FastQCFastQC Report
Mon 16 Jan 2017
ERR1146464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918711
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120380.30719284989375334No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC88680.22629890287903343No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT74900.19113427859313942No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66070.16860135896727266No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC58220.14856926167813855No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57120.1457622161981325No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53630.13685622644793147No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45830.11695172213516128No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG40600.10360549680749613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65200.031.5419121
GGTATCA43500.031.228591
CAACGCA61850.030.689695
GGGCACC32700.029.15368894
GTACTAG27750.029.06581
ATCAACG65700.028.8253213
GTACATG136500.027.1260221
TAGTACT39850.026.6490154
AACGCAG76100.026.5287636
CTAGTAC42850.026.210883
TACATGG138100.026.2038462
GCATCGT29500.026.102888
CTGTGCG39450.026.0673649
ACATGGG138850.025.417253
TATCAAC80100.025.3465672
TAGGCAT38050.024.6959765
GGCGTCG20350.024.6880048
ACGCAGA77850.024.0644137
TCTAACG13450.023.7604472
CTAACGC13500.023.670623
Produced by FastQC (version 0.11.5)