##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4034656
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.824841572614865	31.0	31.0	34.0	30.0	34.0
2	31.77078442375261	31.0	31.0	34.0	30.0	34.0
3	31.956499141438577	31.0	31.0	34.0	30.0	34.0
4	35.70680870934226	37.0	35.0	37.0	33.0	37.0
5	35.62247809974382	37.0	35.0	37.0	33.0	37.0
6	35.66063129049912	37.0	35.0	37.0	33.0	37.0
7	35.579525738997326	37.0	35.0	37.0	33.0	37.0
8	35.56523331852827	37.0	35.0	37.0	33.0	37.0
9	37.14381498695304	39.0	37.0	39.0	33.0	39.0
10-11	37.08950936585424	39.0	37.0	39.0	33.0	39.0
12-13	37.022482709802276	39.0	37.0	39.0	33.0	39.0
14-15	38.19918067364355	40.0	37.0	41.0	33.0	41.0
16-17	38.201459926199405	40.0	37.0	41.0	33.0	41.0
18-19	38.26808022790543	40.0	37.5	41.0	33.0	41.0
20-21	38.287178014680805	40.0	38.0	41.0	33.0	41.0
22-23	38.248953318448955	40.0	37.5	41.0	33.0	41.0
24-25	38.176000630537025	40.0	37.0	41.0	33.0	41.0
26-27	38.02707095722659	40.0	37.0	41.0	33.0	41.0
28-29	37.95778066333288	40.0	37.0	41.0	33.0	41.0
30-31	37.8195364115305	40.0	37.0	41.0	33.0	41.0
32-33	37.687863104066366	40.0	36.0	41.0	32.0	41.0
34-35	37.585268607782176	40.0	36.0	41.0	32.0	41.0
36-37	37.500721622859544	40.0	36.0	41.0	32.0	41.0
38-39	37.37762240448752	40.0	36.0	41.0	31.0	41.0
40-41	37.243169801836885	39.0	35.5	41.0	31.0	41.0
42-43	37.10424098113941	39.0	35.0	41.0	31.0	41.0
44-45	36.8836726105026	39.0	35.0	41.0	30.5	41.0
46-47	36.61695532407224	39.0	35.0	41.0	30.0	41.0
48-49	36.323526962397786	39.0	35.0	41.0	29.5	41.0
50-51	36.10854791585702	38.5	35.0	41.0	29.0	41.0
52-53	35.91130458210068	38.0	35.0	40.0	28.5	41.0
54-55	35.708567223574946	38.0	34.5	40.0	28.0	41.0
56-57	35.440991499647055	37.5	34.0	40.0	28.0	41.0
58-59	35.149624156309734	37.0	34.0	40.0	27.5	41.0
60-61	34.887401181166375	36.5	34.0	40.0	27.0	41.0
62-63	34.612045363966594	36.0	34.0	39.0	26.5	41.0
64-65	34.336310827986324	35.5	33.0	39.0	26.0	41.0
66-67	34.11841777836821	35.0	33.0	39.0	26.0	41.0
68-69	33.93032379960027	35.0	33.0	38.5	26.0	41.0
70-71	33.734597447713014	35.0	33.0	37.5	26.0	40.0
72-73	33.54229084214367	35.0	33.0	37.0	26.0	40.0
74-75	33.362740714450005	35.0	33.0	36.5	26.0	39.5
76-77	33.211219494301375	35.0	33.0	36.0	26.0	39.0
78-79	33.100740310945966	35.0	33.0	36.0	26.0	39.0
80-81	32.96414155258837	35.0	33.0	36.0	26.0	38.0
82-83	32.77469107651309	35.0	33.0	35.0	26.0	37.0
84-85	32.63219999424982	35.0	33.0	35.0	26.0	37.0
86-87	32.50279230744827	35.0	33.0	35.0	26.0	36.5
88-89	32.39379776119699	35.0	33.0	35.0	26.0	36.0
90-91	32.29936021807063	35.0	33.0	35.0	25.5	36.0
92-93	32.21717142675857	35.0	33.0	35.0	25.0	36.0
94-95	32.1804437602611	35.0	33.0	35.0	25.0	35.0
96-97	32.12960175539129	35.0	33.0	35.0	25.0	35.0
98-99	32.09184550058295	35.0	32.0	35.0	25.0	35.0
100	31.588135890643464	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4240248783834275
1101	2	0.5330161525310011
1101	3	0.4307517595707395
1101	4	0.2931826346910711
1101	5	0.31990853519742046
1101	6	0.3429515807613228
1101	7	0.3493235480774928
1101	8	0.4023764823650353
1101	9	0.3813462019124927
1101	10-11	0.4442128478642431
1101	12-13	0.47761774698487613
1101	14-15	0.6243726370105804
1101	16-17	0.6289509213140363
1101	18-19	0.6061794977527484
1101	20-21	0.5761640680739646
1101	22-23	0.6373225328524299
1101	24-25	0.616636305548333
1101	26-27	0.665424121070167
1101	28-29	0.6860407673261335
1101	30-31	0.726955987551591
1101	32-33	0.7627130266922109
1101	34-35	0.7016373941989329
1101	36-37	0.6766634576621087
1101	38-39	0.6956088127642346
1101	40-41	0.6430301014210968
1101	42-43	0.6711120558603625
1101	44-45	0.6072897019158887
1101	46-47	0.6820123071294333
1101	48-49	0.7352710741215489
1101	50-51	0.730975083289934
1101	52-53	0.729646496147943
1101	54-55	0.6946722484584171
1101	56-57	0.7138138958515938
1101	58-59	0.7285875631118728
1101	60-61	0.7340630270349706
1101	62-63	0.683289221829888
1101	64-65	0.6959267161673566
1101	66-67	0.6414161472193314
1101	68-69	0.6302475211584309
1101	70-71	0.5774838016214829
1101	72-73	0.5644414761271861
1101	74-75	0.5381074329789755
1101	76-77	0.533162984863992
1101	78-79	0.4785586146379117
1101	80-81	0.5264635693809439
1101	82-83	0.5269315748598657
1101	84-85	0.5430818281682406
1101	86-87	0.5164506468638166
1101	88-89	0.4916131027441537
1101	90-91	0.5295781228011833
1101	92-93	0.529675336025349
1101	94-95	0.5849403034794918
1101	96-97	0.49097042364040533
1101	98-99	0.528106207282633
1101	100	0.5711400031329852
1102	1	0.45981063105686104
1102	2	0.5473581931341904
1102	3	0.4887785579635846
1102	4	0.32598567568830816
1102	5	0.3511245606555562
1102	6	0.41080890692295213
1102	7	0.41775867103528697
1102	8	0.4107232043704485
1102	9	0.4950179916226247
1102	10-11	0.4844017812392565
1102	12-13	0.5204116086673594
1102	14-15	0.6597591000418248
1102	16-17	0.6047608759172434
1102	18-19	0.6412682412548278
1102	20-21	0.6876119286179261
1102	22-23	0.6675441855058395
1102	24-25	0.6815117080886992
1102	26-27	0.7327104632976642
1102	28-29	0.7135471894019005
1102	30-31	0.7461945948002295
1102	32-33	0.7181714371055747
1102	34-35	0.7514769754478934
1102	36-37	0.7650405014794117
1102	38-39	0.781758978564639
1102	40-41	0.7305675235444298
1102	42-43	0.7416745585438775
1102	44-45	0.7055171019810587
1102	46-47	0.6927849164446584
1102	48-49	0.6443031344589016
1102	50-51	0.658985578351384
1102	52-53	0.6632532362445502
1102	54-55	0.601396604305485
1102	56-57	0.6747308958972766
1102	58-59	0.6232628258817954
1102	60-61	0.6077380747167425
1102	62-63	0.5313506791331122
1102	64-65	0.5350768990761807
1102	66-67	0.5402536238277662
1102	68-69	0.5128446980558365
1102	70-71	0.5628512610892074
1102	72-73	0.5704955429495158
1102	74-75	0.4834115394391958
1102	76-77	0.45239560289511616
1102	78-79	0.4969467453713463
1102	80-81	0.4322074704903187
1102	82-83	0.5043015096335779
1102	84-85	0.465226703222001
1102	86-87	0.48972742429058513
1102	88-89	0.5434667363345511
1102	90-91	0.5511833607125496
1102	92-93	0.5443753569393053
1102	94-95	0.4848373453729167
1102	96-97	0.5394836575867927
1102	98-99	0.4559284970301434
1102	100	0.5268691612597358
1103	1	0.29333376879844764
1103	2	-0.12807962683059415
1103	3	0.21951839167017795
1103	4	0.2029520705825476
1103	5	0.2461652758903341
1103	6	0.26740041617577504
1103	7	0.31366571787492603
1103	8	0.3206085212121508
1103	9	0.3733125308843199
1103	10-11	0.4306773522352927
1103	12-13	0.43423651539877284
1103	14-15	0.548952025281082
1103	16-17	0.4885232261086827
1103	18-19	0.47150122783423143
1103	20-21	0.4928866063402708
1103	22-23	0.46515686047089133
1103	24-25	0.47383386555144824
1103	26-27	0.5208663608297854
1103	28-29	0.5614103392826593
1103	30-31	0.5772653564340402
1103	32-33	0.5600338883518248
1103	34-35	0.5482076262667945
1103	36-37	0.6051260662555293
1103	38-39	0.5960219435549803
1103	40-41	0.5426093417000857
1103	42-43	0.5572033756163535
1103	44-45	0.6337949684019648
1103	46-47	0.6876752054325621
1103	48-49	0.6791062462782165
1103	50-51	0.6440883841286862
1103	52-53	0.5821529687584004
1103	54-55	0.6403095087381203
1103	56-57	0.6439731662357744
1103	58-59	0.5916782090772585
1103	60-61	0.6392100410314328
1103	62-63	0.650802510122034
1103	64-65	0.6273197104005064
1103	66-67	0.6658756328972046
1103	68-69	0.5508815582983786
1103	70-71	0.5670240692654716
1103	72-73	0.5455838854991697
1103	74-75	0.572419117038045
1103	76-77	0.5384707676106117
1103	78-79	0.5314457060343187
1103	80-81	0.5278133031973908
1103	82-83	0.5095457373679793
1103	84-85	0.47672360143299386
1103	86-87	0.4866975497474826
1103	88-89	0.4358376142946483
1103	90-91	0.47171719694426173
1103	92-93	0.4449023912615928
1103	94-95	0.39481825110197377
1103	96-97	0.48986091015423483
1103	98-99	0.49118700252314085
1103	100	0.5341672907940627
1104	1	0.3394380801770467
1104	2	0.4414421223637355
1104	3	0.361873143598082
1104	4	0.24762583486451462
1104	5	0.24083323899449027
1104	6	0.24916719255806186
1104	7	0.22703679623290896
1104	8	0.27398545573421984
1104	9	0.28878669625979114
1104	10-11	0.2595144359047481
1104	12-13	0.30419835065390544
1104	14-15	0.3728160772088174
1104	16-17	0.4188839468848755
1104	18-19	0.4177372401349828
1104	20-21	0.45907079897061465
1104	22-23	0.43514949306295847
1104	24-25	0.4555783837899341
1104	26-27	0.4494169283321128
1104	28-29	0.4707358336651595
1104	30-31	0.41527443991599
1104	32-33	0.39327991736966084
1104	34-35	0.3872598546685424
1104	36-37	0.4765953666259506
1104	38-39	0.47314604273726957
1104	40-41	0.5327919938107684
1104	42-43	0.3677683058858463
1104	44-45	0.41875242521783207
1104	46-47	0.5011081722267008
1104	48-49	0.47095171354720833
1104	50-51	0.42401506105762365
1104	52-53	0.4054629900024409
1104	54-55	0.4159265922363389
1104	56-57	0.4337756045582992
1104	58-59	0.40271319669492556
1104	60-61	0.39367017508897817
1104	62-63	0.36463607077801186
1104	64-65	0.336031726149983
1104	66-67	0.3642887063340936
1104	68-69	0.3955781782919061
1104	70-71	0.38692283224747115
1104	72-73	0.3590530578695734
1104	74-75	0.39539413627006326
1104	76-77	0.287315773321275
1104	78-79	0.2813805071229609
1104	80-81	0.36248759449394896
1104	82-83	0.35631729843516524
1104	84-85	0.2792788890678395
1104	86-87	0.26629410814884835
1104	88-89	0.3324070832168502
1104	90-91	0.35204325502397893
1104	92-93	0.35288045821474867
1104	94-95	0.29842906435680305
1104	96-97	0.32791430906066665
1104	98-99	0.31555366417743613
1104	100	0.3334950471463003
1105	1	0.1571497461422986
1105	2	-0.42094421991589925
1105	3	0.02687248984137014
1105	4	0.10920536176976015
1105	5	0.20969033791779168
1105	6	0.22038673856039992
1105	7	0.23705818439439952
1105	8	0.20688796365040218
1105	9	0.27708135857835003
1105	10-11	0.29888238980037585
1105	12-13	0.3230836556682988
1105	14-15	0.3397633776245499
1105	16-17	0.3488276923749396
1105	18-19	0.39274906935435894
1105	20-21	0.37973738441833405
1105	22-23	0.4020287941622982
1105	24-25	0.33851635021265025
1105	26-27	0.3798578356460425
1105	28-29	0.39575528793370296
1105	30-31	0.4236721463579869
1105	32-33	0.44660856819275097
1105	34-35	0.44520797219143304
1105	36-37	0.4474879328773085
1105	38-39	0.467702764002631
1105	40-41	0.4598973477055779
1105	42-43	0.4355803801613263
1105	44-45	0.3952047372528966
1105	46-47	0.4434106191931946
1105	48-49	0.4506620863974078
1105	50-51	0.45633763937065197
1105	52-53	0.4223700789305411
1105	54-55	0.4484986450217505
1105	56-57	0.5548497752467583
1105	58-59	0.4467132792320285
1105	60-61	0.4290119864224451
1105	62-63	0.4749984035827097
1105	64-65	0.3839723975893037
1105	66-67	0.37901307424210273
1105	68-69	0.4255748124207983
1105	70-71	0.42189368291144547
1105	72-73	0.3967690783260025
1105	74-75	0.3749306514086399
1105	76-77	0.34702561180965574
1105	78-79	0.286756508994344
1105	80-81	0.3566850157368009
1105	82-83	0.3362380825903557
1105	84-85	0.31955170916945974
1105	86-87	0.3617375303601378
1105	88-89	0.27561380068236474
1105	90-91	0.274735827186781
1105	92-93	0.2694313929600938
1105	94-95	0.27690001182948976
1105	96-97	0.23562466092170098
1105	98-99	0.22798020273516073
1105	100	0.29786847520870197
1106	1	0.2621346739649084
1106	2	0.31551326368205324
1106	3	0.25663594348350216
1106	4	0.1824507415310066
1106	5	0.20953620596826283
1106	6	0.1960677925732881
1106	7	0.19669788211403727
1106	8	0.22577669176673965
1106	9	0.24426787980677034
1106	10-11	0.3039054586648575
1106	12-13	0.2822857973983872
1106	14-15	0.3450206152105366
1106	16-17	0.3181874641058684
1106	18-19	0.40189767681938093
1106	20-21	0.36989166316020317
1106	22-23	0.33704984488185374
1106	24-25	0.311336426650513
1106	26-27	0.324209436436405
1106	28-29	0.33264000212852807
1106	30-31	0.3664747135661628
1106	32-33	0.30731937062056147
1106	34-35	0.27121704216015274
1106	36-37	0.3077776809706805
1106	38-39	0.2663160158352653
1106	40-41	0.3001989729962773
1106	42-43	0.39055243120071736
1106	44-45	0.3459504922333281
1106	46-47	0.30337640060222526
1106	48-49	0.21907698484057647
1106	50-51	0.2134192210570376
1106	52-53	0.24131134771759122
1106	54-55	0.23340711001632286
1106	56-57	0.24969098338959128
1106	58-59	0.18382713426131403
1106	60-61	0.17931525738502074
1106	62-63	0.16668075555936923
1106	64-65	0.18139039035493454
1106	66-67	0.1473274648736762
1106	68-69	0.1608424792525298
1106	70-71	0.16511564679912993
1106	72-73	0.18732310654228002
1106	74-75	0.20529076595459372
1106	76-77	0.23486181493309033
1106	78-79	0.15034261872490617
1106	80-81	0.1777424158328884
1106	82-83	0.10324090369933714
1106	84-85	0.14566123702040557
1106	86-87	0.16556880026261211
1106	88-89	0.18833425006262416
1106	90-91	0.2097599984916414
1106	92-93	0.21605502950147581
1106	94-95	0.21074964475378977
1106	96-97	0.1518691748079064
1106	98-99	0.20130958365997031
1106	100	0.21853880289612349
1107	1	0.06746183340970546
1107	2	-0.548870666478674
1107	3	-0.04883027540559226
1107	4	0.0684752555280852
1107	5	0.11280228970873907
1107	6	0.15769561052985637
1107	7	0.1658296956298031
1107	8	0.14796704274310457
1107	9	0.15138150395851113
1107	10-11	0.23899359087633343
1107	12-13	0.21923573102102978
1107	14-15	0.27696984844003225
1107	16-17	0.29731087764429276
1107	18-19	0.2826172519663501
1107	20-21	0.21475313582507738
1107	22-23	0.29569065791622506
1107	24-25	0.25971913473778585
1107	26-27	0.31571415348349774
1107	28-29	0.2793187876112668
1107	30-31	0.31808718212846543
1107	32-33	0.34434402469626946
1107	34-35	0.35461050738545197
1107	36-37	0.3840312721150596
1107	38-39	0.4492538107321806
1107	40-41	0.4419746509377305
1107	42-43	0.420268856119165
1107	44-45	0.42004062702405065
1107	46-47	0.44001134692556576
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2216	76-77	-0.8634688207898407
2216	78-79	-0.8411712041509105
2216	80-81	-0.7541465792850843
2216	82-83	-0.6592653798954657
2216	84-85	-0.760027420442281
2216	86-87	-0.75639272180414
2216	88-89	-0.6829360701212259
2216	90-91	-0.7698140002448
2216	92-93	-0.6986891691300912
2216	94-95	-0.6232087475857355
2216	96-97	-0.6741062185444804
2216	98-99	-0.7168666131147248
2216	100	-0.7795144251869459
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	8.0
13	70.0
14	273.0
15	847.0
16	2054.0
17	4328.0
18	7488.0
19	11527.0
20	16743.0
21	23643.0
22	31964.0
23	36993.0
24	37718.0
25	38936.0
26	41904.0
27	50509.0
28	63124.0
29	78666.0
30	97033.0
31	120774.0
32	149745.0
33	189512.0
34	259244.0
35	353156.0
36	524559.0
37	883260.0
38	730181.0
39	279851.0
40	541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.474581598566644	21.852904343997913	13.670316824006163	24.002197233429282
2	14.988438419495138	20.453822707890946	40.25006425854317	24.30767461407075
3	18.082195108228362	27.142018321154836	29.83873626217013	24.937050308446672
4	12.115312336681598	17.53232650689272	39.20672998255623	31.145631173869454
5	12.370137217387727	39.08396655188302	35.077915810622606	13.467980420106644
6	28.91237815565937	39.32910265460054	17.97538625349968	13.783132936240413
7	25.57553504813444	32.729685471084515	24.061396353624872	17.633383127156176
8	26.3490369439179	34.79057446285383	19.74678882164923	19.11359977157904
9	26.315750339062365	14.273211892166271	21.775437608559443	37.63560016021192
10-11	25.638604629490096	26.125634998374085	27.221168297867276	21.01459207426854
12-13	26.0633868166208	24.04055017329854	27.84966054107215	22.046402469008513
14-15	23.342456667056652	24.758572893139505	25.916319976680224	25.98265046312362
16-17	21.42280692796662	27.27755489713093	27.330832267124975	23.96880590777748
18-19	20.797212947026956	27.178103907743314	30.228995978839336	21.795687166390394
20-21	22.638537540565732	26.298949191572863	29.351353541832754	21.711159726028654
22-23	22.97512729810758	25.921795934391906	29.129046473723452	21.974030293777066
24-25	22.373989257076687	26.053460319789345	29.926330770206928	21.64621965292704
26-27	22.283795762781494	26.231844311708212	28.887662292517707	22.59669763299259
28-29	21.35222928547068	26.856639079019377	29.53587616887289	22.255255466637056
30-31	22.354731109665853	26.35740196933766	29.325907834521704	21.961959086474785
32-33	22.837200990617294	26.641391484181053	28.43398545006067	22.087422075140978
34-35	22.654981203415172	26.493809550660313	29.31894213675685	21.532267109167666
36-37	22.382874772967014	26.044463270226753	28.956718986699236	22.615942970106993
38-39	22.543842647303762	26.02187150527827	29.68619877382359	21.748087073594377
40-41	22.09838078470208	25.280040509195434	30.15856807033194	22.463010635770555
42-43	22.31899838039491	25.519506085509054	29.143696236490847	23.017799297605194
44-45	22.49801470063371	26.504242245187694	28.806569878572052	22.191173175606547
46-47	22.55251748847981	26.313470095096086	27.965358137100164	23.16865427932394
48-49	23.509489402070695	26.11525337189942	27.897162876668446	22.478094349361434
50-51	22.911296526891018	26.145079035238695	27.59106352561408	23.352560912256212
52-53	22.207866048555317	26.76461140677173	27.490509723753398	23.537012820919553
54-55	22.28844867629503	26.73292038462401	27.283700422949597	23.69493051613136
56-57	23.495077579810797	26.08749479354041	27.03625083851728	23.381176788131516
58-59	22.265457457053547	26.51890320502676	28.058815065954352	23.156824271965338
60-61	22.39957190866228	27.21903221912764	27.16114620018128	23.220249672028796
62-63	22.887886848345932	26.347289583052437	26.722711923891406	24.042111644710232
64-65	21.935476035627325	26.535546029202983	27.275299306805834	24.253678628363858
66-67	22.572952935764537	26.14465768581014	27.283701510116355	23.99868786830897
68-69	22.14698686870577	26.04933303772358	27.630903050204374	24.172777043366274
70-71	22.141197135840375	25.642603649437746	27.774860665368482	24.44133854935339
72-73	22.817075359088854	25.717049978982097	28.069270341759	23.39660432017005
74-75	22.89330959890905	26.218731457481027	27.48880570934848	23.399153234261444
76-77	22.549909351488566	26.149093347580312	27.023418657817082	24.27757864311404
78-79	22.264314714087412	26.252396902965287	27.189495650596267	24.29379273235103
80-81	21.802286961237556	26.707326564422466	27.81775495696642	23.67263151737355
82-83	22.844893770402493	25.525738511245205	28.900136358459534	22.72923135989277
84-85	23.11175694181947	26.102728393761193	27.281446550623695	23.504068113795647
86-87	21.56261409906941	26.85373729371694	27.804367672097648	23.779280935116002
88-89	22.584942132554456	25.57354041834997	27.638532556487732	24.202984892607844
90-91	22.151274122364278	25.57061395306296	27.90165190426493	24.37646002030783
92-93	22.53185588632877	25.92303773032333	27.491760345626126	24.05334603772177
94-95	21.179911559984028	25.846930512796934	28.26424633526157	24.708911591957467
96-97	21.62009122414956	25.72892071252198	27.785095296567718	24.865892766760737
98-99	20.904169472104314	26.55695747916974	27.64771659372839	24.891156454997557
100	21.78316306154838	25.662611332346746	26.82446635137488	25.729759254729995
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	339.0
1	229.0
2	177.0
3	241.5
4	174.5
5	124.0
6	266.5
7	714.0
8	1153.0
9	1262.5
10	1048.5
11	769.0
12	747.5
13	874.0
14	1073.0
15	1444.5
16	1862.5
17	2343.5
18	3052.5
19	3904.0
20	4835.5
21	5816.5
22	6905.0
23	8074.0
24	9109.0
25	10109.5
26	11782.5
27	13695.0
28	16710.0
29	24239.0
30	35510.5
31	45440.0
32	59069.0
33	72733.0
34	82837.0
35	94124.0
36	104495.5
37	124333.5
38	132389.0
39	122923.0
40	113341.5
41	102583.5
42	99865.5
43	106579.5
44	118112.5
45	142573.0
46	165994.0
47	181261.0
48	191000.5
49	186134.0
50	177158.0
51	180485.0
52	182230.5
53	174804.0
54	167418.5
55	144399.5
56	125343.0
57	103573.0
58	79703.5
59	77607.5
60	65104.5
61	43653.0
62	31474.0
63	25800.0
64	18528.0
65	8286.0
66	4146.0
67	2843.5
68	2037.0
69	1495.5
70	1174.0
71	856.5
72	632.0
73	550.5
74	424.5
75	295.5
76	194.0
77	110.0
78	59.0
79	32.5
80	12.0
81	6.5
82	4.0
83	1.0
84	1.5
85	2.5
86	1.5
87	0.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01219434816747698
2	0.004287850067019344
3	0.002106747142755169
4	0.027858632805374237
5	0.002701593394827217
6	0.0
7	4.7091994955703785E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.007733001276936621
16-17	0.0027263786553302187
18-19	0.0
20-21	1.2392630251500997E-5
22-23	7.931283360960638E-4
24-25	0.0
26-27	1.1153367226350896E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.346010167905269E-4
36-37	0.0
38-39	0.0
40-41	4.089567982995329E-4
42-43	7.559504453415607E-4
44-45	0.0
46-47	0.0
48-49	5.824536218205468E-4
50-51	0.0
52-53	0.0
54-55	3.5938627729352885E-4
56-57	1.1153367226350896E-4
58-59	2.3545997477851893E-4
60-61	7.435578150900598E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014251524789226145
70-71	8.550914873535686E-4
72-73	0.0
74-75	2.478526050300199E-4
76-77	0.002887482848599732
78-79	5.576683613175448E-4
80-81	0.0032716543863962632
82-83	0.0017225756049586384
84-85	0.00572539517619346
86-87	0.008253491747499663
88-89	0.00759668234417011
90-91	0.006233493016505001
92-93	0.008786374848314205
94-95	0.0014127598486711134
96-97	0.004907481579594394
98-99	0.0019952134704916604
100	0.08075037871878049
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4034656.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.100631759779848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83189946508686	15.212284634040724
2	15.15557495377696	6.698955623223342
3	6.1362722642185235	4.068464810677323
4	2.9362286102631185	2.595700291118213
5	1.686800909173618	1.863968287285399
6	1.0475941713111563	1.3891495808303753
7	0.6752978945760558	1.044715706732603
8	0.48056018967394915	0.8496547032315126
9	0.33936781335792776	0.6750218766730733
>10	1.9561483083158544	8.340342604549969
>50	0.2839547309555022	4.436115093972984
>100	0.34957623145545635	17.209987806227623
>500	0.06736805864331125	10.662834192368129
>1k	0.0501056941989353	19.37786593693007
>5k	0.002466052063482274	3.3587174596968645
>10k+	7.846529292898144E-4	2.216221392441853
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	18065	0.447745730986731	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13713	0.3398802772776663	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13654	0.3384179469079892	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11352	0.28136227723007856	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11310	0.2803212962889525	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11243	0.2786606838352514	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10123	0.25090119207188916	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9576	0.23734365457674708	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9360	0.23199003830809864	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7143	0.1770411157729432	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6885	0.1706465185631687	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6844	0.16963032288254562	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6305	0.15627106747142755	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6131	0.1519584321439052	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6109	0.15141315641283914	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5934	0.1470757358248138	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5933	0.1470509505643108	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5917	0.14665438639626277	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5821	0.14427500138797458	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5611	0.13907009668234419	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5589	0.13852482095127813	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5461	0.13535230760689387	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5438	0.1347822466153248	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5395	0.13371648041369574	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5304	0.13146102170792257	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5296	0.13126273962389853	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5261	0.13039525550629347	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5219	0.12935427456516738	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5046	0.12506642449814806	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4932	0.12224090480080582	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4853	0.12028286922106865	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4667	0.11567281076751029	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4581	0.11354127836425212	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4536	0.11242594164161702	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4476	0.11093882601143691	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4442	0.11009612715433485	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4434	0.10989784507031083	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4375	0.10843551470063371	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4298	0.10652704964190256	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4269	0.1058082770873155	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4232	0.10489122244870444	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4126	0.1022639848353862	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4046	0.10028116399514604	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.478526050300199E-5
2	0.0	0.0	0.0	0.0	2.478526050300199E-5
3	0.0	0.0	0.0	0.0	2.478526050300199E-5
4	0.0	0.0	0.0	0.0	2.478526050300199E-5
5	0.0	0.0	0.0	0.0	2.478526050300199E-5
6	0.0	0.0	0.0	0.0	4.957052100600398E-5
7	0.0	0.0	0.0	0.0	7.435578150900598E-5
8	0.0	0.0	0.0	0.0	9.914104201200796E-5
9	0.0	0.0	0.0	0.0	9.914104201200796E-5
10-11	0.0	0.0	0.0	1.2392630251500995E-5	9.914104201200796E-5
12-13	0.0	0.0	0.0	2.478526050300199E-5	3.9656416804803184E-4
14-15	0.0	0.0	0.0	2.478526050300199E-5	5.328831008145427E-4
16-17	0.0	0.0	0.0	4.957052100600398E-5	5.452757310660438E-4
18-19	0.0	0.0	0.0	4.957052100600398E-5	5.948462520720478E-4
20-21	0.0	0.0	0.0	4.957052100600398E-5	6.072388823235488E-4
22-23	2.478526050300199E-5	0.0	0.0	9.914104201200796E-5	7.063799243355567E-4
24-25	2.478526050300199E-5	0.0	0.0	2.9742312603602385E-4	7.931283360960637E-4
26-27	2.478526050300199E-5	0.0	0.0	5.452757310660438E-4	8.303062268505667E-4
28-29	2.478526050300199E-5	0.0	0.0	0.0016358271931981314	8.426988571020677E-4
30-31	2.478526050300199E-5	0.0	0.0	0.006307848798014007	8.550914873535686E-4
32-33	2.478526050300199E-5	0.0	0.0	0.01834109277222147	9.046620083595727E-4
34-35	2.478526050300199E-5	0.0	0.0	0.037351387578024	9.170546386110737E-4
36-37	2.478526050300199E-5	0.0	0.0	0.0696589746436871	9.170546386110737E-4
38-39	2.478526050300199E-5	0.0	0.0	0.1207290039101227	9.170546386110737E-4
40-41	2.478526050300199E-5	0.0	0.0	0.18096957956266904	9.170546386110737E-4
42-43	2.478526050300199E-5	0.0	0.0	0.24472966220664166	9.170546386110737E-4
44-45	2.478526050300199E-5	0.0	0.0	0.32151439924494185	9.914104201200796E-4
46-47	2.478526050300199E-5	0.0	0.0	0.42294807785347743	0.0010657662016290855
48-49	2.478526050300199E-5	0.0	0.0	0.5447924184862352	0.0011401219831380916
50-51	2.478526050300199E-5	0.0	0.0	0.6758816612866128	0.0011772998738925947
52-53	2.478526050300199E-5	0.0	0.0	0.8062025610113972	0.0013012261764076046
54-55	2.478526050300199E-5	0.0	0.0	0.9478998953070596	0.0013755819579166105
56-57	2.478526050300199E-5	0.0	0.0	1.125188863685033	0.0014003672184196125
58-59	2.478526050300199E-5	0.0	0.0	1.340027501725054	0.0014251524789226145
60-61	2.478526050300199E-5	0.0	0.0	1.5689937382517865	0.0014871156301801195
62-63	2.478526050300199E-5	0.0	0.0	1.8118273280299486	0.0015862566721921273
64-65	2.478526050300199E-5	0.0	0.0	2.0639801757572394	0.0015862566721921273
66-67	2.478526050300199E-5	0.0	0.0	2.3519105470206134	0.0015986493024436285
68-69	2.478526050300199E-5	0.0	0.0	2.6847146324246722	0.0016110419326951294
70-71	2.478526050300199E-5	0.0	0.0	3.054473541238708	0.0016482198234496325
72-73	2.478526050300199E-5	0.0	0.0	3.4380502327831666	0.0018465019074736483
74-75	2.478526050300199E-5	0.0	0.0	3.814910118731312	0.0018712871679766503
76-77	2.478526050300199E-5	0.0	0.0	4.249160275374159	0.0019084650587311532
78-79	2.478526050300199E-5	0.0	0.0	4.71863028719177	0.0019208576889826541
80-81	2.478526050300199E-5	0.0	0.0	5.243569712015101	0.001970428209988658
82-83	2.478526050300199E-5	0.0	0.0	5.786837836980402	0.0020943545125036685
84-85	2.478526050300199E-5	0.0	0.0	6.3096705146609775	0.002106747142755169
86-87	2.478526050300199E-5	0.0	0.0	6.869408445230523	0.002193495554515676
88	2.478526050300199E-5	0.0	0.0	7.318740432889446	0.002280243966276183
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9740	0.0	43.646275	1
GTATCAA	16705	0.0	38.39773	1
GGGCACC	4475	0.0	38.055275	94
GTGGTAT	2705	0.0	33.552723	1
GTACTAG	4665	0.0	33.26596	1
TATTCTC	2410	0.0	32.76168	5
ATCAACG	19625	0.0	31.192694	3
CAACGCA	19535	0.0	31.17924	5
TATCAAC	21405	0.0	29.807966	2
TAGGCAT	5915	0.0	29.636576	5
TACCCCG	335	0.0	29.461063	5
CTGTGCG	6315	0.0	29.325489	9
AACGCAG	22250	0.0	28.282234	6
TAGTACT	6565	0.0	28.072168	4
ACTGTGC	6745	0.0	27.800594	8
CTAGGCA	6540	0.0	26.813639	4
GCATCGT	5710	0.0	26.17304	8
ACGCAGA	23280	0.0	26.021545	7
TCAACGC	23755	0.0	25.946112	4
CGCAGAG	23360	0.0	25.872078	8
>>END_MODULE