FastQCFastQC Report
Mon 16 Jan 2017
ERR1146458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107241
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125740.30614224974867554No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC116660.2840349519300182No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA114220.2780942243223614No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT102160.24873144770418879No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC96950.23604653342718385No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA87650.21340359623406563No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC85750.20877761981826728No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC71820.17486190851717734No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53780.13093947981138676No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT50920.12397616794339557No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG50810.12370834825616514No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT50680.12339183408034736No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT50460.12285619470588652No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC47980.11681807812105498No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT46320.11277643556830486No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA45880.11170515681938314No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA44770.10900261270278516No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC43750.10651919378483025No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG43150.10505835912720972No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC42540.10357317722529552No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG41830.10184452288044457No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG37200.036.746821
GGGCACC35700.035.0178394
GCATCGT40950.032.6793258
TAGGCAT50050.030.9779345
GTATCAA88700.030.7694421
CAACGCA82800.029.6198445
GGTATCA59700.029.5298981
CTAGGCA54650.028.8024314
ATCAACG88100.027.7880173
TATTCTC24800.026.901675
CTAACGC18300.026.4473363
TAACGCC18650.025.9497344
TCTAACG18600.025.7687682
TATCAAC105400.025.5458552
CTGTGCG54100.025.346369
CATCGTC54400.025.0339329
TAGTACT54650.025.0194264
AACGCAG102100.024.9700286
CGCCTAC19650.024.8515197
CGACGAG25900.024.65668124-25
Produced by FastQC (version 0.11.5)