##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146462_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4068037
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44941724964645	31.0	30.0	34.0	27.0	34.0
2	30.549668550212303	31.0	30.0	34.0	26.0	34.0
3	30.76746327528486	31.0	31.0	34.0	27.0	34.0
4	34.21586037688448	37.0	35.0	37.0	30.0	37.0
5	34.45648503196996	37.0	35.0	37.0	30.0	37.0
6	34.579789957662626	37.0	35.0	37.0	30.0	37.0
7	34.48237909340549	37.0	35.0	37.0	30.0	37.0
8	34.407506863875625	37.0	35.0	37.0	30.0	37.0
9	35.81147369111933	39.0	35.0	39.0	30.0	39.0
10-11	35.74295010099466	39.0	35.0	39.0	30.0	39.0
12-13	35.72833654167846	39.0	35.0	39.0	30.0	39.0
14-15	36.75983269080395	40.0	36.0	41.0	29.5	41.0
16-17	36.586805749308574	40.0	36.0	41.0	28.5	41.0
18-19	36.53765931824121	40.0	36.0	41.0	27.5	41.0
20-21	36.86478527604346	40.0	36.0	41.0	30.0	41.0
22-23	36.599609344752764	40.0	36.0	41.0	28.5	41.0
24-25	36.71514467542945	40.0	36.0	41.0	29.5	41.0
26-27	36.79244325948854	40.0	36.0	41.0	30.0	41.0
28-29	36.62073489007106	40.0	36.0	41.0	29.5	41.0
30-31	36.56113169570483	40.0	35.5	41.0	29.0	41.0
32-33	36.65282493743297	40.0	35.5	41.0	29.5	41.0
34-35	36.690491507328964	40.0	35.0	41.0	30.0	41.0
36-37	36.621153642407876	40.0	35.0	41.0	30.0	41.0
38-39	36.469873184535935	39.0	35.0	41.0	29.0	41.0
40-41	36.44103593453059	39.0	35.0	41.0	29.0	41.0
42-43	36.28835135963611	39.0	35.0	41.0	28.0	41.0
44-45	36.136600773296806	39.0	35.0	41.0	28.0	41.0
46-47	36.023635846969924	39.0	35.0	41.0	28.0	41.0
48-49	35.91199821928856	39.0	34.5	41.0	28.0	41.0
50-51	35.72086610814995	38.5	34.0	40.0	27.0	41.0
52-53	35.55568840696385	38.0	34.0	40.0	27.0	41.0
54-55	35.37584601614981	38.0	34.0	40.0	27.0	41.0
56-57	35.25352842169332	38.0	34.0	40.0	27.0	41.0
58-59	35.03045744175876	37.0	33.0	40.0	26.5	41.0
60-61	34.78028493841133	37.0	33.0	40.0	26.0	41.0
62-63	34.520475231665785	36.0	33.0	40.0	26.0	41.0
64-65	34.24123514609134	36.0	33.0	39.0	26.0	41.0
66-67	33.94298871912915	35.0	32.0	39.0	26.0	41.0
68-69	33.655421767304475	35.0	32.0	39.0	25.0	40.5
70-71	33.54852463239641	35.0	32.0	38.0	26.0	40.0
72-73	33.30968892367498	35.0	32.0	37.5	25.5	40.0
74-75	33.02832926544178	35.0	32.0	37.0	25.0	39.5
76-77	32.769725422851366	35.0	32.0	36.5	25.0	39.0
78-79	32.53808814914909	35.0	31.5	36.0	25.0	39.0
80-81	32.28019718109742	35.0	31.0	36.0	24.5	37.5
82-83	32.04954551789966	35.0	31.0	35.0	24.0	37.0
84-85	31.847536662031345	35.0	31.0	35.0	24.0	37.0
86-87	31.669763819748937	34.5	31.0	35.0	24.0	36.0
88-89	31.647627467498452	35.0	31.0	35.0	24.0	36.0
90-91	31.56563804606497	35.0	31.0	35.0	24.0	36.0
92-93	31.43061198804239	35.0	31.0	35.0	23.0	36.0
94-95	31.38416575365465	35.0	31.0	35.0	23.0	35.0
96-97	31.297040194078864	35.0	31.0	35.0	23.0	35.0
98-99	31.208095698244634	34.5	31.0	35.0	23.0	35.0
100	30.719695764812364	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.921851354672885
1101	2	1.0576724713647785
1101	3	1.0332658459960733
1101	4	1.0977881063438915
1101	5	0.9538594174950816
1101	6	0.7556960910179455
1101	7	0.765488806486573
1101	8	0.8453023030086086
1101	9	1.023051377621016
1101	10-11	1.0588453205634636
1101	12-13	0.9295064293888728
1101	14-15	1.192562365128616
1101	16-17	1.3018314058978433
1101	18-19	1.4155388788694552
1101	20-21	1.1603123338016275
1101	22-23	1.4625675060337358
1101	24-25	1.2619862560663506
1101	26-27	1.1943906393859223
1101	28-29	1.4265625867853302
1101	30-31	1.4425485734447747
1101	32-33	1.2465675172681898
1101	34-35	1.1714734533018287
1101	36-37	1.210520662237002
1101	38-39	1.2551522416445735
1101	40-41	1.1463687114723626
1101	42-43	1.1790346171832269
1101	44-45	1.175093596034067
1101	46-47	1.182909685183347
1101	48-49	1.1430806259279365
1101	50-51	1.1316262138637754
1101	52-53	1.1914279127645955
1101	54-55	1.1134806901393404
1101	56-57	0.9484654571980116
1101	58-59	0.9510212899135482
1101	60-61	0.9476484558332174
1101	62-63	0.959088954265944
1101	64-65	0.9178884525372055
1101	66-67	0.9317303603072347
1101	68-69	0.9176799491457004
1101	70-71	0.8138444517630887
1101	72-73	0.8090792601427452
1101	74-75	0.7814616457367052
1101	76-77	0.8351863411630092
1101	78-79	0.8428915280667013
1101	80-81	0.7900538609497474
1101	82-83	0.8247732600190574
1101	84-85	0.8369939803560094
1101	86-87	0.848307828126746
1101	88-89	0.7837921884461849
1101	90-91	0.8194828972819472
1101	92-93	0.8414076248428266
1101	94-95	0.822977863429923
1101	96-97	0.8124785153235301
1101	98-99	0.8366701738217017
1101	100	0.8667202251978985
1102	1	0.7449233034850806
1102	2	0.9220142365131601
1102	3	0.9345873423247184
1102	4	1.032084468963724
1102	5	0.838151911242349
1102	6	0.6611827340990359
1102	7	0.6953752361687933
1102	8	0.7424979230957973
1102	9	0.8546895338967033
1102	10-11	0.8742681010669102
1102	12-13	0.798350704068362
1102	14-15	1.0999958074303464
1102	16-17	1.2909489655177495
1102	18-19	1.2442339285161097
1102	20-21	0.9767875639995438
1102	22-23	1.2573657050590086
1102	24-25	1.1211192969943653
1102	26-27	0.9260981444750911
1102	28-29	1.1625601043072464
1102	30-31	1.1911253083141418
1102	32-33	1.052454908641593
1102	34-35	0.9692387576017438
1102	36-37	0.9302873866749621
1102	38-39	1.0232973266928624
1102	40-41	0.9127730220363617
1102	42-43	0.9709739957317609
1102	44-45	1.0480541474967637
1102	46-47	1.0127224172066178
1102	48-49	0.8839918297756313
1102	50-51	1.0203313313058757
1102	52-53	0.9890154816407062
1102	54-55	0.9621112692426621
1102	56-57	0.8957573773049248
1102	58-59	0.8940237793730503
1102	60-61	0.9197378128265967
1102	62-63	0.9005622936614728
1102	64-65	0.8096681600969831
1102	66-67	0.8632422255285093
1102	68-69	0.8274121708035906
1102	70-71	0.7509677093886893
1102	72-73	0.7594752232505684
1102	74-75	0.7892562603824516
1102	76-77	0.731274751009849
1102	78-79	0.7911218679398928
1102	80-81	0.8013078439976837
1102	82-83	0.8153132120915814
1102	84-85	0.6862952125191946
1102	86-87	0.7193916127800613
1102	88-89	0.7665022551362384
1102	90-91	0.8361123587756119
1102	92-93	0.9333446907945948
1102	94-95	0.870877705591564
1102	96-97	0.7796407322049639
1102	98-99	0.8113689108238837
1102	100	0.8380822777831511
1103	1	0.662231043762965
1103	2	0.8578534845708461
1103	3	0.8242757183803029
1103	4	0.8231126044392099
1103	5	0.7869245939464093
1103	6	0.5897147791449626
1103	7	0.6434478898988587
1103	8	0.6898100094728363
1103	9	0.818447384218203
1103	10-11	0.9476959500644213
1103	12-13	0.7663488552972595
1103	14-15	1.0082946741825936
1103	16-17	1.2580474598294416
1103	18-19	1.341829950444243
1103	20-21	1.0127047396299176
1103	22-23	1.154314249355636
1103	24-25	1.0900429131023657
1103	26-27	0.9786115905191863
1103	28-29	1.1748387698533307
1103	30-31	1.196900103625346
1103	32-33	1.0430545743853088
1103	34-35	1.0027863497860636
1103	36-37	1.0239285063375547
1103	38-39	1.0572714218311816
1103	40-41	0.970354374680575
1103	42-43	1.0419244242974273
1103	44-45	1.0323965577493297
1103	46-47	1.015229315319857
1103	48-49	1.0435888178233768
1103	50-51	1.0735751714538608
1103	52-53	1.0101734532308342
1103	54-55	1.0303061941885048
1103	56-57	0.9091039552075486
1103	58-59	0.9056018403028645
1103	60-61	1.0148903292467963
1103	62-63	0.9075981053266773
1103	64-65	0.804884500854179
1103	66-67	0.9007632604565643
1103	68-69	0.903431129581854
1103	70-71	0.7593864776293628
1103	72-73	0.7412338631613835
1103	74-75	0.7338904589061244
1103	76-77	0.7560482837456561
1103	78-79	0.7368711575013833
1103	80-81	0.7386675551787718
1103	82-83	0.71729454880969
1103	84-85	0.6424159448512903
1103	86-87	0.7536024887732857
1103	88-89	0.6895442559152727
1103	90-91	0.7260987496328788
1103	92-93	0.7474325280490142
1103	94-95	0.7441842379800754
1103	96-97	0.7055849534281293
1103	98-99	0.7974568843136396
1103	100	0.7949912322824666
1104	1	0.626837124181403
1104	2	0.7686854558032756
1104	3	0.7345944499919383
1104	4	0.8697716433408118
1104	5	0.7615976897293422
1104	6	0.5080851616228941
1104	7	0.5268150033346259
1104	8	0.564561562267869
1104	9	0.6838903394323239
1104	10-11	0.7267667248274208
1104	12-13	0.6478207415345949
1104	14-15	0.8726040890240441
1104	16-17	0.9546901535201897
1104	18-19	0.9623399210228598
1104	20-21	0.8627496889597666
1104	22-23	1.076828960045404
1104	24-25	0.9218588660281597
1104	26-27	0.8089515709049309
1104	28-29	1.0164250725666335
1104	30-31	1.041393402088893
1104	32-33	0.9175266240747533
1104	34-35	0.8003746139246672
1104	36-37	0.7811383788449078
1104	38-39	0.9046259494545836
1104	40-41	0.8271294645792366
1104	42-43	0.7929419136730402
1104	44-45	0.8608004479095186
1104	46-47	0.7896776644535635
1104	48-49	0.7277396841195554
1104	50-51	0.9240655277794545
1104	52-53	0.90716103087059
1104	54-55	0.8338146936981445
1104	56-57	0.793808924151655
1104	58-59	0.7648949099334672
1104	60-61	0.7827556447093684
1104	62-63	0.6886973207565532
1104	64-65	0.6661210160552287
1104	66-67	0.8301911780767028
1104	68-69	0.7548605182709665
1104	70-71	0.6804096878287709
1104	72-73	0.7049793682284005
1104	74-75	0.6948989584157701
1104	76-77	0.6827727034906772
1104	78-79	0.6360972117674848
1104	80-81	0.6284109353683718
1104	82-83	0.5770747057237173
1104	84-85	0.48070705305658734
1104	86-87	0.4782992851119623
1104	88-89	0.4857913955033766
1104	90-91	0.5255454642455
1104	92-93	0.5297944110173027
1104	94-95	0.5094776345148873
1104	96-97	0.533673381018847
1104	98-99	0.5995000356702924
1104	100	0.7041641646132923
1105	1	0.48057702950025316
1105	2	0.672845624744145
1105	3	0.683509097722375
1105	4	0.7473199121610108
1105	5	0.6613609868610624
1105	6	0.30541622075219976
1105	7	0.389667504959057
1105	8	0.4443763770826834
1105	9	0.5110457279035003
1105	10-11	0.5848379445433736
1105	12-13	0.49835320695741103
1105	14-15	0.6927533046557173
1105	16-17	0.8466511903715812
1105	18-19	0.8347426871975614
1105	20-21	0.6470315742284996
1105	22-23	0.9211270896286265
1105	24-25	0.7338135211845724
1105	26-27	0.5987691704593701
1105	28-29	0.824531565651192
1105	30-31	0.79316716520038
1105	32-33	0.7038493753975033
1105	34-35	0.6158106577379101
1105	36-37	0.49153787295054485
1105	38-39	0.6965691175600384
1105	40-41	0.5522930680323128
1105	42-43	0.5779478312447495
1105	44-45	0.7310153369840506
1105	46-47	0.6399561384162453
1105	48-49	0.7091378554738696
1105	50-51	0.703939982434612
1105	52-53	0.7376195846624753
1105	54-55	0.6384545340397381
1105	56-57	0.6316065431992683
1105	58-59	0.5689630664358276
1105	60-61	0.5618642959671405
1105	62-63	0.5988880754209944
1105	64-65	0.5105752070883653
1105	66-67	0.681897759344686
1105	68-69	0.5743013657600571
1105	70-71	0.5748679399405319
1105	72-73	0.468692934551612
1105	74-75	0.5257138428490791
1105	76-77	0.5010539389868214
1105	78-79	0.5304294544111272
1105	80-81	0.6204773282023055
1105	82-83	0.5376145971188748
1105	84-85	0.47362952126414726
1105	86-87	0.5636549195684672
1105	88-89	0.5378333058347593
1105	90-91	0.5224228428772655
1105	92-93	0.5793352770713263
1105	94-95	0.4698221300128935
1105	96-97	0.4628538553528294
1105	98-99	0.5188696402372628
1105	100	0.6275750065329078
1106	1	0.4901630861451345
1106	2	0.5051322288444773
1106	3	0.5625416936667378
1106	4	0.693301281916888
1106	5	0.5063668524551588
1106	6	0.23349914908023095
1106	7	0.2792909896805824
1106	8	0.3840487417550449
1106	9	0.5619129978884203
1106	10-11	0.507723188384503
1106	12-13	0.34690601127001486
1106	14-15	0.5197971848858671
1106	16-17	0.6609656839752915
1106	18-19	0.6850960194136348
1106	20-21	0.43609538351289956
1106	22-23	0.77946496181454
1106	24-25	0.5280156607298494
1106	26-27	0.42324964641043294
1106	28-29	0.6206222170114515
1106	30-31	0.6022885131697606
1106	32-33	0.5583281754645384
1106	34-35	0.508369034364911
1106	36-37	0.40811271513813097
1106	38-39	0.5330003923491518
1106	40-41	0.4239852919304212
1106	42-43	0.4925353542881581
1106	44-45	0.4795475418888131
1106	46-47	0.4484444910222436
1106	48-49	0.49411285687043716
1106	50-51	0.5117921973946622
1106	52-53	0.4237544374639981
1106	54-55	0.4640438865898773
1106	56-57	0.42514915255070207
1106	58-59	0.41420041351141634
1106	60-61	0.34439390325165675
1106	62-63	0.3277761263678727
1106	64-65	0.39222008726266466
1106	66-67	0.43897138651635004
1106	68-69	0.47211678109997735
1106	70-71	0.4445138396109414
1106	72-73	0.37757477076188195
1106	74-75	0.3238525641495542
1106	76-77	0.3621039501593799
1106	78-79	0.3728778511487434
1106	80-81	0.43881534004522393
1106	82-83	0.3952283477473273
1106	84-85	0.41315559522649536
1106	86-87	0.40943413581337396
1106	88-89	0.39272479234389124
1106	90-91	0.3348439366709073
1106	92-93	0.43666203255984826
1106	94-95	0.44639152557738626
1106	96-97	0.46504751771805886
1106	98-99	0.4506936305171365
1106	100	0.41662450097456016
1107	1	0.49512372913000036
1107	2	0.6246246928020724
1107	3	0.5493059859612899
1107	4	0.6405989937601078
1107	5	0.5574582279442026
1107	6	0.35735333361979826
1107	7	0.33578892670463034
1107	8	0.38159070673668793
1107	9	0.49797511263138006
1107	10-11	0.5026417068610058
1107	12-13	0.4409786127089248
1107	14-15	0.5689107362437795
1107	16-17	0.6450584826790333
1107	18-19	0.7932859086808222
1107	20-21	0.5701577421544854
1107	22-23	0.7909761974589031
1107	24-25	0.531523854258694
1107	26-27	0.5121589704756175
1107	28-29	0.6684906800199215
1107	30-31	0.6451294501375671
1107	32-33	0.5499915925127681
1107	34-35	0.4704843445344835
1107	36-37	0.46671562500009145
1107	38-39	0.534638838147643
1107	40-41	0.4049053049950686
1107	42-43	0.5116477192399245
1107	44-45	0.5026884647237253
1107	46-47	0.4391390254712775
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2216	94-95	-1.3856366017974153
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2216	98-99	-1.388861642242638
2216	100	-1.2944907625418693
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16341.0
1	2822.0
2	412.0
3	426.0
4	385.0
5	240.0
6	191.0
7	264.0
8	468.0
9	815.0
10	1420.0
11	2738.0
12	4611.0
13	6733.0
14	8944.0
15	11343.0
16	14103.0
17	16456.0
18	19196.0
19	21604.0
20	24888.0
21	28216.0
22	32148.0
23	37437.0
24	41904.0
25	46741.0
26	53318.0
27	61004.0
28	72172.0
29	86131.0
30	102521.0
31	122517.0
32	150817.0
33	190864.0
34	257880.0
35	349007.0
36	512800.0
37	788173.0
38	743813.0
39	235809.0
40	365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3695002373391	19.71342048345677	12.582398376163606	24.334680903040525
2	15.547080589161052	19.589822270967268	40.56980843426505	24.29328870560663
3	18.887146793441158	25.577904763033853	28.745735735646733	26.789212707878256
4	12.307022116811952	16.311281552184877	38.944915533628375	32.436780797374794
5	13.448730023341845	37.932339504747986	35.0576016530619	13.561328818848265
6	31.21390124427706	37.415501988827785	17.868600035172513	13.501996731722643
7	27.713207785816913	31.45567068579458	22.872344104806945	17.958777423581562
8	25.972470879049887	35.345376407626844	20.0258951707557	18.656257542567563
9	26.01565833625915	15.465862311698586	20.771991013270323	37.74648833877194
10-11	25.358618336977617	25.833963160281968	27.915647792615623	20.89177071012479
12-13	25.6713499451049	23.55847533204557	27.795678026810755	22.97449669603878
14-15	22.990485253198813	25.02962431878893	25.051418625261107	26.928471802751147
16-17	22.517921877664186	27.69853684332782	26.2349600788785	23.548581200129487
18-19	22.130228779085428	27.91909431336374	27.559525015654394	22.39115189189643
20-21	23.47935641725115	27.164131195042422	26.833253661614588	22.523258726091843
22-23	23.64969129131503	26.837276743534037	26.593002273785515	22.92002969136542
24-25	23.184292625900547	26.92342524162544	27.085363429348458	22.806918703125554
26-27	23.117240892023037	27.17003031071884	26.406407224964628	23.3063215722935
28-29	22.55484531833929	27.491245156855854	26.865435083709	23.088474441095855
30-31	23.11110485189627	27.236343340335416	26.78049131212744	22.87206049564087
32-33	23.299252935979762	27.40886482367567	26.122693136699088	23.169189103645483
34-35	23.39819453517182	27.209913982004657	26.7556504021294	22.63624108069412
36-37	23.180211970467727	26.95139063526263	26.53411046363983	23.334286930629812
38-39	23.225952734578687	27.079841641315603	26.93446945639576	22.759736167709953
40-41	23.040458592083258	26.54353764151054	27.281607444830737	23.134396321575466
42-43	22.921093684838112	26.690630280526268	26.88461692919345	23.503659105442164
44-45	23.06039497793327	27.243378591350247	26.823410689532572	22.872815741183906
46-47	23.036040196442013	27.04195505797649	26.546895581265765	23.375109164315727
48-49	23.41093139791782	26.895635542813558	26.628163932793836	23.065269126474785
50-51	23.18933107824751	26.852025067236173	26.629033027336575	23.32961082717975
52-53	22.8881068389395	27.13142656354941	26.54601753335999	23.434449064151103
54-55	22.769002804759502	27.089066405174133	26.678280777197266	23.4636500128691
56-57	23.456316518803174	26.758461132460386	26.420764541227822	23.364457807508618
58-59	22.838520247194648	26.944275688478008	26.962771918990715	23.254432145336633
60-61	22.844900969731864	27.354901599829006	26.57508842981027	23.225109000628862
62-63	23.023318790952178	26.954283290065284	26.34284357456456	23.679554344417976
64-65	22.568722891238767	26.991810150175045	26.63839314120927	23.801073817376913
66-67	22.943372345987985	26.767170954895757	26.5909987219822	23.69845797713406
68-69	22.61975186376518	26.865751690509505	26.71964599490836	23.794850450816952
70-71	22.597849203026872	26.51316410514678	26.856049616896392	24.032937074929954
72-73	22.91843515501534	26.607616145665364	27.089312214030027	23.384636485289263
74-75	23.035866065655462	26.799249596793572	26.790242089425398	23.374642248125568
76-77	22.85226778970298	26.65606819263114	26.647679518551882	23.843984499114
78-79	22.723702344489944	26.657957464225618	26.662381683042042	23.955958508242396
80-81	22.47991342550797	26.89577838155664	26.975950472946153	23.648357719989242
82-83	22.99476611339503	26.288437783318923	27.599083940115104	23.117712163170943
84-85	23.083973691266543	26.625977835522225	26.782110258948023	23.50793821426321
86-87	22.302161483311554	26.974090681437545	27.130167031315782	23.59358080393511
88-89	22.911675410516903	26.200508040957928	27.00821387975055	23.87960266877462
90-91	22.680647950703	26.244903675094694	27.092200502726797	23.982247871475504
92-93	22.809863728885755	26.347281281488005	27.112356351939752	23.730498637686487
94-95	22.13790543582522	26.251087787613653	27.52593821254516	24.08506856401597
96-97	22.344142650380125	26.19840375194834	27.25576400817508	24.201689589496453
98-99	21.94805112509426	26.5551965959346	27.169254866176644	24.327497412794493
100	22.435117400337496	26.16177145530895	26.73136020686295	24.671750937490604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13847.0
1	8167.5
2	2371.0
3	1838.5
4	1090.5
5	668.5
6	542.5
7	488.0
8	488.5
9	484.5
10	459.5
11	464.0
12	448.0
13	421.5
14	468.5
15	599.0
16	678.5
17	768.0
18	998.5
19	1287.0
20	1638.5
21	2175.5
22	2985.0
23	4263.0
24	5822.5
25	7593.5
26	10333.5
27	13894.5
28	18891.5
29	26943.5
30	36759.5
31	45895.0
32	57459.5
33	68311.5
34	77155.0
35	87459.5
36	97128.0
37	111300.5
38	119564.5
39	117942.0
40	115204.0
41	112335.5
42	114412.0
43	121276.5
44	130537.0
45	146317.5
46	161058.0
47	170855.0
48	177514.0
49	176770.5
50	171806.5
51	173519.0
52	174189.0
53	167022.5
54	159063.5
55	143743.5
56	128321.0
57	110439.5
58	92873.0
59	85376.0
60	72211.5
61	55104.5
62	43497.5
63	35414.0
64	26382.5
65	16518.5
66	11094.5
67	8196.0
68	5969.0
69	4382.0
70	3423.0
71	2611.5
72	1908.5
73	1531.0
74	1181.0
75	839.0
76	643.0
77	433.5
78	257.5
79	179.5
80	117.0
81	68.0
82	50.5
83	42.0
84	32.0
85	19.5
86	15.5
87	16.5
88	15.0
89	12.0
90	9.5
91	7.0
92	8.5
93	7.5
94	4.5
95	4.5
96	4.0
97	3.0
98	2.5
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9843814104935624
2	0.4409743569195658
3	0.47376658570214575
4	0.4590420391948254
5	0.42703643059293706
6	0.4774047040378443
7	0.4931371076516758
8	0.4960131876873293
9	0.5097298770881385
10-11	0.5233605299066847
12-13	0.5218241623662715
14-15	0.5188866276290014
16-17	0.5237661309373538
18-19	0.5223895456211436
20-21	0.5216889620227152
22-23	0.4848038501124744
24-25	0.519230773958054
26-27	0.49886468584233623
28-29	0.5054772117362748
30-31	0.5081320548461088
32-33	0.5111433352253187
34-35	0.5227951466518127
36-37	0.5396828986560348
38-39	0.5291741446796083
40-41	0.5343977943170134
42-43	0.531570878042653
44-45	0.541133229614185
46-47	0.5397566442979747
48-49	0.5132327950802807
50-51	0.5280187962892177
52-53	0.5192430648983772
54-55	0.5290635262166986
56-57	0.5177189882982874
58-59	0.522770564771166
60-61	0.5172519325660018
62-63	0.5109589711204692
64-65	0.5225984916066397
66-67	0.5197838662726028
68-69	0.5406415920012526
70-71	0.5248477336858047
72-73	0.5285595976634431
74-75	0.5263595193455713
76-77	0.5140931609029121
78-79	0.5438741093062821
80-81	0.5191078645548209
82-83	0.5196609568693696
84-85	0.5278221412440448
86-87	0.8307077836312697
88-89	1.0949384187016982
90-91	1.0860643597882713
92-93	1.0861135235495647
94-95	1.0911528090821199
96-97	1.084085518396219
98-99	1.0793289244910997
100	1.0749656406763262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4068037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.822235995640504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27132482438033	34.949226458029095
2	12.85329970870579	11.77933865150028
3	4.5559082215792195	6.262857051110456
4	2.15451570611893	3.948989085683826
5	1.1334925635216808	2.5969581872497
6	0.6757226945350864	1.8577874865958097
7	0.45023264642062844	1.4441466604059463
8	0.30290076637363195	1.1103672320026345
9	0.22521451248750513	0.9287849286761027
>10	1.1397307645664074	9.475107232295246
>50	0.10428559460489283	3.351836189992561
>100	0.10962760942989454	10.738517019798437
>500	0.016830107484415817	5.248190469887302
>1k	0.006539086314475853	5.050676585188506
>5k	3.215944089086485E-4	0.956132148087902
>10k+	5.359906815144141E-5	0.30108461349626486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12259	0.30134927484681184	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8699	0.2138377797448745	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7517	0.18478199682057955	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6613	0.16255997671604264	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5753	0.14141955935995668	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5307	0.13045604059156787	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5041	0.1239172603395692	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4445	0.10926645947418866	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.458188064661162E-5	0.0
5	0.0	2.458188064661162E-5	0.0	2.458188064661162E-5	0.0
6	0.0	2.458188064661162E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
7	0.0	2.458188064661162E-5	0.0	7.374564193983486E-5	2.458188064661162E-5
8	0.0	2.458188064661162E-5	0.0	7.374564193983486E-5	2.458188064661162E-5
9	0.0	2.458188064661162E-5	0.0	9.832752258644648E-5	7.374564193983486E-5
10-11	0.0	2.458188064661162E-5	0.0	9.832752258644648E-5	7.374564193983486E-5
12-13	0.0	2.458188064661162E-5	0.0	1.229094032330581E-4	7.374564193983486E-5
14-15	1.229094032330581E-5	2.458188064661162E-5	0.0	1.8436410484958714E-4	9.832752258644648E-5
16-17	2.458188064661162E-5	2.458188064661162E-5	0.0	2.089459854961988E-4	9.832752258644648E-5
18-19	2.458188064661162E-5	2.458188064661162E-5	0.0	2.212369258195046E-4	1.229094032330581E-4
20-21	2.458188064661162E-5	2.458188064661162E-5	0.0	2.335278661428104E-4	1.4749128387966972E-4
22-23	2.458188064661162E-5	2.458188064661162E-5	0.0	2.5810974678942204E-4	2.212369258195046E-4
24-25	2.458188064661162E-5	2.458188064661162E-5	0.0	3.8101915002248013E-4	2.458188064661162E-4
26-27	2.458188064661162E-5	7.374564193983486E-5	0.0	7.251654790750428E-4	2.7040068711272784E-4
28-29	2.458188064661162E-5	7.374564193983486E-5	0.0	0.0017698954065560367	2.8269162743603364E-4
30-31	2.458188064661162E-5	1.229094032330581E-4	0.0	0.006403579908442328	3.0727350808264524E-4
32-33	2.458188064661162E-5	1.7207316452628134E-4	0.0	0.017121279870364995	3.195644484059511E-4
34-35	2.458188064661162E-5	1.9665504517289297E-4	0.0	0.033603430843918085	4.301829113157034E-4
36-37	2.458188064661162E-5	2.9498256775933944E-4	0.0	0.06408496284571649	4.424738516390092E-4
38-39	2.458188064661162E-5	2.9498256775933944E-4	0.0	0.1112575918065642	4.54764791962315E-4
40-41	2.458188064661162E-5	3.318553887292569E-4	0.0	0.16334659689673423	4.670557322856208E-4
42-43	4.916376129322324E-5	3.441463290525627E-4	0.0	0.21865582835161038	4.670557322856208E-4
44-45	4.916376129322324E-5	3.441463290525627E-4	0.0	0.2872761481766267	4.793466726089266E-4
46-47	4.916376129322324E-5	3.441463290525627E-4	0.0	0.3771352128803155	4.916376129322324E-4
48-49	4.916376129322324E-5	3.441463290525627E-4	0.0	0.48115344083645256	5.162194935788441E-4
50-51	4.916376129322324E-5	5.285104339021498E-4	0.0	0.5961351875609784	5.162194935788441E-4
52-53	4.916376129322324E-5	7.12874538751737E-4	0.0	0.7093593298192715	5.776741951953731E-4
54-55	4.916376129322324E-5	7.251654790750428E-4	0.0	0.8348867033411937	6.145470161652906E-4
56-57	4.916376129322324E-5	7.374564193983487E-4	0.0	0.9903670984310122	6.268379564885963E-4
58-59	4.916376129322324E-5	7.620383000449603E-4	0.0	1.1790576142744031	6.391288968119022E-4
60-61	4.916376129322324E-5	8.234930016614893E-4	0.0	1.3811575460105205	6.637107774585138E-4
62-63	4.916376129322324E-5	8.357839419847952E-4	0.0	1.5898945855212232	7.374564193983487E-4
64-65	4.916376129322324E-5	8.603658226314068E-4	0.0	1.81162314895366	7.374564193983487E-4
66-67	4.916376129322324E-5	8.726567629547125E-4	0.0	2.067766345291353	7.497473597216544E-4
68-69	4.916376129322324E-5	8.849477032780184E-4	0.0	2.3588158121472347	7.620383000449603E-4
70-71	4.916376129322324E-5	8.849477032780184E-4	0.0	2.6800272465565085	7.866201806915719E-4
72-73	4.916376129322324E-5	8.849477032780184E-4	0.0	3.0104445952679386	8.357839419847952E-4
74-75	4.916376129322324E-5	8.849477032780184E-4	0.0	3.3415379456971506	8.357839419847952E-4
76-77	4.916376129322324E-5	8.849477032780184E-4	0.0	3.7188821045629625	8.48074882308101E-4
78-79	4.916376129322324E-5	8.849477032780184E-4	0.0	4.133172830040632	8.726567629547125E-4
80-81	4.916376129322324E-5	8.849477032780184E-4	0.0	4.5815832058557975	9.0952958392463E-4
82-83	6.145470161652905E-5	8.849477032780184E-4	0.0	5.059934312298536	0.0010570208678042997
84-85	7.374564193983486E-5	8.849477032780184E-4	0.0	5.526904008001893	0.0010570208678042997
86-87	7.374564193983486E-5	8.849477032780184E-4	0.0	6.023998798437674	0.0010693118081276055
88	7.374564193983486E-5	8.849477032780184E-4	0.0	6.418279873069001	0.0010816027484509114
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6815	0.0	34.405483	1
GTACTAG	2735	0.0	33.291786	1
CAACGCA	6385	0.0	33.03356	5
ATCAACG	6635	0.0	31.511147	3
GGTATCA	4350	0.0	30.150372	1
TAGGCAT	3705	0.0	28.14717	5
AACGCAG	7870	0.0	27.617968	6
TATCAAC	8335	0.0	27.394945	2
TAGTACT	3970	0.0	26.152266	4
GTACATG	14375	0.0	25.960064	1
GCATCGT	3060	0.0	25.92883	8
GGGCACC	3640	0.0	25.686857	94
CTGTGCG	3970	0.0	25.545738	9
ACGCAGA	8270	0.0	25.205488	7
TCTAACG	1420	0.0	25.145773	2
CTAGGCA	4235	0.0	25.070473	4
TACATGG	14330	0.0	25.0488	2
TCAACGC	8560	0.0	25.026413	4
CGCAGAG	8385	0.0	24.803804	8
ACATGGG	14080	0.0	24.793125	3
>>END_MODULE