##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146456_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2991385
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45964160413989	31.0	30.0	34.0	26.0	34.0
2	30.55958293566358	31.0	30.0	34.0	26.0	34.0
3	30.738637453888416	31.0	31.0	34.0	27.0	34.0
4	34.19768702457223	37.0	35.0	37.0	30.0	37.0
5	34.438949516695445	37.0	35.0	37.0	30.0	37.0
6	34.543019704919296	37.0	35.0	37.0	30.0	37.0
7	34.43783264273907	37.0	35.0	37.0	30.0	37.0
8	34.36826720733038	37.0	35.0	37.0	30.0	37.0
9	35.722412862269486	39.0	35.0	39.0	30.0	39.0
10-11	35.648021067164535	39.0	35.0	39.0	29.5	39.0
12-13	35.649100333123286	39.0	35.0	39.0	30.0	39.0
14-15	36.66351155066967	40.0	36.0	41.0	29.5	41.0
16-17	36.5009651047926	40.0	36.0	41.0	28.0	41.0
18-19	36.44444947741598	40.0	36.0	41.0	27.5	41.0
20-21	36.792163997613144	40.0	36.0	41.0	30.0	41.0
22-23	36.515941946623386	39.5	35.5	41.0	28.0	41.0
24-25	36.649397519877915	39.5	36.0	41.0	29.5	41.0
26-27	36.7371075605447	40.0	36.0	41.0	30.0	41.0
28-29	36.56632663465251	39.5	35.5	41.0	29.0	41.0
30-31	36.49719327334997	39.0	35.0	41.0	29.0	41.0
32-33	36.59621061815848	39.0	35.0	41.0	29.5	41.0
34-35	36.643111301286865	39.0	35.0	41.0	30.0	41.0
36-37	36.56780638399938	39.0	35.0	41.0	29.5	41.0
38-39	36.410432625690106	39.0	35.0	41.0	29.0	41.0
40-41	36.36964048425729	39.0	35.0	41.0	29.0	41.0
42-43	36.20367237918222	39.0	35.0	41.0	28.0	41.0
44-45	36.02962975344197	39.0	35.0	41.0	28.0	41.0
46-47	35.91004367542125	39.0	34.5	40.5	27.5	41.0
48-49	35.79531504637484	38.0	34.0	40.0	27.5	41.0
50-51	35.58136214495961	38.0	34.0	40.0	27.0	41.0
52-53	35.40906168881639	38.0	34.0	40.0	27.0	41.0
54-55	35.227793647424186	38.0	33.5	40.0	26.0	41.0
56-57	35.09378264583128	37.5	33.0	40.0	26.0	41.0
58-59	34.8574491414512	37.0	33.0	40.0	26.0	41.0
60-61	34.595021536846644	36.5	33.0	40.0	26.0	41.0
62-63	34.33178912109274	36.0	33.0	39.5	26.0	41.0
64-65	34.04937294931946	35.5	32.5	39.0	26.0	41.0
66-67	33.74736317792595	35.0	32.0	39.0	25.0	41.0
68-69	33.46451593492647	35.0	32.0	38.5	25.0	40.0
70-71	33.36327487100456	35.0	32.0	38.0	25.0	40.0
72-73	33.13511935106982	35.0	32.0	37.0	25.0	40.0
74-75	32.84789303282593	35.0	32.0	37.0	25.0	39.0
76-77	32.59929965551074	35.0	31.0	36.0	24.5	39.0
78-79	32.364470805329304	35.0	31.0	36.0	24.0	39.0
80-81	32.10791539704853	35.0	31.0	35.5	24.0	37.5
82-83	31.86899028376488	35.0	31.0	35.0	24.0	37.0
84-85	31.656134700147256	34.5	31.0	35.0	23.5	37.0
86-87	31.474966612455432	34.5	31.0	35.0	23.0	36.0
88-89	31.4545249107019	35.0	31.0	35.0	23.0	36.0
90-91	31.360035234515117	35.0	31.0	35.0	23.0	36.0
92-93	31.20763709786604	34.5	31.0	35.0	23.0	36.0
94-95	31.149339018548265	34.0	31.0	35.0	23.0	35.0
96-97	31.043989322671607	34.0	31.0	35.0	21.0	35.0
98-99	30.93506703416645	34.0	31.0	35.0	20.0	35.0
100	30.421461296356036	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8079343633011575
1101	2	1.002195366033856
1101	3	0.9723100486331475
1101	4	1.0753755084785226
1101	5	1.005671518931031
1101	6	0.6615874495243901
1101	7	0.7000023386998961
1101	8	0.7569316777225978
1101	9	1.0221249847243854
1101	10-11	0.9700355653977581
1101	12-13	0.8680978077576
1101	14-15	1.1615449264704907
1101	16-17	1.2213427094172289
1101	18-19	1.3112354842282272
1101	20-21	1.0634573398372638
1101	22-23	1.3057400584106418
1101	24-25	1.1281636109954576
1101	26-27	1.0493555062737059
1101	28-29	1.3023030686960126
1101	30-31	1.3228394214840975
1101	32-33	1.0917944624527678
1101	34-35	1.0216006933241957
1101	36-37	0.9909424226385042
1101	38-39	1.0482802484165816
1101	40-41	0.9314934700595856
1101	42-43	1.0145262738878813
1101	44-45	0.9985658048420234
1101	46-47	0.9852383190518665
1101	48-49	0.9526580297846721
1101	50-51	0.9344595114520047
1101	52-53	1.0326538711164233
1101	54-55	1.01356577769468
1101	56-57	0.9743479754442887
1101	58-59	0.8909508563223341
1101	60-61	0.9163356598858812
1101	62-63	0.9035730982562811
1101	64-65	0.8818913402860957
1101	66-67	0.9118051709875346
1101	68-69	0.8733654846461008
1101	70-71	0.8240627639236067
1101	72-73	0.7684678454274376
1101	74-75	0.7907858632387601
1101	76-77	0.7835908003209582
1101	78-79	0.8055264126354444
1101	80-81	0.7830308110443909
1101	82-83	0.7669207967259588
1101	84-85	0.7967008895898111
1101	86-87	0.8330330475156202
1101	88-89	0.8330972419231735
1101	90-91	0.8337131053802587
1101	92-93	0.8045142540821217
1101	94-95	0.7829884426995655
1101	96-97	0.7029691524588841
1101	98-99	0.8299242135658353
1101	100	0.8057119457465838
1102	1	0.8241361991828704
1102	2	0.9485102492945039
1102	3	0.8720550981194073
1102	4	0.9809044599152159
1102	5	0.8657403541196516
1102	6	0.6583583530338686
1102	7	0.7245283311065336
1102	8	0.6605174609175108
1102	9	0.9102221254354035
1102	10-11	0.8437728073089303
1102	12-13	0.8087230698047918
1102	14-15	1.0774404337121055
1102	16-17	1.1793125768836603
1102	18-19	1.2193354171091428
1102	20-21	1.000354375021864
1102	22-23	1.2983179817772807
1102	24-25	1.186828930780166
1102	26-27	1.0779427208057015
1102	28-29	1.1815124435864774
1102	30-31	1.2229735946485434
1102	32-33	1.0941604564235021
1102	34-35	0.9888728018319952
1102	36-37	0.9572631482546541
1102	38-39	0.9994356512228464
1102	40-41	0.9910408935203066
1102	42-43	1.039904768169265
1102	44-45	1.015530187124682
1102	46-47	0.9767732039496124
1102	48-49	0.9234722538302051
1102	50-51	1.0208475431637396
1102	52-53	0.930507155488776
1102	54-55	1.0263110782462164
1102	56-57	0.9772187501309304
1102	58-59	0.8897154811497572
1102	60-61	0.9790363502228843
1102	62-63	0.881105281089603
1102	64-65	0.7911462184454336
1102	66-67	0.8708153767585571
1102	68-69	0.9626311664628417
1102	70-71	0.9149630452057238
1102	72-73	0.7992440431590637
1102	74-75	0.82570341297167
1102	76-77	0.8080001856320607
1102	78-79	0.7570420373612095
1102	80-81	0.8566192889032678
1102	82-83	0.8306763012484062
1102	84-85	0.7703301349293916
1102	86-87	0.8337819930889552
1102	88-89	0.8773385090042716
1102	90-91	0.8132748617131291
1102	92-93	0.8679917166857223
1102	94-95	0.8027946729086715
1102	96-97	0.8569956499712568
1102	98-99	0.901784586269514
1102	100	0.8943567897853448
1103	1	0.5389972074328782
1103	2	0.7151277610971327
1103	3	0.6147625189017951
1103	4	0.6820806083731057
1103	5	0.6453868905951126
1103	6	0.46034289874462075
1103	7	0.44706405212828315
1103	8	0.45932623830131547
1103	9	0.5087091751313295
1103	10-11	0.6801740595563999
1103	12-13	0.5550522937914977
1103	14-15	0.7680900862418341
1103	16-17	0.9550989636807401
1103	18-19	1.0386789120272581
1103	20-21	0.7446971599252734
1103	22-23	0.9557227797289372
1103	24-25	0.8936290510821436
1103	26-27	0.7813491892945237
1103	28-29	0.9060244086086016
1103	30-31	0.9354584203599572
1103	32-33	0.7589100105706805
1103	34-35	0.7837604046839957
1103	36-37	0.699112798454081
1103	38-39	0.7741681577349482
1103	40-41	0.6773842158502319
1103	42-43	0.7128338373363476
1103	44-45	0.761013728983869
1103	46-47	0.6861245709281718
1103	48-49	0.691482268314509
1103	50-51	0.7729428147571014
1103	52-53	0.8038913622858459
1103	54-55	0.7886259009500804
1103	56-57	0.7156824676514049
1103	58-59	0.6834088756827086
1103	60-61	0.6582102717833678
1103	62-63	0.5567972905477347
1103	64-65	0.6459529451611346
1103	66-67	0.6601716373769193
1103	68-69	0.6294179166418061
1103	70-71	0.5661900598451339
1103	72-73	0.5632234316143041
1103	74-75	0.52139941307653
1103	76-77	0.5434763797493574
1103	78-79	0.4927509706569637
1103	80-81	0.563987275323413
1103	82-83	0.47494488051262707
1103	84-85	0.5099304585504534
1103	86-87	0.5222734922103527
1103	88-89	0.536074347852054
1103	90-91	0.6319416215919809
1103	92-93	0.6100786938509231
1103	94-95	0.5097525985297153
1103	96-97	0.5186814360594809
1103	98-99	0.5739865275589473
1103	100	0.6626200792579731
1104	1	0.4665002523171786
1104	2	0.7136532332504721
1104	3	0.6747335570544237
1104	4	0.7800832533270565
1104	5	0.7251016686601304
1104	6	0.37884546015027354
1104	7	0.48223542456784685
1104	8	0.4806260154511719
1104	9	0.6740005173013301
1104	10-11	0.6451621202351916
1104	12-13	0.5660819580070182
1104	14-15	0.6812619381759717
1104	16-17	0.8508347429519958
1104	18-19	0.8277282495818312
1104	20-21	0.70159148808564
1104	22-23	0.9813034403955996
1104	24-25	0.8514979531403384
1104	26-27	0.7241924280811816
1104	28-29	0.9762694500230111
1104	30-31	0.9915881116905965
1104	32-33	0.8335881972937429
1104	34-35	0.6431762274894268
1104	36-37	0.6668675381501572
1104	38-39	0.7919598203882501
1104	40-41	0.768166389619914
1104	42-43	0.7285254965445915
1104	44-45	0.7924177880015435
1104	46-47	0.6813269769316506
1104	48-49	0.6538481779843863
1104	50-51	0.8226544032223302
1104	52-53	0.7975830791459302
1104	54-55	0.7903385121495887
1104	56-57	0.7136354757767975
1104	58-59	0.6665622345000344
1104	60-61	0.6929976970263922
1104	62-63	0.6743733915631296
1104	64-65	0.7279657349641653
1104	66-67	0.7701830435357735
1104	68-69	0.6782687818764117
1104	70-71	0.5855247997896313
1104	72-73	0.580385939263131
1104	74-75	0.6020490180793914
1104	76-77	0.5921031293128038
1104	78-79	0.500536242392819
1104	80-81	0.5609419223078405
1104	82-83	0.5540885303850338
1104	84-85	0.48654176304521357
1104	86-87	0.4897031786429338
1104	88-89	0.43253307182933654
1104	90-91	0.48382288786075733
1104	92-93	0.5141429845586885
1104	94-95	0.4640924111331124
1104	96-97	0.4264317885911133
1104	98-99	0.48035529825999745
1104	100	0.5825691394040788
1105	1	0.5294053426138312
1105	2	0.6829433909446685
1105	3	0.7031196391664736
1105	4	0.7594664163326286
1105	5	0.6075731988619211
1105	6	0.3689323477653659
1105	7	0.4225458248472762
1105	8	0.44250571129272487
1105	9	0.5305219676947388
1105	10-11	0.6200393425967476
1105	12-13	0.5407188934527198
1105	14-15	0.6310240455356748
1105	16-17	0.7754193361445232
1105	18-19	0.8200333153294039
1105	20-21	0.6530417148686496
1105	22-23	0.8368230666995586
1105	24-25	0.6946863681122579
1105	26-27	0.526410420018756
1105	28-29	0.7666004248397726
1105	30-31	0.8601108026195092
1105	32-33	0.7238619864026035
1105	34-35	0.5844636736128521
1105	36-37	0.5300521642944176
1105	38-39	0.671693855762328
1105	40-41	0.573915639600564
1105	42-43	0.5522281369745912
1105	44-45	0.6498690671199867
1105	46-47	0.5856339181871064
1105	48-49	0.5726095015590005
1105	50-51	0.7219714880421861
1105	52-53	0.6134863252058906
1105	54-55	0.6465547699919085
1105	56-57	0.5419269135848523
1105	58-59	0.580589027374387
1105	60-61	0.5811232504489894
1105	62-63	0.5514466539600207
1105	64-65	0.5179069895158577
1105	66-67	0.5950204464452753
1105	68-69	0.5629551482507935
1105	70-71	0.5373837005244937
1105	72-73	0.5125616988536166
1105	74-75	0.4775045093085879
1105	76-77	0.492079964756563
1105	78-79	0.5193584707992542
1105	80-81	0.5752297795400807
1105	82-83	0.5419615605443546
1105	84-85	0.5697799340707768
1105	86-87	0.6107925783137844
1105	88-89	0.5956010444766413
1105	90-91	0.6021257136358464
1105	92-93	0.6912356112260127
1105	94-95	0.6243579833174664
1105	96-97	0.663600226186194
1105	98-99	0.5953049208405332
1105	100	0.7050062798837899
1106	1	0.49578165707784905
1106	2	0.5836034123735914
1106	3	0.5598705698265647
1106	4	0.6879373285850008
1106	5	0.508615288500117
1106	6	0.30760577159725244
1106	7	0.33234630922111563
1106	8	0.4113592976775138
1106	9	0.44267166661965973
1106	10-11	0.46590722021205266
1106	12-13	0.34467435136861724
1106	14-15	0.5281859127159123
1106	16-17	0.6382520801755192
1106	18-19	0.6322099020941607
1106	20-21	0.45909398948592894
1106	22-23	0.7801929559937477
1106	24-25	0.6014878134072887
1106	26-27	0.441382430436704
1106	28-29	0.8063105523262806
1106	30-31	0.6891510582180942
1106	32-33	0.5881592551058148
1106	34-35	0.5691716880826334
1106	36-37	0.5375158190741871
1106	38-39	0.6169454730651935
1106	40-41	0.5761206668832344
1106	42-43	0.538060545643944
1106	44-45	0.5659128041117469
1106	46-47	0.5567073994133764
1106	48-49	0.49329703638731104
1106	50-51	0.5932581925033062
1106	52-53	0.5878655239763191
1106	54-55	0.6324127059792204
1106	56-57	0.45856416071888617
1106	58-59	0.4905369249963769
1106	60-61	0.5251550266299532
1106	62-63	0.3697935581485652
1106	64-65	0.5159262379551492
1106	66-67	0.6080310331289667
1106	68-69	0.5485084395425943
1106	70-71	0.47997157025157833
1106	72-73	0.368457534492471
1106	74-75	0.38013399136912795
1106	76-77	0.4365845573595024
1106	78-79	0.41888946944897754
1106	80-81	0.5050692786323836
1106	82-83	0.43478981485310797
1106	84-85	0.46752521267410074
1106	86-87	0.43713458237936464
1106	88-89	0.4691899062626206
1106	90-91	0.4151685460909391
1106	92-93	0.36507433215589913
1106	94-95	0.46500594519255856
1106	96-97	0.5458920098822873
1106	98-99	0.42537720943611745
1106	100	0.36375260524741293
1107	1	0.3662073547627074
1107	2	0.5155530430862711
1107	3	0.4370417651501306
1107	4	0.5851586361443353
1107	5	0.4530428128255011
1107	6	0.1302718318859064
1107	7	0.14203355493301473
1107	8	0.2480169538086372
1107	9	0.2652389982424239
1107	10-11	0.24296999708828793
1107	12-13	0.21324388605497546
1107	14-15	0.38038303518495553
1107	16-17	0.5087382188447549
1107	18-19	0.5514213836567095
1107	20-21	0.30873066616460676
1107	22-23	0.6311249143209992
1107	24-25	0.2611504433210783
1107	26-27	0.28771407691503015
1107	28-29	0.4901462346878347
1107	30-31	0.49576309136126184
1107	32-33	0.355947167256808
1107	34-35	0.2101451692059655
1107	36-37	0.19095682661372848
1107	38-39	0.21693152197693877
1107	40-41	0.0913328914053082
1107	42-43	0.32744817983505925
1107	44-45	0.25404515160479235
1107	46-47	0.20112446042256948
1107	48-49	0.3199650404909846
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2216	100	-1.2125528776839438
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12305.0
1	1989.0
2	327.0
3	314.0
4	314.0
5	164.0
6	145.0
7	190.0
8	414.0
9	769.0
10	1212.0
11	2038.0
12	3173.0
13	4384.0
14	6420.0
15	8490.0
16	10911.0
17	12954.0
18	14932.0
19	17039.0
20	19118.0
21	22244.0
22	24924.0
23	28507.0
24	31811.0
25	35685.0
26	40305.0
27	46586.0
28	54645.0
29	65086.0
30	77263.0
31	93400.0
32	114215.0
33	144831.0
34	196701.0
35	263538.0
36	383353.0
37	576814.0
38	513595.0
39	160197.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.895563647854885	20.531557378986324	12.172942175021895	24.399936798136896
2	15.445424467780395	20.878817560636215	40.379852970755664	23.295905000827723
3	18.12861057608654	28.095362540636305	29.4068090955446	24.369217787732556
4	12.093594677641505	17.4810754205658	40.02979703957411	30.395532862218584
5	12.937085593554084	38.855641433778736	35.110577212005886	13.096695760661298
6	28.933577033918432	38.53817272262173	19.226155464266746	13.302094779193094
7	26.05637186305476	32.464873840145444	24.473590577430087	17.00516371936971
8	25.53364129991059	34.79743453022329	21.2339695937603	18.43495457610581
9	26.922494022686074	15.25198809745836	21.38418292680878	36.44133495304679
10-11	24.995576359595166	26.084001948586295	28.228791185856583	20.691630505961957
12-13	25.67785540977827	24.24272111074908	27.85094835146499	22.22847512800766
14-15	23.09357132259028	25.245123120691936	25.46034047291022	26.200965083807564
16-17	22.244965518168495	27.655084221705405	26.67601529837203	23.42393496175407
18-19	21.9715571065896	28.002044022092377	27.940777131687856	22.085621739630167
20-21	23.499573524795718	27.1211512209155	26.806554342485466	22.572720911803316
22-23	23.70142947992006	26.931608636073044	26.602281671810545	22.764680212196353
24-25	23.14974601803667	27.17746475086579	27.10421914198961	22.56857008910793
26-27	22.83791486678752	27.495853411089122	26.577950747612384	23.088280974510972
28-29	22.46819413234769	27.86883675998068	26.76003948169771	22.90292962597392
30-31	23.042956107054916	27.509217169001815	26.639819379778277	22.80800734416499
32-33	23.233836070201143	27.801245178943017	25.950748980762818	23.014169770093023
34-35	23.352063921492846	27.45060115441813	26.72235991967804	22.474975004410986
36-37	23.200614050213765	27.320915186246996	26.574159134736426	22.904311628802816
38-39	23.278225479839627	27.28122751290031	26.928038256091703	22.512508751168365
40-41	22.96751239859704	26.756718602685257	27.32709608135429	22.948672917363414
42-43	22.824728452899542	26.96579967770456	26.80744166692855	23.402030202467348
44-45	22.762706243946237	27.733099421141276	26.762950957601845	22.741243377310642
46-47	22.86258973628951	27.330521649483842	26.480915586626676	23.325973027599968
48-49	23.271866636736462	26.829416643255687	26.81498360783544	23.083733112172407
50-51	23.127746826398305	27.03160334419418	26.40465515917288	23.43599467023463
52-53	22.635549887164284	27.399787939433352	26.450237181846138	23.514424991556226
54-55	22.676063651813667	27.402223084512535	26.423004486467743	23.498708777206048
56-57	23.253669814258107	26.935204155445774	26.350443422412123	23.460682607883996
58-59	22.6445829646975	26.90424038333086	27.15406415229722	23.297112499674423
60-61	22.70358689314597	27.55352951159309	26.47799925731636	23.26488433794458
62-63	23.036688888354963	26.977785984592956	26.17004942963288	23.815475697419206
64-65	22.280051856787214	27.02788856672108	26.530291942285377	24.161767634206324
66-67	22.848210594944835	26.647852344256727	26.516801822844027	23.98713523795441
68-69	22.32726341320023	26.805038907108493	26.664079526166727	24.20361815352455
70-71	22.3195172327222	26.511634706854924	26.84798950874151	24.32085855168136
72-73	22.751073878072386	26.596230415305676	27.006859274876653	23.64583643174528
74-75	22.763815455928153	26.94438427530203	26.7041987680011	23.587601500768717
76-77	22.4504897343468	26.728150475318042	26.762057358079193	24.059302432255965
78-79	22.265658352342594	26.77572704115076	26.75873467914185	24.199879927364794
80-81	22.098574954362512	26.909196188765783	27.20286568464295	23.78936317222875
82-83	22.653764478667167	26.050208507593748	27.796201260152753	23.499825753586336
84-85	22.80516315161162	26.54265387552296	26.75993616975511	23.89224680311031
86-87	21.879941894862604	26.922777409386022	27.215511199465205	23.981769496286173
88-89	22.537943156035713	25.9809280767947	27.088316440133003	24.392812327036584
90-91	22.302022720413458	25.995315051150165	27.27946825053222	24.423193977904155
92-93	22.44918478853237	26.17746148946874	27.23811596698496	24.13523775501393
94-95	21.637844541856058	26.059627332131562	27.617392609271622	24.68513551674075
96-97	21.957562119166667	25.867373553490253	27.33165601486639	24.843408312476694
98-99	21.504927741940936	26.520790297438758	27.21663054819809	24.75765141242222
100	22.078935434096902	25.861269582296163	26.68416702205815	25.37562796154878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10500.0
1	6270.5
2	2019.0
3	1668.5
4	1082.0
5	716.5
6	601.0
7	578.0
8	556.5
9	538.0
10	532.5
11	510.0
12	464.5
13	464.0
14	482.0
15	531.0
16	612.0
17	684.0
18	784.0
19	884.0
20	1081.5
21	1394.5
22	1853.0
23	2521.0
24	3292.5
25	4313.0
26	5928.0
27	7891.0
28	10689.0
29	15922.5
30	23767.0
31	32206.5
32	41715.5
33	50988.0
34	60916.0
35	70491.0
36	79500.0
37	93784.0
38	99441.0
39	93805.5
40	88289.5
41	84146.0
42	84572.5
43	89668.0
44	96841.5
45	108523.5
46	119392.5
47	127078.5
48	131510.0
49	128844.0
50	123548.5
51	123128.5
52	123235.5
53	119591.0
54	114854.5
55	104489.5
56	93883.0
57	80027.5
58	66629.0
59	60538.5
60	50263.5
61	38219.0
62	29509.0
63	22956.5
64	16772.5
65	10769.5
66	7372.5
67	5379.5
68	3916.5
69	2912.0
70	2158.0
71	1517.5
72	1107.0
73	841.5
74	615.0
75	441.0
76	321.0
77	217.0
78	134.0
79	84.5
80	79.5
81	62.5
82	35.0
83	22.5
84	22.0
85	21.0
86	19.0
87	18.0
88	16.0
89	10.5
90	7.5
91	9.0
92	9.5
93	4.0
94	2.5
95	3.0
96	2.5
97	1.0
98	0.5
99	1.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9843935167155013
2	0.445880419939259
3	0.4802792017744289
4	0.4649351387400819
5	0.4305029275736824
6	0.48499273747779037
7	0.5028440003543508
8	0.5043817495909085
9	0.5198261006189441
10-11	0.5313425052275117
12-13	0.5308410652590689
14-15	0.5277989961171832
16-17	0.5316935132054216
18-19	0.5308243505934542
20-21	0.5292197426944375
22-23	0.49167860372369315
24-25	0.5275148468017323
26-27	0.5062370774741466
28-29	0.5122209277642297
30-31	0.5153799995654187
32-33	0.5184053540416897
34-35	0.5301891933000935
36-37	0.5477061628643588
38-39	0.5371424941958324
40-41	0.543226632479604
42-43	0.5403684246594805
44-45	0.5508986639967774
46-47	0.5488427601261623
48-49	0.520594975237223
50-51	0.5355211716312009
52-53	0.5288520200509129
54-55	0.5383626647857096
56-57	0.5256260895872648
58-59	0.5311084999089051
60-61	0.5245062069910761
62-63	0.5194416633098047
64-65	0.5309413532527575
66-67	0.5286848733947653
68-69	0.548725757466859
70-71	0.533381694432512
72-73	0.5372260675239061
74-75	0.5340837103883318
76-77	0.5210964152056655
78-79	0.5522191225803432
80-81	0.5268796895083716
82-83	0.5253085109405844
84-85	0.5346352943536188
86-87	0.8370035953245737
88-89	1.0980699575614639
90-91	1.0880578728582244
92-93	1.0881748755175278
94-95	1.0928549818896598
96-97	1.086302832968675
98-99	1.082191025227445
100	1.0766584709089602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2991385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.66442068483761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37808165755355	32.65577366650865
2	11.544282029951104	9.619716460005879
3	3.950178937627666	4.937457511131119
4	1.8770030689983432	3.1281698197391297
5	1.0311179515491773	2.1480466054516465
6	0.6023100068241748	1.50569385042059
7	0.41128872074711254	1.1995274398895477
8	0.31920745697140196	1.0639675018394952
9	0.225698611384693	0.8463241703443973
>10	1.32620394792715	10.472926799862702
>50	0.13954612735701807	4.1211270542337415
>100	0.1631357756241679	14.606556142172083
>500	0.02199277349435597	6.179248300068687
>1k	0.00955160600667036	6.406153599059509
>5k	4.013279834735445E-4	1.10931107927272
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9116	0.30474178348825043	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6940	0.23199955873282777	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6297	0.21050449875225022	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5441	0.18188899121978616	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5377	0.17974951402109723	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	4935	0.16497374961765204	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4195	0.1402360445078116	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	3979	0.13301530896223654	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3512	0.11740381127805348	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3200	0.10697385993444508	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	3087	0.10319634550550999	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.342933122951409E-5
2	0.0	0.0	0.0	0.0	3.342933122951409E-5
3	0.0	0.0	0.0	0.0	3.342933122951409E-5
4	0.0	0.0	0.0	0.0	3.342933122951409E-5
5	0.0	0.0	0.0	0.0	3.342933122951409E-5
6	0.0	0.0	0.0	0.0	1.6714665614757043E-4
7	0.0	0.0	0.0	0.0	1.6714665614757043E-4
8	0.0	0.0	0.0	0.0	1.6714665614757043E-4
9	0.0	0.0	0.0	0.0	2.3400531860659862E-4
10-11	0.0	0.0	0.0	0.0	2.3400531860659862E-4
12-13	0.0	0.0	0.0	0.0	2.5071998422135567E-4
14-15	0.0	0.0	0.0	0.0	3.3429331229514086E-4
16-17	0.0	0.0	0.0	0.0	4.0115197475416907E-4
18-19	0.0	0.0	0.0	1.6714665614757044E-5	4.6801063721319723E-4
20-21	0.0	0.0	0.0	3.342933122951409E-5	5.515839652869824E-4
22-23	0.0	0.0	0.0	6.685866245902817E-5	5.682986309017394E-4
24-25	0.0	0.0	0.0	1.6714665614757043E-4	5.682986309017394E-4
26-27	0.0	0.0	0.0	9.861652712706654E-4	5.682986309017394E-4
28-29	0.0	0.0	0.0	0.0027244904952053984	5.682986309017394E-4
30-31	0.0	0.0	0.0	0.011900841917707017	5.682986309017394E-4
32-33	0.0	0.0	0.0	0.03368005121373545	5.682986309017394E-4
34-35	1.6714665614757044E-5	0.0	0.0	0.06879756367034	6.017279621312536E-4
36-37	3.342933122951409E-5	0.0	0.0	0.12263550161547243	6.017279621312536E-4
38-39	3.342933122951409E-5	0.0	0.0	0.20971890946835664	6.853012902050387E-4
40-41	3.342933122951409E-5	0.0	0.0	0.31497115884448174	7.020159558197959E-4
42-43	3.342933122951409E-5	0.0	0.0	0.42204530677261537	7.187306214345529E-4
44-45	3.342933122951409E-5	0.0	0.0	0.5502467920378019	7.52159952664067E-4
46-47	3.342933122951409E-5	0.0	0.0	0.7053923182739767	8.023039495083381E-4
48-49	3.342933122951409E-5	0.0	0.0	0.8829522110995409	8.691626119673663E-4
50-51	3.342933122951409E-5	0.0	0.0	1.0836117718046991	9.025919431968804E-4
52-53	3.342933122951409E-5	0.0	0.0	1.2856419350902675	0.0010028799368854227
54-55	3.342933122951409E-5	0.0	0.0	1.4968985937951818	0.0010697385993444508
56-57	3.342933122951409E-5	0.0	0.0	1.7460641141143651	0.001119882596188722
58-59	3.342933122951409E-5	0.0	0.0	2.041495828855196	0.0011700265930329932
60-61	3.342933122951409E-5	1.6714665614757044E-5	0.0	2.37386695460464	0.0011700265930329932
62-63	3.342933122951409E-5	3.342933122951409E-5	0.0	2.7213648527354386	0.0012703145867215353
64-65	3.342933122951409E-5	3.342933122951409E-5	0.0	3.0757491930995173	0.0013037439179510494
66-67	3.342933122951409E-5	3.342933122951409E-5	0.0	3.4672902351252013	0.0013037439179510494
68-69	3.342933122951409E-5	3.342933122951409E-5	0.0	3.909643860619746	0.0013037439179510494
70-71	3.342933122951409E-5	3.342933122951409E-5	0.0	4.411167402390531	0.0013204585835658064
72-73	3.342933122951409E-5	3.342933122951409E-5	0.0	4.953675304248701	0.0014207465772543488
74-75	3.342933122951409E-5	3.342933122951409E-5	0.0	5.480254129776007	0.0014374612428691058
76-77	3.342933122951409E-5	3.342933122951409E-5	0.0	6.037704942693769	0.0014374612428691058
78-79	3.342933122951409E-5	3.342933122951409E-5	0.0	6.643594856563096	0.0014374612428691058
80-81	3.342933122951409E-5	5.0143996844271134E-5	0.0	7.3240321790742415	0.0014374612428691058
82-83	3.342933122951409E-5	6.685866245902817E-5	0.0	8.051370853300394	0.0014876052397133769
84-85	3.342933122951409E-5	6.685866245902817E-5	0.0	8.761342989952816	0.001504319905328134
86-87	3.342933122951409E-5	8.357332807378521E-5	0.0	9.494398079819213	0.0016046078990166763
88	3.342933122951409E-5	1.0028799368854227E-4	0.0	10.05671286043087	0.0017048958927052184
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6270	0.0	38.29505	1
GGTATCA	3755	0.0	35.5524	1
CAACGCA	7055	0.0	32.501396	5
ATCAACG	7240	0.0	31.675169	3
GTACATG	14025	0.0	29.800482	1
TATCAAC	8060	0.0	29.560917	2
TACATGG	13940	0.0	29.464132	2
GTACTAG	1960	0.0	29.36271	1
AACGCAG	8245	0.0	28.073217	6
ACATGGG	14090	0.0	27.815874	3
GGGCACC	2265	0.0	26.889385	94
ACGCAGA	8515	0.0	26.467148	7
TCAACGC	8880	0.0	26.034306	4
CATGGGG	10565	0.0	25.884949	4
CGCAGAG	8550	0.0	25.755178	8
CGTTAAC	610	0.0	25.519758	1
TAGGCAT	2465	0.0	25.161482	5
TAGTACT	2685	0.0	25.025663	4
ATGGGGG	7975	0.0	24.981262	5
CTAACGC	1090	0.0	24.143442	3
>>END_MODULE