##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146459_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3997328 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.478598453767116 31.0 30.0 34.0 27.0 34.0 2 30.528067499089392 31.0 30.0 34.0 26.0 34.0 3 30.79222045326278 31.0 31.0 34.0 27.0 34.0 4 34.287336440742415 37.0 35.0 37.0 30.0 37.0 5 34.48540950354837 37.0 35.0 37.0 30.0 37.0 6 34.60784078764615 37.0 35.0 37.0 31.0 37.0 7 34.5373727149736 37.0 35.0 37.0 30.0 37.0 8 34.47085127865414 37.0 35.0 37.0 30.0 37.0 9 35.868836132536536 39.0 35.0 39.0 30.0 39.0 10-11 35.79508461652384 39.0 35.0 39.0 30.0 39.0 12-13 35.77352934260086 39.0 35.0 39.0 30.0 39.0 14-15 36.79318434714389 40.0 36.0 41.0 29.5 41.0 16-17 36.62088162392478 40.0 36.0 41.0 28.5 41.0 18-19 36.57523363106555 40.0 36.0 41.0 28.5 41.0 20-21 36.906838267962996 40.0 36.0 41.0 30.0 41.0 22-23 36.623848856035835 40.0 36.0 41.0 28.5 41.0 24-25 36.72746819875677 40.0 36.0 41.0 29.5 41.0 26-27 36.7904774139125 40.0 36.0 41.0 30.0 41.0 28-29 36.62483363887077 39.5 35.5 41.0 29.5 41.0 30-31 36.56698324480753 39.0 35.0 41.0 29.5 41.0 32-33 36.66423495895258 39.0 35.0 41.0 30.0 41.0 34-35 36.70690496251496 40.0 35.0 41.0 30.0 41.0 36-37 36.63976799001733 39.5 35.0 41.0 30.0 41.0 38-39 36.48020727846202 39.0 35.0 41.0 29.0 41.0 40-41 36.445441930209384 39.0 35.0 41.0 29.0 41.0 42-43 36.28643421305432 39.0 35.0 41.0 28.5 41.0 44-45 36.09626693131011 39.0 35.0 41.0 28.0 41.0 46-47 35.96369162100283 39.0 34.5 41.0 28.0 41.0 48-49 35.83270024376283 38.0 34.0 40.0 28.0 41.0 50-51 35.60673417342785 38.0 34.0 40.0 27.5 41.0 52-53 35.411259846577515 38.0 34.0 40.0 27.0 41.0 54-55 35.210501114744645 37.5 33.5 40.0 27.0 41.0 56-57 35.05768303226556 37.0 33.0 40.0 27.0 41.0 58-59 34.79310541942017 37.0 33.0 40.0 26.0 41.0 60-61 34.538563510424964 36.0 33.0 40.0 26.0 41.0 62-63 34.26144889786377 36.0 33.0 39.0 26.0 41.0 64-65 33.976143688984244 35.0 32.5 39.0 26.0 41.0 66-67 33.68318424207371 35.0 32.0 39.0 26.0 40.5 68-69 33.39875074049465 35.0 32.0 38.0 25.0 40.0 70-71 33.34634298211205 35.0 32.0 37.5 25.5 40.0 72-73 33.14228717283145 35.0 32.0 37.0 25.0 40.0 74-75 32.88262384272694 35.0 32.0 36.5 25.0 39.0 76-77 32.65322460403549 35.0 32.0 36.0 25.0 39.0 78-79 32.4375342728943 35.0 31.0 36.0 25.0 39.0 80-81 32.21844429578959 35.0 31.0 35.5 24.0 37.5 82-83 31.98184837471431 35.0 31.0 35.0 24.0 37.0 84-85 31.80425799434022 35.0 31.0 35.0 24.0 37.0 86-87 31.642225256471324 34.5 31.0 35.0 24.0 36.0 88-89 31.652563787610124 35.0 31.0 35.0 24.0 36.0 90-91 31.57298763073733 35.0 31.0 35.0 24.0 36.0 92-93 31.433812411691008 35.0 31.0 35.0 23.0 36.0 94-95 31.413438301785593 35.0 31.0 35.0 23.5 35.0 96-97 31.330558688203723 35.0 31.0 35.0 23.0 35.0 98-99 31.249261131435798 35.0 31.0 35.0 23.0 35.0 100 30.741884078564482 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8424856081380518 1101 2 0.9816969290816608 1101 3 1.0023391699085238 1101 4 1.038269032052746 1101 5 0.996064051322783 1101 6 0.7494106631826298 1101 7 0.8225257362308227 1101 8 0.8651234148881954 1101 9 1.0410542834549972 1101 10-11 1.042695074290421 1101 12-13 0.9412533535741403 1101 14-15 1.1765844775418302 1101 16-17 1.314644463642395 1101 18-19 1.4621471547421194 1101 20-21 1.075177289070048 1101 22-23 1.4116989130659618 1101 24-25 1.2457538206364163 1101 26-27 1.1888696912492307 1101 28-29 1.3634342924986171 1101 30-31 1.4319559614542499 1101 32-33 1.2347190412145679 1101 34-35 1.1033388725847573 1101 36-37 1.1338358445928733 1101 38-39 1.1586744593488731 1101 40-41 1.1113383407945676 1101 42-43 1.0931186943764288 1101 44-45 1.0875144830057621 1101 46-47 1.1340529868702376 1101 48-49 1.0911335550407983 1101 50-51 1.1108140558074169 1101 52-53 1.1287890411281083 1101 54-55 1.1697434113114724 1101 56-57 0.9868025710762751 1101 58-59 0.9476410823731882 1101 60-61 0.911107125386529 1101 62-63 0.8243749012430541 1101 64-65 0.8774830918730885 1101 66-67 0.9148539636968067 1101 68-69 0.9027333462530862 1101 70-71 0.8436790920815938 1101 72-73 0.8287511269215528 1101 74-75 0.7820761007422803 1101 76-77 0.818301802217519 1101 78-79 0.791620019254843 1101 80-81 0.7665894301479526 1101 82-83 0.7380538937882477 1101 84-85 0.8281111899378253 1101 86-87 0.7240138731860775 1101 88-89 0.7472199996007127 1101 90-91 0.8219085222976297 1101 92-93 0.847171439703839 1101 94-95 0.7933119310286081 1101 96-97 0.7539738153254056 1101 98-99 0.8544668648002052 1101 100 0.9611142641232675 1102 1 0.8738237982285213 1102 2 0.9680541856796872 1102 3 0.9493339684727751 1102 4 1.0828107144012193 1102 5 0.9569343048735064 1102 6 0.7245640451648896 1102 7 0.8290402690371579 1102 8 0.7856947978287039 1102 9 0.9538573383779294 1102 10-11 0.9815017220061861 1102 12-13 0.95121890069899 1102 14-15 1.237877326338392 1102 16-17 1.3991476704379977 1102 18-19 1.3358395971604509 1102 20-21 1.1234558174336797 1102 22-23 1.3780444987605662 1102 24-25 1.2083230346707055 1102 26-27 1.1453642123579115 1102 28-29 1.3398235399817153 1102 30-31 1.3320323473726958 1102 32-33 1.2092118605528768 1102 34-35 1.1083394955752937 1102 36-37 1.064914064586894 1102 38-39 1.1792502203500135 1102 40-41 1.1196269515428057 1102 42-43 1.1665332048749946 1102 44-45 1.2328132166008814 1102 46-47 1.1208791990578177 1102 48-49 1.0586494156090964 1102 50-51 1.1724708777250399 1102 52-53 1.126630919493799 1102 54-55 1.1984137942986521 1102 56-57 1.0505645818413853 1102 58-59 1.089198153016028 1102 60-61 1.1272864999896655 1102 62-63 1.0127349386053552 1102 64-65 0.9139264592503977 1102 66-67 1.032473185031428 1102 68-69 1.0690815598544958 1102 70-71 0.9965069124839161 1102 72-73 0.9436513210548227 1102 74-75 0.9410684499475295 1102 76-77 0.9164550848638342 1102 78-79 0.9274265768403609 1102 80-81 1.0060906748445007 1102 82-83 0.9744807551276864 1102 84-85 0.9326377888505242 1102 86-87 0.9077477508975385 1102 88-89 0.8962332874964112 1102 90-91 0.8816844640730572 1102 92-93 0.9017587908979543 1102 94-95 0.8931578050971787 1102 96-97 0.8737569921403896 1102 98-99 0.8823623096475579 1102 100 0.9476456797590025 1103 1 0.6798977718984567 1103 2 0.8411199779777228 1103 3 0.7212697693028041 1103 4 0.7508994514181424 1103 5 0.7169158628489924 1103 6 0.5028118593694089 1103 7 0.5765707043761594 1103 8 0.6335717655418094 1103 9 0.6461326019411899 1103 10-11 0.8081033236943398 1103 12-13 0.7631814496520093 1103 14-15 0.9112970978574566 1103 16-17 1.1384193471933983 1103 18-19 1.1801627355189765 1103 20-21 0.8682576021478141 1103 22-23 1.0941815701372377 1103 24-25 0.9947574762620306 1103 26-27 0.9107488819913527 1103 28-29 1.1286151004668596 1103 30-31 1.1078919285896944 1103 32-33 0.9284467094618662 1103 34-35 0.8787351014816664 1103 36-37 0.824626593058376 1103 38-39 0.9606127674440259 1103 40-41 0.8901661173100521 1103 42-43 0.9693907945738331 1103 44-45 1.005957130538242 1103 46-47 0.9921154340098326 1103 48-49 0.9249975359233389 1103 50-51 1.0336340498137773 1103 52-53 1.0264196381800303 1103 54-55 0.9864029678369874 1103 56-57 0.859656014638503 1103 58-59 0.8535340493688963 1103 60-61 0.9268297834633543 1103 62-63 0.8221538732458242 1103 64-65 0.8681877012331469 1103 66-67 0.8441824168951086 1103 68-69 0.8630687551687544 1103 70-71 0.8000897861626939 1103 72-73 0.7689805414012909 1103 74-75 0.7395173900850338 1103 76-77 0.7314795416493141 1103 78-79 0.7634612070563165 1103 80-81 0.7655890389159836 1103 82-83 0.6463978226523182 1103 84-85 0.6519312857320365 1103 86-87 0.7079462272013224 1103 88-89 0.7299042598600742 1103 90-91 0.7824136714280847 1103 92-93 0.8380115115910449 1103 94-95 0.7654663616864035 1103 96-97 0.7536453364523332 1103 98-99 0.8296743680922312 1103 100 0.9111560217973285 1104 1 0.5958999090451051 1104 2 0.7452656639851263 1104 3 0.7138624393636981 1104 4 0.738455688601583 1104 5 0.716327345461977 1104 6 0.49113385326516124 1104 7 0.4239051318648279 1104 8 0.4181236572426599 1104 9 0.5981606895251943 1104 10-11 0.5568098855124859 1104 12-13 0.49501850773712874 1104 14-15 0.7265531864031018 1104 16-17 0.8209642145343992 1104 18-19 0.7746472715582158 1104 20-21 0.6455822733481611 1104 22-23 0.9321627570818976 1104 24-25 0.7383550005101611 1104 26-27 0.595599669125825 1104 28-29 0.8991144456616524 1104 30-31 0.9226832553641771 1104 32-33 0.7825957295186896 1104 34-35 0.6455242027257384 1104 36-37 0.6045185085232418 1104 38-39 0.7535323237512443 1104 40-41 0.7108169048848652 1104 42-43 0.6238237278107519 1104 44-45 0.6883657920002335 1104 46-47 0.6358467369909491 1104 48-49 0.6660040293515408 1104 50-51 0.7292824783913687 1104 52-53 0.7187129829455827 1104 54-55 0.656428400972473 1104 56-57 0.6420725813623207 1104 58-59 0.5762952456677581 1104 60-61 0.6342594373795905 1104 62-63 0.5135573256225499 1104 64-65 0.5641571631228075 1104 66-67 0.6694585427632376 1104 68-69 0.5866643418938224 1104 70-71 0.530803450449703 1104 72-73 0.5150140055069485 1104 74-75 0.5341396181715581 1104 76-77 0.4573811792232547 1104 78-79 0.5069384879733576 1104 80-81 0.5620051926580203 1104 82-83 0.47658286449201626 1104 84-85 0.41765104042568524 1104 86-87 0.48693957440974955 1104 88-89 0.45183242870891505 1104 90-91 0.5060085897706053 1104 92-93 0.4622662912601747 1104 94-95 0.4604409558104443 1104 96-97 0.567835774209879 1104 98-99 0.5712125926018743 1104 100 0.6888693640025885 1105 1 0.5011385883440944 1105 2 0.6819316817584351 1105 3 0.662536069492095 1105 4 0.7548488049179056 1105 5 0.6971103487554089 1105 6 0.3605374963833654 1105 7 0.43215194940118806 1105 8 0.4907553258659547 1105 9 0.5886540037340922 1105 10-11 0.6088193848335379 1105 12-13 0.5751375299065913 1105 14-15 0.7092730169424115 1105 16-17 0.8628200754148452 1105 18-19 0.8782635482718248 1105 20-21 0.6762714956275744 1105 22-23 0.9132736406158912 1105 24-25 0.7133762885913413 1105 26-27 0.5804066074280101 1105 28-29 0.9065493503121473 1105 30-31 0.8339612707377739 1105 32-33 0.743430885127701 1105 34-35 0.6459683264390534 1105 36-37 0.5989653946114046 1105 38-39 0.7262117736170666 1105 40-41 0.620668001773403 1105 42-43 0.6108730803306273 1105 44-45 0.6421211926441046 1105 46-47 0.6583307425658589 1105 48-49 0.662576638328801 1105 50-51 0.7255375078125113 1105 52-53 0.7478704884721239 1105 54-55 0.6524384532462051 1105 56-57 0.6546485725469253 1105 58-59 0.6352155587767143 1105 60-61 0.693263250722822 1105 62-63 0.6272270205909152 1105 64-65 0.5918188798876329 1105 66-67 0.6758954753049977 1105 68-69 0.5779593678276598 1105 70-71 0.600404279520454 1105 72-73 0.5560425273557428 1105 74-75 0.5935049507055155 1105 76-77 0.5956809054539534 1105 78-79 0.6218722809461212 1105 80-81 0.6262670326727644 1105 82-83 0.6297136356122053 1105 84-85 0.5152092208809371 1105 86-87 0.5525363234142198 1105 88-89 0.5983651884682892 1105 90-91 0.6342456918208335 1105 92-93 0.6518928849800822 1105 94-95 0.5784268366075445 1105 96-97 0.6420817641086352 1105 98-99 0.7246507918790108 1105 100 0.7532414760788129 1106 1 0.5163575571471597 1106 2 0.5803437595678602 1106 3 0.5923714225562797 1106 4 0.7243047355942238 1106 5 0.5930723114673313 1106 6 0.28699830013294303 1106 7 0.35278808873865586 1106 8 0.3960065595098925 1106 9 0.5922783569596177 1106 10-11 0.48054138243414 1106 12-13 0.41735600828727115 1106 14-15 0.6259713001445633 1106 16-17 0.8407066384804551 1106 18-19 0.8119499950418216 1106 20-21 0.5933565655407236 1106 22-23 0.8383894270249641 1106 24-25 0.7938857827374264 1106 26-27 0.6165219928728405 1106 28-29 0.8661407721536776 1106 30-31 0.8218969000040133 1106 32-33 0.7062917729040379 1106 34-35 0.6871356515283935 1106 36-37 0.6022447448736301 1106 38-39 0.7267745313084717 1106 40-41 0.6579355104117042 1106 42-43 0.6782024229250112 1106 44-45 0.6411548748376177 1106 46-47 0.6041899382252112 1106 48-49 0.7313799937284813 1106 50-51 0.6941507746166664 1106 52-53 0.6483074556170223 1106 54-55 0.7161873230382625 1106 56-57 0.6536580431303634 1106 58-59 0.6489923143530731 1106 60-61 0.6296723304859526 1106 62-63 0.5994713300138912 1106 64-65 0.6167755086570494 1106 66-67 0.6230900036608347 1106 68-69 0.5820399476602773 1106 70-71 0.5724129126317834 1106 72-73 0.5055719035768078 1106 74-75 0.5521254620227651 1106 76-77 0.5119694882770247 1106 78-79 0.5330450254424477 1106 80-81 0.5500233746169769 1106 82-83 0.6244596663059383 1106 84-85 0.5586091559439019 1106 86-87 0.5474394550087602 1106 88-89 0.5309515794866932 1106 90-91 0.5311761688564438 1106 92-93 0.5930739982069504 1106 94-95 0.5497931381997354 1106 96-97 0.5541646155394098 1106 98-99 0.5095862981402881 1106 100 0.5471744160092591 1107 1 0.4854014288764539 1107 2 0.6366835195521219 1107 3 0.5598422403148184 1107 4 0.7175981482770268 1107 5 0.5873289020634402 1107 6 0.31957461661271225 1107 7 0.35168801660277893 1107 8 0.40301849471875784 1107 9 0.5014555980901818 1107 10-11 0.4728123920085423 1107 12-13 0.40211115616581594 1107 14-15 0.5547531055088513 1107 16-17 0.7081141324144795 1107 18-19 0.824704724860176 1107 20-21 0.6045400857715606 1107 22-23 0.8707905270970997 1107 24-25 0.5887590121295148 1107 26-27 0.5134365446467299 1107 28-29 0.6644013138285416 1107 30-31 0.677982135101395 1107 32-33 0.6451047688381948 1107 34-35 0.5393187864880602 1107 36-37 0.5749665217114526 1107 38-39 0.5934018511327181 1107 40-41 0.4264760704484232 1107 42-43 0.5591999473528375 1107 44-45 0.5373509368744749 1107 46-47 0.5008337755260257 1107 48-49 0.4403607055784349 1107 50-51 0.5163960857846135 1107 52-53 0.5534209056793244 1107 54-55 0.5111132198183839 1107 56-57 0.4555600121119454 1107 58-59 0.5517963916696189 1107 60-61 0.5859333967853786 1107 62-63 0.45928816226518876 1107 64-65 0.48935342360228873 1107 66-67 0.4713741124548605 1107 68-69 0.5211556273769844 1107 70-71 0.4684085516900893 1107 72-73 0.4245131046391748 1107 74-75 0.3818054820240775 1107 76-77 0.3712606713054214 1107 78-79 0.3815186958470633 1107 80-81 0.4910877476906208 1107 82-83 0.4739243853039028 1107 84-85 0.4971063524813708 1107 86-87 0.5921442307317584 1107 88-89 0.5641793731125055 1107 90-91 0.5242148517208953 1107 92-93 0.5899264557451538 1107 94-95 0.5590750993119435 1107 96-97 0.5229402319732728 1107 98-99 0.5276450642888868 1107 100 0.6544785834956492 1108 1 0.3177177436349652 1108 2 0.4542480827814259 1108 3 0.46433873325061725 1108 4 0.591299347809148 1108 5 0.38896003241566746 1108 6 0.26225557291360957 1108 7 0.2092006147945682 1108 8 0.22889986742094237 1108 9 0.2942236802007514 1108 10-11 0.3944287685562813 1108 12-13 0.2467531942688339 1108 14-15 0.41314539898062463 1108 16-17 0.5462135855247681 1108 18-19 0.5823473983807403 1108 20-21 0.34885522683525494 1108 22-23 0.5835706941583325 1108 24-25 0.381203214057102 1108 26-27 0.36240362811525983 1108 28-29 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-3.891431737870672 2215 94-95 -2.0557275497024037 2215 96-97 -1.6532170620653055 2215 98-99 -3.063807322039576 2215 100 -3.0766390134903503 2216 1 -0.7208743408769713 2216 2 -0.6441443646277172 2216 3 -0.9294518668563825 2216 4 -0.9106735457418793 2216 5 -1.0446497649361746 2216 6 -0.8466965024866155 2216 7 -0.9895392818172581 2216 8 -0.8947217035801032 2216 9 -1.1245665053411358 2216 10-11 -1.1055175561712645 2216 12-13 -1.2825351763506276 2216 14-15 -1.649410294569357 2216 16-17 -1.3831161543290733 2216 18-19 -3.300563704022089 2216 20-21 -1.611621880879241 2216 22-23 -1.1131819082196088 2216 24-25 -1.277789488724153 2216 26-27 -1.228908483557234 2216 28-29 -1.2067680425712624 2216 30-31 -1.3120272581552044 2216 32-33 -1.32067264775975 2216 34-35 -1.5222016362230022 2216 36-37 -1.5147404459459324 2216 38-39 -1.626637749598217 2216 40-41 -1.690371202264764 2216 42-43 -1.6845939943112143 2216 44-45 -1.4889709909837663 2216 46-47 -1.724551935980493 2216 48-49 -1.6472017346550913 2216 50-51 -1.5617523489629832 2216 52-53 -1.5330794698573627 2216 54-55 -1.4936627986797077 2216 56-57 -1.5531606297267189 2216 58-59 -1.5423867942558829 2216 60-61 -1.540707809848847 2216 62-63 -1.5402768128409932 2216 64-65 -1.459669563985642 2216 66-67 -1.5227556466015812 2216 68-69 -1.5991330083795319 2216 70-71 -1.752196338223463 2216 72-73 -1.5997133309175702 2216 74-75 -1.614076789643807 2216 76-77 -1.6106812405247624 2216 78-79 -1.6978886820938293 2216 80-81 -1.654686532982403 2216 82-83 -1.7353580492578615 2216 84-85 -1.598307647681228 2216 86-87 -1.5545301281458102 2216 88-89 -1.483947310995326 2216 90-91 -1.50465565635702 2216 92-93 -1.4613941049853274 2216 94-95 -1.3489964351038672 2216 96-97 -1.3517377670936916 2216 98-99 -1.3734021434796908 2216 100 -1.387297574376877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16211.0 1 2766.0 2 457.0 3 426.0 4 407.0 5 238.0 6 186.0 7 240.0 8 457.0 9 763.0 10 1360.0 11 2473.0 12 4088.0 13 6135.0 14 8088.0 15 10492.0 16 13159.0 17 15330.0 18 17737.0 19 20715.0 20 23701.0 21 26901.0 22 31087.0 23 36255.0 24 41002.0 25 46559.0 26 53115.0 27 61226.0 28 72552.0 29 86858.0 30 102827.0 31 124208.0 32 153075.0 33 195110.0 34 267357.0 35 361235.0 36 528420.0 37 796859.0 38 647558.0 39 219502.0 40 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21061317882458 21.472502383879668 12.543199133667557 23.7736853036282 2 15.277895039430067 20.032520006513046 41.06363186809873 23.62595308595815 3 18.5388516485671 27.077839031320412 28.884762681343386 25.498546638769103 4 12.043828549125537 16.897133144624007 40.60340908262689 30.455629223623564 5 12.965995982679546 38.81691294423864 35.162231660454765 13.054859412627048 6 29.365883578784103 38.92666139608426 18.391248989770126 13.316206035361503 7 26.65823891714481 31.754865127093833 24.10220707976216 17.484688875999193 8 25.850236657007624 35.38322092387376 20.114073077672526 18.65246934144609 9 25.893139146336313 15.105309798604253 21.90782286991456 37.093728185144876 10-11 25.612884809571057 25.938626638046053 27.68956441587413 20.758924136508764 12-13 25.8249865574699 23.706260220221086 27.647185015992715 22.821568206316304 14-15 23.23567338711452 24.92596441790384 25.044919299926626 26.79344289505501 16-17 22.150305848930092 27.466006026941663 26.531973423511108 23.85171470061713 18-19 21.492672669568652 28.286522191108265 28.052268428731793 22.16853671059129 20-21 23.391564684155057 27.451739301502943 26.80801183111563 22.348684183226368 22-23 23.86663854385512 27.012643449593003 26.579614162735044 22.54110384381683 24-25 23.273818086344555 27.13725005960243 27.229118276368165 22.35981357768485 26-27 23.153075921882714 27.339428365395673 26.177774240927633 23.329721471793977 28-29 22.15614868218637 27.9159163040368 26.969277584973067 22.958657428803768 30-31 23.057626621696024 27.44547051642941 26.891597839628755 22.60530502224581 32-33 23.46619750256367 27.637438712023332 25.862872348504467 23.033491436908527 34-35 23.46576852474304 27.46401157484428 26.83138637833172 22.238833522080956 36-37 23.107111787419235 27.07146298753706 26.41091805809349 23.410507166950214 38-39 23.267355619675403 27.12049484291308 27.10010945982382 22.5120400775877 40-41 22.894406413367623 26.184559885866527 27.82500221344892 23.096031487316935 42-43 22.840218588636162 26.431288865815837 27.098755627357008 23.629736918190993 44-45 23.110796955259623 27.36261690706297 26.86313006555348 22.66345607212392 46-47 22.907768174117056 27.14813032140912 26.384718738566715 23.55938276590711 48-49 23.657793920483385 26.669865366666706 26.790985952753037 22.881354760096873 50-51 23.249673985078136 26.542556736656653 26.77957618688923 23.428193091375977 52-53 22.697807988256493 27.149569910604633 26.55435943419608 23.5982626669428 54-55 22.711102017440815 27.04424289975851 26.52984409707127 23.714810985729407 56-57 23.846422157747913 26.433703271248376 26.239232498070507 23.480642072933204 58-59 22.61534509185193 26.870239105862005 27.276216205408694 23.23819959687738 60-61 22.684899006994744 27.550828460014475 26.48828819132012 23.27598434167066 62-63 23.21124922358882 26.76808000744349 26.003764494056526 24.016906274911168 64-65 22.2563086124429 26.928705162742606 26.61488570098146 24.20010052383304 66-67 23.14555160111024 26.501962335679302 26.49016732195219 23.862318741258267 68-69 22.39151468852021 26.698880336224885 26.791964500987252 24.117640474267652 70-71 22.333754313813344 26.22501735711364 27.02436887195801 24.41685945711501 72-73 23.014085374940343 26.414935364219588 27.292251566142028 23.278727694698038 74-75 23.18493864293512 26.722311793712798 26.798065758817934 23.29468380453415 76-77 22.777989227506033 26.669196519114152 26.420335487102847 24.132478766276964 78-79 22.742884885317984 26.658348719857923 26.489897719440346 24.10886867538374 80-81 22.271478921101835 27.005952341283155 27.176184498562684 23.546384239052323 82-83 23.170920628150483 25.930370699723266 28.240290395365424 22.658418276760823 84-85 23.382961262197284 26.468547306618458 26.785399449243673 23.363091981940585 86-87 21.876078497460043 27.193083589481738 27.369767962848222 23.561069950209998 88-89 23.069833586096497 25.762011056317302 27.217590444249755 23.950564913336446 90-91 22.681535561601983 25.92911518229593 27.25779292264714 24.131556333454952 92-93 22.88799095824791 26.180751321845324 27.289306853386552 23.64195086652021 94-95 21.767409825151777 26.10534095732222 27.97029001746813 24.156959200057873 96-97 22.14190334781788 25.97823157709969 27.547992673799044 24.331872401283388 98-99 21.48729447880845 26.738247269602173 27.350855899386712 24.423602352202668 100 22.255163986084373 25.855564176670587 26.529115483713678 25.36015635353136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13591.0 1 8006.0 2 2188.0 3 1540.0 4 815.5 5 416.0 6 306.0 7 292.0 8 283.0 9 265.0 10 266.5 11 244.5 12 217.0 13 221.5 14 248.0 15 309.5 16 343.5 17 397.5 18 514.0 19 610.5 20 739.0 21 946.5 22 1401.0 23 1992.5 24 2632.5 25 3609.0 26 5467.5 27 7725.0 28 11002.0 29 17878.0 30 28117.5 31 38519.5 32 51194.5 33 64989.5 34 78001.5 35 90587.0 36 102870.5 37 123858.5 38 133383.0 39 125856.0 40 117476.0 41 109352.5 42 108205.5 43 114918.0 44 126121.5 45 148777.5 46 170770.0 47 185712.0 48 194074.0 49 189290.0 50 180788.0 51 184458.0 52 186333.5 53 176547.5 54 165294.0 55 142786.5 56 124358.5 57 101892.5 58 78813.5 59 74168.0 60 60961.5 61 41738.0 62 30607.0 63 25275.0 64 18617.0 65 8782.5 66 4539.0 67 2979.0 68 2095.5 69 1567.5 70 1193.0 71 863.0 72 636.0 73 558.5 74 462.5 75 363.0 76 259.5 77 161.5 78 98.5 79 72.0 80 66.0 81 51.0 82 41.0 83 29.5 84 19.0 85 18.0 86 13.0 87 8.5 88 7.5 89 6.5 90 6.5 91 5.0 92 4.0 93 6.0 94 4.5 95 1.0 96 2.5 97 3.0 98 2.0 99 3.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.98800999067377 2 0.4408945175377152 3 0.4744169104962115 4 0.4595319673541926 5 0.42686014257524024 6 0.4821470742455961 7 0.500109072860671 8 0.5016601089527805 9 0.5173455868520171 10-11 0.5296788254554042 12-13 0.5289033074093494 14-15 0.5261139441146686 16-17 0.5299039758558717 18-19 0.5274648465174737 20-21 0.5261889942481578 22-23 0.4887514859926431 24-25 0.5248506002009342 26-27 0.5041993051358307 28-29 0.5093902727021651 30-31 0.513130271021042 32-33 0.5155944170706032 34-35 0.5283279230525991 36-37 0.5459271793558097 38-39 0.5363582873359405 40-41 0.5411614958792473 42-43 0.5390976172082952 44-45 0.5490667766067733 46-47 0.5458271125111575 48-49 0.5181586299648165 50-51 0.5324306636833405 52-53 0.525438492913266 54-55 0.535970528312913 56-57 0.5227241797520743 58-59 0.5283154146970176 60-61 0.5231994972641725 62-63 0.5177958876529521 64-65 0.5292660497212138 66-67 0.5271396292723539 68-69 0.5477033658483867 70-71 0.5326683224393896 72-73 0.5352450436891844 74-75 0.5328184227063678 76-77 0.5206352843699591 78-79 0.5500674450532956 80-81 0.5251883258016354 82-83 0.5242501991330208 84-85 0.5338816329307978 86-87 0.8375594897391458 88-89 1.1012981671756734 90-91 1.0922421177346466 92-93 1.0924672681351142 94-95 1.0967826508107414 96-97 1.090065663863461 98-99 1.0861505485664424 100 1.0810221227780157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3997328.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.335230417496703 #Duplication Level Percentage of deduplicated Percentage of total 1 74.25736332053538 21.783568632036737 2 12.729613033431692 7.468522629225757 3 4.938255422374901 4.345945820274608 4 2.524967956430939 2.962820671947896 5 1.4123305309770353 2.0715520775938403 6 0.8384877735918568 1.4758339224342558 7 0.5854622758289915 1.2022269533536833 8 0.39801693418611217 0.9340734779532178 9 0.2943419617081688 0.7771130341422412 >10 1.5125725664870817 8.367354372644481 >50 0.1935193295950031 4.036039908923169 >100 0.2358995245816157 15.422154552393152 >500 0.04635967430299702 9.6170250731553 >1k 0.031531396125189154 16.327335573545447 >5k 9.374198848029209E-4 1.9082603294710838 >10k+ 3.408799581101531E-4 1.300172970905153 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 15282 0.3823053799938359 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 13443 0.3362996481649742 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12076 0.30210180400507536 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11207 0.28036228200437896 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 9623 0.24073581152209678 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8647 0.2163195014269532 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8537 0.21356766319901693 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7142 0.17866935112655252 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 6669 0.16683644674642661 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 6483 0.16218333847009803 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6262 0.15665464530306245 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6102 0.15265197151697335 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5839 0.14607257648108937 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5575 0.13946816473404236 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5453 0.1364161259721494 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4977 0.12450817145853431 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4892 0.12238175100967447 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 4873 0.12190643349757639 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 4853 0.12140609927431523 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 4755 0.11895446158033567 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4695 0.11745345891055224 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4688 0.11727834193241085 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 4663 0.11665292415333442 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4607 0.11525198832820324 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4587 0.1147516541049421 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4569 0.11430135330400709 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 4484 0.11217493285514722 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4408 0.11027366280675491 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4310 0.10782202511277533 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4207 0.10524530386298048 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4173 0.10439473568343653 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4032 0.10086737940944553 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5016711163056923E-5 2 0.0 0.0 0.0 0.0 2.5016711163056923E-5 3 0.0 0.0 0.0 0.0 2.5016711163056923E-5 4 0.0 0.0 0.0 0.0 2.5016711163056923E-5 5 0.0 0.0 0.0 0.0 2.5016711163056923E-5 6 0.0 0.0 0.0 0.0 2.5016711163056923E-5 7 0.0 0.0 0.0 0.0 2.5016711163056923E-5 8 0.0 0.0 0.0 0.0 2.5016711163056923E-5 9 0.0 0.0 0.0 0.0 2.5016711163056923E-5 10-11 0.0 0.0 0.0 0.0 2.5016711163056923E-5 12-13 0.0 0.0 0.0 0.0 3.752506674458538E-5 14-15 0.0 0.0 0.0 0.0 6.25417779076423E-5 16-17 0.0 0.0 0.0 0.0 7.505013348917076E-5 18-19 0.0 0.0 0.0 2.5016711163056923E-5 7.505013348917076E-5 20-21 0.0 0.0 0.0 7.505013348917076E-5 1.1257520023375616E-4 22-23 0.0 0.0 0.0 1.0006684465222769E-4 1.5010026697834152E-4 24-25 0.0 0.0 0.0 1.7511697814139845E-4 1.5010026697834152E-4 26-27 0.0 0.0 0.0 3.127088895382115E-4 1.7511697814139845E-4 28-29 0.0 0.0 0.0 0.0011507687135006183 1.7511697814139845E-4 30-31 0.0 0.0 0.0 0.005103409077263612 1.7511697814139845E-4 32-33 0.0 0.0 0.0 0.014422133985502315 1.7511697814139845E-4 34-35 0.0 0.0 0.0 0.029794902995200795 1.7511697814139845E-4 36-37 0.0 0.0 0.0 0.05753843567503092 1.7511697814139845E-4 38-39 0.0 0.0 0.0 0.10304383328063146 1.7511697814139845E-4 40-41 0.0 0.0 0.0 0.15490347552164846 1.7511697814139845E-4 42-43 0.0 0.0 0.0 0.21224177750737494 1.7511697814139845E-4 44-45 0.0 0.0 0.0 0.28161311756253177 1.7511697814139845E-4 46-47 0.0 0.0 0.0 0.3673328783627463 1.7511697814139845E-4 48-49 0.0 0.0 0.0 0.475517645787386 1.8762533372292692E-4 50-51 0.0 0.0 0.0 0.5909822761604753 2.0013368930445538E-4 52-53 0.0 0.0 0.0 0.7086984105382395 2.0013368930445538E-4 54-55 0.0 0.0 0.0 0.8376470482282165 2.0013368930445538E-4 56-57 0.0 0.0 0.0 0.9981667754059712 2.1264204488598385E-4 58-59 0.0 0.0 0.0 1.1918586615859394 2.2515040046751228E-4 60-61 0.0 0.0 0.0 1.3972333518790552 2.2515040046751228E-4 62-63 0.0 0.0 0.0 1.6140907125960142 2.2515040046751228E-4 64-65 0.0 0.0 0.0 1.8438191712063658 2.5016711163056924E-4 66-67 0.0 0.0 0.0 2.1085585170894157 2.5016711163056924E-4 68-69 0.0 0.0 0.0 2.4080460747779515 2.5016711163056924E-4 70-71 0.0 0.0 0.0 2.7418815768933644 2.5016711163056924E-4 72-73 0.0 0.0 0.0 3.0950049633154944 2.6267546721209765E-4 74-75 0.0 0.0 0.0 3.4399854102540495 2.751838227936261E-4 76-77 0.0 0.0 0.0 3.8350993463633705 2.751838227936261E-4 78-79 0.0 0.0 0.0 4.273317075806639 3.0020053395668305E-4 80-81 0.0 0.0 0.0 4.740591715265797 3.0020053395668305E-4 82-83 0.0 0.0 0.0 5.23801149167644 3.0020053395668305E-4 84-85 0.0 0.0 0.0 5.717018968671073 3.0020053395668305E-4 86-87 0.0 0.0 0.0 6.236265825571482 3.0020053395668305E-4 88 0.0 0.0 0.0 6.661825099166243 3.0020053395668305E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 3985 0.0 38.974174 94 GTACTAG 4715 0.0 35.411682 1 GCATCGT 4480 0.0 34.685574 8 TAGGCAT 5655 0.0 31.814264 5 TATTCTC 2230 0.0 30.543505 5 CAACGCA 7515 0.0 29.190641 5 CTAGGCA 6465 0.0 28.484694 4 GTATCAA 8610 0.0 27.828226 1 TACCCCG 220 3.6379788E-12 27.75725 5 ATCAACG 7950 0.0 27.719616 3 CTGTGCG 6440 0.0 26.02607 9 CATCGTC 6065 0.0 25.854841 9 TAGTACT 6710 0.0 25.83437 4 GTACATG 16830 0.0 25.67062 1 AGGGCTA 4785 0.0 25.622076 5 CGTTAAC 940 0.0 25.589823 1 CTATTGA 4685 0.0 25.553877 9 GGTATCA 5910 0.0 25.378422 1 TACATGG 16615 0.0 25.339937 2 GCTATTG 4735 0.0 25.084156 8 >>END_MODULE