##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146458_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4107241 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.3859091784485 31.0 30.0 34.0 26.0 34.0 2 30.47950412454492 31.0 30.0 34.0 26.0 34.0 3 30.67266590881811 31.0 31.0 34.0 27.0 34.0 4 34.14972849170526 37.0 35.0 37.0 30.0 37.0 5 34.368622634999994 37.0 35.0 37.0 30.0 37.0 6 34.582764683153485 37.0 35.0 37.0 30.0 37.0 7 34.480341426276176 37.0 35.0 37.0 30.0 37.0 8 34.41425229247565 37.0 35.0 37.0 30.0 37.0 9 35.803892929584606 39.0 35.0 39.0 30.0 39.0 10-11 35.71942138286991 39.0 35.0 39.0 29.5 39.0 12-13 35.709063578202496 39.0 35.0 39.0 30.0 39.0 14-15 36.726147917787145 40.0 36.0 41.0 29.5 41.0 16-17 36.55664520294767 40.0 36.0 41.0 28.0 41.0 18-19 36.51219139563517 40.0 36.0 41.0 27.5 41.0 20-21 36.84920558593956 40.0 36.0 41.0 30.0 41.0 22-23 36.576523875759904 40.0 36.0 41.0 28.0 41.0 24-25 36.69608004497423 40.0 36.0 41.0 29.5 41.0 26-27 36.77218429597873 40.0 36.0 41.0 30.0 41.0 28-29 36.603108144859284 40.0 35.5 41.0 29.5 41.0 30-31 36.54679357748912 39.0 35.0 41.0 29.0 41.0 32-33 36.64894292786812 39.5 35.0 41.0 30.0 41.0 34-35 36.69710238576212 40.0 35.0 41.0 30.0 41.0 36-37 36.63304649033256 39.5 35.0 41.0 30.0 41.0 38-39 36.47234676513991 39.0 35.0 41.0 29.0 41.0 40-41 36.44430153964669 39.0 35.0 41.0 29.0 41.0 42-43 36.28549237797344 39.0 35.0 41.0 28.5 41.0 44-45 36.11222728347326 39.0 35.0 41.0 28.0 41.0 46-47 35.989676768419486 39.0 34.5 41.0 28.0 41.0 48-49 35.87318202170265 39.0 34.0 40.5 28.0 41.0 50-51 35.661238651445096 38.0 34.0 40.0 27.5 41.0 52-53 35.48649494879896 38.0 34.0 40.0 27.0 41.0 54-55 35.30672122721798 38.0 34.0 40.0 27.0 41.0 56-57 35.170080109737896 37.5 33.0 40.0 27.0 41.0 58-59 34.92537253109813 37.0 33.0 40.0 26.5 41.0 60-61 34.6743982152496 36.5 33.0 40.0 26.0 41.0 62-63 34.40933585343544 36.0 33.0 39.5 26.0 41.0 64-65 34.12842307037741 35.5 33.0 39.0 26.0 41.0 66-67 33.838633038577484 35.0 32.0 39.0 26.0 41.0 68-69 33.558130993530696 35.0 32.0 38.5 25.0 40.0 70-71 33.48327794741044 35.0 32.0 38.0 26.0 40.0 72-73 33.27282669801942 35.0 32.0 37.0 25.5 40.0 74-75 33.002176400167414 35.0 32.0 37.0 25.0 39.5 76-77 32.762151161813975 35.0 32.0 36.0 25.0 39.0 78-79 32.537102765579135 35.0 31.5 36.0 25.0 39.0 80-81 32.30024534718075 35.0 31.0 36.0 24.5 38.0 82-83 32.066972817032166 35.0 31.0 35.0 24.0 37.0 84-85 31.874942205728857 35.0 31.0 35.0 24.0 37.0 86-87 31.69472061658909 34.5 31.0 35.0 24.0 36.5 88-89 31.67763274665402 35.0 31.0 35.0 24.0 36.0 90-91 31.58889933656194 35.0 31.0 35.0 24.0 36.0 92-93 31.450333325948 35.0 31.0 35.0 23.5 36.0 94-95 31.417767547606775 35.0 31.0 35.0 23.5 35.5 96-97 31.327700638944734 35.0 31.0 35.0 23.0 35.0 98-99 31.238938620840607 34.5 31.0 35.0 23.0 35.0 100 30.743913493267137 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7976811494099323 1101 2 1.0199516361829097 1101 3 0.9780785424140177 1101 4 1.0766508167266124 1101 5 0.9584525864174083 1101 6 0.7337773296010113 1101 7 0.79909472084546 1101 8 0.837259479019707 1101 9 0.9980056911267496 1101 10-11 1.0731533764837948 1101 12-13 0.969374929058425 1101 14-15 1.226745913197746 1101 16-17 1.3636473940289449 1101 18-19 1.4748543199627733 1101 20-21 1.1604723611487984 1101 22-23 1.4032708692070912 1101 24-25 1.2604870438321143 1101 26-27 1.2055796461892072 1101 28-29 1.3848054279459134 1101 30-31 1.4093429851260808 1101 32-33 1.189916486463055 1101 34-35 1.11642992809859 1101 36-37 1.117712941062436 1101 38-39 1.1818431207451212 1101 40-41 1.1324470741173016 1101 42-43 1.1157067039200186 1101 44-45 1.189393574889884 1101 46-47 1.1598000590044606 1101 48-49 1.1496939355822988 1101 50-51 1.2273897865805026 1101 52-53 1.2000306860472705 1101 54-55 1.1256841590164868 1101 56-57 1.069913581621293 1101 58-59 1.0718065413862092 1101 60-61 1.035007089013014 1101 62-63 1.0007434683969336 1101 64-65 0.9597695828449844 1101 66-67 0.9983363547348318 1101 68-69 1.027314727730122 1101 70-71 0.9395793161430106 1101 72-73 0.9222763950255626 1101 74-75 0.8668131006959143 1101 76-77 0.8366250039714416 1101 78-79 0.8629012428965197 1101 80-81 0.929368866955798 1101 82-83 0.8743766871806784 1101 84-85 0.8613660029885537 1101 86-87 0.9025329173844092 1101 88-89 0.8667924476028084 1101 90-91 0.842274116704953 1101 92-93 0.892193057314568 1101 94-95 0.828076868809422 1101 96-97 0.7935872085204139 1101 98-99 0.8237773720702926 1101 100 0.928873140133831 1102 1 0.8428815045383864 1102 2 1.0112480249650133 1102 3 0.9128107210560472 1102 4 1.0886143885911252 1102 5 0.9243448977637243 1102 6 0.7048418219044095 1102 7 0.7660188415876021 1102 8 0.7440843309079099 1102 9 0.9290455866068612 1102 10-11 0.9739625087379764 1102 12-13 0.8989824926965753 1102 14-15 1.131465751266525 1102 16-17 1.3285233169048922 1102 18-19 1.296282328324061 1102 20-21 1.038668117979583 1102 22-23 1.306494379232035 1102 24-25 1.1737214174965942 1102 26-27 1.0102177418543903 1102 28-29 1.2691229089000444 1102 30-31 1.2634526617632034 1102 32-33 1.1638329045359654 1102 34-35 0.9974064214721281 1102 36-37 0.9336075329681464 1102 38-39 1.0371037580215727 1102 40-41 1.0487582737592618 1102 42-43 1.0404073725135916 1102 44-45 1.0476800864861815 1102 46-47 1.0165507384131303 1102 48-49 0.9717496407689481 1102 50-51 1.0386488380696548 1102 52-53 1.0367558947376168 1102 54-55 1.0659388559786862 1102 56-57 1.0026472619829434 1102 58-59 0.9451652892223876 1102 60-61 1.005209772611778 1102 62-63 0.989317354752508 1102 64-65 0.8401811362994422 1102 66-67 0.9547562316561766 1102 68-69 0.9479466228289866 1102 70-71 0.8539139287615711 1102 72-73 0.9038238842574202 1102 74-75 0.9378269607477634 1102 76-77 0.8713796688013957 1102 78-79 0.8361831927351986 1102 80-81 0.8968510539982049 1102 82-83 0.9519529367579409 1102 84-85 0.8642916221284977 1102 86-87 0.879465934224644 1102 88-89 0.8156809591666523 1102 90-91 0.8382931926580532 1102 92-93 0.8637416670229463 1102 94-95 0.8518029911289169 1102 96-97 0.9041258332115021 1102 98-99 0.8935393391620146 1102 100 0.9187761518392961 1103 1 0.6404893684594875 1103 2 0.8708757707180759 1103 3 0.8073633882239584 1103 4 0.8503182523951125 1103 5 0.7465026207016052 1103 6 0.5605807777616931 1103 7 0.6008600598173928 1103 8 0.689775160864869 1103 9 0.8383448976224415 1103 10-11 0.8831907582045773 1103 12-13 0.7671436477852822 1103 14-15 0.9648246271094223 1103 16-17 1.152826648768965 1103 18-19 1.2160812500641711 1103 20-21 0.9392218643375827 1103 22-23 1.155083452939408 1103 24-25 1.040193564609865 1103 26-27 0.92594335765952 1103 28-29 1.108793643840393 1103 30-31 1.1506116492459242 1103 32-33 0.9796303519776828 1103 34-35 0.8996482989731476 1103 36-37 0.9129861881679773 1103 38-39 0.9816401427927204 1103 40-41 0.9012922165140012 1103 42-43 0.9042509285311056 1103 44-45 1.0188280301502317 1103 46-47 1.0528947177881989 1103 48-49 0.9200739415846826 1103 50-51 0.9815509597067518 1103 52-53 0.9964837823774175 1103 54-55 0.9905449161411326 1103 56-57 0.9268049176715891 1103 58-59 0.858523876854278 1103 60-61 0.8612179541444505 1103 62-63 0.8609856107732341 1103 64-65 0.9096243957493471 1103 66-67 0.9212786555778081 1103 68-69 0.9278634625175641 1103 70-71 0.8263374348545227 1103 72-73 0.7607482794203264 1103 74-75 0.7763001713443813 1103 76-77 0.841137794960197 1103 78-79 0.7652349076249791 1103 80-81 0.8098890259503975 1103 82-83 0.7066145400605066 1103 84-85 0.7591329577775809 1103 86-87 0.7888038639897594 1103 88-89 0.7581798522610335 1103 90-91 0.7397554136471207 1103 92-93 0.7634457246450204 1103 94-95 0.7334068258906932 1103 96-97 0.6962695824904941 1103 98-99 0.7320783884362605 1103 100 0.8137594580937559 1104 1 0.5796197542118975 1104 2 0.7532981803238954 1104 3 0.6924165410935146 1104 4 0.7752231668460041 1104 5 0.7030614518224425 1104 6 0.4986765037473475 1104 7 0.4893416514771616 1104 8 0.4890266338107949 1104 9 0.6430697202399216 1104 10-11 0.6914099501763005 1104 12-13 0.6481607298602583 1104 14-15 0.83664877273673 1104 16-17 0.9281833248357998 1104 18-19 0.876374822593391 1104 20-21 0.7971547170999216 1104 22-23 0.9972546102169133 1104 24-25 0.7961205702264067 1104 26-27 0.7821338357577474 1104 28-29 0.9812604146259147 1104 30-31 0.9849898806256547 1104 32-33 0.8856954593741477 1104 34-35 0.6975821127488828 1104 36-37 0.7040788032746192 1104 38-39 0.8058971670377346 1104 40-41 0.6999225795270405 1104 42-43 0.7401317758283668 1104 44-45 0.7866939579494385 1104 46-47 0.7792001176077363 1104 48-49 0.7099074832323851 1104 50-51 0.8409353730042355 1104 52-53 0.8558129732632409 1104 54-55 0.7616822092206235 1104 56-57 0.7109449495403481 1104 58-59 0.655897206645264 1104 60-61 0.6650982423426228 1104 62-63 0.5844719967423799 1104 64-65 0.644385439866916 1104 66-67 0.7528355484736053 1104 68-69 0.6676271674749756 1104 70-71 0.48562110249118007 1104 72-73 0.5374863318735095 1104 74-75 0.5949405594632182 1104 76-77 0.6353436260759153 1104 78-79 0.5530245770573927 1104 80-81 0.5930709007530055 1104 82-83 0.5307312949284722 1104 84-85 0.4998484765191833 1104 86-87 0.5572514942373097 1104 88-89 0.5492908665948413 1104 90-91 0.5719427521051834 1104 92-93 0.5593842485734477 1104 94-95 0.4884726907961934 1104 96-97 0.48677470829508707 1104 98-99 0.5521751965635566 1104 100 0.6763892700202696 1105 1 0.48119998392559893 1105 2 0.670967752723115 1105 3 0.5656766800994006 1105 4 0.7509562029979833 1105 5 0.6415821358763338 1105 6 0.3908610113597746 1105 7 0.4832258326902803 1105 8 0.47683052746395305 1105 9 0.5693716525083516 1105 10-11 0.6504055418642523 1105 12-13 0.5602416443760845 1105 14-15 0.7524821965501545 1105 16-17 0.8647002688087042 1105 18-19 0.9148585216391254 1105 20-21 0.7381381370806537 1105 22-23 0.9509416002370656 1105 24-25 0.7652260667597943 1105 26-27 0.6940599301957846 1105 28-29 0.9085642105808986 1105 30-31 0.9147500077369628 1105 32-33 0.7576642619402776 1105 34-35 0.7072282054627124 1105 36-37 0.6129803337911142 1105 38-39 0.6820070349794989 1105 40-41 0.6078434176817495 1105 42-43 0.5631097516524974 1105 44-45 0.646620406784777 1105 46-47 0.6280002346828795 1105 48-49 0.7094848700412868 1105 50-51 0.7651487528138361 1105 52-53 0.7120013108267713 1105 54-55 0.670112849317448 1105 56-57 0.6122965518207621 1105 58-59 0.6043956971526825 1105 60-61 0.6110158899049836 1105 62-63 0.5730723439419378 1105 64-65 0.4939914524418185 1105 66-67 0.5674393122624153 1105 68-69 0.5585707126760866 1105 70-71 0.5244200458667052 1105 72-73 0.5137826574261624 1105 74-75 0.4801271690603315 1105 76-77 0.5137793788062268 1105 78-79 0.5074930888672853 1105 80-81 0.610428756529366 1105 82-83 0.6206041912409361 1105 84-85 0.5643447189521495 1105 86-87 0.5623625900650815 1105 88-89 0.5716464766350065 1105 90-91 0.5491347161921105 1105 92-93 0.5966173145126952 1105 94-95 0.5607652036804573 1105 96-97 0.635307334704418 1105 98-99 0.6810570720592892 1105 100 0.7272712831677381 1106 1 0.5046199338100372 1106 2 0.4791464936941807 1106 3 0.5696958863955288 1106 4 0.6970838742053118 1106 5 0.558237720563568 1106 6 0.2515389413200637 1106 7 0.2989028710890622 1106 8 0.336706077085104 1106 9 0.4961686492569086 1106 10-11 0.4091107243882064 1106 12-13 0.39672292278523 1106 14-15 0.5484729990575801 1106 16-17 0.5773484571377097 1106 18-19 0.6421451417524722 1106 20-21 0.4796940940223351 1106 22-23 0.7413733516541043 1106 24-25 0.557198225825573 1106 26-27 0.4387991919828522 1106 28-29 0.6686922141975842 1106 30-31 0.6461517695665648 1106 32-33 0.5558881958738766 1106 34-35 0.5528800970240226 1106 36-37 0.44067840553670123 1106 38-39 0.546768399186675 1106 40-41 0.4863360298288768 1106 42-43 0.5302188244465427 1106 44-45 0.5081768150106782 1106 46-47 0.4816075115458389 1106 48-49 0.48628069605251056 1106 50-51 0.4864841123232537 1106 52-53 0.4934859773697653 1106 54-55 0.5722338551269104 1106 56-57 0.5427019836617433 1106 58-59 0.534376811758662 1106 60-61 0.4874686673642259 1106 62-63 0.49068778314555317 1106 64-65 0.5054498064131465 1106 66-67 0.5459438369120448 1106 68-69 0.45399091081687004 1106 70-71 0.42217146220872337 1106 72-73 0.39259640932711193 1106 74-75 0.4822953846024802 1106 76-77 0.4601161066774466 1106 78-79 0.4965189023246026 1106 80-81 0.46024483319445153 1106 82-83 0.4524592810765071 1106 84-85 0.4114684374700772 1106 86-87 0.40116960822511416 1106 88-89 0.44335033822703096 1106 90-91 0.45120145032126047 1106 92-93 0.4597172948803312 1106 94-95 0.45951102129638954 1106 96-97 0.4428163038531885 1106 98-99 0.4608102606174427 1106 100 0.5161727082852785 1107 1 0.4015671151829352 1107 2 0.5649703196386824 1107 3 0.44963513245411946 1107 4 0.6551153792860305 1107 5 0.5258840174108741 1107 6 0.2971538957378712 1107 7 0.25823127655883127 1107 8 0.35649290237893183 1107 9 0.4205487322432191 1107 10-11 0.3720116186786555 1107 12-13 0.2988785426945384 1107 14-15 0.5156540690932019 1107 16-17 0.6508178196385472 1107 18-19 0.6919584888747252 1107 20-21 0.5081786311653502 1107 22-23 0.7234821378322351 1107 24-25 0.4230305353461432 1107 26-27 0.4012699850007664 1107 28-29 0.5637108975416609 1107 30-31 0.5572270013210883 1107 32-33 0.4765107249131688 1107 34-35 0.37478633090199054 1107 36-37 0.31044127251642806 1107 38-39 0.43124417242341195 1107 40-41 0.23643582704689692 1107 42-43 0.3800891043137895 1107 44-45 0.3146431836070107 1107 46-47 0.31023654249337085 1107 48-49 0.2895324037158602 1107 50-51 0.3569836405841329 1107 52-53 0.3960440242491856 1107 54-55 0.40243029404245334 1107 56-57 0.31386627057513294 1107 58-59 0.3426716103211618 1107 60-61 0.4104325940614899 1107 62-63 0.35951648893988164 1107 64-65 0.3879472582466832 1107 66-67 0.3934665920139224 1107 68-69 0.3330619614044181 1107 70-71 0.3638430367807075 1107 72-73 0.2520957695198689 1107 74-75 0.25337339847305174 1107 76-77 0.2522266144644192 1107 78-79 0.23251420662504074 1107 80-81 0.305692593416488 1107 82-83 0.2944513888325133 1107 84-85 0.26506875346992587 1107 86-87 0.36531704987850233 1107 88-89 0.41857851519783296 1107 90-91 0.4145939697466581 1107 92-93 0.5026422806200621 1107 94-95 0.47515499509124837 1107 96-97 0.4519617880823361 1107 98-99 0.47660884456467656 1107 100 0.4433822148403088 1108 1 0.32132175541654817 1108 2 0.42304405457691985 1108 3 0.48873796235463374 1108 4 0.5947719644997491 1108 5 0.5013188987802195 1108 6 0.23874965661013192 1108 7 0.25418441891801535 1108 8 0.33875555845620653 1108 9 0.3796321785287091 1108 10-11 0.4416873432606323 1108 12-13 0.272169392415627 1108 14-15 0.46221689927532594 1108 16-17 0.5552803852774701 1108 18-19 0.6757986677744654 1108 20-21 0.38873421545581976 1108 22-23 0.586894137302366 1108 24-25 0.4554802868418193 1108 26-27 0.3737645423686331 1108 28-29 0.5579549351188788 1108 30-31 0.64011355463618 1108 32-33 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-0.9068604072461071 2216 5 -0.9740575696961571 2216 6 -0.8614047647198646 2216 7 -1.0379063412879077 2216 8 -0.8331160466790593 2216 9 -1.1589943403650764 2216 10-11 -1.1529128078807886 2216 12-13 -1.2317817370452033 2216 14-15 -1.6941154168451718 2216 16-17 -1.4614002874570104 2216 18-19 -3.3037727509825814 2216 20-21 -1.6036061164791278 2216 22-23 -1.1197073413128962 2216 24-25 -1.3341233879780674 2216 26-27 -1.311816609719088 2216 28-29 -1.2699110454277545 2216 30-31 -1.4040769411830425 2216 32-33 -1.3356998228668289 2216 34-35 -1.5203711895749095 2216 36-37 -1.4933764184224714 2216 38-39 -1.5920738038205045 2216 40-41 -1.6333851546164055 2216 42-43 -1.6406545588449077 2216 44-45 -1.5769444698113304 2216 46-47 -1.7002646423814625 2216 48-49 -1.6814205601047618 2216 50-51 -1.4580926538641634 2216 52-53 -1.541370757423472 2216 54-55 -1.4890049450983511 2216 56-57 -1.4563714422981775 2216 58-59 -1.5014925135727566 2216 60-61 -1.5410200168596404 2216 62-63 -1.63002118174321 2216 64-65 -1.5335097181510164 2216 66-67 -1.5609782686372569 2216 68-69 -1.6268214944532957 2216 70-71 -1.681665011749871 2216 72-73 -1.6221006251034424 2216 74-75 -1.646574474920243 2216 76-77 -1.5945659882214471 2216 78-79 -1.7580725415906073 2216 80-81 -1.7083006234778466 2216 82-83 -1.759938179537329 2216 84-85 -1.6154582468618557 2216 86-87 -1.5789308798998505 2216 88-89 -1.5033712812583033 2216 90-91 -1.527935933767857 2216 92-93 -1.5399801935292778 2216 94-95 -1.4234300722750994 2216 96-97 -1.463494071879932 2216 98-99 -1.3505463123587127 2216 100 -1.3160769166945983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 17001.0 1 2711.0 2 436.0 3 439.0 4 439.0 5 213.0 6 213.0 7 263.0 8 500.0 9 786.0 10 1506.0 11 2587.0 12 4338.0 13 6458.0 14 8364.0 15 10762.0 16 13534.0 17 16168.0 18 18836.0 19 21451.0 20 24503.0 21 28406.0 22 32357.0 23 37683.0 24 42389.0 25 48020.0 26 54751.0 27 63028.0 28 74077.0 29 88336.0 30 105158.0 31 125172.0 32 154704.0 33 196199.0 34 268263.0 35 361372.0 36 528522.0 37 802295.0 38 700140.0 39 244539.0 40 322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3008313775174 21.173986303714443 12.778863613560937 23.746318705207226 2 15.219987908882276 19.91691269547796 41.224352841510495 23.638746554129273 3 18.48343029371902 26.78183668576517 29.219122316872088 25.51561070364372 4 12.000124260339108 17.014102325453972 40.32399660387687 30.661776810330043 5 12.990311569801518 38.64253406942678 35.27022873361329 13.096925627158415 6 29.66740473423451 38.61581748119151 18.48372377515444 13.233054009419536 7 26.67113498455543 32.03250468794085 23.817553637555797 17.478806689947923 8 25.8096636444836 35.458184977180686 20.27285296528772 18.459298413048 9 25.98250154313207 15.275465595130115 21.65977376533526 37.08225909640256 10-11 25.269493837352115 26.123935894509522 28.008058164674665 20.5985121034637 12-13 25.558881652903526 23.97093432330523 27.880936566676912 22.589247457114332 14-15 23.011301992635698 25.154726382954816 25.313359858730294 26.52061176567919 16-17 22.084813135527565 27.725702013102136 26.59743366867493 23.592051182695364 18-19 21.58368834974615 28.24755723128932 28.163954124646345 22.004800294318184 20-21 23.2465151822229 27.349112799710973 27.058776590797162 22.345595427268965 22-23 23.525016834160034 27.07498150199857 26.80720090982763 22.592800754013773 24-25 22.953979594134562 27.275907038858477 27.443610218793722 22.326503148213238 26-27 22.874678257788254 27.422151721856153 26.610974694751743 23.092195325603853 28-29 22.153359775109887 27.92382291653538 27.10128730817975 22.821530000174985 30-31 22.91368288635774 27.501186985741487 26.931996730282577 22.6531333976182 32-33 23.26146331327356 27.730069337716568 26.117916336820674 22.8905510121892 34-35 23.254559916209132 27.54966179032971 26.972791833964255 22.222986459496898 36-37 22.996342642429216 27.237582329716453 26.714782662768815 23.051292365085516 38-39 23.100719840081233 27.220265777315173 27.26376560130857 22.415248781295027 40-41 22.75159074128838 26.519870312233774 27.84966197359634 22.87887697288151 42-43 22.712534700292288 26.714010839710355 27.21544536325759 23.358009096739764 44-45 22.842779342819554 27.59099490752421 26.983413495054727 22.58281225460151 46-47 22.811373180701526 27.295618994504906 26.622475958434084 23.270531866359484 48-49 23.391505205608535 26.87188053564381 26.939310222450658 22.797304036296996 50-51 23.047673670434087 26.91986124895766 26.77934038544649 23.253124695161766 52-53 22.561090682209983 27.35416942544542 26.706406135148626 23.378333757195975 54-55 22.598141621212484 27.27503056254171 26.664293795987632 23.46253402025818 56-57 23.456663359486857 26.76221140552368 26.524006343812978 23.257118891176486 58-59 22.597161855178904 27.086846174078122 27.237667060476078 23.078324910266897 60-61 22.584427534875935 27.661944500297935 26.651478323997065 23.102149640829065 62-63 23.00967585139122 26.98612672736733 26.292352959316585 23.711844461924862 64-65 22.20947752578445 27.19514647364571 26.687369863043877 23.908006137525962 66-67 22.88295752721861 26.787726658572886 26.61632529176784 23.712990522440666 68-69 22.362075026954827 26.92555620950044 26.823110738547506 23.889258024997233 70-71 22.282823612177104 26.545481293524652 27.10621719835707 24.06547789594117 72-73 22.859871232369358 26.707772041906825 27.297645926591212 23.134710799132606 74-75 22.938381479317417 27.003041919843806 26.974842904312563 23.08373369652621 76-77 22.703459915983593 26.89240982590832 26.656272001012297 23.747858257095793 78-79 22.516938125941287 26.865623772416612 26.814675045173846 23.802763056468255 80-81 22.149491351681323 27.05706279766611 27.400028980299762 23.39341687035281 82-83 22.902967380186087 26.304698321365468 28.117832160044138 22.67450213840431 84-85 23.07354499005851 26.733775544487017 26.9330581906061 23.259621274848367 86-87 21.99974243317657 27.332419204368964 27.34430312644137 23.323535236013097 88-89 22.867503625468792 26.194195138542085 27.164778156761322 23.7735230792278 90-91 22.51552131824952 26.198419564793817 27.496288681441538 23.789770435515127 92-93 22.69648331075771 26.46449739646959 27.340632613887916 23.498386678884785 94-95 21.68124708892121 26.368256267497248 27.94140816943488 24.009088474146665 96-97 22.02428668434363 26.264874551044908 27.523751956841725 24.187086807769738 98-99 21.51645056719112 26.958972135395285 27.382748562806896 24.14182873460669 100 22.21728844923611 26.229102264724872 26.710333839482647 24.84327544655637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14200.0 1 8381.5 2 2366.0 3 1705.5 4 933.0 5 557.0 6 456.0 7 400.0 8 382.0 9 391.5 10 389.5 11 371.0 12 353.5 13 354.5 14 361.5 15 478.5 16 613.0 17 695.0 18 885.5 19 1125.0 20 1485.5 21 2011.0 22 2773.0 23 3741.0 24 4933.0 25 6701.0 26 9377.5 27 12633.0 28 17437.5 29 26047.0 30 37452.5 31 48486.0 32 62863.5 33 76657.0 34 87079.0 35 99291.5 36 110546.0 37 129501.5 38 138744.5 39 129327.5 40 119746.0 41 111881.5 42 110443.5 43 115778.5 44 125384.5 45 145200.5 46 163126.5 47 176377.0 48 186404.0 49 182912.0 50 173899.0 51 176181.0 52 177233.0 53 169986.0 54 161481.5 55 142091.0 56 125024.5 57 104752.5 58 84357.0 59 77945.5 60 65309.5 61 47808.5 62 36320.0 63 29257.0 64 21402.0 65 12083.0 66 7671.0 67 5580.0 68 4089.0 69 2991.5 70 2321.5 71 1798.0 72 1282.0 73 994.5 74 786.0 75 581.5 76 419.0 77 291.0 78 189.0 79 116.0 80 74.0 81 58.5 82 41.5 83 28.5 84 25.0 85 18.0 86 15.0 87 17.0 88 13.0 89 10.0 90 10.0 91 9.0 92 9.0 93 6.5 94 4.0 95 4.5 96 4.0 97 2.0 98 1.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9918336907914583 2 0.44528675088703096 3 0.47869117005795375 4 0.46381500379451807 5 0.4315305578611043 6 0.48672576067486667 7 0.5037931789247332 8 0.506203556109807 9 0.520909291663187 10-11 0.5342637551582681 12-13 0.5325351008134172 14-15 0.5288830141693657 16-17 0.5345194012233516 18-19 0.5323890173476551 20-21 0.530891661823594 22-23 0.4945655733374302 24-25 0.5295282161431482 26-27 0.5087356695163493 28-29 0.5151024738991454 30-31 0.5176467609278346 32-33 0.5202884369336983 34-35 0.5315368637973764 36-37 0.5501990265484786 38-39 0.5397053642579045 40-41 0.5442339516965281 42-43 0.54199400522151 44-45 0.5523294104241752 46-47 0.5501625056820382 48-49 0.5235509676690508 50-51 0.537648022115089 52-53 0.5307212311135383 54-55 0.5399001422122539 56-57 0.5279821661304998 58-59 0.533971588226744 60-61 0.5271543598245148 62-63 0.5210066806403618 64-65 0.5327542260120601 66-67 0.5307942728464193 68-69 0.5509537911215826 70-71 0.5352619921743087 72-73 0.5388166898411854 74-75 0.5354445965065113 76-77 0.5232588007375267 78-79 0.5537172033489148 80-81 0.5285056318828139 82-83 0.5287734515700442 84-85 0.537794105580851 86-87 0.8402599214411814 88-89 1.1042083968289176 90-91 1.0957599030590122 92-93 1.0948590550201462 94-95 1.0998380664782028 96-97 1.092984317209533 98-99 1.0888452856796083 100 1.0844262608403064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4107241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.57623518520697 #Duplication Level Percentage of deduplicated Percentage of total 1 74.00211447883727 25.587145144228867 2 12.754808427390044 8.820265118553959 3 5.1437038873420295 5.3354974599540395 4 2.5483542770000476 3.524499872671268 5 1.449149379475551 2.5053064881621694 6 0.8935780872549811 1.8537939660765372 7 0.5862166997753816 1.418841653665062 8 0.4143477868250386 1.1461269220586028 9 0.28972889135353197 0.9015960858650097 >10 1.5292107895107212 9.723280830429273 >50 0.1541732342859443 3.7555833504819667 >100 0.181718484825568 13.570845146743011 >500 0.03371829553812937 8.143255642903643 >1k 0.018264076748529378 11.145202369544045 >5k 6.322180412952476E-4 1.4544485934739912 >10k+ 2.809857961312212E-4 1.1143113551885335 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12574 0.30614224974867554 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11666 0.2840349519300182 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11422 0.2780942243223614 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10216 0.24873144770418879 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 9695 0.23604653342718385 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8765 0.21340359623406563 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8575 0.20877761981826728 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7182 0.17486190851717734 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5378 0.13093947981138676 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5092 0.12397616794339557 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5081 0.12370834825616514 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5068 0.12339183408034736 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5046 0.12285619470588652 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4798 0.11681807812105498 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 4632 0.11277643556830486 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4588 0.11170515681938314 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4477 0.10900261270278516 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4375 0.10651919378483025 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 4315 0.10505835912720972 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4254 0.10357317722529552 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4183 0.10184452288044457 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7043071005572841E-4 2 0.0 0.0 0.0 0.0 1.7043071005572841E-4 3 0.0 0.0 0.0 0.0 1.7043071005572841E-4 4 0.0 0.0 0.0 0.0 1.7043071005572841E-4 5 0.0 2.4347244293675488E-5 0.0 0.0 1.947779543494039E-4 6 0.0 2.4347244293675488E-5 0.0 0.0 2.4347244293675488E-4 7 0.0 2.4347244293675488E-5 0.0 0.0 2.4347244293675488E-4 8 0.0 2.4347244293675488E-5 0.0 0.0 2.4347244293675488E-4 9 0.0 2.4347244293675488E-5 0.0 2.4347244293675488E-5 2.4347244293675488E-4 10-11 0.0 2.4347244293675488E-5 0.0 1.2173622146837744E-4 2.7999330937726814E-4 12-13 0.0 2.4347244293675488E-5 0.0 1.2173622146837744E-4 7.182437066634269E-4 14-15 0.0 2.4347244293675488E-5 0.0 1.2173622146837744E-4 8.521535502786421E-4 16-17 0.0 2.4347244293675488E-5 0.0 1.4608346576205293E-4 9.617161496001817E-4 18-19 0.0 2.4347244293675488E-5 0.0 1.5825708790889067E-4 0.0010225842603343704 20-21 0.0 2.4347244293675488E-5 0.0 1.8260433220256616E-4 0.001229535836830612 22-23 0.0 2.4347244293675488E-5 0.0 2.191251986430794E-4 0.0013147511918584762 24-25 0.0 2.4347244293675488E-5 0.0 3.1651417581778134E-4 0.0013147511918584762 26-27 0.0 2.4347244293675488E-5 0.0 6.330283516355627E-4 0.0014364874133268538 28-29 0.0 2.4347244293675488E-5 0.0 0.0013147511918584762 0.0014851819019142047 30-31 0.0 2.4347244293675488E-5 0.0 0.005429435477489634 0.0015460500126483934 32-33 0.0 2.4347244293675488E-5 0.0 0.015582236347952312 0.0016434389898230953 34-35 0.0 2.4347244293675488E-5 0.0 0.03016623567986393 0.0017895224555851482 36-37 0.0 2.4347244293675488E-5 0.0 0.05708211424652218 0.001801696077731986 38-39 0.0 2.4347244293675488E-5 0.0 0.10164974492609516 0.0018625641884661747 40-41 0.0 2.4347244293675488E-5 0.0 0.15231636030123386 0.0018747378106130125 42-43 0.0 2.4347244293675488E-5 0.0 0.20575856152585154 0.0018747378106130125 44-45 0.0 2.4347244293675488E-5 0.0 0.2756229790265533 0.0019356059213472012 46-47 0.0 2.4347244293675488E-5 0.0 0.3627861135979116 0.002020821276375065 48-49 0.0 2.4347244293675488E-5 0.0 0.4667488467319059 0.0021182102535497673 50-51 0.0 2.4347244293675488E-5 0.0 0.5785148716620232 0.0021912519864307938 52-53 0.0 2.4347244293675488E-5 0.0 0.6883452906707934 0.002312988207899171 54-55 0.0 2.4347244293675488E-5 0.0 0.8163265803004986 0.00237385631863336 56-57 0.0 2.4347244293675488E-5 0.0 0.9716498252720014 0.0024347244293675485 58-59 0.0 4.8694488587350975E-5 0.0 1.1594279468869737 0.0024834189179549 60-61 0.0 7.304173288102646E-5 0.0 1.3604631430198522 0.002507766162248575 62-63 0.0 7.304173288102646E-5 0.0 1.5695085825253496 0.0026051551394232772 64-65 0.0 7.304173288102646E-5 0.0 1.7903989563797205 0.0026903704944511416 66-67 0.0 8.521535502786421E-5 0.0 2.0475302033652274 0.00275123860518533 68-69 0.0 9.738897717470195E-5 0.0 2.338431078186062 0.00275123860518533 70-71 0.0 9.738897717470195E-5 0.0 2.661433794608108 0.0027634122273321677 72-73 0.0 9.738897717470195E-5 0.0 2.99746228672727 0.002897322070947383 74-75 0.0 9.738897717470195E-5 0.0 3.3343064115302705 0.0030190582924157603 76-77 0.0 9.738897717470195E-5 0.0 3.718652009950232 0.003067752781003111 78-79 0.0 9.738897717470195E-5 0.0 4.143900978783568 0.003104273647443624 80-81 0.0 9.738897717470195E-5 0.0 4.605183869171544 0.003177315380324651 82-83 0.0 9.738897717470195E-5 0.0 5.08601759672734 0.0034329614454082437 84-85 0.0 9.738897717470195E-5 0.0 5.557295030897871 0.003457308689701919 86-87 0.0 9.738897717470195E-5 0.0 6.06652981892224 0.0036155657776108098 88 0.0 9.738897717470195E-5 0.0 6.474029646665486 0.0037738228655197006 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 3720 0.0 36.74682 1 GGGCACC 3570 0.0 35.01783 94 GCATCGT 4095 0.0 32.679325 8 TAGGCAT 5005 0.0 30.977934 5 GTATCAA 8870 0.0 30.769442 1 CAACGCA 8280 0.0 29.619844 5 GGTATCA 5970 0.0 29.529898 1 CTAGGCA 5465 0.0 28.802431 4 ATCAACG 8810 0.0 27.788017 3 TATTCTC 2480 0.0 26.90167 5 CTAACGC 1830 0.0 26.447336 3 TAACGCC 1865 0.0 25.949734 4 TCTAACG 1860 0.0 25.768768 2 TATCAAC 10540 0.0 25.545855 2 CTGTGCG 5410 0.0 25.34636 9 CATCGTC 5440 0.0 25.033932 9 TAGTACT 5465 0.0 25.019426 4 AACGCAG 10210 0.0 24.970028 6 CGCCTAC 1965 0.0 24.851519 7 CGACGAG 2590 0.0 24.656681 24-25 >>END_MODULE