##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146461_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2691298
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.41718457041918	31.0	30.0	34.0	26.0	34.0
2	30.453403524990545	31.0	30.0	34.0	26.0	34.0
3	30.689847055212763	31.0	31.0	34.0	27.0	34.0
4	34.14952747707612	37.0	35.0	37.0	30.0	37.0
5	34.37442639202348	37.0	35.0	37.0	30.0	37.0
6	34.57730656359868	37.0	35.0	37.0	31.0	37.0
7	34.421053707170294	37.0	35.0	37.0	30.0	37.0
8	34.36904460226998	37.0	35.0	37.0	30.0	37.0
9	35.74568442439299	39.0	35.0	39.0	30.0	39.0
10-11	35.66037391622927	39.0	35.0	39.0	29.5	39.0
12-13	35.66081868302953	39.0	35.0	39.0	30.0	39.0
14-15	36.667793198672165	40.0	36.0	41.0	29.5	41.0
16-17	36.505775465964746	40.0	36.0	41.0	28.0	41.0
18-19	36.453609002050314	39.5	36.0	41.0	27.5	41.0
20-21	36.80829008902025	40.0	36.0	41.0	30.0	41.0
22-23	36.53299040091436	39.5	36.0	41.0	28.0	41.0
24-25	36.659028840358815	39.5	36.0	41.0	29.5	41.0
26-27	36.746511163015015	40.0	36.0	41.0	30.0	41.0
28-29	36.580890521971185	39.5	35.5	41.0	29.5	41.0
30-31	36.521524743822496	39.0	35.0	41.0	29.0	41.0
32-33	36.621636288512086	39.0	35.0	41.0	30.0	41.0
34-35	36.66594632032573	39.0	35.0	41.0	30.0	41.0
36-37	36.5878953203993	39.0	35.0	41.0	30.0	41.0
38-39	36.421579475777115	39.0	35.0	41.0	29.0	41.0
40-41	36.3813485165894	39.0	35.0	41.0	29.0	41.0
42-43	36.21390552068185	39.0	35.0	41.0	28.0	41.0
44-45	36.035518177474216	39.0	35.0	41.0	28.0	41.0
46-47	35.911516859151234	38.5	34.5	40.5	27.5	41.0
48-49	35.79054270467262	38.0	34.0	40.0	27.5	41.0
50-51	35.576845447809944	38.0	34.0	40.0	27.0	41.0
52-53	35.40090172102829	38.0	34.0	40.0	27.0	41.0
54-55	35.211683172952235	38.0	33.5	40.0	26.5	41.0
56-57	35.07205110693799	37.5	33.0	40.0	26.5	41.0
58-59	34.832627601997245	37.0	33.0	40.0	26.0	41.0
60-61	34.56290997875375	36.5	33.0	40.0	26.0	41.0
62-63	34.29645676547153	36.0	33.0	39.5	26.0	41.0
64-65	34.01946532862581	35.5	32.5	39.0	26.0	41.0
66-67	33.71886390879048	35.0	32.0	39.0	25.0	41.0
68-69	33.42963469671511	35.0	32.0	38.5	25.0	40.0
70-71	33.3382897397464	35.0	32.0	38.0	25.0	40.0
72-73	33.10973348176233	35.0	32.0	37.0	25.0	40.0
74-75	32.830455044368925	35.0	32.0	37.0	25.0	39.0
76-77	32.58670853246277	35.0	31.0	36.0	24.5	39.0
78-79	32.35692554299078	35.0	31.0	36.0	24.0	39.0
80-81	32.107667564127055	35.0	31.0	35.5	24.0	37.5
82-83	31.87482359812997	35.0	31.0	35.0	24.0	37.0
84-85	31.66441843303863	34.0	31.0	35.0	23.5	37.0
86-87	31.48349643926462	34.5	31.0	35.0	23.0	36.0
88-89	31.468255094753538	35.0	31.0	35.0	23.0	36.0
90-91	31.373412568953718	35.0	31.0	35.0	23.0	36.0
92-93	31.222045273321648	34.5	31.0	35.0	23.0	36.0
94-95	31.17122314214182	34.0	31.0	35.0	23.0	35.0
96-97	31.067053889981715	34.0	31.0	35.0	21.5	35.0
98-99	30.968460386029342	34.0	31.0	35.0	20.5	35.0
100	30.45682009201508	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.834135209880138
1101	2	0.9410371859823563
1101	3	0.9395026910923399
1101	4	0.9180318343996916
1101	5	0.8562747526325936
1101	6	0.6525621930234351
1101	7	0.7023378865343872
1101	8	0.7458271637104659
1101	9	1.0401104343684011
1101	10-11	0.989619370116003
1101	12-13	0.9110877363815675
1101	14-15	1.1555352474112723
1101	16-17	1.2170930016093422
1101	18-19	1.3470933497808275
1101	20-21	1.095527219776855
1101	22-23	1.453144149364519
1101	24-25	1.2474690515705902
1101	26-27	1.1302576573404721
1101	28-29	1.3076927714951267
1101	30-31	1.34026348169278
1101	32-33	1.166651196844164
1101	34-35	1.11017380065082
1101	36-37	1.031043473234007
1101	38-39	1.078959598697196
1101	40-41	1.1084411578497182
1101	42-43	1.0802798727934473
1101	44-45	1.140382146362228
1101	46-47	1.1622743643545093
1101	48-49	1.103096065374487
1101	50-51	1.08529423993091
1101	52-53	1.0901634221261105
1101	54-55	1.1148994768961202
1101	56-57	0.9132648030664257
1101	58-59	0.860784998718465
1101	60-61	1.0486648297993568
1101	62-63	0.8679071116160628
1101	64-65	0.8630235286288297
1101	66-67	0.8725198860843193
1101	68-69	0.9361442416333929
1101	70-71	0.8714020057811922
1101	72-73	0.8389923482984258
1101	74-75	0.8269345695161832
1101	76-77	0.8587881408452276
1101	78-79	0.7752256391586627
1101	80-81	0.759320064442619
1101	82-83	0.8809243862133123
1101	84-85	0.8540568368333901
1101	86-87	0.8744788299717712
1101	88-89	0.8433646900724447
1101	90-91	0.886567333867788
1101	92-93	0.9074009253770399
1101	94-95	0.8438741994429186
1101	96-97	0.8090668105730501
1101	98-99	0.8440702174944583
1101	100	0.9007928454112708
1102	1	0.7413104760857507
1102	2	0.9298134543164416
1102	3	0.9312257222043456
1102	4	1.08330807787069
1102	5	0.8731856706458458
1102	6	0.6481886061971665
1102	7	0.6610520069126622
1102	8	0.6744289224566629
1102	9	0.7383751130935039
1102	10-11	0.8915194580630654
1102	12-13	0.8083421451910979
1102	14-15	1.0900099952947429
1102	16-17	1.221138249494274
1102	18-19	1.2835512941206773
1102	20-21	1.0143925832604879
1102	22-23	1.317079712055083
1102	24-25	1.1427787163869851
1102	26-27	0.961094972829585
1102	28-29	1.2067910094338572
1102	30-31	1.2698181424482087
1102	32-33	1.0837526830714737
1102	34-35	1.00831621151179
1102	36-37	0.9915967304440585
1102	38-39	1.0376617750456774
1102	40-41	0.9367699833274514
1102	42-43	1.0665960366245812
1102	44-45	1.0539324775287398
1102	46-47	1.059569906940098
1102	48-49	0.9916820336257928
1102	50-51	1.0417527059079745
1102	52-53	0.9879620155581676
1102	54-55	1.0237037625115093
1102	56-57	0.9958577690566557
1102	58-59	0.9189913028261358
1102	60-61	1.0331992003294843
1102	62-63	0.9356555986243791
1102	64-65	0.8100074539147855
1102	66-67	0.9641978711411241
1102	68-69	0.8563534303461395
1102	70-71	0.8942171136625987
1102	72-73	0.8251540928872956
1102	74-75	0.8469948722412752
1102	76-77	0.9153272777745087
1102	78-79	0.7612970722569798
1102	80-81	0.9339604882070489
1102	82-83	0.9526898454442012
1102	84-85	0.7651546808628886
1102	86-87	0.8254184229192418
1102	88-89	0.839451557782322
1102	90-91	0.8896390214470742
1102	92-93	1.0204448296804927
1102	94-95	0.9776565957955619
1102	96-97	0.9463584360043775
1102	98-99	1.0124215611246825
1102	100	1.037117613818971
1103	1	0.6322150486503624
1103	2	0.7874178280738953
1103	3	0.8193966963594121
1103	4	0.8290834257832174
1103	5	0.7730365652780549
1103	6	0.5597194213065109
1103	7	0.6240241739106693
1103	8	0.6195084453194895
1103	9	0.7801246160756108
1103	10-11	0.8199488815223077
1103	12-13	0.7406234573183355
1103	14-15	0.9751988620939471
1103	16-17	1.2200199592358274
1103	18-19	1.2232878746375704
1103	20-21	0.9118826429026399
1103	22-23	1.0763987975421543
1103	24-25	1.0541852770231657
1103	26-27	0.995637716368357
1103	28-29	1.166035542237033
1103	30-31	1.1690477647146125
1103	32-33	0.9918043729323074
1103	34-35	0.9044394719491677
1103	36-37	0.8802647224917735
1103	38-39	1.0343069042703377
1103	40-41	0.9093097447588647
1103	42-43	0.9516606489506287
1103	44-45	1.0252296942285355
1103	46-47	0.9415281574371193
1103	48-49	0.8638241807430873
1103	50-51	0.9480648331445565
1103	52-53	1.0238715583017068
1103	54-55	1.044702768475723
1103	56-57	0.9172046875457198
1103	58-59	0.8591006467625206
1103	60-61	0.8526574508264986
1103	62-63	0.7592142467357093
1103	64-65	0.7600571557040467
1103	66-67	0.8690189446997749
1103	68-69	0.8209409054952417
1103	70-71	0.7103204933842662
1103	72-73	0.7886232777779512
1103	74-75	0.7723179338714985
1103	76-77	0.7806354288679529
1103	78-79	0.7335883247420654
1103	80-81	0.8120668500360679
1103	82-83	0.7393449150267024
1103	84-85	0.6702242633678601
1103	86-87	0.7321033135753012
1103	88-89	0.7165638838260655
1103	90-91	0.6907076099162595
1103	92-93	0.7975323048296019
1103	94-95	0.7814587826742851
1103	96-97	0.7706626109546733
1103	98-99	0.8173420382618559
1103	100	0.8032706953299034
1104	1	0.5245246708407372
1104	2	0.7209457237970724
1104	3	0.6020022105020928
1104	4	0.7074784476215967
1104	5	0.7038897824948336
1104	6	0.45698259180550593
1104	7	0.44195549611131213
1104	8	0.5263955167722258
1104	9	0.5924499945884705
1104	10-11	0.6243873083228806
1104	12-13	0.5326421387289102
1104	14-15	0.6610474417441523
1104	16-17	0.8644290781361903
1104	18-19	0.8190255898377714
1104	20-21	0.7122394409290109
1104	22-23	0.9677388547134669
1104	24-25	0.8295455437212595
1104	26-27	0.7051210173274214
1104	28-29	0.8461463211581517
1104	30-31	0.8630223018132455
1104	32-33	0.7314919644007105
1104	34-35	0.6093051630796325
1104	36-37	0.5680308123769393
1104	38-39	0.6483701348296194
1104	40-41	0.6050079266046922
1104	42-43	0.5570377078211166
1104	44-45	0.5895075072737868
1104	46-47	0.5760551256469739
1104	48-49	0.5732202897487113
1104	50-51	0.742752015974915
1104	52-53	0.6971290971983422
1104	54-55	0.7027694258925976
1104	56-57	0.573953962450787
1104	58-59	0.5786407198992265
1104	60-61	0.6199494920780353
1104	62-63	0.4844590969461109
1104	64-65	0.5708420638460154
1104	66-67	0.6248935916555141
1104	68-69	0.5765525511091312
1104	70-71	0.5297138844717821
1104	72-73	0.5104374691117854
1104	74-75	0.48466241383682984
1104	76-77	0.5655129229854623
1104	78-79	0.4128758880809684
1104	80-81	0.5205009965907763
1104	82-83	0.394193072734371
1104	84-85	0.47178345506281616
1104	86-87	0.3269451136353645
1104	88-89	0.286769561804082
1104	90-91	0.36047937131726115
1104	92-93	0.3337951294117474
1104	94-95	0.23092593767924896
1104	96-97	0.38767349973290166
1104	98-99	0.394689616273709
1104	100	0.44970708654285474
1105	1	0.5609266583229875
1105	2	0.7259251883431084
1105	3	0.6628098211927842
1105	4	0.7786265629067373
1105	5	0.6564328643631114
1105	6	0.3567935052028517
1105	7	0.3407932624199148
1105	8	0.42777205011541497
1105	9	0.5928124180874832
1105	10-11	0.6068054875847295
1105	12-13	0.583386610897449
1105	14-15	0.8109552699114744
1105	16-17	0.8820471186924692
1105	18-19	0.8791609088808556
1105	20-21	0.6572002728289519
1105	22-23	0.9705948690508777
1105	24-25	0.8626072528280133
1105	26-27	0.6515683826651752
1105	28-29	0.8721136212529217
1105	30-31	0.9612269310552435
1105	32-33	0.8187395916928111
1105	34-35	0.6720878241175612
1105	36-37	0.6733412855035255
1105	38-39	0.7811689150948524
1105	40-41	0.5794229624594962
1105	42-43	0.6134522432453409
1105	44-45	0.6125787146476185
1105	46-47	0.6155051826298177
1105	48-49	0.6879960772452947
1105	50-51	0.7240078376206469
1105	52-53	0.7456510920349615
1105	54-55	0.6265321726774218
1105	56-57	0.6429113420331305
1105	58-59	0.5942647270931687
1105	60-61	0.6072954254768348
1105	62-63	0.6420174745055505
1105	64-65	0.5509214736849231
1105	66-67	0.6881878928304559
1105	68-69	0.6454340831252026
1105	70-71	0.5276208803155669
1105	72-73	0.42846236018194617
1105	74-75	0.4774799619387764
1105	76-77	0.5070356886338914
1105	78-79	0.5557225415950882
1105	80-81	0.628530547230703
1105	82-83	0.602293812497404
1105	84-85	0.49937887259676117
1105	86-87	0.5744755472953003
1105	88-89	0.5411421331610704
1105	90-91	0.5065449977973131
1105	92-93	0.5830709583667826
1105	94-95	0.4913691589741127
1105	96-97	0.526223401291606
1105	98-99	0.6641592612496439
1105	100	0.7452635063169168
1106	1	0.46816123994871006
1106	2	0.6122204423216786
1106	3	0.5031796042504837
1106	4	0.5750665357032929
1106	5	0.5019609219028425
1106	6	0.36055004291527837
1106	7	0.2809394506013376
1106	8	0.36979959641103477
1106	9	0.40003693434900356
1106	10-11	0.3855210111847498
1106	12-13	0.3040717712479122
1106	14-15	0.5703989911575036
1106	16-17	0.6558185376414656
1106	18-19	0.6203288888004508
1106	20-21	0.5418355280701874
1106	22-23	0.7524669122097265
1106	24-25	0.6616126858779552
1106	26-27	0.518338262377334
1106	28-29	0.7248510239890393
1106	30-31	0.7245699699010331
1106	32-33	0.5813400852551354
1106	34-35	0.5922212654588392
1106	36-37	0.5634622484892731
1106	38-39	0.6247462747206498
1106	40-41	0.591634883535356
1106	42-43	0.5206972160115626
1106	44-45	0.6414019410147418
1106	46-47	0.6321502731697848
1106	48-49	0.6240593616500192
1106	50-51	0.6330818757885908
1106	52-53	0.5240003138901983
1106	54-55	0.6374016988212006
1106	56-57	0.4866998945686021
1106	58-59	0.5212455087630659
1106	60-61	0.5294868462366935
1106	62-63	0.5076338814536143
1106	64-65	0.534967393988353
1106	66-67	0.534554094146273
1106	68-69	0.4567821971163468
1106	70-71	0.5396383185590494
1106	72-73	0.515967979823067
1106	74-75	0.504046485346251
1106	76-77	0.45010265790554627
1106	78-79	0.4632474292191162
1106	80-81	0.49980102648358127
1106	82-83	0.442175240035656
1106	84-85	0.42604598224430745
1106	86-87	0.39388642804387786
1106	88-89	0.45380973664054025
1106	90-91	0.391318174365594
1106	92-93	0.46547270816743946
1106	94-95	0.47180396136382896
1106	96-97	0.47220076051299387
1106	98-99	0.43670427286188485
1106	100	0.421494384652501
1107	1	0.5373070601912922
1107	2	0.7078196848163039
1107	3	0.6258835076090534
1107	4	0.7787459880953733
1107	5	0.564653164291748
1107	6	0.3815976812714439
1107	7	0.45898261815638364
1107	8	0.4504508990942
1107	9	0.4262348724442617
1107	10-11	0.4873672589199529
1107	12-13	0.42363395172109364
1107	14-15	0.6676584230193185
1107	16-17	0.7412281040300499
1107	18-19	0.79012425434302
1107	20-21	0.5357565382726222
1107	22-23	0.8198563789857971
1107	24-25	0.6060024345675856
1107	26-27	0.49727432017327544
1107	28-29	0.6343562588399578
1107	30-31	0.6826139909768187
1107	32-33	0.6539129743244416
1107	34-35	0.49649336558209
1107	36-37	0.45335355388174037
1107	38-39	0.4925104554070998
1107	40-41	0.42225947079057136
1107	42-43	0.5700542837811113
1107	44-45	0.47023876844382784
1107	46-47	0.5198110405512892
1107	48-49	0.590027510452785
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2214	3	-1.2640748314089905
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2214	94-95	-1.328754211909132
2214	96-97	-1.2335393836730972
2214	98-99	-1.4610251012868822
2214	100	-1.311470778234142
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2215	3	-3.790193569582268
2215	4	-2.134644752669182
2215	5	-1.7044469573830838
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2215	7	-1.3902286005070579
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2215	9	-1.8452679090768669
2215	10-11	-1.6499747916555023
2215	12-13	-1.619177885136537
2215	14-15	-4.114069715819937
2215	16-17	-3.7500736471750145
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2215	22-23	-3.454593401668369
2215	24-25	-5.839197434529275
2215	26-27	-3.8015732110041256
2215	28-29	-2.001308196939661
2215	30-31	-4.512641549506718
2215	32-33	-4.479632412847437
2215	34-35	-3.315853666117434
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2215	100	-3.304545844633914
2216	1	-0.6046618472487246
2216	2	-0.5824620463116759
2216	3	-0.839887900324463
2216	4	-0.7029919599141721
2216	5	-0.7936596789407986
2216	6	-0.7610154260907862
2216	7	-0.7705126654772556
2216	8	-0.6854811478691829
2216	9	-0.9615888592328119
2216	10-11	-0.920023460342513
2216	12-13	-1.2082826081103946
2216	14-15	-1.4843406386346985
2216	16-17	-1.1714399112818015
2216	18-19	-3.0503095593997287
2216	20-21	-1.4485108137987623
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2216	24-25	-1.0224766179025409
2216	26-27	-1.0370158373468499
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2216	30-31	-1.2344869941630208
2216	32-33	-1.2313590570300192
2216	34-35	-1.3211174607209344
2216	36-37	-1.421862092363682
2216	38-39	-1.424548251701907
2216	40-41	-1.452429371646197
2216	42-43	-1.459516678556362
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2216	50-51	-1.4283601130003092
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2216	54-55	-1.3172948675210208
2216	56-57	-1.3724253541454203
2216	58-59	-1.4407603543616503
2216	60-61	-1.393098674517006
2216	62-63	-1.4080379716872073
2216	64-65	-1.335326163621481
2216	66-67	-1.5119657172875947
2216	68-69	-1.592270100591655
2216	70-71	-1.7145537931442583
2216	72-73	-1.6295255856304145
2216	74-75	-1.5884291968006536
2216	76-77	-1.498773397794956
2216	78-79	-1.5317460936458218
2216	80-81	-1.5559393829751187
2216	82-83	-1.7634220433260346
2216	84-85	-1.5836174549104278
2216	86-87	-1.6507918374810728
2216	88-89	-1.3252674881307556
2216	90-91	-1.4603456537458257
2216	92-93	-1.3791854580169343
2216	94-95	-1.259507200939776
2216	96-97	-1.2595089416879013
2216	98-99	-1.2483223107629158
2216	100	-1.2666128212266372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	10910.0
1	1777.0
2	289.0
3	274.0
4	255.0
5	152.0
6	142.0
7	186.0
8	378.0
9	701.0
10	1061.0
11	1804.0
12	2882.0
13	4097.0
14	5522.0
15	7468.0
16	9443.0
17	11611.0
18	13364.0
19	14910.0
20	17079.0
21	19518.0
22	22356.0
23	25586.0
24	28326.0
25	32180.0
26	36401.0
27	41820.0
28	49531.0
29	58876.0
30	69926.0
31	83618.0
32	103358.0
33	131573.0
34	179200.0
35	240788.0
36	350018.0
37	520143.0
38	450755.0
39	142898.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77433046310769	20.635152352127715	12.320754752384401	24.269762432380197
2	15.429070065158665	20.811695267356548	40.373178726132814	23.386055941351977
3	18.170593839978018	27.786257033966145	29.578686335886072	24.464462790169772
4	12.098842396437224	17.465031114562063	40.051328761651625	30.384797727349085
5	12.916628572388147	38.925946588400805	35.07294510132456	13.084479737886486
6	28.938912994942584	38.607220167545165	19.112976872375807	13.340889965136448
7	26.087386761663645	32.44228859065415	24.432632058491848	17.03769258919036
8	25.641038088669294	34.75084609745833	21.045990433487948	18.56212538038443
9	26.903197638698984	14.99237910489123	21.434789899708946	36.66963335670084
10-11	25.114785217546565	25.966728348624336	28.235239846196023	20.68324658763308
12-13	25.689345762346573	24.191902789094925	27.830551011577075	22.288200436981427
14-15	23.117205310571784	25.225757569249346	25.431546054394722	26.22549106578415
16-17	22.26535025218785	27.609947216670133	26.65509599498874	23.469606536153275
18-19	21.932466069432905	28.046542100199012	27.955384516545443	22.065607313822635
20-21	23.509675838538747	27.07512882099862	26.76927524636648	22.64592009409615
22-23	23.766865619982177	26.89616585952145	26.49219187112261	22.844776649373767
24-25	23.224045042528047	27.138541481467094	27.03864576594559	22.59876771005927
26-27	23.010878491089688	27.466052094719164	26.463849957409913	23.059219456781236
28-29	22.50765912465173	27.85264230697484	26.695058069674648	22.944640498698785
30-31	23.060486205891408	27.450557129201574	26.591862408666234	22.89709425624078
32-33	23.341758300241917	27.797068783770285	25.84123776987049	23.01993514611731
34-35	23.402824026675223	27.422652648752948	26.72787022161994	22.44665310295188
36-37	23.256526816715645	27.248978789760326	26.55516477965993	22.9393296138641
38-39	23.375205336555837	27.144937023897022	26.960327903451898	22.519529736095247
40-41	23.02725684372302	26.69108947804319	27.32033207452438	22.961321603709408
42-43	22.89505230406475	26.918279544988444	26.811534649785223	23.375133501161585
44-45	22.79212135454241	27.75200969270204	26.69004910236777	22.76581985038778
46-47	22.904204675680802	27.32718838056616	26.433688961033504	23.33491798271954
48-49	23.389475189950527	26.700973884522107	26.82721229547183	23.082338630055535
50-51	23.115818719954486	26.951502651485228	26.382170603878958	23.550508024681328
52-53	22.640444569944766	27.40579632194418	26.356262540694225	23.59749656741683
54-55	22.701166921861173	27.35901141670119	26.335494050711922	23.604327610725715
56-57	23.430351535669185	26.833153616967714	26.29460212215957	23.44189272520353
58-59	22.757220099458518	26.855088896164325	27.082248165198187	23.30544283917897
60-61	22.804819131308125	27.50584948926568	26.37085528506421	23.318476094361987
62-63	23.121866758990254	26.88263364231109	26.11659438013788	23.878905218560774
64-65	22.280445827052738	27.08668840682699	26.51860049943477	24.114265266685507
66-67	23.008804769284268	26.585581214500138	26.402672992444998	24.0029410237706
68-69	22.362587584362117	26.780575371585584	26.568486254555506	24.28835078949679
70-71	22.44790968235001	26.451601697191286	26.751811362097232	24.348677258361477
72-73	22.911865415357617	26.572667201212987	26.98018872734832	23.53527865608108
74-75	22.905829787369154	26.982865256100386	26.660670138203418	23.45063481832704
76-77	22.55273305430142	26.749845890049624	26.663383718556855	24.034037337092105
78-79	22.36481967862333	26.793294503121484	26.703498081528497	24.13838773672668
80-81	22.184414752507884	26.87713308986734	27.16718195425815	23.77127020336663
82-83	22.72154283957751	26.051590584567048	27.790474831633826	23.436391744221616
84-85	22.94736932541279	26.512846603157385	26.720086631411956	23.81969744001787
86-87	21.92752195794768	26.983643738774195	27.16496155848643	23.9238727447917
88-89	22.66197152671261	26.01450058094373	26.973353609224464	24.350174283119195
90-91	22.418762364372274	25.953817637295717	27.24619273130815	24.38122726702386
92-93	22.638234359911515	26.14550162731416	27.140805172543008	24.075458840231313
94-95	21.666701105097953	26.117298549265428	27.62902671357807	24.586973632058548
96-97	22.004751420110363	25.98748432065173	27.26131044978082	24.746453809457083
98-99	21.601117781386513	26.614323724605654	27.094572284085366	24.68998620992247
100	22.15248381865778	25.89796072453648	26.65272065543498	25.29683480137076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9326.0
1	5536.0
2	1691.0
3	1355.0
4	863.0
5	596.0
6	523.5
7	480.0
8	434.5
9	429.5
10	412.5
11	376.0
12	366.0
13	357.0
14	390.5
15	446.0
16	484.5
17	539.0
18	641.5
19	789.0
20	968.5
21	1221.0
22	1616.5
23	2194.5
24	2947.0
25	3892.5
26	5370.0
27	7010.5
28	9586.0
29	14936.0
30	22003.5
31	28967.0
32	37667.5
33	45851.5
34	53631.5
35	61827.0
36	69515.0
37	82518.0
38	88288.0
39	83109.0
40	78574.5
41	74639.5
42	74665.5
43	79711.5
44	86383.0
45	97926.5
46	108660.0
47	116613.5
48	121728.5
49	118495.5
50	112908.0
51	112289.0
52	111565.5
53	108471.0
54	104169.0
55	94215.5
56	84565.5
57	71718.0
58	59624.0
59	54026.0
60	44443.5
61	33475.0
62	25985.0
63	20498.5
64	15013.0
65	9624.0
66	6542.0
67	4874.0
68	3673.0
69	2776.0
70	2187.0
71	1729.5
72	1311.5
73	994.5
74	755.5
75	585.5
76	441.0
77	282.0
78	178.5
79	108.0
80	79.5
81	52.5
82	34.5
83	31.0
84	27.0
85	22.5
86	23.0
87	20.5
88	17.0
89	15.0
90	15.0
91	11.0
92	7.5
93	6.5
94	2.5
95	1.5
96	1.5
97	0.5
98	1.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9877390017753516
2	0.4455842496817521
3	0.47805928589104585
4	0.4640140185144863
5	0.42890085007308737
6	0.47768771797103105
7	0.49485415587571496
8	0.49845836469985855
9	0.5124293184924151
10-11	0.5248025302289082
12-13	0.5227031714808245
14-15	0.5214769973447757
16-17	0.5249140006049126
18-19	0.5226474362928223
20-21	0.5215513109287786
22-23	0.4842087349672909
24-25	0.5204180287727335
26-27	0.49957306845990296
28-29	0.5056853607441465
30-31	0.508583590520262
32-33	0.5105529004963404
34-35	0.5223130251648089
36-37	0.5414487730455714
38-39	0.5309891360971546
40-41	0.5361353517893597
42-43	0.53338574918125
44-45	0.5441612188616795
46-47	0.5415788218175766
48-49	0.5136740710244647
50-51	0.5281280631130406
52-53	0.5207152831087453
54-55	0.529670069981102
56-57	0.5176312693726225
58-59	0.5239665024088749
60-61	0.5175012206006173
62-63	0.5112403011483678
64-65	0.5228517986488305
66-67	0.5201579312287231
68-69	0.5422290656776024
70-71	0.5264745858689748
72-73	0.5299115891291116
74-75	0.527384927273011
76-77	0.5137483846084677
78-79	0.545758960917743
80-81	0.5198420984967105
82-83	0.518300091628649
84-85	0.5279608575490339
86-87	0.8361578688053125
88-89	1.1028321649999366
90-91	1.0930041935155452
92-93	1.0922796360715163
94-95	1.0977231060997332
96-97	1.0908119427874579
98-99	1.086427441331283
100	1.0822658806271175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2691298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.51800067313878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62480977115554	32.643449050042435
2	11.818606862438251	9.813698553405478
3	3.920420416747557	4.883040525045361
4	1.7494161889892632	2.905290500482244
5	0.9378564843305792	1.9468963073872279
6	0.573140389292417	1.427738584106735
7	0.39352520476262765	1.1436865801362295
8	0.26742949320070886	0.8882510302979355
9	0.21332957893978646	0.7971315841820221
>10	1.1458405986055253	9.123262197113014
>50	0.14752135928130125	4.343602110621127
>100	0.17475695821564194	15.655354288021908
>500	0.023486112469589954	6.703899631351064
>1k	0.009412373317965413	6.539063673214314
>5k	4.482082532364482E-4	1.1856353845929026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	8094	0.3007470744599817	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7102	0.26388753679451327	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5863	0.21785027150467914	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5549	0.20618303881621433	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5249	0.19503600121577022	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	4923	0.18292288702328766	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4353	0.16174351558244385	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	3966	0.14736383707787098	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3451	0.1282280891971086	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3242	0.12046231966879922	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	3180	0.11815859856470744	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	2910	0.10812626472430774	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	2830	0.10515372136418932	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	2773	0.10303578422010495	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7156792001480323E-5
2	0.0	0.0	0.0	0.0	3.7156792001480323E-5
3	0.0	0.0	0.0	0.0	3.7156792001480323E-5
4	0.0	0.0	0.0	0.0	3.7156792001480323E-5
5	0.0	0.0	0.0	0.0	3.7156792001480323E-5
6	0.0	0.0	0.0	0.0	3.7156792001480323E-5
7	0.0	0.0	0.0	0.0	3.7156792001480323E-5
8	0.0	0.0	0.0	0.0	3.7156792001480323E-5
9	0.0	0.0	0.0	3.7156792001480323E-5	3.7156792001480323E-5
10-11	0.0	0.0	0.0	3.7156792001480323E-5	3.7156792001480323E-5
12-13	0.0	0.0	0.0	3.7156792001480323E-5	7.431358400296065E-5
14-15	0.0	0.0	0.0	3.7156792001480323E-5	1.3004877200518115E-4
16-17	0.0	0.0	0.0	3.7156792001480323E-5	1.486271680059213E-4
18-19	0.0	0.0	0.0	3.7156792001480323E-5	2.2294075200888195E-4
20-21	0.0	0.0	0.0	5.573518800222049E-5	2.9725433601184264E-4
22-23	0.0	0.0	0.0	1.857839600074016E-4	3.529895240140631E-4
24-25	0.0	1.8578396000740162E-5	0.0	5.573518800222048E-4	3.715679200148033E-4
26-27	0.0	3.7156792001480323E-5	0.0	0.001486271680059213	3.715679200148033E-4
28-29	0.0	3.7156792001480323E-5	0.0	0.003641365616145072	3.715679200148033E-4
30-31	0.0	3.7156792001480323E-5	0.0	0.012596152488501832	3.715679200148033E-4
32-33	0.0	3.7156792001480323E-5	0.0	0.033534004781335995	3.715679200148033E-4
34-35	0.0	3.7156792001480323E-5	0.0	0.06545168911060759	4.087247120162836E-4
36-37	0.0	3.7156792001480323E-5	0.0	0.11899462638474073	4.087247120162836E-4
38-39	0.0	3.7156792001480323E-5	0.0	0.19888172918792346	4.458815040177639E-4
40-41	0.0	3.7156792001480323E-5	0.0	0.2934457648316909	4.458815040177639E-4
42-43	0.0	3.7156792001480323E-5	0.0	0.39018347280754495	4.458815040177639E-4
44-45	0.0	3.7156792001480323E-5	0.0	0.5072459460082086	4.644599000185041E-4
46-47	0.0	3.7156792001480323E-5	0.0	0.6479772957138155	4.8303829601924425E-4
48-49	0.0	3.7156792001480323E-5	0.0	0.8089405186642282	5.016166920199844E-4
50-51	0.0	3.7156792001480323E-5	0.0	0.9907858587194729	5.201950880207246E-4
52-53	0.0	3.7156792001480323E-5	0.0	1.1736344321587575	5.201950880207246E-4
54-55	0.0	5.573518800222049E-5	0.0	1.365382057282397	5.201950880207246E-4
56-57	0.0	7.431358400296065E-5	0.0	1.598540927091686	5.201950880207246E-4
58-59	0.0	7.431358400296065E-5	0.0	1.8738355990306537	5.387734840214648E-4
60-61	0.0	7.431358400296065E-5	0.0	2.1712942973985045	5.573518800222049E-4
62-63	0.0	7.431358400296065E-5	0.0	2.488000214023122	5.573518800222049E-4
64-65	0.0	7.431358400296065E-5	0.0	2.8126391057400557	5.759302760229451E-4
66-67	0.0	7.431358400296065E-5	0.0	3.176478413018551	5.945086720236852E-4
68-69	0.0	7.431358400296065E-5	0.0	3.5828436687427407	5.945086720236852E-4
70-71	0.0	7.431358400296065E-5	0.0	4.033852810056708	5.945086720236852E-4
72-73	0.0	7.431358400296065E-5	0.0	4.519993698208077	6.87400652027386E-4
74-75	0.0	7.431358400296065E-5	0.0	4.994615980838986	7.802926320310869E-4
76-77	0.0	7.431358400296065E-5	0.0	5.517114789963802	8.174494240325672E-4
78-79	0.0	7.431358400296065E-5	0.0	6.081879449990302	8.174494240325672E-4
80-81	0.0	7.431358400296065E-5	0.0	6.704032775263089	8.360278200333074E-4
82-83	0.0	7.431358400296065E-5	0.0	7.374397038157795	8.546062160340475E-4
84-85	0.0	7.431358400296065E-5	0.0	8.038816214332266	8.546062160340475E-4
86-87	0.0	7.431358400296065E-5	0.0	8.71163282549907	9.28919800037008E-4
88	0.0	7.431358400296065E-5	0.0	9.236249571767972	0.0010032333840399687
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6270	0.0	31.079044	1
GTACTAG	1775	0.0	29.771778	1
CAACGCA	6660	0.0	28.639465	5
CGTTAAC	575	0.0	27.899485	1
TAGTACT	2275	0.0	27.87929	4
GGTATCA	4265	0.0	27.546436	1
GGGCACC	2145	0.0	27.296434	94
ATCAACG	6990	0.0	27.222723	3
GTACATG	11785	0.0	26.904491	1
TACATGG	11810	0.0	26.577417	2
CTAACGC	1045	0.0	26.527079	3
CTGTGCG	2445	0.0	26.116888	9
ACATGGG	11940	0.0	25.971277	3
TCTAACG	1080	0.0	25.669327	2
TATCAAC	7640	0.0	25.646547	2
TATTCTC	1705	0.0	25.349916	5
AACGCAG	7880	0.0	25.318316	6
ATTTAGG	3205	0.0	25.026842	1
ACGCAGA	7620	0.0	24.582758	7
AGGGCTA	3125	0.0	24.504772	5
>>END_MODULE