FastQCFastQC Report
Mon 16 Jan 2017
ERR1146460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912236
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC105280.26910442008099716No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT78190.19986013113728313No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA77590.19832648132679112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71070.181660820052778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66290.1694427432291917No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC62680.1602152835360648No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61440.1570457405943813No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA58580.14973534316436943No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG47440.12126057834956788No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC47140.12049375344432188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.039.0545121
GTATCAA128750.036.6639671
GTACTAG25950.031.1633431
CAACGCA150800.030.4171315
ATCAACG155650.029.4172273
GGGCACC36350.028.46565494
TATCAAC165700.028.3717442
AACGCAG169450.027.7350036
TCAACGC175300.026.4951824
TAGTACT43000.025.8009344
CTGTGCG44150.025.3398939
ACGCAGA181700.025.2961087
TAGGCAT37850.024.8332675
CGCAGAG186650.024.574898
TACCCCG3450.024.5201535
CTAGTAC46800.024.5095793
GTACATG149150.023.768461
CTAACGC15200.023.5050893
CAGTACG6500.023.1416959
TAACGCC15850.023.1343464
Produced by FastQC (version 0.11.5)