FastQCFastQC Report
Mon 16 Jan 2017
ERR1146462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4068037
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122590.30134927484681184No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC86990.2138377797448745No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT75170.18478199682057955No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66130.16255997671604264No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57530.14141955935995668No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA53070.13045604059156787No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC50410.1239172603395692No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC44450.10926645947418866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68150.034.4054831
GTACTAG27350.033.2917861
CAACGCA63850.033.033565
ATCAACG66350.031.5111473
GGTATCA43500.030.1503721
TAGGCAT37050.028.147175
AACGCAG78700.027.6179686
TATCAAC83350.027.3949452
TAGTACT39700.026.1522664
GTACATG143750.025.9600641
GCATCGT30600.025.928838
GGGCACC36400.025.68685794
CTGTGCG39700.025.5457389
ACGCAGA82700.025.2054887
TCTAACG14200.025.1457732
CTAGGCA42350.025.0704734
TACATGG143300.025.04882
TCAACGC85600.025.0264134
CGCAGAG83850.024.8038048
ACATGGG140800.024.7931253
Produced by FastQC (version 0.11.5)