FastQCFastQC Report
Mon 16 Jan 2017
ERR1146461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691298
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80940.3007470744599817No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA71020.26388753679451327No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC58630.21785027150467914No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC55490.20618303881621433No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA52490.19503600121577022No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC49230.18292288702328766No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC43530.16174351558244385No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT39660.14736383707787098No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC34510.1282280891971086No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC32420.12046231966879922No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA31800.11815859856470744No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA29100.10812626472430774No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG28300.10515372136418932No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT27730.10303578422010495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62700.031.0790441
GTACTAG17750.029.7717781
CAACGCA66600.028.6394655
CGTTAAC5750.027.8994851
TAGTACT22750.027.879294
GGTATCA42650.027.5464361
GGGCACC21450.027.29643494
ATCAACG69900.027.2227233
GTACATG117850.026.9044911
TACATGG118100.026.5774172
CTAACGC10450.026.5270793
CTGTGCG24450.026.1168889
ACATGGG119400.025.9712773
TCTAACG10800.025.6693272
TATCAAC76400.025.6465472
TATTCTC17050.025.3499165
AACGCAG78800.025.3183166
ATTTAGG32050.025.0268421
ACGCAGA76200.024.5827587
AGGGCTA31250.024.5047725
Produced by FastQC (version 0.11.5)