##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2991385
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82691662891938	31.0	31.0	34.0	30.0	34.0
2	31.771074602567037	31.0	31.0	34.0	30.0	34.0
3	31.95593713279969	31.0	31.0	34.0	30.0	34.0
4	35.70814254935423	37.0	35.0	37.0	33.0	37.0
5	35.623089304786916	37.0	35.0	37.0	33.0	37.0
6	35.654852183854636	37.0	35.0	37.0	33.0	37.0
7	35.568555702458895	37.0	35.0	37.0	33.0	37.0
8	35.5480417933499	37.0	35.0	37.0	33.0	37.0
9	37.130248697509685	39.0	37.0	39.0	33.0	39.0
10-11	37.078208923291385	39.0	37.0	39.0	33.0	39.0
12-13	37.02298667674004	39.0	37.0	39.0	33.0	39.0
14-15	38.202051892350866	40.0	37.0	41.0	33.0	41.0
16-17	38.20112038403616	40.0	37.0	41.0	33.0	41.0
18-19	38.262393506686706	40.0	37.5	41.0	33.0	41.0
20-21	38.278549902469926	40.0	38.0	41.0	33.0	41.0
22-23	38.246082165953226	40.0	37.5	41.0	33.0	41.0
24-25	38.18039202576733	40.0	37.0	41.0	33.0	41.0
26-27	38.031989697080114	40.0	37.0	41.0	33.0	41.0
28-29	37.96279081428837	40.0	37.0	41.0	33.0	41.0
30-31	37.82256596860651	40.0	37.0	41.0	33.0	41.0
32-33	37.68625653334492	40.0	36.0	41.0	32.0	41.0
34-35	37.5780503011147	40.0	36.0	41.0	32.0	41.0
36-37	37.48171148147096	40.0	36.0	41.0	32.0	41.0
38-39	37.370897928551486	40.0	36.0	41.0	31.0	41.0
40-41	37.24743103946834	39.5	36.0	41.0	31.0	41.0
42-43	37.12199683424233	39.0	35.0	41.0	31.0	41.0
44-45	36.93322842094882	39.0	35.0	41.0	30.5	41.0
46-47	36.69903974246043	39.0	35.0	41.0	30.0	41.0
48-49	36.45466280669322	39.0	35.0	41.0	30.0	41.0
50-51	36.27437123606624	39.0	35.0	41.0	29.0	41.0
52-53	36.09543021042093	38.5	35.0	41.0	29.0	41.0
54-55	35.908187511804734	38.0	35.0	40.0	28.5	41.0
56-57	35.66997427612962	38.0	34.0	40.0	28.0	41.0
58-59	35.4215430310709	37.5	34.0	40.0	28.0	41.0
60-61	35.16292118868016	37.0	34.0	40.0	28.0	41.0
62-63	34.8979893928732	36.5	34.0	40.0	27.0	41.0
64-65	34.636248092438784	36.0	33.5	39.0	27.0	41.0
66-67	34.39718508316382	35.5	33.0	39.0	26.5	41.0
68-69	34.193898979903956	35.0	33.0	39.0	26.0	41.0
70-71	33.94865238008481	35.0	33.0	38.0	26.0	40.5
72-73	33.72026552917795	35.0	33.0	37.5	26.0	40.0
74-75	33.51402210013087	35.0	33.0	37.0	26.0	39.5
76-77	33.33977171109703	35.0	33.0	36.5	26.0	39.0
78-79	33.20133232599615	35.0	33.0	36.0	26.0	39.0
80-81	33.02955587461995	35.0	33.0	36.0	26.0	38.0
82-83	32.830934500239856	35.0	33.0	35.5	26.0	37.0
84-85	32.66701578031581	35.0	33.0	35.0	26.0	37.0
86-87	32.524895157259934	35.0	33.0	35.0	26.0	36.5
88-89	32.398554014277664	35.0	33.0	35.0	26.0	36.0
90-91	32.29654641579067	35.0	33.0	35.0	26.0	36.0
92-93	32.21902713960256	35.0	33.0	35.0	25.0	36.0
94-95	32.16566122381438	35.0	32.0	35.0	25.0	35.0
96-97	32.106002069275604	35.0	32.0	35.0	25.0	35.0
98-99	32.062414901458695	35.0	32.0	35.0	25.0	35.0
100	31.558418926350168	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48453881886743133
1101	2	0.5232122805118458
1101	3	0.4736434128829501
1101	4	0.32050945044065315
1101	5	0.34704553359551227
1101	6	0.39754412824235175
1101	7	0.3767064647917522
1101	8	0.36650826307999296
1101	9	0.48335854778299137
1101	10-11	0.5094586212939092
1101	12-13	0.47011288595036405
1101	14-15	0.5803163848091302
1101	16-17	0.5914604766180389
1101	18-19	0.6267675209585803
1101	20-21	0.5700927347290872
1101	22-23	0.5199251741690105
1101	24-25	0.5910140646276361
1101	26-27	0.5898453633040788
1101	28-29	0.6613696322062594
1101	30-31	0.6615438546228631
1101	32-33	0.6625685364365808
1101	34-35	0.6426116576456451
1101	36-37	0.7556561446776158
1101	38-39	0.7990071368500296
1101	40-41	0.7657447607285022
1101	42-43	0.6515318114576019
1101	44-45	0.7024842365200996
1101	46-47	0.6700456137683375
1101	48-49	0.761406885302641
1101	50-51	0.6984946689900937
1101	52-53	0.7379496997606623
1101	54-55	0.7324375268215775
1101	56-57	0.7184962187398654
1101	58-59	0.6750184230577077
1101	60-61	0.6824858193623982
1101	62-63	0.6175326716618699
1101	64-65	0.6620088579945786
1101	66-67	0.7146000227152172
1101	68-69	0.6942505654550502
1101	70-71	0.6063105994772542
1101	72-73	0.55509237973137
1101	74-75	0.543201881973367
1101	76-77	0.603042089494096
1101	78-79	0.5047537940363469
1101	80-81	0.5065829769332169
1101	82-83	0.4908540938317998
1101	84-85	0.4698984366503325
1101	86-87	0.511787462117347
1101	88-89	0.5117834341029521
1101	90-91	0.506714799077848
1101	92-93	0.5779862785241718
1101	94-95	0.5283835291580701
1101	96-97	0.510649836384026
1101	98-99	0.537094014731565
1101	100	0.5624798424508413
1102	1	0.4501926789048092
1102	2	0.5543789770053245
1102	3	0.43812545161500793
1102	4	0.347099018415868
1102	5	0.33654814482940765
1102	6	0.35056806974919397
1102	7	0.3523552330193027
1102	8	0.35849417542276285
1102	9	0.47301766854048566
1102	10-11	0.41775579117669537
1102	12-13	0.4863216972565354
1102	14-15	0.5159112407561182
1102	16-17	0.5647244997338419
1102	18-19	0.5504690031937756
1102	20-21	0.5839073003454089
1102	22-23	0.5543404933411793
1102	24-25	0.5909975386220268
1102	26-27	0.6716093386419786
1102	28-29	0.6532603887587882
1102	30-31	0.6947414496965223
1102	32-33	0.6191490693620096
1102	34-35	0.6829733636554494
1102	36-37	0.6836671391466353
1102	38-39	0.7026836005628425
1102	40-41	0.6662462562445057
1102	42-43	0.7086359862466338
1102	44-45	0.7834588888276954
1102	46-47	0.7619813080553399
1102	48-49	0.7455448450974984
1102	50-51	0.6934712383143378
1102	52-53	0.6508303985511787
1102	54-55	0.7005085589438806
1102	56-57	0.7209973209319358
1102	58-59	0.6977740577380729
1102	60-61	0.7275980894440295
1102	62-63	0.6047761454272447
1102	64-65	0.6928338586958702
1102	66-67	0.6432304298754943
1102	68-69	0.6508109471542838
1102	70-71	0.5929786632289336
1102	72-73	0.6195757035416278
1102	74-75	0.6227584229888876
1102	76-77	0.5551056975646489
1102	78-79	0.5301421889201734
1102	80-81	0.47358574405159004
1102	82-83	0.5277054112344643
1102	84-85	0.5766909849768993
1102	86-87	0.5967173801028522
1102	88-89	0.5783237580816589
1102	90-91	0.5394339400375756
1102	92-93	0.5429692977409033
1102	94-95	0.5742289938545966
1102	96-97	0.5696099241053574
1102	98-99	0.5588414130607546
1102	100	0.5335786338122972
1103	1	0.2721112333003113
1103	2	-0.15596721159933935
1103	3	0.16931912833329932
1103	4	0.17863197292904687
1103	5	0.19243686113010483
1103	6	0.24612133869329966
1103	7	0.3539388206835383
1103	8	0.3064105553527199
1103	9	0.3791091577580943
1103	10-11	0.3995752639307071
1103	12-13	0.39174912246649995
1103	14-15	0.5383745766892005
1103	16-17	0.5305415518838856
1103	18-19	0.5167829199917051
1103	20-21	0.46207991911994384
1103	22-23	0.4325118013193432
1103	24-25	0.44607319706053516
1103	26-27	0.5037275637362981
1103	28-29	0.5247209290544959
1103	30-31	0.5785949871354177
1103	32-33	0.6512930734516971
1103	34-35	0.5396887555362326
1103	36-37	0.5300411486761192
1103	38-39	0.48732485380069335
1103	40-41	0.5557976798786015
1103	42-43	0.5433032005348934
1103	44-45	0.5101660008447126
1103	46-47	0.48357442083989355
1103	48-49	0.4271591782189219
1103	50-51	0.5656006488710545
1103	52-53	0.5315717412872942
1103	54-55	0.47199793675981283
1103	56-57	0.4973361355787773
1103	58-59	0.4391137353258614
1103	60-61	0.42922660829511017
1103	62-63	0.45911440808315973
1103	64-65	0.4019600599861235
1103	66-67	0.47750455089549604
1103	68-69	0.40772230309302415
1103	70-71	0.37808881745868916
1103	72-73	0.3416443441219883
1103	74-75	0.39963604776760064
1103	76-77	0.3244090311382948
1103	78-79	0.3323701786464426
1103	80-81	0.3529513331708216
1103	82-83	0.3593683530547622
1103	84-85	0.3823339633684171
1103	86-87	0.374378569825339
1103	88-89	0.38822995603667465
1103	90-91	0.30815498873293734
1103	92-93	0.34035891958343
1103	94-95	0.33847542066009595
1103	96-97	0.3022475886740281
1103	98-99	0.36912431932934453
1103	100	0.4003881772837694
1104	1	0.37552672988069347
1104	2	0.4578333266251171
1104	3	0.34745755040242443
1104	4	0.22742638154420547
1104	5	0.2886944833010361
1104	6	0.2665195910457143
1104	7	0.31114034409826985
1104	8	0.3188915695001526
1104	9	0.354794986162311
1104	10-11	0.32747556749342266
1104	12-13	0.3522978742455649
1104	14-15	0.49574462627681015
1104	16-17	0.47829579005582445
1104	18-19	0.48238054342347425
1104	20-21	0.4930792903399066
1104	22-23	0.45724819062206734
1104	24-25	0.47910221773152983
1104	26-27	0.4861655007097099
1104	28-29	0.5436091484633394
1104	30-31	0.4657571643407721
1104	32-33	0.4555887448999485
1104	34-35	0.4702764082462352
1104	36-37	0.4787184021893083
1104	38-39	0.5025967326155794
1104	40-41	0.5034737365302036
1104	42-43	0.49894922573344047
1104	44-45	0.5594169370426414
1104	46-47	0.524782761233233
1104	48-49	0.5525738672048206
1104	50-51	0.5304492905257305
1104	52-53	0.5290234361928157
1104	54-55	0.5255995948829977
1104	56-57	0.5384145952809618
1104	58-59	0.5327050770505863
1104	60-61	0.567606024431953
1104	62-63	0.5069444373332672
1104	64-65	0.5181060906920578
1104	66-67	0.4714246955969301
1104	68-69	0.45180756407189904
1104	70-71	0.3684876299245161
1104	72-73	0.34143956290954947
1104	74-75	0.33402187872631117
1104	76-77	0.3229907255540141
1104	78-79	0.4149729858223594
1104	80-81	0.4420834222086967
1104	82-83	0.36452371656251614
1104	84-85	0.37478265030087954
1104	86-87	0.39592589835388026
1104	88-89	0.36294346037099245
1104	90-91	0.33707744067977785
1104	92-93	0.38327284046400933
1104	94-95	0.3882816190766434
1104	96-97	0.4752529580289746
1104	98-99	0.4240711636293
1104	100	0.42427919374430445
1105	1	0.11232977190445226
1105	2	-0.33252409741702493
1105	3	0.02179735696883256
1105	4	0.08436223496354955
1105	5	0.04802529498240915
1105	6	0.1913734268494025
1105	7	0.21588441370690248
1105	8	0.19637436521001206
1105	9	0.23908320575848307
1105	10-11	0.2723055627101232
1105	12-13	0.25955113507937
1105	14-15	0.3216114005736799
1105	16-17	0.3884632160396464
1105	18-19	0.3487136061057967
1105	20-21	0.3543912970039713
1105	22-23	0.30415279337451295
1105	24-25	0.3117953031640113
1105	26-27	0.3449341804154713
1105	28-29	0.3816049455191006
1105	30-31	0.3857909391422254
1105	32-33	0.45368070064495214
1105	34-35	0.4343497188150991
1105	36-37	0.42471817885090957
1105	38-39	0.4453509888044067
1105	40-41	0.4495712893896311
1105	42-43	0.46057280809195333
1105	44-45	0.4979919769086436
1105	46-47	0.4156127479518119
1105	48-49	0.44216940555468653
1105	50-51	0.49328672045736255
1105	52-53	0.4656391804618494
1105	54-55	0.44534245207632495
1105	56-57	0.458307267761775
1105	58-59	0.4512070645380035
1105	60-61	0.4048214977253224
1105	62-63	0.3627221106827605
1105	64-65	0.3557725614721363
1105	66-67	0.403627954743051
1105	68-69	0.3841122517005715
1105	70-71	0.40185499873219044
1105	72-73	0.3972396226160484
1105	74-75	0.3174576216461418
1105	76-77	0.28066053065520435
1105	78-79	0.25084033636860426
1105	80-81	0.34734485107891544
1105	82-83	0.34362971173777623
1105	84-85	0.3223816741239389
1105	86-87	0.3608890057425569
1105	88-89	0.3447566101524302
1105	90-91	0.3162299368397399
1105	92-93	0.300774627099635
1105	94-95	0.24441296726670458
1105	96-97	0.29581059936536036
1105	98-99	0.3398816321085789
1105	100	0.3552518716467361
1106	1	0.30319514377505996
1106	2	0.3638638011194608
1106	3	0.26959790502071
1106	4	0.21938239102522772
1106	5	0.2147000265984289
1106	6	0.22313950909477143
1106	7	0.21141418760640107
1106	8	0.19400523374054046
1106	9	0.22736237980107177
1106	10-11	0.24370535880014899
1106	12-13	0.26989341573764847
1106	14-15	0.34517641114857867
1106	16-17	0.30004149577051464
1106	18-19	0.35033017260580124
1106	20-21	0.35323197806720685
1106	22-23	0.3145064350918716
1106	24-25	0.31293126881265465
1106	26-27	0.34041268928908863
1106	28-29	0.30321195470346396
1106	30-31	0.34196439137676293
1106	32-33	0.3559767145823045
1106	34-35	0.256525931085676
1106	36-37	0.28315840081513954
1106	38-39	0.333107091271053
1106	40-41	0.29684064481222805
1106	42-43	0.39228568972360733
1106	44-45	0.32337845980105584
1106	46-47	0.16036201007907636
1106	48-49	0.21836783248281222
1106	50-51	0.18579657056572074
1106	52-53	0.19799990895539565
1106	54-55	0.21756212390442187
1106	56-57	0.3372588299564896
1106	58-59	0.2849685414869043
1106	60-61	0.21616451937711645
1106	62-63	0.2798044027610018
1106	64-65	0.20712186957662482
1106	66-67	0.29477238788721394
1106	68-69	0.32853430130015226
1106	70-71	0.3600862052612257
1106	72-73	0.24298245845141508
1106	74-75	0.1809810058615895
1106	76-77	0.2095665995225815
1106	78-79	0.28188288746216017
1106	80-81	0.2678256426838601
1106	82-83	0.29283518799266517
1106	84-85	0.2536225808935484
1106	86-87	0.169343583316639
1106	88-89	0.2652251991383494
1106	90-91	0.2548342826271792
1106	92-93	0.18292727288692134
1106	94-95	0.17606462541327517
1106	96-97	0.11183381632076106
1106	98-99	0.21568092118680227
1106	100	0.2649182674713657
1107	1	0.048998351591624356
1107	2	-0.4996256242028103
1107	3	-0.029622133941639817
1107	4	0.007004168966929569
1107	5	0.041788005145242835
1107	6	0.1755633230165472
1107	7	0.1330198278739303
1107	8	0.12143343941729512
1107	9	0.11215362474254675
1107	10-11	0.19054853144972128
1107	12-13	0.09097645318249903
1107	14-15	0.22108308312838432
1107	16-17	0.23051053977951597
1107	18-19	0.23275055642732667
1107	20-21	0.18637739738208126
1107	22-23	0.19214683187766468
1107	24-25	0.13276738197355797
1107	26-27	0.13784757940458547
1107	28-29	0.10430175073653913
1107	30-31	0.19185436635726205
1107	32-33	0.20347957963601004
1107	34-35	0.21115571506626196
1107	36-37	0.2524618185845995
1107	38-39	0.23997604780197435
1107	40-41	0.25628707862937006
1107	42-43	0.22264107447292503
1107	44-45	0.16987749187565981
1107	46-47	0.2676602738185352
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2216	74-75	-0.7282889680178855
2216	76-77	-0.6425081116815718
2216	78-79	-0.5985183666100085
2216	80-81	-0.569765745989379
2216	82-83	-0.49290064611541595
2216	84-85	-0.5275906908367816
2216	86-87	-0.3328381462013894
2216	88-89	-0.4096242533812742
2216	90-91	-0.5679277796053057
2216	92-93	-0.513859734604786
2216	94-95	-0.44314598737829414
2216	96-97	-0.528985803949368
2216	98-99	-0.4558337914074144
2216	100	-0.39483295722927636
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	7.0
13	66.0
14	253.0
15	772.0
16	1836.0
17	3533.0
18	5966.0
19	8765.0
20	12187.0
21	16270.0
22	21828.0
23	24734.0
24	25575.0
25	27479.0
26	31342.0
27	38038.0
28	47213.0
29	59104.0
30	72238.0
31	88786.0
32	110786.0
33	139247.0
34	186113.0
35	254233.0
36	374924.0
37	627990.0
38	600562.0
39	211226.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72898563613641	20.89761275408913	13.37395326879703	24.99944834097743
2	14.952793935104658	21.335215714382663	39.63818434251668	24.073806007995994
3	17.53952019159441	27.84477349289728	30.343269975552587	24.272436339955718
4	12.17090677818179	17.971046283546325	38.86779291946742	30.99025401880447
5	12.499924782018288	38.911197985094134	35.09727857999148	13.491598652896093
6	28.650775476911193	39.04011018307573	18.69618922338649	13.61292511662658
7	25.128394390661935	32.91275666759935	24.738606303668988	17.220242638069728
8	25.597674655719675	34.472426651868616	21.025511594127806	18.904387098283905
9	26.71541777470971	14.518057688996903	21.491349324811083	37.275175211482306
10-11	24.895357835918812	26.010844475050853	28.247166446311656	20.846631242718676
12-13	25.801593576219712	24.139604230147572	28.301221674909783	21.757580518722932
14-15	23.07095797537741	24.804374822704105	26.493042478633804	25.631624723284673
16-17	21.729554103119817	27.147254184388913	27.566286463697853	23.556905248793417
18-19	21.440486597345377	26.69079707225917	30.057916316355133	21.810800014040318
20-21	22.663998558526274	25.94328442730336	29.290381739817335	22.10233527435303
22-23	22.910903888349093	25.70232698543923	29.068873199627127	22.31789592658455
24-25	22.45825930129355	25.96297367273019	29.598680878589683	21.98008614738658
26-27	22.279600050812668	26.22027291751633	29.03743422768087	22.46269280399013
28-29	21.88735318255591	26.57051833849538	29.164567583243212	22.3775608957055
30-31	22.535865493742865	26.26793274687143	28.964292459847197	22.231909299538508
32-33	22.722334303341093	26.528982394442707	28.402947129841195	22.345736172375002
34-35	22.76336363993451	26.217747873258844	29.03837619687953	21.980512289927116
36-37	22.7483924670345	26.02881274058672	28.853106504177834	22.36968828820095
38-39	22.821418841105373	25.879885069959236	29.252954735014047	22.045741353921343
40-41	22.47711796797159	25.56618184510771	29.45773608161195	22.498964105308744
42-43	22.51154573246662	25.716186423254932	28.778049485769873	22.994218358508576
44-45	22.478701337340397	26.526826202578402	28.543316891673925	22.451155568407277
46-47	22.693718795808632	26.197564004633307	27.990044745159853	23.118672454398215
48-49	23.239412136166234	25.873403283375225	28.01148502710962	22.875699553348912
50-51	22.973204719552985	26.186047600024736	27.397877571760237	23.442870108662042
52-53	22.428774631149118	26.57367741029657	27.495474504284807	23.502073454269514
54-55	22.52774685090521	26.60282869870399	27.30834026019134	23.56108419019946
56-57	23.133788997861192	26.088025534685976	27.305255564150617	23.47292990330222
58-59	22.40840347933061	26.116090232601675	28.097583723900005	23.377922564167708
60-61	22.569815127286464	26.785424465109358	27.348057294578677	23.2967031130255
62-63	22.815485134812135	26.207960526645685	27.147308019529415	23.829246319012764
64-65	22.047663540467042	26.24715641751229	27.493117736433128	24.212062305587544
66-67	22.624820944144602	25.914718433100386	27.39654039851106	24.063920224243954
68-69	22.210356510138997	25.95653026372246	27.599369581347254	24.233743644791282
70-71	22.217820663446396	25.672381282856033	27.718504381528895	24.391293672168672
72-73	22.625121808125666	25.84087304041439	27.762792151461614	23.77121299999833
74-75	22.56575560443675	26.31842159805842	27.38878042909961	23.72704236840522
76-77	22.300142596792735	26.062200684217224	27.363440295135742	24.2742164238543
78-79	22.034690131058703	26.209617367563588	27.379332442882763	24.376360058494946
80-81	21.876832403411235	26.4125343915167	27.72624854159911	23.984384663472948
82-83	22.38070412409716	25.59241555647339	28.291496083602848	23.735384235826597
84-85	22.629039941829852	26.005766868089157	27.172629942582056	24.192563247498935
86-87	21.586140862737548	26.502489173907186	27.557299810474422	24.354070152880848
88-89	22.153514356355622	25.669985271632324	27.398458093188726	24.778042278823328
90-91	21.898766550604186	25.561822075050024	27.64302346378801	24.896387910557785
92-93	22.043531287109648	25.856764787692704	27.386233858975316	24.71347006622233
94-95	21.162706345651113	25.76464059585336	27.829517874932304	25.24313518356322
96-97	21.40558535355764	25.548465993503722	27.607871219141682	25.438077433796952
98-99	20.99301446916852	26.200571251679023	27.45459705829455	25.35181722085791
100	21.4858867358905	25.584446217698755	27.00147715429487	25.928189892115878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	354.0
1	278.0
2	298.0
3	374.0
4	270.5
5	233.5
6	410.5
7	947.5
8	1606.5
9	1733.0
10	1321.0
11	982.0
12	921.5
13	925.5
14	1062.0
15	1268.0
16	1440.0
17	1734.5
18	2147.0
19	2578.5
20	3098.5
21	3767.5
22	4466.0
23	5193.0
24	5914.5
25	6942.5
26	8621.0
27	10455.0
28	12785.0
29	17809.5
30	25265.0
31	32821.5
32	42011.5
33	51137.5
34	60297.0
35	68879.0
36	77596.0
37	92445.5
38	98122.0
39	91935.0
40	86623.5
41	82871.0
42	83566.0
43	88322.0
44	94577.0
45	106899.5
46	118997.5
47	127159.0
48	132283.0
49	129067.0
50	123529.5
51	122965.5
52	121818.0
53	118631.5
54	115887.5
55	105365.0
56	94165.0
57	80302.0
58	66687.0
59	60847.0
60	50377.5
61	38117.0
62	28991.0
63	22281.5
64	16224.5
65	10313.0
66	6845.0
67	4920.0
68	3602.5
69	2578.0
70	1903.5
71	1406.0
72	985.0
73	730.0
74	523.0
75	335.5
76	196.0
77	105.5
78	60.0
79	27.5
80	13.5
81	5.5
82	5.0
83	2.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013605737810412234
2	0.0046132477096729445
3	0.0022063358611479297
4	0.02737862227697204
5	0.002640917167131613
6	0.0
7	5.348692996722254E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009009204766354046
16-17	0.0029417811481972397
18-19	0.0
20-21	6.685866245902817E-5
22-23	8.524479463526092E-4
24-25	0.0
26-27	1.6714665614757046E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.178666403689261E-4
36-37	0.0
38-39	0.0
40-41	3.1757864668038383E-4
42-43	6.685866245902818E-4
44-45	0.0
46-47	0.0
48-49	4.0115197475416907E-4
50-51	0.0
52-53	0.0
54-55	3.1757864668038383E-4
56-57	1.0028799368854227E-4
58-59	1.6714665614757046E-4
60-61	8.357332807378523E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013204585835658064
70-71	6.853012902050389E-4
72-73	0.0
74-75	1.6714665614757046E-4
76-77	0.0027913491576644265
78-79	5.181546340574684E-4
80-81	0.0031757864668038385
82-83	0.0017216105583199755
84-85	0.005348692996722255
86-87	0.007755604845247268
88-89	0.007304308873648828
90-91	0.006017279621312536
92-93	0.008323903476149008
94-95	0.001504319905328134
96-97	0.004446101053525373
98-99	0.001972330542541331
100	0.08457620801067064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2991385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.90741864154715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99269952602123	25.479867201314832
2	14.28028260167666	9.969756061926587
3	4.991100174178838	5.226792697858788
4	2.2890841246967635	3.196240713860378
5	1.2583087938572848	2.1962155923758244
6	0.7940719757130112	1.663140173264073
7	0.5324723569844688	1.3011064836215736
8	0.3828311819282348	1.0690918669285752
9	0.29180156376045974	0.9167435411800035
>10	1.7438002165755553	11.585893783621781
>50	0.18564500881955	4.57351719153112
>100	0.21160857534806274	16.103824476837026
>500	0.03230029906466062	7.545685445536714
>1k	0.013322675281862812	7.624478019581062
>5k	6.709260933312206E-4	1.5476467505617357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7868	0.26302197811381683	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7471	0.24975053361569974	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6884	0.23012751618397498	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6708	0.22424395388758048	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6047	0.2021471659448717	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5781	0.19325496383782095	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	5498	0.18379446309986844	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4573	0.15287233171256792	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4440	0.14842623065904254	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	4141	0.13843086062141782	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	4035	0.13488735151108933	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	3526	0.11787182191526667	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	3124	0.10443323076100201	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3013	0.10072257499452594	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	3.342933122951409E-5	0.0	0.0	8.357332807378521E-5
14-15	0.0	3.342933122951409E-5	0.0	0.0	1.6714665614757043E-4
16-17	0.0	3.342933122951409E-5	0.0	0.0	2.674346498361127E-4
18-19	0.0	3.342933122951409E-5	0.0	1.6714665614757044E-5	3.008639810656268E-4
20-21	0.0	3.342933122951409E-5	0.0	3.342933122951409E-5	3.8443730913941205E-4
22-23	0.0	3.342933122951409E-5	0.0	6.685866245902817E-5	4.6801063721319723E-4
24-25	0.0	3.342933122951409E-5	0.0	1.6714665614757043E-4	4.6801063721319723E-4
26-27	0.0	3.342933122951409E-5	0.0	9.861652712706654E-4	4.847253028279543E-4
28-29	0.0	3.342933122951409E-5	0.0	0.0027579198264349124	5.014399684427113E-4
30-31	0.0	3.342933122951409E-5	0.0	0.0119844152457808	5.014399684427113E-4
32-33	0.0	3.342933122951409E-5	0.0	0.03399762986041583	5.014399684427113E-4
34-35	0.0	3.342933122951409E-5	0.0	0.06916528631386465	5.682986309017394E-4
36-37	0.0	3.342933122951409E-5	0.0	0.12392253086780872	5.682986309017394E-4
38-39	0.0	3.342933122951409E-5	0.0	0.21180824267020126	6.518719589755247E-4
40-41	0.0	3.342933122951409E-5	0.0	0.3183308066330479	6.685866245902817E-4
42-43	0.0	3.342933122951409E-5	0.0	0.42697613312896865	6.685866245902817E-4
44-45	0.0	3.342933122951409E-5	0.0	0.5577182475675984	6.685866245902817E-4
46-47	0.0	3.342933122951409E-5	0.0	0.7158891282800441	7.187306214345529E-4
48-49	0.0	3.342933122951409E-5	0.0	0.8968922422222483	7.354452870493099E-4
50-51	0.0	3.342933122951409E-5	0.0	1.1007777333910547	8.691626119673663E-4
52-53	0.0	3.342933122951409E-5	0.0	1.3055491018374432	8.691626119673663E-4
54-55	0.0	3.342933122951409E-5	0.0	1.519463392375104	8.691626119673663E-4
56-57	0.0	3.342933122951409E-5	0.0	1.771186256533345	8.691626119673663E-4
58-59	0.0	8.357332807378521E-5	0.0	2.070696349684176	8.858772775821233E-4
60-61	0.0	1.504319905328134E-4	0.0	2.407179283174851	9.527359400411515E-4
62-63	0.0	1.6714665614757043E-4	0.0	2.7580535437598304	0.0011031679305739648
64-65	0.0	1.6714665614757043E-4	0.0	3.117669574461328	0.0011031679305739648
66-67	0.0	1.6714665614757043E-4	0.0	3.514041154849677	0.0011198825961887219
68-69	0.0	1.6714665614757043E-4	0.0	3.961459324025493	0.001136597261803479
70-71	0.0	1.6714665614757043E-4	0.0	4.470303889335542	0.0012034559242625072
72-73	0.0	1.6714665614757043E-4	0.0	5.020951833348098	0.0013371732491805634
74-75	0.0	1.6714665614757043E-4	0.0	5.557024588944586	0.0013873172460248347
76-77	0.0	1.6714665614757043E-4	0.0	6.1274961263762435	0.0014374612428691058
78-79	0.0	1.6714665614757043E-4	0.0	6.749014252595369	0.0014374612428691058
80-81	0.0	1.6714665614757043E-4	0.0	7.446182955386886	0.0014708905740986199
82-83	0.0	1.6714665614757043E-4	0.0	8.19200804978296	0.0016380372302461903
84-85	0.0	1.6714665614757043E-4	0.0	8.919229721349808	0.0016380372302461903
86-87	0.0	1.6714665614757043E-4	0.0	9.669032906162196	0.0016881812270904614
88	0.0	1.6714665614757043E-4	0.0	10.244284837959674	0.0017383252239347325
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5355	0.0	48.820072	1
GTATCAA	10535	0.0	45.30171	1
CAACGCA	13310	0.0	35.16791	5
ATCAACG	13445	0.0	34.78798	3
TATCAAC	13790	0.0	34.43012	2
AACGCAG	14690	0.0	32.472034	6
TCAACGC	15190	0.0	30.977276	4
ACGCAGA	15915	0.0	29.293432	7
CGCAGAG	16160	0.0	28.791157	8
GGGCACC	2295	0.0	27.255474	94
GTACATG	12600	0.0	27.01787	1
TAGTACT	2455	0.0	26.806705	4
TATTCTC	1815	0.0	26.670176	5
TACATGG	12755	0.0	26.27789	2
GCAGAGT	17435	0.0	26.177921	9
ACATGGG	12665	0.0	26.018349	3
CTGTGCG	2495	0.0	25.998386	9
CTAGTAC	2900	0.0	24.6384	3
CATGGGG	9620	0.0	24.187838	4
GTACTGT	3010	0.0	23.1079	6
>>END_MODULE