##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146460_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3912236 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.434710993917545 31.0 30.0 34.0 27.0 34.0 2 30.544310465933037 31.0 31.0 34.0 26.0 34.0 3 30.795722701800198 31.0 31.0 34.0 27.0 34.0 4 34.266889829754646 37.0 35.0 37.0 30.0 37.0 5 34.52325933302592 37.0 35.0 37.0 30.0 37.0 6 34.66035791296844 37.0 35.0 37.0 31.0 37.0 7 34.53847799570374 37.0 35.0 37.0 30.0 37.0 8 34.487985131776306 37.0 35.0 37.0 30.0 37.0 9 35.89863546064195 39.0 35.0 39.0 30.0 39.0 10-11 35.825532125362585 39.0 35.0 39.0 30.0 39.0 12-13 35.830964952012096 39.0 35.0 39.0 30.0 39.0 14-15 36.87427138853587 40.0 36.0 41.0 30.0 41.0 16-17 36.70249481370756 40.0 36.0 41.0 29.0 41.0 18-19 36.63657126001601 40.0 36.0 41.0 28.5 41.0 20-21 36.964438750627515 40.0 36.0 41.0 30.0 41.0 22-23 36.686540893749765 40.0 36.0 41.0 29.0 41.0 24-25 36.803207168483695 40.0 36.0 41.0 30.0 41.0 26-27 36.87572541636036 40.0 36.0 41.0 30.0 41.0 28-29 36.7001696727907 40.0 36.0 41.0 29.5 41.0 30-31 36.63469432825627 40.0 36.0 41.0 29.5 41.0 32-33 36.73298760606467 40.0 36.0 41.0 30.0 41.0 34-35 36.78532698436393 40.0 36.0 41.0 30.0 41.0 36-37 36.72379069156359 40.0 35.0 41.0 30.0 41.0 38-39 36.56723661353763 39.5 35.0 41.0 29.0 41.0 40-41 36.534688219217855 39.0 35.0 41.0 29.0 41.0 42-43 36.37552898138047 39.0 35.0 41.0 29.0 41.0 44-45 36.21488772660954 39.0 35.0 41.0 28.0 41.0 46-47 36.089942682394415 39.0 35.0 41.0 28.0 41.0 48-49 35.96982812386574 39.0 34.5 41.0 28.0 41.0 50-51 35.76723285609559 38.5 34.0 40.0 27.5 41.0 52-53 35.59781554589242 38.0 34.0 40.0 27.0 41.0 54-55 35.40304547067201 38.0 34.0 40.0 27.0 41.0 56-57 35.27774998747519 37.5 34.0 40.0 27.0 41.0 58-59 35.033082487866274 37.0 33.0 40.0 26.5 41.0 60-61 34.77059947303792 37.0 33.0 40.0 26.0 41.0 62-63 34.50450279584361 36.0 33.0 39.5 26.0 41.0 64-65 34.21903177620164 36.0 33.0 39.0 26.0 41.0 66-67 33.91351237502032 35.0 32.0 39.0 26.0 41.0 68-69 33.61974098699567 35.0 32.0 38.5 26.0 40.0 70-71 33.511839520928696 35.0 32.0 38.0 26.0 40.0 72-73 33.276830692217956 35.0 32.0 37.0 26.0 40.0 74-75 33.00610942182425 35.0 32.0 37.0 25.0 39.0 76-77 32.75989804807276 35.0 32.0 36.0 25.0 39.0 78-79 32.53541248534086 35.0 32.0 36.0 25.0 39.0 80-81 32.28806799998773 35.0 31.5 35.5 24.5 37.5 82-83 32.07047874923701 35.0 31.0 35.0 24.0 37.0 84-85 31.88692936724676 35.0 31.0 35.0 24.0 36.5 86-87 31.71988665816684 34.5 31.0 35.0 24.0 36.0 88-89 31.711333237565423 35.0 32.0 35.0 24.0 36.0 90-91 31.638308374034693 35.0 32.0 35.0 24.0 36.0 92-93 31.50677681510011 35.0 31.0 35.0 24.0 35.5 94-95 31.478725209828855 35.0 31.0 35.0 24.0 35.0 96-97 31.395333768208257 35.0 31.0 35.0 23.5 35.0 98-99 31.31318049320133 35.0 31.0 35.0 23.0 35.0 100 30.83421628961034 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8298900224800541 1101 2 0.9415342153795336 1101 3 0.9136768908039983 1101 4 1.0688924055203302 1101 5 0.9393502969032426 1101 6 0.6693368291420185 1101 7 0.7586093614272045 1101 8 0.7643967384479069 1101 9 1.011859093546775 1101 10-11 1.0137313052696442 1101 12-13 0.9690340468981233 1101 14-15 1.1295523685487225 1101 16-17 1.225459634030166 1101 18-19 1.4577358648423342 1101 20-21 1.0591781361450074 1101 22-23 1.3587837020066473 1101 24-25 1.2091239316819866 1101 26-27 1.091788222288102 1101 28-29 1.288020055623143 1101 30-31 1.3127473741876656 1101 32-33 1.1857369325242644 1101 34-35 1.0913870241751624 1101 36-37 1.0434463991442158 1101 38-39 1.1369248045355747 1101 40-41 0.9940497448706367 1101 42-43 1.015662670796921 1101 44-45 1.0316625289627126 1101 46-47 1.1116842901134234 1101 48-49 1.092429092414399 1101 50-51 1.0646439223600481 1101 52-53 1.0061017003961652 1101 54-55 1.033153767142764 1101 56-57 0.9111159508140219 1101 58-59 0.8758188069245207 1101 60-61 0.8971869222083981 1101 62-63 0.8096513241088914 1101 64-65 0.8712929704402512 1101 66-67 0.8286350375776195 1101 68-69 0.7989852980874019 1101 70-71 0.8314738680206162 1101 72-73 0.7815285606506208 1101 74-75 0.7416873075994488 1101 76-77 0.7592854439088157 1101 78-79 0.7691049372637977 1101 80-81 0.7794228953738909 1101 82-83 0.7001443646225525 1101 84-85 0.7661915436436395 1101 86-87 0.7788042985039247 1101 88-89 0.8026938109768693 1101 90-91 0.7908670115443499 1101 92-93 0.9014367504770462 1101 94-95 0.8445274777917255 1101 96-97 0.8101657107132745 1101 98-99 0.8559652193308658 1101 100 0.8786708328409318 1102 1 0.7316204531412289 1102 2 0.964396946841422 1102 3 0.915403970315424 1102 4 1.0293180056141509 1102 5 0.8606861181813912 1102 6 0.6367104602985236 1102 7 0.6873485741678849 1102 8 0.7448100397348156 1102 9 0.8716536555478598 1102 10-11 0.9291180787561757 1102 12-13 0.8509572172919135 1102 14-15 1.0149166543938222 1102 16-17 1.1735774306413447 1102 18-19 1.222718917952875 1102 20-21 0.9483977581836456 1102 22-23 1.2195712350075425 1102 24-25 1.0476799634734704 1102 26-27 0.9897362210038878 1102 28-29 1.1277772606023788 1102 30-31 1.225644572413941 1102 32-33 1.0541985693277027 1102 34-35 0.9541947967812163 1102 36-37 0.8293701493946344 1102 38-39 0.9425727933027304 1102 40-41 0.9150663480270467 1102 42-43 0.9812731924870874 1102 44-45 1.0041458045855194 1102 46-47 0.9053612790491812 1102 48-49 0.8606665894159065 1102 50-51 0.8899930613576856 1102 52-53 0.8727724810285977 1102 54-55 0.9414826727949759 1102 56-57 0.8134244329699953 1102 58-59 0.7900192559320018 1102 60-61 0.7655958460959837 1102 62-63 0.7317072786434267 1102 64-65 0.6655861662291187 1102 66-67 0.7682124246946103 1102 68-69 0.727545947827295 1102 70-71 0.7016311691263084 1102 72-73 0.6490999736953711 1102 74-75 0.6353163264716031 1102 76-77 0.6348485629889709 1102 78-79 0.6110113326928897 1102 80-81 0.6154639489672178 1102 82-83 0.6553040739850005 1102 84-85 0.6589895443436298 1102 86-87 0.7084418290052845 1102 88-89 0.6482920029980974 1102 90-91 0.7254730073189961 1102 92-93 0.7714325063667218 1102 94-95 0.7360971263440383 1102 96-97 0.7816314130640301 1102 98-99 0.7860912141179206 1102 100 0.7612982250807221 1103 1 0.47098131980039426 1103 2 0.6881949630637507 1103 3 0.6727613248380031 1103 4 0.6124047892663853 1103 5 0.6096139777840364 1103 6 0.3630074060193067 1103 7 0.375532256236923 1103 8 0.4915347507911747 1103 9 0.6390607447984564 1103 10-11 0.6936683120901108 1103 12-13 0.5763623624645078 1103 14-15 0.7222522842250001 1103 16-17 0.931560566407164 1103 18-19 0.9424201844355196 1103 20-21 0.6372967686547639 1103 22-23 0.8843862552257917 1103 24-25 0.7701228414326309 1103 26-27 0.6675332192992158 1103 28-29 0.8392501527238849 1103 30-31 0.8855440698223163 1103 32-33 0.7527252760139547 1103 34-35 0.6674154973572897 1103 36-37 0.6988029186202027 1103 38-39 0.7134347246946646 1103 40-41 0.6626243852057669 1103 42-43 0.684666911809316 1103 44-45 0.7412564547188651 1103 46-47 0.7779643848016207 1103 48-49 0.7085000070520593 1103 50-51 0.7185670221976466 1103 52-53 0.7258023871574792 1103 54-55 0.7082368413266593 1103 56-57 0.6258603700410177 1103 58-59 0.7070976690881707 1103 60-61 0.6505403334129269 1103 62-63 0.5589578294961157 1103 64-65 0.6365182144886177 1103 66-67 0.67811474093741 1103 68-69 0.6820246561875365 1103 70-71 0.5768297002906877 1103 72-73 0.5771415550431342 1103 74-75 0.5896143672112331 1103 76-77 0.5152114610444514 1103 78-79 0.5060555071061046 1103 80-81 0.46955072134410614 1103 82-83 0.39723611096915334 1103 84-85 0.41974169327876965 1103 86-87 0.5043014463308033 1103 88-89 0.4840547782835003 1103 90-91 0.4885778996880248 1103 92-93 0.5313118654975071 1103 94-95 0.5416496008480145 1103 96-97 0.4551988486300722 1103 98-99 0.5067584886039533 1103 100 0.5848701314492715 1104 1 0.31659281435858944 1104 2 0.5533678692376469 1104 3 0.6424092433861652 1104 4 0.6944510691272896 1104 5 0.5842062113288264 1104 6 0.2718210289368699 1104 7 0.34570982809897544 1104 8 0.30686682255111464 1104 9 0.40387284377871424 1104 10-11 0.4750749859550325 1104 12-13 0.41662062215480944 1104 14-15 0.6627911584553843 1104 16-17 0.711062551812887 1104 18-19 0.6500614790675812 1104 20-21 0.5552985344378314 1104 22-23 0.8252049949680043 1104 24-25 0.6314508282781759 1104 26-27 0.5635621688617221 1104 28-29 0.7430857106565085 1104 30-31 0.7720626923692961 1104 32-33 0.5536196681910823 1104 34-35 0.4969446707393388 1104 36-37 0.4900373675771803 1104 38-39 0.5493349369960754 1104 40-41 0.4836045087651186 1104 42-43 0.4030466774614112 1104 44-45 0.4308459210852078 1104 46-47 0.3760930732738714 1104 48-49 0.4587670560203989 1104 50-51 0.5791104821129665 1104 52-53 0.4920906255226285 1104 54-55 0.4363670602064076 1104 56-57 0.3980369467155924 1104 58-59 0.3664459364124397 1104 60-61 0.4735676648121441 1104 62-63 0.3597157936117199 1104 64-65 0.4170228047930493 1104 66-67 0.5039770859268202 1104 68-69 0.490184083088252 1104 70-71 0.4124470597108427 1104 72-73 0.40442824863226434 1104 74-75 0.36432024028918164 1104 76-77 0.37211843258251065 1104 78-79 0.37940402735127776 1104 80-81 0.4066272052586726 1104 82-83 0.37759202936914704 1104 84-85 0.39504671316985096 1104 86-87 0.3597886450447625 1104 88-89 0.3097372982056825 1104 90-91 0.3068013370264069 1104 92-93 0.32760279424384464 1104 94-95 0.2566580982382014 1104 96-97 0.3131453810125855 1104 98-99 0.334527809940365 1104 100 0.3904351664380705 1105 1 0.44400410242591803 1105 2 0.5973190641990271 1105 3 0.6638067251038073 1105 4 0.7539138803352117 1105 5 0.6118790797147966 1105 6 0.3153814408152442 1105 7 0.3774687686816165 1105 8 0.4664333406947776 1105 9 0.46223164832434094 1105 10-11 0.4875201480555873 1105 12-13 0.43818017015765065 1105 14-15 0.6379736794788968 1105 16-17 0.7535352755064508 1105 18-19 0.7827174082143031 1105 20-21 0.6609241244949118 1105 22-23 0.8632651765378867 1105 24-25 0.6899918892814938 1105 26-27 0.5038241518366462 1105 28-29 0.7533672978874151 1105 30-31 0.7330116580086923 1105 32-33 0.6042227793303354 1105 34-35 0.5294541178902605 1105 36-37 0.42389462059121286 1105 38-39 0.5720267550347842 1105 40-41 0.5085995694860586 1105 42-43 0.5674617336057182 1105 44-45 0.561810278002838 1105 46-47 0.49177858885272485 1105 48-49 0.605427967149545 1105 50-51 0.6791326110008811 1105 52-53 0.6763657419753244 1105 54-55 0.5500081487446451 1105 56-57 0.44336659415703394 1105 58-59 0.4931045669698264 1105 60-61 0.5205903758022359 1105 62-63 0.530476557877428 1105 64-65 0.4869083480244143 1105 66-67 0.6002506683852715 1105 68-69 0.5669504026914041 1105 70-71 0.4395474047821111 1105 72-73 0.3935368981165013 1105 74-75 0.4214250028210671 1105 76-77 0.42073980946107525 1105 78-79 0.3360593119037887 1105 80-81 0.45378628595078396 1105 82-83 0.5128337775753806 1105 84-85 0.43880145545849913 1105 86-87 0.5378725053277371 1105 88-89 0.4129967950648634 1105 90-91 0.4261007357464379 1105 92-93 0.5064845248307108 1105 94-95 0.43107248070758075 1105 96-97 0.442430239653703 1105 98-99 0.5198124206782815 1105 100 0.5796663823146346 1106 1 0.47920377242409273 1106 2 0.5374784556209597 1106 3 0.5244095182435835 1106 4 0.6586871997595267 1106 5 0.5928435275960098 1106 6 0.2965355520280184 1106 7 0.31486235366383397 1106 8 0.35155145489808604 1106 9 0.5294971293055966 1106 10-11 0.48690703167505944 1106 12-13 0.4189760409063368 1106 14-15 0.633674231611181 1106 16-17 0.7098980191954354 1106 18-19 0.6973763875837875 1106 20-21 0.5606398066216371 1106 22-23 0.835072239616089 1106 24-25 0.7359023081355076 1106 26-27 0.5812918387829455 1106 28-29 0.7771105407446512 1106 30-31 0.7749646049708829 1106 32-33 0.6117861740037043 1106 34-35 0.632659717777436 1106 36-37 0.530327111803615 1106 38-39 0.693800327605949 1106 40-41 0.637954713034155 1106 42-43 0.6370011924629964 1106 44-45 0.7088098327747758 1106 46-47 0.62193282905681 1106 48-49 0.5742407477927927 1106 50-51 0.6834428831174435 1106 52-53 0.6318116999027907 1106 54-55 0.6381315355098423 1106 56-57 0.4923589156317121 1106 58-59 0.6111871409750691 1106 60-61 0.6248389974958073 1106 62-63 0.5480010308011742 1106 64-65 0.5892108260070472 1106 66-67 0.6025547573535164 1106 68-69 0.6116616915788455 1106 70-71 0.5153706212382971 1106 72-73 0.48123976984313543 1106 74-75 0.5492620946709721 1106 76-77 0.5715675074374289 1106 78-79 0.5952099491231095 1106 80-81 0.5669840592031861 1106 82-83 0.5929612636101282 1106 84-85 0.5963485378482893 1106 86-87 0.5562653360425607 1106 88-89 0.5279240161894805 1106 90-91 0.47014951031776775 1106 92-93 0.5767867136793896 1106 94-95 0.598076104834778 1106 96-97 0.5780501491641097 1106 98-99 0.5440171842024633 1106 100 0.5396636135561259 1107 1 0.5092955977595359 1107 2 0.6636109271710637 1107 3 0.5884509378534482 1107 4 0.7504718705755167 1107 5 0.6686646461955519 1107 6 0.41192028545219017 1107 7 0.4300019484293003 1107 8 0.45638026448327196 1107 9 0.5924574835942593 1107 10-11 0.6100688753632966 1107 12-13 0.4699671836898247 1107 14-15 0.6971310012744496 1107 16-17 0.787086198218006 1107 18-19 0.9219609945639817 1107 20-21 0.6514210969026379 1107 22-23 0.8859895338991066 1107 24-25 0.603714259546642 1107 26-27 0.6255875120664456 1107 28-29 0.8268681258488115 1107 30-31 0.8203212030754514 1107 32-33 0.6923761794051941 1107 34-35 0.5447821121721645 1107 36-37 0.5696849098983563 1107 38-39 0.6053791203114827 1107 40-41 0.5308383262628951 1107 42-43 0.6628621700713708 1107 44-45 0.5691346971237152 1107 46-47 0.4936288279642369 1107 48-49 0.5178857102892707 1107 50-51 0.6149225999074872 1107 52-53 0.7029398901580137 1107 54-55 0.6185657109846261 1107 56-57 0.4742412889729053 1107 58-59 0.6302346832969761 1107 60-61 0.5972426760334812 1107 62-63 0.6301464582554814 1107 64-65 0.6820688895727613 1107 66-67 0.6416865982182429 1107 68-69 0.65974723154374 1107 70-71 0.5977180291367503 1107 72-73 0.5946867953406212 1107 74-75 0.5713278347979056 1107 76-77 0.47503543301962026 1107 78-79 0.4549018263251341 1107 80-81 0.5784142725620569 1107 82-83 0.5823951736139392 1107 84-85 0.5306713259993892 1107 86-87 0.5843920240082845 1107 88-89 0.5557296070388631 1107 90-91 0.6305398217014044 1107 92-93 0.6234407436208329 1107 94-95 0.6486080442153259 1107 96-97 0.5276812519274614 1107 98-99 0.6261144662707459 1107 100 0.6898073975456178 1108 1 0.33292069076961894 1108 2 0.4297699002559021 1108 3 0.41959209482415005 1108 4 0.526490046742552 1108 5 0.45925905171101533 1108 6 0.1332980420097698 1108 7 0.2550090898189268 1108 8 0.23604046872540607 1108 9 0.2441010890644435 1108 10-11 0.3654262077078201 1108 12-13 0.23606855798669102 1108 14-15 0.36378426495669913 1108 16-17 0.5866424967193851 1108 18-19 0.6080740477340925 1108 20-21 0.2875596892086918 1108 22-23 0.5612350154717376 1108 24-25 0.4231095373168614 1108 26-27 0.37825385483959906 1108 28-29 0.5578783725737466 1108 30-31 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-0.9452349995839384 2216 6 -0.8709901969034277 2216 7 -0.9591972454624909 2216 8 -0.8902272455105091 2216 9 -1.027806011405012 2216 10-11 -1.1339025886341147 2216 12-13 -1.3082806387524784 2216 14-15 -1.7118827832074004 2216 16-17 -1.337259418809424 2216 18-19 -3.369030671483415 2216 20-21 -1.6130904580227678 2216 22-23 -1.1282552448968843 2216 24-25 -1.3375345151901143 2216 26-27 -1.1969866780409433 2216 28-29 -1.1616407184581234 2216 30-31 -1.3782771360711195 2216 32-33 -1.2831780740521808 2216 34-35 -1.4893279043769994 2216 36-37 -1.5133786984168793 2216 38-39 -1.551874546238409 2216 40-41 -1.6083188705392502 2216 42-43 -1.5174685654876257 2216 44-45 -1.4278869253076607 2216 46-47 -1.5702200428669713 2216 48-49 -1.4927900285351328 2216 50-51 -1.3831648246157044 2216 52-53 -1.4129635500548403 2216 54-55 -1.354953440671217 2216 56-57 -1.3519265397524975 2216 58-59 -1.4153685040191775 2216 60-61 -1.4281151863525068 2216 62-63 -1.464615416034576 2216 64-65 -1.5486224508386854 2216 66-67 -1.4790053948805735 2216 68-69 -1.5134136252224408 2216 70-71 -1.6722929574395167 2216 72-73 -1.6212893448078667 2216 74-75 -1.6426936590619157 2216 76-77 -1.4683909977123548 2216 78-79 -1.5715311517260915 2216 80-81 -1.568480857289309 2216 82-83 -1.673433968365618 2216 84-85 -1.5271345347006147 2216 86-87 -1.5750009629839745 2216 88-89 -1.4821604916152253 2216 90-91 -1.5037364482768254 2216 92-93 -1.4668420145022765 2216 94-95 -1.4011418949392684 2216 96-97 -1.3558652418242367 2216 98-99 -1.2709082521642507 2216 100 -1.3488239780008584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16072.0 1 2689.0 2 438.0 3 435.0 4 418.0 5 213.0 6 193.0 7 259.0 8 441.0 9 785.0 10 1379.0 11 2544.0 12 4171.0 13 6160.0 14 8108.0 15 10360.0 16 12503.0 17 14675.0 18 17337.0 19 19560.0 20 22404.0 21 25778.0 22 29944.0 23 34151.0 24 38432.0 25 43328.0 26 49686.0 27 57170.0 28 67708.0 29 82090.0 30 97316.0 31 117571.0 32 144627.0 33 184431.0 34 252501.0 35 343223.0 36 510497.0 37 788171.0 38 696016.0 39 208259.0 40 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29172903094878 19.936758293212346 12.788101036979125 23.98341163885975 2 15.572390702069258 19.54303746440612 40.60680988951454 24.277761944010084 3 18.86624237850689 25.811773457040953 28.839539861905145 26.482444302547016 4 12.54622989299807 16.41935819387214 38.842586588355246 32.19182532477455 5 13.583574252410624 37.965158236403035 34.721177833065866 13.730089678120475 6 31.047555199375314 37.32266333775134 17.8891697233096 13.740611739563748 7 27.833197602575204 31.505172754854836 22.80903581493689 17.852593827633072 8 26.232997970662336 35.15322026008098 19.78112235552177 18.83265941373491 9 26.122925124621 15.570918341127499 20.773369649005602 37.5327868852459 10-11 25.673787552459665 25.695219851962943 27.5825471182937 21.04844547728369 12-13 25.99385074626099 23.333756920608735 27.56586921329564 23.106523119834637 14-15 23.34523416569777 24.712842684290194 24.818008747092517 27.123914402919517 16-17 22.77171936776669 27.51916140812097 25.917582495242574 23.79153672886977 18-19 22.422425586421667 27.733990787064677 27.367423840895377 22.47615978561828 20-21 23.821845803924045 26.851745849293142 26.486390363609335 22.840017983173475 22-23 24.069750640112975 26.469536915910957 26.369186724182107 23.091525719793957 24-25 23.596915913823512 26.6513479097079 26.7360447721594 23.015691404309184 26-27 23.463611295449752 26.872822780813266 26.08960836196042 23.573957561776567 28-29 22.881142439739662 27.214403875140142 26.574683560240043 23.32977012488016 30-31 23.445689178946285 26.8676627397577 26.450378345045543 23.236269736250463 32-33 23.686649147228163 26.992838271087084 25.830555639419895 23.489956942264865 34-35 23.7501487252798 26.912028900630443 26.395582775672427 22.942239598417334 36-37 23.5230670414768 26.640426187600575 26.17654852710875 23.659958243813872 38-39 23.598377663015942 26.7445307549742 26.590523620402546 23.066567961607312 40-41 23.304416571004943 26.15734407674561 27.05896518795681 23.479274164292633 42-43 23.279548648122482 26.290411599655872 26.605126860795263 23.824912891426383 44-45 23.462685163087713 26.845513553790873 26.429933965036927 23.26186731808449 46-47 23.414153821479367 26.704804159808898 26.14885728242217 23.732184736289565 48-49 23.83926500884243 26.447232252349362 26.285646743525888 23.427855995282325 50-51 23.518274898699794 26.487507443158936 26.315694792063134 23.678522866078136 52-53 23.287233848455546 26.744104173289106 26.192097576243643 23.776564402011697 54-55 23.131669396832873 26.697890104942207 26.303283857958714 23.867156640266206 56-57 23.899837892419 26.35364070088755 26.033472767866495 23.713048638826955 58-59 23.209728413704028 26.532492933637226 26.615125498448727 23.64265315421002 60-61 23.13852647548644 26.956190812936953 26.287388082118813 23.617894629457798 62-63 23.40992707635688 26.57720616150247 25.95193162668121 24.060935135459445 64-65 22.851004374802127 26.63084985876461 26.295801279547387 24.222344486885873 66-67 23.376373911586548 26.370211160689966 26.200839585511186 24.052575342212297 68-69 22.96282216965177 26.458958549878016 26.353552052458046 24.22466722801217 70-71 22.925444921983818 26.147936238385462 26.51371900605565 24.41289983357507 72-73 23.280774438870793 26.238940543446667 26.76292755286579 23.71735746481675 74-75 23.367857924834166 26.55381220774068 26.436111816399727 23.64221805102543 76-77 23.180845655417446 26.35517229461791 26.231614196551806 24.232367853412843 78-79 23.07951025217376 26.343082057487845 26.291610418425226 24.285797271913168 80-81 22.802870407286026 26.621574412049227 26.687410148802382 23.888145031862365 82-83 23.38887968805268 25.915684850359927 27.33113464041169 23.3643008211757 84-85 23.404556874322825 26.257100681130847 26.506125654557906 23.832216789988426 86-87 22.53836846643042 26.71930648358092 26.858113398895295 23.88421165109337 88-89 23.2562254306931 25.786062727857377 26.69653584404681 24.26117599740271 90-91 23.024471098531453 25.832523807302266 26.811825227152987 24.3311798670133 92-93 23.144126078608775 26.048536764807555 26.78716836305793 24.02016879352574 94-95 22.421181229248567 25.90081133729462 27.287796313099506 24.390211120357304 96-97 22.611989540690328 25.82296504422617 26.999030004220632 24.566015410862867 98-99 22.21501877605452 26.29217505020301 26.86194366408308 24.630862509659394 100 22.725762050618332 25.779262464633018 26.369840710306537 25.125134774442117 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13416.0 1 7914.0 2 2251.0 3 1634.0 4 892.5 5 532.5 6 437.0 7 424.5 8 418.5 9 407.0 10 398.0 11 363.0 12 376.0 13 387.5 14 370.0 15 423.0 16 487.0 17 587.0 18 744.0 19 900.0 20 1200.5 21 1591.0 22 2164.0 23 3119.5 24 4199.0 25 5437.0 26 7483.5 27 10065.0 28 13636.5 29 19693.5 30 27956.0 31 36342.0 32 46723.5 33 56406.0 34 64648.5 35 74672.5 36 84761.5 37 100487.5 38 109218.0 39 106484.5 40 103931.0 41 102116.5 42 104939.5 43 113121.0 44 124096.0 45 142460.0 46 159737.0 47 172673.5 48 183059.5 49 182737.5 50 175849.0 51 176884.5 52 177628.5 53 171492.0 54 163928.0 55 146709.5 56 130510.5 57 111886.0 58 93203.0 59 85325.5 60 71681.0 61 54784.0 62 43499.5 63 34718.0 64 25361.5 65 15905.5 66 10719.0 67 7798.0 68 5765.5 69 4332.5 70 3281.0 71 2546.0 72 1881.5 73 1366.5 74 1029.5 75 746.0 76 501.0 77 317.5 78 199.0 79 129.0 80 84.0 81 74.5 82 63.0 83 43.0 84 31.0 85 20.0 86 14.0 87 16.5 88 12.5 89 12.5 90 12.0 91 8.5 92 9.0 93 7.0 94 8.5 95 7.0 96 3.0 97 3.0 98 2.0 99 3.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0009365488176072 2 0.450023976058704 3 0.48488894841722224 4 0.46983361944422575 5 0.4366556618772487 6 0.48759839641575814 7 0.5042129360294215 8 0.5069479448581323 9 0.5223611254535769 10-11 0.5345536414469884 12-13 0.5334417453343817 14-15 0.5309367839772448 16-17 0.5355249529936333 18-19 0.533186137032633 20-21 0.5321892646558132 22-23 0.4950749392419067 24-25 0.5293775733365779 26-27 0.5097596361773676 28-29 0.5149101434576033 30-31 0.5185270009273468 32-33 0.5206357694167735 34-35 0.532508775032999 36-37 0.5509636944192529 38-39 0.541442285179115 40-41 0.5458643087993669 42-43 0.5433849082724048 44-45 0.5532769495500782 46-47 0.5515132522680125 48-49 0.5237030690377574 50-51 0.5387967392560162 52-53 0.5315885851467038 54-55 0.5404070715570328 56-57 0.5287129917520313 58-59 0.5345536414469884 60-61 0.5279589472618728 62-63 0.5220927367367408 64-65 0.5331989174477204 66-67 0.5314607809958295 68-69 0.5517049584943239 70-71 0.5365729470308028 72-73 0.5395635641612623 74-75 0.5367774336722018 76-77 0.52513447552755 78-79 0.5557946913223026 80-81 0.529939911600425 82-83 0.5286363092615067 84-85 0.5383110834826937 86-87 0.8462296241842261 88-89 1.114145465662092 90-91 1.104291765629681 92-93 1.1039594748374075 94-95 1.1087393500801077 96-97 1.1028731395549758 98-99 1.0977354126898275 100 1.094208018125696 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3912236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.06032199267275 #Duplication Level Percentage of deduplicated Percentage of total 1 75.47434786606566 31.744753734337145 2 12.71528863101414 10.696182681004515 3 4.707123980465191 5.93949450833392 4 2.322053851828954 3.906653307690072 5 1.2573358842648283 2.644197607256029 6 0.7660749562134762 1.933281559731689 7 0.5225468148948084 1.538494110350484 8 0.34102974183442764 1.1475056600507267 9 0.25364851368007896 0.9601684342512282 >10 1.3584787029580274 10.270936379889086 >50 0.1174776165537349 3.442423683381811 >100 0.1335024985421829 11.891360515315242 >500 0.021781912184085137 6.211832837257216 >1k 0.00888312619009816 6.32013352410723 >5k 3.6505998041499283E-4 1.0445949690669765 >10k+ 6.084333006916547E-5 0.3079864879767035 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12035 0.30762459115452134 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9361 0.23927493126692767 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7705 0.19694619649734832 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7016 0.17933478450686513 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6047 0.15456634006741923 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5406 0.13818184792532964 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5284 0.13506342664399593 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 4333 0.11075507714769764 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4284 0.10950259646912916 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3969 0.10145093496404614 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2780415087433375E-4 2 0.0 0.0 0.0 0.0 1.2780415087433375E-4 3 0.0 0.0 0.0 0.0 1.2780415087433375E-4 4 0.0 0.0 0.0 0.0 1.2780415087433375E-4 5 0.0 0.0 0.0 0.0 1.533649810492005E-4 6 0.0 0.0 0.0 0.0 2.04486641398934E-4 7 0.0 0.0 0.0 0.0 2.04486641398934E-4 8 0.0 0.0 0.0 0.0 2.04486641398934E-4 9 0.0 0.0 0.0 0.0 2.04486641398934E-4 10-11 0.0 0.0 0.0 0.0 2.3004747157380078E-4 12-13 0.0 0.0 0.0 0.0 4.4731452806016816E-4 14-15 0.0 0.0 0.0 0.0 5.367774336722018E-4 16-17 0.0 0.0 0.0 1.2780415087433375E-5 6.006795091093686E-4 18-19 0.0 0.0 0.0 2.556083017486675E-5 6.13459924196802E-4 20-21 0.0 0.0 0.0 3.8341245262300125E-5 7.157032448962691E-4 22-23 0.0 0.0 0.0 8.946290561203362E-5 7.412640750711358E-4 24-25 0.0 0.0 0.0 1.1502373578690037E-4 7.412640750711358E-4 26-27 0.0 0.0 0.0 2.811691319235343E-4 7.923857354208693E-4 28-29 0.0 0.0 0.0 0.0011118961126067037 7.923857354208693E-4 30-31 0.0 0.0 0.0 0.005022703129361317 8.051661505083026E-4 32-33 0.0 0.0 0.0 0.014429088633712282 8.946290561203363E-4 34-35 0.0 0.0 0.0 0.0307496787003647 8.946290561203363E-4 36-37 0.0 0.0 0.0 0.05780581744046116 9.074094712077697E-4 38-39 0.0 0.0 0.0 0.10191102990719374 9.201898862952031E-4 40-41 0.0 0.0 0.0 0.15273874070991628 9.201898862952031E-4 42-43 0.0 0.0 0.0 0.20616087577538778 9.457507164700699E-4 44-45 0.0 0.0 0.0 0.2724784496640795 0.0010352136220821034 46-47 0.0 0.0 0.0 0.35943639391897625 0.0011118961126067037 48-49 0.0 0.0 0.0 0.46143688673178207 0.0011630177729564373 50-51 0.0 0.0 0.0 0.5706838748991625 0.0012013590182187372 52-53 0.0 0.0 0.0 0.6806465663114393 0.0012524806785684708 54-55 0.0 1.2780415087433375E-5 0.0 0.8030318211887013 0.0012780415087433375 56-57 0.0 2.556083017486675E-5 0.0 0.9552337844649454 0.001290821923830771 58-59 0.0 2.556083017486675E-5 0.0 1.1360510971219528 0.0013163827540056377 60-61 0.0 2.556083017486675E-5 0.0 1.3298916527530547 0.0013547239992679379 62-63 0.0 2.556083017486675E-5 0.0 1.5334581042656934 0.0014569673199674048 64-65 0.0 2.556083017486675E-5 0.0 1.7486291726777219 0.0014569673199674048 66-67 0.0 2.556083017486675E-5 0.0 1.9932463174511967 0.0014825281501422715 68-69 0.0 2.556083017486675E-5 0.0 2.2754634434119003 0.0015080889803171383 70-71 0.0 2.556083017486675E-5 0.0 2.586577087885291 0.0015080889803171383 72-73 0.0 2.556083017486675E-5 0.0 2.91094402280435 0.0015847714708417384 74-75 0.0 2.556083017486675E-5 0.0 3.2358732959872567 0.0016742343764537722 76-77 0.0 2.556083017486675E-5 0.0 3.600894731299441 0.001699795206628639 78-79 0.0 2.556083017486675E-5 0.0 4.013472091151965 0.0017253560368035057 80-81 0.0 2.556083017486675E-5 0.0 4.463917820908555 0.0017509168669783725 82-83 0.0 2.556083017486675E-5 0.0 4.930517995335659 0.0018659406027652728 84-85 0.0 2.556083017486675E-5 0.0 5.389258214483993 0.0018787210178527062 86-87 0.0 2.556083017486675E-5 0.0 5.875284619843997 0.002032085998901907 88 0.0 2.556083017486675E-5 0.0 6.268435748763623 0.002172670564863674 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 2750 0.0 32.931408 1 GTATCAA 8730 0.0 30.202253 1 CAACGCA 8350 0.0 29.485806 5 GGGCACC 3485 0.0 28.31322 94 ATCAACG 8755 0.0 28.123621 3 TAGGCAT 3790 0.0 28.018017 5 GCATCGT 3250 0.0 26.582138 8 GTACATG 15105 0.0 26.105217 1 AACGCAG 9930 0.0 26.04932 6 CTAGGCA 4145 0.0 25.958471 4 TAGTACT 4115 0.0 25.919355 4 TATCAAC 10260 0.0 25.46513 2 GGTATCA 5935 0.0 25.431444 1 TACATGG 15285 0.0 25.271141 2 ACGCAGA 9935 0.0 24.71631 7 ACATGGG 15175 0.0 24.64793 3 CTGTGCG 4375 0.0 23.612953 9 TCAACGC 10765 0.0 23.438423 4 CGCAGAG 10320 0.0 23.248592 8 CTAGTAC 4635 0.0 22.911568 3 >>END_MODULE