##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3825204
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807173421339098	31.0	31.0	34.0	30.0	34.0
2	31.772977859481482	31.0	31.0	34.0	30.0	34.0
3	31.97259753989591	31.0	31.0	34.0	30.0	34.0
4	35.722035216945294	37.0	35.0	37.0	35.0	37.0
5	35.619053519759994	37.0	35.0	37.0	33.0	37.0
6	35.655359295870234	37.0	35.0	37.0	33.0	37.0
7	35.583671615945185	37.0	35.0	37.0	33.0	37.0
8	35.57126626449204	37.0	35.0	37.0	33.0	37.0
9	37.15255526241215	39.0	37.0	39.0	33.0	39.0
10-11	37.094366601101534	39.0	37.0	39.0	33.0	39.0
12-13	37.01962705779874	39.0	37.0	39.0	33.0	39.0
14-15	38.20676923374545	40.0	37.0	41.0	33.0	41.0
16-17	38.21347279779066	40.0	37.0	41.0	33.0	41.0
18-19	38.2602646813085	40.0	37.5	41.0	33.0	41.0
20-21	38.27291655556148	40.0	38.0	41.0	33.0	41.0
22-23	38.23971401263828	40.0	37.0	41.0	33.0	41.0
24-25	38.16270753141532	40.0	37.0	41.0	33.0	41.0
26-27	38.01459634571124	40.0	37.0	41.0	33.0	41.0
28-29	37.9511226067943	40.0	37.0	41.0	33.0	41.0
30-31	37.82002214783839	40.0	37.0	41.0	33.0	41.0
32-33	37.69617398183208	40.0	36.0	41.0	32.0	41.0
34-35	37.59475324714708	40.0	36.0	41.0	32.0	41.0
36-37	37.50946890675634	40.0	36.0	41.0	32.0	41.0
38-39	37.389256886691534	40.0	36.0	41.0	31.0	41.0
40-41	37.25975059107959	39.5	36.0	41.0	31.0	41.0
42-43	37.13383887499856	39.0	35.0	41.0	31.0	41.0
44-45	36.92609086992485	39.0	35.0	41.0	31.0	41.0
46-47	36.68477681190336	39.0	35.0	41.0	30.0	41.0
48-49	36.427542949343355	39.0	35.0	41.0	30.0	41.0
50-51	36.21957770095399	39.0	35.0	41.0	29.0	41.0
52-53	36.0218083793701	38.0	35.0	40.0	29.0	41.0
54-55	35.81746607501195	38.0	34.5	40.0	29.0	41.0
56-57	35.54164287708577	37.5	34.0	40.0	28.0	41.0
58-59	35.25633181916572	37.0	34.0	40.0	28.0	41.0
60-61	34.98730799716826	36.5	34.0	40.0	28.0	41.0
62-63	34.7032491600448	36.0	34.0	39.0	27.0	41.0
64-65	34.430863817982	35.5	33.0	39.0	27.0	41.0
66-67	34.19642128890381	35.0	33.0	39.0	26.5	41.0
68-69	34.005925043474804	35.0	33.0	38.0	26.0	41.0
70-71	33.80383804367035	35.0	33.0	37.5	26.0	40.0
72-73	33.60279425620176	35.0	33.0	37.0	26.0	40.0
74-75	33.41734989820151	35.0	33.0	36.5	26.0	39.0
76-77	33.27376265422707	35.0	33.0	36.0	26.0	39.0
78-79	33.16406157684662	35.0	33.0	36.0	27.0	39.0
80-81	33.02896629826802	35.0	33.0	35.5	27.0	37.5
82-83	32.84310457690623	35.0	33.0	35.0	26.0	37.0
84-85	32.700708380520354	35.0	33.0	35.0	26.0	37.0
86-87	32.570374155208455	35.0	33.0	35.0	26.0	36.0
88-89	32.46310510498264	35.0	33.0	35.0	26.0	36.0
90-91	32.37316166667189	35.0	33.0	35.0	26.0	36.0
92-93	32.297813005528596	35.0	33.0	35.0	26.0	36.0
94-95	32.259094155501245	35.0	33.0	35.0	26.0	35.0
96-97	32.20657591072267	35.0	33.0	35.0	25.0	35.0
98-99	32.16452312085839	35.0	33.0	35.0	25.0	35.0
100	31.6456581139202	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4707557409230816
1101	2	0.5579137196019026
1101	3	0.4498357401791857
1101	4	0.3065789913646171
1101	5	0.3365564530828564
1101	6	0.41070237852017755
1101	7	0.3735009747692075
1101	8	0.41203753273732246
1101	9	0.5063462706261603
1101	10-11	0.4987342496936975
1101	12-13	0.5121778862304609
1101	14-15	0.6923664344181262
1101	16-17	0.6420828046818841
1101	18-19	0.6180213540439183
1101	20-21	0.6308117336175769
1101	22-23	0.676187777939937
1101	24-25	0.6571356236785277
1101	26-27	0.679163238226586
1101	28-29	0.680205081960537
1101	30-31	0.739094850387211
1101	32-33	0.7582900501709844
1101	34-35	0.7606621048398239
1101	36-37	0.6985512776967369
1101	38-39	0.7496044270272364
1101	40-41	0.7431194173986242
1101	42-43	0.7811448002379748
1101	44-45	0.6950164112298225
1101	46-47	0.7042134180844428
1101	48-49	0.7219390208493195
1101	50-51	0.6821511403923921
1101	52-53	0.779078714136034
1101	54-55	0.7206135565688001
1101	56-57	0.7190137839820494
1101	58-59	0.6749474698615288
1101	60-61	0.7082575106733415
1101	62-63	0.6512147692482699
1101	64-65	0.6545036570048453
1101	66-67	0.7006380871135747
1101	68-69	0.6521271056849614
1101	70-71	0.6824699499265989
1101	72-73	0.5623094004971634
1101	74-75	0.5222976035052298
1101	76-77	0.5571369344816972
1101	78-79	0.5274919625024523
1101	80-81	0.5491584027263485
1101	82-83	0.537368580768522
1101	84-85	0.5035612221617072
1101	86-87	0.4957565703952511
1101	88-89	0.5338693162112094
1101	90-91	0.5554249762400119
1101	92-93	0.5970879279984231
1101	94-95	0.5429824874415559
1101	96-97	0.6052706139182717
1101	98-99	0.5312817021404754
1101	100	0.524051455110147
1102	1	0.4093554023972885
1102	2	0.488871851033192
1102	3	0.3801458863536773
1102	4	0.2995498912462864
1102	5	0.27943672041016754
1102	6	0.30503738952037907
1102	7	0.3162455768538095
1102	8	0.2930632619906035
1102	9	0.4270216536144176
1102	10-11	0.43233501746932035
1102	12-13	0.3815841573128509
1102	14-15	0.522635387026348
1102	16-17	0.55544621530548
1102	18-19	0.5548747333678463
1102	20-21	0.5545858583373757
1102	22-23	0.551835504002625
1102	24-25	0.5568627258311238
1102	26-27	0.5974524955519271
1102	28-29	0.5957227005119563
1102	30-31	0.6086733483275069
1102	32-33	0.6685741586207286
1102	34-35	0.6323927219084027
1102	36-37	0.6748234755370888
1102	38-39	0.6266448182505471
1102	40-41	0.6526038663013409
1102	42-43	0.68885271751396
1102	44-45	0.7140957348710444
1102	46-47	0.7505973310225471
1102	48-49	0.6693699742847627
1102	50-51	0.6733389678079149
1102	52-53	0.6341943336117595
1102	54-55	0.5960149110134978
1102	56-57	0.6968088539028585
1102	58-59	0.6806952210630044
1102	60-61	0.6690023961028331
1102	62-63	0.5915744349134897
1102	64-65	0.5693417905926381
1102	66-67	0.5275989148953713
1102	68-69	0.5869022209531565
1102	70-71	0.6214515262877995
1102	72-73	0.6334884006763488
1102	74-75	0.6337823691736375
1102	76-77	0.6590815123179325
1102	78-79	0.619364196520209
1102	80-81	0.5621580694942168
1102	82-83	0.5384370550934889
1102	84-85	0.5021281227210679
1102	86-87	0.5322434309618771
1102	88-89	0.5255730923582504
1102	90-91	0.5275823457260245
1102	92-93	0.5057362472191969
1102	94-95	0.4679173443401865
1102	96-97	0.46842545867076524
1102	98-99	0.5162477580807803
1102	100	0.5500755797994614
1103	1	0.24693328794909064
1103	2	-0.1339083255429756
1103	3	0.18136041299903738
1103	4	0.19281765782564975
1103	5	0.2295900275732592
1103	6	0.22820897978558463
1103	7	0.23634179538971267
1103	8	0.3134394468986912
1103	9	0.3809454230947793
1103	10-11	0.3546345929477468
1103	12-13	0.4005175044949141
1103	14-15	0.47125850268486147
1103	16-17	0.461569825260014
1103	18-19	0.40893220404151975
1103	20-21	0.4373417972181457
1103	22-23	0.4521381675796263
1103	24-25	0.4781839781956947
1103	26-27	0.5055050361731688
1103	28-29	0.5277303222610783
1103	30-31	0.5256279673082034
1103	32-33	0.5600729244790656
1103	34-35	0.46688786200438415
1103	36-37	0.49983060498064447
1103	38-39	0.4862578113055491
1103	40-41	0.48840762420570627
1103	42-43	0.5548899152348952
1103	44-45	0.5166885753656061
1103	46-47	0.5035083093643635
1103	48-49	0.5072692578058451
1103	50-51	0.5856804085610889
1103	52-53	0.5721316830535415
1103	54-55	0.5433005623629583
1103	56-57	0.6429170915225129
1103	58-59	0.5775887952388246
1103	60-61	0.5717751675877665
1103	62-63	0.5936722374886969
1103	64-65	0.5604098855848108
1103	66-67	0.4773917991316665
1103	68-69	0.5327317196471171
1103	70-71	0.5279264513351478
1103	72-73	0.537307291337342
1103	74-75	0.5217402836220018
1103	76-77	0.42432510103613197
1103	78-79	0.37430061237884615
1103	80-81	0.4029430133881462
1103	82-83	0.3730418510979092
1103	84-85	0.43216147066313226
1103	86-87	0.43451861915928447
1103	88-89	0.3833150588566241
1103	90-91	0.39371516062455925
1103	92-93	0.42546115564927334
1103	94-95	0.4208731199554876
1103	96-97	0.4113998022040235
1103	98-99	0.4349168966497814
1103	100	0.4115604438460423
1104	1	0.3216494111820367
1104	2	0.4412114409748291
1104	3	0.36201820061820555
1104	4	0.2469052608247111
1104	5	0.27651219713986563
1104	6	0.2655524256245769
1104	7	0.28411272510096097
1104	8	0.31692188355631856
1104	9	0.38035911991458704
1104	10-11	0.3433855948899023
1104	12-13	0.3997360157428673
1104	14-15	0.49762211068284756
1104	16-17	0.4917724560701018
1104	18-19	0.4937990369906444
1104	20-21	0.4790200079603437
1104	22-23	0.4308242136624756
1104	24-25	0.4048956477220713
1104	26-27	0.4903748997687032
1104	28-29	0.4926899736083499
1104	30-31	0.5078011527758548
1104	32-33	0.5311957461273167
1104	34-35	0.45554785497866135
1104	36-37	0.5895185096111248
1104	38-39	0.4725845797810422
1104	40-41	0.456827377988823
1104	42-43	0.5008128821750191
1104	44-45	0.5079451604899674
1104	46-47	0.5576527183218758
1104	48-49	0.5477562304304513
1104	50-51	0.42580128507180603
1104	52-53	0.437257527286377
1104	54-55	0.45716453053012174
1104	56-57	0.4651685602289959
1104	58-59	0.5024501730244282
1104	60-61	0.44495579814435615
1104	62-63	0.4795996175277324
1104	64-65	0.38094117133579886
1104	66-67	0.38592303890043667
1104	68-69	0.4143825888827877
1104	70-71	0.44175333513942405
1104	72-73	0.33597365762664566
1104	74-75	0.369778880620828
1104	76-77	0.2585070452211866
1104	78-79	0.3051683730342205
1104	80-81	0.30367622080782297
1104	82-83	0.3092529996666187
1104	84-85	0.3073177393014177
1104	86-87	0.2951796792793928
1104	88-89	0.330870439440865
1104	90-91	0.32731647476061454
1104	92-93	0.37871573397811886
1104	94-95	0.2932437617403991
1104	96-97	0.3214251954378611
1104	98-99	0.3118196387278829
1104	100	0.3228216839238449
1105	1	0.12422008783650895
1105	2	-0.4155534663212457
1105	3	0.03231057509471569
1105	4	0.11456870805967156
1105	5	0.1655856911230984
1105	6	0.2077307193692448
1105	7	0.24132975548602786
1105	8	0.23768187114112038
1105	9	0.3167221793049677
1105	10-11	0.3522761269669914
1105	12-13	0.32783505565147664
1105	14-15	0.4284126030278159
1105	16-17	0.3843528672380998
1105	18-19	0.4137455594740729
1105	20-21	0.38929945428415635
1105	22-23	0.36815853638637464
1105	24-25	0.3874625221491641
1105	26-27	0.40582662147927095
1105	28-29	0.4012227282612244
1105	30-31	0.4385679971213037
1105	32-33	0.43929601190649237
1105	34-35	0.46044833776299754
1105	36-37	0.4737415409658823
1105	38-39	0.46753602168593034
1105	40-41	0.506530024681048
1105	42-43	0.46242241255067285
1105	44-45	0.436573701133085
1105	46-47	0.471091780817801
1105	48-49	0.5120122501649433
1105	50-51	0.5148143694287981
1105	52-53	0.46603459243632983
1105	54-55	0.4396427249590005
1105	56-57	0.5151846588365103
1105	58-59	0.5039238694067194
1105	60-61	0.5114922559116692
1105	62-63	0.47066194884374113
1105	64-65	0.49601724921075174
1105	66-67	0.45734614131511364
1105	68-69	0.49810005730647333
1105	70-71	0.43574561302428094
1105	72-73	0.4614037773533326
1105	74-75	0.42137387443206364
1105	76-77	0.4231471710656294
1105	78-79	0.41067050323789545
1105	80-81	0.3965060951865169
1105	82-83	0.3323589644397913
1105	84-85	0.3227025887584247
1105	86-87	0.32676148121638704
1105	88-89	0.4218688110326596
1105	90-91	0.3854774689503344
1105	92-93	0.3535735489319407
1105	94-95	0.34285871376770416
1105	96-97	0.2543780886807667
1105	98-99	0.3428481180484937
1105	100	0.413921515866555
1106	1	0.27105393268852396
1106	2	0.2942347449108631
1106	3	0.27488812988558564
1106	4	0.1996220993829212
1106	5	0.18318947620638681
1106	6	0.19996236836738035
1106	7	0.1943522617915434
1106	8	0.18478243583501808
1106	9	0.1878606265164322
1106	10-11	0.25791674334715253
1106	12-13	0.30530748349427483
1106	14-15	0.3214569103669973
1106	16-17	0.3206688053773732
1106	18-19	0.3451069585745401
1106	20-21	0.3640937059885516
1106	22-23	0.29668716722623856
1106	24-25	0.25782690380277273
1106	26-27	0.27634723115053816
1106	28-29	0.3023071892000928
1106	30-31	0.3239396102938983
1106	32-33	0.3288773294291474
1106	34-35	0.28950909957024606
1106	36-37	0.29148262359920807
1106	38-39	0.2604402935026542
1106	40-41	0.3217285383006825
1106	42-43	0.3235609464358973
1106	44-45	0.29479884868081285
1106	46-47	0.27490740368844513
1106	48-49	0.23262038885397374
1106	50-51	0.2512846241710136
1106	52-53	0.3212503237123059
1106	54-55	0.29529257123360964
1106	56-57	0.3483490357374208
1106	58-59	0.35167585628786924
1106	60-61	0.31771590256834514
1106	62-63	0.2975103055235948
1106	64-65	0.30273004296376627
1106	66-67	0.24619428205676996
1106	68-69	0.2599861581128806
1106	70-71	0.36029931921435576
1106	72-73	0.2540040022114738
1106	74-75	0.2948609652938856
1106	76-77	0.27782624252676413
1106	78-79	0.26116237108670504
1106	80-81	0.28048339446774406
1106	82-83	0.29272875086244454
1106	84-85	0.21768085459557085
1106	86-87	0.28389893908418884
1106	88-89	0.22745537685240436
1106	90-91	0.18030255470139167
1106	92-93	0.15559212627573515
1106	94-95	0.2088425978439048
1106	96-97	0.20274898360915472
1106	98-99	0.2732358149435825
1106	100	0.39751632556627925
1107	1	0.03367549223339239
1107	2	-0.5031880591261455
1107	3	-0.0638168163675985
1107	4	0.012255059166662363
1107	5	0.07885801583717722
1107	6	0.09306422245849433
1107	7	0.1316094697578407
1107	8	0.1519158010008752
1107	9	0.15808527788864524
1107	10-11	0.1876278132608249
1107	12-13	0.10507104638942621
1107	14-15	0.1598858324857133
1107	16-17	0.24002169691254238
1107	18-19	0.24970829805099726
1107	20-21	0.12391040788800467
1107	22-23	0.17933406503630778
1107	24-25	0.18564594410405277
1107	26-27	0.24289139336631393
1107	28-29	0.140392249207963
1107	30-31	0.19684972224162323
1107	32-33	0.21702988431835024
1107	34-35	0.2692726434975441
1107	36-37	0.25923148172037713
1107	38-39	0.24812270778481604
1107	40-41	0.24526618500240005
1107	42-43	0.29684975719209916
1107	44-45	0.20288303505159888
1107	46-47	0.21600376134141186
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2216	84-85	-0.575826834539491
2216	86-87	-0.5117426904999078
2216	88-89	-0.5650769777997731
2216	90-91	-0.5449499334060377
2216	92-93	-0.5686211396705829
2216	94-95	-0.5659512602764138
2216	96-97	-0.6291751906802148
2216	98-99	-0.6460459761630801
2216	100	-0.6490708080893555
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	12.0
13	68.0
14	294.0
15	860.0
16	2080.0
17	4111.0
18	6882.0
19	10432.0
20	14609.0
21	20108.0
22	27024.0
23	30392.0
24	31606.0
25	33788.0
26	39076.0
27	48606.0
28	61491.0
29	76183.0
30	93367.0
31	115730.0
32	142953.0
33	181060.0
34	246152.0
35	337919.0
36	504809.0
37	850104.0
38	692987.0
39	252193.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83589892687457	22.004169721359705	13.13663550444007	25.023295847325656
2	14.96709296531415	21.20693288240735	39.81591720614126	24.010056946137247
3	17.61941498935852	28.197521284914203	29.983605773089366	24.19945795263791
4	12.233441105742829	18.109138131036048	39.67973707855531	29.977683684665813
5	12.580268954427181	39.10591828886445	35.11275103532998	13.201061721378396
6	27.9863505318932	39.718482988096845	18.81700949805553	13.478156981954426
7	24.78830446720644	32.60831431247675	25.396144031651264	17.207237188665548
8	25.459792471198924	34.593736700055736	20.848770418518857	19.097700410226487
9	26.611704892078958	14.340673072599527	22.071371879774254	36.976250155547255
10-11	25.298859877799984	25.933113632632406	27.736167796541046	21.031858693026567
12-13	25.97848376191178	24.023699128203358	27.980625347040316	22.017191762844543
14-15	23.28336575075627	24.674820283637526	26.045934130951398	25.9958798346548
16-17	21.81602762388186	26.848489554249095	27.380123270108776	23.955359551760267
18-19	21.28685162935101	27.02965645753795	29.81678885622832	21.86670305688272
20-21	22.76466706856265	26.216327922028693	28.834725590962258	22.184279418446398
22-23	23.32405836550292	25.79466719195916	28.50731751695774	22.37395692558018
24-25	22.8329058614667	25.939538372211878	29.29034093092576	21.937214835395665
26-27	22.606606302003872	26.388517085137597	28.345802411170567	22.659074201687964
28-29	21.886166071142874	26.926825340557	28.747316482990186	22.439692105309938
30-31	22.714252625480892	26.48870491612996	28.559535648294837	22.237506810094313
32-33	23.145523742001732	26.714405297076965	27.759892021445133	22.38017893947617
34-35	23.0161889050232	26.340619392831428	28.66863964494234	21.974552057203038
36-37	22.950318466675242	26.113836020248854	28.284543778580172	22.65130173449573
38-39	23.133328313993186	25.89044924139994	28.923882229549065	22.05234021505781
40-41	22.52769014228843	25.33394437083877	29.42020104632766	22.718164440545145
42-43	22.73120124731581	25.566196465833972	28.48733155668213	23.215270730168093
44-45	22.678947318888092	26.622684698646136	28.201737737386033	22.49663024507974
46-47	22.723585461062992	26.292310161758692	27.56433382376469	23.41977055341362
48-49	23.708063896086447	25.64207771164726	27.751991769275143	22.897866622991156
50-51	23.19290683581843	25.986993112001343	27.09743062069369	23.72266943148653
52-53	22.40438679871714	26.480600773187525	27.275473412659824	23.83953901543552
54-55	22.613584301423135	26.511785369998204	26.957567439504597	23.917062889074064
56-57	23.633061848653423	25.796281789673507	26.839508151272067	23.731148210401
58-59	22.383413756269615	26.072442338872225	27.96511758619301	23.579026318665143
60-61	22.62966050672893	26.915735931262176	26.865895413077638	23.58870814893126
62-63	23.15339260337488	26.050793630875635	26.444668049076597	24.35114571667289
64-65	22.047477729292346	26.13885429378407	27.15510074756797	24.65856722935561
66-67	22.977663413506836	25.702589979514816	26.97253532099203	24.34721128598632
68-69	22.21354136370986	25.751497360950747	27.318365453453875	24.716595821885516
70-71	22.218268873193132	25.485812059210478	27.468640262158456	24.82727880543793
72-73	22.840298190632446	25.686839185570232	27.64625102347483	23.826611600322494
74-75	22.82385305535364	26.17038789259268	27.20991646047895	23.795842591574733
76-77	22.38319160678353	26.062060255330007	26.894746762755062	24.6600013751314
78-79	22.173899233409927	26.199833655169492	26.978357787260833	24.64790932415975
80-81	21.797670472177348	26.488699413631185	27.64300599179047	24.070624122400993
82-83	22.607622886384	25.339066109754445	28.460848089441974	23.59246291441958
84-85	22.973797740101325	25.863252875265573	26.943071421409886	24.219877963223215
86-87	21.487848328821677	26.63071981376797	27.466544275859327	24.414887581551024
88-89	22.38541404541934	25.343520930217355	27.341828081034535	24.92923694332877
90-91	22.004844750846708	25.306770821010232	27.521209673972137	25.167174754170922
92-93	22.228560851186653	25.730782624882607	27.248611195355032	24.7920453285757
94-95	21.03074114338769	25.664990174896385	27.827166538937487	25.477102142778435
96-97	21.392902156615612	25.452017126180483	27.461640079884532	25.693440637319377
98-99	20.86867260625703	26.29743850100526	27.33685592112255	25.497032971615162
100	21.64836809460242	25.323850973345184	26.594006134003006	26.433774798049388
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	188.5
2	148.0
3	199.5
4	156.5
5	139.5
6	247.0
7	571.0
8	920.0
9	988.0
10	751.0
11	527.5
12	525.5
13	574.0
14	659.0
15	878.0
16	1121.5
17	1416.0
18	1829.0
19	2349.0
20	3026.0
21	3668.5
22	4354.0
23	5167.0
24	5864.5
25	6556.0
26	7725.0
27	8915.0
28	10983.5
29	16713.0
30	25485.5
31	34128.5
32	45831.5
33	59386.5
34	73441.5
35	85979.5
36	98414.5
37	122619.5
38	131897.0
39	120743.0
40	110617.0
41	101559.0
42	100006.5
43	105785.0
44	115635.0
45	138185.5
46	160347.5
47	176956.0
48	188616.5
49	183132.5
50	172521.5
51	172350.0
52	171646.0
53	165781.0
54	159385.5
55	139934.5
56	123031.0
57	101247.5
58	78642.0
59	74216.5
60	60828.5
61	41962.0
62	30665.5
63	24298.5
64	17583.0
65	8807.0
66	4692.5
67	3111.0
68	2226.0
69	1625.0
70	1258.0
71	943.0
72	698.5
73	578.5
74	441.0
75	301.5
76	190.5
77	95.5
78	38.0
79	17.5
80	10.0
81	5.0
82	1.5
83	1.5
84	1.0
85	0.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012757489535198646
2	0.004862485765465057
3	0.0020129645373161797
4	0.02865206666101991
5	0.0024835276759095724
6	0.0
7	4.4442074200487085E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009345906780396549
16-17	0.002823378831560356
18-19	0.0
20-21	5.228479317704363E-5
22-23	8.757702857154808E-4
24-25	2.6142396588521813E-5
26-27	2.091391727081745E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.882039232417408E-4
36-37	0.0
38-39	0.0
40-41	4.18278345416349E-4
42-43	7.842718976556545E-4
44-45	0.0
46-47	0.0
48-49	6.404887164187845E-4
50-51	0.0
52-53	0.0
54-55	4.313495437106099E-4
56-57	9.149838805982635E-5
58-59	2.6142396588521816E-4
60-61	7.842718976556544E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015031878038400043
70-71	9.541974754810463E-4
72-73	0.0
74-75	2.2221037100243543E-4
76-77	0.0030063756076800087
78-79	4.574919402991317E-4
80-81	0.0035815083326274887
82-83	0.0016992557782539181
84-85	0.00547683208529532
86-87	0.007790434183379501
88-89	0.007620508605554109
90-91	0.006261103982950974
92-93	0.008679275667389243
94-95	0.0013986182174859172
96-97	0.004784058575699492
98-99	0.002104462925376006
100	0.08443994098092546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3825204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.23715439104344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89295379383152	14.63090296174622
2	15.211429153713574	6.4609493889166885
3	5.955570760224077	3.7943812716498804
4	2.922080235253676	2.482266763963954
5	1.5957574048109786	1.6944673188310777
6	1.019990756812571	1.2997020707919489
7	0.6861171638492018	1.0199823297307242
8	0.4794202309901941	0.8145217170982769
9	0.3729538654054675	0.7128430938317113
>10	2.043714679995675	8.323347152664358
>50	0.2976355906703488	4.488664392181171
>100	0.387697165766889	18.5226373880533
>500	0.07768059813941482	11.725995413295458
>1k	0.05478267222193279	20.393116208166816
>5k	0.0017234998002405822	2.3562947876485287
>10k+	4.924285143544521E-4	1.2799277414300134
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12972	0.33911916854630497	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12652	0.330753601637978	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11817	0.3089247004865623	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11515	0.30102969671682867	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9418	0.24620909107069844	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	8922	0.2332424623627916	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7600	0.1986822140727658	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7382	0.19298317161646805	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6890	0.1801211124949153	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6446	0.16851388840961162	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5854	0.15303758962920672	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5735	0.14992664443517262	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5601	0.14642356329231068	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5538	0.1447765923072338	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5435	0.14208392545861606	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5179	0.13539147193195447	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5078	0.13275108987651377	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5048	0.13196681797885812	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4910	0.1283591672496421	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4900	0.1280977432837569	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4765	0.12456851974430645	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4607	0.12043802108332	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4552	0.11900018927095131	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4529	0.11839891414941531	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4518	0.11811134778694156	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4461	0.1166212311813958	No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC	4454	0.11643823440527616	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4352	0.11377170995324694	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4277	0.11181103020910779	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4267	0.11154960624322258	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4226	0.1104777679830932	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4107	0.1073668227890591	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4082	0.10671326287434604	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	4014	0.10493557990632657	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4010	0.10483101031997248	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	3933	0.1028180457826563	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3911	0.10224291305770883	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3905	0.10208605867817767	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6142396588521813E-5
2	0.0	0.0	0.0	0.0	2.6142396588521813E-5
3	0.0	0.0	0.0	0.0	2.6142396588521813E-5
4	0.0	0.0	0.0	0.0	2.6142396588521813E-5
5	0.0	0.0	0.0	0.0	2.6142396588521813E-5
6	0.0	0.0	0.0	0.0	5.228479317704363E-5
7	0.0	0.0	0.0	0.0	5.228479317704363E-5
8	0.0	0.0	0.0	0.0	5.228479317704363E-5
9	0.0	0.0	0.0	0.0	5.228479317704363E-5
10-11	0.0	0.0	0.0	1.3071198294260907E-5	7.842718976556544E-5
12-13	0.0	0.0	0.0	2.6142396588521813E-5	1.0456958635408725E-4
14-15	0.0	0.0	0.0	2.6142396588521813E-5	1.0456958635408725E-4
16-17	0.0	0.0	0.0	2.6142396588521813E-5	1.0456958635408725E-4
18-19	0.0	0.0	0.0	3.921359488278272E-5	1.1764078464834816E-4
20-21	0.0	0.0	0.0	7.842718976556544E-5	1.829967761196527E-4
22-23	0.0	0.0	0.0	1.699255778253918E-4	2.091391727081745E-4
24-25	0.0	0.0	0.0	4.967055351819145E-4	2.091391727081745E-4
26-27	0.0	0.0	0.0	0.0011633366481892207	2.3528156929669635E-4
28-29	0.0	0.0	0.0	0.0037252915138643585	2.3528156929669635E-4
30-31	0.0	0.0	0.0	0.01297969990620108	2.3528156929669635E-4
32-33	0.0	0.0	0.0	0.035945795309217494	2.3528156929669635E-4
34-35	0.0	0.0	0.0	0.07139488508325308	2.6142396588521816E-4
36-37	1.3071198294260907E-5	0.0	0.0	0.12788860411104871	2.6142396588521816E-4
38-39	2.6142396588521813E-5	0.0	0.0	0.21975298572311436	2.8756636247373994E-4
40-41	2.6142396588521813E-5	0.0	0.0	0.3275903716507669	2.8756636247373994E-4
42-43	2.6142396588521813E-5	0.0	0.0	0.4431397645720333	2.8756636247373994E-4
44-45	2.6142396588521813E-5	0.0	0.0	0.57734175745921	3.137087590622618E-4
46-47	2.6142396588521813E-5	0.0	0.0	0.7388756259796863	3.398511556507836E-4
48-49	2.6142396588521813E-5	0.0	0.0	0.9210619878050949	3.659935522393054E-4
50-51	2.6142396588521813E-5	0.0	0.0	1.128175124777659	3.659935522393054E-4
52-53	2.6142396588521813E-5	0.0	0.0	1.3404513850764561	3.659935522393054E-4
54-55	2.6142396588521813E-5	0.0	0.0	1.5605965067483982	3.9213594882782723E-4
56-57	2.6142396588521813E-5	0.0	0.0	1.8260856153031315	3.9213594882782723E-4
58-59	2.6142396588521813E-5	0.0	0.0	2.143990229017851	3.9213594882782723E-4
60-61	2.6142396588521813E-5	0.0	0.0	2.4881025953125637	3.9213594882782723E-4
62-63	2.6142396588521813E-5	0.0	0.0	2.849978720089177	4.4442074200487085E-4
64-65	2.6142396588521813E-5	0.0	0.0	3.2251090399361706	4.4442074200487085E-4
66-67	2.6142396588521813E-5	0.0	0.0	3.639557524252301	4.4442074200487085E-4
68-69	2.6142396588521813E-5	0.0	0.0	4.090827574163365	4.4442074200487085E-4
70-71	2.6142396588521813E-5	0.0	0.0	4.599950747724828	4.4442074200487085E-4
72-73	2.6142396588521813E-5	0.0	0.0	5.145385710147746	4.5749194029913177E-4
74-75	2.6142396588521813E-5	0.0	0.0	5.682311322481102	4.705631385933927E-4
76-77	2.6142396588521813E-5	0.0	0.0	6.285246486200474	4.967055351819145E-4
78-79	2.6142396588521813E-5	0.0	0.0	6.926689400094739	5.228479317704363E-4
80-81	2.6142396588521813E-5	0.0	0.0	7.631932832863293	5.620615266532191E-4
82-83	2.6142396588521813E-5	0.0	0.0	8.384075725111654	6.535599147130454E-4
84-85	2.6142396588521813E-5	0.0	0.0	9.12058546420008	6.535599147130454E-4
86-87	2.6142396588521813E-5	0.0	0.0	9.883316027066792	6.92773509595828E-4
88	2.6142396588521813E-5	0.0	0.0	10.470421969651815	7.05844707890089E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7415	0.0	40.647877	1
GTATCAA	12895	0.0	40.11089	1
GGGCACC	3470	0.0	34.70566	94
CAACGCA	15300	0.0	32.49853	5
ATCAACG	15590	0.0	31.904436	3
TATCAAC	16500	0.0	31.373306	2
TATTCTC	2235	0.0	30.490183	5
AACGCAG	17865	0.0	28.857328	6
GAACCGT	540	0.0	27.848942	6
AGGGCTA	5280	0.0	27.5929	5
TATTCGC	495	0.0	27.537518	9
ACGCAGA	18155	0.0	27.179758	7
CGCAGAG	18320	0.0	26.883654	8
TAGGCAT	4795	0.0	26.561354	5
GCTATTG	5495	0.0	26.512245	8
TAGTACT	5030	0.0	26.449915	4
CTGTGCG	5055	0.0	26.407612	9
CTATTGA	5505	0.0	26.383545	9
GTACATG	16840	0.0	26.051369	1
TCAACGC	19470	0.0	25.932484	4
>>END_MODULE