##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146464_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3918711
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.377213068276788	31.0	30.0	34.0	26.0	34.0
2	30.48327677136691	31.0	30.0	34.0	26.0	34.0
3	30.691401075506715	31.0	31.0	34.0	27.0	34.0
4	34.14901354042184	37.0	35.0	37.0	30.0	37.0
5	34.33973084516822	37.0	35.0	37.0	30.0	37.0
6	34.530567321754525	37.0	35.0	37.0	30.0	37.0
7	34.42610006198467	37.0	35.0	37.0	30.0	37.0
8	34.369684827485365	37.0	35.0	37.0	30.0	37.0
9	35.74589399422412	39.0	35.0	39.0	30.0	39.0
10-11	35.673348072873964	39.0	35.0	39.0	29.5	39.0
12-13	35.66790049074811	39.0	35.0	39.0	30.0	39.0
14-15	36.6902583528104	40.0	36.0	41.0	29.5	41.0
16-17	36.51078198928168	40.0	36.0	41.0	28.0	41.0
18-19	36.45713258773102	40.0	36.0	41.0	27.5	41.0
20-21	36.785931521870324	40.0	36.0	41.0	29.5	41.0
22-23	36.50702922466087	40.0	36.0	41.0	28.0	41.0
24-25	36.617842831482086	39.5	36.0	41.0	29.0	41.0
26-27	36.694277276379914	40.0	36.0	41.0	29.5	41.0
28-29	36.52202726866054	39.5	35.5	41.0	28.5	41.0
30-31	36.4572897822779	39.0	35.0	41.0	28.5	41.0
32-33	36.55522747148233	39.0	35.0	41.0	29.5	41.0
34-35	36.60442413334385	40.0	35.0	41.0	29.5	41.0
36-37	36.53568788308196	39.0	35.0	41.0	29.0	41.0
38-39	36.37794532436814	39.0	35.0	41.0	29.0	41.0
40-41	36.348014436379714	39.0	35.0	41.0	28.5	41.0
42-43	36.19474464945233	39.0	35.0	41.0	28.0	41.0
44-45	36.03666869029127	39.0	35.0	41.0	28.0	41.0
46-47	35.92307661881675	39.0	34.5	41.0	27.5	41.0
48-49	35.81398577236239	39.0	34.0	40.5	27.0	41.0
50-51	35.61512675979423	38.0	34.0	40.0	27.0	41.0
52-53	35.45188736806567	38.0	34.0	40.0	27.0	41.0
54-55	35.26680533471338	38.0	33.5	40.0	26.0	41.0
56-57	35.149194850041255	38.0	33.0	40.0	26.0	41.0
58-59	34.918902924966915	37.0	33.0	40.0	26.0	41.0
60-61	34.674345849949134	37.0	33.0	40.0	26.0	41.0
62-63	34.41747094899318	36.0	33.0	40.0	26.0	41.0
64-65	34.13930869104662	36.0	32.5	39.0	26.0	41.0
66-67	33.84859409127134	35.0	32.0	39.0	26.0	41.0
68-69	33.564593561505305	35.0	32.0	38.5	25.0	40.5
70-71	33.4660791520477	35.0	32.0	38.0	25.5	40.0
72-73	33.234915766944795	35.0	32.0	37.0	25.0	40.0
74-75	32.95758528761115	35.0	32.0	37.0	25.0	39.5
76-77	32.70556734089347	35.0	32.0	36.0	25.0	39.0
78-79	32.47467891865463	35.0	31.0	36.0	24.5	39.0
80-81	32.2255773645977	35.0	31.0	36.0	24.0	37.5
82-83	31.99719486841464	35.0	31.0	35.0	24.0	37.0
84-85	31.80019616144186	35.0	31.0	35.0	24.0	37.0
86-87	31.62028929921089	34.5	31.0	35.0	24.0	36.0
88-89	31.598714475244538	35.0	31.0	35.0	24.0	36.0
90-91	31.52193808117006	35.0	31.0	35.0	23.5	36.0
92-93	31.38377734413178	35.0	31.0	35.0	23.0	36.0
94-95	31.346453463906883	35.0	31.0	35.0	23.0	35.0
96-97	31.261554500957075	35.0	31.0	35.0	23.0	35.0
98-99	31.176279776691878	34.0	31.0	35.0	23.0	35.0
100	30.692121210265313	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7766990149022881
1101	2	0.9291183277818078
1101	3	0.9197578901993317
1101	4	0.9835642319481579
1101	5	0.905446763862777
1101	6	0.6090689444962436
1101	7	0.6517133668295685
1101	8	0.6631570502167321
1101	9	0.825538418835059
1101	10-11	0.8640282667308057
1101	12-13	0.7415873674428894
1101	14-15	0.9322543267658361
1101	16-17	1.1141808804354554
1101	18-19	1.2383862715816747
1101	20-21	0.9080938433459096
1101	22-23	1.1824954155500365
1101	24-25	1.081749343663546
1101	26-27	0.9667876168161555
1101	28-29	1.1579349651566488
1101	30-31	1.1865593184611
1101	32-33	1.0513747319267353
1101	34-35	0.9389375735079213
1101	36-37	0.9063802842269908
1101	38-39	0.9727021522340564
1101	40-41	0.9716971629464268
1101	42-43	0.942658615262836
1101	44-45	0.9512521526593787
1101	46-47	0.9833295991608395
1101	48-49	0.8706455168589713
1101	50-51	0.9509830007395408
1101	52-53	0.9592657444005184
1101	54-55	0.9678887173380772
1101	56-57	0.8561522104281849
1101	58-59	0.7807487805579001
1101	60-61	0.8120997357162238
1101	62-63	0.7910502684115102
1101	64-65	0.724980598791575
1101	66-67	0.7883016757087375
1101	68-69	0.7790120301264594
1101	70-71	0.7385209765856757
1101	72-73	0.7281500316786662
1101	74-75	0.6925799745613119
1101	76-77	0.7228775412454951
1101	78-79	0.6682868829881841
1101	80-81	0.7401046392334649
1101	82-83	0.664771624482519
1101	84-85	0.6116309963434112
1101	86-87	0.6482649263321321
1101	88-89	0.6250372224263927
1101	90-91	0.6236784553326267
1101	92-93	0.6565063857262317
1101	94-95	0.6432359423178511
1101	96-97	0.6183059783259175
1101	98-99	0.6316592010062685
1101	100	0.6840679936556704
1102	1	0.6515430562089719
1102	2	0.8078142489905602
1102	3	0.7851704061773503
1102	4	0.8679524371114837
1102	5	0.7389816278315635
1102	6	0.4765041026071515
1102	7	0.5417591103765247
1102	8	0.5831735589703797
1102	9	0.7596290791361469
1102	10-11	0.7264174572915323
1102	12-13	0.7037709601669064
1102	14-15	0.9174815100852314
1102	16-17	1.0850452556525028
1102	18-19	1.0749975008135735
1102	20-21	0.8541659663157404
1102	22-23	1.0615386705064438
1102	24-25	0.937051047559784
1102	26-27	0.8460180967367847
1102	28-29	0.9969365921003899
1102	30-31	1.004624584248866
1102	32-33	0.9445421563332346
1102	34-35	0.8176056332136028
1102	36-37	0.7692962550449423
1102	38-39	0.8360621318788972
1102	40-41	0.785937041652403
1102	42-43	0.8331313471853932
1102	44-45	0.844565755097797
1102	46-47	0.7958649799496698
1102	48-49	0.7576235950868551
1102	50-51	0.8457664600806822
1102	52-53	0.7841172917804258
1102	54-55	0.7945358952266517
1102	56-57	0.7042659853152102
1102	58-59	0.6665657279350796
1102	60-61	0.7001455355824362
1102	62-63	0.671420129322712
1102	64-65	0.6490917728018601
1102	66-67	0.6858818418310477
1102	68-69	0.7087965569046588
1102	70-71	0.6395698320836374
1102	72-73	0.575123951279096
1102	74-75	0.5838175291355086
1102	76-77	0.5843146977416822
1102	78-79	0.610560721505685
1102	80-81	0.6502540969545691
1102	82-83	0.6325295369664516
1102	84-85	0.6047717002675448
1102	86-87	0.6579222165640886
1102	88-89	0.5625274197441072
1102	90-91	0.6066889552436088
1102	92-93	0.7150743684306349
1102	94-95	0.6500381331534797
1102	96-97	0.6787156253550926
1102	98-99	0.7050691527503083
1102	100	0.7194567642069494
1103	1	0.6188941772715175
1103	2	0.7709762705898626
1103	3	0.7105918370563309
1103	4	0.6782366926608461
1103	5	0.6940085618246954
1103	6	0.5378414480002647
1103	7	0.6183926155236037
1103	8	0.666877225671918
1103	9	0.7201445006838583
1103	10-11	0.8571107365263231
1103	12-13	0.6857028257110969
1103	14-15	0.841957441518943
1103	16-17	1.070514131428837
1103	18-19	1.1706983452415116
1103	20-21	0.8491667219967383
1103	22-23	1.0810403234372075
1103	24-25	0.9566765848032048
1103	26-27	0.8508876417651905
1103	28-29	0.992279074208156
1103	30-31	1.0962812911579292
1103	32-33	0.8359911021974895
1103	34-35	0.8600693432935458
1103	36-37	0.7664748330707312
1103	38-39	0.9072231306807979
1103	40-41	0.7840843258088697
1103	42-43	0.8618181103980831
1103	44-45	0.9019648168610814
1103	46-47	0.8224167281036898
1103	48-49	0.7790702716459919
1103	50-51	0.8290487444722743
1103	52-53	0.8478360901989319
1103	54-55	0.8251454201498731
1103	56-57	0.6827410793388751
1103	58-59	0.6494952414337973
1103	60-61	0.699488522914649
1103	62-63	0.729171549474728
1103	64-65	0.743031823280127
1103	66-67	0.7219419305396713
1103	68-69	0.6728573984466735
1103	70-71	0.6311038096916874
1103	72-73	0.6490850282917862
1103	74-75	0.6125393951212175
1103	76-77	0.5986051058304511
1103	78-79	0.5979176288039341
1103	80-81	0.6805026807413057
1103	82-83	0.6193155118996643
1103	84-85	0.6093616473177086
1103	86-87	0.6482503779912818
1103	88-89	0.7297919468697032
1103	90-91	0.7217976677864719
1103	92-93	0.7010180364905096
1103	94-95	0.7409034974123472
1103	96-97	0.6699547157179602
1103	98-99	0.7845934737159048
1103	100	0.7835961305723558
1104	1	0.4918980557094663
1104	2	0.7352071743466517
1104	3	0.6749862010020848
1104	4	0.7023903503333315
1104	5	0.6857408728383803
1104	6	0.44363347669962394
1104	7	0.5036976161820874
1104	8	0.48760696025051686
1104	9	0.6377420169593577
1104	10-11	0.5320128335586816
1104	12-13	0.5137094045591297
1104	14-15	0.6624809668234519
1104	16-17	0.8814176398444005
1104	18-19	0.8585294532917018
1104	20-21	0.6898244958807211
1104	22-23	0.9412323992428284
1104	24-25	0.7930317335881938
1104	26-27	0.6406124546795624
1104	28-29	0.8143963628908253
1104	30-31	0.8913244511490745
1104	32-33	0.7124929888756881
1104	34-35	0.5880058598141531
1104	36-37	0.6003944117722568
1104	38-39	0.7476282577369986
1104	40-41	0.5454993332639546
1104	42-43	0.5834739610508706
1104	44-45	0.5746529968876501
1104	46-47	0.5318289727631509
1104	48-49	0.4961834150472555
1104	50-51	0.6384456859676177
1104	52-53	0.6621825451839527
1104	54-55	0.6208860854652158
1104	56-57	0.5849752218894864
1104	58-59	0.5652854877271736
1104	60-61	0.6176649695379837
1104	62-63	0.5211572741992256
1104	64-65	0.4871633305048704
1104	66-67	0.6312956001956991
1104	68-69	0.5945709006589723
1104	70-71	0.4612206834614412
1104	72-73	0.5404918969685326
1104	74-75	0.5165942612766372
1104	76-77	0.5750318512396433
1104	78-79	0.5187805277114848
1104	80-81	0.5387332608150786
1104	82-83	0.5357255668559873
1104	84-85	0.5060364849594201
1104	86-87	0.5328862665726746
1104	88-89	0.4520609987849511
1104	90-91	0.5072946731347763
1104	92-93	0.3987765647671466
1104	94-95	0.4265498910440684
1104	96-97	0.36408678948085793
1104	98-99	0.44304769439464664
1104	100	0.5474333075596967
1105	1	0.45220944067509095
1105	2	0.6158976047778104
1105	3	0.6107239588691087
1105	4	0.7043520087543058
1105	5	0.573600253527502
1105	6	0.27876967459526725
1105	7	0.38480094928939934
1105	8	0.3829981818290804
1105	9	0.36398506228577077
1105	10-11	0.5013777466448914
1105	12-13	0.4345974540125468
1105	14-15	0.5906515114006936
1105	16-17	0.7359753480148186
1105	18-19	0.7660092600861432
1105	20-21	0.5810850460967885
1105	22-23	0.8089788767300661
1105	24-25	0.7277309124874733
1105	26-27	0.517271725584834
1105	28-29	0.8128896388320754
1105	30-31	0.7545912109468844
1105	32-33	0.6531859023846422
1105	34-35	0.631004584709224
1105	36-37	0.5121027841255454
1105	38-39	0.6851069470116258
1105	40-41	0.5228041504753236
1105	42-43	0.5866079772890131
1105	44-45	0.6694507601764599
1105	46-47	0.5866146334067608
1105	48-49	0.6631771910415551
1105	50-51	0.7065735356262905
1105	52-53	0.6539475969558524
1105	54-55	0.6225186484557881
1105	56-57	0.6516113645831538
1105	58-59	0.6433243900881607
1105	60-61	0.6141244403027102
1105	62-63	0.5815459149675632
1105	64-65	0.545043226916583
1105	66-67	0.6304204702958174
1105	68-69	0.5097812619683566
1105	70-71	0.5011733513074574
1105	72-73	0.4452260964313979
1105	74-75	0.47744557411976984
1105	76-77	0.5603812069402707
1105	78-79	0.5187885619914354
1105	80-81	0.6078800620730078
1105	82-83	0.6020796470097949
1105	84-85	0.528756606368713
1105	86-87	0.5422049663727506
1105	88-89	0.5479439695219739
1105	90-91	0.5634373721918102
1105	92-93	0.6920885928795713
1105	94-95	0.4900120483048518
1105	96-97	0.5545093211606584
1105	98-99	0.5736916783298618
1105	100	0.6346619163904244
1106	1	0.5772892933900806
1106	2	0.5521883719972287
1106	3	0.5519788948702669
1106	4	0.6467551171266592
1106	5	0.53439465387045
1106	6	0.3229037084857538
1106	7	0.37668373997095017
1106	8	0.40819117172407005
1106	9	0.5387576712211413
1106	10-11	0.470336091228603
1106	12-13	0.4394194846282602
1106	14-15	0.5875925407290197
1106	16-17	0.672525590723069
1106	18-19	0.6710384310779531
1106	20-21	0.5338165723020154
1106	22-23	0.8006469267770981
1106	24-25	0.6089927420563512
1106	26-27	0.4763880688738382
1106	28-29	0.777624306477442
1106	30-31	0.6736807933260209
1106	32-33	0.6001937673187214
1106	34-35	0.535112457710234
1106	36-37	0.5072247516057757
1106	38-39	0.6317005311934878
1106	40-41	0.5779649592761587
1106	42-43	0.5095484239308661
1106	44-45	0.5009675650811616
1106	46-47	0.5392630901209543
1106	48-49	0.5041764516830867
1106	50-51	0.5009964793397046
1106	52-53	0.4768317083489748
1106	54-55	0.4632725674223721
1106	56-57	0.5122909610227637
1106	58-59	0.5444643621805412
1106	60-61	0.5122121086167652
1106	62-63	0.4939606704025721
1106	64-65	0.5190250756327757
1106	66-67	0.5864471085348555
1106	68-69	0.5556531549460502
1106	70-71	0.5236947837028225
1106	72-73	0.4823884266172911
1106	74-75	0.4863196091703017
1106	76-77	0.4736899927723144
1106	78-79	0.5320138783188568
1106	80-81	0.5959221888386566
1106	82-83	0.5114917003529413
1106	84-85	0.500497512226179
1106	86-87	0.567457891638874
1106	88-89	0.633091464536804
1106	90-91	0.5792361760178117
1106	92-93	0.6187126314302205
1106	94-95	0.5708919315542147
1106	96-97	0.624320561409494
1106	98-99	0.6677310090004376
1106	100	0.6014837216651365
1107	1	0.5849176085744325
1107	2	0.6520850690079101
1107	3	0.5528271015237713
1107	4	0.7117355357644968
1107	5	0.5947818189525904
1107	6	0.4020774957977693
1107	7	0.39687845125394716
1107	8	0.4910810943029347
1107	9	0.635885056018509
1107	10-11	0.6394010374549453
1107	12-13	0.5233033601867731
1107	14-15	0.6732799610652549
1107	16-17	0.8205000930369906
1107	18-19	0.9024111198798366
1107	20-21	0.6636805754017701
1107	22-23	0.9270142061988125
1107	24-25	0.6598642826438663
1107	26-27	0.5155226076507446
1107	28-29	0.6780074186499618
1107	30-31	0.6979783164965809
1107	32-33	0.6737233169242884
1107	34-35	0.5079795480181772
1107	36-37	0.4794211315785901
1107	38-39	0.5327132305303053
1107	40-41	0.34487862900098776
1107	42-43	0.5200828865341904
1107	44-45	0.5173185470536339
1107	46-47	0.519962131218378
1107	48-49	0.4161194653976281
1107	50-51	0.5390876249203558
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2209	24-25	0.43996706015097686
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2209	58-59	0.25067875765005
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2210	3	0.015990485093674067
2210	4	0.16567723038182436
2210	5	0.07148218435047937
2210	6	0.08596143380612631
2210	7	-0.43932652873716194
2210	8	-0.2074803011858748
2210	9	-0.0807902969616876
2210	10-11	0.04399446591599343
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2210	16-17	0.24229173241249669
2210	18-19	0.2393008955074194
2210	20-21	-0.3683331455025538
2210	22-23	-1.6880638196320774
2210	24-25	-0.775777449356724
2210	26-27	-0.10664779751501641
2210	28-29	0.21751360563080624
2210	30-31	0.20481035373821754
2210	32-33	0.12631553068985824
2210	34-35	0.07607895311416968
2210	36-37	0.12011112598672469
2210	38-39	0.0656879692380059
2210	40-41	0.017311237612886998
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2210	46-47	0.02288713665926423
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2210	60-61	-0.05145940058192622
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2210	64-65	-0.05338714591407978
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2211	5	-0.4085705327788034
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2211	8	-0.22663213542013239
2211	9	-0.18101792184589272
2211	10-11	-0.2145042733709417
2211	12-13	-0.25729025092624624
2211	14-15	-0.38065952654639545
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2211	18-19	-0.36678431598488004
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2211	22-23	-1.9871685293257002
2211	24-25	-1.2330555877169616
2211	26-27	-0.5082238546875999
2211	28-29	-0.19536419903581503
2211	30-31	-0.12219568383944335
2211	32-33	-0.2904169953862379
2211	34-35	-0.3269695854622867
2211	36-37	-0.3238197357298631
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2211	40-41	-0.2398250103432602
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2211	46-47	-0.27952527304562835
2211	48-49	-0.26181199970750413
2211	50-51	-0.2783974092487256
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2211	54-55	-0.19366574752892518
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2211	64-65	-0.3040640315644154
2211	66-67	-1.303407510400234
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2211	70-71	-0.348493723763724
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2211	84-85	-0.2829629144096053
2211	86-87	-0.16556998098920417
2211	88-89	-0.2544891344830482
2211	90-91	-0.19059530988798556
2211	92-93	-0.29787955892745543
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2212	32-33	-0.4193630289340575
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2212	58-59	-0.47021101544265065
2212	60-61	-0.3590828306397924
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2212	64-65	-0.2528333404234857
2212	66-67	-0.3576623241929866
2212	68-69	-0.2908759893783568
2212	70-71	-0.2138621353680179
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2212	74-75	-0.21223175036188024
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2212	86-87	-0.2696978738808262
2212	88-89	-0.40856377105290065
2212	90-91	-0.3599270391611462
2212	92-93	-0.20727370395099598
2212	94-95	-0.31008776030801855
2212	96-97	-0.26825580320424436
2212	98-99	-0.32289604244925485
2212	100	-0.35077152192990724
2213	1	-0.5758307236852644
2213	2	-0.3781265383781367
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2213	4	-0.5175100414271299
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2213	6	-1.028539508668274
2213	7	-0.6172111441217751
2213	8	-0.6411899778065688
2213	9	-0.6962176207965527
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2213	14-15	-0.5134036649242546
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2213	20-21	-1.4770994985062558
2213	22-23	-0.7185288713278766
2213	24-25	-0.5336104267175301
2213	26-27	-3.262346610921597
2213	28-29	-1.020211325540508
2213	30-31	-1.4059810400345185
2213	32-33	-0.8955926663615656
2213	34-35	-0.7980100364410347
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2213	40-41	-0.6310793759363023
2213	42-43	-0.7430309915943027
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2213	46-47	-0.7192766880041859
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2213	56-57	-0.7162595624328176
2213	58-59	-0.7406442394198507
2213	60-61	-0.7815206407858071
2213	62-63	-0.7205451464099397
2213	64-65	-0.6144151486276073
2213	66-67	-0.6571652659634566
2213	68-69	-0.6155634843079909
2213	70-71	-0.7052988015337007
2213	72-73	-0.6845673150720799
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2213	78-79	-0.5469426292499548
2213	80-81	-0.522095138137459
2213	82-83	-0.4577546826453762
2213	84-85	-0.5509296313992351
2213	86-87	-0.4282783989098391
2213	88-89	-0.512920900963465
2213	90-91	-0.5664514231175417
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2213	94-95	-0.5629523411951247
2213	96-97	-0.5166905825549186
2213	98-99	-0.5328123782792709
2213	100	-0.4606568538325817
2214	1	-1.003405256189808
2214	2	-0.9045536411859274
2214	3	-1.1441023842654978
2214	4	-0.8565751101579622
2214	5	-1.0768549380053472
2214	6	-0.9404373005628486
2214	7	-1.1465297025310548
2214	8	-0.9247685085847053
2214	9	-4.464420962342594
2214	10-11	-1.637342935392681
2214	12-13	-1.3122278139371986
2214	14-15	-3.580481848144302
2214	16-17	-3.3076261619427356
2214	18-19	-1.7503770132029146
2214	20-21	-1.6459664943680963
2214	22-23	-2.9185876606979093
2214	24-25	-2.252377614847852
2214	26-27	-1.421954003118941
2214	28-29	-1.115109460113267
2214	30-31	-1.1110029427008996
2214	32-33	-1.2490119385391338
2214	34-35	-1.1236396127129566
2214	36-37	-1.2419858550538834
2214	38-39	-1.1068006976494544
2214	40-41	-1.3817900536149352
2214	42-43	-3.9489412549142955
2214	44-45	-1.723994205353364
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2214	50-51	-1.218157443586577
2214	52-53	-1.1370372958677066
2214	54-55	-1.1082463566264593
2214	56-57	-1.0875690349903735
2214	58-59	-1.2489162440221477
2214	60-61	-1.382261045254502
2214	62-63	-1.3347142521283288
2214	64-65	-1.2682508534168662
2214	66-67	-1.069976021395334
2214	68-69	-0.9709356154626434
2214	70-71	-1.0373169537175073
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2214	74-75	-1.0269070588738884
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2214	78-79	-1.064546487307446
2214	80-81	-1.0195126215566077
2214	82-83	-1.0745853866104653
2214	84-85	-1.1404808070239731
2214	86-87	-1.130238930191151
2214	88-89	-0.9530477434009086
2214	90-91	-0.9554888525757192
2214	92-93	-0.9966238317935705
2214	94-95	-1.123756260414165
2214	96-97	-1.0827816275932385
2214	98-99	-1.1950874686118418
2214	100	-1.013697770258144
2215	1	-4.038140764965636
2215	2	-2.1038057516227937
2215	3	-3.7167185795826043
2215	4	-2.039249112522654
2215	5	-1.4966512601490862
2215	6	-1.2899952816314624
2215	7	-1.286568000978633
2215	8	-1.2414997662162648
2215	9	-1.7235840469958745
2215	10-11	-1.3608712552065612
2215	12-13	-1.3075422252830933
2215	14-15	-3.5592826318056225
2215	16-17	-3.3691709019326552
2215	18-19	-2.231198818161694
2215	20-21	-1.7197355409623256
2215	22-23	-3.1537635988564503
2215	24-25	-5.383739078493214
2215	26-27	-3.5487579404448297
2215	28-29	-1.7223394462441064
2215	30-31	-3.8678276215636913
2215	32-33	-4.0595736739835
2215	34-35	-2.9666377337281986
2215	36-37	-1.9248261242100924
2215	38-39	-1.6618935424965073
2215	40-41	-1.913902447699165
2215	42-43	-1.762776658945775
2215	44-45	-3.2990659216429705
2215	46-47	-2.6854877998577535
2215	48-49	-1.859847404307196
2215	50-51	-5.026518925753635
2215	52-53	-5.432119329019329
2215	54-55	-3.134921429932298
2215	56-57	-1.8776993890395275
2215	58-59	-1.5715460127027328
2215	60-61	-3.256197187969992
2215	62-63	-2.8154338560183305
2215	64-65	-1.8172868958110868
2215	66-67	-1.5285743877816103
2215	68-69	-1.6973274035500978
2215	70-71	-1.4240068747902015
2215	72-73	-1.3632172510027765
2215	74-75	-1.507465302840032
2215	76-77	-1.6464459705291112
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2215	80-81	-1.487491080545837
2215	82-83	-1.4986703801928343
2215	84-85	-1.3059391402536384
2215	86-87	-1.3147907838951731
2215	88-89	-1.220049086085762
2215	90-91	-1.5756028356557366
2215	92-93	-3.8144872351029804
2215	94-95	-1.9572932080218095
2215	96-97	-1.5829070498948603
2215	98-99	-3.130551806188855
2215	100	-3.0646505180902075
2216	1	-0.662757117279348
2216	2	-0.7136285402259972
2216	3	-0.8656238753927354
2216	4	-0.8115988156802842
2216	5	-0.8934926143870143
2216	6	-0.8203377070052014
2216	7	-0.9460339035920526
2216	8	-0.7520326787612959
2216	9	-0.984839810510131
2216	10-11	-1.0475230800115511
2216	12-13	-1.26085471547578
2216	14-15	-1.5839062442282312
2216	16-17	-1.2665555311979944
2216	18-19	-3.1622560174408463
2216	20-21	-1.4633897279488224
2216	22-23	-0.9835186696032068
2216	24-25	-1.2692028966898121
2216	26-27	-1.1443193934901217
2216	28-29	-1.048920518641829
2216	30-31	-1.1922885931604768
2216	32-33	-1.2886574184404083
2216	34-35	-1.4548038076144323
2216	36-37	-1.4500181882585395
2216	38-39	-1.514762760138339
2216	40-41	-1.6220903091053813
2216	42-43	-1.5249498782589157
2216	44-45	-1.5253126488136317
2216	46-47	-1.6901777120263617
2216	48-49	-1.6457690923436559
2216	50-51	-1.4802988785839943
2216	52-53	-1.474988036963488
2216	54-55	-1.4018393590512304
2216	56-57	-1.4727896286477815
2216	58-59	-1.4712176533403962
2216	60-61	-1.48185460290469
2216	62-63	-1.5756180983655952
2216	64-65	-1.5708990183535079
2216	66-67	-1.5693678760650869
2216	68-69	-1.7616598809818456
2216	70-71	-1.7766820014105065
2216	72-73	-1.7203262848903762
2216	74-75	-1.7375463488793983
2216	76-77	-1.5338090034110863
2216	78-79	-1.6156165975283656
2216	80-81	-1.6327020445125768
2216	82-83	-1.7752785508313949
2216	84-85	-1.566025296628677
2216	86-87	-1.5523544691285025
2216	88-89	-1.4959677519027963
2216	90-91	-1.5260150587812298
2216	92-93	-1.4461403952953056
2216	94-95	-1.3884595401480055
2216	96-97	-1.419283360503794
2216	98-99	-1.3431565421228164
2216	100	-1.3623064534630096
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16040.0
1	2729.0
2	445.0
3	382.0
4	410.0
5	236.0
6	214.0
7	248.0
8	471.0
9	813.0
10	1446.0
11	3007.0
12	5082.0
13	7213.0
14	9452.0
15	11857.0
16	14272.0
17	16742.0
18	19149.0
19	21753.0
20	24477.0
21	27936.0
22	31871.0
23	36859.0
24	41272.0
25	46272.0
26	52352.0
27	59621.0
28	70350.0
29	84092.0
30	100419.0
31	119069.0
32	146052.0
33	184295.0
34	248078.0
35	334580.0
36	493259.0
37	757569.0
38	703203.0
39	224802.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49386755211672	19.838545585392755	12.349414593068527	24.318172269421996
2	15.526599377003258	19.666948977018023	40.48796968273922	24.318481963239503
3	19.016861978148327	25.54602895145159	28.85188337262128	26.585225697778807
4	12.297170839262137	16.307426066223126	39.14238100731967	32.25302208719506
5	13.364874978214736	38.033969633905045	35.059025250402385	13.542130137477832
6	31.047280603072135	37.518838311194926	17.964331515299005	13.469549570433937
7	27.65298085330063	31.52417760344252	22.953495010645035	17.869346532611814
8	26.019522509518104	35.38430484210354	20.00426275826621	18.591909890112145
9	26.051652546470695	15.31853361335161	20.866337960714844	37.76347587946285
10-11	25.45965023365262	25.91616726831357	27.858301668888664	20.765880829145154
12-13	25.699172289197737	23.4949237517314	27.84609448417708	22.95980947489378
14-15	22.96928012437259	24.964988141248064	25.029637259441785	27.03609447493756
16-17	22.479091551533422	27.73312613775094	26.23301062287851	23.554771687837125
18-19	22.042106057123153	27.95337860303595	27.623925554360586	22.38058978548031
20-21	23.423821654367856	27.17100970158185	26.882980561560615	22.522188082489677
22-23	23.576282937604017	26.903353279761316	26.65487670224984	22.865487080384828
24-25	23.171878948333827	26.950658093050162	27.160662954511345	22.716800004104666
26-27	23.085716850647163	27.14303027582647	26.495987483127553	23.275265390398815
28-29	22.514085611636368	27.584700597308682	26.87690270061809	23.024311090436868
30-31	23.073559787888833	27.276164556093452	26.817864382497813	22.832411273519902
32-33	23.25151363764916	27.379061297755502	26.231819834633363	23.137605229961977
34-35	23.343903775919784	27.313791034651818	26.76518929755195	22.577115891876442
36-37	23.163637035461324	26.939015719047845	26.573758717657842	23.323588527832996
38-39	23.2148898041957	27.087217461170056	27.009758335996338	22.68813439863791
40-41	23.045766982015834	26.488765637777036	27.381161618351648	23.084305761855486
42-43	22.880617299693572	26.631094209069374	26.962448405910365	23.52584008532669
44-45	23.06394882923551	27.244500931340966	26.88274187600989	22.808808363413632
46-47	23.072354612773516	27.05724755354501	26.5153086825561	23.355089151125377
48-49	23.43812228231975	26.859341893778936	26.68220888073869	23.02032694316262
50-51	23.186497157616376	26.814246888284586	26.730118387965547	23.269137566133494
52-53	22.85137865951693	27.16818288983987	26.58445342978738	23.395985020855818
54-55	22.762797110719244	27.064480283786423	26.674622887695154	23.498099717799178
56-57	23.476098354120293	26.7605463980973	26.449992650146726	23.31336259763568
58-59	22.7754246936027	26.955791791837502	27.019609196325295	23.249174318234502
60-61	22.759289281358175	27.36257279111926	26.65974783061653	23.218390096906035
62-63	23.00936090046673	26.92918265818893	26.438544392354647	23.62291204898969
64-65	22.533282056820976	27.072574651324423	26.619180306124886	23.77496298572972
66-67	22.920875426274204	26.803467789159342	26.614467251045284	23.66118953352117
68-69	22.589146530941637	26.864164204362478	26.807853028986976	23.738836235708906
70-71	22.572385610518516	26.60051886531053	26.926913469768092	23.900182054402862
72-73	22.938012309868792	26.625533709230886	27.174691531683116	23.261762449217205
74-75	23.041552449735892	26.873148043742823	26.853713687418324	23.23158581910296
76-77	22.90769534066316	26.75491033195333	26.638946062954705	23.698448264428805
78-79	22.7674100229281	26.75065082698972	26.728569540655357	23.753369609426823
80-81	22.494027718773282	26.984603417950915	27.156114097251727	23.365254766024083
82-83	23.058675128068405	26.351210340546448	27.791571837405943	22.798542693979204
84-85	23.240027801289028	26.701950220914544	26.843586448385327	23.2144355294111
86-87	22.354045089314035	27.109203404789966	27.236595431361625	23.30015607453437
88-89	22.981015760610177	26.23544389609086	27.113322876064593	23.670217467234373
90-91	22.795368339569087	26.31324990323829	27.23052509353632	23.6608566636563
92-93	23.007186169526513	26.404889691652688	27.22379047864792	23.364133660172882
94-95	22.269808109343924	26.39315805716775	27.66490716150255	23.67212667198578
96-97	22.555341996288043	26.278420562790167	27.352375113628064	23.813862327293727
98-99	22.123388738280706	26.76345095048283	27.235783265609104	23.877377045627355
100	22.701906093213267	26.268239055534657	26.775555318190474	24.2542995330616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13474.0
1	7933.5
2	2299.0
3	1745.0
4	957.0
5	542.0
6	439.5
7	427.5
8	443.0
9	442.5
10	403.5
11	370.5
12	374.0
13	385.5
14	427.5
15	516.5
16	604.5
17	699.5
18	882.0
19	1139.5
20	1478.5
21	1981.5
22	2791.0
23	3972.0
24	5528.0
25	7489.0
26	10199.0
27	13652.0
28	18355.0
29	25806.0
30	35510.5
31	44749.0
32	56126.5
33	67243.0
34	76232.0
35	85963.0
36	95096.5
37	109634.0
38	117821.0
39	114706.0
40	111202.0
41	107743.0
42	108656.0
43	115142.5
44	124233.0
45	139838.0
46	155202.5
47	165435.5
48	172393.5
49	171569.0
50	166203.0
51	168022.5
52	168652.0
53	162592.5
54	155420.5
55	139273.5
56	123851.0
57	106217.0
58	88850.5
59	81436.0
60	68171.5
61	51530.5
62	39779.0
63	31697.0
64	23458.5
65	14477.0
66	9610.0
67	6971.0
68	5117.5
69	3721.0
70	2746.0
71	2092.5
72	1503.5
73	1094.5
74	846.5
75	585.5
76	388.5
77	266.0
78	177.5
79	106.5
80	66.0
81	47.5
82	42.5
83	29.0
84	18.0
85	17.5
86	20.0
87	16.5
88	10.0
89	11.0
90	8.5
91	6.5
92	6.0
93	3.5
94	4.5
95	5.5
96	3.0
97	2.5
98	3.0
99	2.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9978281123563335
2	0.44828516315696665
3	0.4815869299879476
4	0.46599506827627757
5	0.4335864522798441
6	0.4868437606141407
7	0.5029715128265391
8	0.5059571884734546
9	0.5204262319931222
10-11	0.5342955885238794
12-13	0.5321137486280565
14-15	0.5294087775291416
16-17	0.5344742186907889
18-19	0.5330068994626039
20-21	0.5318458033776923
22-23	0.4937720592307011
24-25	0.5283880337182303
26-27	0.5096956626809173
28-29	0.5139572680914719
30-31	0.5176064272154798
32-33	0.5206686586482137
34-35	0.5331472517366042
36-37	0.5509975091299154
38-39	0.5402669398177105
40-41	0.5445157859306288
42-43	0.5415045916884403
44-45	0.5522734388935546
46-47	0.5510102684275519
48-49	0.5233991483424014
50-51	0.5384933974462521
52-53	0.529715000672415
54-55	0.5392589553044356
56-57	0.5275714386695013
58-59	0.5326113612358758
60-61	0.5271248632522276
62-63	0.5211279933631238
64-65	0.5330834552484223
66-67	0.5317692475918739
68-69	0.5516099554164622
70-71	0.5352525358466087
72-73	0.5386082311249796
74-75	0.5352397765489724
76-77	0.5238329644620386
78-79	0.554927372801924
80-81	0.528311477932412
82-83	0.528145607063139
84-85	0.5371664304920675
86-87	0.8438361491827288
88-89	1.111985548309125
90-91	1.102684020332196
92-93	1.102684020332196
94-95	1.1069711443380235
96-97	1.100565976924555
98-99	1.095870555394363
100	1.092833842556902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3918711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.143868013970426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05951767913696	34.787646953271796
2	12.381687291941876	11.179145137953581
3	4.463699986503219	6.045260491339886
4	2.0837569367187188	3.7627539249770066
5	1.122716509550206	2.5341882962120033
6	0.6539784246156733	1.771386941090056
7	0.41541474807585904	1.3127399990735347
8	0.29258260690159127	1.0566648471319062
9	0.213096807142425	0.8658012722252528
>10	1.0640550494250656	8.873823934721248
>50	0.10436812079494637	3.3215826401491033
>100	0.11797694303674049	11.493732471970945
>500	0.01896471748975748	5.780860773689903
>1k	0.007789080386662585	5.891980696016776
>5k	3.386556689853298E-4	1.0156988293545155
>10k+	5.644261149755497E-5	0.3067327908225793
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12038	0.30719284989375334	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8868	0.22629890287903343	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7490	0.19113427859313942	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6607	0.16860135896727266	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5822	0.14856926167813855	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5712	0.1457622161981325	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5363	0.13685622644793147	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4583	0.11695172213516128	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4060	0.10360549680749613	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.10371905455646E-5
2	0.0	0.0	0.0	0.0	5.10371905455646E-5
3	0.0	0.0	0.0	0.0	5.10371905455646E-5
4	0.0	0.0	0.0	0.0	5.10371905455646E-5
5	0.0	0.0	0.0	0.0	5.10371905455646E-5
6	0.0	0.0	0.0	0.0	1.275929763639115E-4
7	0.0	0.0	0.0	0.0	1.275929763639115E-4
8	0.0	0.0	0.0	0.0	1.275929763639115E-4
9	0.0	0.0	0.0	0.0	1.275929763639115E-4
10-11	0.0	0.0	0.0	0.0	1.275929763639115E-4
12-13	0.0	1.275929763639115E-5	0.0	0.0	2.679452503642142E-4
14-15	0.0	2.55185952727823E-5	0.0	0.0	3.062231432733876E-4
16-17	0.0	2.55185952727823E-5	0.0	1.275929763639115E-5	3.572603338189522E-4
18-19	0.0	2.55185952727823E-5	0.0	2.55185952727823E-5	3.827789290917345E-4
20-21	0.0	2.55185952727823E-5	0.0	7.65557858183469E-5	4.4657541727369026E-4
22-23	0.0	2.55185952727823E-5	0.0	2.041487621822584E-4	4.720940125464726E-4
24-25	0.0	2.55185952727823E-5	0.0	2.679452503642142E-4	4.848533101828637E-4
26-27	0.0	2.55185952727823E-5	0.0	5.10371905455646E-4	5.358905007284284E-4
28-29	0.0	3.827789290917345E-5	0.0	0.0018245795620039345	5.869276912739929E-4
30-31	0.0	5.10371905455646E-5	0.0	0.006252055841831664	5.869276912739929E-4
32-33	0.0	5.10371905455646E-5	0.0	0.017250570404400836	6.124462865467752E-4
34-35	0.0	5.10371905455646E-5	0.0	0.03383765733170933	6.124462865467752E-4
36-37	0.0	5.10371905455646E-5	0.0	0.06425582289686582	6.124462865467752E-4
38-39	0.0	5.10371905455646E-5	0.0	0.11436158471497387	6.124462865467752E-4
40-41	0.0	5.10371905455646E-5	0.0	0.16892034140818243	6.124462865467752E-4
42-43	0.0	5.10371905455646E-5	0.0	0.22693686776085298	6.124462865467752E-4
44-45	0.0	5.10371905455646E-5	0.0	0.3004559407417388	6.252055841831663E-4
46-47	0.0	5.10371905455646E-5	0.0	0.3947088723817602	6.379648818195575E-4
48-49	0.0	5.10371905455646E-5	0.0	0.5044133134594513	6.634834770923398E-4
50-51	0.0	5.10371905455646E-5	0.0	0.6232661709424349	6.890020723651221E-4
52-53	0.0	5.10371905455646E-5	0.0	0.7443136276188778	7.145206676379044E-4
54-55	0.0	5.10371905455646E-5	0.0	0.880021517279534	7.145206676379044E-4
56-57	0.0	5.10371905455646E-5	0.0	1.0485590797586246	7.145206676379044E-4
58-59	0.0	5.10371905455646E-5	0.0	1.2440442788457735	7.272799652742956E-4
60-61	0.0	5.10371905455646E-5	0.0	1.4532202042967701	7.400392629106867E-4
62-63	0.0	5.10371905455646E-5	0.0	1.6726546050474251	7.910764534562513E-4
64-65	0.0	5.10371905455646E-5	0.0	1.8999614924397334	7.910764534562513E-4
66-67	0.0	5.10371905455646E-5	0.0	2.1638875640484843	7.910764534562513E-4
68-69	0.0	5.10371905455646E-5	0.0	2.466155325054591	7.910764534562513E-4
70-71	0.0	5.10371905455646E-5	0.0	2.8019417609514967	8.038357510926424E-4
72-73	0.0	5.10371905455646E-5	0.0	3.150704402544612	9.186694298201629E-4
74-75	0.0	5.10371905455646E-5	0.0	3.4927556535809865	0.001020743810911292
76-77	0.0	5.10371905455646E-5	0.0	3.8797196322974568	0.001020743810911292
78-79	0.0	5.10371905455646E-5	0.0	4.30641606385365	0.0010462624061840743
80-81	0.0	5.10371905455646E-5	0.0	4.778203853256849	0.0010590217038204653
82-83	0.0	5.10371905455646E-5	0.0	5.264205500226987	0.0011483367872752036
84-85	0.0	5.10371905455646E-5	0.0	5.746468672989664	0.0011610960849115946
86-87	0.0	5.10371905455646E-5	0.0	6.257529580517675	0.0012248925730935505
88	0.0	5.10371905455646E-5	0.0	6.668774502636198	0.001275929763639115
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6520	0.0	31.541912	1
GGTATCA	4350	0.0	31.22859	1
CAACGCA	6185	0.0	30.68969	5
GGGCACC	3270	0.0	29.153688	94
GTACTAG	2775	0.0	29.0658	1
ATCAACG	6570	0.0	28.825321	3
GTACATG	13650	0.0	27.126022	1
TAGTACT	3985	0.0	26.649015	4
AACGCAG	7610	0.0	26.528763	6
CTAGTAC	4285	0.0	26.21088	3
TACATGG	13810	0.0	26.203846	2
GCATCGT	2950	0.0	26.10288	8
CTGTGCG	3945	0.0	26.067364	9
ACATGGG	13885	0.0	25.41725	3
TATCAAC	8010	0.0	25.346567	2
TAGGCAT	3805	0.0	24.695976	5
GGCGTCG	2035	0.0	24.688004	8
ACGCAGA	7785	0.0	24.064413	7
TCTAACG	1345	0.0	23.760447	2
CTAACGC	1350	0.0	23.67062	3
>>END_MODULE