FastQCFastQC Report
Mon 16 Jan 2017
ERR1146456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991385
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91160.30474178348825043No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA69400.23199955873282777No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC62970.21050449875225022No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA54410.18188899121978616No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC53770.17974951402109723No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC49350.16497374961765204No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC41950.1402360445078116No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT39790.13301530896223654No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC35120.11740381127805348No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC32000.10697385993444508No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA30870.10319634550550999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62700.038.295051
GGTATCA37550.035.55241
CAACGCA70550.032.5013965
ATCAACG72400.031.6751693
GTACATG140250.029.8004821
TATCAAC80600.029.5609172
TACATGG139400.029.4641322
GTACTAG19600.029.362711
AACGCAG82450.028.0732176
ACATGGG140900.027.8158743
GGGCACC22650.026.88938594
ACGCAGA85150.026.4671487
TCAACGC88800.026.0343064
CATGGGG105650.025.8849494
CGCAGAG85500.025.7551788
CGTTAAC6100.025.5197581
TAGGCAT24650.025.1614825
TAGTACT26850.025.0256634
ATGGGGG79750.024.9812625
CTAACGC10900.024.1434423
Produced by FastQC (version 0.11.5)