##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146463_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5795798
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.426167199063872	31.0	30.0	34.0	27.0	34.0
2	30.54023690956793	31.0	30.0	34.0	26.0	34.0
3	30.717091244380843	31.0	31.0	34.0	27.0	34.0
4	34.235687648189256	37.0	35.0	37.0	30.0	37.0
5	34.424382630312515	37.0	35.0	37.0	30.0	37.0
6	34.577572924384185	37.0	35.0	37.0	30.0	37.0
7	34.439866951884795	37.0	35.0	37.0	30.0	37.0
8	34.37417746443199	37.0	35.0	37.0	30.0	37.0
9	35.76064434957878	39.0	35.0	39.0	30.0	39.0
10-11	35.68173459461492	39.0	35.0	39.0	29.5	39.0
12-13	35.655660273184125	39.0	35.0	39.0	30.0	39.0
14-15	36.65899907139621	40.0	36.0	41.0	28.5	41.0
16-17	36.49555471049888	40.0	36.0	41.0	28.0	41.0
18-19	36.44795850372977	40.0	36.0	41.0	27.5	41.0
20-21	36.78054566428989	40.0	36.0	41.0	30.0	41.0
22-23	36.49000827841136	39.5	35.5	41.0	28.0	41.0
24-25	36.60004454951674	39.5	35.5	41.0	29.0	41.0
26-27	36.66558410075713	39.5	35.5	41.0	29.5	41.0
28-29	36.49553892319919	39.0	35.0	41.0	28.5	41.0
30-31	36.4369634690512	39.0	35.0	41.0	29.0	41.0
32-33	36.531062331709975	39.0	35.0	41.0	29.5	41.0
34-35	36.57765746839348	39.0	35.0	41.0	29.5	41.0
36-37	36.51184254523709	39.0	35.0	41.0	29.0	41.0
38-39	36.33974467364114	39.0	35.0	41.0	29.0	41.0
40-41	36.30436093528449	39.0	35.0	41.0	28.5	41.0
42-43	36.13954549485679	39.0	35.0	41.0	28.0	41.0
44-45	35.943175900885436	39.0	34.5	41.0	28.0	41.0
46-47	35.81030455167692	38.5	34.0	40.5	27.5	41.0
48-49	35.663405108321584	38.0	34.0	40.0	27.0	41.0
50-51	35.42405083821072	38.0	34.0	40.0	27.0	41.0
52-53	35.22079021732641	38.0	33.5	40.0	26.5	41.0
54-55	35.01525018642817	37.0	33.0	40.0	26.0	41.0
56-57	34.84746242018787	37.0	33.0	40.0	26.0	41.0
58-59	34.5606276305696	36.0	33.0	40.0	26.0	41.0
60-61	34.30191148828858	36.0	33.0	39.5	26.0	41.0
62-63	34.01667043951497	35.0	32.5	39.0	26.0	41.0
64-65	33.72905102972878	35.0	32.0	39.0	25.5	41.0
66-67	33.43963428332043	35.0	32.0	38.0	25.0	40.0
68-69	33.15703851307447	35.0	32.0	37.5	25.0	40.0
70-71	33.12224399470099	35.0	32.0	37.0	25.0	40.0
72-73	32.92992026292151	35.0	32.0	37.0	25.0	40.0
74-75	32.68243423942657	35.0	31.0	36.0	25.0	39.0
76-77	32.46416050041772	35.0	31.0	36.0	24.0	39.0
78-79	32.25904957350136	35.0	31.0	35.5	24.0	39.0
80-81	32.05492134128898	35.0	31.0	35.0	24.0	37.5
82-83	31.81909298771282	35.0	31.0	35.0	23.5	37.0
84-85	31.650472721789132	34.5	31.0	35.0	23.0	37.0
86-87	31.491764550800426	34.5	31.0	35.0	23.0	36.0
88-89	31.51011750236982	35.0	31.0	35.0	23.0	36.0
90-91	31.43211547400375	35.0	31.0	35.0	23.0	36.0
92-93	31.2854667295168	35.0	31.0	35.0	23.0	36.0
94-95	31.26922996626176	35.0	31.0	35.0	23.0	35.0
96-97	31.182182591594806	34.5	31.0	35.0	23.0	35.0
98-99	31.09557915924606	34.0	31.0	35.0	22.0	35.0
100	30.586175191060835	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8386464683610626
1101	2	0.9495605283414932
1101	3	0.885506797781197
1101	4	0.9365931594346009
1101	5	0.9267380158324485
1101	6	0.6828189963046469
1101	7	0.7238629520190685
1101	8	0.7954472110032995
1101	9	0.9207650243226269
1101	10-11	0.8839313171024585
1101	12-13	0.7875511456105926
1101	14-15	1.0907402626369844
1101	16-17	1.1989474447593693
1101	18-19	1.3174297433128856
1101	20-21	0.931175434183217
1101	22-23	1.3030276000438974
1101	24-25	1.1182379769569692
1101	26-27	1.0295614310491672
1101	28-29	1.2658924048783504
1101	30-31	1.2411744542837368
1101	32-33	1.0601119710798983
1101	34-35	0.9838230906519101
1101	36-37	0.9165653943372689
1101	38-39	1.0603266055571936
1101	40-41	0.9609899420340327
1101	42-43	0.9895747782604616
1101	44-45	0.9939968587483037
1101	46-47	0.9842495501729545
1101	48-49	0.9513670507101324
1101	50-51	0.9774864515362225
1101	52-53	1.0004127305476658
1101	54-55	0.9796632445425999
1101	56-57	0.8077975781748918
1101	58-59	0.7723506950539161
1101	60-61	0.8484795023513385
1101	62-63	0.7793782602401222
1101	64-65	0.782263240715487
1101	66-67	0.7997783278386805
1101	68-69	0.8159991663019497
1101	70-71	0.8065970301717087
1101	72-73	0.7494365641031351
1101	74-75	0.7702597688091828
1101	76-77	0.7104409888896441
1101	78-79	0.6926527182561628
1101	80-81	0.6937068087331184
1101	82-83	0.7035777647716159
1101	84-85	0.7043512231313258
1101	86-87	0.7080574174542633
1101	88-89	0.6599783683967217
1101	90-91	0.7466701171552117
1101	92-93	0.8421500799391488
1101	94-95	0.781651263656542
1101	96-97	0.7992802721919432
1101	98-99	0.7880419633533577
1101	100	0.8120732604476899
1102	1	0.8007655411855517
1102	2	0.9690011053444998
1102	3	0.8734377466439796
1102	4	0.99055108017739
1102	5	0.8795363076461413
1102	6	0.6674935695361128
1102	7	0.7506381205706845
1102	8	0.789914538228679
1102	9	0.9502632323099078
1102	10-11	0.9541523837334651
1102	12-13	0.8561912721597764
1102	14-15	1.0611925179193236
1102	16-17	1.2325960561143958
1102	18-19	1.2386076816279257
1102	20-21	1.0394401148630195
1102	22-23	1.2585272504869636
1102	24-25	1.1857253480327898
1102	26-27	1.01351929618356
1102	28-29	1.1914288577303225
1102	30-31	1.264124619078558
1102	32-33	1.0974617282005568
1102	34-35	0.9588591779110374
1102	36-37	0.9516784267973009
1102	38-39	1.0098767983372596
1102	40-41	0.9853186646989514
1102	42-43	0.9552941775449995
1102	44-45	1.0151217557111352
1102	46-47	0.9383605039137493
1102	48-49	0.8956113106956494
1102	50-51	0.9917771872041925
1102	52-53	0.9930013218267675
1102	54-55	0.9567347282236085
1102	56-57	0.8985827764110397
1102	58-59	0.8583883851279168
1102	60-61	0.9582437990746016
1102	62-63	0.8539196849995108
1102	64-65	0.8212869332522956
1102	66-67	0.8998972457356089
1102	68-69	0.8425384712177646
1102	70-71	0.7887181824913654
1102	72-73	0.7686624862519125
1102	74-75	0.8235561113548044
1102	76-77	0.8057660561434687
1102	78-79	0.7920221514359582
1102	80-81	0.8719049119438438
1102	82-83	0.8677741213615846
1102	84-85	0.8174676841719517
1102	86-87	0.838227620512825
1102	88-89	0.835387743143734
1102	90-91	0.8132378418383297
1102	92-93	0.8632736254887554
1102	94-95	0.7868749218972013
1102	96-97	0.7878324443720786
1102	98-99	0.8579032372303743
1102	100	0.9069402438838878
1103	1	0.626753412179049
1103	2	0.8336280763641248
1103	3	0.7408008850129413
1103	4	0.779537208917013
1103	5	0.7548669622722173
1103	6	0.6361113121455446
1103	7	0.6309650508245639
1103	8	0.6267413670014435
1103	9	0.7875451205457082
1103	10-11	0.8634190035214004
1103	12-13	0.7371191377932718
1103	14-15	0.9264560650364828
1103	16-17	1.1085440839199165
1103	18-19	1.2211065472923082
1103	20-21	0.8574251092160665
1103	22-23	1.0935773060084983
1103	24-25	1.0330654750110995
1103	26-27	0.9501348469844118
1103	28-29	1.083936720095636
1103	30-31	1.1559742686144716
1103	32-33	0.9622843854929712
1103	34-35	0.9694264914913475
1103	36-37	0.8883239479122551
1103	38-39	1.0332736235431454
1103	40-41	0.9259022366649674
1103	42-43	0.9738661517130396
1103	44-45	1.0117342055019378
1103	46-47	0.9666402348985841
1103	48-49	0.9632818588577976
1103	50-51	0.9819748302122875
1103	52-53	0.9631198483026324
1103	54-55	0.947390794709591
1103	56-57	0.8047462071842588
1103	58-59	0.8695557675991807
1103	60-61	0.8546877626914622
1103	62-63	0.7578019441740267
1103	64-65	0.7958103058667945
1103	66-67	0.8373724042698996
1103	68-69	0.836314852612233
1103	70-71	0.7706566083791131
1103	72-73	0.7757188769269874
1103	74-75	0.7172168623843689
1103	76-77	0.8202244390492908
1103	78-79	0.8567838843806754
1103	80-81	0.7470100628927909
1103	82-83	0.7564728103234444
1103	84-85	0.7592926922991055
1103	86-87	0.7642515833080523
1103	88-89	0.7676096994162336
1103	90-91	0.7764477904127389
1103	92-93	0.8305307770020427
1103	94-95	0.7766574913460147
1103	96-97	0.7631095092222289
1103	98-99	0.8408238789987763
1103	100	0.8204878656426224
1104	1	0.5213960307849135
1104	2	0.6892927627818182
1104	3	0.6916280952660898
1104	4	0.7424933085417393
1104	5	0.6565235019060225
1104	6	0.40591652017890567
1104	7	0.3899152678522384
1104	8	0.4524282828144379
1104	9	0.6193547633579399
1104	10-11	0.5944892158920325
1104	12-13	0.45043799925165473
1104	14-15	0.6892691298612235
1104	16-17	0.8717795425741599
1104	18-19	0.8579735698967141
1104	20-21	0.6800555304771692
1104	22-23	0.9138682231126438
1104	24-25	0.8300452200624022
1104	26-27	0.7770558455324306
1104	28-29	0.9398093459002226
1104	30-31	0.9156999853998897
1104	32-33	0.7354067849104169
1104	34-35	0.7192973067033108
1104	36-37	0.6296260142760133
1104	38-39	0.7945827133009402
1104	40-41	0.727848409312557
1104	42-43	0.6701222021058868
1104	44-45	0.7345896516154937
1104	46-47	0.6665652329213643
1104	48-49	0.6031524798163943
1104	50-51	0.6948045137899683
1104	52-53	0.6791959280075588
1104	54-55	0.6591217926634982
1104	56-57	0.608309803903694
1104	58-59	0.6169580830739463
1104	60-61	0.6307862559539572
1104	62-63	0.5412942372982954
1104	64-65	0.5336306961873731
1104	66-67	0.6502844247810415
1104	68-69	0.5906040676028894
1104	70-71	0.5042404717781324
1104	72-73	0.5045762156232172
1104	74-75	0.5570395443899798
1104	76-77	0.5867973469375087
1104	78-79	0.48991895946023334
1104	80-81	0.4581309476386224
1104	82-83	0.5051394691695208
1104	84-85	0.5239583791215807
1104	86-87	0.5033503814474045
1104	88-89	0.45950067509911463
1104	90-91	0.4824139037979158
1104	92-93	0.4802961908480974
1104	94-95	0.48151124568943615
1104	96-97	0.49815295953518657
1104	98-99	0.5353837822555612
1104	100	0.6058513420511211
1105	1	0.4251800491413178
1105	2	0.5808551363379024
1105	3	0.5785708299804391
1105	4	0.6298356634332336
1105	5	0.5303306366977196
1105	6	0.2340666798075759
1105	7	0.339286894207369
1105	8	0.35876013492027425
1105	9	0.35173603937700193
1105	10-11	0.42149162830801146
1105	12-13	0.4075283681604702
1105	14-15	0.6000132846762796
1105	16-17	0.7278837446068565
1105	18-19	0.6815572832314842
1105	20-21	0.528003458927202
1105	22-23	0.7915752326913861
1105	24-25	0.6623282684520504
1105	26-27	0.5258973577621262
1105	28-29	0.7402415959424431
1105	30-31	0.6893750317424647
1105	32-33	0.5652206467097969
1105	34-35	0.5006483310871772
1105	36-37	0.43448594439351496
1105	38-39	0.5937220527744955
1105	40-41	0.4122542676872101
1105	42-43	0.45729103066057064
1105	44-45	0.45928870552798884
1105	46-47	0.43740760388542554
1105	48-49	0.4880761172580321
1105	50-51	0.5764694953881531
1105	52-53	0.5831866319401371
1105	54-55	0.4929804948877887
1105	56-57	0.450722288768695
1105	58-59	0.46340214849491446
1105	60-61	0.48253156467738734
1105	62-63	0.4516355701785386
1105	64-65	0.3976532084981983
1105	66-67	0.5258540889051773
1105	68-69	0.5326802212178592
1105	70-71	0.495321542068325
1105	72-73	0.408806751615316
1105	74-75	0.38814053828700423
1105	76-77	0.4761490088665923
1105	78-79	0.4447858862473879
1105	80-81	0.5235146980678635
1105	82-83	0.4397296709746499
1105	84-85	0.39816020389384477
1105	86-87	0.46549451437315525
1105	88-89	0.4303711603018918
1105	90-91	0.4873486351385594
1105	92-93	0.4979540630135233
1105	94-95	0.40996752396631564
1105	96-97	0.44421356026145986
1105	98-99	0.43834370517866716
1105	100	0.5079683161630584
1106	1	0.5877240418628418
1106	2	0.6246743747383441
1106	3	0.6228617272187194
1106	4	0.7774446222095364
1106	5	0.6391903595715007
1106	6	0.42317252837716524
1106	7	0.45430519193021013
1106	8	0.46275415617487425
1106	9	0.6014385236838109
1106	10-11	0.6387029619297238
1106	12-13	0.5501718864815501
1106	14-15	0.6811183473170459
1106	16-17	0.79887400985276
1106	18-19	0.7511599627004699
1106	20-21	0.6584399743584228
1106	22-23	0.9725979708105612
1106	24-25	0.7857489221336351
1106	26-27	0.6356238870232573
1106	28-29	0.8198036893968919
1106	30-31	0.7907565504331444
1106	32-33	0.7149485163146565
1106	34-35	0.6862098843424889
1106	36-37	0.5418312057706913
1106	38-39	0.6837303994605008
1106	40-41	0.6522893704773622
1106	42-43	0.6288662747148237
1106	44-45	0.6460807095652683
1106	46-47	0.5912055490978574
1106	48-49	0.6162524272917196
1106	50-51	0.674906294119296
1106	52-53	0.601382001255601
1106	54-55	0.6841928662604815
1106	56-57	0.6043401506767268
1106	58-59	0.6131591350366463
1106	60-61	0.6078671329323626
1106	62-63	0.5902843310713664
1106	64-65	0.6182733814837746
1106	66-67	0.6250939769255552
1106	68-69	0.5407965356381368
1106	70-71	0.5212630573878414
1106	72-73	0.5043957768401768
1106	74-75	0.5935383157533849
1106	76-77	0.5466753831758595
1106	78-79	0.5841956983758791
1106	80-81	0.5788264955297393
1106	82-83	0.5706571126849269
1106	84-85	0.5766571556272595
1106	86-87	0.5168191953815011
1106	88-89	0.4899670060486372
1106	90-91	0.48984449225791593
1106	92-93	0.5382200704747682
1106	94-95	0.5824598119127664
1106	96-97	0.6042284665084807
1106	98-99	0.6133070741935782
1106	100	0.5396416033154594
1107	1	0.4756418620264391
1107	2	0.6077606906234472
1107	3	0.5161483380632959
1107	4	0.6048206530741282
1107	5	0.5388262273147149
1107	6	0.34412378501609453
1107	7	0.34485150387527597
1107	8	0.357343101465176
1107	9	0.49243670524435146
1107	10-11	0.5179923164799973
1107	12-13	0.41082469291674073
1107	14-15	0.5502852201196049
1107	16-17	0.7056105553510221
1107	18-19	0.7996629347602919
1107	20-21	0.4885003141592321
1107	22-23	0.7647040278482393
1107	24-25	0.46521181347706175
1107	26-27	0.365184945954411
1107	28-29	0.5420070672605206
1107	30-31	0.5762061834125234
1107	32-33	0.49715332355167874
1107	34-35	0.372209554525071
1107	36-37	0.3644982289166663
1107	38-39	0.3971051500607672
1107	40-41	0.2813083290541698
1107	42-43	0.3859758397656279
1107	44-45	0.3887623300945222
1107	46-47	0.38645983191037203
1107	48-49	0.3388913320213973
1107	50-51	0.4207464719047067
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2214	100	-1.2048625133345503
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2215	4	-1.976055583574876
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2215	14-15	-3.8917920055096857
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2215	24-25	-5.721621901378171
2215	26-27	-3.7249655917809577
2215	28-29	-1.8484718372241247
2215	30-31	-3.9292503714061837
2215	32-33	-4.040090395647027
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2215	100	-3.3005499632651514
2216	1	-0.7442162978544289
2216	2	-0.6987050119584559
2216	3	-0.8857363526401691
2216	4	-0.8561006155697655
2216	5	-0.9194534251678945
2216	6	-0.8042089840656885
2216	7	-0.9511040007822089
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2216	9	-1.0616531769264341
2216	10-11	-1.0669651469202606
2216	12-13	-1.2400347371072655
2216	14-15	-1.6478388777399502
2216	16-17	-1.335971739526336
2216	18-19	-3.2607968562398497
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2216	32-33	-1.2095922233538303
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2216	36-37	-1.4488342090379547
2216	38-39	-1.5019466493177305
2216	40-41	-1.5580401000564024
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2216	60-61	-1.4278088770985917
2216	62-63	-1.3733166907878953
2216	64-65	-1.4546656768884674
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2216	68-69	-1.553078407230764
2216	70-71	-1.6182319237873735
2216	72-73	-1.600139092230755
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2216	80-81	-1.6384825525622517
2216	82-83	-1.714428319252999
2216	84-85	-1.6120038790186513
2216	86-87	-1.635484980650645
2216	88-89	-1.5104061387822227
2216	90-91	-1.5579503477817056
2216	92-93	-1.478795012135798
2216	94-95	-1.4272375306365994
2216	96-97	-1.4162284549662445
2216	98-99	-1.365890101486066
2216	100	-1.3221700255609115
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	23729.0
1	3937.0
2	575.0
3	629.0
4	560.0
5	329.0
6	294.0
7	365.0
8	622.0
9	1078.0
10	1953.0
11	3704.0
12	6558.0
13	9854.0
14	13147.0
15	16988.0
16	20711.0
17	24533.0
18	28780.0
19	32217.0
20	36690.0
21	41989.0
22	48394.0
23	55864.0
24	62678.0
25	71370.0
26	80718.0
27	92652.0
28	109365.0
29	129729.0
30	153728.0
31	184897.0
32	226413.0
33	285412.0
34	390001.0
35	526196.0
36	774002.0
37	1151406.0
38	869229.0
39	314283.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23440716639228	21.64443071198448	12.36991413204318	23.751247989580058
2	15.272023996352088	19.759485340848688	41.2369275809519	23.731563081847327
3	18.26566762264486	27.487580432024473	28.834930617897562	25.411821327433103
4	11.880339746836672	16.938111200905013	41.06188394537064	30.119665106887677
5	12.76348974252424	39.002495010152984	35.266824682821095	12.96719056450168
6	28.91159057352634	39.24439591411287	18.56973491943834	13.274278592922448
7	26.297666717763647	31.839133996391766	24.51344000810153	17.349759277743058
8	25.954823282800753	35.20273406902422	20.023677553821177	18.818765094353857
9	26.07238271117608	14.70760956056451	22.107400606189685	37.11260712206973
10-11	25.712015147749007	25.82818871061829	27.611702246681357	20.848093894951347
12-13	25.84859401767702	23.742324270314043	27.539620047532004	22.869461664476937
14-15	23.301714520417168	24.808849220445474	25.027986187814637	26.861450071322718
16-17	22.046667103783733	27.335939730576303	26.51933739990973	24.09805576573023
18-19	21.29845535059332	28.33692995835131	28.136830290121967	22.227784400933405
20-21	23.33682787100785	27.344580957290553	26.815569587669998	22.503021584031604
22-23	23.965477989005905	26.948168086949874	26.538477343814076	22.54787658023015
24-25	23.236719245079918	26.99772111739398	27.448198718355105	22.317360919171
26-27	23.187345946656954	27.295319483666653	26.13355123677516	23.38378333290124
28-29	22.152843354291804	27.87780960135549	26.90423357638916	23.065113467963542
30-31	23.070534791846566	27.435165643622756	26.866232058721152	22.628067505809526
32-33	23.55859292550028	27.686558013474954	25.69387945174023	23.06096960928453
34-35	23.463242366915203	27.519216977807776	26.87945906693313	22.138081588343894
36-37	23.08077047887283	27.026101686299725	26.428283750391103	23.46484408443634
38-39	23.292752536122745	27.01467165687628	27.214914355849846	22.47766145115113
40-41	22.819257363484326	26.0052020344262	27.97125254500605	23.204288057083424
42-43	22.782611290218078	26.36494238542028	27.11089742983697	23.74154889452467
44-45	22.986446801862655	27.46519924351499	26.939932793513975	22.60842116110837
46-47	22.900898772155724	27.149742387604903	26.365524386693746	23.583834453545627
48-49	23.79226028835866	26.645859013363065	26.669004644215494	22.892876054062782
50-51	23.2914530391192	26.384620048194186	26.674413100116944	23.649513812569676
52-53	22.604371719480206	27.151910842884902	26.520450080247578	23.72326735738731
54-55	22.599320537827904	27.069999140459277	26.476846652990922	23.8538336687219
56-57	23.931342187745134	26.357706684969035	26.160191328421078	23.550759798864757
58-59	22.616244715912742	26.828258848962506	27.25541769364926	23.30007874147549
60-61	22.639489407817415	27.550289798563288	26.434535843579543	23.375684950039755
62-63	23.20429819583445	26.71819261545036	25.8334449114151	24.244064277300094
64-65	22.169357428670473	26.925410916828973	26.497683962355378	24.407547692145176
66-67	23.12220937521573	26.4563978265779	26.426208517669377	23.995184280536993
68-69	22.32565200099062	26.59271445195311	26.807024361639513	24.27460918541676
70-71	22.34951562745014	26.19271796764744	26.928626341374063	24.529140063528363
72-73	23.016574116983392	26.284283519044177	27.39870469473783	23.300437669234604
74-75	23.263198859512173	26.642311234632544	26.810926431266868	23.28356347458842
76-77	22.796325486267406	26.622680707762242	26.376329621170708	24.20466418479965
78-79	22.674704267616683	26.60291098308658	26.420254532319333	24.302130216977403
80-81	22.19233636866412	26.951714919831588	27.305592565967647	23.55035614553665
82-83	23.250254040737232	25.75321333238398	28.404395214797873	22.592137412080916
84-85	23.46126184698884	26.383631848425154	26.826469324980973	23.328636979605037
86-87	21.79076552003077	27.261063775051458	27.39499983078556	23.55317087413221
88-89	23.139792018688834	25.678224770206036	27.15191568391237	24.03006752719276
90-91	22.738815735417283	25.711618879416477	27.359442961720365	24.190122423445878
92-93	23.014385484654866	26.01945011309585	27.243311853903123	23.72285254834616
94-95	21.64951924771173	26.05973282196377	28.056284496772367	24.23446343355214
96-97	22.092300215258373	25.941394492912977	27.427147396118578	24.53915789571007
98-99	21.321410295804448	26.72027823754628	27.41752927591759	24.540782190731676
100	22.250218299631637	25.868881374427737	26.355315399796197	25.52558492614443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19830.0
1	11678.5
2	3190.5
3	2176.5
4	1066.0
5	546.5
6	430.0
7	395.0
8	396.5
9	385.0
10	351.0
11	313.5
12	299.5
13	307.5
14	326.0
15	424.5
16	474.5
17	509.0
18	669.0
19	809.5
20	973.5
21	1287.5
22	1820.5
23	2607.0
24	3596.5
25	5088.0
26	7544.5
27	10225.5
28	15296.0
29	27032.0
30	43365.5
31	58941.0
32	80800.5
33	102085.0
34	118242.0
35	135969.5
36	151546.5
37	180028.0
38	192438.5
39	177369.5
40	160240.5
41	144006.0
42	141108.0
43	151929.0
44	169558.5
45	206653.5
46	243571.0
47	268565.5
48	285254.0
49	280831.5
50	267507.5
51	274407.0
52	278078.5
53	264972.0
54	250346.5
55	213751.5
56	183595.5
57	149691.5
58	114771.5
59	108588.5
60	89694.0
61	60059.0
62	43227.0
63	36685.0
64	26877.5
65	11593.0
66	5445.5
67	3575.0
68	2496.5
69	1814.0
70	1430.5
71	1155.5
72	876.5
73	672.0
74	527.0
75	377.0
76	268.5
77	188.5
78	120.5
79	73.5
80	52.5
81	39.5
82	31.0
83	19.0
84	19.0
85	23.0
86	15.5
87	11.5
88	9.5
89	9.0
90	8.0
91	9.0
92	8.0
93	6.0
94	4.0
95	3.5
96	6.5
97	5.0
98	3.0
99	2.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9932885859721128
2	0.4472378091852062
3	0.48110717454266005
4	0.46640686925251706
5	0.43272729656899706
6	0.48383328749552695
7	0.5001209496949341
8	0.5030368553217348
9	0.5166846739655178
10-11	0.5299874150203302
12-13	0.5284777005685843
14-15	0.5254496447253683
16-17	0.5305913008010286
18-19	0.5291937365656981
20-21	0.5281412499193381
22-23	0.49209789575137025
24-25	0.525768841495166
26-27	0.5060476572855024
28-29	0.5112842096981296
30-31	0.5147694933467315
32-33	0.5174610985407013
34-35	0.5288055242781063
36-37	0.5464044813156014
38-39	0.5356208066602735
40-41	0.5404346390264119
42-43	0.5390370747910814
44-45	0.5481730039590751
46-47	0.5471550250716122
48-49	0.5198766416634948
50-51	0.5347753665672959
52-53	0.5265021313717283
54-55	0.5364489928738028
56-57	0.5249234014021883
58-59	0.5302030885134368
60-61	0.5247594895474273
62-63	0.5181339998391938
64-65	0.5292023635054224
66-67	0.5264503697333828
68-69	0.5472154136496821
70-71	0.5316610413268371
72-73	0.5358451070931043
74-75	0.5330844863813404
76-77	0.5218608378000752
78-79	0.5516410337282287
80-81	0.5267350587442834
82-83	0.5259413802896512
84-85	0.5355949258411008
86-87	0.8394443698693432
88-89	1.1048176627273758
90-91	1.0952935212717902
92-93	1.095483313945724
94-95	1.0997622760489583
96-97	1.0936285219050077
98-99	1.0894530830784648
100	1.0844408310986684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5795798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.320586559844827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.703274059284	16.227797218243694
2	12.28985537883156	5.486335615823687
3	5.1730355178665794	3.4639556116107815
4	2.755221907788017	2.459926763374529
5	1.6547504987244277	1.8467500870862494
6	1.0851876378369603	1.4533214762408047
7	0.7547891756878957	1.1793135991262922
8	0.5315190186873632	0.9491053011852056
9	0.4014676812619044	0.8064894717527878
>10	2.0302304082082983	8.273969720683255
>50	0.22022993489099707	3.4841899458906
>100	0.2807528806761788	14.045759663235868
>500	0.060731515560767974	9.436926360470306
>1k	0.055013790174262285	22.982726440157066
>5k	0.003167928928574092	5.021326318972374
>10k+	7.726655923351444E-4	2.882106406146422
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	25675	0.4429933548408692	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	21864	0.3772388202625419	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	17751	0.30627361409076026	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17722	0.305773251586753	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	16866	0.291003930778816	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	16197	0.279461085427753	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	15343	0.264726272378713	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13851	0.23898348424151428	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	11582	0.19983443177281193	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	10263	0.177076564780208	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9985	0.17227998629351818	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	9641	0.1663446517632257	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9406	0.16228999009282244	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	9159	0.15802828186903684	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	9045	0.15606133961190502	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	9034	0.15587154693797126	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8565	0.1477794774766132	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8224	0.14189590458466633	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	8032	0.13858315973054963	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7937	0.1369440411829398	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7902	0.1363401554022414	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7811	0.13477005237242567	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7614	0.13137103812106635	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7564	0.1305083441486401	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	7416	0.12795476999025848	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7412	0.12788575447246436	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7329	0.12645368247823682	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	7264	0.12533218031408272	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7256	0.1251941492784945	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7098	0.12246803632562764	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	7013	0.12100145657250305	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	7007	0.1208979332958119	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6983	0.12048384018904731	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6621	0.11423793582868141	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6610	0.11404814315474762	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6591	0.11372031944522566	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6268	0.10814731638335222	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	6267	0.1081300625039037	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6245	0.10775047715603615	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	6140	0.10593881981394106	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5970	0.10300566030769188	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5928	0.10228099737085385	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	5876	0.10138379563953057	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5838	0.10072814822048663	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7253879448524603E-5
2	0.0	0.0	0.0	0.0	1.7253879448524603E-5
3	0.0	0.0	0.0	0.0	1.7253879448524603E-5
4	0.0	0.0	0.0	0.0	1.7253879448524603E-5
5	3.4507758897049206E-5	0.0	0.0	0.0	1.7253879448524603E-5
6	1.2077715613967222E-4	0.0	0.0	0.0	1.7253879448524603E-5
7	1.2077715613967222E-4	0.0	0.0	0.0	1.7253879448524603E-5
8	1.2077715613967222E-4	0.0	0.0	0.0	1.7253879448524603E-5
9	1.2077715613967222E-4	0.0	0.0	0.0	1.7253879448524603E-5
10-11	2.5880819172786906E-4	0.0	0.0	0.0	1.7253879448524603E-5
12-13	2.5880819172786906E-4	0.0	0.0	0.0	1.1215021641540991E-4
14-15	3.278237095219675E-4	0.0	0.0	8.626939724262302E-6	1.5528491503672141E-4
16-17	4.3134698621311507E-4	0.0	0.0	1.7253879448524603E-5	1.7253879448524603E-4
18-19	4.658547451101643E-4	0.0	0.0	4.313469862131151E-5	1.7253879448524603E-4
20-21	5.003625040072135E-4	0.0	0.0	9.489633696688532E-5	2.0704655338229524E-4
22-23	5.176163834557381E-4	0.0	0.0	1.5528491503672144E-4	2.0704655338229524E-4
24-25	6.038857806983612E-4	0.0	0.0	2.5880819172786906E-4	2.0704655338229524E-4
26-27	6.642743587681973E-4	0.0	0.0	4.658547451101643E-4	2.0704655338229524E-4
28-29	6.901551779409841E-4	0.0	0.0	0.00131129483808787	2.0704655338229524E-4
30-31	7.677976354593448E-4	0.0	0.0	0.005754168796082955	2.0704655338229524E-4
32-33	7.936784546321318E-4	0.0	0.0	0.01772836113335903	2.0704655338229524E-4
34-35	9.14455610771804E-4	1.7253879448524603E-5	0.0	0.03581905373513708	2.2430043283081985E-4
36-37	0.0010352327669114762	1.7253879448524603E-5	0.0	0.06823046627919055	2.4155431227934444E-4
38-39	0.0010524866463600007	1.7253879448524603E-5	0.0	0.12032855527401058	2.4155431227934444E-4
40-41	0.0010869944052570501	1.7253879448524603E-5	0.0	0.17772358525952767	2.4155431227934444E-4
42-43	0.0010869944052570501	1.7253879448524603E-5	0.0	0.24105394977533723	2.4155431227934444E-4
44-45	0.0010956213449813124	1.7253879448524603E-5	0.0	0.31645340296538976	2.9331595062491824E-4
46-47	0.0011042482847055746	2.5880819172786905E-5	0.0	0.41370489447699865	3.4507758897049206E-4
48-49	0.0011042482847055746	3.4507758897049206E-5	0.0	0.5300391766586758	3.6233146841901665E-4
50-51	0.0011042482847055746	3.4507758897049206E-5	0.0	0.6545345438195052	3.6233146841901665E-4
52-53	0.00119914462167246	3.4507758897049206E-5	0.0	0.7806086409498744	3.882122875918036E-4
54-55	0.0012077715613967221	3.4507758897049206E-5	0.0	0.9229272655810297	3.968392273160659E-4
56-57	0.0012077715613967221	3.4507758897049206E-5	0.0	1.0962252307620108	4.227200464888528E-4
58-59	0.0012077715613967221	3.4507758897049206E-5	0.0	1.3105270404524105	4.658547451101643E-4
60-61	0.0012422793202937715	3.4507758897049206E-5	0.0	1.538330010811281	4.831086245586889E-4
62-63	0.0012422793202937715	3.4507758897049206E-5	0.0	1.778202069844394	5.176163834557381E-4
64-65	0.0012422793202937715	3.4507758897049206E-5	0.0	2.032601895373165	5.176163834557381E-4
66-67	0.0012422793202937715	3.4507758897049206E-5	0.0	2.320137796382828	5.176163834557381E-4
68-69	0.001259533199742296	3.4507758897049206E-5	0.0	2.649039873370328	5.176163834557381E-4
70-71	0.001259533199742296	3.4507758897049206E-5	0.0	3.013553957539583	5.176163834557381E-4
72-73	0.001259533199742296	3.4507758897049206E-5	0.0	3.397797852858226	5.521241423527873E-4
74-75	0.001259533199742296	3.4507758897049206E-5	0.0	3.770774964896982	5.952588409740987E-4
76-77	0.001259533199742296	3.4507758897049206E-5	0.0	4.201466303691053	6.211396601468858E-4
78-79	0.0013285487175363944	3.4507758897049206E-5	0.0	4.667398691258736	6.211396601468858E-4
80-81	0.0013285487175363944	3.4507758897049206E-5	0.0	5.179252278978667	6.470204793196726E-4
82-83	0.0013371756572606568	3.4507758897049206E-5	0.0	5.709558890768795	6.901551779409841E-4
84-85	0.001345802596984919	3.4507758897049206E-5	0.0	6.232247914782399	6.901551779409841E-4
86-87	0.001345802596984919	3.4507758897049206E-5	0.0	6.790600707616104	7.160359971137711E-4
88	0.001345802596984919	3.4507758897049206E-5	0.0	7.235880201483902	7.41916816286558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6540	0.0	43.380566	94
GTACTAG	7530	0.0	35.58352	1
CAACGCA	10840	0.0	33.025513	5
TAGGCAT	9370	0.0	32.79156	5
GCATCGT	8000	0.0	32.33527	8
GTATCAA	12790	0.0	31.350464	1
CTGTGCG	9700	0.0	30.640762	9
CTAACGC	3710	0.0	30.394775	3
CTAGGCA	10370	0.0	30.356123	4
TCTAACG	3735	0.0	30.31818	2
TACCCCG	455	0.0	29.944048	5
CGCCTAC	3755	0.0	29.755728	7
TAGTACT	10310	0.0	29.62136	4
ATCAACG	12150	0.0	29.58331	3
ACTGTGC	10335	0.0	29.572319	8
TAACGCC	3835	0.0	29.525818	4
TATTCTC	3055	0.0	28.911493	5
ACGTTAA	995	0.0	28.310207	8
GTACTGT	10990	0.0	27.849396	6
TACTGTG	11880	0.0	27.583067	7
>>END_MODULE