FastQCFastQC Report
Mon 16 Jan 2017
ERR1146456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991385
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78680.26302197811381683No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA74710.24975053361569974No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC68840.23012751618397498No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67080.22424395388758048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60470.2021471659448717No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57810.19325496383782095No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC54980.18379446309986844No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45730.15287233171256792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44400.14842623065904254No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC41410.13843086062141782No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT40350.13488735151108933No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA35260.11787182191526667No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG31240.10443323076100201No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC30130.10072257499452594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.048.8200721
GTATCAA105350.045.301711
CAACGCA133100.035.167915
ATCAACG134450.034.787983
TATCAAC137900.034.430122
AACGCAG146900.032.4720346
TCAACGC151900.030.9772764
ACGCAGA159150.029.2934327
CGCAGAG161600.028.7911578
GGGCACC22950.027.25547494
GTACATG126000.027.017871
TAGTACT24550.026.8067054
TATTCTC18150.026.6701765
TACATGG127550.026.277892
GCAGAGT174350.026.1779219
ACATGGG126650.026.0183493
CTGTGCG24950.025.9983869
CTAGTAC29000.024.63843
CATGGGG96200.024.1878384
GTACTGT30100.023.10796
Produced by FastQC (version 0.11.5)