##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5717861
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81272314944347	31.0	31.0	34.0	30.0	34.0
2	31.781620084853408	31.0	31.0	34.0	30.0	34.0
3	31.984075338662482	33.0	31.0	34.0	30.0	34.0
4	35.728704492816455	37.0	35.0	37.0	35.0	37.0
5	35.62215993008574	37.0	35.0	37.0	33.0	37.0
6	35.66236272620129	37.0	35.0	37.0	33.0	37.0
7	35.58907378126191	37.0	35.0	37.0	33.0	37.0
8	35.579336748479896	37.0	35.0	37.0	33.0	37.0
9	37.162526336334516	39.0	37.0	39.0	33.0	39.0
10-11	37.10844658518282	39.0	37.0	39.0	33.0	39.0
12-13	37.037663035180465	39.0	37.0	39.0	33.0	39.0
14-15	38.23966584707114	40.0	37.0	41.0	33.0	41.0
16-17	38.238231569462776	40.0	37.0	41.0	33.0	41.0
18-19	38.28835232266052	40.0	38.0	41.0	33.0	41.0
20-21	38.299259460836836	40.0	38.0	41.0	33.0	41.0
22-23	38.265446466781896	40.0	37.5	41.0	33.0	41.0
24-25	38.19588889971267	40.0	37.0	41.0	33.0	41.0
26-27	38.054897801817845	40.0	37.0	41.0	33.0	41.0
28-29	37.98985678385676	40.0	37.0	41.0	33.0	41.0
30-31	37.87665308757943	40.0	37.0	41.0	33.0	41.0
32-33	37.76458172033213	40.0	36.5	41.0	32.5	41.0
34-35	37.67584434109189	40.0	36.0	41.0	32.0	41.0
36-37	37.600164641987625	40.0	36.0	41.0	32.0	41.0
38-39	37.4850092543348	40.0	36.0	41.0	32.0	41.0
40-41	37.367327397430614	40.0	36.0	41.0	31.0	41.0
42-43	37.24823251212298	39.0	36.0	41.0	31.0	41.0
44-45	37.05075394452576	39.0	35.0	41.0	31.0	41.0
46-47	36.82964145158478	39.0	35.0	41.0	30.5	41.0
48-49	36.585638930362244	39.0	35.0	41.0	30.0	41.0
50-51	36.38141369648545	39.0	35.0	41.0	30.0	41.0
52-53	36.18390452303755	38.0	35.0	40.5	29.0	41.0
54-55	35.98191823830625	38.0	35.0	40.0	29.0	41.0
56-57	35.71328124275844	37.5	34.0	40.0	29.0	41.0
58-59	35.42877257771744	37.0	34.0	40.0	28.0	41.0
60-61	35.17145878502468	36.5	34.0	40.0	28.0	41.0
62-63	34.88925605221953	36.0	34.0	39.5	28.0	41.0
64-65	34.61597772313807	35.5	33.5	39.0	28.0	41.0
66-67	34.38821239271119	35.0	33.0	39.0	27.5	41.0
68-69	34.189037036752026	35.0	33.0	38.5	27.0	41.0
70-71	33.984831128283815	35.0	33.0	37.5	27.0	40.0
72-73	33.78253345088312	35.0	33.0	37.0	27.0	40.0
74-75	33.60399920529723	35.0	33.0	37.0	27.0	39.0
76-77	33.44650586993983	35.0	33.0	36.0	27.0	39.0
78-79	33.3320326429761	35.0	33.0	36.0	27.0	39.0
80-81	33.188767093148996	35.0	33.0	36.0	27.0	38.0
82-83	33.001720048808465	35.0	33.0	35.0	27.0	37.0
84-85	32.863678830247885	35.0	33.0	35.0	27.0	37.0
86-87	32.73321465841859	35.0	33.0	35.0	27.0	36.0
88-89	32.62840981968607	35.0	33.0	35.0	27.0	36.0
90-91	32.53667236751645	35.0	33.0	35.0	27.0	36.0
92-93	32.45983629192804	35.0	33.0	35.0	27.0	36.0
94-95	32.42117227053963	35.0	33.0	35.0	27.0	35.0
96-97	32.37202828120516	35.0	33.0	35.0	26.5	35.0
98-99	32.33410649541848	35.0	33.0	35.0	26.0	35.0
100	31.833115740309182	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4594138218612933
1101	2	0.5580295478106834
1101	3	0.4729844838632147
1101	4	0.3711249864348858
1101	5	0.36467156722388694
1101	6	0.40297483839626835
1101	7	0.3973804624232713
1101	8	0.38390792589400036
1101	9	0.4930243088185904
1101	10-11	0.5290792513612104
1101	12-13	0.49806110209198806
1101	14-15	0.6522873171852908
1101	16-17	0.6331723265920957
1101	18-19	0.6249349597554428
1101	20-21	0.6050225572293186
1101	22-23	0.6431074861626627
1101	24-25	0.6988899994944262
1101	26-27	0.6480431284881192
1101	28-29	0.7281903151371125
1101	30-31	0.7011831601170186
1101	32-33	0.759562654189736
1101	34-35	0.7051661042665387
1101	36-37	0.7235623701486844
1101	38-39	0.7605641742459284
1101	40-41	0.761135491012638
1101	42-43	0.7246623498012212
1101	44-45	0.6558469025612013
1101	46-47	0.7012008809753496
1101	48-49	0.7322404615699298
1101	50-51	0.6941646032301492
1101	52-53	0.7155515889005954
1101	54-55	0.7358746312032167
1101	56-57	0.7428911552151902
1101	58-59	0.7548216227169888
1101	60-61	0.700293516464157
1101	62-63	0.7088900727007683
1101	64-65	0.7312752466536097
1101	66-67	0.6161302703524711
1101	68-69	0.6459793290776545
1101	70-71	0.6525926594776621
1101	72-73	0.6142946723477394
1101	74-75	0.5283699175648664
1101	76-77	0.5837570590857908
1101	78-79	0.5687430989645748
1101	80-81	0.5477037812664207
1101	82-83	0.5406257557599474
1101	84-85	0.5639342968381413
1101	86-87	0.5549126807576243
1101	88-89	0.5310071740983062
1101	90-91	0.5383623493758165
1101	92-93	0.5303116934676027
1101	94-95	0.48587904155042594
1101	96-97	0.559844696579411
1101	98-99	0.5735796051288489
1101	100	0.5848174658679994
1102	1	0.41007256316620655
1102	2	0.548335397497361
1102	3	0.4524141452197341
1102	4	0.33672027525436476
1102	5	0.35707249497746574
1102	6	0.3371269690576071
1102	7	0.33631990103189224
1102	8	0.3617840170097679
1102	9	0.42423694634593545
1102	10-11	0.452247268029744
1102	12-13	0.4888652503187316
1102	14-15	0.58503836528363
1102	16-17	0.6338929019202268
1102	18-19	0.5982300536076508
1102	20-21	0.6003648706367954
1102	22-23	0.5586807619189926
1102	24-25	0.5874315809922734
1102	26-27	0.6041227686913331
1102	28-29	0.602056081132524
1102	30-31	0.622112598884577
1102	32-33	0.6352873885092123
1102	34-35	0.6480625613678228
1102	36-37	0.6160519757920184
1102	38-39	0.6691607176435141
1102	40-41	0.532561658374199
1102	42-43	0.5756787214381234
1102	44-45	0.5314946874115236
1102	46-47	0.6051335334368204
1102	48-49	0.5705570426118811
1102	50-51	0.4906419655313812
1102	52-53	0.4693699981948214
1102	54-55	0.49340526706831156
1102	56-57	0.5713272410199366
1102	58-59	0.5109424808331227
1102	60-61	0.46967972946574577
1102	62-63	0.44699414782030544
1102	64-65	0.49757574822211836
1102	66-67	0.4457615572678293
1102	68-69	0.46684171931183016
1102	70-71	0.4436291766312692
1102	72-73	0.4284702434614758
1102	74-75	0.42579944239981415
1102	76-77	0.39698601255401655
1102	78-79	0.3681113992327525
1102	80-81	0.414498097858754
1102	82-83	0.4330856989177505
1102	84-85	0.4718301005973302
1102	86-87	0.44898657269502706
1102	88-89	0.44827704517361155
1102	90-91	0.3988502043276867
1102	92-93	0.4029742720484748
1102	94-95	0.446676044506475
1102	96-97	0.47211918007041476
1102	98-99	0.4446385689992951
1102	100	0.5087808416667059
1103	1	0.2833182089941353
1103	2	-0.1748544286515532
1103	3	0.2034470424822885
1103	4	0.21202783326965147
1103	5	0.24892054455091284
1103	6	0.2883843879054169
1103	7	0.3381650146199746
1103	8	0.34446287591931224
1103	9	0.38974654434471034
1103	10-11	0.40665278727574616
1103	12-13	0.39724060713552944
1103	14-15	0.5635554428015084
1103	16-17	0.5187235087494244
1103	18-19	0.5508692003709115
1103	20-21	0.5004884748907799
1103	22-23	0.47344882428695456
1103	24-25	0.48366417737104683
1103	26-27	0.47885284199352185
1103	28-29	0.5498127061334586
1103	30-31	0.6073880708113961
1103	32-33	0.6029348843831173
1103	34-35	0.5731803499319597
1103	36-37	0.5637853458503557
1103	38-39	0.530246015261703
1103	40-41	0.5589560507880833
1103	42-43	0.534322594022612
1103	44-45	0.5636594192756519
1103	46-47	0.5461872176216147
1103	48-49	0.5254728808250206
1103	50-51	0.5150576880300051
1103	52-53	0.5780814286674456
1103	54-55	0.5822066103988632
1103	56-57	0.49408871764026685
1103	58-59	0.4538193460032858
1103	60-61	0.502487437693631
1103	62-63	0.5178295367516057
1103	64-65	0.507102379730263
1103	66-67	0.5318417661573989
1103	68-69	0.4705597612232566
1103	70-71	0.46016850218529726
1103	72-73	0.3871654853673334
1103	74-75	0.4338741186714614
1103	76-77	0.4165858123191981
1103	78-79	0.3910598977034496
1103	80-81	0.3950917961523288
1103	82-83	0.37838489531552
1103	84-85	0.3941931946464692
1103	86-87	0.46318650313604337
1103	88-89	0.48249946164589375
1103	90-91	0.42493623099157674
1103	92-93	0.42257381125407534
1103	94-95	0.4346596424371896
1103	96-97	0.4022672016232747
1103	98-99	0.4177447424947758
1103	100	0.46412042141223964
1104	1	0.34682791044385297
1104	2	0.46203575249563045
1104	3	0.338577553258645
1104	4	0.2673340951300247
1104	5	0.2944463515868563
1104	6	0.2870477971105885
1104	7	0.331235611338208
1104	8	0.3284477122950733
1104	9	0.32533508388661403
1104	10-11	0.35693620769857404
1104	12-13	0.36935572335918465
1104	14-15	0.5031141777955597
1104	16-17	0.46034316368172057
1104	18-19	0.4785909676812068
1104	20-21	0.4902932835680147
1104	22-23	0.5070913800128878
1104	24-25	0.47752167011678637
1104	26-27	0.5069994417939157
1104	28-29	0.5268954928691656
1104	30-31	0.5408843759808946
1104	32-33	0.5253565411482555
1104	34-35	0.5417746563876591
1104	36-37	0.5806719643918044
1104	38-39	0.5921121614029659
1104	40-41	0.5662945809951552
1104	42-43	0.5265060786065661
1104	44-45	0.4477755890824966
1104	46-47	0.48053622127962825
1104	48-49	0.5032036259315191
1104	50-51	0.5161113827886012
1104	52-53	0.44867606051556663
1104	54-55	0.45299993890206025
1104	56-57	0.43083863094911834
1104	58-59	0.4116094412601541
1104	60-61	0.4421699366043157
1104	62-63	0.45949208477264847
1104	64-65	0.35363465201916
1104	66-67	0.34977967075018057
1104	68-69	0.35611285570175255
1104	70-71	0.3854543139598121
1104	72-73	0.3542205993664993
1104	74-75	0.3847976192691718
1104	76-77	0.3676383640885561
1104	78-79	0.32429916046000784
1104	80-81	0.30641565960077344
1104	82-83	0.3024575888859573
1104	84-85	0.3504617815482902
1104	86-87	0.2835981054636534
1104	88-89	0.31027694162386865
1104	90-91	0.29355293978851194
1104	92-93	0.2869639516156255
1104	94-95	0.32503559986424335
1104	96-97	0.33615194957976513
1104	98-99	0.31075367233998463
1104	100	0.35522703037439385
1105	1	0.13462582553042068
1105	2	-0.3672020246280141
1105	3	0.015151016366932168
1105	4	0.08432980496655773
1105	5	0.18128092272051788
1105	6	0.2138947317435509
1105	7	0.2366549634521462
1105	8	0.20071725848782762
1105	9	0.2340161822224971
1105	10-11	0.3030294885846132
1105	12-13	0.27302033003091
1105	14-15	0.3587033454568669
1105	16-17	0.3680137181727261
1105	18-19	0.37823785090304085
1105	20-21	0.3999209965500725
1105	22-23	0.35552310031202694
1105	24-25	0.41002294611438117
1105	26-27	0.3932221791551811
1105	28-29	0.391992140368437
1105	30-31	0.40515985124930154
1105	32-33	0.46169756730530764
1105	34-35	0.48170370197553325
1105	36-37	0.46881829208989956
1105	38-39	0.44135788509944973
1105	40-41	0.4208161827657193
1105	42-43	0.4468567660143279
1105	44-45	0.4159474227356199
1105	46-47	0.47256389419411704
1105	48-49	0.48192746524230756
1105	50-51	0.46698277641044683
1105	52-53	0.47287823111420835
1105	54-55	0.48522539129165665
1105	56-57	0.44399956255911377
1105	58-59	0.4944756187949224
1105	60-61	0.49507631741994373
1105	62-63	0.4468247011820523
1105	64-65	0.49637195121042055
1105	66-67	0.5085660407894181
1105	68-69	0.48448775599685234
1105	70-71	0.45113641416757133
1105	72-73	0.39712039617450046
1105	74-75	0.4293383669371238
1105	76-77	0.4057478587095247
1105	78-79	0.3182919579122867
1105	80-81	0.3706790245307303
1105	82-83	0.2969509267088455
1105	84-85	0.3433953686347948
1105	86-87	0.3637907280036927
1105	88-89	0.3816324128587354
1105	90-91	0.3267648735716193
1105	92-93	0.35422413846415424
1105	94-95	0.3350635808316582
1105	96-97	0.3489889752782105
1105	98-99	0.3858842342639406
1105	100	0.41516205688721186
1106	1	0.2933682024651354
1106	2	0.34229500463756324
1106	3	0.3134091045961256
1106	4	0.21691863705798653
1106	5	0.23113435458105158
1106	6	0.20664963414088788
1106	7	0.24558930540371193
1106	8	0.21971150570092846
1106	9	0.24444358960921875
1106	10-11	0.2921753643871057
1106	12-13	0.32073181922711314
1106	14-15	0.40335066492786353
1106	16-17	0.3944439022030437
1106	18-19	0.4348024481815429
1106	20-21	0.42682823144627235
1106	22-23	0.38690546856233254
1106	24-25	0.3449744724633135
1106	26-27	0.3859162442021429
1106	28-29	0.39997519823869965
1106	30-31	0.36953303219805633
1106	32-33	0.36390900782156166
1106	34-35	0.35057938774008335
1106	36-37	0.3695194153512844
1106	38-39	0.3039238829011808
1106	40-41	0.3644129966068306
1106	42-43	0.3595646589325412
1106	44-45	0.41057757843848464
1106	46-47	0.4341798469260212
1106	48-49	0.43498065996735846
1106	50-51	0.47247767910464944
1106	52-53	0.4754246953025003
1106	54-55	0.3659241962808153
1106	56-57	0.4091613145641162
1106	58-59	0.3495836354381723
1106	60-61	0.36742839894664314
1106	62-63	0.4469790430179046
1106	64-65	0.3967655920752122
1106	66-67	0.38100563929435793
1106	68-69	0.3451387074696086
1106	70-71	0.4199833133558073
1106	72-73	0.4287461578520819
1106	74-75	0.3922633635974506
1106	76-77	0.331598474680618
1106	78-79	0.34256268959322256
1106	80-81	0.36038194605689
1106	82-83	0.3741330774969214
1106	84-85	0.3623178726807623
1106	86-87	0.29675526988517476
1106	88-89	0.2727968836448653
1106	90-91	0.27063076674291864
1106	92-93	0.3179155863666736
1106	94-95	0.3785922301732967
1106	96-97	0.3178781077764583
1106	98-99	0.27076541369217466
1106	100	0.3930586833294072
1107	1	0.04478363453858947
1107	2	-0.604445660269679
1107	3	-0.0783095720178828
1107	4	0.01944263838513649
1107	5	0.06626695197231669
1107	6	0.12698977791980326
1107	7	0.13954451113720978
1107	8	0.1921403758740965
1107	9	0.20079349688424486
1107	10-11	0.22491455419596207
1107	12-13	0.16613648447921037
1107	14-15	0.22744916292895567
1107	16-17	0.3278579333761158
1107	18-19	0.3123232840776353
1107	20-21	0.2210514249013471
1107	22-23	0.21881358815209495
1107	24-25	0.20960563929001808
1107	26-27	0.28511827395313816
1107	28-29	0.1854967043200091
1107	30-31	0.21221736054315699
1107	32-33	0.2574590158496761
1107	34-35	0.2770758443347816
1107	36-37	0.28674803051762154
1107	38-39	0.3336186114333657
1107	40-41	0.34183085648705
1107	42-43	0.3236160637305687
1107	44-45	0.38279446235961245
1107	46-47	0.3381195657035647
1107	48-49	0.3562766475225203
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2216	76-77	-0.6512689326076568
2216	78-79	-0.6097400885337692
2216	80-81	-0.5611302133551419
2216	82-83	-0.5998378866526082
2216	84-85	-0.5904084756422208
2216	86-87	-0.4813505486859384
2216	88-89	-0.475959333950307
2216	90-91	-0.5902626109958717
2216	92-93	-0.5550096734981906
2216	94-95	-0.5556447102138762
2216	96-97	-0.5791295746861209
2216	98-99	-0.6007809019634891
2216	100	-0.7227241349329248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	10.0
13	71.0
14	279.0
15	847.0
16	2154.0
17	4438.0
18	8069.0
19	12297.0
20	18059.0
21	26083.0
22	35720.0
23	42551.0
24	45133.0
25	49322.0
26	56626.0
27	70466.0
28	88978.0
29	111711.0
30	138402.0
31	170077.0
32	212263.0
33	268448.0
34	366240.0
35	504522.0
36	747897.0
37	1271561.0
38	1077311.0
39	387656.0
40	670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69865762009835	21.826402790932793	13.8369466257717	24.637992963197153
2	14.790919851910306	20.406949329930516	39.90393898435992	24.898191833799263
3	17.8899235801464	26.612385455480343	29.81895866472068	25.678732299652573
4	12.424526568991908	17.44234419418325	39.021596326262845	31.111532910561994
5	12.793434394224926	38.6741638865448	35.04581844893247	13.486583270297805
6	29.35728937796844	39.065745039972114	17.90118717471446	13.675778407344986
7	25.899313918162324	32.32540451090632	24.04359275396562	17.731688816965736
8	25.987725130079237	35.027644078791006	19.827134657523153	19.157496133606607
9	25.905386647209504	14.847282926255115	21.694703666283598	37.552626760251776
10-11	25.539113315276463	25.967507779570013	27.34810272582702	21.1452761793265
12-13	25.961404098490675	23.685701698589735	27.791975006038093	22.56091919688149
14-15	23.282128115598198	24.712771943362984	25.548233167247385	26.456866773791432
16-17	21.8800790737396	27.24769098628977	26.860411203733808	24.01181873623682
18-19	21.343873172153014	27.39141437680979	29.237891582184318	22.02682086885288
20-21	22.88765303370598	26.551822378240242	28.44864283093424	22.111881757119537
22-23	23.326593293125114	26.051478190243678	28.22749126655712	22.39443725007409
24-25	22.851639800267968	26.17530401665938	28.879164778577167	22.09389140449549
26-27	22.686243720433055	26.463685960309736	27.897493997088592	22.952576322168618
28-29	21.844882203327433	27.010327813145512	28.52484521746856	22.6199447660585
30-31	22.78074790555419	26.504063320182148	28.383979603561542	22.33120917070212
32-33	23.20516360925878	26.735968223082025	27.53932808090298	22.51954008675622
34-35	23.040984918242657	26.582124178119866	28.340788078005048	22.03610282563243
36-37	22.858346853832227	26.127086685038336	28.00068067411922	23.013885787010214
38-39	23.002955126051507	26.140736894443567	28.667109955978294	22.18919802352663
40-41	22.53306010675704	25.448180371514223	29.145649854324436	22.873109667404297
42-43	22.753923824557827	25.6415855184146	28.281828044115358	23.32266261291221
44-45	22.896866503050703	26.531048935956996	27.944418376032576	22.627666184959725
46-47	22.942477965099187	26.377731113085822	27.247138396683656	23.43265252513134
48-49	23.832923854409255	26.049759034711734	27.34148898289493	22.77582812798408
50-51	23.184465309667374	26.188936737007072	27.034077953276586	23.59252000004897
52-53	22.600654335598573	26.653192513773945	26.989725703370542	23.756427447256936
54-55	22.664992026278437	26.602857073150552	26.806255399215562	23.92589550135545
56-57	23.733509226753068	26.023843025791223	26.622791250532433	23.619856496923276
58-59	22.497689035012204	26.49332585616323	27.57883011786162	23.430154990962947
60-61	22.72053148583464	27.085829533687182	26.76159966924671	23.432039311231463
62-63	23.26001803821394	26.321293924423838	26.2642358742194	24.154452163142825
64-65	22.270478418415557	26.46309520290892	26.85861023903869	24.407816139636832
66-67	23.030238055804432	26.05198867198765	26.85521736187711	24.062555910330804
68-69	22.450396224202535	26.02650099413488	27.187833471542433	24.33526931012015
70-71	22.416658162553695	25.733953044879065	27.28292584859461	24.56646294397263
72-73	23.00542982769256	25.834555964197104	27.52870347845112	23.631310729659223
74-75	23.094307904692506	26.313559614753828	27.04919444815845	23.542938032395217
76-77	22.807186762734325	26.260494483233792	26.53860424630051	24.393714507731378
78-79	22.563129226359276	26.39540140630151	26.701574984237915	24.339894383101296
80-81	22.192531723686038	26.858846791576635	27.277433558631138	23.671187926106192
82-83	23.0993531992219	25.699909448765535	28.28190947237619	22.91882787963638
84-85	23.42386406188812	26.251188666180557	26.721967810298935	23.602979461632383
86-87	21.980575674143846	26.929749851265917	27.212097557665228	23.87757691692501
88-89	22.86152319379638	25.732067917181027	27.083504593144408	24.322904295878185
90-91	22.48410745288379	25.768457877458978	27.25752524447365	24.48990942518358
92-93	22.786756446748843	26.147632708559765	26.997838869495773	24.067771975195623
94-95	21.664508485639004	25.989685153918185	27.6860871478396	24.659719212603218
96-97	22.015744883323933	25.969219446574076	27.18088094076478	24.834154729337214
98-99	21.422366443601184	26.793007672769352	27.010566913261208	24.774058970368255
100	22.226926793065786	25.91002814574173	26.334489735906125	25.528555325286355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	102.0
2	88.5
3	111.0
4	75.0
5	61.5
6	152.5
7	378.0
8	621.0
9	689.0
10	545.0
11	390.5
12	404.0
13	515.5
14	698.0
15	959.0
16	1285.0
17	1744.0
18	2344.5
19	3088.5
20	4078.5
21	5091.0
22	6241.0
23	7603.0
24	8859.0
25	10303.0
26	12596.0
27	15250.5
28	19171.5
29	28441.5
30	41829.0
31	54594.0
32	72977.5
33	92708.0
34	108186.5
35	123882.5
36	139040.0
37	167092.0
38	180622.5
39	170555.5
40	159545.5
41	149954.0
42	149661.5
43	158340.5
44	174200.0
45	208689.0
46	241842.0
47	266189.0
48	282634.5
49	274694.0
50	260249.5
51	264521.0
52	266671.5
53	255418.5
54	244547.5
55	211665.0
56	183595.5
57	151790.0
58	117931.5
59	112871.5
60	93503.0
61	63232.0
62	45466.5
63	36864.0
64	26370.5
65	12102.0
66	6356.5
67	4342.0
68	3114.5
69	2267.0
70	1635.5
71	1256.5
72	922.0
73	674.0
74	489.0
75	324.5
76	218.0
77	127.0
78	63.0
79	32.0
80	18.5
81	11.5
82	8.0
83	3.0
84	1.5
85	1.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01357150864632771
2	0.0042848190958122276
3	0.0018538400985963106
4	0.02817487168715714
5	0.003252964701310507
6	0.0
7	5.246717260178239E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009444091068320828
16-17	0.0027982492054283935
18-19	0.0
20-21	5.246717260178238E-5
22-23	7.257958876579895E-4
24-25	0.0
26-27	1.8363510410623833E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.54715495882114E-4
36-37	0.0
38-39	0.0
40-41	3.148030356106943E-4
42-43	5.946279561535337E-4
44-45	0.0
46-47	0.0
48-49	4.372264383481865E-4
50-51	0.0
52-53	0.0
54-55	2.885694493098031E-4
56-57	7.870075890267357E-5
58-59	2.011241616401658E-4
60-61	6.995623013570984E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013204238438115233
70-71	6.908177725901347E-4
72-73	0.0
74-75	1.8363510410623833E-4
76-77	0.0027020593889917925
78-79	4.54715495882114E-4
80-81	0.0029643952520007045
82-83	0.001810117454761492
84-85	0.005561520295788932
86-87	0.007738907958762901
88-89	0.007467827566987025
90-91	0.005640221054691605
92-93	0.008447214788886964
94-95	0.001451591775315979
96-97	0.004503432314986321
98-99	0.0018888182136641658
100	0.08291562176835009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5717861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.09390114027945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.69227518418045	13.646124572748555
2	16.14382795065068	6.810726216334112
3	7.009295966968536	4.435601885705812
4	3.6518115951034145	3.0812381108015043
5	2.1327243071787936	2.2493737847550226
6	1.3526411292984728	1.7119486955818761
7	0.9207765688357491	1.35959389407149
8	0.6540911487720505	1.1037867223143558
9	0.4817785234410217	0.9146329690477243
>10	2.2882600398025468	8.743198020036827
>50	0.24508993325500972	3.663671049963055
>100	0.3023041287713994	14.288628401198208
>500	0.06397043730674738	9.380155959272875
>1k	0.0570927866582018	21.796885054364754
>5k	0.003480256951588499	4.88572148185036
>10k+	5.800428252647498E-4	1.9287131819535632
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	23381	0.408911654200758	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16633	0.29089549396181547	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16278	0.2846868785372712	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14703	0.2571416129213354	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	14481	0.2532590421488035	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	13668	0.23904043837372052	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11200	0.19587744437998758	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9597	0.16784248515310185	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8924	0.15607234943276865	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8869	0.15511045126840264	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8769	0.15336154551500988	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8427	0.1473802878384067	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8328	0.14564887114254788	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8246	0.14421476842476583	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	8206	0.14351520612340873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8061	0.14097929278098925	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7722	0.13505050227698787	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7435	0.13003114276475067	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7377	0.12901677742778286	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7356	0.12864950721957039	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	7271	0.12716293732918654	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7261	0.12698804675384728	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7002	0.12245838085256007	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6959	0.1217063513786012	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6863	0.12002740185534416	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6837	0.11957268635946203	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6801	0.11894308028824066	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6524	0.11409861135134275	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6372	0.11144027460618577	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6164	0.10780255063912886	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5997	0.10488187803096298	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5991	0.10477694368575942	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5952	0.10409487044193623	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5887	0.10295808170223096	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5831	0.10197869448033102	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5793	0.10131411029404176	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.748905753392746E-5
7	0.0	0.0	0.0	0.0	1.748905753392746E-5
8	0.0	0.0	0.0	0.0	1.748905753392746E-5
9	0.0	0.0	0.0	1.748905753392746E-5	1.748905753392746E-5
10-11	0.0	0.0	0.0	1.748905753392746E-5	1.748905753392746E-5
12-13	0.0	0.0	0.0	1.748905753392746E-5	1.748905753392746E-5
14-15	0.0	0.0	0.0	1.748905753392746E-5	1.748905753392746E-5
16-17	0.0	0.0	0.0	1.748905753392746E-5	1.748905753392746E-5
18-19	0.0	0.0	0.0	2.623358630089119E-5	1.748905753392746E-5
20-21	0.0	0.0	0.0	5.246717260178238E-5	1.748905753392746E-5
22-23	0.0	0.0	0.0	1.136788739705285E-4	1.748905753392746E-5
24-25	0.0	0.0	0.0	2.27357747941057E-4	1.748905753392746E-5
26-27	0.0	0.0	0.0	5.246717260178238E-4	3.497811506785492E-5
28-29	0.0	0.0	0.0	0.0016264823506552538	3.497811506785492E-5
30-31	0.0	0.0	0.0	0.006374761471116559	3.497811506785492E-5
32-33	0.0	0.0	0.0	0.018083685490080992	3.497811506785492E-5
34-35	0.0	0.0	0.0	0.03535412980483436	3.497811506785492E-5
36-37	0.0	0.0	0.0	0.06767390812753231	3.497811506785492E-5
38-39	0.0	0.0	0.0	0.11734283152388629	3.497811506785492E-5
40-41	0.0	0.0	0.0	0.17429594738312107	3.497811506785492E-5
42-43	0.0	0.0	0.0	0.23555137139570198	3.497811506785492E-5
44-45	0.0	0.0	0.0	0.30973995345462224	3.497811506785492E-5
46-47	0.0	0.0	0.0	0.40886793155692314	6.12117013687461E-5
48-49	0.0	0.0	0.0	0.5260883396780719	8.74452876696373E-5
50-51	0.0	0.0	0.0	0.6523768241305621	8.74452876696373E-5
52-53	0.0	0.0	0.0	0.7797321410926219	8.74452876696373E-5
54-55	0.0	0.0	0.0	0.9207813901037469	8.74452876696373E-5
56-57	0.0	0.0	0.0	1.0923490445115753	8.74452876696373E-5
58-59	0.0	0.0	0.0	1.2994719528858782	8.74452876696373E-5
60-61	0.0	0.0	0.0	1.5177966026106615	8.74452876696373E-5
62-63	0.0	0.0	0.0	1.7543186166994964	8.74452876696373E-5
64-65	0.0	0.0	0.0	2.0009143279278736	8.74452876696373E-5
66-67	0.0	0.0	0.0	2.2880759081061957	8.74452876696373E-5
68-69	0.0	0.0	0.0	2.6066128575003833	8.74452876696373E-5
70-71	0.0	0.0	0.0	2.957460840688502	8.74452876696373E-5
72-73	0.0	0.0	0.0	3.3310970658433283	1.0493434520356476E-4
74-75	0.0	0.0	0.0	3.699574019025646	1.2242340273749223E-4
76-77	0.0	0.0	0.0	4.132174601656109	1.5740151780534715E-4
78-79	0.0	0.0	0.0	4.597191152425706	1.5740151780534715E-4
80-81	0.0	0.0	0.0	5.098803556085047	1.5740151780534715E-4
82-83	0.0	0.0	0.0	5.623615194563142	1.748905753392746E-4
84-85	0.0	0.0	0.0	6.149178862515196	1.748905753392746E-4
86-87	0.0	0.0	0.0	6.715946750017183	1.748905753392746E-4
88	0.0	0.0	0.0	7.166386171332252	1.748905753392746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11455	0.0	39.5674	1
GGGCACC	5750	0.0	37.95767	94
GTATCAA	18840	0.0	36.66034	1
TACCCCG	390	0.0	32.536903	5
CAACGCA	20290	0.0	32.381886	5
ATCAACG	20740	0.0	31.733494	3
GTACTAG	6025	0.0	30.668398	1
TATCAAC	23750	0.0	28.979523	2
TAGTACT	8160	0.0	28.689976	4
AACGCAG	24145	0.0	28.242184	6
CTGTGCG	8260	0.0	27.65678	9
GTGGTAT	2700	0.0	26.991282	1
TAGGCAT	7965	0.0	26.965437	5
ACGCAGA	24720	0.0	26.444584	7
CGCAGAG	24855	0.0	26.225317	8
CTAACGC	2880	0.0	26.117168	3
ACTGTGC	9390	0.0	25.524817	8
TCAACGC	26425	0.0	25.155064	4
TAACGCC	3015	0.0	25.103228	4
TACTGTG	10060	0.0	25.08617	7
>>END_MODULE