FastQCFastQC Report
Mon 16 Jan 2017
ERR1146460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912236
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120350.30762459115452134No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC93610.23927493126692767No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT77050.19694619649734832No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA70160.17933478450686513No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60470.15456634006741923No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA54060.13818184792532964No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC52840.13506342664399593No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG43330.11075507714769764No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC42840.10950259646912916No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT39690.10145093496404614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG27500.032.9314081
GTATCAA87300.030.2022531
CAACGCA83500.029.4858065
GGGCACC34850.028.3132294
ATCAACG87550.028.1236213
TAGGCAT37900.028.0180175
GCATCGT32500.026.5821388
GTACATG151050.026.1052171
AACGCAG99300.026.049326
CTAGGCA41450.025.9584714
TAGTACT41150.025.9193554
TATCAAC102600.025.465132
GGTATCA59350.025.4314441
TACATGG152850.025.2711412
ACGCAGA99350.024.716317
ACATGGG151750.024.647933
CTGTGCG43750.023.6129539
TCAACGC107650.023.4384234
CGCAGAG103200.023.2485928
CTAGTAC46350.022.9115683
Produced by FastQC (version 0.11.5)