FastQCFastQC Report
Mon 16 Jan 2017
ERR1146461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691298
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA76130.28287465750726976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70710.26273567624246735No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC65230.24237375422565618No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC64780.24070169858558957No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57810.21480341456055776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.21186802799244084No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC54110.20105540152001006No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC46540.17292770997488943No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT39270.14591472218981325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38950.14472570484576586No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38410.14271923807768594No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA34670.1288225978691323No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC30740.11421997861255052No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA28490.10585970041221746No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG28040.10418764477215083No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG27110.10073206311601317No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC27080.10062059274000873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.044.861721
GTATCAA101150.040.2603951
ATCAACG127050.031.4567433
CAACGCA127550.031.320045
TATCAAC131900.030.8008482
AACGCAG141100.028.97696
TCAACGC147150.027.3511074
GGGCACC18500.027.20565494
GCTATTG33100.026.976488
GTACTAG17650.026.9093171
ACGCAGA148750.026.8231897
AGGGCTA32700.026.7330845
ATTTAGG33450.026.5700071
CGCAGAG150800.026.396228
CTATTGA33400.025.894279
GCAGAGT162450.024.1601189
GGGCTAT37000.024.1330136
GTACATG110000.024.0253451
GGCTATT38050.023.3435467
TACATGG112050.023.2063562
Produced by FastQC (version 0.11.5)