FastQCFastQC Report
Mon 16 Jan 2017
ERR1146458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107241
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC131420.31997148450748325No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA122690.29871634023910454No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC100720.2452254445258995No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT99950.2433507067152865No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC98090.23882211927666286No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA93390.22737891445863537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88320.21503486160174187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83950.2043951158454057No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC79150.19270843858444148No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC62510.15219462407976547No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG53710.13076904910133103No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52700.1283099774276698No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT51260.12480397424938054No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG50610.12322140337029165No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT48870.1189849828631921No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA47990.11684242536534867No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC47920.11667199465529292No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC47700.11613635528083208No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG47390.11538159070772812No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA46620.11350685289711511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46000.11199732375090724No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA45090.10978172452018277No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG44620.10863740403838003No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT44010.10715222213646583No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT43670.10632441583048086No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC42140.10259928745354849No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA41480.10099236933016592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.043.723691
GTATCAA150400.036.7041
GGGCACC39400.033.3084694
GTACTAG36250.031.1312371
CAACGCA173350.030.61035
ATCAACG173900.030.4690743
TATCAAC192300.028.6301172
AACGCAG194800.028.2268686
CTGTGCG52650.026.8705259
TAGTACT53750.026.8531284
TCAACGC202950.026.4784224
TAGGCAT50250.025.9084365
ACGCAGA205450.025.8029127
CGCAGAG206800.025.6344688
TATTCTC28550.025.5166155
TAACGCC18400.024.7849964
ACTGTGC61950.024.731028
CTAACGC18400.024.529483
GTACTGT59750.024.461796
CTAGGCA56100.024.3873754
Produced by FastQC (version 0.11.5)