##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4107241
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.853378216666613	31.0	31.0	34.0	30.0	34.0
2	31.807443488219953	31.0	31.0	34.0	30.0	34.0
3	31.98816358718663	33.0	31.0	34.0	30.0	34.0
4	35.73068587891483	37.0	35.0	37.0	35.0	37.0
5	35.64290554170062	37.0	35.0	37.0	33.0	37.0
6	35.67942105174739	37.0	35.0	37.0	33.0	37.0
7	35.597024620663845	37.0	35.0	37.0	33.0	37.0
8	35.57670392363146	37.0	35.0	37.0	33.0	37.0
9	37.16633355578599	39.0	37.0	39.0	33.0	39.0
10-11	37.11244117401438	39.0	37.0	39.0	33.0	39.0
12-13	37.053863408550896	39.0	37.0	39.0	33.0	39.0
14-15	38.24833276157888	40.0	37.5	41.0	33.0	41.0
16-17	38.24534937199935	40.0	37.5	41.0	33.0	41.0
18-19	38.30536009452574	40.0	38.0	41.0	33.0	41.0
20-21	38.322441634177295	40.0	38.0	41.0	33.0	41.0
22-23	38.29491646582219	40.0	38.0	41.0	33.0	41.0
24-25	38.22831810940727	40.0	37.0	41.0	33.0	41.0
26-27	38.088190588280554	40.0	37.0	41.0	33.0	41.0
28-29	38.021644334968414	40.0	37.0	41.0	33.0	41.0
30-31	37.89458884930298	40.0	37.0	41.0	33.0	41.0
32-33	37.77051285765798	40.0	36.5	41.0	33.0	41.0
34-35	37.673939878375776	40.0	36.0	41.0	32.0	41.0
36-37	37.59207214770207	40.0	36.0	41.0	32.0	41.0
38-39	37.482574190314125	40.0	36.0	41.0	32.0	41.0
40-41	37.362517441757134	40.0	36.0	41.0	31.0	41.0
42-43	37.24307034819724	39.5	36.0	41.0	31.0	41.0
44-45	37.054839611310854	39.0	35.0	41.0	31.0	41.0
46-47	36.812686180333706	39.0	35.0	41.0	30.5	41.0
48-49	36.55827671178779	39.0	35.0	41.0	30.0	41.0
50-51	36.366227718315045	39.0	35.0	41.0	30.0	41.0
52-53	36.18918490539026	38.5	35.0	41.0	29.0	41.0
54-55	36.00012721435144	38.0	35.0	40.0	29.0	41.0
56-57	35.75698297226776	38.0	34.5	40.0	28.5	41.0
58-59	35.495013319159995	37.5	34.0	40.0	28.0	41.0
60-61	35.244099506213544	37.0	34.0	40.0	28.0	41.0
62-63	34.979625373821506	36.5	34.0	40.0	28.0	41.0
64-65	34.713284903418135	36.0	34.0	39.0	27.0	41.0
66-67	34.48947979921314	35.5	33.0	39.0	27.0	41.0
68-69	34.30068481494025	35.0	33.0	39.0	27.0	41.0
70-71	34.0788078907471	35.0	33.0	38.5	26.5	40.5
72-73	33.86707585457002	35.0	33.0	37.5	26.0	40.0
74-75	33.676966849522586	35.0	33.0	37.0	26.5	39.5
76-77	33.50337343243311	35.0	33.0	36.5	27.0	39.0
78-79	33.369941890432045	35.0	33.0	36.0	27.0	39.0
80-81	33.20627533178599	35.0	33.0	36.0	27.0	38.5
82-83	33.00544258298941	35.0	33.0	35.5	27.0	37.0
84-85	32.846796060908034	35.0	33.0	35.0	27.0	37.0
86-87	32.69766553752264	35.0	33.0	35.0	26.0	36.5
88-89	32.57482054254912	35.0	33.0	35.0	26.0	36.0
90-91	32.476532056433996	35.0	33.0	35.0	26.0	36.0
92-93	32.39599794606647	35.0	33.0	35.0	26.0	36.0
94-95	32.342752227103304	35.0	33.0	35.0	26.0	35.5
96-97	32.281274461372	35.0	33.0	35.0	26.0	35.0
98-99	32.239954144400095	35.0	33.0	35.0	26.0	35.0
100	31.745819639022887	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43144523994524775
1101	2	0.554947651089023
1101	3	0.48424427446383334
1101	4	0.320069127456037
1101	5	0.3641683538734952
1101	6	0.3917696969213509
1101	7	0.3678778346579321
1101	8	0.41502144493054516
1101	9	0.4826506176396137
1101	10-11	0.5246223689103502
1101	12-13	0.4954405671496147
1101	14-15	0.6341522395769346
1101	16-17	0.6334909511541227
1101	18-19	0.6353559774892759
1101	20-21	0.63803350917334
1101	22-23	0.622183906722114
1101	24-25	0.6507929338358664
1101	26-27	0.7262402528770906
1101	28-29	0.7147806716791365
1101	30-31	0.7689972736237323
1101	32-33	0.7296820419070755
1101	34-35	0.7227997676910078
1101	36-37	0.7230290293469679
1101	38-39	0.7602369813639029
1101	40-41	0.7299139925684273
1101	42-43	0.7440346141040735
1101	44-45	0.7597783813284522
1101	46-47	0.7636148240223264
1101	48-49	0.7518799998190673
1101	50-51	0.7548597820123462
1101	52-53	0.7032444046094568
1101	54-55	0.7067093661200019
1101	56-57	0.7733421401992757
1101	58-59	0.7946240484864404
1101	60-61	0.7224133159035588
1101	62-63	0.6777441314972847
1101	64-65	0.6604584251590992
1101	66-67	0.6803738580440566
1101	68-69	0.6450704422662028
1101	70-71	0.6143489932253345
1101	72-73	0.5642270436697459
1101	74-75	0.5205582154809463
1101	76-77	0.5293725275681567
1101	78-79	0.48260399273847554
1101	80-81	0.44237976763951536
1101	82-83	0.49035992910001625
1101	84-85	0.49186712516471687
1101	86-87	0.4935851436840366
1101	88-89	0.5099700190443244
1101	90-91	0.5297253628614484
1101	92-93	0.5401760361155397
1101	94-95	0.5235023693593632
1101	96-97	0.5122144126494916
1101	98-99	0.5235831645652098
1101	100	0.5844159818912509
1102	1	0.4229763794187633
1102	2	0.5096207856010153
1102	3	0.41370009466223223
1102	4	0.28906197748708706
1102	5	0.33539004507246517
1102	6	0.2947765218626728
1102	7	0.333353078323249
1102	8	0.3060879507698644
1102	9	0.42366101660719124
1102	10-11	0.40421541234817226
1102	12-13	0.42327730516009865
1102	14-15	0.5731611122945068
1102	16-17	0.5974503446760835
1102	18-19	0.5825892628733698
1102	20-21	0.577898773468462
1102	22-23	0.5650693599088683
1102	24-25	0.5731229066339765
1102	26-27	0.662070000238387
1102	28-29	0.6609026411063468
1102	30-31	0.6344265228137544
1102	32-33	0.6310732470223783
1102	34-35	0.640184197309047
1102	36-37	0.6312503757151333
1102	38-39	0.657534742058985
1102	40-41	0.6514148766205778
1102	42-43	0.7520963085379933
1102	44-45	0.735927396784767
1102	46-47	0.7044929760971783
1102	48-49	0.6529716914664832
1102	50-51	0.6584158448699355
1102	52-53	0.5886246262440622
1102	54-55	0.6261775243363985
1102	56-57	0.738235515423348
1102	58-59	0.6537737230691363
1102	60-61	0.6598586192096505
1102	62-63	0.5783008818445055
1102	64-65	0.6333017537946048
1102	66-67	0.6181750180502732
1102	68-69	0.6350826014167978
1102	70-71	0.6031451223302966
1102	72-73	0.5949695976433063
1102	74-75	0.5522300494991512
1102	76-77	0.5120003375910471
1102	78-79	0.5548645841482127
1102	80-81	0.5270546512191956
1102	82-83	0.5166160158619562
1102	84-85	0.5509836198439828
1102	86-87	0.525810010162715
1102	88-89	0.505026928902943
1102	90-91	0.46786306188815985
1102	92-93	0.481649484463162
1102	94-95	0.4842946982985623
1102	96-97	0.47953462248140255
1102	98-99	0.4664144975860367
1102	100	0.5391025059060546
1103	1	0.2914361150072793
1103	2	-0.10341652300838788
1103	3	0.22032171959129698
1103	4	0.20339857637308256
1103	5	0.2653941905836419
1103	6	0.3218192535662112
1103	7	0.33067058109536873
1103	8	0.3453573099525187
1103	9	0.39885939231707823
1103	10-11	0.42862036527713343
1103	12-13	0.4061893662644991
1103	14-15	0.5026669501303758
1103	16-17	0.5192080337911094
1103	18-19	0.5034092369023497
1103	20-21	0.470677418039557
1103	22-23	0.43768036327125515
1103	24-25	0.48055319216644676
1103	26-27	0.5372005243683944
1103	28-29	0.5565253488090747
1103	30-31	0.5602692216703886
1103	32-33	0.5944633968089903
1103	34-35	0.5142849770723075
1103	36-37	0.5122360817455558
1103	38-39	0.5561023486213799
1103	40-41	0.575992039436386
1103	42-43	0.5854144688581826
1103	44-45	0.6304099173592661
1103	46-47	0.5780788264923373
1103	48-49	0.6528999437008238
1103	50-51	0.585310628258398
1103	52-53	0.5750134803773435
1103	54-55	0.5695720445607648
1103	56-57	0.6289892870798752
1103	58-59	0.5891818248939273
1103	60-61	0.5893182517894147
1103	62-63	0.6042668633390704
1103	64-65	0.5588185759770354
1103	66-67	0.6063042320401522
1103	68-69	0.5290278905812613
1103	70-71	0.5258644297455248
1103	72-73	0.5124036261065044
1103	74-75	0.5350522924654513
1103	76-77	0.4969383771813227
1103	78-79	0.5026423515056635
1103	80-81	0.4470791857527061
1103	82-83	0.46183545308650764
1103	84-85	0.44999509422549266
1103	86-87	0.45684035458782546
1103	88-89	0.41205040231673706
1103	90-91	0.45152996133477785
1103	92-93	0.4868558484976049
1103	94-95	0.47112223550274734
1103	96-97	0.4587724507703186
1103	98-99	0.5011978677501432
1103	100	0.435021506719675
1104	1	0.3394691770336209
1104	2	0.4105795638419991
1104	3	0.35227459649964743
1104	4	0.23613322506815848
1104	5	0.23165829254642034
1104	6	0.2500142416513498
1104	7	0.24944049087065423
1104	8	0.2052500884561681
1104	9	0.3445075806448372
1104	10-11	0.31731440123936494
1104	12-13	0.32561176852546225
1104	14-15	0.4468561094194854
1104	16-17	0.3981621767097252
1104	18-19	0.4408972814233394
1104	20-21	0.45680743019367753
1104	22-23	0.447437592145846
1104	24-25	0.49479356337975133
1104	26-27	0.5479120524066516
1104	28-29	0.5052173232664998
1104	30-31	0.5584239448768855
1104	32-33	0.4835104432124595
1104	34-35	0.5070948855309609
1104	36-37	0.559459132633684
1104	38-39	0.5203842553481337
1104	40-41	0.51522686058712
1104	42-43	0.4555259530894702
1104	44-45	0.4533043106671286
1104	46-47	0.46040327999757835
1104	48-49	0.46849537100815297
1104	50-51	0.48472185546187063
1104	52-53	0.5100353854733868
1104	54-55	0.37909855850247
1104	56-57	0.3634498352170894
1104	58-59	0.4162221650263973
1104	60-61	0.3923227095709976
1104	62-63	0.33797504630386044
1104	64-65	0.290297002598777
1104	66-67	0.37750034576726677
1104	68-69	0.3582149679631996
1104	70-71	0.377331067013003
1104	72-73	0.4231269281663472
1104	74-75	0.3776811676141065
1104	76-77	0.28867738879313976
1104	78-79	0.25034701700313633
1104	80-81	0.2927086558344314
1104	82-83	0.21710378368134542
1104	84-85	0.20187337957876395
1104	86-87	0.19401086900523978
1104	88-89	0.20970296016419354
1104	90-91	0.2771946872044637
1104	92-93	0.2872931689459364
1104	94-95	0.1982495292947135
1104	96-97	0.21784835799379465
1104	98-99	0.23076744874310862
1104	100	0.25803780727263614
1105	1	0.1138951926828149
1105	2	-0.37876735594119637
1105	3	0.049985363772854896
1105	4	0.1396775589913375
1105	5	0.1641880508385185
1105	6	0.21679150243876677
1105	7	0.2729351461404903
1105	8	0.2370694458685918
1105	9	0.31602640487536604
1105	10-11	0.31188903958840086
1105	12-13	0.3167935686482366
1105	14-15	0.3679108190709002
1105	16-17	0.33519948287138135
1105	18-19	0.3993118943444287
1105	20-21	0.4120198611822872
1105	22-23	0.3461949827935342
1105	24-25	0.3523146015259542
1105	26-27	0.3442865685160754
1105	28-29	0.38379211289012005
1105	30-31	0.42328942487174714
1105	32-33	0.4444369715482779
1105	34-35	0.38071257339252895
1105	36-37	0.41242524794110835
1105	38-39	0.45167472866880587
1105	40-41	0.4312660503612804
1105	42-43	0.4376885528285257
1105	44-45	0.4876606053060897
1105	46-47	0.4770596354920258
1105	48-49	0.5033464722931953
1105	50-51	0.5526385249242693
1105	52-53	0.48531337792958595
1105	54-55	0.5006384066463809
1105	56-57	0.48819739322041755
1105	58-59	0.39108663042426883
1105	60-61	0.3719301616943156
1105	62-63	0.3579046334767497
1105	64-65	0.47896387808129504
1105	66-67	0.4274996697081548
1105	68-69	0.43954496786763286
1105	70-71	0.3896899358613979
1105	72-73	0.3640748550437891
1105	74-75	0.34938165096078677
1105	76-77	0.33756524359964146
1105	78-79	0.24261690376476253
1105	80-81	0.4025743355971301
1105	82-83	0.34213704019809654
1105	84-85	0.3515531334281832
1105	86-87	0.3646762346901866
1105	88-89	0.3432919353378736
1105	90-91	0.36577074456599945
1105	92-93	0.39546587978438197
1105	94-95	0.396641057592916
1105	96-97	0.3657912117846749
1105	98-99	0.3465992739054755
1105	100	0.37338650432882403
1106	1	0.22109670819728677
1106	2	0.30266628596647394
1106	3	0.22674546228289927
1106	4	0.11884231195275419
1106	5	0.22458033589964543
1106	6	0.16472053314230095
1106	7	0.18301714078071285
1106	8	0.18327721752318382
1106	9	0.22425343125657804
1106	10-11	0.24926553680635521
1106	12-13	0.26258637896016523
1106	14-15	0.3218211543000322
1106	16-17	0.32057928757237875
1106	18-19	0.3368398244347972
1106	20-21	0.3075055130458253
1106	22-23	0.2824353144544176
1106	24-25	0.2604104077647307
1106	26-27	0.2925843189392907
1106	28-29	0.3352333680926449
1106	30-31	0.2620340537390007
1106	32-33	0.3104903119420612
1106	34-35	0.2924175401806153
1106	36-37	0.2906787970263238
1106	38-39	0.3164707647123066
1106	40-41	0.2630689073005783
1106	42-43	0.30469252943298386
1106	44-45	0.3507059887886115
1106	46-47	0.27394895388474794
1106	48-49	0.2619842623733959
1106	50-51	0.19869158414095978
1106	52-53	0.2370281078665002
1106	54-55	0.27509853663624995
1106	56-57	0.30152278805407917
1106	58-59	0.26596791815928356
1106	60-61	0.3090948755310947
1106	62-63	0.3387988756704843
1106	64-65	0.27955351443427645
1106	66-67	0.24292404898222486
1106	68-69	0.32280037962596
1106	70-71	0.26630883044120424
1106	72-73	0.27590678929598056
1106	74-75	0.20065714676805158
1106	76-77	0.2050098477113096
1106	78-79	0.197386004844617
1106	80-81	0.19151058264551324
1106	82-83	0.16339635681570996
1106	84-85	0.17561183810038727
1106	86-87	0.17677293068933153
1106	88-89	0.17020132287014178
1106	90-91	0.19946226598619177
1106	92-93	0.1898242666964549
1106	94-95	0.10992408324247549
1106	96-97	0.1771477791508076
1106	98-99	0.1350516662221608
1106	100	0.19975294991625248
1107	1	0.016074136487318924
1107	2	-0.5806178310856893
1107	3	-0.10320411078049929
1107	4	-0.0223893676048732
1107	5	0.04424389197793488
1107	6	0.11849934417963937
1107	7	0.12220720073946012
1107	8	0.06273881402973558
1107	9	0.15283928595881235
1107	10-11	0.17153481323396846
1107	12-13	0.13190587929925357
1107	14-15	0.1542459222686432
1107	16-17	0.2424659267441811
1107	18-19	0.2446385102677553
1107	20-21	0.079749169978939
1107	22-23	0.10064219588699075
1107	24-25	0.14841773015162119
1107	26-27	0.2509546927836084
1107	28-29	0.1703084169728868
1107	30-31	0.23346717982844467
1107	32-33	0.20487913105201017
1107	34-35	0.24095609746566282
1107	36-37	0.17398716971980122
1107	38-39	0.14726633602462869
1107	40-41	0.1719762986360891
1107	42-43	0.19855958141004493
1107	44-45	0.1746915269854199
1107	46-47	0.168359440565105
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2216	80-81	-0.4430202787393611
2216	82-83	-0.3997709910052265
2216	84-85	-0.3922297894953033
2216	86-87	-0.3511319308971892
2216	88-89	-0.4314685847799353
2216	90-91	-0.5084398661556975
2216	92-93	-0.5327436282388653
2216	94-95	-0.4520395906345698
2216	96-97	-0.5743752231046031
2216	98-99	-0.5109729867902288
2216	100	-0.4461687640412926
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	8.0
13	48.0
14	255.0
15	676.0
16	1795.0
17	3612.0
18	6497.0
19	9728.0
20	14270.0
21	20076.0
22	27924.0
23	33075.0
24	35861.0
25	37285.0
26	41322.0
27	49612.0
28	62276.0
29	78270.0
30	96880.0
31	120186.0
32	149843.0
33	188371.0
34	255962.0
35	350309.0
36	516312.0
37	873992.0
38	814167.0
39	317745.0
40	880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.21980066335161	21.503019075315805	14.032630899445081	24.2445493618875
2	14.816188241939784	20.383851689349182	40.348108316056155	24.451851752654882
3	17.926205076687914	26.484734242540963	30.215871479263317	25.373189201507806
4	12.187970935573334	17.560179904086223	38.92985416157273	31.321994998767718
5	12.52755584578368	38.6784004534571	35.32646038069554	13.467583320063675
6	29.497295142895197	39.0752332283399	17.795863451888994	13.631608176875911
7	25.85342943395087	32.670346849927554	23.744315806568085	17.731907909553488
8	26.037916937428314	35.27487186654009	19.865354869607117	18.821856326424477
9	25.97140026601799	14.713818838485496	21.461901066920593	37.85287982857592
10-11	25.279719402878964	26.20881024512562	27.653867888443852	20.857602463551565
12-13	25.81072062730188	23.999979548314794	28.085471488037832	22.103828336345494
14-15	23.03782371787536	24.972996624699835	25.958875490821043	26.030304166603763
16-17	21.57541792799652	27.542282195228907	27.21425324443702	23.668046632337557
18-19	21.141673936348027	27.20041263709629	29.832325397998318	21.82558802855737
20-21	22.57924755075823	26.39229429102094	29.171106488650956	21.857351669569873
22-23	22.84216432487102	26.048853340033745	28.918793200691027	22.190189134404207
24-25	22.325363912173646	26.266427998746604	29.584799138886662	21.823408950193084
26-27	22.266106857572137	26.477189572981423	28.73971007492026	22.516993494526183
28-29	21.558084855502756	26.977854477007803	29.20916985392384	22.254890813565602
30-31	22.40035342459817	26.49669206165404	28.982557877660454	22.12039663608734
32-33	22.794888344755034	26.746653045195057	28.278216447488713	22.180242162561196
34-35	22.632880825696482	26.62578503458602	28.979589252934495	21.761744886783006
36-37	22.52126183976056	26.275375611024533	28.731829955924184	22.47153259329073
38-39	22.628462756385613	26.210831066401997	29.255222666505325	21.905483510707064
40-41	22.21578505079407	25.628700570446465	29.723378688001123	22.43213569075834
42-43	22.369643229745552	25.837594337176412	28.87935578637464	22.913406646703393
44-45	22.531329425275995	26.666574958713163	28.545877877631238	22.256217738379608
46-47	22.6635471354128	26.45739560936405	27.9038897400956	22.975167515127552
48-49	23.408382980728177	26.24715607134714	27.85609322327821	22.488367724646473
50-51	22.889221742770875	26.443237686807276	27.519325016476998	23.14821555394485
52-53	22.316245869185668	26.885748851844827	27.45189532340566	23.346109955563847
54-55	22.448445689081332	26.85159214671133	27.25849734930325	23.44146481490409
56-57	23.279630963544466	26.33201180404391	27.14276375368207	23.245593478729553
58-59	22.32603640676207	26.672139128266814	27.89456839722816	23.107256067742956
60-61	22.446520021484023	27.17099666491204	27.283554057495575	23.098929256108363
62-63	22.802375122375338	26.471115281523534	26.998549634657426	23.727959961443705
64-65	22.075950741629235	26.645831106574946	27.32021325264332	23.958004899152495
66-67	22.608510189686946	26.35321375103141	27.27670472709052	23.761571332191124
68-69	22.24186263148777	26.29113879516072	27.543604447676184	23.923394125675326
70-71	22.15797459022251	25.986289681397185	27.78106536608834	24.07467036229196
72-73	22.7871094002032	26.071065710534153	27.884935410412975	23.256889478849672
74-75	22.811282596037692	26.509080271526166	27.45208843384292	23.22754869859322
76-77	22.605666981160788	26.44420367045074	27.04412453967191	23.90600480871656
78-79	22.29859453545021	26.449213744046002	27.344065089305573	23.90812663119822
80-81	21.979149077751416	26.782540563513237	27.770364479048947	23.467945879686404
82-83	22.724471637018897	25.963285641903532	28.528727952868728	22.78351476820884
84-85	22.977839910172886	26.356948337357156	27.249704498453315	23.41550725401665
86-87	21.808640479036143	26.98474170444544	27.6198536930471	23.586764123471312
88-89	22.56631727297608	25.929040810105082	27.51374264534722	23.99089927157162
90-91	22.18063740427626	25.871797937229623	27.86009791572975	24.08746674276437
92-93	22.49471496353191	26.245982958344516	27.42293537816317	23.836366699960408
94-95	21.44236871357669	26.05795054483319	28.07610195281452	24.423578788775593
96-97	21.742585264843317	25.996267629630722	27.6992087856916	24.56193831983436
98-99	21.219961559756463	26.64883030926038	27.58217734226669	24.54903078871647
100	21.920238251593545	25.94811711892379	26.945876078273894	25.185768551208763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	224.0
1	155.5
2	139.0
3	195.0
4	146.5
5	113.0
6	215.0
7	555.5
8	1017.0
9	1247.5
10	1048.5
11	758.0
12	724.0
13	831.5
14	1012.0
15	1392.5
16	1833.0
17	2304.0
18	2996.5
19	3841.5
20	4841.5
21	5960.5
22	7108.5
23	8274.5
24	9565.5
25	11239.0
26	13734.5
27	16692.0
28	20742.0
29	28459.5
30	39101.5
31	49444.0
32	63246.5
33	76278.0
34	85642.5
35	96863.0
36	107606.0
37	126920.5
38	136439.0
39	126749.5
40	117638.0
41	109794.0
42	107800.0
43	113326.5
44	122329.5
45	142894.5
46	162899.0
47	176232.5
48	185938.5
49	181806.0
50	172687.0
51	174476.5
52	174638.0
53	168552.0
54	163244.5
55	144194.5
56	126095.5
57	105563.5
58	84616.5
59	80066.5
60	67122.5
61	47701.5
62	35733.5
63	28389.0
64	20496.5
65	11240.0
66	6990.5
67	5076.0
68	3694.5
69	2766.0
70	2151.0
71	1661.5
72	1143.0
73	866.0
74	676.5
75	496.5
76	333.5
77	183.5
78	89.5
79	44.5
80	19.5
81	7.0
82	7.5
83	5.0
84	3.5
85	2.5
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012660567032711255
2	0.004528587438623641
3	0.0018260433220256616
4	0.025637648241240287
5	0.003092100025296787
6	0.0
7	4.382503972861588E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008448493769905394
16-17	0.003262530735352515
18-19	0.0
20-21	1.2173622146837744E-5
22-23	7.182437066634269E-4
24-25	0.0
26-27	1.8260433220256616E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.26076775139321E-4
36-37	0.0
38-39	0.0
40-41	4.74771263726672E-4
42-43	7.791118173976156E-4
44-45	0.0
46-47	0.0
48-49	6.452019737824004E-4
50-51	0.0
52-53	0.0
54-55	4.6259764157983427E-4
56-57	1.095625993215397E-4
58-59	2.3129882078991713E-4
60-61	7.304173288102646E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001521702768354718
70-71	0.0010347578824812082
72-73	0.0
74-75	3.0434055367094357E-4
76-77	0.0028242803380663564
78-79	5.478129966076984E-4
80-81	0.003226009868912002
82-83	0.001801696077731986
84-85	0.005855512252628954
86-87	0.007973722506178721
88-89	0.007718076441095129
90-91	0.005843338630482117
92-93	0.008838049678604203
94-95	0.00161909174552942
96-97	0.00485727523658826
98-99	0.0021182102535497673
100	0.08141718491805083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4107241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.628402418988703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.08096536941164	18.257115034613612
2	16.324651524775135	9.0204808335249
3	6.640242404168693	5.50377867906017
4	3.401706715685359	3.75934888209326
5	1.95782270822368	2.7045756823919067
6	1.228867088890015	2.03709806707827
7	0.8156116863558351	1.5773833521788307
8	0.5809385754112482	1.2840323793740784
9	0.4127577667181065	1.026345391240582
>10	2.021988306360954	10.199468498029509
>50	0.2130301968216662	4.156228855540458
>100	0.24559717156417668	14.821032790241222
>500	0.047200673970823455	9.177287166088599
>1k	0.02738695821801119	13.632956029883738
>5k	9.686705479039328E-4	1.9795727843599331
>10k+	2.641828767010726E-4	0.8632955743012082
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13142	0.31997148450748325	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12269	0.29871634023910454	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10072	0.2452254445258995	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9995	0.2433507067152865	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9809	0.23882211927666286	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9339	0.22737891445863537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8832	0.21503486160174187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8395	0.2043951158454057	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7915	0.19270843858444148	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6251	0.15219462407976547	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5371	0.13076904910133103	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5270	0.1283099774276698	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5126	0.12480397424938054	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5061	0.12322140337029165	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4887	0.1189849828631921	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4799	0.11684242536534867	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4792	0.11667199465529292	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4770	0.11613635528083208	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4739	0.11538159070772812	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4662	0.11350685289711511	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4600	0.11199732375090724	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4509	0.10978172452018277	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4462	0.10863740403838003	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4401	0.10715222213646583	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4367	0.10632441583048086	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4214	0.10259928745354849	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4148	0.10099236933016592	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4347244293675488E-5
2	0.0	0.0	0.0	2.4347244293675488E-5	2.4347244293675488E-5
3	0.0	0.0	0.0	2.4347244293675488E-5	2.4347244293675488E-5
4	0.0	0.0	0.0	2.4347244293675488E-5	2.4347244293675488E-5
5	0.0	0.0	0.0	2.4347244293675488E-5	2.4347244293675488E-5
6	0.0	0.0	0.0	2.4347244293675488E-5	4.8694488587350975E-5
7	0.0	0.0	0.0	2.4347244293675488E-5	4.8694488587350975E-5
8	0.0	0.0	0.0	2.4347244293675488E-5	4.8694488587350975E-5
9	0.0	0.0	0.0	4.8694488587350975E-5	4.8694488587350975E-5
10-11	0.0	0.0	0.0	1.2173622146837744E-4	7.304173288102646E-5
12-13	0.0	0.0	0.0	1.2173622146837744E-4	3.0434055367094357E-4
14-15	0.0	0.0	0.0	1.2173622146837744E-4	3.895559086988078E-4
16-17	0.0	0.0	0.0	1.4608346576205293E-4	4.6259764157983427E-4
18-19	0.0	0.0	0.0	1.4608346576205293E-4	5.599866187545362E-4
20-21	0.0	0.0	0.0	1.5825708790889067E-4	7.425909509571023E-4
22-23	0.0	0.0	0.0	1.947779543494039E-4	8.03459061691291E-4
24-25	0.0	0.0	0.0	2.6781968723043036E-4	8.03459061691291E-4
26-27	0.0	0.0	0.0	5.843338630482117E-4	9.130216610128308E-4
28-29	0.0	0.0	0.0	0.001326924814005314	9.373689053065062E-4
30-31	0.0	0.0	0.0	0.005563345321104849	9.860633938938571E-4
32-33	0.0	0.0	0.0	0.01592309776806377	0.001046931504628046
34-35	0.0	0.0	0.0	0.030787090409352652	0.0010956259932153969
36-37	0.0	2.4347244293675488E-5	0.0	0.05814121937329706	0.0010956259932153969
38-39	0.0	2.4347244293675488E-5	0.0	0.10351230911456133	0.00124170945897745
40-41	0.0	2.4347244293675488E-5	0.0	0.1552623768607686	0.0012660567032711254
42-43	0.0	2.4347244293675488E-5	0.0	0.2099341139222169	0.0012660567032711254
44-45	0.0	2.4347244293675488E-5	0.0	0.2808941574161341	0.0013512720582989895
46-47	0.0	2.4347244293675488E-5	0.0	0.3694816057786724	0.0014608346576205293
48-49	0.0	2.4347244293675488E-5	0.0	0.47557472278836327	0.0015582236347952312
50-51	0.0	2.4347244293675488E-5	0.0	0.5890937493076253	0.001606918123382582
52-53	0.0	2.4347244293675488E-5	0.0	0.7008476006155957	0.0017408279669977973
54-55	0.0	2.4347244293675488E-5	0.0	0.8306062390787392	0.001850390566319337
56-57	0.0	2.4347244293675488E-5	0.0	0.9872929297306878	0.001959953165640877
58-59	0.0	2.4347244293675488E-5	0.0	1.1774936021528806	0.0020208212763750655
60-61	0.0	2.4347244293675488E-5	0.0	1.3809148282265395	0.0020573421428155786
62-63	0.0	2.4347244293675488E-5	0.0	1.5922245614513488	0.0021912519864307938
64-65	0.0	2.4347244293675488E-5	0.0	1.816109646353842	0.0022034256085776316
66-67	0.0	2.4347244293675488E-5	0.0	2.0771364524263367	0.002227772852871307
68-69	0.0	2.4347244293675488E-5	0.0	2.3710929064060275	0.0022399464750181446
70-71	0.0	2.4347244293675488E-5	0.0	2.6981616126251176	0.0022642937193118203
72-73	0.0	2.4347244293675488E-5	0.0	3.0389378173815462	0.002471245295808062
74-75	0.0	2.4347244293675488E-5	0.0	3.38004270993594	0.0026903704944511416
76-77	0.0	2.4347244293675488E-5	0.0	3.7724715934614013	0.00275123860518533
78-79	0.0	2.4347244293675488E-5	0.0	4.206656000950517	0.002836453960213194
80-81	0.0	2.4347244293675488E-5	0.0	4.678286470163304	0.002885148448800545
82-83	0.0	2.4347244293675488E-5	0.0	5.168311282439964	0.0031407945138841376
84-85	0.0	2.4347244293675488E-5	0.0	5.651457998203661	0.0031407945138841376
86-87	0.0	2.4347244293675488E-5	0.0	6.171137754030017	0.0033477460903803794
88	0.0	2.4347244293675488E-5	0.0	6.587609541295483	0.003554697666876621
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9055	0.0	43.72369	1
GTATCAA	15040	0.0	36.704	1
GGGCACC	3940	0.0	33.30846	94
GTACTAG	3625	0.0	31.131237	1
CAACGCA	17335	0.0	30.6103	5
ATCAACG	17390	0.0	30.469074	3
TATCAAC	19230	0.0	28.630117	2
AACGCAG	19480	0.0	28.226868	6
CTGTGCG	5265	0.0	26.870525	9
TAGTACT	5375	0.0	26.853128	4
TCAACGC	20295	0.0	26.478422	4
TAGGCAT	5025	0.0	25.908436	5
ACGCAGA	20545	0.0	25.802912	7
CGCAGAG	20680	0.0	25.634468	8
TATTCTC	2855	0.0	25.516615	5
TAACGCC	1840	0.0	24.784996	4
ACTGTGC	6195	0.0	24.73102	8
CTAACGC	1840	0.0	24.52948	3
GTACTGT	5975	0.0	24.46179	6
CTAGGCA	5610	0.0	24.387375	4
>>END_MODULE