##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3912236
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880387072763504	31.0	31.0	34.0	30.0	34.0
2	31.83293620323518	31.0	31.0	34.0	30.0	34.0
3	32.02003227821635	33.0	31.0	34.0	30.0	34.0
4	35.750721326627534	37.0	35.0	37.0	35.0	37.0
5	35.658173484421695	37.0	35.0	37.0	33.0	37.0
6	35.6960035130805	37.0	35.0	37.0	33.0	37.0
7	35.612488612650154	37.0	35.0	37.0	33.0	37.0
8	35.59189450738657	37.0	35.0	37.0	33.0	37.0
9	37.19014803810404	39.0	37.0	39.0	33.0	39.0
10-11	37.14233279894158	39.0	37.0	39.0	33.0	39.0
12-13	37.08843932216769	39.0	37.0	39.0	33.0	39.0
14-15	38.306187433477945	40.0	38.0	41.0	33.0	41.0
16-17	38.29594265274385	40.0	38.0	41.0	33.0	41.0
18-19	38.345764417075046	40.0	38.0	41.0	33.0	41.0
20-21	38.35839223400633	40.0	38.0	41.0	33.0	41.0
22-23	38.33071471148469	40.0	38.0	41.0	33.0	41.0
24-25	38.270363929987866	40.0	38.0	41.0	33.0	41.0
26-27	38.14011718618202	40.0	37.0	41.0	33.0	41.0
28-29	38.070248957373735	40.0	37.0	41.0	33.0	41.0
30-31	37.955642118726985	40.0	37.0	41.0	33.0	41.0
32-33	37.8482498243971	40.0	37.0	41.0	33.0	41.0
34-35	37.75993063813124	40.0	37.0	41.0	32.5	41.0
36-37	37.679732383220234	40.0	36.5	41.0	32.0	41.0
38-39	37.5801545458914	40.0	36.0	41.0	32.0	41.0
40-41	37.463524950948766	40.0	36.0	41.0	32.0	41.0
42-43	37.34741027893	40.0	36.0	41.0	31.0	41.0
44-45	37.181609059371674	39.0	36.0	41.0	31.0	41.0
46-47	36.976049374322	39.0	35.0	41.0	31.0	41.0
48-49	36.747351131168976	39.0	35.0	41.0	30.0	41.0
50-51	36.567224472143295	39.0	35.0	41.0	30.0	41.0
52-53	36.391739787681516	39.0	35.0	41.0	30.0	41.0
54-55	36.196964344686776	38.0	35.0	40.5	29.5	41.0
56-57	35.96761315012694	38.0	35.0	40.0	29.0	41.0
58-59	35.708409717614174	37.5	34.0	40.0	29.0	41.0
60-61	35.44894633145853	37.0	34.0	40.0	28.0	41.0
62-63	35.17992830698353	36.5	34.0	40.0	28.0	41.0
64-65	34.89700774697641	36.0	34.0	39.0	28.0	41.0
66-67	34.6562961692495	36.0	34.0	39.0	28.0	41.0
68-69	34.44375173174625	35.0	33.5	39.0	28.0	41.0
70-71	34.190472021626505	35.0	33.0	38.0	27.5	40.0
72-73	33.95970475707498	35.0	33.0	37.0	27.5	40.0
74-75	33.757827109611995	35.0	33.0	37.0	27.0	39.0
76-77	33.578531944391905	35.0	33.0	36.5	27.0	39.0
78-79	33.444847907948294	35.0	33.0	36.0	28.0	39.0
80-81	33.272285721004565	35.0	33.0	36.0	27.5	37.5
82-83	33.083400387911155	35.0	33.0	35.0	27.0	37.0
84-85	32.93479828415259	35.0	33.0	35.0	27.0	37.0
86-87	32.79588207357634	35.0	33.0	35.0	27.0	36.0
88-89	32.683005191915825	35.0	33.0	35.0	27.0	36.0
90-91	32.59300129133314	35.0	33.0	35.0	27.0	36.0
92-93	32.51989476095	35.0	33.0	35.0	27.0	36.0
94-95	32.46997854935132	35.0	33.0	35.0	27.0	35.0
96-97	32.42213979422509	35.0	33.0	35.0	27.0	35.0
98-99	32.37921561991659	35.0	33.0	35.0	27.0	35.0
100	31.8788807730413	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43856394839004054
1101	2	0.5613999174652129
1101	3	0.4385878359494484
1101	4	0.3687631381849812
1101	5	0.3365841827946099
1101	6	0.3607222148807949
1101	7	0.40122046002933587
1101	8	0.4153817588612867
1101	9	0.4301386614433085
1101	10-11	0.4788945951392449
1101	12-13	0.5196045216341503
1101	14-15	0.6111841810753873
1101	16-17	0.5811000874311318
1101	18-19	0.6664141707255951
1101	20-21	0.6136512536279568
1101	22-23	0.5930251190343085
1101	24-25	0.614071194494727
1101	26-27	0.6314070588663867
1101	28-29	0.7128416167942433
1101	30-31	0.752547688611628
1101	32-33	0.77354092298485
1101	34-35	0.6979280595051947
1101	36-37	0.7330772517501174
1101	38-39	0.7806947481863915
1101	40-41	0.8500250441891097
1101	42-43	0.7992632992333668
1101	44-45	0.8272976736785296
1101	46-47	0.7536572387718934
1101	48-49	0.7481426496870824
1101	50-51	0.7659301194248656
1101	52-53	0.8253232687986838
1101	54-55	0.7672821430651595
1101	56-57	0.7481602945218668
1101	58-59	0.7828257741545102
1101	60-61	0.7789677186300707
1101	62-63	0.7534332901061163
1101	64-65	0.7782267136645018
1101	66-67	0.6782374260562634
1101	68-69	0.6164374388475835
1101	70-71	0.6605043713104095
1101	72-73	0.5985169483871573
1101	74-75	0.5644507212361916
1101	76-77	0.5665873331688189
1101	78-79	0.5140765499809063
1101	80-81	0.527022888968709
1101	82-83	0.5450368144500715
1101	84-85	0.5332052081385825
1101	86-87	0.5738715896696007
1101	88-89	0.5303819646869101
1101	90-91	0.5093074361450576
1101	92-93	0.5162232456176028
1101	94-95	0.5749610986875311
1101	96-97	0.5620098484562916
1101	98-99	0.5718816314017872
1101	100	0.6313470771670637
1102	1	0.36822439248696526
1102	2	0.49832117630613126
1102	3	0.43448144011856726
1102	4	0.2634343180438705
1102	5	0.3061480858283545
1102	6	0.32457911863212985
1102	7	0.30265176199817745
1102	8	0.3682648263576098
1102	9	0.37897245696453297
1102	10-11	0.3885540761081572
1102	12-13	0.4568878997188719
1102	14-15	0.5386355594882986
1102	16-17	0.6100798317835299
1102	18-19	0.6056464854986459
1102	20-21	0.5847928174025299
1102	22-23	0.537058953010785
1102	24-25	0.5860527875695993
1102	26-27	0.6077038031472597
1102	28-29	0.6077529817949809
1102	30-31	0.6447778281070953
1102	32-33	0.6466252102822025
1102	34-35	0.6935680127917365
1102	36-37	0.7281535655238258
1102	38-39	0.6773425968807345
1102	40-41	0.6218683899630335
1102	42-43	0.7516170684950936
1102	44-45	0.6369345059994203
1102	46-47	0.695836891202056
1102	48-49	0.6617613495801464
1102	50-51	0.6499162267400891
1102	52-53	0.6168284011674956
1102	54-55	0.6503732080701496
1102	56-57	0.7114549271707773
1102	58-59	0.614779502317333
1102	60-61	0.586988982330297
1102	62-63	0.5473458639955453
1102	64-65	0.5437680491067027
1102	66-67	0.5761994582575269
1102	68-69	0.6132146867593846
1102	70-71	0.5363746257395405
1102	72-73	0.5459488802324017
1102	74-75	0.49290294628752207
1102	76-77	0.5070067876441442
1102	78-79	0.47239128967387956
1102	80-81	0.46106373124452915
1102	82-83	0.48702781549206975
1102	84-85	0.46082650236309775
1102	86-87	0.5200146390429268
1102	88-89	0.46910630804717357
1102	90-91	0.48832718612133164
1102	92-93	0.4612596731394092
1102	94-95	0.46561832320591634
1102	96-97	0.44633025552286654
1102	98-99	0.5134458630869432
1102	100	0.5217172848809852
1103	1	0.27013314983199876
1103	2	-0.13955483326270013
1103	3	0.19629281525613607
1103	4	0.19034207240827783
1103	5	0.21216499935935929
1103	6	0.23461870555620123
1103	7	0.2937510628485711
1103	8	0.27766643894067755
1103	9	0.3222242066740648
1103	10-11	0.39664032877065125
1103	12-13	0.3844640456144006
1103	14-15	0.43358061581647434
1103	16-17	0.4482215722184435
1103	18-19	0.44014550519980844
1103	20-21	0.4256296257687211
1103	22-23	0.44730162712231447
1103	24-25	0.4355759325602122
1103	26-27	0.4354682996260735
1103	28-29	0.44467790351660597
1103	30-31	0.41331533551800703
1103	32-33	0.45835040775138935
1103	34-35	0.4485457466219245
1103	36-37	0.4528220115961119
1103	38-39	0.44182675149130546
1103	40-41	0.5328051528869935
1103	42-43	0.48814769427895754
1103	44-45	0.47927893190511384
1103	46-47	0.44397757490182244
1103	48-49	0.4832327654190607
1103	50-51	0.4497587915595176
1103	52-53	0.4586965096089699
1103	54-55	0.4149154437808278
1103	56-57	0.4662091208753125
1103	58-59	0.36887421987405844
1103	60-61	0.41088223582374894
1103	62-63	0.4339699833064472
1103	64-65	0.4104544869469464
1103	66-67	0.5125763985661536
1103	68-69	0.4099851153769194
1103	70-71	0.32456032688067893
1103	72-73	0.307034962715953
1103	74-75	0.3628379924757681
1103	76-77	0.3960443536605993
1103	78-79	0.39696793351978243
1103	80-81	0.3319561322700153
1103	82-83	0.30657154190727454
1103	84-85	0.284864237509602
1103	86-87	0.32589422944923996
1103	88-89	0.33932035328959387
1103	90-91	0.3603303390204218
1103	92-93	0.3948501628378125
1103	94-95	0.3784270544078936
1103	96-97	0.36802133067352116
1103	98-99	0.3938574024248922
1103	100	0.43884276601669114
1104	1	0.2844842685639577
1104	2	0.34596180226100515
1104	3	0.2889107106249327
1104	4	0.2239720121802833
1104	5	0.22315470086228117
1104	6	0.19353631370327662
1104	7	0.23077457560273018
1104	8	0.19402405859655403
1104	9	0.24035670265794806
1104	10-11	0.2792720048017756
1104	12-13	0.29557833645239384
1104	14-15	0.3754055015831099
1104	16-17	0.34010580171451466
1104	18-19	0.3221956642692234
1104	20-21	0.3089287092758397
1104	22-23	0.3269454674011669
1104	24-25	0.3789719852047142
1104	26-27	0.41289924818465096
1104	28-29	0.44041584540792655
1104	30-31	0.4076747157108329
1104	32-33	0.4296396162101175
1104	34-35	0.42259543016040624
1104	36-37	0.4045444603192294
1104	38-39	0.45901383102817306
1104	40-41	0.4049967305278699
1104	42-43	0.419046939401305
1104	44-45	0.4229232130905487
1104	46-47	0.4442474582279914
1104	48-49	0.46338227845015467
1104	50-51	0.4981918609172311
1104	52-53	0.5096431224610214
1104	54-55	0.47963499551336497
1104	56-57	0.48457870324077135
1104	58-59	0.42539597813886587
1104	60-61	0.4191800260184948
1104	62-63	0.45913043929036235
1104	64-65	0.358809733145101
1104	66-67	0.4039032432164973
1104	68-69	0.40620233333275024
1104	70-71	0.4077606348391214
1104	72-73	0.40258435642131474
1104	74-75	0.3750062476506244
1104	76-77	0.27689458115096244
1104	78-79	0.31626257450253803
1104	80-81	0.34509854675758334
1104	82-83	0.291739354689156
1104	84-85	0.31886000617039656
1104	86-87	0.3374576807533529
1104	88-89	0.35589846121104074
1104	90-91	0.29025715896158033
1104	92-93	0.30203333780996644
1104	94-95	0.29828151384951695
1104	96-97	0.2394172625408615
1104	98-99	0.26732847767941337
1104	100	0.33125844124830905
1105	1	0.11548168402112324
1105	2	-0.4007552082948713
1105	3	-0.0130991378773615
1105	4	0.09011340550695479
1105	5	0.11422588099994613
1105	6	0.18891855740444896
1105	7	0.19790835892644765
1105	8	0.2174677167873682
1105	9	0.2957688891879542
1105	10-11	0.3079835916004825
1105	12-13	0.32984997841730745
1105	14-15	0.35197081998887114
1105	16-17	0.3773982118242927
1105	18-19	0.3795798341243213
1105	20-21	0.3934210392902955
1105	22-23	0.35357461285011027
1105	24-25	0.4408305582659864
1105	26-27	0.39670139550700867
1105	28-29	0.40871420591142993
1105	30-31	0.3858747784667145
1105	32-33	0.49976418641970355
1105	34-35	0.44405074352980733
1105	36-37	0.41086745449657514
1105	38-39	0.44170557622165063
1105	40-41	0.4819599186524641
1105	42-43	0.4155845255726902
1105	44-45	0.32766331208377153
1105	46-47	0.38936797391252753
1105	48-49	0.3480837746365779
1105	50-51	0.42838177271973166
1105	52-53	0.40809894422699955
1105	54-55	0.3570265737851699
1105	56-57	0.36759703021023427
1105	58-59	0.3968109814273646
1105	60-61	0.407940767857049
1105	62-63	0.3585631183865843
1105	64-65	0.30028116194309007
1105	66-67	0.33719138574328866
1105	68-69	0.38291268256565303
1105	70-71	0.28653466634250435
1105	72-73	0.338210988394529
1105	74-75	0.30054821827283007
1105	76-77	0.30417022796851967
1105	78-79	0.26482815308620644
1105	80-81	0.2952008727887758
1105	82-83	0.2631488659065866
1105	84-85	0.26723971471435704
1105	86-87	0.26852592241790063
1105	88-89	0.23772846460465047
1105	90-91	0.2434828100365678
1105	92-93	0.30017762005206094
1105	94-95	0.31470885766398027
1105	96-97	0.22762962896988626
1105	98-99	0.2094585391570547
1105	100	0.24391383351998286
1106	1	0.2143667357840897
1106	2	0.29648844312855616
1106	3	0.2429632568041029
1106	4	0.1823104210057096
1106	5	0.16890686536444832
1106	6	0.1986264466343144
1106	7	0.21656554028979969
1106	8	0.21404863178671008
1106	9	0.24982054329061754
1106	10-11	0.25191451681116206
1106	12-13	0.23035143072223718
1106	14-15	0.27805756332607956
1106	16-17	0.3154034793255036
1106	18-19	0.3176463640624476
1106	20-21	0.2891992954717537
1106	22-23	0.350861381333317
1106	24-25	0.3215027739205709
1106	26-27	0.3024835770952805
1106	28-29	0.27533731973687026
1106	30-31	0.3163030717823716
1106	32-33	0.29162254069304794
1106	34-35	0.2481549641209142
1106	36-37	0.3136257064540473
1106	38-39	0.290945157277676
1106	40-41	0.33163625112031525
1106	42-43	0.291181433694895
1106	44-45	0.3268018846234355
1106	46-47	0.32685776819219825
1106	48-49	0.27751639169141384
1106	50-51	0.3356082324245051
1106	52-53	0.2786321000538976
1106	54-55	0.2860298617702455
1106	56-57	0.3721649475640305
1106	58-59	0.331671453204919
1106	60-61	0.2624931432764157
1106	62-63	0.2684042808288041
1106	64-65	0.23869108766118785
1106	66-67	0.3226179685464885
1106	68-69	0.23718298087182887
1106	70-71	0.3395843801774845
1106	72-73	0.3337272049748776
1106	74-75	0.32570838341769814
1106	76-77	0.2673182012417925
1106	78-79	0.286536198185253
1106	80-81	0.23431412164686094
1106	82-83	0.20811232605401386
1106	84-85	0.16136094708854642
1106	86-87	0.2008582292188379
1106	88-89	0.1731399468778534
1106	90-91	0.16402918269302091
1106	92-93	0.2483954398133008
1106	94-95	0.2502060623649811
1106	96-97	0.2375500670859978
1106	98-99	0.29601096192195797
1106	100	0.2887449482478033
1107	1	0.01870542150328447
1107	2	-0.4630734990754988
1107	3	-0.06351722724308928
1107	4	0.008306474234935024
1107	5	0.09890424134499654
1107	6	0.12480595104757697
1107	7	0.17408558104649785
1107	8	0.13289622689590885
1107	9	0.09109038456045937
1107	10-11	0.2024536684089
1107	12-13	0.17889264470719013
1107	14-15	0.19673751274849138
1107	16-17	0.23968958491236236
1107	18-19	0.2304585226330218
1107	20-21	0.15764039774406058
1107	22-23	0.1683213151650591
1107	24-25	0.11447953216704576
1107	26-27	0.20839233947880587
1107	28-29	0.1327044077811621
1107	30-31	0.17757434782308223
1107	32-33	0.16715731892503527
1107	34-35	0.19936297213721588
1107	36-37	0.24169132075081734
1107	38-39	0.21633323594301856
1107	40-41	0.22542350145484136
1107	42-43	0.15941077299441275
1107	44-45	0.1971279458935271
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2216	74-75	-0.9046174558939057
2216	76-77	-0.8051909897616127
2216	78-79	-0.7366719680389267
2216	80-81	-0.688231080202165
2216	82-83	-0.6383817356869343
2216	84-85	-0.6992167680821382
2216	86-87	-0.6585064735622339
2216	88-89	-0.6147697059116979
2216	90-91	-0.642908259642553
2216	92-93	-0.6644044740431099
2216	94-95	-0.5583456896921106
2216	96-97	-0.6369703767182529
2216	98-99	-0.6750200191735232
2216	100	-0.6790253449228452
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	8.0
13	42.0
14	188.0
15	553.0
16	1345.0
17	2684.0
18	4811.0
19	7730.0
20	10897.0
21	16008.0
22	22412.0
23	27363.0
24	30527.0
25	33138.0
26	38243.0
27	47012.0
28	59062.0
29	73395.0
30	92086.0
31	112938.0
32	140983.0
33	177772.0
34	240610.0
35	331547.0
36	500631.0
37	855837.0
38	817537.0
39	266343.0
40	532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.05569523497885	20.267962937162153	13.946982604905495	24.729359222953498
2	15.125180020889326	19.96632963927316	39.68509061498766	25.22339972484986
3	18.322655976614534	25.444605849485697	29.711722871151146	26.521015302748623
4	12.752884169424147	16.884836230925824	37.52534402678682	32.83693557286321
5	13.198053753120092	38.09176295868379	34.73444874870435	13.975734539491762
6	31.028521796742325	37.64432922758238	17.300873464688735	14.026275510986554
7	27.123272334656367	32.015716923605446	22.745369887552222	18.115640854185965
8	26.459702328796116	34.95504872405448	19.38898880333395	19.196260143815454
9	26.10006144823574	15.021077460562196	20.601696830150328	38.27716426105174
10-11	25.724892363344132	25.750172024387076	27.244905470937848	21.28003014133094
12-13	26.134760275198122	23.333855626296575	27.725091226602892	22.80629287190241
14-15	23.39417863192597	24.5319843948572	25.306707161747234	26.767129811469598
16-17	22.419337543411558	27.329078948360614	26.346670558843982	23.904912949383846
18-19	22.073732259505817	26.875270305779097	28.646686958557716	22.40431047615737
20-21	23.286436434216814	26.060055960346958	28.214119066737947	22.43938853869828
22-23	23.532421947589228	25.677947840747855	27.996419416028427	22.79321079563449
24-25	23.047280378791054	25.861719487270197	28.46804231646557	22.62295781747318
26-27	22.984781058373358	26.105175309222616	27.760318283945473	23.149725348458553
28-29	22.446651991342033	26.425834228814416	28.225444477275914	22.902069302567636
30-31	23.07344188847503	26.056454671957418	28.087939991350215	22.78216344821734
32-33	23.302326342275876	26.22856852193988	27.52309676614601	22.946008369638232
34-35	23.285087130724843	26.1585670558314	28.012051965313855	22.5442938481299
36-37	23.16207406710638	25.827429633590608	27.79925597535527	23.211240323947738
38-39	23.239881234158673	25.89144673276357	28.212178406415156	22.656493626662606
40-41	22.867820760499544	25.424452318352404	28.586367119623702	23.121359801524353
42-43	23.029348053774836	25.54041503230342	27.977521096497714	23.452715817424025
44-45	23.252878916302596	26.043124699021224	27.713243781816843	22.990752602859338
46-47	23.276944437912235	26.07030864191219	27.15700177596648	23.49574514420909
48-49	23.816631590597797	25.990287079315628	27.063196864484446	23.129884465602128
50-51	23.403524660005772	26.122698901944936	26.91964734740534	23.554129090643954
52-53	23.088599460768727	26.37158136676826	26.807214595438516	23.732604577024492
54-55	23.046705109494837	26.32349206645389	26.759050783615884	23.870752040435388
56-57	23.713720527381675	25.935124518401636	26.621842308471916	23.72931264574477
58-59	22.98231427729014	26.187703744430756	27.17165826543397	23.65832371284514
60-61	22.998741255470183	26.534332132441552	26.87540346219287	23.591523149895398
62-63	23.2665028387858	26.16121573442911	26.529790125135598	24.04249130164949
64-65	22.706554512560082	26.21747512164399	26.83763198334661	24.23833838244932
66-67	23.163454351935826	25.951310197033102	26.815969179773408	24.069266271257664
68-69	22.869798580176713	25.908515897925888	27.019457452973118	24.202228068924285
70-71	22.803748637753056	25.659882196552342	27.087354680346287	24.449014485348314
72-73	23.16456624804843	25.713441111425794	27.28567499506676	23.836317645459015
74-75	23.201064560856505	26.14174522493411	26.89525534520203	23.761934869007355
76-77	23.104650461334597	25.99645257005906	26.537794775631063	24.36110219297528
78-79	22.902593213251222	25.993900637286526	26.74805199718216	24.35545415228009
80-81	22.615686105602215	26.366083430009745	27.03255318651299	23.985677277875048
82-83	23.213033209275693	25.63608083513967	27.670340681362728	23.480545274221914
84-85	23.30573241487906	25.953107196241316	26.738356202026708	24.002804186852913
86-87	22.335349263514672	26.459789804225515	27.09057907431496	24.114281857944857
88-89	22.959229190828797	25.565173401132586	27.006197179354185	24.469400228684428
90-91	22.75053971760477	25.586813086249116	27.072293848708313	24.590353347437798
92-93	22.918125358681664	25.819430224993255	26.895264315632193	24.367180100692888
94-95	22.12805169509505	25.70412328707597	27.368123062136867	24.79970195569211
96-97	22.295620823719336	25.572267317238012	27.160974614706333	24.97113724433631
98-99	21.926367057396906	26.029354101389462	27.01795068813613	25.026328153077504
100	22.46847262613508	25.452419349887336	26.571277856343233	25.507830167634353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	127.5
2	148.0
3	196.5
4	142.5
5	117.0
6	255.5
7	675.5
8	1183.5
9	1384.5
10	1161.0
11	845.0
12	801.0
13	908.0
14	1035.5
15	1244.0
16	1526.0
17	1878.5
18	2393.5
19	2929.5
20	3568.0
21	4290.0
22	5098.5
23	6182.0
24	7217.0
25	8326.5
26	10235.5
27	12625.0
28	15744.5
29	21321.0
30	29049.0
31	36630.0
32	46292.5
33	56101.5
34	63695.0
35	72469.5
36	82213.5
37	98269.0
38	107520.5
39	104598.5
40	102264.0
41	100547.0
42	102745.0
43	110891.5
44	121785.5
45	140979.0
46	159133.0
47	171685.0
48	182875.0
49	182618.5
50	175767.0
51	176643.0
52	176452.0
53	171008.5
54	165494.5
55	148999.5
56	132727.5
57	113644.0
58	94342.5
59	87044.5
60	73613.5
61	55689.5
62	43002.5
63	34198.0
64	24797.0
65	15188.0
66	10123.0
67	7363.5
68	5475.5
69	4055.5
70	3036.0
71	2304.0
72	1664.0
73	1234.0
74	889.5
75	583.0
76	395.5
77	239.5
78	127.0
79	59.5
80	29.5
81	18.0
82	11.0
83	4.0
84	2.0
85	0.5
86	1.0
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012805975917608244
2	0.004856557733224683
3	0.0016103323010166054
4	0.027043358325009027
5	0.002326035545912874
6	0.0
7	5.11216603497335E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008192246071044794
16-17	0.00295227588519711
18-19	0.0
20-21	3.8341245262300125E-5
22-23	6.390207543716688E-4
24-25	0.0
26-27	1.533649810492005E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.811691319235343E-4
36-37	0.0
38-39	0.0
40-41	4.6009494314760155E-4
42-43	8.818486410329029E-4
44-45	0.0
46-47	0.0
48-49	6.262403392842354E-4
50-51	1.2780415087433375E-5
52-53	0.0
54-55	4.984361884099017E-4
56-57	7.668249052460025E-5
58-59	3.195103771858344E-4
60-61	1.1502373578690039E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015080889803171383
70-71	0.0010607744522569702
72-73	0.0
74-75	3.195103771858344E-4
76-77	0.002760569658885609
78-79	6.13459924196802E-4
80-81	0.003335688337820111
82-83	0.0019426230932898729
84-85	0.005840649694957053
86-87	0.007962198599470992
88-89	0.007540444901585691
90-91	0.006045136336355986
92-93	0.008716243089629561
94-95	0.0016870147915412055
96-97	0.004792655657787516
98-99	0.0018915014329401395
100	0.08312381972866668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3912236.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.2131109522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60693482846078	24.158636082049433
2	15.950594875018206	11.233401341752174
3	6.159388865706317	6.506737305775853
4	2.9826414713194307	4.201123402408166
5	1.6022485741187424	2.821007840672375
6	1.040055894711812	2.1974162170186
7	0.6952114517780028	1.7136390590689192
8	0.46921275741234525	1.3217952709558891
9	0.3529417314683073	1.1185358714869573
>10	1.7665744760995399	11.318742972117235
>50	0.15662037400513734	3.8399749182000806
>100	0.17443351294566606	13.096472592850414
>500	0.029922843479239646	7.245738803760416
>1k	0.012637317389666508	7.740583094233122
>5k	5.083978260210663E-4	1.2169451010552519
>10k+	7.262826086015234E-5	0.26925012659527764
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10528	0.26910442008099716	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7819	0.19986013113728313	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7759	0.19832648132679112	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7107	0.181660820052778	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6629	0.1694427432291917	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6268	0.1602152835360648	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6144	0.1570457405943813	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5858	0.14973534316436943	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4744	0.12126057834956788	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4714	0.12049375344432188	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.668249052460025E-5
2	0.0	0.0	0.0	0.0	7.668249052460025E-5
3	0.0	0.0	0.0	0.0	7.668249052460025E-5
4	0.0	0.0	0.0	0.0	7.668249052460025E-5
5	0.0	0.0	0.0	0.0	7.668249052460025E-5
6	0.0	0.0	0.0	0.0	7.668249052460025E-5
7	0.0	0.0	0.0	0.0	7.668249052460025E-5
8	0.0	0.0	0.0	0.0	7.668249052460025E-5
9	0.0	0.0	0.0	0.0	7.668249052460025E-5
10-11	0.0	0.0	0.0	0.0	1.2780415087433375E-4
12-13	0.0	0.0	0.0	0.0	3.834124526230013E-4
14-15	0.0	0.0	0.0	0.0	4.856557733224683E-4
16-17	0.0	0.0	0.0	1.2780415087433375E-5	5.878990940219352E-4
18-19	0.0	0.0	0.0	2.556083017486675E-5	7.15703244896269E-4
20-21	0.0	0.0	0.0	3.8341245262300125E-5	8.051661505083026E-4
22-23	0.0	0.0	0.0	8.946290561203362E-5	8.690682259454695E-4
24-25	0.0	0.0	0.0	1.1502373578690037E-4	8.690682259454695E-4
26-27	0.0	0.0	0.0	3.06729962098401E-4	9.074094712077697E-4
28-29	0.0	0.0	0.0	0.0012013590182187372	9.457507164700698E-4
30-31	0.0	0.0	0.0	0.00507382478971105	9.457507164700698E-4
32-33	0.0	0.0	0.0	0.014544112369499183	9.713115466449365E-4
34-35	0.0	0.0	0.0	0.03108196949263797	0.0010735548673444035
36-37	0.0	0.0	0.0	0.058214790723259024	0.0010735548673444035
38-39	0.0	0.0	0.0	0.10258839190682771	0.0011374569427815705
40-41	0.0	0.0	0.0	0.1541318059544465	0.0011502373578690039
42-43	0.0	0.0	0.0	0.2088192021135739	0.0011502373578690039
44-45	0.0	0.0	0.0	0.2760186246432986	0.0011757981880438704
46-47	0.0	0.0	0.0	0.36454855995394964	0.0012141394333061706
48-49	0.0	0.0	0.0	0.4675459251435752	0.0013036023389182043
50-51	0.0	0.0	0.0	0.5788505601400324	0.0013802848294428047
52-53	0.0	0.0	0.0	0.6905513880042001	0.0014569673199674048
54-55	0.0	0.0	0.0	0.8140357585789815	0.0014825281501422715
56-57	0.0	0.0	0.0	0.9680653212127285	0.0015464302255794385
58-59	0.0	0.0	0.0	1.151540960207922	0.0015847714708417387
60-61	0.0	0.0	0.0	1.347707551384937	0.0016231127161040388
62-63	0.0	0.0	0.0	1.5525648248214066	0.0017125756217160723
64-65	0.0	0.0	0.0	1.7703175370810964	0.0017636972820658059
66-67	0.0	0.0	0.0	2.0179764206453803	0.0017636972820658059
68-69	0.0	0.0	0.0	2.3023534367558605	0.0017636972820658059
70-71	0.0	0.0	0.0	2.617186182019694	0.0017892581122406727
72-73	0.0	0.0	0.0	2.9460007013891802	0.001993744753639607
74-75	0.0	0.0	0.0	3.2754286806828627	0.0021982313950385407
76-77	0.0	0.0	0.0	3.6473771009724363	0.002236572640300841
78-79	0.0	0.0	0.0	4.0684917781033665	0.002249353055388274
80-81	0.0	0.0	0.0	4.528637842911317	0.0022621334704757074
82-83	0.0	0.0	0.0	5.005078936955746	0.002338815961000308
84-85	0.0	0.0	0.0	5.473378906589479	0.0023515963760877413
86-87	0.0	0.0	0.0	5.969259011981895	0.0024282788666123416
88	0.0	0.0	0.0	6.369503271275046	0.0025049613571369415
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7765	0.0	39.054512	1
GTATCAA	12875	0.0	36.663967	1
GTACTAG	2595	0.0	31.163343	1
CAACGCA	15080	0.0	30.417131	5
ATCAACG	15565	0.0	29.417227	3
GGGCACC	3635	0.0	28.465654	94
TATCAAC	16570	0.0	28.371744	2
AACGCAG	16945	0.0	27.735003	6
TCAACGC	17530	0.0	26.495182	4
TAGTACT	4300	0.0	25.800934	4
CTGTGCG	4415	0.0	25.339893	9
ACGCAGA	18170	0.0	25.296108	7
TAGGCAT	3785	0.0	24.833267	5
CGCAGAG	18665	0.0	24.57489	8
TACCCCG	345	0.0	24.520153	5
CTAGTAC	4680	0.0	24.509579	3
GTACATG	14915	0.0	23.76846	1
CTAACGC	1520	0.0	23.505089	3
CAGTACG	650	0.0	23.141695	9
TAACGCC	1585	0.0	23.134346	4
>>END_MODULE