##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3997328
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84537245880248	31.0	31.0	34.0	30.0	34.0
2	31.79901123950799	31.0	31.0	34.0	30.0	34.0
3	31.984560686538607	33.0	31.0	34.0	30.0	34.0
4	35.727246300528755	37.0	35.0	37.0	35.0	37.0
5	35.63978462613026	37.0	35.0	37.0	33.0	37.0
6	35.676112142911464	37.0	35.0	37.0	33.0	37.0
7	35.59325604503808	37.0	35.0	37.0	33.0	37.0
8	35.575448399530885	37.0	35.0	37.0	33.0	37.0
9	37.1604992134746	39.0	37.0	39.0	33.0	39.0
10-11	37.11211927067281	39.0	37.0	39.0	33.0	39.0
12-13	37.047136987507656	39.0	37.0	39.0	33.0	39.0
14-15	38.23976353704275	40.0	37.0	41.0	33.0	41.0
16-17	38.2369168854795	40.0	37.0	41.0	33.0	41.0
18-19	38.286795829614185	40.0	37.5	41.0	33.0	41.0
20-21	38.30168202359176	40.0	38.0	41.0	33.0	41.0
22-23	38.26498988824535	40.0	37.5	41.0	33.0	41.0
24-25	38.192957145373114	40.0	37.0	41.0	33.0	41.0
26-27	38.049178100971446	40.0	37.0	41.0	33.0	41.0
28-29	37.987373190291116	40.0	37.0	41.0	33.0	41.0
30-31	37.87216247953633	40.0	37.0	41.0	33.0	41.0
32-33	37.75757681130996	40.0	36.5	41.0	32.5	41.0
34-35	37.67156347940424	40.0	36.0	41.0	32.0	41.0
36-37	37.59556708881533	40.0	36.0	41.0	32.0	41.0
38-39	37.48124046863305	40.0	36.0	41.0	32.0	41.0
40-41	37.35955330660882	40.0	36.0	41.0	31.0	41.0
42-43	37.2402573669211	39.0	36.0	41.0	31.0	41.0
44-45	37.04527937162024	39.0	35.0	41.0	31.0	41.0
46-47	36.809775680154345	39.0	35.0	41.0	30.5	41.0
48-49	36.56417199189058	39.0	35.0	41.0	30.0	41.0
50-51	36.358971542990716	39.0	35.0	41.0	30.0	41.0
52-53	36.16457468589017	38.0	35.0	40.5	29.0	41.0
54-55	35.96117506494338	38.0	35.0	40.0	29.0	41.0
56-57	35.6998711389208	37.5	34.0	40.0	29.0	41.0
58-59	35.418543211865526	37.0	34.0	40.0	28.0	41.0
60-61	35.16252494166103	36.5	34.0	40.0	28.0	41.0
62-63	34.88673796596126	36.0	34.0	39.5	28.0	41.0
64-65	34.61312006420289	35.5	33.5	39.0	27.0	41.0
66-67	34.39484313021098	35.0	33.0	39.0	27.0	41.0
68-69	34.20226874051866	35.0	33.0	38.5	27.0	41.0
70-71	34.004695761768865	35.0	33.0	38.0	27.0	40.0
72-73	33.80801312776935	35.0	33.0	37.0	27.0	40.0
74-75	33.62849883722326	35.0	33.0	37.0	27.0	39.5
76-77	33.467783604447774	35.0	33.0	36.0	27.0	39.0
78-79	33.34807176193698	35.0	33.0	36.0	27.0	39.0
80-81	33.201572525447	35.0	33.0	36.0	27.0	38.0
82-83	33.01270361101216	35.0	33.0	35.0	27.0	37.0
84-85	32.87133267522705	35.0	33.0	35.0	27.0	37.0
86-87	32.74201579154875	35.0	33.0	35.0	27.0	36.5
88-89	32.63719527144132	35.0	33.0	35.0	27.0	36.0
90-91	32.54039836110522	35.0	33.0	35.0	27.0	36.0
92-93	32.46170404330093	35.0	33.0	35.0	27.0	36.0
94-95	32.42294652828089	35.0	33.0	35.0	27.0	35.0
96-97	32.37507992839217	35.0	33.0	35.0	26.5	35.0
98-99	32.33306873991826	35.0	33.0	35.0	26.0	35.0
100	31.831273540725203	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46527531966787805
1101	2	0.5665487716379332
1101	3	0.4989531720608227
1101	4	0.38209491874258816
1101	5	0.3712311894040141
1101	6	0.40001607599039346
1101	7	0.3964487679207096
1101	8	0.4043186203072935
1101	9	0.4708444953315194
1101	10-11	0.5588038253336904
1101	12-13	0.5832318298700443
1101	14-15	0.708854870448441
1101	16-17	0.6775718963321893
1101	18-19	0.6458815855888957
1101	20-21	0.6567917594869925
1101	22-23	0.6394084413451822
1101	24-25	0.6915297330563632
1101	26-27	0.7080671729955554
1101	28-29	0.7376187172213875
1101	30-31	0.7464906798725224
1101	32-33	0.7644820845278346
1101	34-35	0.7492197253928552
1101	36-37	0.7738070982520782
1101	38-39	0.825697533376335
1101	40-41	0.8803740885891003
1101	42-43	0.8434105232396405
1101	44-45	0.8097854810227858
1101	46-47	0.8128451736898867
1101	48-49	0.834599954885455
1101	50-51	0.8034697258518264
1101	52-53	0.7919668753294928
1101	54-55	0.7339885131519992
1101	56-57	0.8253039462864322
1101	58-59	0.7798934642526234
1101	60-61	0.7375581941446967
1101	62-63	0.6866100708988725
1101	64-65	0.6833895801646648
1101	66-67	0.6616424958118117
1101	68-69	0.6860019636179473
1101	70-71	0.6412916313055064
1101	72-73	0.6185256567061117
1101	74-75	0.5793705052888427
1101	76-77	0.6090267442988946
1101	78-79	0.5839553781671043
1101	80-81	0.5781027254709628
1101	82-83	0.6029149949328314
1101	84-85	0.6175309790460162
1101	86-87	0.6607194763338597
1101	88-89	0.6144231288014126
1101	90-91	0.6180388294263963
1101	92-93	0.615096843455575
1101	94-95	0.5874165888989182
1101	96-97	0.5975562172634596
1101	98-99	0.6163435596328029
1101	100	0.6019864317251056
1102	1	0.4720976547562934
1102	2	0.5710296471130434
1102	3	0.4551041117489163
1102	4	0.30083185146261116
1102	5	0.32546354303656244
1102	6	0.363556197223069
1102	7	0.41640790744875034
1102	8	0.4151807064735209
1102	9	0.5039906424883114
1102	10-11	0.5540291969764439
1102	12-13	0.567636808613436
1102	14-15	0.7337022912890419
1102	16-17	0.7235692570788146
1102	18-19	0.7003144469052032
1102	20-21	0.695177877887744
1102	22-23	0.6793782883892376
1102	24-25	0.6725374866124412
1102	26-27	0.6991291856018051
1102	28-29	0.7403679573227961
1102	30-31	0.7677152823039464
1102	32-33	0.7841305851330631
1102	34-35	0.7428630810810617
1102	36-37	0.7570480641400366
1102	38-39	0.7132641997860958
1102	40-41	0.7454008878863831
1102	42-43	0.7853134713764263
1102	44-45	0.7359546533531045
1102	46-47	0.7191687791651802
1102	48-49	0.733207282024857
1102	50-51	0.7175943609955979
1102	52-53	0.6927051619299789
1102	54-55	0.728361474249553
1102	56-57	0.8146922605682434
1102	58-59	0.790780999903312
1102	60-61	0.7530028199200629
1102	62-63	0.6973553463398972
1102	64-65	0.766479283522699
1102	66-67	0.7754585579635744
1102	68-69	0.6978819488802586
1102	70-71	0.6803303108824252
1102	72-73	0.7109612349890497
1102	74-75	0.6840226656958421
1102	76-77	0.6416015491915275
1102	78-79	0.5498019024151333
1102	80-81	0.5409428654575805
1102	82-83	0.5541889731106124
1102	84-85	0.5470389042991428
1102	86-87	0.5402970116805434
1102	88-89	0.537045108635354
1102	90-91	0.6092108020028206
1102	92-93	0.5743962135286438
1102	94-95	0.550879318008505
1102	96-97	0.5268988948125468
1102	98-99	0.5087333181394058
1102	100	0.5422609607464004
1103	1	0.3037899707127103
1103	2	-0.13297912892579333
1103	3	0.2586793386291575
1103	4	0.24559535084513584
1103	5	0.2607578469660581
1103	6	0.2548866245207648
1103	7	0.3187095451714299
1103	8	0.3246955719579603
1103	9	0.4092417017455361
1103	10-11	0.4544346583361687
1103	12-13	0.42008622409539953
1103	14-15	0.5865123627549949
1103	16-17	0.5422252406365189
1103	18-19	0.5220515093326981
1103	20-21	0.523756676876701
1103	22-23	0.519651406633713
1103	24-25	0.4755176817825202
1103	26-27	0.47502735207784497
1103	28-29	0.5360658468496311
1103	30-31	0.5769453842533281
1103	32-33	0.5484632795579145
1103	34-35	0.5008309911153646
1103	36-37	0.5528490098139329
1103	38-39	0.5281645379529252
1103	40-41	0.5812959707841685
1103	42-43	0.6002916015326036
1103	44-45	0.6378281108884565
1103	46-47	0.6532340155977039
1103	48-49	0.617997102606644
1103	50-51	0.6739525550887464
1103	52-53	0.6454427320184237
1103	54-55	0.6217983918904508
1103	56-57	0.7096693596225521
1103	58-59	0.612768001858953
1103	60-61	0.6533554832432529
1103	62-63	0.6398971805426115
1103	64-65	0.636592925216739
1103	66-67	0.6951901812746044
1103	68-69	0.5795229232045216
1103	70-71	0.5402664014801815
1103	72-73	0.5308438677099119
1103	74-75	0.5452422565667234
1103	76-77	0.55883957311611
1103	78-79	0.49987360398615266
1103	80-81	0.4793610757327542
1103	82-83	0.458124742488792
1103	84-85	0.454301206673982
1103	86-87	0.5430100309522032
1103	88-89	0.4990519630850514
1103	90-91	0.4673809685073991
1103	92-93	0.4772260072499108
1103	94-95	0.5118403579212298
1103	96-97	0.46193178901609144
1103	98-99	0.4916347658756166
1103	100	0.5414899310776882
1104	1	0.3284246380537361
1104	2	0.3856291392441733
1104	3	0.35023272886989076
1104	4	0.2400384445452417
1104	5	0.21969968363517012
1104	6	0.26185020086960975
1104	7	0.2419473296809116
1104	8	0.2730894408297928
1104	9	0.28445973861740015
1104	10-11	0.3109305792105843
1104	12-13	0.3402269335622492
1104	14-15	0.3912213091523924
1104	16-17	0.4225259860481927
1104	18-19	0.4443154866796135
1104	20-21	0.38589670831531464
1104	22-23	0.36771810617955225
1104	24-25	0.3919001023883695
1104	26-27	0.4738836053758604
1104	28-29	0.4012555636976245
1104	30-31	0.4116190342857138
1104	32-33	0.45137000711396524
1104	34-35	0.45613335375069397
1104	36-37	0.38781602736308685
1104	38-39	0.39814874630123853
1104	40-41	0.37774159888604686
1104	42-43	0.34812973783672874
1104	44-45	0.36852520977269876
1104	46-47	0.3866618110434885
1104	48-49	0.3847224716088462
1104	50-51	0.38336033302763894
1104	52-53	0.39742618999593304
1104	54-55	0.3845797272107987
1104	56-57	0.39334519576387095
1104	58-59	0.3910308200470496
1104	60-61	0.4245763277411996
1104	62-63	0.386111094830909
1104	64-65	0.38089016736025627
1104	66-67	0.3240807312470224
1104	68-69	0.36502395744133054
1104	70-71	0.41704015770937275
1104	72-73	0.3676072485450632
1104	74-75	0.34158884610152285
1104	76-77	0.35111556876758954
1104	78-79	0.3011855812651305
1104	80-81	0.3391754897510282
1104	82-83	0.2898521360348383
1104	84-85	0.25102522266022476
1104	86-87	0.27411403064773765
1104	88-89	0.31498297384407437
1104	90-91	0.3146762235364946
1104	92-93	0.24658948217235377
1104	94-95	0.21629348155333616
1104	96-97	0.24471786644509308
1104	98-99	0.2403496103951568
1104	100	0.3258487519607094
1105	1	0.1917520716327381
1105	2	-0.3168440003526918
1105	3	0.06454817225314713
1105	4	0.162538582882938
1105	5	0.14872179736347846
1105	6	0.256597647991768
1105	7	0.30909714323354365
1105	8	0.2593525077705081
1105	9	0.3726023470651967
1105	10-11	0.36181466655401806
1105	12-13	0.33504539071677186
1105	14-15	0.43043790577440433
1105	16-17	0.48031551290939234
1105	18-19	0.43596899633677566
1105	20-21	0.42978742711001416
1105	22-23	0.43138380030237045
1105	24-25	0.5019488330852795
1105	26-27	0.5023434012570931
1105	28-29	0.43354582086242033
1105	30-31	0.47922556127708305
1105	32-33	0.488957602741138
1105	34-35	0.5122334688396961
1105	36-37	0.4881628568820844
1105	38-39	0.5238563087751444
1105	40-41	0.5752231860393948
1105	42-43	0.5579790134448288
1105	44-45	0.6016038226058953
1105	46-47	0.5327368123671548
1105	48-49	0.5248064057656805
1105	50-51	0.5131707029008297
1105	52-53	0.5626202568813099
1105	54-55	0.5225065388075976
1105	56-57	0.6102403883398964
1105	58-59	0.5736087599022
1105	60-61	0.56609613749999
1105	62-63	0.5273065574341516
1105	64-65	0.503735831458819
1105	66-67	0.5149384309240546
1105	68-69	0.5010158825009157
1105	70-71	0.550649688718714
1105	72-73	0.4520081209284328
1105	74-75	0.4766985946692799
1105	76-77	0.46706266047134903
1105	78-79	0.4990690941588056
1105	80-81	0.46711576289931145
1105	82-83	0.39991783549211846
1105	84-85	0.41265724319869435
1105	86-87	0.4499608961942414
1105	88-89	0.4225761109409092
1105	90-91	0.4003223342169946
1105	92-93	0.39855070263327264
1105	94-95	0.44612598545764115
1105	96-97	0.38243139913598156
1105	98-99	0.3348784939124272
1105	100	0.43227970313884967
1106	1	0.28742028012135634
1106	2	0.3523320540953776
1106	3	0.2707321469428834
1106	4	0.22553509561235074
1106	5	0.19574124332995524
1106	6	0.1924527561322975
1106	7	0.23342982133914347
1106	8	0.24799781177647873
1106	9	0.24385708555185204
1106	10-11	0.2778607838304623
1106	12-13	0.2534722830363094
1106	14-15	0.3788421160585713
1106	16-17	0.42030601397090805
1106	18-19	0.3883729646046916
1106	20-21	0.3587483666442921
1106	22-23	0.3202757731901613
1106	24-25	0.30818167029859467
1106	26-27	0.27533030322533847
1106	28-29	0.36293518109284406
1106	30-31	0.3299036760582865
1106	32-33	0.3294367889434966
1106	34-35	0.3330448902499086
1106	36-37	0.2951319766457914
1106	38-39	0.3168909730115317
1106	40-41	0.3390844076863502
1106	42-43	0.30182482153152534
1106	44-45	0.29590404018090766
1106	46-47	0.2763286231919153
1106	48-49	0.2502741041830703
1106	50-51	0.30325709192239714
1106	52-53	0.2679803878576337
1106	54-55	0.20321925073157132
1106	56-57	0.17806694255553168
1106	58-59	0.2566216247037687
1106	60-61	0.21761518889210407
1106	62-63	0.22472313371080332
1106	64-65	0.1728323120791444
1106	66-67	0.17508994468384032
1106	68-69	0.22237858979223546
1106	70-71	0.24251681420454219
1106	72-73	0.21579790584546998
1106	74-75	0.2109132239530851
1106	76-77	0.11976453637691264
1106	78-79	0.14929524557312135
1106	80-81	0.1660984060203745
1106	82-83	0.21614387747994357
1106	84-85	0.2685935705580391
1106	86-87	0.2460953454115682
1106	88-89	0.20388646524312293
1106	90-91	0.16322717230572437
1106	92-93	0.14966104207904607
1106	94-95	0.15556809405025973
1106	96-97	0.1486146889117066
1106	98-99	0.19837832857459148
1106	100	0.19701783435798248
1107	1	0.09961985058499323
1107	2	-0.5527285236474277
1107	3	-0.034709603335826955
1107	4	0.0806924010094292
1107	5	0.12175237520745696
1107	6	0.16648629931545855
1107	7	0.1671213748836351
1107	8	0.17255229075870915
1107	9	0.24939269444423928
1107	10-11	0.21638052282973774
1107	12-13	0.24174507961313907
1107	14-15	0.24067649864104368
1107	16-17	0.35747812361744735
1107	18-19	0.2768703660736378
1107	20-21	0.24672811121770621
1107	22-23	0.2508689678258733
1107	24-25	0.2921666857094465
1107	26-27	0.3572379283155058
1107	28-29	0.290595601448274
1107	30-31	0.2668144980794054
1107	32-33	0.31980500981043747
1107	34-35	0.3284771726946545
1107	36-37	0.32530055327376317
1107	38-39	0.36117318004149723
1107	40-41	0.3383007497415633
1107	42-43	0.37645819913005596
1107	44-45	0.3490905196221803
1107	46-47	0.2883861761462114
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2216	80-81	-0.6525444873802471
2216	82-83	-0.6209945623250732
2216	84-85	-0.6607627269373637
2216	86-87	-0.65027553217422
2216	88-89	-0.6139476017318728
2216	90-91	-0.7168884240447468
2216	92-93	-0.6640110050341477
2216	94-95	-0.579817688580448
2216	96-97	-0.6456690194741235
2216	98-99	-0.6305955680956572
2216	100	-0.6090200497805647
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	10.0
13	44.0
14	195.0
15	623.0
16	1591.0
17	3133.0
18	5651.0
19	8742.0
20	12892.0
21	18304.0
22	25448.0
23	30252.0
24	32030.0
25	34021.0
26	39482.0
27	49107.0
28	61805.0
29	78201.0
30	95849.0
31	119463.0
32	148182.0
33	187309.0
34	255644.0
35	351733.0
36	523990.0
37	881701.0
38	751073.0
39	280294.0
40	557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05226196635899	21.912820995259192	13.763427031906621	24.2714900064752
2	14.941523423153862	20.44053321911879	40.21189065425518	24.40605270347217
3	18.059190693601852	26.76269935580712	29.845919069360182	25.332190881230847
4	12.262646488370388	17.525980861830686	39.153623582972976	31.057749066825945
5	12.559594617752609	38.92412592267366	35.23010395988207	13.28617549969166
6	29.317409029231527	39.2508195474577	17.848772980350873	13.582998442959898
7	25.825218284514623	32.38428581649741	24.13595556607375	17.654540332914216
8	26.03186428534261	35.23135954817818	19.730605044169504	19.006171122309702
9	25.92096520475678	14.676253737496648	21.763187809456717	37.63959324828986
10-11	25.586166559261585	26.07207864853722	27.395012368262	20.94674242393919
12-13	25.945043789251223	23.808241405258713	27.8169567270937	22.429758078396368
14-15	23.272901859129107	24.764853655723485	25.56179304419663	26.40045144095078
16-17	21.750517703877758	27.28295370467081	26.99571639521742	23.970812196234007
18-19	21.107011984005318	27.4514875937126	29.433986903251373	22.007513519030713
20-21	22.827401557665716	26.679837966741527	28.58179621259454	21.910964262998213
22-23	23.24519631881428	26.1606900059265	28.352332412461518	22.241781262797705
24-25	22.720442595709258	26.298458157356386	29.007505639705528	21.973593607228832
26-27	22.610696340210897	26.5413418813928	28.01157675497874	22.836385023417563
28-29	21.705236598047495	27.102792165166328	28.744889085909385	22.447082150876785
30-31	22.694622507835234	26.580830995104733	28.57914086609855	22.14540563096148
32-33	23.101844532147474	26.832974426917183	27.687807955714415	22.377373085220928
34-35	22.96711937133691	26.704234449784703	28.493926412475595	21.834719766402788
36-37	22.735875064543116	26.255513683140336	28.112041343617538	22.89656990869901
38-39	22.859607717955594	26.247345726945596	28.816812130503177	22.076234424595633
40-41	22.40308417487865	25.521294450841804	29.4225815417322	22.653039832547346
42-43	22.586364352188713	25.706687360663572	28.494522982982616	23.212425304165105
44-45	22.821907534232867	26.546620642589247	28.183601645899458	22.44787017727842
46-47	22.785483202779456	26.50367945787786	27.383792373305365	23.327044966037313
48-49	23.73474064888251	26.17878854817861	27.50505778521168	22.581413017727204
50-51	23.082031296906333	26.244431280095103	27.28011061389008	23.393426809108483
52-53	22.42142751357907	26.743989985310186	27.22628715982276	23.60829534128798
54-55	22.610121828133735	26.71715726932965	26.88906030966063	23.78366059287599
56-57	23.68949169231508	26.075776213230707	26.724410311601037	23.510321782853175
58-59	22.343286759593997	26.59681481353618	27.74076962122344	23.31912880564639
60-61	22.58457157838594	27.20727666809095	26.88596170838291	23.322190045140196
62-63	23.082356514151453	26.374830887032534	26.45075660541242	24.092055993403594
64-65	22.178002405606946	26.560004583061485	26.956682063618498	24.30531094771307
66-67	22.85123962807155	26.24864659592608	26.950015110093545	23.95009866590883
68-69	22.350283475253377	26.151384854009464	27.332930974635765	24.165400696101393
70-71	22.24809808645357	25.789659994646374	27.514240898208538	24.448001020691517
72-73	22.863760492008662	25.90242782178495	27.756478827857006	23.477332858349378
74-75	23.04551065696199	26.35440551179773	27.198121539788005	23.40196229145228
76-77	22.69379478910889	26.341718775994728	26.667433869416257	24.29705256548013
78-79	22.511336385055337	26.393726174578582	26.911950198458662	24.182987241907426
80-81	22.067058917566143	26.91628224002057	27.49378844344641	23.522870398966873
82-83	23.022946619249133	25.786630351750095	28.469533658781632	22.72088937021914
84-85	23.385678198665477	26.28458117311108	26.93166588317825	23.398074745045196
86-87	21.74470038704125	26.996752556298613	27.5438517484407	23.714695308219436
88-89	22.827160042872112	25.643106686908496	27.446945262001236	24.082788008218156
90-91	22.409894343896525	25.754730682200705	27.5523592710313	24.283015702871467
92-93	22.73071740273699	26.12463365779773	27.255209352340508	23.88943958712477
94-95	21.561288348646126	25.990113469075602	28.01478964384697	24.433808538431297
96-97	21.924856084240442	25.898457143000105	27.586843560254483	24.589843212504974
98-99	21.325034952193956	26.620819261664064	27.45634462531761	24.597801160824375
100	22.060294814261407	25.80688854682948	26.690039428077554	25.442777210831558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	85.5
2	70.0
3	99.0
4	69.5
5	59.5
6	120.0
7	268.0
8	449.5
9	542.0
10	448.5
11	332.0
12	355.0
13	409.0
14	522.5
15	759.5
16	1001.5
17	1328.5
18	1836.0
19	2412.0
20	3090.5
21	3860.0
22	4746.0
23	5672.0
24	6596.0
25	7665.0
26	9439.0
27	11420.5
28	14214.5
29	20645.5
30	30391.5
31	39991.5
32	52200.0
33	65353.0
34	76344.5
35	87981.0
36	100342.0
37	121898.5
38	132085.0
39	124307.0
40	116253.5
41	108220.5
42	106683.0
43	113242.5
44	123986.5
45	148026.0
46	171359.0
47	186381.0
48	196099.5
49	190373.0
50	179825.0
51	182006.5
52	182575.5
53	173376.5
54	166028.5
55	144182.0
56	124386.5
57	102020.0
58	78614.0
59	76174.5
60	63083.5
61	41714.5
62	29692.5
63	24509.5
64	17738.5
65	7761.5
66	3854.5
67	2507.0
68	1759.5
69	1321.5
70	1007.0
71	730.5
72	567.5
73	517.5
74	420.0
75	305.0
76	225.5
77	142.0
78	76.0
79	37.0
80	25.0
81	14.0
82	11.0
83	9.5
84	4.0
85	1.5
86	1.5
87	0.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013509024028050739
2	0.00430287432004579
3	0.00195130347071844
4	0.025542062097481118
5	0.002826888361425432
6	0.0
7	5.503676455872522E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008730832195906866
16-17	0.00276434658351779
18-19	0.0
20-21	1.250835558152846E-5
22-23	7.254846237286507E-4
24-25	1.250835558152846E-5
26-27	1.8762533372292692E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.003342232611384E-4
36-37	0.0
38-39	0.0
40-41	4.753175120980815E-4
42-43	8.380598239624069E-4
44-45	0.0
46-47	0.0
48-49	6.25417779076423E-4
50-51	0.0
52-53	0.0
54-55	4.1277573419043923E-4
56-57	1.250835558152846E-4
58-59	2.751838227936261E-4
60-61	1.0006684465222768E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0016385945811802285
70-71	9.50635024196163E-4
72-73	0.0
74-75	2.876921783751546E-4
76-77	0.0030770554730560015
78-79	5.503676455872522E-4
80-81	0.0035398646295725545
82-83	0.001813711559321627
84-85	0.00617912765727506
86-87	0.00845564837311324
88-89	0.008105414416830442
90-91	0.0064167864133241005
92-93	0.009556383664287744
94-95	0.00178869484815857
96-97	0.00495330881028527
98-99	0.002063878670952196
100	0.08055380994504328
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3997328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.85323310001074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.04521016193404	15.321998160698874
2	15.998120762916566	7.312175659141079
3	6.4000091931957925	4.387827058029454
4	3.212846897332585	2.9369575623755138
5	1.8178522936575527	2.077190110417262
6	1.13139873250078	1.5513671377738216
7	0.7802059680469644	1.2481160197657768
8	0.5292047939655408	0.9675232411310128
9	0.3875843806992212	0.7971800578238345
>10	1.9890181824240687	8.522422557803255
>50	0.2645922716739886	4.301109918680076
>100	0.3303894073173422	16.94033561024452
>500	0.06439476292126395	10.486003978246632
>1k	0.04665334864703803	18.74927324302023
>5k	0.0019712682526917477	2.816666968322362
>10k+	5.475745146365967E-4	1.5838527165263843
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	17136	0.42868636249014347	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13494	0.3375755004342901	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11843	0.29627291030408315	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10410	0.26042396320742256	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10401	0.26019881280695506	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9109	0.2278772219842855	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7758	0.19407964520299562	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7039	0.17609262987675767	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6679	0.16708661385805718	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6466	0.16175805438032606	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6394	0.15995685117658595	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6272	0.156904812414693	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6092	0.15240180440534276	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6014	0.15045050093462434	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6008	0.15030040066764597	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5914	0.14794882981831864	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5898	0.14754856243970974	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5809	0.14532207514619766	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5576	0.1394931814452054	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5476	0.13699151032889972	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5428	0.13579070819307298	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5355	0.1339644882781698	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5255	0.13146281716186412	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4997	0.12500850568179545	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4979	0.12455820488086042	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4803	0.1201552637161624	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4652	0.11637774033054081	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4633	0.11590242281844271	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4608	0.1152770050393663	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4518	0.11302550103469118	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4461	0.11159954849839693	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4222	0.10562055453042633	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4201	0.10509520359600213	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4148	0.1037693179043601	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4145	0.10369426777087093	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4043	0.10114256323223914	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4040	0.10106751309874996	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	4035	0.10094242954293468	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	2.5016711163056923E-5
12-13	0.0	0.0	0.0	0.0	7.505013348917076E-5
14-15	0.0	0.0	0.0	0.0	7.505013348917076E-5
16-17	0.0	0.0	0.0	0.0	7.505013348917076E-5
18-19	0.0	0.0	0.0	0.0	7.505013348917076E-5
20-21	0.0	0.0	0.0	5.0033422326113846E-5	8.755848907069923E-5
22-23	0.0	0.0	0.0	7.505013348917076E-5	1.0006684465222769E-4
24-25	0.0	0.0	0.0	1.5010026697834152E-4	1.0006684465222769E-4
26-27	0.0	0.0	0.0	2.876921783751546E-4	1.0006684465222769E-4
28-29	0.0	0.0	0.0	0.001163277069082147	1.0006684465222769E-4
30-31	0.0	0.0	0.0	0.005178459210752783	1.1257520023375616E-4
32-33	0.0	0.0	0.0	0.014772367941785113	1.2508355581528462E-4
34-35	0.0	0.0	0.0	0.03032025392962499	1.2508355581528462E-4
36-37	0.0	0.0	0.0	0.058676696032950004	1.2508355581528462E-4
38-39	0.0	0.0	0.0	0.10478249470646392	1.5010026697834152E-4
40-41	0.0	0.0	0.0	0.1577803973054	1.5010026697834152E-4
42-43	0.0	0.0	0.0	0.21616940115997485	1.5010026697834152E-4
44-45	0.0	0.0	0.0	0.28656642637281704	1.5010026697834152E-4
46-47	0.0	0.0	0.0	0.37388725668746725	1.5010026697834152E-4
48-49	0.0	0.0	0.0	0.48386071896026545	1.5010026697834152E-4
50-51	0.0	0.0	0.0	0.6006637433805782	1.7511697814139845E-4
52-53	0.0	0.0	0.0	0.7203436895846426	1.7511697814139845E-4
54-55	0.0	0.0	0.0	0.8508558717223105	1.7511697814139845E-4
56-57	0.0	0.0	0.0	1.0129141266365933	1.7511697814139845E-4
58-59	0.0	0.0	0.0	1.208819991754492	1.7511697814139845E-4
60-61	0.0	0.0	0.0	1.4166463197415875	1.7511697814139845E-4
62-63	0.0	0.0	0.0	1.6361804685529933	1.7511697814139845E-4
64-65	0.0	0.0	0.0	1.86954885863757	1.8762533372292692E-4
66-67	0.0	0.0	0.0	2.1377905440834475	2.0013368930445538E-4
68-69	0.0	0.0	0.0	2.4402801071115503	2.0013368930445538E-4
70-71	0.0	0.0	0.0	2.7788187509256184	2.0013368930445538E-4
72-73	0.0	0.0	0.0	3.137245680114316	2.251504004675123E-4
74-75	0.0	0.0	0.0	3.488017495687119	2.751838227936261E-4
76-77	0.0	0.0	0.0	3.890361261322563	3.0020053395668305E-4
78-79	0.0	0.0	0.0	4.337672565273603	3.0020053395668305E-4
80-81	0.0	0.0	0.0	4.815666865466131	3.0020053395668305E-4
82-83	1.2508355581528461E-5	0.0	0.0	5.323943894521541	3.0020053395668305E-4
84-85	2.5016711163056923E-5	0.0	0.0	5.81454661714025	3.0020053395668305E-4
86-87	2.5016711163056923E-5	0.0	0.0	6.345713936909856	3.2521724511974E-4
88	2.5016711163056923E-5	0.0	0.0	6.779778892300056	3.502339562827969E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8080	0.0	41.142498	1
GGGCACC	4120	0.0	38.248554	94
GTATCAA	13560	0.0	36.547993	1
GTGGTAT	2090	0.0	33.29646	1
CAACGCA	15205	0.0	31.465311	5
ATCAACG	15450	0.0	31.006851	3
GTACTAG	4610	0.0	30.394705	1
CTGTGCG	5755	0.0	28.75185	9
TATCAAC	17255	0.0	28.69181	2
CAGTACG	645	0.0	28.423225	9
AACGCAG	17850	0.0	27.723623	6
TAGTACT	6120	0.0	27.423183	4
ACTGTGC	6380	0.0	26.88636	8
TATTCGC	460	0.0	26.569538	9
TATTCTC	2350	0.0	26.198364	5
ACGCAGA	18420	0.0	25.896212	7
CGCAGAG	18445	0.0	25.784678	8
GTACTGT	6695	0.0	25.410769	6
TCAACGC	19295	0.0	25.192823	4
GCATCGT	5235	0.0	24.956743	8
>>END_MODULE