FastQCFastQC Report
Mon 16 Jan 2017
ERR1146462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4068037
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC98890.2430902177143423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85730.21074046278340142No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT77750.19112412202740536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76410.1878301500207594No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA68800.16912333884868794No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA60840.14955616185398513No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC57820.1421324338987084No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57640.14168996004706938No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC48620.1195171037038257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48120.11828800967149512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.046.5131951
GTATCAA123950.039.9055861
CAACGCA148600.032.1326985
ATCAACG149500.031.8850083
GTACTAG29900.031.2929421
TATCAAC163000.030.0809692
AACGCAG165400.029.5220836
TCAACGC171150.028.0435664
TAGTACT42050.027.9484964
CTGTGCG42600.027.6939249
GGGCACC37100.027.5061994
GTGGTAT24550.026.6212141
ACGCAGA179700.026.5451347
CGCAGAG182300.026.1149838
TAGGCAT40450.025.793295
CTAGTAC45650.025.7447663
GTACTGT49850.025.4545576
GTACATG142650.024.8851181
TCTAACG14350.024.5699622
TACCCCG3500.024.1700065
Produced by FastQC (version 0.11.5)