##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146457_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4034656
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.351545955838613	31.0	30.0	34.0	26.0	34.0
2	30.314995875732652	31.0	30.0	34.0	26.0	34.0
3	30.582358942125424	31.0	30.0	34.0	26.0	34.0
4	34.01920213272209	37.0	35.0	37.0	28.0	37.0
5	34.23939339562035	37.0	35.0	37.0	30.0	37.0
6	34.39875667219213	37.0	35.0	37.0	30.0	37.0
7	34.23703730875693	37.0	35.0	37.0	30.0	37.0
8	34.17381605767629	37.0	35.0	37.0	30.0	37.0
9	35.4958754847997	39.0	35.0	39.0	29.0	39.0
10-11	35.431619820871965	39.0	35.0	39.0	28.5	39.0
12-13	35.420224177724194	39.0	35.0	39.0	28.5	39.0
14-15	36.40406976455985	40.0	36.0	41.0	27.5	41.0
16-17	36.243269934289316	39.5	35.5	41.0	27.0	41.0
18-19	36.20558221072626	39.0	35.0	41.0	27.0	41.0
20-21	36.55555479822815	40.0	36.0	41.0	28.0	41.0
22-23	36.27277282127646	39.5	35.0	41.0	27.0	41.0
24-25	36.394440071222924	39.0	35.0	41.0	28.0	41.0
26-27	36.481583064330636	39.0	35.0	41.0	28.5	41.0
28-29	36.326332901739335	39.0	35.0	41.0	28.0	41.0
30-31	36.27187894085642	39.0	35.0	41.0	28.0	41.0
32-33	36.378518267728396	39.0	35.0	41.0	29.0	41.0
34-35	36.428984528048986	39.0	35.0	41.0	29.0	41.0
36-37	36.35818047437006	39.0	35.0	41.0	29.0	41.0
38-39	36.19124542464091	39.0	35.0	41.0	28.0	41.0
40-41	36.15143124965301	39.0	35.0	41.0	28.0	41.0
42-43	35.98433782210925	39.0	34.5	41.0	28.0	41.0
44-45	35.7836597469524	38.5	34.0	40.5	27.0	41.0
46-47	35.64961548146856	38.0	34.0	40.0	27.0	41.0
48-49	35.51551582092748	38.0	34.0	40.0	27.0	41.0
50-51	35.28090176708993	38.0	33.5	40.0	26.5	41.0
52-53	35.090037911534466	38.0	33.0	40.0	26.0	41.0
54-55	34.89688860710801	37.0	33.0	40.0	26.0	41.0
56-57	34.73797952043495	37.0	33.0	40.0	26.0	41.0
58-59	34.471004715147956	36.5	33.0	40.0	26.0	41.0
60-61	34.216420309439016	36.0	32.5	39.5	26.0	41.0
62-63	33.9390423867611	35.0	32.0	39.0	25.5	41.0
64-65	33.650852018115046	35.0	32.0	39.0	25.0	41.0
66-67	33.36482912049999	35.0	32.0	38.0	25.0	40.0
68-69	33.08142590099379	35.0	31.0	38.0	24.0	40.0
70-71	33.03692570072889	35.0	31.0	37.0	25.0	40.0
72-73	32.83864051854731	35.0	31.0	37.0	24.5	40.0
74-75	32.583115140423374	35.0	31.0	36.0	24.0	39.0
76-77	32.35553948093716	35.0	31.0	36.0	24.0	39.0
78-79	32.14666033485878	35.0	31.0	36.0	24.0	39.0
80-81	31.93565461342925	35.0	31.0	35.0	23.5	37.5
82-83	31.70143167100243	34.5	31.0	35.0	23.0	37.0
84-85	31.51897918930387	34.0	31.0	35.0	23.0	37.0
86-87	31.357516725093788	34.0	30.5	35.0	23.0	36.0
88-89	31.3702795232109	34.5	31.0	35.0	23.0	36.0
90-91	31.28376681927778	34.0	31.0	35.0	23.0	36.0
92-93	31.13691501828161	34.0	31.0	35.0	22.5	36.0
94-95	31.113534214565007	34.0	31.0	35.0	23.0	35.0
96-97	31.025686824353798	34.0	31.0	35.0	21.5	35.0
98-99	30.937097115590525	34.0	30.0	35.0	20.5	35.0
100	30.414957309867308	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8478030890901955
1101	2	1.0842536791190298
1101	3	1.031543008951644
1101	4	1.0882155056457066
1101	5	1.015614573462699
1101	6	0.7527046802115507
1101	7	0.7716117419303501
1101	8	0.8683976155535262
1101	9	1.0585414052907325
1101	10-11	1.126849592559509
1101	12-13	0.9203833532787726
1101	14-15	1.201416080310004
1101	16-17	1.3728711715673683
1101	18-19	1.5093922461929878
1101	20-21	1.1916557347977488
1101	22-23	1.4756787562045943
1101	24-25	1.2841544619997833
1101	26-27	1.2477664156794859
1101	28-29	1.400067141151787
1101	30-31	1.4099003651148578
1101	32-33	1.2425067006316581
1101	34-35	1.1533841776135034
1101	36-37	1.1417205622752462
1101	38-39	1.2946673530333186
1101	40-41	1.145516570463144
1101	42-43	1.1173944551654245
1101	44-45	1.1465860398219974
1101	46-47	1.2140726836069575
1101	48-49	1.176462372363119
1101	50-51	1.189544204086502
1101	52-53	1.1737708489890863
1101	54-55	1.147435517214042
1101	56-57	1.0414512527444089
1101	58-59	1.0067722472648057
1101	60-61	1.0280302150933522
1101	62-63	1.0274215996373428
1101	64-65	1.0215837888116468
1101	66-67	0.9930476449628927
1101	68-69	1.0486490610893355
1101	70-71	0.9406958120409641
1101	72-73	0.9702616509388235
1101	74-75	0.9515128466150244
1101	76-77	0.912373195631595
1101	78-79	0.9324794894116621
1101	80-81	0.9490611446498001
1101	82-83	0.9408986687785514
1101	84-85	0.9629629878312969
1101	86-87	0.932516848529751
1101	88-89	0.9533029414161973
1101	90-91	0.9284671446634292
1101	92-93	0.975258534369587
1101	94-95	0.9507027258566048
1101	96-97	1.0125557461961243
1101	98-99	1.0047563494457954
1101	100	1.1062768567931442
1102	1	0.9387007266260561
1102	2	1.0722531785261964
1102	3	1.0375977113021513
1102	4	1.149680308099775
1102	5	0.9889182464418624
1102	6	0.7523456576345637
1102	7	0.8580443364520107
1102	8	0.9222013037980759
1102	9	1.1457370403611975
1102	10-11	1.1400452801500336
1102	12-13	1.026708005119275
1102	14-15	1.2789869589244063
1102	16-17	1.4412288109299851
1102	18-19	1.4544999617363743
1102	20-21	1.220003519914691
1102	22-23	1.4975726637239077
1102	24-25	1.3680181753721072
1102	26-27	1.2243140532572951
1102	28-29	1.378136779736323
1102	30-31	1.461272331900929
1102	32-33	1.2721498955395916
1102	34-35	1.1956142622513681
1102	36-37	1.1546950479289464
1102	38-39	1.1470186382327157
1102	40-41	1.1284078850529227
1102	42-43	1.198670653702294
1102	44-45	1.2774263054331172
1102	46-47	1.1629853571801902
1102	48-49	1.1276561472171949
1102	50-51	1.25840183888603
1102	52-53	1.132636503899974
1102	54-55	1.2128441787360273
1102	56-57	1.0723864764334223
1102	58-59	0.9685715256556051
1102	60-61	1.108762528617497
1102	62-63	1.0558147106237712
1102	64-65	0.9524413738366135
1102	66-67	0.9733791918773633
1102	68-69	1.016135452333856
1102	70-71	0.9326213121743052
1102	72-73	0.9007074645482191
1102	74-75	0.8803886387087445
1102	76-77	0.8291437266680006
1102	78-79	0.8893034592160447
1102	80-81	0.9598607512019335
1102	82-83	0.8730049395630246
1102	84-85	0.8885652378450857
1102	86-87	0.8979997117794127
1102	88-89	0.8728082772980557
1102	90-91	0.8710587151854838
1102	92-93	0.8818229023537185
1102	94-95	0.9042075480791674
1102	96-97	0.9326342649395976
1102	98-99	0.9212855068810946
1102	100	0.998906608576938
1103	1	0.7262402495505853
1103	2	0.938871356316568
1103	3	0.9011004482234242
1103	4	0.9847688769320015
1103	5	0.9116055513877726
1103	6	0.663389587735054
1103	7	0.7820827498299181
1103	8	0.7748399716818852
1103	9	0.8546398827105222
1103	10-11	0.9611834510000392
1103	12-13	0.9078644247803496
1103	14-15	1.12011392723727
1103	16-17	1.3134166526383098
1103	18-19	1.3157785295557574
1103	20-21	1.0124040367585536
1103	22-23	1.238796794052952
1103	24-25	1.1548650049840958
1103	26-27	1.0349192612333198
1103	28-29	1.2441855959646304
1103	30-31	1.2581884646832293
1103	32-33	1.1037076167490767
1103	34-35	1.1171767671673933
1103	36-37	1.0257133368044293
1103	38-39	1.1678764795915342
1103	40-41	1.0561578621340573
1103	42-43	1.0315407879909202
1103	44-45	1.0450398527226525
1103	46-47	1.0361180190311927
1103	48-49	1.0311848224239384
1103	50-51	1.101244589625523
1103	52-53	1.134730586355019
1103	54-55	1.039676249351487
1103	56-57	1.0040952231769609
1103	58-59	1.0208468291939141
1103	60-61	1.0177299932393424
1103	62-63	0.9529086947905512
1103	64-65	0.937209844532461
1103	66-67	1.045534710825315
1103	68-69	1.0583755371598613
1103	70-71	0.9875115613819361
1103	72-73	0.9487980387451529
1103	74-75	0.9273679614155625
1103	76-77	0.9166705999835258
1103	78-79	0.9114303815749523
1103	80-81	0.9484851053182446
1103	82-83	0.7923970949817445
1103	84-85	0.8896976193743313
1103	86-87	0.9386073590092181
1103	88-89	0.8368433103863282
1103	90-91	0.7739147938650746
1103	92-93	0.9162162903150062
1103	94-95	0.895045028397945
1103	96-97	0.8728851012358056
1103	98-99	0.9282056463631179
1103	100	0.9921113119789986
1104	1	0.5692940477979995
1104	2	0.7818597987283233
1104	3	0.7098467764143201
1104	4	0.7891765116485701
1104	5	0.7332444291866409
1104	6	0.4862399714049417
1104	7	0.5205061737430583
1104	8	0.5147504605503102
1104	9	0.6393024222595258
1104	10-11	0.6644990406717426
1104	12-13	0.6046612029560876
1104	14-15	0.8213597982762764
1104	16-17	0.9332292779847364
1104	18-19	0.8535763853872069
1104	20-21	0.7895504920054037
1104	22-23	1.056928915733117
1104	24-25	0.8355732879938884
1104	26-27	0.7134141457143031
1104	28-29	0.941485737103605
1104	30-31	0.9628965127836224
1104	32-33	0.841079798448142
1104	34-35	0.6542540265780161
1104	36-37	0.6724294169687965
1104	38-39	0.8222447539255526
1104	40-41	0.7282896668020129
1104	42-43	0.6798957354088486
1104	44-45	0.7447292229387017
1104	46-47	0.6952257213055546
1104	48-49	0.7262243873102392
1104	50-51	0.8342618832617035
1104	52-53	0.7965205393276946
1104	54-55	0.7345398330421986
1104	56-57	0.648584271228664
1104	58-59	0.6524177678064049
1104	60-61	0.6887630013510275
1104	62-63	0.5962458431002702
1104	64-65	0.6500301763186584
1104	66-67	0.7317996208000963
1104	68-69	0.6946724835459008
1104	70-71	0.5720242143646246
1104	72-73	0.5565092013034416
1104	74-75	0.618684716796345
1104	76-77	0.6644861069121859
1104	78-79	0.6559077946307852
1104	80-81	0.7004989275125588
1104	82-83	0.6246925895292037
1104	84-85	0.5916518216302862
1104	86-87	0.5733654930802814
1104	88-89	0.5247842598446724
1104	90-91	0.5232452982757891
1104	92-93	0.5623598603892006
1104	94-95	0.51506930069662
1104	96-97	0.5376997765158507
1104	98-99	0.6099765329068667
1104	100	0.5894461497100991
1105	1	0.5625478565328663
1105	2	0.7523091589973454
1105	3	0.7380988003097251
1105	4	0.853905726329856
1105	5	0.7938624039320175
1105	6	0.3870298433522805
1105	7	0.44476639314675026
1105	8	0.4804104889120495
1105	9	0.6069189550898528
1105	10-11	0.6176688177145948
1105	12-13	0.5725580222509947
1105	14-15	0.7393748920205212
1105	16-17	0.8454185730747952
1105	18-19	0.959455706411056
1105	20-21	0.7146700377187614
1105	22-23	0.9214009041466866
1105	24-25	0.8417646352564319
1105	26-27	0.6704396044048693
1105	28-29	0.8921481581794026
1105	30-31	0.8895806765045791
1105	32-33	0.7438875580307496
1105	34-35	0.6647266437539514
1105	36-37	0.6002842842977572
1105	38-39	0.7393985948184536
1105	40-41	0.6403842951830256
1105	42-43	0.6573177316375904
1105	44-45	0.6705078486990033
1105	46-47	0.5738310349204312
1105	48-49	0.6795516337858487
1105	50-51	0.7175726481732809
1105	52-53	0.6937867031771248
1105	54-55	0.6604060530756612
1105	56-57	0.6421587224063714
1105	58-59	0.6280976126772089
1105	60-61	0.6632606720860892
1105	62-63	0.6189240777371268
1105	64-65	0.5723583279139604
1105	66-67	0.6186548675056756
1105	68-69	0.5751450216322382
1105	70-71	0.5147144412164195
1105	72-73	0.49463344833617384
1105	74-75	0.5620985248173795
1105	76-77	0.5564687175414278
1105	78-79	0.5548203657867177
1105	80-81	0.615030377620986
1105	82-83	0.5245024221213512
1105	84-85	0.464167691897579
1105	86-87	0.5460752063941499
1105	88-89	0.5847930798566523
1105	90-91	0.605608744621577
1105	92-93	0.5899047040688501
1105	94-95	0.5218988655876906
1105	96-97	0.6119078170592083
1105	98-99	0.6239260001243032
1105	100	0.6855079791246688
1106	1	0.710739659589084
1106	2	0.7751561373770315
1106	3	0.7458254693909758
1106	4	0.8757169699634844
1106	5	0.7629323107348611
1106	6	0.48991768791857027
1106	7	0.5008876484069305
1106	8	0.6074274480199975
1106	9	0.7691854466841903
1106	10-11	0.6930685247490516
1106	12-13	0.6004718825128847
1106	14-15	0.7898759981057424
1106	16-17	0.8465858217680733
1106	18-19	0.8596967853566753
1106	20-21	0.7396505477911646
1106	22-23	1.068595967290861
1106	24-25	0.9001264957324153
1106	26-27	0.7598628972724484
1106	28-29	1.04914854972548
1106	30-31	0.957469059808723
1106	32-33	0.8557109364972248
1106	34-35	0.8086927998329401
1106	36-37	0.7229745643585588
1106	38-39	0.8659124906100786
1106	40-41	0.8061863600019308
1106	42-43	0.7781859893134566
1106	44-45	0.8324525954657531
1106	46-47	0.8177353834385812
1106	48-49	0.8268282315829438
1106	50-51	0.8134710057667931
1106	52-53	0.8222684650286354
1106	54-55	0.796628863665525
1106	56-57	0.7013201267676195
1106	58-59	0.7313816217895237
1106	60-61	0.6332381199905441
1106	62-63	0.6345373396554876
1106	64-65	0.7245490986306251
1106	66-67	0.7445859877498648
1106	68-69	0.6807093298054667
1106	70-71	0.7214790812801226
1106	72-73	0.6668005585253951
1106	74-75	0.6409187927842908
1106	76-77	0.6770759195327756
1106	78-79	0.7196355767078231
1106	80-81	0.6494028009536983
1106	82-83	0.7317544976101615
1106	84-85	0.688993231207121
1106	86-87	0.7104449475213741
1106	88-89	0.713631437232479
1106	90-91	0.7492743009590299
1106	92-93	0.7870530732709398
1106	94-95	0.78438884601238
1106	96-97	0.782116622661924
1106	98-99	0.7280257302361157
1106	100	0.6864665730019119
1107	1	0.5346253412067128
1107	2	0.7422659834351215
1107	3	0.7001883735361893
1107	4	0.8287102796090693
1107	5	0.7170706861403744
1107	6	0.41338462687344446
1107	7	0.43598711015894764
1107	8	0.4692119833867707
1107	9	0.6247054571889024
1107	10-11	0.5939035095169842
1107	12-13	0.5343870754504465
1107	14-15	0.7621699029788473
1107	16-17	0.8729637652858031
1107	18-19	0.8939683729310559
1107	20-21	0.7235570895105425
1107	22-23	0.9367195346550545
1107	24-25	0.7228191743605166
1107	26-27	0.6718566262058445
1107	28-29	0.836527548217795
1107	30-31	0.8187490158368291
1107	32-33	0.7592262227257507
1107	34-35	0.6561983929047628
1107	36-37	0.6041840710281718
1107	38-39	0.6776363726415937
1107	40-41	0.4760455632033995
1107	42-43	0.6485776098146232
1107	44-45	0.6590242907181789
1107	46-47	0.6403184523172101
1107	48-49	0.5991071923654303
1107	50-51	0.7036020160228702
1107	52-53	0.7436682368734893
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2210	2	0.019861283428600274
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2210	98-99	0.20965763634661272
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2211	30-31	-0.06662213439047804
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2211	86-87	-0.25286652271634935
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2212	4	-0.38715081422781594
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2212	84-85	-0.101221468557668
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2212	94-95	-0.28198231830665677
2212	96-97	-0.28316324716071506
2212	98-99	-0.34242056432400503
2212	100	-0.3300477012656522
2213	1	-0.5837360916544796
2213	2	-0.44496324888704564
2213	3	-0.5607467288593213
2213	4	-0.6080100467181424
2213	5	-2.314573239869123
2213	6	-1.0938505954337998
2213	7	-0.7119056213078565
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2213	10-11	-0.5699557811029763
2213	12-13	-0.7271729048767028
2213	14-15	-0.6428035771464664
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2213	22-23	-0.7255226467152696
2213	24-25	-0.565875102253564
2213	26-27	-3.1824398623708063
2213	28-29	-1.0891262059978786
2213	30-31	-1.4787771277965902
2213	32-33	-0.9584905398591061
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2213	36-37	-0.873877907392199
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2213	48-49	-1.0159083632971786
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2213	54-55	-0.6439372034801778
2213	56-57	-0.7135360424097499
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2213	62-63	-0.667881061866261
2213	64-65	-0.5955813294028118
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2213	70-71	-0.6378912719958194
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2213	80-81	-0.726213048368006
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2213	84-85	-0.6132419711571657
2213	86-87	-0.5413341042320923
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2213	100	-0.5171846690750606
2214	1	-1.0781116242388151
2214	2	-1.1718616522702412
2214	3	-1.3744553122443897
2214	4	-1.117264758164616
2214	5	-1.3620885486528778
2214	6	-1.1319214406761446
2214	7	-1.3260184554782128
2214	8	-1.203843156772173
2214	9	-4.715050566739421
2214	10-11	-1.9822129897408445
2214	12-13	-1.6454176284034858
2214	14-15	-4.121808843987893
2214	16-17	-3.620629248512998
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2214	20-21	-1.881127845246013
2214	22-23	-3.0062359361829962
2214	24-25	-2.39687073591751
2214	26-27	-1.8099215102479747
2214	28-29	-1.3474594758882645
2214	30-31	-1.3453104258479556
2214	32-33	-1.4521317491386299
2214	34-35	-1.3355675532443172
2214	36-37	-1.3582764786108328
2214	38-39	-1.3918767061623143
2214	40-41	-1.5973940944186467
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2214	44-45	-1.9668063331453283
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2214	52-53	-1.4016373133548825
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2214	58-59	-1.4618167584433337
2214	60-61	-1.5565245683863864
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2214	66-67	-1.2521080768898045
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2214	70-71	-1.2795142380052411
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2214	76-77	-1.3023865459421629
2214	78-79	-1.3192038101308654
2214	80-81	-1.3644207073700692
2214	82-83	-1.433983886645457
2214	84-85	-1.2975298594212887
2214	86-87	-1.17055470413478
2214	88-89	-1.0985725260194705
2214	90-91	-1.1134387265296795
2214	92-93	-1.2053353916621283
2214	94-95	-1.353527873794171
2214	96-97	-1.2850612338369096
2214	98-99	-1.3886851615050908
2214	100	-1.3276946511887608
2215	1	-4.436899551869068
2215	2	-2.5163234555272247
2215	3	-4.22083511717841
2215	4	-2.4068595635687444
2215	5	-1.909267613694702
2215	6	-1.6505514888190262
2215	7	-1.558200312385054
2215	8	-1.375200655568122
2215	9	-2.1187334492489143
2215	10-11	-1.7912573660036628
2215	12-13	-1.6563487681559863
2215	14-15	-4.018040497788959
2215	16-17	-3.7719089972259567
2215	18-19	-2.681803484801499
2215	20-21	-2.1387109152230153
2215	22-23	-3.5543243625113377
2215	24-25	-5.673697565452567
2215	26-27	-3.9258705236930567
2215	28-29	-2.128989648177992
2215	30-31	-4.440039269555321
2215	32-33	-4.406334954698568
2215	34-35	-3.31119601069571
2215	36-37	-2.3650144865431812
2215	38-39	-2.172600211747003
2215	40-41	-2.4066833105281873
2215	42-43	-2.2140409386250752
2215	44-45	-3.687946143221758
2215	46-47	-3.0749241421907527
2215	48-49	-2.225205930194413
2215	50-51	-5.464813828329014
2215	52-53	-5.737948252284998
2215	54-55	-3.491068034869471
2215	56-57	-2.3134751130331423
2215	58-59	-2.064887426207555
2215	60-61	-3.6186650366138124
2215	62-63	-3.220816899858498
2215	64-65	-2.2097227235646812
2215	66-67	-2.044676228137895
2215	68-69	-2.1354862031692186
2215	70-71	-1.904492238876383
2215	72-73	-1.7791563737086022
2215	74-75	-1.9244374529156403
2215	76-77	-2.061989160893127
2215	78-79	-1.9650751694333408
2215	80-81	-1.973334067451578
2215	82-83	-1.8970210520294017
2215	84-85	-1.7344728919431596
2215	86-87	-1.764952246526036
2215	88-89	-1.7128664144254593
2215	90-91	-2.0457509855994047
2215	92-93	-4.254347416829212
2215	94-95	-2.432438758613909
2215	96-97	-2.0145845060945966
2215	98-99	-3.4741475845369223
2215	100	-3.503141359186543
2216	1	-1.371070743797695
2216	2	-1.4044071466522965
2216	3	-1.632833777362535
2216	4	-1.780194385586384
2216	5	-1.821364188705779
2216	6	-1.5241408081857912
2216	7	-1.8230643176901822
2216	8	-1.6914931549675742
2216	9	-1.9966584939940262
2216	10-11	-2.089747163441089
2216	12-13	-2.2170452694095317
2216	14-15	-2.6144933690888124
2216	16-17	-2.44719311600943
2216	18-19	-4.455154276341709
2216	20-21	-2.622871381857877
2216	22-23	-2.035424229544766
2216	24-25	-2.2949236323147915
2216	26-27	-2.2725419270825213
2216	28-29	-2.2391026190166983
2216	30-31	-2.363904909029813
2216	32-33	-2.2749304148881038
2216	34-35	-2.5252857309922376
2216	36-37	-2.5476857275307196
2216	38-39	-2.624075261059673
2216	40-41	-2.6191230949396314
2216	42-43	-2.6617525263407273
2216	44-45	-2.519459424654144
2216	46-47	-2.7359411165752334
2216	48-49	-2.696505044088916
2216	50-51	-2.5776820154837026
2216	52-53	-2.5854971674063094
2216	54-55	-2.5075533188867567
2216	56-57	-2.5365694787013737
2216	58-59	-2.5311372612681
2216	60-61	-2.5364914898572835
2216	62-63	-2.4679496600483724
2216	64-65	-2.59612345708004
2216	66-67	-2.5157089352088065
2216	68-69	-2.5276769036290183
2216	70-71	-2.5999201510304744
2216	72-73	-2.5425532129611454
2216	74-75	-2.5215967725869106
2216	76-77	-2.350057423835377
2216	78-79	-2.5522311395649204
2216	80-81	-2.5627016811244694
2216	82-83	-2.6900224087621467
2216	84-85	-2.4941157430442793
2216	86-87	-2.488998016801748
2216	88-89	-2.345506697546149
2216	90-91	-2.410650719327915
2216	92-93	-2.2666644524527513
2216	94-95	-2.1489092234555827
2216	96-97	-2.2679347562950625
2216	98-99	-2.232571812197353
2216	100	-2.3552449039468826
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16548.0
1	2783.0
2	481.0
3	403.0
4	404.0
5	235.0
6	237.0
7	255.0
8	463.0
9	817.0
10	1511.0
11	2776.0
12	4880.0
13	7437.0
14	9976.0
15	12827.0
16	15715.0
17	18908.0
18	21776.0
19	24656.0
20	27922.0
21	31388.0
22	36641.0
23	42257.0
24	46876.0
25	53190.0
26	59688.0
27	67181.0
28	78496.0
29	92626.0
30	108824.0
31	129691.0
32	158414.0
33	199633.0
34	273046.0
35	362670.0
36	529904.0
37	779666.0
38	608194.0
39	205086.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.604133914642134	21.38567849511248	12.379889772957124	23.630297817288263
2	15.285395397842674	19.93077294991483	41.11525609228414	23.668575559958352
3	18.514001574962382	27.388555970686372	28.851572496833388	25.245869957517858
4	11.911409902016715	16.852482725493658	40.67988432947225	30.556223043017372
5	12.831364739560644	39.145360632273324	34.96127948223287	13.061995145933164
6	29.10788603934676	38.83050606838735	18.681965694246923	13.379642198018962
7	26.682777038908267	31.994790741609375	23.999007570513186	17.323424648969173
8	26.150844887515657	35.05717075095604	20.161636070721883	18.63034829080642
9	26.451563555766388	14.88158474334724	21.780504236519576	36.88634746436679
10-11	25.67295623812809	26.071097250016255	27.5303745112008	20.725572000654847
12-13	25.776989545119573	24.039210629429526	27.55957064161432	22.62422918383658
14-15	23.274752078363157	25.06983940580784	25.0657031737732	26.589705342055797
16-17	22.014728490260598	27.63542407857853	26.46487203865634	23.884975392504533
18-19	21.34113925509429	28.42842699421969	28.104694703780098	22.125739046905924
20-21	23.42774459386239	27.42227320348063	26.84762521648658	22.3023569861704
22-23	23.883115505093176	27.135009006711197	26.523631159235467	22.458244328960163
24-25	23.157482948914332	27.238393768730656	27.40736910889584	22.196754173459173
26-27	23.032745199126648	27.42257064716519	26.322025244685015	23.22265890902315
28-29	22.046511303451805	28.01192959089879	27.01069250996136	22.930866595688045
30-31	23.034471246555615	27.50174399194774	26.901225278914144	22.562559482582504
32-33	23.41492643665131	27.843626264180276	25.857773996301436	22.88367330286698
34-35	23.333493635824965	27.59901835073155	26.954764298027083	22.1127237154164
36-37	22.946309144664223	27.172931592527398	26.62484040383096	23.255918858977424
38-39	23.219038916154453	27.19065891681981	27.2440368197884	22.34626534723734
40-41	22.77576346317127	26.280338597576826	27.919484512677222	23.02441342657468
42-43	22.690861932677365	26.614173758147686	27.164114497721787	23.530849811453162
44-45	22.910701555069313	27.622278323399733	26.951805699017306	22.515214422513644
46-47	22.788405738587926	27.286853756825163	26.479641306478424	23.445099198108487
48-49	23.552223165126986	26.836779032898626	26.832842419352993	22.778155382621396
50-51	23.170070592011268	26.66479690515014	26.74680559219006	23.41832691064853
52-53	22.527073851276835	27.38378464309261	26.60092163969539	23.48821986593516
54-55	22.52095100407451	27.233545429840877	26.567676113469286	23.677827452615325
56-57	23.73965409069866	26.595209773604672	26.283866984334065	23.381269151362602
58-59	22.574976651559535	27.02675427273969	27.306362437567216	23.091906638133562
60-61	22.558480774343433	27.79806115144392	26.427307630687398	23.216150443525247
62-63	23.051262358707575	26.944346340925613	25.993533265190315	24.010858035176497
64-65	22.129534514733027	27.14484968581629	26.579437366027292	24.14617843342339
66-67	22.89632351612865	26.673660119441028	26.51889883019552	23.911117534234798
68-69	22.253806157766682	26.873707526332353	26.74900698769132	24.123479328209648
70-71	22.259187622817688	26.343201276232463	27.04170780316875	24.355903297781094
72-73	22.945907239721468	26.553009130796333	27.31780459183788	23.18327903764432
74-75	23.068745875159028	26.882581468607224	26.83641938199619	23.212253274237558
76-77	22.675449699170713	26.77611115485159	26.55964562240319	23.98879352357451
78-79	22.544259493861755	26.75949448483826	26.56060621543288	24.135639805867104
80-81	22.0585400992616	27.104095110935805	27.349987896598204	23.48737689320439
82-83	23.024034689905893	26.023160241357267	28.37064160155021	22.58216346718663
84-85	23.266809909550158	26.599094567091058	26.858950198698107	23.27514532466068
86-87	21.730149039594128	27.34401332739283	27.461040433965106	23.464797199047936
88-89	22.97042668008298	25.922019564341596	27.218268406025565	23.889285349549862
90-91	22.645110371952708	25.979354601699576	27.432102821285508	23.943432205062205
92-93	22.881158856639956	26.29901763013356	27.2604881427491	23.55933537047738
94-95	21.553289763362383	26.2662193890631	28.023377823020112	24.15711302455441
96-97	22.004069312375417	26.164934325047067	27.484823399385633	24.346172963191883
98-99	21.277104361116432	26.951741398313096	27.38991152529135	24.381242715279125
100	22.185728571596808	26.0953962624967	26.489086691825236	25.229788474081253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14137.0
1	8390.5
2	2461.5
3	1806.5
4	1055.0
5	627.5
6	445.5
7	431.5
8	452.0
9	438.5
10	400.5
11	391.0
12	379.5
13	354.0
14	388.0
15	487.0
16	563.0
17	634.0
18	764.0
19	903.0
20	1123.5
21	1448.5
22	1950.5
23	2740.0
24	3613.5
25	4714.0
26	6679.5
27	9029.0
28	12879.0
29	21164.5
30	32793.5
31	43865.5
32	58386.0
33	72847.5
34	84196.0
35	96440.5
36	107659.0
37	127096.5
38	135252.5
39	125869.5
40	115468.0
41	104981.5
42	103293.0
43	110360.0
44	121965.5
45	144824.0
46	166401.5
47	181261.0
48	190742.0
49	186765.5
50	177843.0
51	182525.0
52	184984.5
53	176149.5
54	166614.0
55	143815.0
56	125023.5
57	102969.0
58	80136.0
59	75853.0
60	63490.5
61	44113.5
62	32491.5
63	26994.0
64	19881.5
65	9589.5
66	5016.0
67	3291.0
68	2300.5
69	1714.0
70	1308.5
71	1011.5
72	771.0
73	648.0
74	529.5
75	393.0
76	278.5
77	180.0
78	121.5
79	84.0
80	56.5
81	45.5
82	31.5
83	23.0
84	23.0
85	20.0
86	15.0
87	11.5
88	8.0
89	7.5
90	6.5
91	4.5
92	4.0
93	4.0
94	3.5
95	2.0
96	3.0
97	3.0
98	1.5
99	1.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9912121380360557
2	0.4465808237430899
3	0.4789008034390045
4	0.4651449838598384
5	0.4314122443152527
6	0.48544411221179695
7	0.5022733040933354
8	0.5069329330678998
9	0.5200939063949939
10-11	0.5330070271170578
12-13	0.5318545125036682
14-15	0.5289546370248169
16-17	0.5341099712094414
18-19	0.5319784388061832
20-21	0.5308135315625421
22-23	0.4925326967156555
24-25	0.529128133848338
26-27	0.5072179635636842
28-29	0.5148270385381059
30-31	0.5174170982606695
32-33	0.5189661770421071
34-35	0.5306524273692727
36-37	0.5498610042590991
38-39	0.5393148759150719
40-41	0.5449535226795047
42-43	0.5418057945956235
44-45	0.5517818619480818
46-47	0.5503691020994107
48-49	0.5218288746302039
50-51	0.5370098466882927
52-53	0.5297601579911646
54-55	0.5398601516461379
56-57	0.5262902215207443
58-59	0.5338745112346629
60-61	0.5265132888652713
62-63	0.5214447030924074
64-65	0.5323502177137283
66-67	0.5298716916634281
68-69	0.5513109419985247
70-71	0.535212915301825
72-73	0.5391537717218022
74-75	0.5359192952261606
76-77	0.5225724324452939
78-79	0.5540249280236035
80-81	0.5284589318147569
82-83	0.5271700982686008
84-85	0.5368115646042686
86-87	0.839563521648438
88-89	1.102659061887804
90-91	1.0925714608630823
92-93	1.0930795587033937
94-95	1.0978135434594671
96-97	1.0919270440900042
98-99	1.0869452067289007
100	1.083190239762696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4034656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.399483505832137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40927943367818	22.463933504029406
2	11.809440833570475	6.943829219993114
3	4.454355492441999	3.9286725248748393
4	2.2239833217340674	2.615358439382659
5	1.240536050971768	1.8235559584467316
6	0.7691988339034952	1.3568429058030673
7	0.48730974653923675	1.002865839892807
8	0.35809507338388297	0.8422248162775373
9	0.2605273200090014	0.6893431782680959
>10	1.4683049075792582	8.309955260731062
>50	0.20074125070273885	4.188912602249046
>100	0.23790948911002274	15.526867189278375
>500	0.04677505429018949	9.74853890537294
>1k	0.031857604543581775	16.51609731265844
>5k	0.0011799112793919177	2.1700311034272812
>10k+	5.056762625965361E-4	1.8729712393145883
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	16136	0.3999349634764401	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13435	0.3329899748578317	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12634	0.31313698119492717	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12361	0.3063706050776076	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10890	0.2699114868776917	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10135	0.25119861519792513	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9885	0.2450023000721747	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8491	0.2104516469309899	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6788	0.1682423482943775	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6347	0.15731204841255364	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6333	0.1569650547655116	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6284	0.1557505770008645	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6058	0.15014910812718604	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5923	0.1468030979592808	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5560	0.13780604839669108	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5352	0.13265071421206664	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5248	0.13007304711975445	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5167	0.12806544101901127	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5093	0.12623133174178913	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5051	0.12519035080066307	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4979	0.12340581204444692	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4906	0.12159648802772777	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4834	0.11981194927151162	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4733	0.11730863796070844	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4698	0.11644115384310334	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4674	0.11584630759103129	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4667	0.11567281076751029	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4627	0.1146814003473902	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4551	0.11279772054916205	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4514	0.111880665910551	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4274	0.1059322033898305	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4272	0.10588263286882452	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4254	0.10543649817977047	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4140	0.10261097848242824	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	4120	0.1021152732723682	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4119	0.1020904880118652	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4083	0.10119821863375712	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4065	0.1007520839447031	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.957052100600398E-5
2	0.0	0.0	0.0	0.0	4.957052100600398E-5
3	0.0	0.0	0.0	0.0	4.957052100600398E-5
4	0.0	0.0	0.0	0.0	4.957052100600398E-5
5	0.0	0.0	0.0	0.0	4.957052100600398E-5
6	0.0	0.0	0.0	0.0	7.435578150900598E-5
7	0.0	0.0	0.0	0.0	7.435578150900598E-5
8	0.0	0.0	0.0	0.0	7.435578150900598E-5
9	0.0	0.0	0.0	0.0	1.2392630251500996E-4
10-11	0.0	0.0	0.0	3.717789075450298E-5	1.2392630251500996E-4
12-13	0.0	0.0	0.0	4.957052100600398E-5	3.9656416804803184E-4
14-15	0.0	0.0	0.0	4.957052100600398E-5	4.957052100600398E-4
16-17	0.0	0.0	0.0	7.435578150900598E-5	6.196315125750497E-4
18-19	0.0	0.0	0.0	7.435578150900598E-5	6.939872940840558E-4
20-21	0.0	0.0	0.0	7.435578150900598E-5	7.683430755930616E-4
22-23	0.0	0.0	0.0	1.2392630251500996E-4	8.426988571020677E-4
24-25	0.0	0.0	0.0	3.0981575628752486E-4	9.170546386110737E-4
26-27	0.0	0.0	0.0	5.204904705630418E-4	9.170546386110737E-4
28-29	0.0	0.0	0.0	0.0015366861511861233	9.666251596170776E-4
30-31	0.0	0.0	0.0	0.006159137234995995	9.790177898685785E-4
32-33	0.0	0.0	0.0	0.01808084753693995	0.0010161956806230817
34-35	0.0	0.0	0.0	0.03685568236796396	0.0010161956806230817
36-37	0.0	0.0	0.0	0.06860560107230951	0.0010161956806230817
38-39	0.0	0.0	0.0	0.11914274723793056	0.0010409809411260837
40-41	0.0	0.0	0.0	0.17860258718463234	0.0010409809411260837
42-43	0.0	0.0	0.0	0.24111101417320335	0.0010781588318805866
44-45	0.0	0.0	0.0	0.31618556823679644	0.0011525146133895925
46-47	0.0	0.0	0.0	0.4155868554840859	0.0012516556554016006
48-49	0.0	0.0	0.0	0.5351881300413219	0.0012888335461561035
50-51	0.0	0.0	0.0	0.6641458404384414	0.0013384040671621075
52-53	0.0	0.0	0.0	0.7924343488019796	0.0013507966974136087
54-55	0.0	0.0	0.0	0.9320497212153899	0.0013631893276651096
56-57	0.0	0.0	0.0	1.10658752567753	0.0013879745881681116
58-59	0.0	0.0	0.0	1.3180057977681368	0.0013879745881681116
60-61	0.0	0.0	0.0	1.5432170673286645	0.0014499377394256163
62-63	0.0	0.0	0.0	1.7830268553254602	0.0015614714116891253
64-65	0.0	0.0	0.0	2.031251239263025	0.0015862566721921273
66-67	0.0	0.0	0.0	2.31423695105605	0.0015986493024436285
68-69	0.0	0.0	0.0	2.642592082199821	0.0016110419326951294
70-71	0.0	0.0	0.0	3.0070469452662136	0.0016482198234496325
72-73	0.0	0.0	0.0	3.384489284836179	0.0019456429494856561
74-75	0.0	0.0	0.0	3.754508438885496	0.002019998730994662
76-77	0.0	0.0	0.0	4.178596638722112	0.0020819618822521674
78-79	0.0	0.0	0.0	4.636281259170547	0.002106747142755169
80-81	0.0	0.0	0.0	5.148233207490303	0.002131532403258171
82-83	0.0	0.0	0.0	5.678179254935241	0.002181102924264175
84-85	0.0	0.0	0.0	6.188383842389538	0.002181102924264175
86-87	0.0	0.0	0.0	6.735865461640348	0.002280243966276183
88	0.0	0.0	2.478526050300199E-5	7.175432056661088	0.0023793850082881912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4310	0.0	38.4443	94
GTACTAG	4670	0.0	35.55194	1
TAGGCAT	5500	0.0	32.634495	5
GTATCAA	9915	0.0	32.586323	1
GCATCGT	4830	0.0	32.564835	8
CAACGCA	9235	0.0	31.087124	5
GGTATCA	6500	0.0	30.259394	1
CTAGGCA	6235	0.0	29.389954	4
CTGTGCG	5925	0.0	28.767488	9
ATCAACG	9955	0.0	28.744333	3
TAGTACT	6025	0.0	28.698654	4
CAGTACG	725	0.0	28.496948	9
AGGGCTA	5410	0.0	27.445177	5
CTATTGA	5275	0.0	27.416498	9
TATCAAC	11675	0.0	27.408337	2
ACTGTGC	6415	0.0	27.226854	8
TATTCTC	2360	0.0	26.878065	5
ATTTAGG	5760	0.0	26.285732	1
AACGCAG	11685	0.0	26.236664	6
GCTATTG	5445	0.0	26.213614	8
>>END_MODULE