##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146465_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3825204 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.59016251159415 31.0 31.0 34.0 27.0 34.0 2 30.70217823676855 31.0 31.0 34.0 27.0 34.0 3 30.87936826375796 31.0 31.0 34.0 27.0 34.0 4 34.350344190793486 37.0 35.0 37.0 30.0 37.0 5 34.58595619998306 37.0 35.0 37.0 31.0 37.0 6 34.69767050332479 37.0 35.0 37.0 31.0 37.0 7 34.60035386348022 37.0 35.0 37.0 30.0 37.0 8 34.53656955289182 37.0 35.0 37.0 30.0 37.0 9 35.92026778179674 39.0 35.0 39.0 30.0 39.0 10-11 35.838176343013345 39.0 35.0 39.0 30.0 39.0 12-13 35.826032546238054 39.0 35.0 39.0 30.0 39.0 14-15 36.847280040489345 40.0 36.0 41.0 30.0 41.0 16-17 36.689744259391134 40.0 36.0 41.0 29.5 41.0 18-19 36.63327249997647 40.0 36.0 41.0 28.5 41.0 20-21 36.97680489720287 40.0 36.0 41.0 30.0 41.0 22-23 36.6952918066592 40.0 36.0 41.0 29.0 41.0 24-25 36.80705578055445 40.0 36.0 41.0 30.0 41.0 26-27 36.881014711895105 40.0 36.0 41.0 30.0 41.0 28-29 36.71566745198426 40.0 36.0 41.0 29.5 41.0 30-31 36.65772099997804 40.0 35.0 41.0 29.5 41.0 32-33 36.75700838438944 40.0 35.5 41.0 30.0 41.0 34-35 36.80534280001798 40.0 35.0 41.0 30.0 41.0 36-37 36.730798148281764 40.0 35.0 41.0 30.0 41.0 38-39 36.55635424934199 39.0 35.0 41.0 29.5 41.0 40-41 36.509042001420056 39.0 35.0 41.0 29.0 41.0 42-43 36.34749414671741 39.0 35.0 41.0 29.0 41.0 44-45 36.147475794754996 39.0 35.0 41.0 28.0 41.0 46-47 36.01462510234748 39.0 35.0 41.0 28.0 41.0 48-49 35.88324544259601 38.0 34.0 40.0 28.0 41.0 50-51 35.64692562801879 38.0 34.0 40.0 27.5 41.0 52-53 35.44829765941895 38.0 34.0 40.0 27.0 41.0 54-55 35.24804990792648 37.5 34.0 40.0 27.0 41.0 56-57 35.08709927627389 37.0 33.0 40.0 27.0 41.0 58-59 34.81157436309279 37.0 33.0 40.0 26.5 41.0 60-61 34.54395491064006 36.0 33.0 39.5 26.0 41.0 62-63 34.26775722288275 35.5 33.0 39.0 26.0 41.0 64-65 33.97836612112714 35.0 33.0 39.0 26.0 41.0 66-67 33.68573323148256 35.0 32.0 38.5 26.0 40.5 68-69 33.39954601114084 35.0 32.0 38.0 25.0 40.0 70-71 33.33939248730264 35.0 32.0 37.0 26.0 40.0 72-73 33.13698328246023 35.0 32.0 37.0 25.0 40.0 74-75 32.86612661703794 35.0 32.0 36.0 25.0 39.0 76-77 32.644525886723954 35.0 32.0 36.0 25.0 39.0 78-79 32.42346264408382 35.0 32.0 36.0 25.0 39.0 80-81 32.20623056966373 35.0 31.5 35.0 24.0 37.5 82-83 31.972210632426403 35.0 31.0 35.0 24.0 37.0 84-85 31.78575181872653 35.0 31.0 35.0 24.0 37.0 86-87 31.616548424606897 34.5 31.0 35.0 24.0 36.0 88-89 31.622700645508058 35.0 31.0 35.0 24.0 36.0 90-91 31.536948748354334 35.0 31.0 35.0 24.0 36.0 92-93 31.38777801131652 35.0 31.0 35.0 23.0 36.0 94-95 31.358822170007144 35.0 31.0 35.0 23.0 35.0 96-97 31.254767588865846 35.0 31.0 35.0 23.0 35.0 98-99 31.16239486835212 35.0 31.0 35.0 22.5 35.0 100 30.657632638677573 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7526069660105321 1101 2 0.9791021049748352 1101 3 0.9144157938952944 1101 4 0.9389688301986396 1101 5 0.8367007467060716 1101 6 0.6032375495711619 1101 7 0.6618251467500471 1101 8 0.6913417288431347 1101 9 0.9094233428787959 1101 10-11 0.9046656877730896 1101 12-13 0.7280519027514671 1101 14-15 1.0040716839720503 1101 16-17 1.1379459418474056 1101 18-19 1.2563875004497547 1101 20-21 0.9041310835450815 1101 22-23 1.2432024783089233 1101 24-25 1.1031100744676579 1101 26-27 1.0022862762974114 1101 28-29 1.2016237628934476 1101 30-31 1.2346303332171402 1101 32-33 1.019221553224746 1101 34-35 0.977976261135332 1101 36-37 0.9415672513921578 1101 38-39 1.0421101670670865 1101 40-41 0.9416545750179921 1101 42-43 0.9829953037569936 1101 44-45 1.0346803825330824 1101 46-47 0.9730150224787977 1101 48-49 0.9672605593752408 1101 50-51 0.9183260426461644 1101 52-53 0.9930297108059065 1101 54-55 1.0052934329943781 1101 56-57 0.9429032688239047 1101 58-59 0.895883676824262 1101 60-61 0.8909802415868668 1101 62-63 0.798847105007539 1101 64-65 0.7637852209030243 1101 66-67 0.7552259605945508 1101 68-69 0.8985311871635489 1101 70-71 0.7560082349395927 1101 72-73 0.7412977925619728 1101 74-75 0.7397301345771794 1101 76-77 0.7736309585430377 1101 78-79 0.7217091577279291 1101 80-81 0.7980312810335732 1101 82-83 0.8000959478514176 1101 84-85 0.8167914973659798 1101 86-87 0.7148254574107362 1101 88-89 0.6913320402295113 1101 90-91 0.7787040061547827 1101 92-93 0.8289162646756267 1101 94-95 0.7773950595308463 1101 96-97 0.8017230795034322 1101 98-99 0.8215599350064018 1101 100 0.8926896212415478 1102 1 0.7708034897846083 1102 2 0.8902675953475132 1102 3 0.8507795860526066 1102 4 0.9311824563924489 1102 5 0.7396188331973761 1102 6 0.5996413381229289 1102 7 0.6478363190526508 1102 8 0.6793574473493109 1102 9 0.856053081602596 1102 10-11 0.8588835015678029 1102 12-13 0.8092097081714655 1102 14-15 1.06177647872142 1102 16-17 1.205186152208114 1102 18-19 1.215108872766045 1102 20-21 0.9829380441935598 1102 22-23 1.249466483176164 1102 24-25 1.0632739839392755 1102 26-27 0.9459838283707285 1102 28-29 1.1661079044224536 1102 30-31 1.1773758196110293 1102 32-33 1.0716090114502705 1102 34-35 0.920938936940594 1102 36-37 0.9111867446914701 1102 38-39 0.9649272251859671 1102 40-41 0.9183096330414884 1102 42-43 0.9686410123747677 1102 44-45 0.9780758819964603 1102 46-47 0.9101201860034465 1102 48-49 0.8647047256112401 1102 50-51 0.9912019561224099 1102 52-53 0.9053193107553668 1102 54-55 0.96159926874045 1102 56-57 0.845571949601549 1102 58-59 0.8080041145049179 1102 60-61 0.9093179346666531 1102 62-63 0.8885389982332299 1102 64-65 0.8125019569991565 1102 66-67 0.7875719666695744 1102 68-69 0.8575901979153997 1102 70-71 0.8695660807816736 1102 72-73 0.8345298960629961 1102 74-75 0.7411028112984681 1102 76-77 0.7845880385610826 1102 78-79 0.8146836070441381 1102 80-81 0.8591964085345083 1102 82-83 0.8120163761157002 1102 84-85 0.7712271256872505 1102 86-87 0.7618805306077583 1102 88-89 0.7005975737930754 1102 90-91 0.7032262717820288 1102 92-93 0.7901962334601222 1102 94-95 0.7864329139452337 1102 96-97 0.746247354037429 1102 98-99 0.7881286527298776 1102 100 0.780449335257142 1103 1 0.632126041372647 1103 2 0.7397784624410519 1103 3 0.6628412112255475 1103 4 0.6047736705053168 1103 5 0.650732767133853 1103 6 0.5189981030782036 1103 7 0.5500963225003588 1103 8 0.5868883232080861 1103 9 0.6686153662189156 1103 10-11 0.7483141859449276 1103 12-13 0.6503559797439138 1103 14-15 0.8388185274798161 1103 16-17 1.0572183059985392 1103 18-19 1.1026816645656652 1103 20-21 0.8079064653427039 1103 22-23 0.9990355951695875 1103 24-25 0.9474264070996128 1103 26-27 0.8344862676416795 1103 28-29 1.0438234262343897 1103 30-31 1.0417097468233933 1103 32-33 0.8514488993479219 1103 34-35 0.7996044480583677 1103 36-37 0.7274252466542421 1103 38-39 0.8183316913058505 1103 40-41 0.7535010549818608 1103 42-43 0.8023927029033615 1103 44-45 0.7915378668021802 1103 46-47 0.7919313077423666 1103 48-49 0.7753628003936726 1103 50-51 0.8236868585304649 1103 52-53 0.7533886067752888 1103 54-55 0.7962227629470888 1103 56-57 0.7304691040608731 1103 58-59 0.7419244718649551 1103 60-61 0.7781944242348047 1103 62-63 0.7094799472598794 1103 64-65 0.7135514478832974 1103 66-67 0.8315869767418747 1103 68-69 0.8840546984134434 1103 70-71 0.7593548055727339 1103 72-73 0.7087715633298117 1103 74-75 0.7062562837295374 1103 76-77 0.7337298936181611 1103 78-79 0.7511737156348488 1103 80-81 0.7397260700958412 1103 82-83 0.6881013392989388 1103 84-85 0.6227066420479908 1103 86-87 0.7029215403492763 1103 88-89 0.7136041488047766 1103 90-91 0.7216388891117376 1103 92-93 0.7925639385525542 1103 94-95 0.7510303712068733 1103 96-97 0.7233518125694616 1103 98-99 0.655949674020853 1103 100 0.7470137227577212 1104 1 0.5073007028994567 1104 2 0.676087800967391 1104 3 0.6587946264579969 1104 4 0.7656405607829271 1104 5 0.6832813720586373 1104 6 0.3106414912453346 1104 7 0.44500323910727246 1104 8 0.4606587354700551 1104 9 0.6531597563058043 1104 10-11 0.5843591416280631 1104 12-13 0.5358294794283793 1104 14-15 0.7313148261349411 1104 16-17 0.8399045216781502 1104 18-19 0.8757364710439575 1104 20-21 0.7417525900193169 1104 22-23 1.0207709283712418 1104 24-25 0.7882335384046826 1104 26-27 0.6751590950302457 1104 28-29 0.8749465225816238 1104 30-31 0.8963299075491733 1104 32-33 0.7569628245709694 1104 34-35 0.6270442341156226 1104 36-37 0.6311512397918406 1104 38-39 0.7600659615418763 1104 40-41 0.65816513022191 1104 42-43 0.6706254672962757 1104 44-45 0.6243131282476853 1104 46-47 0.6453176221493919 1104 48-49 0.537885379082617 1104 50-51 0.6374435895886634 1104 52-53 0.6553273396456021 1104 54-55 0.660104504437129 1104 56-57 0.5932819380571459 1104 58-59 0.5245346675864866 1104 60-61 0.5585796925462958 1104 62-63 0.446115087739301 1104 64-65 0.4852024828341115 1104 66-67 0.543074007441426 1104 68-69 0.5716932590445083 1104 70-71 0.4875194535750964 1104 72-73 0.41122166926061254 1104 74-75 0.41438540300129745 1104 76-77 0.44996803733380375 1104 78-79 0.4277043450941349 1104 80-81 0.446807615832995 1104 82-83 0.3801217816807565 1104 84-85 0.40481449693108473 1104 86-87 0.32399053443295145 1104 88-89 0.33600485072039277 1104 90-91 0.42444813410605775 1104 92-93 0.3719789969238505 1104 94-95 0.33953957832558146 1104 96-97 0.3448620120382486 1104 98-99 0.4485303381086361 1104 100 0.481598011379166 1105 1 0.5599335280036648 1105 2 0.6085330881616215 1105 3 0.6883595525333632 1105 4 0.7671612198897719 1105 5 0.6480120616267726 1105 6 0.3827751928614873 1105 7 0.482996010818006 1105 8 0.4758293328921326 1105 9 0.5606575632146686 1105 10-11 0.6104234102832535 1105 12-13 0.5627304989295752 1105 14-15 0.7201186285941574 1105 16-17 0.900785557460523 1105 18-19 0.8711636985275746 1105 20-21 0.6950779740312356 1105 22-23 0.9632989719716605 1105 24-25 0.8241190299222652 1105 26-27 0.6423038657069569 1105 28-29 0.8736698783718637 1105 30-31 0.9048613870648126 1105 32-33 0.7495397146213989 1105 34-35 0.6650937059624837 1105 36-37 0.6129445261363671 1105 38-39 0.7246445620150084 1105 40-41 0.5968963078198328 1105 42-43 0.6055090052456649 1105 44-45 0.6344301539669104 1105 46-47 0.5498614867251845 1105 48-49 0.5851245041182267 1105 50-51 0.6589981760399226 1105 52-53 0.6448027481894272 1105 54-55 0.6796207260664104 1105 56-57 0.6481573383125792 1105 58-59 0.6818161648526271 1105 60-61 0.6509050183076326 1105 62-63 0.6153810636873089 1105 64-65 0.6087678013039834 1105 66-67 0.6995259120182737 1105 68-69 0.615872359023669 1105 70-71 0.5937930997633458 1105 72-73 0.6244036322126547 1105 74-75 0.6476177831405678 1105 76-77 0.6234839877066207 1105 78-79 0.6217004483906834 1105 80-81 0.7144266361276834 1105 82-83 0.6872700126162172 1105 84-85 0.593792630935841 1105 86-87 0.6196533580098951 1105 88-89 0.6368536718994946 1105 90-91 0.6623488785833302 1105 92-93 0.7032054412993176 1105 94-95 0.6465698735575245 1105 96-97 0.6825751084800658 1105 98-99 0.6942115497440291 1105 100 0.7381531875813963 1106 1 0.47071887779628696 1106 2 0.5253916855471417 1106 3 0.554749483085871 1106 4 0.6071901585936885 1106 5 0.53431589126955 1106 6 0.2981029651834106 1106 7 0.34091814460429504 1106 8 0.34818494373521247 1106 9 0.5998301135085526 1106 10-11 0.4716171628156616 1106 12-13 0.38073138759391867 1106 14-15 0.5506563887639615 1106 16-17 0.6805790885099086 1106 18-19 0.6285120835916516 1106 20-21 0.47711635546822606 1106 22-23 0.7804785119590107 1106 24-25 0.586745689129252 1106 26-27 0.47587932592022497 1106 28-29 0.6912742489335244 1106 30-31 0.6705928323796115 1106 32-33 0.5399060955114763 1106 34-35 0.5185061207789659 1106 36-37 0.39068753396036726 1106 38-39 0.46840547193107085 1106 40-41 0.43850539240085595 1106 42-43 0.4314139524863094 1106 44-45 0.42601348323920973 1106 46-47 0.4415309100192175 1106 48-49 0.43844691598378205 1106 50-51 0.5325933508026424 1106 52-53 0.43309840111003695 1106 54-55 0.43323154917381856 1106 56-57 0.3929240225952526 1106 58-59 0.4851888905489403 1106 60-61 0.4724286213232105 1106 62-63 0.4566383589918672 1106 64-65 0.4984318176477842 1106 66-67 0.5049607270079619 1106 68-69 0.46883465863579943 1106 70-71 0.4202657170830193 1106 72-73 0.36912501367070405 1106 74-75 0.44158689376045146 1106 76-77 0.3852918094796536 1106 78-79 0.3741386644931666 1106 80-81 0.48192938314057443 1106 82-83 0.4151735993658363 1106 84-85 0.4366302730169487 1106 86-87 0.4406141268830588 1106 88-89 0.39526292082774006 1106 90-91 0.3913068717149848 1106 92-93 0.4603058303497036 1106 94-95 0.42727237414142394 1106 96-97 0.4484975087164145 1106 98-99 0.4696656671523023 1106 100 0.3965187466205471 1107 1 0.45730865993540704 1107 2 0.6049181842824574 1107 3 0.4887226300700682 1107 4 0.5849061643895936 1107 5 0.47823734359582915 1107 6 0.22355915465398368 1107 7 0.22199008281728538 1107 8 0.23450439488581765 1107 9 0.29457707199902217 1107 10-11 0.3096524182566043 1107 12-13 0.2697261849901693 1107 14-15 0.5084775132978052 1107 16-17 0.587446417232826 1107 18-19 0.6848781476886145 1107 20-21 0.4307668949391257 1107 22-23 0.6541036702081016 1107 24-25 0.4026290698459576 1107 26-27 0.3971790864845275 1107 28-29 0.567278826992748 1107 30-31 0.5537314578896115 1107 32-33 0.41612685133252825 1107 34-35 0.23830318648523274 1107 36-37 0.31515107698947986 1107 38-39 0.35633090070082574 1107 40-41 0.14757508995226942 1107 42-43 0.2900499862899295 1107 44-45 0.24440541859809883 1107 46-47 0.262043353964323 1107 48-49 0.2342375639720231 1107 50-51 0.2951025676461114 1107 52-53 0.24721378246183434 1107 54-55 0.22214397865872826 1107 56-57 0.07524070399269078 1107 58-59 0.15822578587254554 1107 60-61 0.24993318295292966 1107 62-63 0.2794991489372123 1107 64-65 0.3075411078076087 1107 66-67 0.2876269022955995 1107 68-69 0.28283592602326024 1107 70-71 0.2675004102051659 1107 72-73 0.23762211462929628 1107 74-75 0.13093986841945338 1107 76-77 0.11968431900668008 1107 78-79 0.15631719853792703 1107 80-81 0.2594921732964579 1107 82-83 0.2877990679625171 1107 84-85 0.19109166598387972 1107 86-87 0.3445622608449881 1107 88-89 0.4188230751212032 1107 90-91 0.3189176561233893 1107 92-93 0.40023072768479295 1107 94-95 0.3324833070123461 1107 96-97 0.31828116024388464 1107 98-99 0.3202697208172758 1107 100 0.3726033216092084 1108 1 0.3596191121493 1108 2 0.45112771660256 1108 3 0.39786389126001254 1108 4 0.5071185398572595 1108 5 0.4087554026113196 1108 6 0.2563207437786943 1108 7 0.20266053987212018 1108 8 0.23859084372443817 1108 9 0.2476071753756841 1108 10-11 0.3912020943473635 1108 12-13 0.19591537801344572 1108 14-15 0.3446856346823566 1108 16-17 0.6019479065019055 1108 18-19 0.6525267745050414 1108 20-21 0.26553386601801776 1108 22-23 0.45896908927432634 1108 24-25 0.3381600079667564 1108 26-27 0.34239523627335444 1108 28-29 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-2.1167378069157046 2215 96-97 -1.6135086816435873 2215 98-99 -3.0884931675127056 2215 100 -3.0522314843161027 2216 1 -0.6425157723840513 2216 2 -0.5997807732666907 2216 3 -0.7857586142888486 2216 4 -0.7592577533807017 2216 5 -0.7978846447802681 2216 6 -0.7062408996588587 2216 7 -0.8548924796368809 2216 8 -0.7198092986908122 2216 9 -0.8393130501630282 2216 10-11 -0.9267195272625628 2216 12-13 -1.0559427081443076 2216 14-15 -1.469737865964703 2216 16-17 -1.1695055592449606 2216 18-19 -3.139806269144067 2216 20-21 -1.4099895421563957 2216 22-23 -0.9020095360475295 2216 24-25 -0.9583583776701872 2216 26-27 -0.9927744501797022 2216 28-29 -0.9424104629004688 2216 30-31 -1.0710052914733694 2216 32-33 -1.0297385723861012 2216 34-35 -1.3151604752494848 2216 36-37 -1.4063780237982044 2216 38-39 -1.4106289342503047 2216 40-41 -1.379252972838401 2216 42-43 -1.3586122124953661 2216 44-45 -1.251402972153052 2216 46-47 -1.3776168350664832 2216 48-49 -1.3609639995921583 2216 50-51 -1.2357115630188673 2216 52-53 -1.2653401959687613 2216 54-55 -1.179484913381721 2216 56-57 -1.2427826553118564 2216 58-59 -1.3472384163003568 2216 60-61 -1.3937975547825587 2216 62-63 -1.2812902823876016 2216 64-65 -1.380571359530336 2216 66-67 -1.4062665049414562 2216 68-69 -1.4993967054129946 2216 70-71 -1.4919868505638831 2216 72-73 -1.4159775235043028 2216 74-75 -1.502702901643083 2216 76-77 -1.394698196742027 2216 78-79 -1.4240183543881741 2216 80-81 -1.4328953752828184 2216 82-83 -1.534640236241259 2216 84-85 -1.3820191879607577 2216 86-87 -1.4903802436166913 2216 88-89 -1.381896061875544 2216 90-91 -1.3464752966882614 2216 92-93 -1.3242455621592555 2216 94-95 -1.2384427030249228 2216 96-97 -1.2394285132558238 2216 98-99 -1.214564516658971 2216 100 -1.122959884175632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15697.0 1 2596.0 2 430.0 3 401.0 4 393.0 5 218.0 6 202.0 7 297.0 8 538.0 9 905.0 10 1508.0 11 2443.0 12 3823.0 13 5345.0 14 7321.0 15 9940.0 16 12855.0 17 15200.0 18 17418.0 19 19662.0 20 22175.0 21 25372.0 22 29646.0 23 33874.0 24 38098.0 25 43265.0 26 49164.0 27 56692.0 28 67934.0 29 80915.0 30 96410.0 31 116676.0 32 145093.0 33 184734.0 34 256932.0 35 349155.0 36 512023.0 37 782216.0 38 615501.0 39 202040.0 40 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.314978086400686 21.403927918279358 11.998296342417218 24.28279765290274 2 15.495413639073222 20.71207422210434 40.742431125805204 23.05008101301723 3 18.22107362466008 28.552383544255918 29.04558407552023 24.180958755563775 4 12.075617395415417 17.66964995108114 40.90107884144379 29.353653812059648 5 12.912845969826177 39.004624615304635 35.16603374580186 12.916495669067329 6 28.180134759298163 39.239470829779236 19.282154152258723 13.298240258663876 7 25.679290207616585 32.09846246332724 25.19906945899035 17.02317787006583 8 25.448130478547988 34.770710589728964 21.1085148272391 18.672644104483947 9 26.68862898279403 14.936013030226741 22.105502159353282 36.26985582762595 10-11 25.422266131346806 25.852272428609126 27.81644794455886 20.90901349548521 12-13 25.954844593493586 24.03749415221585 27.54195949391042 22.465701760380146 14-15 23.36077524348897 24.969707195620227 25.2345860841933 26.434931476697503 16-17 22.230439968313494 27.211997348594164 26.688536559595917 23.86902612349643 18-19 21.68164124792222 28.073295659353565 28.125071865768742 22.11999122695548 20-21 23.45592768914659 27.19444317415463 26.756061779187185 22.59356735751159 22-23 24.05522233389209 26.8034264244947 26.40191406038081 22.739437181232404 24-25 23.473892455484354 26.92779795964896 27.107509692882953 22.490799891983734 26-27 23.12722271657893 27.43067922684509 26.261350807328366 23.180747249247617 28-29 22.3699164506436 27.93805601577702 26.728975032419882 22.963052501159495 30-31 23.170193803659437 27.455354180684488 26.633132948277638 22.74131906737844 32-33 23.618239079902175 27.7312152190563 25.608655428320425 23.041890272721098 34-35 23.52262553354607 27.322723277228178 26.734473991678254 22.420177197547495 36-37 23.3008245062229 27.182115588595973 26.387958239739017 23.129101665442107 38-39 23.59520600648828 26.941828243926192 26.97129294443959 22.49167280514594 40-41 22.939039977394224 26.26494100483987 27.663239657364784 23.13277936040112 42-43 22.96738351028373 26.555557483119795 26.887089770521726 23.58996923607475 44-45 22.945312566746694 27.63493429003865 26.706181803364988 22.713571339849672 46-47 22.87377433101297 27.1618052614686 26.38779468827046 23.576625719247968 48-49 23.70866402355386 26.33319278136662 26.903525419123202 23.05461777595631 50-51 23.3760166343838 26.556992963477978 26.401577858880298 23.665412543257926 52-53 22.62916420932455 27.183525873315734 26.463419505882378 23.72389041147734 54-55 22.76587554574622 27.121489242387742 26.319026604672285 23.79360860719375 56-57 23.792346692257325 26.423071853882007 26.198947736216322 23.585633717644345 58-59 22.664397603027755 26.647865853658537 27.26964623633305 23.41809030698066 60-61 22.80482652816515 27.409831110935905 26.318967414693116 23.46637494620583 62-63 23.320996448201704 26.613981636712698 25.891467734558166 24.173554180527432 64-65 22.212265550608322 26.738525538271336 26.596153456971276 24.453055454149066 66-67 23.240513062574838 26.231840597795063 26.421214029429297 24.106432310200805 68-69 22.35272159943009 26.534059916852982 26.687998885414572 24.425219598302363 70-71 22.41886913206119 26.160603932076697 26.934403404181502 24.48612353168061 72-73 23.05911553141264 26.40724949777465 27.209075303444518 23.32455966736819 74-75 23.177834155675274 26.76566310066609 26.759092250141762 23.29741049351687 76-77 22.694109136970795 26.63475834538382 26.604483765190672 24.066648752454718 78-79 22.631915504676332 26.673321946189503 26.54527244037358 24.149490108760588 80-81 22.25029050541701 26.874408755937733 27.37602419338837 23.499276545256887 82-83 23.091727336231596 25.73234485437465 28.221978335038965 22.95394947435479 84-85 23.420230096292237 26.319234687494415 26.785504017696155 23.47503119851719 86-87 22.038207106249157 27.04165359978307 27.325361512718676 23.5947777812491 88-89 23.080149223034613 25.63420054065857 27.234525591401486 24.05112464490533 90-91 22.756540134242947 25.693570501337383 27.38995189447076 24.159937469948915 92-93 22.98305980776665 26.00625799182562 27.365940532557087 23.644741667850642 94-95 21.912827249384982 26.03361301666112 27.826475534085983 24.22708419986791 96-97 22.285198629432728 25.835267075856187 27.46517479279065 24.414359501920437 98-99 21.780868824223077 26.6196243300174 27.315281583326957 24.284225262432564 100 22.54511729889824 25.714838478457715 26.581847571956946 25.1581966506871 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13364.0 1 7864.0 2 2221.0 3 1680.5 4 984.5 5 609.0 6 492.0 7 455.5 8 463.0 9 468.5 10 452.5 11 411.0 12 383.0 13 386.0 14 396.0 15 439.0 16 506.0 17 577.5 18 639.0 19 745.5 20 896.0 21 1076.0 22 1385.0 23 1882.5 24 2577.5 25 3433.0 26 4676.0 27 6302.0 28 8973.0 29 14910.5 30 23849.0 31 33310.0 32 45620.0 33 59138.0 34 73766.0 35 87393.0 36 101373.5 37 125869.0 38 134314.5 39 122505.5 40 112471.0 41 104025.5 42 101777.5 43 108463.5 44 119138.0 45 140013.5 46 162492.0 47 178404.5 48 187284.5 49 181587.0 50 171366.5 51 171626.5 52 171240.5 53 164781.5 54 156880.0 55 137593.5 56 121307.5 57 99889.5 58 77914.5 59 72249.5 60 59437.0 61 41781.0 62 31237.5 63 25217.5 64 18126.0 65 9479.5 66 5367.0 67 3577.5 68 2543.0 69 1904.5 70 1476.0 71 1123.5 72 825.5 73 658.5 74 521.0 75 379.0 76 257.0 77 169.5 78 115.0 79 85.5 80 62.0 81 48.0 82 42.0 83 25.5 84 19.0 85 18.0 86 18.0 87 15.0 88 9.5 89 8.5 90 7.0 91 5.5 92 8.0 93 6.0 94 2.0 95 2.0 96 2.5 97 1.5 98 0.0 99 1.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9949273293659633 2 0.44896951901127363 3 0.48196122350598825 4 0.4673999086061815 5 0.4358198935272472 6 0.48533359266590753 7 0.5031365647426909 8 0.5055155228322463 9 0.5201814073184071 10-11 0.5326774728877205 12-13 0.5318409161968878 14-15 0.5295795988919807 16-17 0.5340891623035007 18-19 0.532455262516718 20-21 0.5306383659538159 22-23 0.4926665349089879 24-25 0.5286384726147939 26-27 0.509057817569991 28-29 0.5141947984996356 30-31 0.5171096757192558 32-33 0.5194363490156342 34-35 0.5321154113610673 36-37 0.551839849587107 38-39 0.5398797031478583 40-41 0.5455003184143904 42-43 0.5424024444186506 44-45 0.5536044613568323 46-47 0.5504804449645039 48-49 0.5220244462778979 50-51 0.5373438906787716 52-53 0.5301678028152225 54-55 0.5402456967000976 56-57 0.5272790679921907 58-59 0.533409459992199 60-61 0.5278542007171382 62-63 0.5215015983461274 64-65 0.5329127544570172 66-67 0.5303769419879306 68-69 0.5515784256212217 70-71 0.5351740717619243 72-73 0.5395006383973248 74-75 0.5364811915913504 76-77 0.5236714172629747 78-79 0.5543887332544879 80-81 0.5280110550966693 82-83 0.5271875696041309 84-85 0.536860256341884 86-87 0.8422688044872901 88-89 1.1086990393192102 90-91 1.099627627702993 92-93 1.09906556617634 94-95 1.1025686473192018 96-97 1.0967519640782557 98-99 1.0928436757882716 100 1.088098830807455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3825204.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.49253188650826 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25209500620528 20.688706214846476 2 12.321199948043258 6.774819649032464 3 4.676890818658443 3.8573870988505483 4 2.298695345109917 2.5278782029121 5 1.2748183309712773 1.7523991806866541 6 0.8063228745531935 1.3300714403684804 7 0.5393370064865292 1.0379417893883371 8 0.38112701895370593 0.8382517377115678 9 0.2774410613879931 0.6864801504152487 >10 1.5884821472601736 8.293635635993805 >50 0.21374205263128865 4.193401673324406 >100 0.2777655015780137 17.09341114637851 >500 0.05517363133610612 10.735152002350846 >1k 0.035482352566150704 17.007615133363426 >5k 0.0010463964563744176 1.968495646697121 >10k+ 3.8050780231797E-4 1.214353297680153 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 12217 0.31938165912197103 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11742 0.3069640207424232 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11541 0.30170939902813027 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 10933 0.285814821902309 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9500 0.24835276759095723 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 9447 0.2469672205717656 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8954 0.23407901905362433 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7148 0.18686585081475393 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6505 0.1700562898083344 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6320 0.16521994643945787 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5838 0.15261931128379036 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5629 0.1471555503967893 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5393 0.14098594480189816 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5349 0.1398356793520032 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5186 0.13557446870807413 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 4877 0.1274964681622209 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4632 0.12109158099803305 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 4582 0.11978446116860696 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 4559 0.11918318604707097 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4458 0.11654280399163025 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4424 0.1156539625076205 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 4263 0.1114450366568685 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4240 0.1108437615353325 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 4175 0.10914450575707857 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 3964 0.10362846007690048 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 3964 0.10362846007690048 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 3928 0.1026873337997137 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 3922 0.10253047942018255 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 3879 0.10140635636687612 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3855 0.10077893884875161 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 3838 0.10033451810674673 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.535599147130454E-5 14-15 0.0 0.0 0.0 0.0 7.842718976556544E-5 16-17 0.0 0.0 0.0 0.0 7.842718976556544E-5 18-19 0.0 0.0 0.0 1.3071198294260907E-5 1.0456958635408725E-4 20-21 0.0 0.0 0.0 5.228479317704363E-5 1.1764078464834816E-4 22-23 0.0 0.0 0.0 1.4378318123686997E-4 1.3071198294260908E-4 24-25 0.0 0.0 0.0 4.4442074200487085E-4 1.3071198294260908E-4 26-27 0.0 0.0 0.0 0.0010979806567179163 1.3071198294260908E-4 28-29 0.0 0.0 0.0 0.0034769387462734013 1.568543795311309E-4 30-31 0.0 0.0 0.0 0.012587563957373253 1.568543795311309E-4 32-33 0.0 0.0 0.0 0.03525302179962167 1.568543795311309E-4 34-35 0.0 0.0 0.0 0.07041454521118351 1.829967761196527E-4 36-37 0.0 0.0 0.0 0.1264899858935628 1.829967761196527E-4 38-39 0.0 0.0 0.0 0.21745245482332445 1.829967761196527E-4 40-41 0.0 0.0 0.0 0.32374743935225414 1.829967761196527E-4 42-43 0.0 0.0 0.0 0.43714008455496756 1.829967761196527E-4 44-45 0.0 0.0 0.0 0.5685840546020552 1.829967761196527E-4 46-47 0.0 0.0 0.0 0.7271507611097343 2.0913917270817453E-4 48-49 0.0 0.0 0.0 0.9058209705939866 2.6142396588521816E-4 50-51 0.0 0.0 0.0 1.10958788080322 2.6142396588521816E-4 52-53 0.0 0.0 0.0 1.3185440567352749 2.6142396588521816E-4 54-55 0.0 0.0 0.0 1.536937637835786 2.6142396588521816E-4 56-57 0.0 0.0 0.0 1.799551082765782 2.6142396588521816E-4 58-59 0.0 0.0 0.0 2.1140571849239933 2.6142396588521816E-4 60-61 0.0 0.0 0.0 2.4544181173082533 2.6142396588521816E-4 62-63 0.0 0.0 0.0 2.813026442511301 2.6142396588521816E-4 64-65 0.0 0.0 0.0 3.1829674966354737 2.6142396588521816E-4 66-67 0.0 0.0 0.0 3.5915077993225983 2.6142396588521816E-4 68-69 0.0 0.0 0.0 4.0372095187603065 2.6142396588521816E-4 70-71 0.0 0.0 0.0 4.540306869908115 2.744951641794791E-4 72-73 0.0 0.0 0.0 5.079519941942966 3.137087590622618E-4 74-75 0.0 0.0 0.0 5.609400178395715 3.398511556507836E-4 76-77 0.0 0.0 0.0 6.199760849356009 3.398511556507836E-4 78-79 0.0 0.0 0.0 6.825910461245988 3.398511556507836E-4 80-81 0.0 0.0 0.0 7.516252727959084 3.529223539450445E-4 82-83 0.0 0.0 0.0 8.251429204821495 3.659935522393054E-4 84-85 0.0 0.0 0.0 8.970776460549555 3.659935522393054E-4 86-87 0.0 2.6142396588521813E-5 0.0 9.718226792610277 3.9213594882782723E-4 88 0.0 2.6142396588521813E-5 0.0 10.293882365489527 4.18278345416349E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9085 0.0 35.60328 1 CAACGCA 8965 0.0 33.485043 5 GGGCACC 3710 0.0 32.813023 94 GTACTAG 3815 0.0 32.50514 1 GGTATCA 5720 0.0 31.736242 1 ATCAACG 9725 0.0 30.872684 3 TATCAAC 10890 0.0 29.772726 2 TAGGCAT 4670 0.0 28.871233 5 AACGCAG 11190 0.0 28.82467 6 AGGGCTA 4985 0.0 27.989271 5 ACGCAGA 10565 0.0 27.599407 7 CGCAGAG 10710 0.0 26.83152 8 GTACATG 18705 0.0 26.694946 1 TATTCTC 2105 0.0 26.55799 5 TACATGG 18720 0.0 26.431383 2 CTAGGCA 5315 0.0 26.342276 4 GCTATTG 5185 0.0 26.262291 8 CTATTGA 5195 0.0 26.213804 9 TAGTACT 5035 0.0 26.034246 4 ACATGGG 18545 0.0 25.969294 3 >>END_MODULE