##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5795798
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861962235398817	31.0	31.0	34.0	30.0	34.0
2	31.816198563165933	31.0	31.0	34.0	30.0	34.0
3	32.00022895898028	33.0	31.0	34.0	30.0	34.0
4	35.738769191058765	37.0	35.0	37.0	35.0	37.0
5	35.65166539620601	37.0	35.0	37.0	33.0	37.0
6	35.68813509373515	37.0	35.0	37.0	33.0	37.0
7	35.60566431059191	37.0	35.0	37.0	33.0	37.0
8	35.59155495067289	37.0	35.0	37.0	33.0	37.0
9	37.1784095995064	39.0	37.0	39.0	33.0	39.0
10-11	37.125544920647684	39.0	37.0	39.0	33.0	39.0
12-13	37.05670858439166	39.0	37.0	39.0	33.0	39.0
14-15	38.24961213279	40.0	37.5	41.0	33.0	41.0
16-17	38.25168527267513	40.0	37.0	41.0	33.0	41.0
18-19	38.300553435437195	40.0	38.0	41.0	33.0	41.0
20-21	38.31595105626525	40.0	38.0	41.0	33.0	41.0
22-23	38.2775044092289	40.0	37.5	41.0	33.0	41.0
24-25	38.20266173872865	40.0	37.0	41.0	33.0	41.0
26-27	38.055113031889654	40.0	37.0	41.0	33.0	41.0
28-29	37.994722124546094	40.0	37.0	41.0	33.0	41.0
30-31	37.87112473554116	40.0	37.0	41.0	33.0	41.0
32-33	37.75401437386189	40.0	36.5	41.0	33.0	41.0
34-35	37.66121714386871	40.0	36.0	41.0	32.0	41.0
36-37	37.58400534318139	40.0	36.0	41.0	32.0	41.0
38-39	37.46062992533557	40.0	36.0	41.0	32.0	41.0
40-41	37.33432315964083	40.0	36.0	41.0	31.0	41.0
42-43	37.20947589960865	39.0	35.5	41.0	31.0	41.0
44-45	36.999320887304904	39.0	35.0	41.0	31.0	41.0
46-47	36.75501794576002	39.0	35.0	41.0	30.5	41.0
48-49	36.48072543245986	39.0	35.0	41.0	30.0	41.0
50-51	36.26517953179183	38.5	35.0	41.0	29.5	41.0
52-53	36.060345529640614	38.0	35.0	40.0	29.0	41.0
54-55	35.848558559149225	38.0	35.0	40.0	29.0	41.0
56-57	35.56926336977237	37.5	34.0	40.0	28.5	41.0
58-59	35.26380129190147	37.0	34.0	40.0	28.0	41.0
60-61	34.99875254451587	36.5	34.0	40.0	28.0	41.0
62-63	34.71539984657851	36.0	34.0	39.0	27.5	41.0
64-65	34.441868315631424	35.0	33.0	39.0	27.0	41.0
66-67	34.2201117947865	35.0	33.0	39.0	27.0	41.0
68-69	34.03308543534471	35.0	33.0	38.0	26.0	41.0
70-71	33.85564835075343	35.0	33.0	37.0	26.0	40.0
72-73	33.67112699579937	35.0	33.0	37.0	26.0	40.0
74-75	33.50112676459739	35.0	33.0	36.5	26.5	39.0
76-77	33.36171498730632	35.0	33.0	36.0	27.0	39.0
78-79	33.25814831710836	35.0	33.0	36.0	27.0	39.0
80-81	33.125512397084925	35.0	33.0	35.5	27.0	38.0
82-83	32.93905420789338	35.0	33.0	35.0	27.0	37.0
84-85	32.80800460954643	35.0	33.0	35.0	27.0	37.0
86-87	32.68397664997987	35.0	33.0	35.0	27.0	36.5
88-89	32.58457092534971	35.0	33.0	35.0	27.0	36.0
90-91	32.49083741358825	35.0	33.0	35.0	26.0	36.0
92-93	32.40718370447003	35.0	33.0	35.0	26.0	36.0
94-95	32.37051153266556	35.0	33.0	35.0	26.0	35.0
96-97	32.31769335301196	35.0	33.0	35.0	26.0	35.0
98-99	32.2822004838678	35.0	33.0	35.0	26.0	35.0
100	31.772953267177357	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4356651028429326
1101	2	0.5707243875220378
1101	3	0.4408316507082759
1101	4	0.32918755006913614
1101	5	0.30712052126029477
1101	6	0.3501112881528812
1101	7	0.3479681822362011
1101	8	0.35777312687592655
1101	9	0.4107041060636121
1101	10-11	0.4411447615897117
1101	12-13	0.45726339200404453
1101	14-15	0.6134332695919156
1101	16-17	0.5842547796546853
1101	18-19	0.5521138512968378
1101	20-21	0.5380178660728205
1101	22-23	0.5561200809743667
1101	24-25	0.5835255681134939
1101	26-27	0.6370254810146463
1101	28-29	0.6877776475105222
1101	30-31	0.6950538508031912
1101	32-33	0.6967160826779306
1101	34-35	0.6943627738339444
1101	36-37	0.6944773589835194
1101	38-39	0.7261484180454545
1101	40-41	0.7435804546503135
1101	42-43	0.761785177772623
1101	44-45	0.6796664853142858
1101	46-47	0.6385448775582105
1101	48-49	0.6921046220968208
1101	50-51	0.6431633185552883
1101	52-53	0.7017811812185499
1101	54-55	0.6827836101233089
1101	56-57	0.6619547642708454
1101	58-59	0.6253947273608631
1101	60-61	0.6251326654167499
1101	62-63	0.6397046571037919
1101	64-65	0.6469251224519823
1101	66-67	0.6145997388568105
1101	68-69	0.6044986384940927
1101	70-71	0.48802053905570375
1101	72-73	0.4834638559007587
1101	74-75	0.4709896963254394
1101	76-77	0.5256216325992256
1101	78-79	0.5266959829675457
1101	80-81	0.4880378073344147
1101	82-83	0.44980422999489633
1101	84-85	0.4777472416959796
1101	86-87	0.45864614716796126
1101	88-89	0.4673127547436735
1101	90-91	0.4926424999623862
1101	92-93	0.510838405689988
1101	94-95	0.4887234307187782
1101	96-97	0.5382803705005159
1101	98-99	0.5002689329279946
1101	100	0.5595188721385611
1102	1	0.41705380213164034
1102	2	0.5313825503294431
1102	3	0.4237868512954961
1102	4	0.31863473283167565
1102	5	0.32830085202934356
1102	6	0.33041937415604394
1102	7	0.32952069349819624
1102	8	0.3480947321841228
1102	9	0.42293230065573795
1102	10-11	0.42259673805417464
1102	12-13	0.47541852932702966
1102	14-15	0.601551107910133
1102	16-17	0.6289516725423212
1102	18-19	0.6546300792477808
1102	20-21	0.6248953915666817
1102	22-23	0.616991139858996
1102	24-25	0.6084997102118095
1102	26-27	0.6181519156141349
1102	28-29	0.5798732704059688
1102	30-31	0.639931897722704
1102	32-33	0.6971992937288434
1102	34-35	0.6647823947502545
1102	36-37	0.6455155547059164
1102	38-39	0.6091202041585149
1102	40-41	0.6088671753924757
1102	42-43	0.7129474771976803
1102	44-45	0.6754695325506361
1102	46-47	0.7219818601900343
1102	48-49	0.6298488496225616
1102	50-51	0.5863037261208248
1102	52-53	0.5804747681226985
1102	54-55	0.606445633446242
1102	56-57	0.6693579287245157
1102	58-59	0.6669819279129143
1102	60-61	0.6351112290287375
1102	62-63	0.5779405339304304
1102	64-65	0.6043977715296762
1102	66-67	0.6482577713077973
1102	68-69	0.5768169561825047
1102	70-71	0.5974550909542344
1102	72-73	0.5831056990680352
1102	74-75	0.5562283455536559
1102	76-77	0.5316086970529739
1102	78-79	0.49114483398180653
1102	80-81	0.5030400652937246
1102	82-83	0.5335616980077802
1102	84-85	0.5055901236797098
1102	86-87	0.5125704054360867
1102	88-89	0.5090246538788534
1102	90-91	0.5687478783800728
1102	92-93	0.4943286042585058
1102	94-95	0.49493336196353255
1102	96-97	0.42117283706370756
1102	98-99	0.4435330045019441
1102	100	0.4757870551124128
1103	1	0.26846078095373826
1103	2	-0.16902453114608207
1103	3	0.20961358393898877
1103	4	0.17168793819244854
1103	5	0.2656135057100357
1103	6	0.2726329387405926
1103	7	0.32649483859753303
1103	8	0.31044411733905264
1103	9	0.36958052878324565
1103	10-11	0.39463823225986516
1103	12-13	0.43989551370586355
1103	14-15	0.5307820599660928
1103	16-17	0.5678070455927013
1103	18-19	0.5512446519358392
1103	20-21	0.46709752104924007
1103	22-23	0.46529385929802203
1103	24-25	0.47908863300022375
1103	26-27	0.5227302073001141
1103	28-29	0.5244704051623899
1103	30-31	0.5703763671650677
1103	32-33	0.606044736309336
1103	34-35	0.56236297488379
1103	36-37	0.5558234008016214
1103	38-39	0.5321718686985406
1103	40-41	0.6095636284057946
1103	42-43	0.6172632610432132
1103	44-45	0.6280822991704795
1103	46-47	0.6048286287020801
1103	48-49	0.5463582698325666
1103	50-51	0.5180218192300288
1103	52-53	0.5964961819121228
1103	54-55	0.5097000960922742
1103	56-57	0.5090271334214549
1103	58-59	0.4617617567090164
1103	60-61	0.5388061609976518
1103	62-63	0.5263715879873985
1103	64-65	0.5206226521504647
1103	66-67	0.5246643372735349
1103	68-69	0.4720574944574949
1103	70-71	0.47198407790563124
1103	72-73	0.4596334587656443
1103	74-75	0.4956174883690423
1103	76-77	0.4560760511747617
1103	78-79	0.46645820215885436
1103	80-81	0.4439174916021713
1103	82-83	0.4244092661359673
1103	84-85	0.4108991074240791
1103	86-87	0.49616509893713356
1103	88-89	0.46155925812340115
1103	90-91	0.43209916145101346
1103	92-93	0.41262339889406263
1103	94-95	0.42130748812245145
1103	96-97	0.4329985470466795
1103	98-99	0.4929336517383973
1103	100	0.508070256843105
1104	1	0.30109250648737174
1104	2	0.4005745538953853
1104	3	0.334725043108385
1104	4	0.20755043759486114
1104	5	0.2323042648382696
1104	6	0.2719636602492699
1104	7	0.30689468768537864
1104	8	0.26752564231870934
1104	9	0.3023358322467189
1104	10-11	0.325226462953637
1104	12-13	0.3471625758673156
1104	14-15	0.41859937250624313
1104	16-17	0.4583797473705431
1104	18-19	0.4324026819595872
1104	20-21	0.44852513436548236
1104	22-23	0.4327391287305744
1104	24-25	0.45297876834813877
1104	26-27	0.4967206290960462
1104	28-29	0.5184699379452269
1104	30-31	0.5359019404300511
1104	32-33	0.48772388879961426
1104	34-35	0.49979886339296087
1104	36-37	0.45262891699143637
1104	38-39	0.4591400115267277
1104	40-41	0.4792235563731211
1104	42-43	0.44324286242271427
1104	44-45	0.46482131638221347
1104	46-47	0.4647297095958649
1104	48-49	0.49728659904809547
1104	50-51	0.4476880591700123
1104	52-53	0.44220803279215204
1104	54-55	0.4651972894466354
1104	56-57	0.4110899652998441
1104	58-59	0.42975445480109187
1104	60-61	0.36819883166720047
1104	62-63	0.3964039967630626
1104	64-65	0.35422615532051793
1104	66-67	0.4237892700574548
1104	68-69	0.39748010587599225
1104	70-71	0.3661821527539715
1104	72-73	0.46865427553540684
1104	74-75	0.3566396949332571
1104	76-77	0.2907561026544272
1104	78-79	0.31763024365187675
1104	80-81	0.3250197368556158
1104	82-83	0.3028706874827165
1104	84-85	0.2800436963770423
1104	86-87	0.2968452568777238
1104	88-89	0.30700440797662054
1104	90-91	0.3327115136182144
1104	92-93	0.3225660865860078
1104	94-95	0.2642263352514931
1104	96-97	0.2866085879773834
1104	98-99	0.22201325915993664
1104	100	0.3694388650720555
1105	1	0.08513305834283358
1105	2	-0.4615731953582589
1105	3	-0.029358940398605426
1105	4	0.08710208692101418
1105	5	0.1267155649253695
1105	6	0.20311446635236052
1105	7	0.2454476131224439
1105	8	0.1806378222131002
1105	9	0.30049049729694133
1105	10-11	0.27077476969580516
1105	12-13	0.29005586651395276
1105	14-15	0.3238892246947458
1105	16-17	0.312393278177062
1105	18-19	0.31297070438566976
1105	20-21	0.3295776681931386
1105	22-23	0.32700467230739605
1105	24-25	0.3536368634885605
1105	26-27	0.31540920573490183
1105	28-29	0.3638451074087783
1105	30-31	0.42262945579976474
1105	32-33	0.3861404474228962
1105	34-35	0.43507712419818745
1105	36-37	0.3879606764886674
1105	38-39	0.4021825538294763
1105	40-41	0.4111409091853915
1105	42-43	0.3843977325895267
1105	44-45	0.45008746535910404
1105	46-47	0.3711096837368828
1105	48-49	0.3665878594436123
1105	50-51	0.37922407116269596
1105	52-53	0.3589957922301892
1105	54-55	0.39570161475337784
1105	56-57	0.42419801862607187
1105	58-59	0.38012412387455896
1105	60-61	0.3695136570553288
1105	62-63	0.27392596770895494
1105	64-65	0.2948329891725123
1105	66-67	0.29670551896490593
1105	68-69	0.33375907540494865
1105	70-71	0.32500763833687785
1105	72-73	0.34010114089984
1105	74-75	0.31873568031181065
1105	76-77	0.25762298122387506
1105	78-79	0.18094077307208067
1105	80-81	0.23423331756363552
1105	82-83	0.26265454460319404
1105	84-85	0.2625380416392886
1105	86-87	0.26625188188837257
1105	88-89	0.26110462222650455
1105	90-91	0.3047422788102452
1105	92-93	0.27207739121424623
1105	94-95	0.2661498071201578
1105	96-97	0.23388772537538927
1105	98-99	0.185669865419257
1105	100	0.23860360430611394
1106	1	0.23946582101466873
1106	2	0.2894753189925616
1106	3	0.2370181307878596
1106	4	0.18308202035061072
1106	5	0.1711681157416578
1106	6	0.2050378596153024
1106	7	0.17609980204591835
1106	8	0.21735227416482417
1106	9	0.2662587274948862
1106	10-11	0.2755861467771368
1106	12-13	0.2938155053116205
1106	14-15	0.37111100690513155
1106	16-17	0.352283303988834
1106	18-19	0.39479676026175525
1106	20-21	0.37335075612172375
1106	22-23	0.3402417090510852
1106	24-25	0.3424541827604699
1106	26-27	0.41236131992405944
1106	28-29	0.3877336375187923
1106	30-31	0.419128112409787
1106	32-33	0.4309022927106767
1106	34-35	0.3830690201956344
1106	36-37	0.39074851530624244
1106	38-39	0.42630391789323596
1106	40-41	0.4225121519074264
1106	42-43	0.40758136374317644
1106	44-45	0.4174786792028158
1106	46-47	0.39651861550323275
1106	48-49	0.33553181581338976
1106	50-51	0.34698301643403084
1106	52-53	0.39461608706519513
1106	54-55	0.3638357801152452
1106	56-57	0.358369701427641
1106	58-59	0.338291183505639
1106	60-61	0.30470311261176164
1106	62-63	0.2431813509152576
1106	64-65	0.2931765984745027
1106	66-67	0.304225481963293
1106	68-69	0.23785248010143079
1106	70-71	0.25590926319903673
1106	72-73	0.2630410224215822
1106	74-75	0.25441786450347337
1106	76-77	0.2939459664943058
1106	78-79	0.25980149612659886
1106	80-81	0.26159894810397333
1106	82-83	0.2551371455199103
1106	84-85	0.25732466297796464
1106	86-87	0.21869239905797855
1106	88-89	0.254635839035231
1106	90-91	0.2749549416061541
1106	92-93	0.23246845680782258
1106	94-95	0.2309932762953011
1106	96-97	0.21990151771552746
1106	98-99	0.2617581839174221
1106	100	0.3022260152595635
1107	1	0.013002984476226942
1107	2	-0.5380849257635667
1107	3	-0.04844452454848991
1107	4	-0.03666308213225733
1107	5	0.05088133258229277
1107	6	0.12706539245755977
1107	7	0.11739723860420526
1107	8	0.139318336544747
1107	9	0.16673523466916151
1107	10-11	0.2174581593497038
1107	12-13	0.18212414430621493
1107	14-15	0.22390729576791557
1107	16-17	0.27674145050380616
1107	18-19	0.24568532937257714
1107	20-21	0.17287049653369024
1107	22-23	0.17881990466342756
1107	24-25	0.15952403641139057
1107	26-27	0.21619442292831081
1107	28-29	0.1425557113890079
1107	30-31	0.23941066176020342
1107	32-33	0.2997723174914242
1107	34-35	0.3387928336552548
1107	36-37	0.3067964163491652
1107	38-39	0.2874362803388806
1107	40-41	0.25997926274138194
1107	42-43	0.21555405802070027
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2216	80-81	-0.6267490550216479
2216	82-83	-0.6048311425307418
2216	84-85	-0.6114907697239431
2216	86-87	-0.6123583708883231
2216	88-89	-0.5850364663537917
2216	90-91	-0.673794205615831
2216	92-93	-0.6948678198662037
2216	94-95	-0.6525917689605727
2216	96-97	-0.6552438733962802
2216	98-99	-0.6620741301032496
2216	100	-0.6494715658665982
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	14.0
13	82.0
14	348.0
15	1036.0
16	2437.0
17	5152.0
18	9004.0
19	14108.0
20	20298.0
21	28835.0
22	39587.0
23	45479.0
24	47282.0
25	50199.0
26	57361.0
27	71396.0
28	90110.0
29	113542.0
30	139247.0
31	173029.0
32	214630.0
33	271922.0
34	374843.0
35	515235.0
36	771324.0
37	1308486.0
38	1024654.0
39	405386.0
40	765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09333224733891	22.13475212062269	13.525772925468802	24.2461427065696
2	14.903108519700266	20.242320150128823	40.524550189835104	24.330021140335802
3	17.82489408923645	27.256365559112034	29.749549707791022	25.16919064386049
4	12.132670323438235	17.5444437195794	39.64834035244319	30.674545604539173
5	12.38820265499445	39.09523067587151	35.313441493894636	13.2031251752394
6	28.72098026880854	39.67106859141744	18.04735085660335	13.56060028317067
7	25.439949866920276	32.49219172452204	24.57666568779252	17.491192720765163
8	26.001320266855398	35.19558480126464	19.676479408012497	19.126615523867464
9	26.00225197634562	14.347946564045191	22.044626123960843	37.60517533564835
10-11	25.71194682768447	25.980710507854138	27.325236317759867	20.982106346701524
12-13	25.9896911521071	23.854359658497415	27.829256644210165	22.326692545185324
14-15	23.311506390368443	24.69036029985761	25.682520476994743	26.315612832779205
16-17	21.57606711871326	27.19542780553283	27.09335972557999	24.13514535017392
18-19	20.922200877256248	27.484239443817742	29.6493597602953	21.944199918630705
20-21	22.739872378174834	26.555250267198005	28.702988226551472	22.00188912807569
22-23	23.25796749567334	26.13285783560596	28.451659806325374	22.157514862395324
24-25	22.61976694149796	26.14459648179595	29.41165306313298	21.823983513573108
26-27	22.604055927151943	26.482293740999392	28.105265037662253	22.808385294186408
28-29	21.58895979466503	27.096216948899876	28.806714795788256	22.508108460646834
30-31	22.61695455914785	26.60776824865187	28.661808089239827	22.113469102960455
32-33	23.133302782464124	26.836270001128405	27.66688901165983	22.363538204747645
34-35	22.990782078324294	26.70937018776609	28.65695269398829	21.642895039921324
36-37	22.709418099112494	26.194589597498048	28.183651328082863	22.91234097530659
38-39	22.831851627679224	26.133321071576336	29.052642966507804	21.98218433423663
40-41	22.29095145408609	25.305071577189644	29.661351354289188	22.742625614435077
42-43	22.51733403637717	25.566378331848483	28.634945551917735	23.28134207985661
44-45	22.726232004635083	26.65542346368869	28.27505375446142	22.343290777214804
46-47	22.74385684249175	26.45821162159206	27.43518666454559	23.3627448713706
48-49	23.812897785235336	26.16176905109552	27.457293151677625	22.56804001199152
50-51	23.115645431021356	26.0547664061762	27.22903103498699	23.600557127815456
52-53	22.315710450916338	26.78895986368055	27.12259813057667	23.772731554826446
54-55	22.474045530929473	26.767621825176256	26.912503238350627	23.845829405543643
56-57	23.75611025311299	26.054830134488977	26.640677691793925	23.548381920604108
58-59	22.334203936911077	26.545797270862675	27.758281337954504	23.361717454271744
60-61	22.53624115489967	27.267491500516712	26.828845782721555	23.367421561862063
62-63	23.152152645761635	26.356663914097766	26.234057846736548	24.257125593404048
64-65	22.089565758638045	26.565688328482835	26.857598113115582	24.487147799763537
66-67	22.895276888532003	26.164999194243833	26.860313282139924	24.079410635084244
68-69	22.28858261443228	26.046207112347503	27.344598620755576	24.32061165246464
70-71	22.291305482091687	25.67541803088611	27.438651534643355	24.594624952378847
72-73	22.986782838187253	25.717493949927174	27.84363775273051	23.452085459155064
74-75	23.13382915342788	26.23939638892747	27.230530463086538	23.396243994558112
76-77	22.78554190840397	26.22591645934823	26.605063967554194	24.3834776646936
78-79	22.52425001755591	26.344459208087834	26.791043604394858	24.340247169961398
80-81	22.061085001800933	26.858593461864473	27.57284066179373	23.50748087454087
82-83	23.161187198224336	25.610884352802493	28.651925497817693	22.576002951155477
84-85	23.48840148650776	26.214603234858302	26.96512142042158	23.33187385821235
86-87	21.808675297031137	27.05167865558468	27.49763948764458	23.6420065597396
88-89	22.968918938972234	25.61740750620928	27.28884753014566	24.12482602467283
90-91	22.5349694557826	25.60868218958992	27.51967254314397	24.336675811483506
92-93	22.933613077722335	26.04913420647334	27.092291577100408	23.924961138703917
94-95	21.584239390653266	25.980772001383777	27.9795935445411	24.455395063421857
96-97	22.023222606647742	25.9082430358019	27.40414939721076	24.6643849603396
98-99	21.28466007704703	26.71697251798365	27.362944923987364	24.63542248098196
100	22.364504850238024	25.81482548260628	26.355849434023774	25.464820233131917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	104.0
2	90.0
3	126.5
4	89.0
5	70.0
6	136.0
7	332.0
8	622.5
9	737.5
10	560.5
11	403.0
12	422.5
13	491.0
14	640.5
15	975.5
16	1319.0
17	1844.0
18	2657.0
19	3529.5
20	4625.0
21	5812.5
22	7004.5
23	8417.5
24	9814.5
25	11334.5
26	13647.5
27	16120.0
28	20699.5
29	31683.0
30	47687.0
31	61943.5
32	83056.5
33	103879.0
34	117123.0
35	133675.0
36	148508.5
37	176897.5
38	189677.0
39	174270.5
40	157773.0
41	142166.0
42	139375.0
43	148815.5
44	164318.0
45	204034.5
46	244425.0
47	270324.5
48	288346.5
49	281304.5
50	265850.5
51	270232.5
52	272283.0
53	261453.0
54	251936.5
55	215462.5
56	183661.5
57	149222.5
58	113420.5
59	111713.5
60	92567.5
61	59333.0
62	41279.0
63	34811.0
64	25129.0
65	9920.5
66	4369.5
67	2972.5
68	2053.5
69	1460.0
70	1167.0
71	984.5
72	738.5
73	580.5
74	448.0
75	288.5
76	201.5
77	129.0
78	77.0
79	36.0
80	16.5
81	7.5
82	3.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013837611317716733
2	0.004175438826542954
3	0.0020187038954773786
4	0.027882269188815756
5	0.0026743513145213132
6	0.0
7	4.140931067645905E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008868494036541646
16-17	0.0027002321336941004
18-19	0.0
20-21	4.313469862131151E-5
22-23	8.02305394356394E-4
24-25	0.0
26-27	1.4665797531245915E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.968392273160659E-4
36-37	0.0
38-39	0.0
40-41	4.658547451101643E-4
42-43	8.195592738049187E-4
44-45	0.0
46-47	0.0
48-49	6.125127204226234E-4
50-51	8.626939724262302E-6
52-53	0.0
54-55	4.0546616704032824E-4
56-57	1.2077715613967222E-4
58-59	1.8116573420950833E-4
60-61	7.764245751836072E-5
62-63	0.0
64-65	8.626939724262302E-6
66-67	0.0
68-69	0.0015269683311944274
70-71	9.317094902203286E-4
72-73	0.0
74-75	2.2430043283081985E-4
76-77	0.002838263169282297
78-79	5.003625040072135E-4
80-81	0.0032005946377013142
82-83	0.0017598957037495097
84-85	0.005788676554980005
86-87	0.007695230234041972
88-89	0.007522691439556727
90-91	0.0059957231083623
92-93	0.008756343820126236
94-95	0.0014320719942275421
96-97	0.004856967064759676
98-99	0.0020187038954773786
100	0.08181789634490368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5795798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.817037649123483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.890989645712416	10.10566188665992
2	15.73424655829078	4.977383403861537
3	6.907412479057175	3.277644097178181
4	3.7572409629350587	2.3771368707029117
5	2.2463134866883827	1.7765012495342
6	1.4529321078090505	1.3788649110501654
7	1.011189972872861	1.1195820909942338
8	0.7034774449295739	0.890154338540821
9	0.5490253504088798	0.7815559173966419
>10	2.8154391407770283	8.16321693348533
>50	0.32058178212259913	3.604345589930793
>100	0.42010245204286234	14.785015123611498
>500	0.09443384803770982	10.309229117563184
>1k	0.08985391545388026	26.966977530696003
>5k	0.005670392722817687	6.5234297330263615
>10k+	0.0010904601390034011	2.963301205768276
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	28918	0.49894768589243443	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	22126	0.38175933667805534	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	18672	0.3221644370628514	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	18334	0.3163326258092501	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	18240	0.31471076114108876	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17621	0.30403060976245205	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	14638	0.25256228736750314	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	12433	0.2145174831835064	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	10708	0.18475454113480147	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	10117	0.17455749838072343	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	9767	0.1685186405737398	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9725	0.16779397763690176	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9652	0.16653444443715948	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9627	0.16610309745094637	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	9532	0.16446397890333653	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	9489	0.16372206208704995	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9401	0.1622037206955798	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	9133	0.15757968100337522	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	9105	0.15709657237881652	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9104	0.157079318499368	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	8843	0.15257605596330306	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	8700	0.15010875120216405	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	8550	0.14752066928488536	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8508	0.14679600634804732	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8438	0.14558823478665062	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	8438	0.14558823478665062	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	8427	0.14539844211271682	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	8238	0.14213745889694568	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7834	0.13516689159974174	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	7516	0.12968015793511092	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7380	0.12733363033011158	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	7370	0.12716109153562632	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	7284	0.1256772579030532	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	7208	0.12436596306496533	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	7206	0.12433145530606829	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	7203	0.12427969366772271	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	7113	0.12272684451735551	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	7030	0.12129477252312795	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6929	0.11955213069882699	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6927	0.11951762293992993	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6708	0.11573902334070303	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6687	0.11537669187228403	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6612	0.11408265091364467	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	6573	0.11340974961515221	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	6407	0.11054560562669714	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	6211	0.10716384525478631	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	6197	0.10692229094250696	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	6177	0.10657721335353648	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	6076	0.10483457152923549	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	6025	0.10395462367736073	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5930	0.10231550512975089	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5903	0.10184965038464074	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7253879448524603E-5	0.0	0.0	0.0	0.0
2	3.4507758897049206E-5	0.0	0.0	0.0	0.0
3	3.4507758897049206E-5	0.0	0.0	0.0	0.0
4	3.4507758897049206E-5	0.0	0.0	0.0	0.0
5	3.4507758897049206E-5	0.0	0.0	0.0	0.0
6	5.176163834557381E-5	0.0	0.0	0.0	1.7253879448524603E-5
7	5.176163834557381E-5	0.0	0.0	0.0	1.7253879448524603E-5
8	6.901551779409841E-5	0.0	0.0	0.0	1.7253879448524603E-5
9	6.901551779409841E-5	0.0	0.0	0.0	1.7253879448524603E-5
10-11	2.2430043283081985E-4	0.0	0.0	0.0	3.4507758897049206E-5
12-13	2.5880819172786906E-4	0.0	0.0	8.626939724262302E-6	7.764245751836071E-5
14-15	2.84689010900656E-4	0.0	0.0	1.7253879448524603E-5	8.626939724262302E-5
16-17	3.450775889704921E-4	0.0	0.0	1.7253879448524603E-5	9.489633696688532E-5
18-19	3.795853478675413E-4	0.0	0.0	4.313469862131151E-5	1.3803103558819683E-4
20-21	4.054661670403282E-4	0.0	0.0	9.489633696688532E-5	1.6391185476098374E-4
22-23	4.8310862455868894E-4	0.0	0.0	1.5528491503672144E-4	1.7253879448524603E-4
24-25	7.419168162865579E-4	0.0	0.0	2.4155431227934444E-4	1.7253879448524603E-4
26-27	8.626939724262301E-4	0.0	0.0	4.486008656616397E-4	1.7253879448524603E-4
28-29	8.626939724262301E-4	0.0	0.0	0.0013371756572606568	1.7253879448524603E-4
30-31	9.230825504960662E-4	0.0	0.0	0.005900826771395415	1.7253879448524603E-4
32-33	0.0010179788874629515	0.0	0.0	0.01803030402370821	1.7253879448524603E-4
34-35	0.0011042482847055746	0.0	0.0	0.03635392399804134	2.2430043283081985E-4
36-37	0.0011905176819481977	0.0	0.0	0.06945549172003579	2.2430043283081985E-4
38-39	0.0011905176819481977	0.0	0.0	0.12219197425445125	2.2430043283081985E-4
40-41	0.001345802596984919	0.0	0.0	0.1805704753685342	2.2430043283081985E-4
42-43	0.001345802596984919	0.0	0.0	0.24494469959097953	2.2430043283081985E-4
44-45	0.0013889372956062307	0.0	0.0	0.32197464438891765	2.3292737255508215E-4
46-47	0.0014320719942275421	0.0	0.0	0.42085662750841213	2.5018125200360677E-4
48-49	0.0014320719942275421	0.0	0.0	0.5393562715608791	2.5880819172786906E-4
50-51	0.0014320719942275421	0.0	0.0	0.6656546691240792	2.5880819172786906E-4
52-53	0.0015269683311944274	0.0	0.0	0.7941615632566905	2.7606207117639365E-4
54-55	0.0015355952709186897	0.0	0.0	0.9382745913504922	2.7606207117639365E-4
56-57	0.0015355952709186897	0.0	0.0	1.1134877371502596	2.9331595062491824E-4
58-59	0.0015528491503672144	0.0	0.0	1.3309901414783607	2.9331595062491824E-4
60-61	0.0015528491503672144	0.0	0.0	1.561855675439344	2.9331595062491824E-4
62-63	0.0015528491503672144	0.0	0.0	1.8043071894500118	2.9331595062491824E-4
64-65	0.0015528491503672144	0.0	0.0	2.061536651208341	2.9331595062491824E-4
66-67	0.0015528491503672144	0.0	0.0	2.353687964970484	2.9331595062491824E-4
68-69	0.0016218646681613127	0.0	0.0	2.686920765699564	2.9331595062491824E-4
70-71	0.0016218646681613127	0.0	0.0	3.056774925558137	3.019428903491806E-4
72-73	0.0016218646681613127	0.0	0.0	3.4468937668290027	3.105698300734429E-4
74-75	0.0016218646681613127	0.0	0.0	3.8260909024089518	3.105698300734429E-4
76-77	0.0016391185476098374	0.0	0.0	4.266254621020263	3.105698300734429E-4
78-79	0.0017081340654039358	0.0	0.0	4.743341641651417	3.105698300734429E-4
80-81	0.0017081340654039358	0.0	0.0	5.267065898431933	3.191967697977052E-4
82-83	0.0017512687640252472	0.0	0.0	5.809536495233271	3.278237095219675E-4
84-85	0.0017944034626465588	0.0	0.0	6.344182457704703	3.278237095219675E-4
86-87	0.0017944034626465588	0.0	0.0	6.914207499985334	3.623314684190167E-4
88	0.0017944034626465588	0.0	0.0	7.369891083160593	3.968392273160659E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6835	0.0	43.41986	94
GGTATCA	12750	0.0	39.679367	1
TACCCCG	400	0.0	36.423416	5
GTATCAA	20540	0.0	34.45075	1
GTACTAG	7405	0.0	32.255318	1
TATTCTC	3395	0.0	31.977991	5
CTGTGCG	10015	0.0	30.741798	9
GTGGTAT	3415	0.0	30.014317	1
ACTGTGC	10620	0.0	29.826302	8
CAGTACG	1130	0.0	29.53377	9
CAACGCA	22545	0.0	29.518347	5
TAGTACT	10615	0.0	29.229599	4
TAGGCAT	9350	0.0	29.20408	5
ATCAACG	23410	0.0	28.495737	3
GTACTGT	11195	0.0	28.252619	6
TACTGTG	12085	0.0	28.155268	7
TATCCGC	220	3.6379788E-12	27.783266	1
TATCAAC	26045	0.0	27.130156	2
TATTCGC	495	0.0	26.588377	9
CTAGGCA	10680	0.0	26.278584	4
>>END_MODULE