##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4068037
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.849255550035558	31.0	31.0	34.0	30.0	34.0
2	31.788269379064154	31.0	31.0	34.0	30.0	34.0
3	31.982010488105196	33.0	31.0	34.0	30.0	34.0
4	35.72349759847317	37.0	35.0	37.0	35.0	37.0
5	35.63252374548216	37.0	35.0	37.0	33.0	37.0
6	35.669782501978226	37.0	35.0	37.0	33.0	37.0
7	35.586634290691066	37.0	35.0	37.0	33.0	37.0
8	35.56474019287435	37.0	35.0	37.0	33.0	37.0
9	37.160953796634594	39.0	37.0	39.0	33.0	39.0
10-11	37.111093876481455	39.0	37.0	39.0	33.0	39.0
12-13	37.05983918042043	39.0	37.0	39.0	33.0	39.0
14-15	38.2674458221496	40.0	38.0	41.0	33.0	41.0
16-17	38.25861687098716	40.0	38.0	41.0	33.0	41.0
18-19	38.31542203278879	40.0	38.0	41.0	33.0	41.0
20-21	38.33189484264769	40.0	38.0	41.0	33.0	41.0
22-23	38.29980762220206	40.0	38.0	41.0	33.0	41.0
24-25	38.244064766372574	40.0	37.5	41.0	33.0	41.0
26-27	38.11069183982349	40.0	37.0	41.0	33.0	41.0
28-29	38.04009046623716	40.0	37.0	41.0	33.0	41.0
30-31	37.913238866804804	40.0	37.0	41.0	33.0	41.0
32-33	37.79981327603461	40.0	37.0	41.0	33.0	41.0
34-35	37.70929603147661	40.0	36.5	41.0	32.5	41.0
36-37	37.62250318765537	40.0	36.0	41.0	32.0	41.0
38-39	37.521271438779934	40.0	36.0	41.0	32.0	41.0
40-41	37.40607177368348	40.0	36.0	41.0	31.0	41.0
42-43	37.2885333884623	40.0	36.0	41.0	31.0	41.0
44-45	37.122334064316526	39.0	35.5	41.0	31.0	41.0
46-47	36.91139424248108	39.0	35.0	41.0	30.5	41.0
48-49	36.68801303921277	39.0	35.0	41.0	30.0	41.0
50-51	36.51335005065097	39.0	35.0	41.0	30.0	41.0
52-53	36.34568773587851	39.0	35.0	41.0	29.5	41.0
54-55	36.161512174053485	38.5	35.0	40.5	29.0	41.0
56-57	35.94193673754688	38.0	35.0	40.0	29.0	41.0
58-59	35.699351923298636	38.0	34.0	40.0	28.0	41.0
60-61	35.454090019338565	37.0	34.0	40.0	28.0	41.0
62-63	35.19137706957926	37.0	34.0	40.0	28.0	41.0
64-65	34.927358330320004	36.0	34.0	39.5	28.0	41.0
66-67	34.692614521451006	36.0	34.0	39.0	28.0	41.0
68-69	34.487443452456304	35.5	33.5	39.0	28.0	41.0
70-71	34.233879632854865	35.0	33.0	38.5	27.0	40.5
72-73	33.99771683492554	35.0	33.0	37.5	27.0	40.0
74-75	33.79128889437338	35.0	33.0	37.0	27.0	39.5
76-77	33.5978962334905	35.0	33.0	37.0	27.0	39.0
78-79	33.45057321258386	35.0	33.0	36.0	27.0	39.0
80-81	33.26601773779343	35.0	33.0	36.0	27.0	38.0
82-83	33.06473675141106	35.0	33.0	35.5	27.0	37.0
84-85	32.90099586114876	35.0	33.0	35.0	27.0	37.0
86-87	32.745564138182615	35.0	33.0	35.0	27.0	36.5
88-89	32.61683079578677	35.0	33.0	35.0	27.0	36.0
90-91	32.52070839080372	35.0	33.0	35.0	26.5	36.0
92-93	32.442599096320905	35.0	33.0	35.0	26.0	36.0
94-95	32.38293149742738	35.0	33.0	35.0	26.0	35.0
96-97	32.3225087431604	35.0	33.0	35.0	26.0	35.0
98-99	32.277883780309764	35.0	33.0	35.0	26.0	35.0
100	31.789856631097503	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41075297981886294
1101	2	0.5492528713775897
1101	3	0.4407744499213244
1101	4	0.29835008464371526
1101	5	0.33293940046786474
1101	6	0.36128423667009457
1101	7	0.3746287079485029
1101	8	0.41112750265220654
1101	9	0.4374718668488171
1101	10-11	0.49487428140734124
1101	12-13	0.45703156609651074
1101	14-15	0.6151712365268835
1101	16-17	0.5838102273906145
1101	18-19	0.6006211867818152
1101	20-21	0.5820111523077074
1101	22-23	0.5757269817793755
1101	24-25	0.6196981173844236
1101	26-27	0.5885464220747494
1101	28-29	0.6547665882366474
1101	30-31	0.7364406943352506
1101	32-33	0.7029774860583515
1101	34-35	0.6270412279742743
1101	36-37	0.6361604064878676
1101	38-39	0.6647526190424955
1101	40-41	0.7668987956438542
1101	42-43	0.6936991187618133
1101	44-45	0.7315198744431939
1101	46-47	0.7388604710898008
1101	48-49	0.7259835290030523
1101	50-51	0.6823069161751647
1101	52-53	0.7447610461202459
1101	54-55	0.6313237996191958
1101	56-57	0.6508906880405121
1101	58-59	0.6757404989438243
1101	60-61	0.7348586049992676
1101	62-63	0.6842986443501502
1101	64-65	0.657681245388055
1101	66-67	0.6373723576171386
1101	68-69	0.618123777293043
1101	70-71	0.5395231527464048
1101	72-73	0.541028101674307
1101	74-75	0.5222950094100014
1101	76-77	0.6049049139216436
1101	78-79	0.5551101699921759
1101	80-81	0.5868881775695769
1101	82-83	0.5264697676295214
1101	84-85	0.5307962635553096
1101	86-87	0.5438643947314006
1101	88-89	0.4467931156828726
1101	90-91	0.44530850747133144
1101	92-93	0.48162630766856296
1101	94-95	0.45070515159836333
1101	96-97	0.46013113964036734
1101	98-99	0.49602806350324613
1101	100	0.5762890383129573
1102	1	0.44136216143887097
1102	2	0.565246732378597
1102	3	0.454082498863432
1102	4	0.3013688233679872
1102	5	0.30873334838760513
1102	6	0.38299428416122794
1102	7	0.38378935459127916
1102	8	0.35308510562676076
1102	9	0.4254489817403666
1102	10-11	0.4442489203368112
1102	12-13	0.48704078157641817
1102	14-15	0.6723794988096756
1102	16-17	0.6794426484263454
1102	18-19	0.6833398638950285
1102	20-21	0.6453180522237503
1102	22-23	0.6332788610333111
1102	24-25	0.6348113735381204
1102	26-27	0.6943195071719188
1102	28-29	0.708472357278346
1102	30-31	0.6921826830055622
1102	32-33	0.7070825634736195
1102	34-35	0.7206866338771789
1102	36-37	0.716520598523573
1102	38-39	0.7417620910897398
1102	40-41	0.6503952615887627
1102	42-43	0.7490271235231489
1102	44-45	0.7107203210216255
1102	46-47	0.7286753731724147
1102	48-49	0.6822870859804979
1102	50-51	0.6637680351694684
1102	52-53	0.6711670176814195
1102	54-55	0.7147613452529882
1102	56-57	0.6902568299949721
1102	58-59	0.5909711012022001
1102	60-61	0.661829853749154
1102	62-63	0.611147427284294
1102	64-65	0.6046458385135693
1102	66-67	0.6500187711722702
1102	68-69	0.5615960584210313
1102	70-71	0.5971444099030876
1102	72-73	0.5679121844344408
1102	74-75	0.5492678144356944
1102	76-77	0.5111408238077431
1102	78-79	0.5369490131236461
1102	80-81	0.49451557256168854
1102	82-83	0.46857238060205475
1102	84-85	0.49819883050862046
1102	86-87	0.4647490051708445
1102	88-89	0.46563059801841433
1102	90-91	0.4537648302272217
1102	92-93	0.4853798412873189
1102	94-95	0.5267590412115766
1102	96-97	0.479055307992752
1102	98-99	0.4782040512438641
1102	100	0.5503339940303675
1103	1	0.26475544791492567
1103	2	-0.15980051825796338
1103	3	0.22013845704877966
1103	4	0.2247520840744528
1103	5	0.21459468192049513
1103	6	0.2636704527630158
1103	7	0.3225899943217314
1103	8	0.33149329164364616
1103	9	0.3859360326674448
1103	10-11	0.4485234959445492
1103	12-13	0.4350625869057154
1103	14-15	0.5620518048518122
1103	16-17	0.5153707132897125
1103	18-19	0.5250280931618221
1103	20-21	0.5235327744667657
1103	22-23	0.5110883551013217
1103	24-25	0.49884855427252006
1103	26-27	0.5446307857309662
1103	28-29	0.5855058375923292
1103	30-31	0.6466087724565384
1103	32-33	0.6453508060569533
1103	34-35	0.6486002712263854
1103	36-37	0.6760415538211078
1103	38-39	0.6231697331246764
1103	40-41	0.6622928039800229
1103	42-43	0.6532688395511457
1103	44-45	0.6651993455766103
1103	46-47	0.6911184085724997
1103	48-49	0.6967764097351434
1103	50-51	0.7274393472692751
1103	52-53	0.6992954415536872
1103	54-55	0.7460934342396044
1103	56-57	0.6156273757230366
1103	58-59	0.5472159563993912
1103	60-61	0.611718011252556
1103	62-63	0.5435293918362447
1103	64-65	0.5666769778823166
1103	66-67	0.6240310409873189
1103	68-69	0.600063689096622
1103	70-71	0.5947291588367136
1103	72-73	0.5140661505137629
1103	74-75	0.554528129465524
1103	76-77	0.5094309264063881
1103	78-79	0.49446572593762994
1103	80-81	0.4538115476709095
1103	82-83	0.39669982108544133
1103	84-85	0.44585467126294276
1103	86-87	0.51529886346691
1103	88-89	0.48250262506592634
1103	90-91	0.4923836869249172
1103	92-93	0.43802386218697364
1103	94-95	0.4546774995648164
1103	96-97	0.4433883928405038
1103	98-99	0.5071416602561172
1103	100	0.5258197881384774
1104	1	0.32046483006595494
1104	2	0.3863681643180428
1104	3	0.35294819879444717
1104	4	0.23218915501266935
1104	5	0.2407126943428537
1104	6	0.24614511768618996
1104	7	0.25428826172937846
1104	8	0.28252794349065624
1104	9	0.35505339128136626
1104	10-11	0.3515555325578177
1104	12-13	0.38876641168505444
1104	14-15	0.4950854759861727
1104	16-17	0.43832674467252275
1104	18-19	0.48262289538477887
1104	20-21	0.4341473543948453
1104	22-23	0.4656167957682058
1104	24-25	0.516362447701745
1104	26-27	0.5472836134524428
1104	28-29	0.5565314995733743
1104	30-31	0.5265360362682401
1104	32-33	0.5227625419183397
1104	34-35	0.4996586236291307
1104	36-37	0.5653068365932583
1104	38-39	0.579375192425104
1104	40-41	0.5460513852157192
1104	42-43	0.5094440602114929
1104	44-45	0.5353611631640547
1104	46-47	0.5550821222044036
1104	48-49	0.5856494074468159
1104	50-51	0.5588515734869972
1104	52-53	0.5575939051038716
1104	54-55	0.5211432871327091
1104	56-57	0.5668557183186564
1104	58-59	0.6283078164843872
1104	60-61	0.6401039216808044
1104	62-63	0.6073304706201554
1104	64-65	0.516752983112788
1104	66-67	0.47585925282125174
1104	68-69	0.5553742481028365
1104	70-71	0.5413440656690156
1104	72-73	0.476803156071135
1104	74-75	0.4977939025939975
1104	76-77	0.4721981706038392
1104	78-79	0.47238579785656043
1104	80-81	0.4373265852648984
1104	82-83	0.3946149894411093
1104	84-85	0.38244015671570253
1104	86-87	0.3998828778506862
1104	88-89	0.39305018962951266
1104	90-91	0.41289284024299633
1104	92-93	0.3642350423111935
1104	94-95	0.4057334001859303
1104	96-97	0.37894167822788916
1104	98-99	0.4159084140972453
1104	100	0.5084382909171623
1105	1	0.16903138534303608
1105	2	-0.4060594324516593
1105	3	0.023597739312410937
1105	4	0.056754039445969795
1105	5	0.15401075430758482
1105	6	0.18619269032148367
1105	7	0.26746193665744045
1105	8	0.21890894228393165
1105	9	0.3343305192865671
1105	10-11	0.2847165601267463
1105	12-13	0.28955275253719037
1105	14-15	0.38741699923874506
1105	16-17	0.43398939667288516
1105	18-19	0.4004067067650823
1105	20-21	0.4178070824186051
1105	22-23	0.3516450608754198
1105	24-25	0.4618656733584814
1105	26-27	0.39119211154953604
1105	28-29	0.3900420119654697
1105	30-31	0.4162521088571154
1105	32-33	0.4446611541897596
1105	34-35	0.43681053117578017
1105	36-37	0.44954579045940335
1105	38-39	0.43527338698557827
1105	40-41	0.44649311453902385
1105	42-43	0.3837271057883953
1105	44-45	0.39823362208914403
1105	46-47	0.32871713566398597
1105	48-49	0.3158388857722585
1105	50-51	0.37557695174746186
1105	52-53	0.35441918589845045
1105	54-55	0.38749794693035255
1105	56-57	0.40295963551906055
1105	58-59	0.3681892329886125
1105	60-61	0.30001214130393805
1105	62-63	0.38997713357383645
1105	64-65	0.3463765791398927
1105	66-67	0.38906727287137244
1105	68-69	0.3247569641522148
1105	70-71	0.31059450658612064
1105	72-73	0.2616286457211814
1105	74-75	0.27358581292537565
1105	76-77	0.25295759230624526
1105	78-79	0.2254818481675258
1105	80-81	0.2856457628384561
1105	82-83	0.2661629565811836
1105	84-85	0.3263968076021797
1105	86-87	0.34489494505574214
1105	88-89	0.32236201084922556
1105	90-91	0.24176304817022043
1105	92-93	0.24603177952459987
1105	94-95	0.27400500512420223
1105	96-97	0.20834075462258284
1105	98-99	0.30583136963423385
1105	100	0.2639198188109688
1106	1	0.22683891006232315
1106	2	0.29501103847174903
1106	3	0.22752559250497129
1106	4	0.1508863504900475
1106	5	0.17032269218815088
1106	6	0.16231064462571254
1106	7	0.17596984124377713
1106	8	0.15187846912601088
1106	9	0.19503592680570137
1106	10-11	0.24839491608451425
1106	12-13	0.27696791090822614
1106	14-15	0.2975707615983936
1106	16-17	0.29091114685444097
1106	18-19	0.3033883837663609
1106	20-21	0.35096689192645414
1106	22-23	0.31545644012797425
1106	24-25	0.32087599401095446
1106	26-27	0.3291002591390608
1106	28-29	0.27760590504406224
1106	30-31	0.29417617734491586
1106	32-33	0.3363798430898228
1106	34-35	0.33721064848766247
1106	36-37	0.4041652949563641
1106	38-39	0.40801897382429786
1106	40-41	0.2919588008135321
1106	42-43	0.3880547271503332
1106	44-45	0.41678984799831653
1106	46-47	0.3766446257483338
1106	48-49	0.29801969969326336
1106	50-51	0.30510533005034546
1106	52-53	0.3350169876972302
1106	54-55	0.4093560135188099
1106	56-57	0.3891510494065571
1106	58-59	0.39650818165069524
1106	60-61	0.4384440026524601
1106	62-63	0.33697380973718793
1106	64-65	0.3592906621520129
1106	66-67	0.37556802701918457
1106	68-69	0.3178327699731227
1106	70-71	0.35054897840333155
1106	72-73	0.29699957471955685
1106	74-75	0.24076048193828115
1106	76-77	0.2787560812174732
1106	78-79	0.27684524965347634
1106	80-81	0.2605207698213121
1106	82-83	0.3012360670219394
1106	84-85	0.2725269120656151
1106	86-87	0.24838218040572002
1106	88-89	0.22462131200558844
1106	90-91	0.26627459780431906
1106	92-93	0.2831382959203381
1106	94-95	0.22570323427341776
1106	96-97	0.2629183620188158
1106	98-99	0.27505934572495505
1106	100	0.31653908589102286
1107	1	0.04981080547140593
1107	2	-0.5180113051956035
1107	3	-0.05093697638103478
1107	4	0.06485857535135864
1107	5	0.07039170226693159
1107	6	0.11586898862960737
1107	7	0.09813578401362122
1107	8	0.0960721927066075
1107	9	0.15544957132721748
1107	10-11	0.17703414604547163
1107	12-13	0.13517781782498162
1107	14-15	0.182831910389325
1107	16-17	0.24594245426929717
1107	18-19	0.24970962662843021
1107	20-21	0.18198210108607782
1107	22-23	0.13478847928599436
1107	24-25	0.19694729276645973
1107	26-27	0.2415729328022138
1107	28-29	0.1508737638834745
1107	30-31	0.20035073597582453
1107	32-33	0.23369771438413522
1107	34-35	0.24334351746824723
1107	36-37	0.2950409572286006
1107	38-39	0.2638112355256439
1107	40-41	0.2035735752096599
1107	42-43	0.2434974717095173
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2216	82-83	-0.7124613597062393
2216	84-85	-0.7276502212094584
2216	86-87	-0.6885662231024909
2216	88-89	-0.7121459293138379
2216	90-91	-0.6866215659751873
2216	92-93	-0.6927753528815757
2216	94-95	-0.6859583406798961
2216	96-97	-0.7447757285835834
2216	98-99	-0.7046840634121594
2216	100	-0.6959190562425697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	15.0
13	70.0
14	221.0
15	657.0
16	1695.0
17	3147.0
18	6024.0
19	9148.0
20	13076.0
21	18685.0
22	25191.0
23	30561.0
24	32846.0
25	35813.0
26	40052.0
27	49044.0
28	61614.0
29	77238.0
30	95778.0
31	118001.0
32	147230.0
33	184605.0
34	247099.0
35	338789.0
36	506195.0
37	856375.0
38	869343.0
39	298754.0
40	769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96850494170045	20.21213381005215	13.826214306629064	24.993146941618335
2	15.051968612334802	20.137861862806798	39.74563896318439	25.064530561674008
3	18.261447143751237	25.40363002764532	29.70337943508758	26.631543393515862
4	12.492737209559074	16.91923432792479	37.65352672251998	32.93450173999616
5	12.886128207630588	38.11692814774736	35.08058301091197	13.91636063371008
6	30.99824313299019	37.97996429236017	17.237650493346056	13.784142081303589
7	26.942922137589225	32.060937723542295	22.830260013343093	18.165880125525376
8	25.97591909808097	35.442032606881405	19.559089555970115	19.022958739067516
9	25.817931351165193	15.022257663831477	20.678867965065216	38.48094301993812
10-11	25.33810533188366	25.96523827094001	27.61482995361153	21.081826443564797
12-13	25.885801923630485	23.587887720785233	27.950151387511962	22.576158968072317
14-15	22.985611913153498	24.82845673070482	25.708989994482145	26.476941361659538
16-17	22.052576669290445	27.512090927907735	26.792227783887906	23.643104618913913
18-19	21.66921048161558	27.02640118563327	29.17622184852301	22.128166484228142
20-21	22.83092064015697	26.245568036743062	28.7983247925564	22.125186530543566
22-23	22.92671151419147	25.96832453049479	28.604050731488595	22.500913223825137
24-25	22.583020088438243	26.041396137915672	29.04762531014655	22.327958463499527
26-27	22.515731820004234	26.27445625387626	28.451653122433495	22.75815880368601
28-29	22.0461367485104	26.627707663425877	28.794256296095632	22.53189929196809
30-31	22.597618949876807	26.324256637783776	28.67566101291606	22.402463399423358
32-33	22.800861939062994	26.49004913180485	28.115661189905598	22.59342773922656
34-35	22.844965277286992	26.395087824785286	28.625967286989756	22.133979610937963
36-37	22.680177196028453	26.093248414407245	28.39611586620279	22.830458523361514
38-39	22.77352688778396	26.173790946345864	28.74313827529101	22.309543890579167
40-41	22.535443238520177	25.762567205630948	29.023651195293237	22.678338360555635
42-43	22.603774109013838	25.835910045866445	28.473123137831763	23.087192707287954
44-45	22.77426434420336	26.432564403912746	28.213878094029134	22.57929315785476
46-47	22.848538496577095	26.350829650762762	27.689989053688546	23.110642798971593
48-49	23.311970733623514	26.351120313528988	27.522638201991978	22.814270750855517
50-51	22.99850025946175	26.382945877827563	27.36454953580806	23.25400432690263
52-53	22.673785907060335	26.674290327251203	27.288886507177786	23.363037258510676
54-55	22.618433215860144	26.66548755100915	27.275194409664355	23.44088482346635
56-57	23.287999384469103	26.33807297040117	27.05462320487567	23.319304440254047
58-59	22.60120874290934	26.493463171836574	27.62950500832344	23.275823076930642
60-61	22.651830824298045	26.891154467603616	27.232216273671465	23.224798434426877
62-63	22.860878600661692	26.462947608391957	27.01993615102321	23.656237639923138
64-65	22.402709218229823	26.464188993364612	27.31258344012112	23.82051834828444
66-67	22.7070451915752	26.332024512068102	27.253255071180522	23.70767522517617
68-69	22.50413534583143	26.303282293246845	27.37424406566504	23.818338295256687
70-71	22.445963017216563	26.02388740809061	27.467274467238333	24.062875107454495
72-73	22.799227243999994	26.100205086630236	27.631066285778623	23.46950138359115
74-75	22.90859636519173	26.41643892525309	27.227618299040508	23.447346410514676
76-77	22.73770914542876	26.30885589525662	26.983487642219384	23.969947317095237
78-79	22.538192091659297	26.393665815188044	27.071353973915823	23.996788119236832
80-81	22.31022829709966	26.731513377537947	27.30491720422891	23.65334112113348
82-83	22.84893658889611	26.06712610133235	27.90350864147122	23.180428668300323
84-85	22.977632168299078	26.419819430916814	27.05004860113781	23.552499799646295
86-87	22.14114365562299	26.804717522902223	27.338123394681258	23.716015426793533
88-89	22.702408494965372	26.102243341305847	27.167113230558492	24.028234933170296
90-91	22.420078600351765	26.18160189301645	27.254775838547364	24.14354366808443
92-93	22.619471985328282	26.34679633895405	27.092403796749508	23.94132787896816
94-95	21.952240272095196	26.289998888885613	27.48908059709124	24.26868024192795
96-97	22.125590361872284	26.168456804144896	27.26400029106328	24.441952542919545
98-99	21.720682962407764	26.602362440386194	27.15122551149478	24.52572908571126
100	22.12549880189139	26.248001102161517	26.68615950835724	24.94034058758986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	209.0
2	194.0
3	261.0
4	190.0
5	143.0
6	347.5
7	926.5
8	1583.0
9	1777.0
10	1405.5
11	990.5
12	941.5
13	1038.5
14	1176.0
15	1473.5
16	1899.5
17	2377.0
18	3003.0
19	3757.0
20	4579.0
21	5520.5
22	6644.5
23	7988.5
24	9517.5
25	11391.5
26	13910.5
27	16987.5
28	21517.0
29	29161.0
30	38611.0
31	47033.0
32	57615.5
33	67867.5
34	75881.5
35	85570.0
36	95294.0
37	109832.0
38	117783.5
39	116103.5
40	114043.5
41	111196.0
42	113364.0
43	120367.5
44	128524.0
45	144309.5
46	160318.0
47	170797.0
48	178114.5
49	176613.0
50	171344.5
51	173880.5
52	173575.5
53	165742.0
54	159798.5
55	144288.0
56	127980.5
57	109805.0
58	91990.0
59	85860.0
60	72392.0
61	54526.0
62	42635.5
63	34223.0
64	25122.0
65	15375.5
66	10299.5
67	7566.5
68	5566.5
69	4085.0
70	3149.0
71	2391.5
72	1753.5
73	1389.5
74	1038.5
75	717.5
76	511.0
77	334.5
78	191.0
79	105.5
80	50.0
81	24.5
82	16.5
83	9.0
84	2.5
85	0.5
86	0.5
87	2.0
88	2.5
89	1.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012536759129771927
2	0.00484263048738249
3	0.001941968571082318
4	0.02640093981446088
5	0.0029498256775933947
6	0.0
7	3.6872820969917434E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008148893434351753
16-17	0.00272858875177389
18-19	0.0
20-21	8.603658226314068E-5
22-23	7.620383000449603E-4
24-25	1.229094032330581E-5
26-27	1.9665504517289297E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.530923145487615E-4
36-37	0.0
38-39	0.0
40-41	4.54764791962315E-4
42-43	7.497473597216544E-4
44-45	0.0
46-47	0.0
48-49	5.899651355186789E-4
50-51	0.0
52-53	0.0
54-55	4.056010306690918E-4
56-57	9.832752258644648E-5
58-59	2.2123692581950456E-4
60-61	1.4749128387966972E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001425749077503474
70-71	8.849477032780183E-4
72-73	0.0
74-75	2.335278661428104E-4
76-77	0.002802334393713725
78-79	3.8101915002248013E-4
80-81	0.0033431357679391807
82-83	0.0016346950629996728
84-85	0.0056784144293672845
86-87	0.007595801119802991
88-89	0.007620383000449603
90-91	0.005985687937449929
92-93	0.008529912584374232
94-95	0.0012045121516839695
96-97	0.004744302964796043
98-99	0.0020280051533454586
100	0.08104646049187852
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4068037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.132416495536205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.91560164204655	27.359664429925605
2	16.02758550490456	12.543963027914886
3	5.938533322397708	6.971674780340625
4	2.7274050752419243	4.2691980542562495
5	1.4242867804994308	2.786789175179504
6	0.8727429370519291	2.0491524063752173
7	0.5817761154572966	1.5936413680061063
8	0.402197176903955	1.2591157951947538
9	0.31148790163497936	1.0970346870090562
>10	1.4914841396317282	10.645216714036252
>50	0.1330489569098431	3.673069014256231
>100	0.14134330404176831	11.909740449868288
>500	0.022069646608245695	5.861547182952562
>1k	0.009934484782454244	6.543551015197374
>5k	5.030118877192022E-4	1.4366418994872747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	9889	0.2430902177143423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8573	0.21074046278340142	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7775	0.19112412202740536	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7641	0.1878301500207594	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6880	0.16912333884868794	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6084	0.14955616185398513	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5782	0.1421324338987084	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5764	0.14168996004706938	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4862	0.1195171037038257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4812	0.11828800967149512	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.458188064661162E-5	0.0
5	0.0	7.374564193983486E-5	0.0	2.458188064661162E-5	0.0
6	0.0	7.374564193983486E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
7	0.0	9.832752258644648E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
8	0.0	9.832752258644648E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
9	0.0	9.832752258644648E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
10-11	0.0	9.832752258644648E-5	0.0	2.458188064661162E-5	2.458188064661162E-5
12-13	0.0	9.832752258644648E-5	0.0	4.916376129322324E-5	2.458188064661162E-5
14-15	0.0	9.832752258644648E-5	0.0	7.374564193983486E-5	4.916376129322324E-5
16-17	0.0	9.832752258644648E-5	0.0	8.603658226314067E-5	8.603658226314067E-5
18-19	0.0	9.832752258644648E-5	0.0	9.832752258644648E-5	9.832752258644648E-5
20-21	0.0	9.832752258644648E-5	0.0	1.106184629097523E-4	1.4749128387966972E-4
22-23	0.0	1.229094032330581E-4	0.0	1.352003435563639E-4	1.9665504517289297E-4
24-25	0.0	1.5978222420297554E-4	0.0	2.5810974678942204E-4	2.7040068711272784E-4
26-27	2.458188064661162E-5	2.089459854961988E-4	0.0	5.653832548720673E-4	2.949825677593395E-4
28-29	2.458188064661162E-5	2.212369258195046E-4	0.0	0.0016346950629996728	3.318553887292569E-4
30-31	2.458188064661162E-5	2.335278661428104E-4	0.0	0.006268379564885964	3.5643726937586854E-4
32-33	2.458188064661162E-5	2.458188064661162E-4	0.0	0.017096697989718385	3.6872820969917434E-4
34-35	2.458188064661162E-5	2.458188064661162E-4	0.0	0.03377550400844437	3.6872820969917434E-4
36-37	2.458188064661162E-5	3.441463290525627E-4	0.0	0.06449056387638559	3.9331009034578593E-4
38-39	2.458188064661162E-5	3.441463290525627E-4	0.0	0.11246210395824816	3.9331009034578593E-4
40-41	3.687282096991743E-5	3.441463290525627E-4	0.0	0.16570645743880894	4.178919709923976E-4
42-43	4.916376129322324E-5	3.441463290525627E-4	0.0	0.22235540138892543	4.178919709923976E-4
44-45	4.916376129322324E-5	3.5643726937586854E-4	0.0	0.2923400155898287	4.178919709923976E-4
46-47	4.916376129322324E-5	3.9331009034578593E-4	0.0	0.38377232065490063	4.424738516390092E-4
48-49	1.352003435563639E-4	3.9331009034578593E-4	0.0	0.4895604440175938	4.670557322856208E-4
50-51	1.4749128387966972E-4	5.039285532555382E-4	0.0	0.6056729572518638	4.670557322856208E-4
52-53	1.4749128387966972E-4	6.145470161652906E-4	0.0	0.7208267771409158	4.916376129322324E-4
54-55	1.4749128387966972E-4	6.268379564885963E-4	0.0	0.8480503004274543	5.162194935788441E-4
56-57	1.4749128387966972E-4	6.51419837135208E-4	0.0	1.0053374637447987	5.408013742254557E-4
58-59	1.4749128387966972E-4	6.637107774585138E-4	0.0	1.1964247129512344	5.653832548720673E-4
60-61	1.7207316452628134E-4	7.743292403682661E-4	0.0	1.4008107595874866	5.653832548720673E-4
62-63	1.9665504517289297E-4	8.849477032780184E-4	0.0	1.6115020586095947	6.145470161652906E-4
64-65	1.9665504517289297E-4	9.832752258644648E-4	0.0	1.836069829256715	6.145470161652906E-4
66-67	1.9665504517289297E-4	9.955661661877706E-4	0.0	2.0956790707655806	6.268379564885963E-4
68-69	1.9665504517289297E-4	0.0010078571065110765	0.0	2.389862727403905	6.391288968119022E-4
70-71	1.9665504517289297E-4	0.0010078571065110765	0.0	2.7151916268214866	7.12874538751737E-4
72-73	1.9665504517289297E-4	0.0010078571065110765	0.0	3.051275099021961	8.972386436013242E-4
74-75	1.9665504517289297E-4	0.0010078571065110765	0.0	3.387518353446638	9.0952958392463E-4
76-77	1.9665504517289297E-4	0.0010201480468343823	0.0	3.7714135835047715	9.0952958392463E-4
78-79	1.9665504517289297E-4	0.0010324389871576882	0.0	4.196213062958867	9.0952958392463E-4
80-81	1.9665504517289297E-4	0.0010324389871576882	0.0	4.655341138735956	9.218205242479357E-4
82-83	1.9665504517289297E-4	0.0010324389871576882	0.0	5.144041216930917	9.832752258644648E-4
84-85	1.9665504517289297E-4	0.0010324389871576882	0.0	5.621691739775228	9.832752258644648E-4
86-87	1.9665504517289297E-4	0.0010324389871576882	0.0	6.1279309898115475	9.955661661877706E-4
88	1.9665504517289297E-4	0.0010324389871576882	0.0	6.53044699445949	0.0010324389871576882
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6985	0.0	46.513195	1
GTATCAA	12395	0.0	39.905586	1
CAACGCA	14860	0.0	32.132698	5
ATCAACG	14950	0.0	31.885008	3
GTACTAG	2990	0.0	31.292942	1
TATCAAC	16300	0.0	30.080969	2
AACGCAG	16540	0.0	29.522083	6
TCAACGC	17115	0.0	28.043566	4
TAGTACT	4205	0.0	27.948496	4
CTGTGCG	4260	0.0	27.693924	9
GGGCACC	3710	0.0	27.50619	94
GTGGTAT	2455	0.0	26.621214	1
ACGCAGA	17970	0.0	26.545134	7
CGCAGAG	18230	0.0	26.114983	8
TAGGCAT	4045	0.0	25.79329	5
CTAGTAC	4565	0.0	25.744766	3
GTACTGT	4985	0.0	25.454557	6
GTACATG	14265	0.0	24.885118	1
TCTAACG	1435	0.0	24.569962	2
TACCCCG	350	0.0	24.170006	5
>>END_MODULE