##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3918711
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80463040014944	31.0	31.0	34.0	30.0	34.0
2	31.766972098733486	31.0	31.0	34.0	30.0	34.0
3	31.974461245036952	31.0	31.0	34.0	30.0	34.0
4	35.72375686801094	37.0	35.0	37.0	35.0	37.0
5	35.613657909450325	37.0	35.0	37.0	33.0	37.0
6	35.65936528618722	37.0	35.0	37.0	33.0	37.0
7	35.58178059060747	37.0	35.0	37.0	33.0	37.0
8	35.56967788642745	37.0	35.0	37.0	33.0	37.0
9	37.166707368826124	39.0	37.0	39.0	33.0	39.0
10-11	37.11054693750062	39.0	37.0	39.0	33.0	39.0
12-13	37.0480965552193	39.0	37.0	39.0	33.0	39.0
14-15	38.26258557979907	40.0	38.0	41.0	33.0	41.0
16-17	38.25537109013653	40.0	38.0	41.0	33.0	41.0
18-19	38.30859024817089	40.0	38.0	41.0	33.0	41.0
20-21	38.3240005450772	40.0	38.0	41.0	33.0	41.0
22-23	38.29436988336215	40.0	38.0	41.0	33.0	41.0
24-25	38.23979926052215	40.0	37.5	41.0	33.0	41.0
26-27	38.11007777812653	40.0	37.0	41.0	33.0	41.0
28-29	38.037225123261194	40.0	37.0	41.0	33.0	41.0
30-31	37.917737235534844	40.0	37.0	41.0	33.0	41.0
32-33	37.8094933512576	40.0	37.0	41.0	33.0	41.0
34-35	37.71994171042468	40.0	37.0	41.0	32.5	41.0
36-37	37.63766223127962	40.0	36.0	41.0	32.0	41.0
38-39	37.537804650559835	40.0	36.0	41.0	32.0	41.0
40-41	37.42085420435444	40.0	36.0	41.0	31.0	41.0
42-43	37.30430044471256	40.0	36.0	41.0	31.0	41.0
44-45	37.14657740772412	39.0	36.0	41.0	31.0	41.0
46-47	36.94415765796457	39.0	35.0	41.0	30.5	41.0
48-49	36.727483220885645	39.0	35.0	41.0	30.0	41.0
50-51	36.5543288341498	39.0	35.0	41.0	30.0	41.0
52-53	36.38247704410966	39.0	35.0	41.0	30.0	41.0
54-55	36.19349398309801	38.5	35.0	40.5	29.0	41.0
56-57	35.974613846236686	38.0	35.0	40.0	29.0	41.0
58-59	35.72990531835596	38.0	34.0	40.0	28.5	41.0
60-61	35.481558476754216	37.0	34.0	40.0	28.0	41.0
62-63	35.21824127882867	37.0	34.0	40.0	28.0	41.0
64-65	34.947476096093844	36.0	34.0	39.5	28.0	41.0
66-67	34.71748529044372	36.0	34.0	39.0	28.0	41.0
68-69	34.51777829495464	35.5	33.5	39.0	28.0	41.0
70-71	34.266574901798066	35.0	33.0	38.5	27.5	40.5
72-73	34.03466586334129	35.0	33.0	37.5	27.0	40.0
74-75	33.83397882620076	35.0	33.0	37.0	27.0	39.5
76-77	33.64588674694306	35.0	33.0	37.0	27.0	39.0
78-79	33.49663894581663	35.0	33.0	36.0	27.0	39.0
80-81	33.31294257218764	35.0	33.0	36.0	27.0	38.5
82-83	33.10666275211415	35.0	33.0	35.5	27.0	37.0
84-85	32.94296517400747	35.0	33.0	35.0	27.0	37.0
86-87	32.79058254104474	35.0	33.0	35.0	27.0	36.5
88-89	32.663310461016394	35.0	33.0	35.0	27.0	36.0
90-91	32.56529787473483	35.0	33.0	35.0	27.0	36.0
92-93	32.48397866032989	35.0	33.0	35.0	27.0	36.0
94-95	32.42807520125878	35.0	33.0	35.0	27.0	35.0
96-97	32.37299879986046	35.0	33.0	35.0	26.0	35.0
98-99	32.32318637429502	35.0	33.0	35.0	26.0	35.0
100	31.830721377514188	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43919961130655594
1101	2	0.5358167676456205
1101	3	0.4558080374656761
1101	4	0.30740723446700713
1101	5	0.3407830739930233
1101	6	0.3873078840036257
1101	7	0.3823263315722656
1101	8	0.3697171840519786
1101	9	0.40818510423842014
1101	10-11	0.48158238208327475
1101	12-13	0.5339828592382361
1101	14-15	0.7038917361768213
1101	16-17	0.6760825731332005
1101	18-19	0.6202927692416793
1101	20-21	0.6507161918398552
1101	22-23	0.6601965701833592
1101	24-25	0.6809419286453817
1101	26-27	0.7100118029272977
1101	28-29	0.6601721006372188
1101	30-31	0.7553823653798588
1101	32-33	0.7249873142301411
1101	34-35	0.7334113403524825
1101	36-37	0.6942702176329547
1101	38-39	0.7132838811236795
1101	40-41	0.693771884967262
1101	42-43	0.6148163861282896
1101	44-45	0.5946048287898051
1101	46-47	0.6666156262677418
1101	48-49	0.683066455833
1101	50-51	0.632046687071167
1101	52-53	0.6320742345988393
1101	54-55	0.562000181572138
1101	56-57	0.5196635949520001
1101	58-59	0.5067989958537638
1101	60-61	0.6048388881266717
1101	62-63	0.5802592947522456
1101	64-65	0.5792373793347281
1101	66-67	0.5543143557526378
1101	68-69	0.5280063711452172
1101	70-71	0.44476068958502424
1101	72-73	0.4506410143252353
1101	74-75	0.38952155165423363
1101	76-77	0.45155593469109334
1101	78-79	0.46318170917432866
1101	80-81	0.476935567208713
1101	82-83	0.4559812432154189
1101	84-85	0.4941889564256954
1101	86-87	0.48203222237677323
1101	88-89	0.4445586522940488
1101	90-91	0.4501471758733473
1101	92-93	0.4682494717057821
1101	94-95	0.3846856127121825
1101	96-97	0.49075634558721504
1101	98-99	0.5045912610229735
1101	100	0.45128242483151126
1102	1	0.3879677818918097
1102	2	0.5338082459826623
1102	3	0.40260893658998853
1102	4	0.2959025748067816
1102	5	0.29468364241375866
1102	6	0.29822541669189917
1102	7	0.33229476920514145
1102	8	0.3329409214888628
1102	9	0.35809540673113816
1102	10-11	0.37626298694566174
1102	12-13	0.418952049917678
1102	14-15	0.5481460830794731
1102	16-17	0.5400448545122174
1102	18-19	0.6047091774012117
1102	20-21	0.5666406100866581
1102	22-23	0.5534265203559912
1102	24-25	0.5330544390321279
1102	26-27	0.6171787653423735
1102	28-29	0.6453761112084351
1102	30-31	0.6275681536878892
1102	32-33	0.6477553903356252
1102	34-35	0.6438693615098288
1102	36-37	0.6255887803404008
1102	38-39	0.6668275079146184
1102	40-41	0.6009897054664961
1102	42-43	0.6068876744997524
1102	44-45	0.5927108970441495
1102	46-47	0.6365959137122701
1102	48-49	0.6700931859910071
1102	50-51	0.6066768777904699
1102	52-53	0.5576488015347323
1102	54-55	0.5975638047372911
1102	56-57	0.6417901572390363
1102	58-59	0.5841365251439186
1102	60-61	0.569545925370484
1102	62-63	0.543847183118352
1102	64-65	0.5460056393044468
1102	66-67	0.5175725948367997
1102	68-69	0.5547088031644378
1102	70-71	0.5244325683493258
1102	72-73	0.5510282323023787
1102	74-75	0.5772893815323314
1102	76-77	0.5105930910138312
1102	78-79	0.49588288813057346
1102	80-81	0.48920210888528004
1102	82-83	0.4668021170860399
1102	84-85	0.46377443986709466
1102	86-87	0.5120831707126854
1102	88-89	0.45432290305311085
1102	90-91	0.5162641081624955
1102	92-93	0.5758945570274321
1102	94-95	0.494441816700558
1102	96-97	0.5022260022015033
1102	98-99	0.5048693882063446
1102	100	0.518012594765807
1103	1	0.2733904412347812
1103	2	-0.16735093593803185
1103	3	0.1759004847604011
1103	4	0.20077541720711878
1103	5	0.20698555159742682
1103	6	0.26992116459107507
1103	7	0.30800415592882757
1103	8	0.32596584879564716
1103	9	0.37613889739027684
1103	10-11	0.4030800151917333
1103	12-13	0.41923406969451094
1103	14-15	0.5053755763601657
1103	16-17	0.5453264911924407
1103	18-19	0.5042042696529805
1103	20-21	0.5094484258108878
1103	22-23	0.4988047293156441
1103	24-25	0.45288136878843943
1103	26-27	0.4976339564929404
1103	28-29	0.49213431689413767
1103	30-31	0.5616526508795232
1103	32-33	0.5538708682393931
1103	34-35	0.5215140420864373
1103	36-37	0.5542314295602537
1103	38-39	0.5556997665284342
1103	40-41	0.5856667462489753
1103	42-43	0.5455876806926412
1103	44-45	0.54553242414039
1103	46-47	0.4990759036013941
1103	48-49	0.5650797408837747
1103	50-51	0.5857377809419688
1103	52-53	0.5612937015635566
1103	54-55	0.47044734903899865
1103	56-57	0.5096190086792305
1103	58-59	0.5276384922165533
1103	60-61	0.5668674028198524
1103	62-63	0.5815509678227002
1103	64-65	0.49783743586464624
1103	66-67	0.5058450484559671
1103	68-69	0.4419900332486364
1103	70-71	0.44041135328719605
1103	72-73	0.3695794570285571
1103	74-75	0.4099540101445953
1103	76-77	0.4744105684103985
1103	78-79	0.4429161036837712
1103	80-81	0.42021947799817383
1103	82-83	0.36716860440743204
1103	84-85	0.399306214543266
1103	86-87	0.3874675813396493
1103	88-89	0.40163599602284705
1103	90-91	0.46105906285875875
1103	92-93	0.4668896684806043
1103	94-95	0.4812463410141987
1103	96-97	0.46814554755060556
1103	98-99	0.4640490015377168
1103	100	0.47390424392256136
1104	1	0.2965685918509777
1104	2	0.3852123281305495
1104	3	0.34220490754996646
1104	4	0.2399199132809855
1104	5	0.24468435375001718
1104	6	0.23564013985384236
1104	7	0.3073399927681777
1104	8	0.26598235455973196
1104	9	0.3594790933095169
1104	10-11	0.2608842454512086
1104	12-13	0.3720838853122075
1104	14-15	0.41017124090769386
1104	16-17	0.4029458336488858
1104	18-19	0.43542775828772307
1104	20-21	0.41017199454378783
1104	22-23	0.4037280706000601
1104	24-25	0.44579333961228684
1104	26-27	0.47466458911809895
1104	28-29	0.4572793239933759
1104	30-31	0.4964367986483893
1104	32-33	0.49465586244544824
1104	34-35	0.5471317696835882
1104	36-37	0.550218747278592
1104	38-39	0.5092842375132776
1104	40-41	0.5511892110524244
1104	42-43	0.5449977910821318
1104	44-45	0.5778359388236893
1104	46-47	0.6095901963241488
1104	48-49	0.5765522712000006
1104	50-51	0.6896749769899415
1104	52-53	0.6309463701559608
1104	54-55	0.6675094599569746
1104	56-57	0.6799242522724569
1104	58-59	0.6680642216251442
1104	60-61	0.645436373612668
1104	62-63	0.6555707566422129
1104	64-65	0.5377585403184355
1104	66-67	0.49877757121427635
1104	68-69	0.5915815273960376
1104	70-71	0.563488649497522
1104	72-73	0.5370984474007727
1104	74-75	0.5019604566323324
1104	76-77	0.5103651097072728
1104	78-79	0.4186099716207323
1104	80-81	0.47811210147769145
1104	82-83	0.4560674896062906
1104	84-85	0.462245525410502
1104	86-87	0.4693272764009535
1104	88-89	0.4060423626167591
1104	90-91	0.41275006249397705
1104	92-93	0.39977274240889216
1104	94-95	0.4047764007193635
1104	96-97	0.4847234490545347
1104	98-99	0.44725367940743865
1104	100	0.4975974798842131
1105	1	0.09350666844705202
1105	2	-0.4673857841102276
1105	3	-0.034429033804915576
1105	4	0.0524539831439057
1105	5	0.083508906239949
1105	6	0.1763593130563379
1105	7	0.2244527259830349
1105	8	0.20021403542411065
1105	9	0.2298627018686119
1105	10-11	0.28085569949276135
1105	12-13	0.28098856356827895
1105	14-15	0.3803596002875125
1105	16-17	0.38409365186333133
1105	18-19	0.3736822139093192
1105	20-21	0.36453154178245484
1105	22-23	0.3022641600868283
1105	24-25	0.38288197537941926
1105	26-27	0.356201341972195
1105	28-29	0.3460372094841233
1105	30-31	0.3938445570937148
1105	32-33	0.4225719926509228
1105	34-35	0.3955762805112002
1105	36-37	0.37713826280072027
1105	38-39	0.35147505894156694
1105	40-41	0.3783794251915964
1105	42-43	0.35803966518307817
1105	44-45	0.2865896642829071
1105	46-47	0.3523282924127926
1105	48-49	0.3852664723155499
1105	50-51	0.4301815787820402
1105	52-53	0.41292988828896426
1105	54-55	0.35589801121588494
1105	56-57	0.3552939808041131
1105	58-59	0.3337579271038038
1105	60-61	0.4150565233711063
1105	62-63	0.3094141148741798
1105	64-65	0.3765710683491079
1105	66-67	0.4564249453190712
1105	68-69	0.4008686697307198
1105	70-71	0.3885382537620501
1105	72-73	0.36650356154349595
1105	74-75	0.33535119372763944
1105	76-77	0.29368483913537347
1105	78-79	0.2991933412274008
1105	80-81	0.3226078580702847
1105	82-83	0.3170405972806094
1105	84-85	0.32217435193516053
1105	86-87	0.2670395691054068
1105	88-89	0.34029117698754874
1105	90-91	0.2862280011835239
1105	92-93	0.23790758701365178
1105	94-95	0.23964826271631523
1105	96-97	0.24177899599016683
1105	98-99	0.25911366509402
1105	100	0.3555405990693927
1106	1	0.26971692626248256
1106	2	0.2953393485442817
1106	3	0.26526195568873234
1106	4	0.22000447986079052
1106	5	0.20689856815512542
1106	6	0.22208633444173387
1106	7	0.2364782015158724
1106	8	0.2487287515911305
1106	9	0.2714290484577333
1106	10-11	0.29372500371577814
1106	12-13	0.26012172076229234
1106	14-15	0.32536493046873005
1106	16-17	0.31942205302897264
1106	18-19	0.3373888466014989
1106	20-21	0.3351002077837677
1106	22-23	0.27939818264360383
1106	24-25	0.28879597645545374
1106	26-27	0.30315240497499474
1106	28-29	0.27926087242200026
1106	30-31	0.3119217050500822
1106	32-33	0.22652346033415682
1106	34-35	0.2750237150573014
1106	36-37	0.3402167408023118
1106	38-39	0.23640794665975307
1106	40-41	0.26678643102980004
1106	42-43	0.23615765452804993
1106	44-45	0.2396094002872644
1106	46-47	0.24705637983442585
1106	48-49	0.20389337229681814
1106	50-51	0.2920023473548383
1106	52-53	0.2552091257830327
1106	54-55	0.253908276898251
1106	56-57	0.21327488548820384
1106	58-59	0.27623543800584116
1106	60-61	0.2963062191941077
1106	62-63	0.2235798631762833
1106	64-65	0.2184886935483661
1106	66-67	0.16330051242168508
1106	68-69	0.16829182878478832
1106	70-71	0.21889955271287675
1106	72-73	0.08523386504048602
1106	74-75	0.13114705078677957
1106	76-77	0.09358176896432013
1106	78-79	0.13578405197822718
1106	80-81	0.16748798399456177
1106	82-83	0.21376275250054988
1106	84-85	0.15855632225549243
1106	86-87	0.15981705687332948
1106	88-89	0.1644640655324281
1106	90-91	0.1801511279031658
1106	92-93	0.14718643582784807
1106	94-95	0.20833866485665453
1106	96-97	0.1547547038308963
1106	98-99	0.16258071630710447
1106	100	0.21263454026338735
1107	1	0.04248190173050048
1107	2	-0.4328366243025421
1107	3	-0.04089919301410916
1107	4	0.04591247353191363
1107	5	0.08618192924495105
1107	6	0.15983219060569098
1107	7	0.1451496458632846
1107	8	0.11032492469393418
1107	9	0.16978511174912825
1107	10-11	0.24241312175973917
1107	12-13	0.1586158734170482
1107	14-15	0.21417177104087415
1107	16-17	0.2931635552854601
1107	18-19	0.24187067814700214
1107	20-21	0.24183219626399932
1107	22-23	0.19540295108318873
1107	24-25	0.21586776246182637
1107	26-27	0.2785037836573565
1107	28-29	0.1318523912795797
1107	30-31	0.26103273827139617
1107	32-33	0.3379458005792273
1107	34-35	0.35456543263287443
1107	36-37	0.4012272882685508
1107	38-39	0.37757257069957717
1107	40-41	0.3575378761165027
1107	42-43	0.3325602021843892
1107	44-45	0.3960467607209708
1107	46-47	0.32289390964542974
1107	48-49	0.3884810283486857
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2216	82-83	-0.6202861401992692
2216	84-85	-0.7176520304340883
2216	86-87	-0.6611321338818641
2216	88-89	-0.6472991386255273
2216	90-91	-0.6699116367911486
2216	92-93	-0.6212946585698091
2216	94-95	-0.649015193356437
2216	96-97	-0.6471528800496387
2216	98-99	-0.6683308894868034
2216	100	-0.677845142513334
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	45.0
14	172.0
15	606.0
16	1415.0
17	3017.0
18	5227.0
19	8291.0
20	11839.0
21	16883.0
22	23336.0
23	28160.0
24	31148.0
25	33519.0
26	38448.0
27	47340.0
28	59196.0
29	74479.0
30	92693.0
31	114071.0
32	142051.0
33	179236.0
34	239980.0
35	328016.0
36	488919.0
37	831428.0
38	826593.0
39	291743.0
40	854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65322348270327	20.624072111233588	13.527798315999132	25.194906090064006
2	14.97110512528343	20.25683198864876	39.43123861973222	25.340824266335588
3	18.2401873306329	25.31132667948236	29.86061721013425	26.58786877975049
4	12.471775027248617	16.870914302779997	37.8391017017942	32.81820896817719
5	12.859991175426549	38.16148254875526	35.180675358029376	13.797850917788814
6	30.787087897015113	38.15670510022301	17.346673434198134	13.709533568563744
7	26.812114747384015	32.10903119533416	22.924136734295473	18.154717322986354
8	26.081790670452605	35.52162943376023	19.54071121856141	18.85586867722575
9	25.77648619660904	14.919497763422719	20.803141645301224	38.50087439466702
10-11	25.405267191175874	26.062945187843656	27.566156320279806	20.965631300700664
12-13	25.757155860689906	23.555322145470793	28.06458552314779	22.62293647069151
14-15	22.903987564175495	24.8492161382947	25.64115520867547	26.60564108885433
16-17	22.03363424471855	27.613988226138673	26.731427114638397	23.620950414504378
18-19	21.622148201283533	27.18800901623008	29.072876259565966	22.11696652292042
20-21	22.79602216035379	26.37866798002404	28.655501427765767	22.169808431856403
22-23	22.913975763015127	26.120999147544687	28.423917183347815	22.54110790609237
24-25	22.571299593157036	26.219973863854722	28.942807979460593	22.265918563527652
26-27	22.48008238436963	26.42258430050033	28.226190568847436	22.871142746282604
28-29	21.97526176337066	26.828400971646033	28.616501701707524	22.579835563275783
30-31	22.592377952852356	26.52538041207938	28.45077118470844	22.431470450359825
32-33	22.801247655160076	26.614172364331022	28.00045729322729	22.584122687281607
34-35	22.832192860123076	26.537023167151307	28.467997636968427	22.162786335757183
36-37	22.687664387600922	26.21803445061399	28.229792908943786	22.864508252841304
38-39	22.747875513147054	26.306635013401092	28.661758930423805	22.283730543028053
40-41	22.510949441561255	25.817281425209888	28.972797818048008	22.698971315180852
42-43	22.57811386544956	25.934059792739227	28.349779958156894	23.138046383654316
44-45	22.72864725160901	26.54938065093343	28.143744205683962	22.578227891773597
46-47	22.80395262625899	26.435172177790093	27.594979573640416	23.1658956223105
48-49	23.3304025080821	26.400840688786044	27.496423232091633	22.77233357104023
50-51	22.955915861108412	26.413647242677502	27.385522943641416	23.24491395257267
52-53	22.61926179297223	26.73704440056947	27.253528009592948	23.390165796865347
54-55	22.585330724981333	26.68752650755583	27.226456059084992	23.50068670837784
56-57	23.242921708614602	26.404780964741686	27.021183262744575	23.33111406389914
58-59	22.51268691937878	26.58705951448458	27.61701803518334	23.283235530953302
60-61	22.573490073709216	26.97714643047667	27.222660929573884	23.226702566240238
62-63	22.865069661937305	26.51482847293408	26.919885135698955	23.700216729429652
64-65	22.29816130865481	26.61881674867067	27.217636105341782	23.865385837332738
66-67	22.636537881971904	26.428907362650627	27.182624592627526	23.751930162749947
68-69	22.406965032188292	26.298689782045447	27.427149222655387	23.86719596311087
70-71	22.317006183846463	26.133650957497778	27.482830623341677	24.066512235314082
72-73	22.743256647402678	26.08477634609952	27.648568623713253	23.523398382784542
74-75	22.778897550629974	26.468397758798417	27.246129872356956	23.506574818214656
76-77	22.63506245704794	26.405860951311087	26.94397226773861	24.01510432390236
78-79	22.4549587885132	26.42134907873526	27.114106273807387	24.009585858944153
80-81	22.189122269992936	26.764414099159072	27.403876764739472	23.642586866108516
82-83	22.807419581917713	26.09235547980347	28.030111540243457	23.07011339803536
84-85	22.957616002204947	26.425776963398885	27.06398687239375	23.55262016200242
86-87	22.08616105864166	26.807480315714187	27.395281385916636	23.71107723972752
88-89	22.60159636667842	26.073614778284394	27.26845256658461	24.056336288452577
90-91	22.350853947471844	26.094421652322776	27.40688547784004	24.14783892236535
92-93	22.611420062763525	26.33234044307074	27.19221582200812	23.864023672157614
94-95	21.789085673506246	26.228101928769394	27.648021117538658	24.33479128018571
96-97	22.011295879541922	26.147180744918636	27.354336477769536	24.487186897769906
98-99	21.611824716205714	26.638288620323458	27.21939591534256	24.530490748128273
100	22.118009370217585	26.133732987620117	26.805161383915344	24.943096258246953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	152.0
2	174.0
3	219.5
4	138.0
5	116.0
6	259.5
7	675.5
8	1145.0
9	1305.5
10	1102.5
11	802.0
12	763.5
13	853.5
14	978.5
15	1211.0
16	1529.5
17	1936.5
18	2503.5
19	3148.5
20	3787.5
21	4680.5
22	5845.5
23	7136.0
24	8759.0
25	10730.5
26	13245.0
27	16474.5
28	20600.0
29	27831.5
30	37293.0
31	45769.0
32	56660.0
33	67646.0
34	75913.0
35	84560.0
36	93531.0
37	108174.5
38	116370.0
39	113157.0
40	109399.5
41	106502.0
42	107734.0
43	113495.0
44	122328.0
45	138319.0
46	153621.0
47	164590.0
48	173009.0
49	171929.5
50	165860.0
51	167187.5
52	167543.5
53	161790.5
54	156660.5
55	140959.0
56	124641.5
57	106588.5
58	88769.5
59	82622.0
60	69184.0
61	51476.0
62	39325.5
63	30771.0
64	22242.0
65	13351.0
66	8846.0
67	6401.5
68	4658.0
69	3342.5
70	2450.0
71	1788.0
72	1265.5
73	990.0
74	719.5
75	453.5
76	278.5
77	161.0
78	91.0
79	37.0
80	16.5
81	12.0
82	8.5
83	6.5
84	4.0
85	0.5
86	1.5
87	1.5
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013831078637848007
2	0.005001644673465331
3	0.0019904504312770197
4	0.027585601489877664
5	0.0027560082894604885
6	0.0
7	4.0829752436451677E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009033582726564935
16-17	0.0029473977540063557
18-19	0.0
20-21	2.5518595272782298E-5
22-23	7.783171558198601E-4
24-25	0.0
26-27	1.6587086927308495E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.0829752436451677E-4
36-37	0.0
38-39	0.0
40-41	4.5933471491008143E-4
42-43	6.252055841831663E-4
44-45	0.0
46-47	0.0
48-49	4.976126078192549E-4
50-51	0.0
52-53	0.0
54-55	4.3381611963729905E-4
56-57	1.0207438109112919E-4
58-59	2.4242665509143185E-4
60-61	3.8277892909173446E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0012759297636391151
70-71	8.54872941638207E-4
72-73	0.0
74-75	2.4242665509143185E-4
76-77	0.0025008223367326654
78-79	5.614090960012106E-4
80-81	0.0027687675870968795
82-83	0.002054246919458975
84-85	0.006405167413468357
86-87	0.007489707712561604
88-89	0.00798732032038086
90-91	0.0051802748403748065
92-93	0.008995304833655762
94-95	0.0018500981572767167
96-97	0.005167515542738415
98-99	0.0018245795620039345
100	0.08490036647254671
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3918711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.4274461948475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79362540021934	27.204182310051184
2	15.652480749460928	12.029697236315922
3	5.69046019045583	6.560095583779891
4	2.6519020674932765	4.076232960504108
5	1.4255053729711986	2.7389265510158376
6	0.8550078807178769	1.9713461599474082
7	0.5547035286492543	1.4921088000882887
8	0.3883641381446026	1.1939073618048028
9	0.26631579137515426	0.9210452169516276
>10	1.392514429291863	9.801980279646964
>50	0.13756889956232493	3.686423530401746
>100	0.154712276393467	12.910169921665881
>500	0.02533531385774877	6.7005732882345725
>1k	0.010905489426509667	7.129762404888494
>5k	5.319750939760813E-4	1.3252836640185826
>10k+	6.649688674701017E-5	0.2582647306847874
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10107	0.2579164424220107	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7405	0.18896519799495293	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7169	0.18294280951057632	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7160	0.1827131421531213	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6540	0.16689161308399625	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6228	0.15892981135888817	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6194	0.15806217911961357	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6085	0.1552806522348803	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5083	0.12971101977155242	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4050	0.10335031085476831	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4017	0.1025081972107665	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.4035227400030265E-4
14-15	0.0	1.275929763639115E-5	0.0	0.0	1.9138946454586725E-4
16-17	0.0	2.55185952727823E-5	0.0	1.275929763639115E-5	2.2966735745504071E-4
18-19	0.0	2.55185952727823E-5	0.0	2.55185952727823E-5	2.2966735745504071E-4
20-21	0.0	2.55185952727823E-5	0.0	7.65557858183469E-5	2.55185952727823E-4
22-23	0.0	2.55185952727823E-5	0.0	2.041487621822584E-4	2.55185952727823E-4
24-25	0.0	2.55185952727823E-5	0.0	2.4242665509143185E-4	2.55185952727823E-4
26-27	0.0	2.55185952727823E-5	0.0	5.231312030920371E-4	2.807045480006053E-4
28-29	0.0	2.55185952727823E-5	0.0	0.0019138946454586724	3.062231432733876E-4
30-31	0.0	2.55185952727823E-5	0.0	0.0064051674134683574	3.062231432733876E-4
32-33	0.0	2.55185952727823E-5	0.0	0.01763334933349257	3.062231432733876E-4
34-35	0.0	2.55185952727823E-5	0.0	0.034360788534801366	3.1898244090977873E-4
36-37	0.0	2.55185952727823E-5	1.275929763639115E-5	0.06535312249359547	3.317417385461699E-4
38-39	0.0	2.55185952727823E-5	2.55185952727823E-5	0.11645410952734203	3.317417385461699E-4
40-41	0.0	2.55185952727823E-5	2.55185952727823E-5	0.17211016581728023	3.317417385461699E-4
42-43	0.0	5.10371905455646E-5	2.55185952727823E-5	0.23105812089740735	3.4450103618256106E-4
44-45	0.0	5.10371905455646E-5	2.55185952727823E-5	0.3054703447128405	4.2105682200090794E-4
46-47	0.0	5.10371905455646E-5	2.55185952727823E-5	0.4013947443432292	5.103719054556459E-4
48-49	0.0	5.10371905455646E-5	2.55185952727823E-5	0.5135617298647438	5.358905007284283E-4
50-51	0.0	5.10371905455646E-5	2.55185952727823E-5	0.6346729830293686	5.358905007284283E-4
52-53	0.0	5.10371905455646E-5	2.55185952727823E-5	0.7578257238158159	5.614090960012106E-4
54-55	0.0	8.931508345473806E-5	2.55185952727823E-5	0.8958302870510226	5.614090960012106E-4
56-57	0.0	1.020743810911292E-4	2.55185952727823E-5	1.0665241708306634	6.124462865467752E-4
58-59	0.0	1.020743810911292E-4	2.55185952727823E-5	1.2641784505159988	6.379648818195575E-4
60-61	0.0	1.020743810911292E-4	2.55185952727823E-5	1.4758424390060916	6.379648818195575E-4
62-63	0.0	1.020743810911292E-4	2.55185952727823E-5	1.697969051558025	6.890020723651221E-4
64-65	0.0	1.020743810911292E-4	2.55185952727823E-5	1.9275343346319747	6.890020723651221E-4
66-67	0.0	1.020743810911292E-4	2.55185952727823E-5	2.193718801922367	6.890020723651221E-4
68-69	0.0	1.020743810911292E-4	2.55185952727823E-5	2.4996229627548443	7.017613700015133E-4
70-71	0.0	1.020743810911292E-4	2.55185952727823E-5	2.839862393526851	7.272799652742956E-4
72-73	0.0	1.020743810911292E-4	2.55185952727823E-5	3.192810084744703	7.400392629106867E-4
74-75	0.0	1.020743810911292E-4	2.55185952727823E-5	3.539263293465632	7.400392629106867E-4
76-77	0.0	1.020743810911292E-4	2.55185952727823E-5	3.934023203037938	7.400392629106867E-4
78-79	0.0	1.020743810911292E-4	2.55185952727823E-5	4.369612865046696	7.400392629106867E-4
80-81	0.0	1.020743810911292E-4	2.55185952727823E-5	4.852756429346282	7.400392629106867E-4
82-83	0.0	1.020743810911292E-4	2.55185952727823E-5	5.349412089842808	7.783171558198602E-4
84-85	0.0	1.020743810911292E-4	2.55185952727823E-5	5.841920978607506	7.910764534562513E-4
86-87	0.0	1.020743810911292E-4	2.55185952727823E-5	6.363112768458812	8.676322392745983E-4
88	0.0	1.020743810911292E-4	2.55185952727823E-5	6.7827660677197175	9.186694298201629E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6770	0.0	45.979816	1
GTATCAA	11210	0.0	36.99652	1
GTGGTAT	2250	0.0	32.601685	1
GTACTAG	2860	0.0	30.580519	1
ATCAACG	13255	0.0	30.185465	3
CAACGCA	13290	0.0	30.165173	5
GGGCACC	3470	0.0	29.01043	94
AACGCAG	14750	0.0	27.94298	6
TAGGCAT	3725	0.0	27.88353	5
TATCAAC	14730	0.0	27.865387	2
CTGTGCG	3970	0.0	26.994198	9
TCAACGC	15305	0.0	26.508934	4
TAGTACT	4150	0.0	26.281773	4
GTACATG	13655	0.0	24.862421	1
ACGCAGA	16155	0.0	24.69823	7
CTAGGCA	4410	0.0	24.305862	4
CGCAGAG	16425	0.0	24.263311	8
TACATGG	13765	0.0	24.148869	2
CTAGTAC	4735	0.0	24.128723	3
ACATGGG	13455	0.0	23.65664	3
>>END_MODULE