	4267.0
12	7632.0
13	11336.0
14	14734.0
15	18500.0
16	22079.0
17	26012.0
18	30107.0
19	33944.0
20	38213.0
21	43598.0
22	49874.0
23	57103.0
24	64819.0
25	72943.0
26	82556.0
27	93962.0
28	111190.0
29	131285.0
30	154406.0
31	183785.0
32	224031.0
33	282663.0
34	382336.0
35	513672.0
36	746840.0
37	1111118.0
38	887173.0
39	283575.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.700461594718774	21.033332685605064	12.582333623633367	23.683872096042794
2	15.265397760661104	19.84289316893596	40.94302747305353	23.948681597349406
3	18.580756680458492	26.956530449562838	28.830886374212522	25.63182649576615
4	12.261374220921809	16.888550900068015	40.43116885581393	30.41890602319624
5	13.188020940554374	38.6120278205003	34.97799429060043	13.221956948344898
6	29.50153082179322	38.5801889287692	18.476925020926767	13.441355228510806
7	26.71734431186878	31.723740684480422	24.012341360643273	17.54657364300753
8	25.940404046795912	35.007252536630055	20.19453532087289	18.857808095701138
9	26.00541858298658	15.22646079965852	21.903455274011847	36.864665343343056
10-11	25.5754082906759	25.83103199907375	27.607140725260294	20.986418984990053
12-13	25.92204283043903	23.59228419484378	27.55355343334587	22.932119541371325
14-15	23.348305746672743	24.836750293287295	25.03575050119271	26.779193458847256
16-17	22.292044329648572	27.388424215677137	26.398180887894096	23.92135056678019
18-19	21.68908621230471	28.190991812144922	27.928088309554656	22.191833665995716
20-21	23.492336938404346	27.243824399424426	26.797331150317255	22.466507511853976
22-23	24.026602986258236	26.778225138857103	26.510467581378293	22.684704293506364
24-25	23.45367533443319	26.922472719958723	27.14641129077985	22.47744065482824
26-27	23.269598426669553	27.178534614535447	26.184480307353507	23.367386651441493
28-29	22.311944065463926	27.669931670999087	26.91010136754688	23.108022895990107
30-31	23.165144953065262	27.249063168694327	26.824324704429635	22.76146717381077
32-33	23.568388098575237	27.486433400710336	25.815694666580523	23.1294838341339
34-35	23.53502725836329	27.2483677201691	26.759261790792664	22.457343230674947
36-37	23.251194640441934	26.878491090821516	26.39634400369018	23.47397026504637
38-39	23.431753571794374	26.88664726307863	27.027017759953793	22.654581405173204
40-41	23.032999883501375	26.04362323905737	27.681752139288392	23.24162473815286
42-43	23.045967314878695	26.30071095858121	26.959461829772092	23.693859896768004
44-45	23.155796643004507	27.212571406563896	26.764649012563304	22.8669829378683
46-47	23.11670739667002	26.91583059003359	26.318047850932146	23.649414162364245
48-49	23.84514315837571	26.4479491973836	26.721012733090504	22.985894911150183
50-51	23.413353083090616	26.392899019782902	26.599214704496106	23.59453319263038
52-53	22.90869409692189	26.9628993808549	26.41532224353662	23.71308427868659
54-55	22.84533656993068	26.89273906428517	26.41816023003169	23.84376413575246
56-57	23.933072305890875	26.304542369782236	26.16402181518976	23.598363509137133
58-59	22.74001696023558	26.71861288271081	27.18130062817576	23.36006952887785
60-61	22.844368687989725	27.33367412016999	26.42727197207013	23.39468521977015
62-63	23.37824968456236	26.58229973335696	25.944919673938475	24.094530908142204
64-65	22.439422055446133	26.812318526387674	26.475542886761872	24.27271653140432
66-67	23.3007945396716	26.348625159778255	26.432509841637113	23.918070458913032
68-69	22.52296755534398	26.515183356192	26.782664083423036	24.17918500504098
70-71	22.53146885115616	26.14615367032565	26.91851418142305	24.403863297095143
72-73	23.163686089738384	26.272516974219446	27.233498512612815	23.33029842342936
74-75	23.322444453216427	26.63213417332177	26.762573913581672	23.28284745988013
76-77	23.029463462865817	26.553825478718863	26.366632946518582	24.05007811189674
78-79	22.923305139516746	26.608643504885045	26.39925187756046	24.068799478037754
80-81	22.49342285881715	26.914091595860988	27.14885292213897	23.443632623182886
82-83	23.362938453928244	25.83464999067725	28.16276214359815	22.639649411796352
84-85	23.601240536234062	26.396078012131767	26.738591698085074	23.2640897535491
86-87	22.242307821878104	27.062989902503215	27.247658265814145	23.447044009804532
88-89	23.295124396798155	25.671685054866593	27.12562135172923	23.907569196606023
90-91	22.949655872449988	25.83384616472852	27.26051658036952	23.95598138245197
92-93	23.20457630892207	26.12168499926523	27.24015595072772	23.433582741084972
94-95	22.080026795973033	26.074177733062804	27.89222579002759	23.95356968093657
96-97	22.472791106841143	26.009980374000097	27.377593468857686	24.139635050301074
98-99	21.899722990661115	26.701725103928236	27.22780501463735	24.1707468907733
100	22.714796447114196	25.822271896940634	26.55565180474299	24.907279851202173
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19580.0
1	11456.0
2	3102.5
3	2226.5
4	1143.0
5	587.0
6	427.0
7	382.0
8	382.5
9	389.0
10	364.0
11	316.0
12	302.0
13	338.5
14	355.5
15	401.0
16	460.5
17	532.0
18	663.5
19	801.5
20	1046.0
21	1388.0
22	1980.5
23	2837.5
24	3838.5
25	5201.0
26	7490.0
27	10448.0
28	14994.5
29	25031.0
30	39098.0
31	53063.5
32	71893.0
33	91413.5
34	108570.5
35	125554.0
36	141830.5
37	170339.0
38	183048.5
39	172912.5
40	162216.5
41	152443.0
42	151929.0
43	162005.5
44	178780.0
45	211128.5
46	243264.5
47	265906.5
48	279456.0
49	273383.5
50	261420.0
51	265780.5
52	268042.0
53	256128.0
54	241486.5
55	208816.5
56	181454.5
57	150757.0
58	118764.5
59	110210.5
60	90614.5
61	63298.5
62	47169.0
63	38338.0
64	27795.5
65	13932.0
66	7649.0
67	5226.0
68	3742.0
69	2710.0
70	1954.5
71	1471.0
72	1113.0
73	868.5
74	653.5
75	440.0
76	294.0
77	199.0
78	139.5
79	100.5
80	71.5
81	56.0
82	44.0
83	31.5
84	23.5
85	22.5
86	20.5
87	16.5
88	17.0
89	15.0
90	12.0
91	9.5
92	7.0
93	5.5
94	5.5
95	5.0
96	3.0
97	1.0
98	2.0
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9977682213680955
2	0.4469503543370502
3	0.4815262210816247
4	0.46660805500518465
5	0.4336062034386635
6	0.48584601829250484
7	0.5019184621661842
8	0.5039821709551876
9	0.5186904683412206
10-11	0.5316586045026278
12-13	0.5306267501081261
14-15	0.5277935227876298
16-17	0.5329090721163037
18-19	0.5318947067793358
20-21	0.5302157572560788
22-23	0.49317393339922044
24-25	0.5271289386013406
26-27	0.5078297636126516
28-29	0.5133737948509066
30-31	0.5165742923796154
32-33	0.5197485563220232
34-35	0.5306267501081261
36-37	0.5495411658310687
38-39	0.5381120667326471
40-41	0.5438834557188431
42-43	0.5414874548366951
44-45	0.5508441006173462
46-47	0.549689822820107
48-49	0.5213050824425427
50-51	0.5369577889354079
52-53	0.5292276255054119
54-55	0.5389865196093435
56-57	0.5260883396780719
58-59	0.5323669113327519
60-61	0.5254325000205496
62-63	0.5199846585987312
64-65	0.5315799037437252
66-67	0.5297610417601967
68-69	0.5503806405926972
70-71	0.5332588532669822
72-73	0.5370364896943105
74-75	0.5344743427655901
76-77	0.5223456813658114
78-79	0.5531089335679898
80-81	0.5276361212698245
82-83	0.527513697867087
84-85	0.5359171900121392
86-87	0.8436371573215927
88-89	1.1115257960975267
90-91	1.1014783325442854
92-93	1.1017756465223623
94-95	1.1067075607469297
96-97	1.0994058792265151
98-99	1.0954533522238474
100	1.0913521682321414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5717861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.112698481872155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51790532675973	20.667867054700192
2	12.429348041395837	6.988450276280187
3	5.139470686743648	4.334531693185338
4	2.658226498772869	2.9891968022609747
5	1.6536970544071483	2.324494333545415
6	1.0129510509785145	1.7086072483832693
7	0.6410757417434431	1.2615658322173169
8	0.46278307307906486	1.0408064796788765
9	0.33897407655641937	0.85765284066615
>10	1.6788795151859517	8.558257935780146
>50	0.1722729534998116	3.4355191118571304
>100	0.21160334820630972	13.330411972294687
>500	0.04354962909061279	8.486128805939162
>1k	0.03684012835967854	18.474790327191684
>5k	0.001925875175632436	3.4908199779805376
>10k+	4.970000453244996E-4	2.050899308038907
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	20583	0.3599772712208289	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17547	0.3068804925478251	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16407	0.28694296695914784	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15160	0.2651341122143403	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13350	0.23347891807793159	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12312	0.2153252763577149	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11980	0.20951890925645097	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10090	0.17646459051732807	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9377	0.1639948924956378	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9327	0.16312043961894143	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8921	0.15601988226016686	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7850	0.13728910164133057	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7787	0.13618729101669313	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7397	0.1293665585784614	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7396	0.12934906952092748	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7191	0.12576381272647236	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6820	0.11927537238138528	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6802	0.11896056934577459	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6783	0.11862827725262995	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6619	0.11576007181706585	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6482	0.1133640709349178	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6348	0.11102053722537153	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6143	0.1074352804309164	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5966	0.10433971724741122	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5954	0.10412984855700409	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5901	0.10320292850770595	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5901	0.10320292850770595	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.748905753392746E-5	0.0
6	0.0	0.0	0.0	1.748905753392746E-5	0.0
7	0.0	0.0	0.0	1.748905753392746E-5	0.0
8	0.0	0.0	0.0	1.748905753392746E-5	0.0
9	0.0	0.0	0.0	1.748905753392746E-5	0.0
10-11	0.0	0.0	0.0	1.748905753392746E-5	0.0
12-13	0.0	0.0	0.0	1.748905753392746E-5	3.497811506785492E-5
14-15	0.0	0.0	0.0	1.748905753392746E-5	4.372264383481865E-5
16-17	0.0	0.0	0.0	1.748905753392746E-5	5.246717260178238E-5
18-19	0.0	0.0	0.0	2.623358630089119E-5	6.995623013570984E-5
20-21	0.0	0.0	0.0	4.372264383481865E-5	8.74452876696373E-5
22-23	0.0	0.0	0.0	9.618981643660102E-5	8.74452876696373E-5
24-25	0.0	0.0	0.0	2.27357747941057E-4	8.74452876696373E-5
26-27	0.0	0.0	0.0	4.896936109499688E-4	8.74452876696373E-5
28-29	0.0	0.0	0.0	0.0015390370629856165	8.74452876696373E-5
30-31	0.0	0.0	0.0	0.006243593539612103	8.74452876696373E-5
32-33	0.0	0.0	0.0	0.01768143716680066	8.74452876696373E-5
34-35	0.0	0.0	0.0	0.03465456750347726	8.74452876696373E-5
36-37	0.0	0.0	0.0	0.06601244766180919	8.74452876696373E-5
38-39	0.0	0.0	0.0	0.11451834873215701	8.74452876696373E-5
40-41	0.0	0.0	0.0	0.1701422962188133	8.74452876696373E-5
42-43	0.0	0.0	0.0	0.23035712130812555	8.74452876696373E-5
44-45	0.0	0.0	0.0	0.3031465787643316	8.74452876696373E-5
46-47	0.0	0.0	0.0	0.40042071676803614	8.74452876696373E-5
48-49	0.0	0.0	0.0	0.5151839123056682	8.74452876696373E-5
50-51	0.0	0.0	0.0	0.6393212426814853	1.0493434520356476E-4
52-53	0.0	0.0	0.0	0.7643067923476978	1.0493434520356476E-4
54-55	0.0	8.74452876696373E-6	0.0	0.9033185661561203	1.0493434520356476E-4
56-57	0.0	1.748905753392746E-5	0.0	1.0724727306242667	1.0493434520356476E-4
58-59	0.0	1.748905753392746E-5	0.0	1.2764738422287634	1.0493434520356476E-4
60-61	0.0	1.748905753392746E-5	0.0	1.4915542717810033	1.0493434520356476E-4
62-63	0.0	1.748905753392746E-5	0.0	1.7248845328698965	1.0493434520356476E-4
64-65	0.0	1.748905753392746E-5	0.0	1.9673528265202669	1.224234027374922E-4
66-67	0.0	1.748905753392746E-5	0.0	2.249879806452098	1.3991246027141968E-4
68-69	0.0	1.748905753392746E-5	0.0	2.5639220680600667	1.3991246027141968E-4
70-71	0.0	1.748905753392746E-5	0.0	2.9103453196921016	1.486569890383834E-4
72-73	0.0	1.748905753392746E-5	0.0	3.2787173385292157	1.5740151780534715E-4
74-75	0.0	1.748905753392746E-5	0.0	3.6413791800815023	1.5740151780534715E-4
76-77	0.0	1.748905753392746E-5	0.0	4.064754284862818	1.5740151780534715E-4
78-79	0.0	1.748905753392746E-5	0.0	4.518455415407965	1.5740151780534715E-4
80-81	0.0	1.748905753392746E-5	0.0	5.008227727116836	1.5740151780534715E-4
82-83	0.0	1.748905753392746E-5	0.0	5.5208582370225505	1.5740151780534715E-4
84-85	0.0	1.748905753392746E-5	0.0	6.033777316377575	1.5740151780534715E-4
86-87	0.0	1.748905753392746E-5	0.0	6.587087374107206	1.5740151780534715E-4
88	0.0	1.748905753392746E-5	0.0	7.026578645406036	1.5740151780534715E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5990	0.0	39.332024	94
GTACTAG	5960	0.0	33.086388	1
GTATCAA	11895	0.0	31.96612	1
CAACGCA	10555	0.0	31.736715	5
TAGGCAT	7380	0.0	30.748323	5
ATCAACG	11355	0.0	29.588188	3
TAGTACT	8690	0.0	29.196028	4
GCATCGT	6440	0.0	29.013952	8
CTGTGCG	8855	0.0	28.896698	9
AGGGCTA	6370	0.0	28.24815	5
CTATTGA	6295	0.0	28.04351	9
TATTCTC	2975	0.0	27.478497	5
CTAGGCA	8460	0.0	27.324017	4
TATCAAC	13935	0.0	27.044699	2
GCTATTG	6455	0.0	26.982822	8
GGTATCA	7950	0.0	26.584606	1
ACTGTGC	9835	0.0	26.492828	8
CTAGTAC	10305	0.0	25.9912	3
ACGCAGA	12890	0.0	25.822102	7
GTACTGT	10010	0.0	25.793352	6
>>END_MODULE