##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146455_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5281626 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.072383769695165 31.0 30.0 34.0 26.0 34.0 2 30.20602386462048 31.0 30.0 34.0 26.0 34.0 3 30.36414808621436 31.0 30.0 34.0 26.0 34.0 4 33.86853328880159 35.0 35.0 37.0 28.0 37.0 5 34.054570126699616 37.0 35.0 37.0 28.0 37.0 6 34.220291440552586 37.0 35.0 37.0 30.0 37.0 7 34.089021259740846 37.0 35.0 37.0 29.0 37.0 8 34.009707616555964 37.0 35.0 37.0 29.0 37.0 9 35.35380486993967 39.0 35.0 39.0 28.0 39.0 10-11 35.268408535553256 38.5 35.0 39.0 27.5 39.0 12-13 35.24729988454313 38.5 35.0 39.0 27.0 39.0 14-15 36.203031036275576 39.0 35.5 41.0 27.0 41.0 16-17 36.03434927047087 39.0 35.0 41.0 27.0 41.0 18-19 35.981117178687015 39.0 35.0 41.0 27.0 41.0 20-21 36.313109826405736 39.0 35.0 41.0 27.0 41.0 22-23 36.02216154646315 39.0 34.5 41.0 27.0 41.0 24-25 36.1165468740119 39.0 35.0 41.0 27.0 41.0 26-27 36.17349354157224 39.0 35.0 41.0 27.0 41.0 28-29 36.01108427594078 39.0 35.0 41.0 27.0 41.0 30-31 35.946874882848576 39.0 34.5 41.0 27.0 41.0 32-33 36.048249629943506 39.0 35.0 41.0 27.0 41.0 34-35 36.091063907213424 39.0 35.0 41.0 27.5 41.0 36-37 36.01149201022564 39.0 35.0 41.0 27.0 41.0 38-39 35.84486283201423 39.0 34.0 41.0 27.0 41.0 40-41 35.80047716366134 39.0 34.0 41.0 27.0 41.0 42-43 35.63726473627629 38.5 34.0 41.0 27.0 41.0 44-45 35.447935825066 38.0 34.0 40.0 26.0 41.0 46-47 35.31579574169015 38.0 33.0 40.0 26.0 41.0 48-49 35.17918695871309 38.0 33.0 40.0 26.0 41.0 50-51 34.959851189766184 38.0 33.0 40.0 25.5 41.0 52-53 34.76639542443937 37.5 33.0 40.0 25.0 41.0 54-55 34.568886172553675 37.0 33.0 40.0 25.0 41.0 56-57 34.40849238473152 37.0 33.0 40.0 25.0 41.0 58-59 34.13956364952763 36.0 32.0 40.0 25.0 41.0 60-61 33.884283836076236 36.0 32.0 39.0 24.5 41.0 62-63 33.610647649038384 35.0 32.0 39.0 24.0 41.0 64-65 33.32279519602486 35.0 31.5 39.0 23.5 41.0 66-67 33.03439499502615 35.0 31.0 38.0 23.0 40.0 68-69 32.74880519749032 35.0 31.0 37.5 23.0 40.0 70-71 32.70289130657869 35.0 31.0 37.0 23.5 40.0 72-73 32.50650632967954 35.0 31.0 36.5 23.0 40.0 74-75 32.2543484714745 35.0 31.0 36.0 23.0 39.0 76-77 32.03460515757837 35.0 31.0 36.0 23.0 39.0 78-79 31.82972762554562 35.0 30.5 35.5 23.0 39.0 80-81 31.6318247448797 34.0 30.0 35.0 22.5 37.5 82-83 31.406334621194304 34.0 30.0 35.0 22.0 37.0 84-85 31.232977495945377 34.0 30.0 35.0 21.5 36.5 86-87 31.079941290807035 34.0 30.0 35.0 21.0 36.0 88-89 31.103290539693646 34.0 30.0 35.0 21.0 36.0 90-91 31.035664774446353 34.0 30.0 35.0 21.0 36.0 92-93 30.897775041246767 34.0 30.0 35.0 20.0 35.5 94-95 30.87888777054642 34.0 30.0 35.0 20.0 35.0 96-97 30.79895869188769 34.0 30.0 35.0 20.0 35.0 98-99 30.727434695300275 34.0 30.0 35.0 20.0 35.0 100 30.21201065732409 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8089858932173009 1101 2 0.9965931718778336 1101 3 1.0150220203095621 1101 4 1.0388236756492049 1101 5 0.997223364717513 1101 6 0.7533291182127186 1101 7 0.7141009647470042 1101 8 0.7890961470039102 1101 9 1.0148732640558435 1101 10-11 1.005940864238255 1101 12-13 0.8469423490013881 1101 14-15 1.1600770802240774 1101 16-17 1.2184296339010103 1101 18-19 1.3858500392873836 1101 20-21 1.0095333705256948 1101 22-23 1.394795928883731 1101 24-25 1.2323575210326112 1101 26-27 1.1852804432352855 1101 28-29 1.3838149916472346 1101 30-31 1.3844905708150321 1101 32-33 1.1840288867463897 1101 34-35 1.1135179736947478 1101 36-37 1.0666962905476325 1101 38-39 1.2157555884602331 1101 40-41 1.1021419242912742 1101 42-43 1.1411086421734211 1101 44-45 1.151463660780216 1101 46-47 1.2158697268069005 1101 48-49 1.186988623357827 1101 50-51 1.174720714951583 1101 52-53 1.2109740997445613 1101 54-55 1.1964527764170754 1101 56-57 1.0392391315140017 1101 58-59 0.9534241591374979 1101 60-61 0.9517118799650461 1101 62-63 0.9337481703174362 1101 64-65 0.8785751939911677 1101 66-67 0.9503827319797082 1101 68-69 1.03079100434433 1101 70-71 0.9024662005152209 1101 72-73 0.853494213892148 1101 74-75 0.8192394506658758 1101 76-77 0.8237418108272117 1101 78-79 0.9078069943090838 1101 80-81 0.8895211758005601 1101 82-83 0.921542397738417 1101 84-85 0.9099819955615764 1101 86-87 0.9223801220494998 1101 88-89 0.8884790981525335 1101 90-91 0.9105144316610776 1101 92-93 0.9050087068925841 1101 94-95 0.8473192128188529 1101 96-97 0.8059103267116647 1101 98-99 0.9170348044425651 1101 100 0.9833245519188232 1102 1 0.8130426656493839 1102 2 0.9418762321538878 1102 3 0.9914731546205893 1102 4 1.0214867044565423 1102 5 0.9499468498871693 1102 6 0.7174945673493696 1102 7 0.8036102371168639 1102 8 0.7783042947806607 1102 9 1.0026919055335881 1102 10-11 0.919477996820703 1102 12-13 0.8679496361016348 1102 14-15 1.1399831788854229 1102 16-17 1.3230536639544823 1102 18-19 1.3138822866923192 1102 20-21 1.0600272591322195 1102 22-23 1.3481245984903296 1102 24-25 1.2080803905075612 1102 26-27 1.0550423276923766 1102 28-29 1.2594061260900489 1102 30-31 1.288209555248784 1102 32-33 1.1372141089081111 1102 34-35 1.050870669989365 1102 36-37 0.9562364075557639 1102 38-39 1.071924559679907 1102 40-41 0.9563506422363872 1102 42-43 0.9994976145726397 1102 44-45 1.0476011553841715 1102 46-47 0.9764261259066771 1102 48-49 0.9154866798025338 1102 50-51 1.0786376041717887 1102 52-53 0.9708395509015801 1102 54-55 0.9886935391858103 1102 56-57 0.8317078322930698 1102 58-59 0.7818614643333746 1102 60-61 0.8406469895225968 1102 62-63 0.8724833955641671 1102 64-65 0.7570717024859448 1102 66-67 0.8314313361372996 1102 68-69 0.8597640404451354 1102 70-71 0.8012162928884834 1102 72-73 0.7431000472972329 1102 74-75 0.717963553272881 1102 76-77 0.7556975258992189 1102 78-79 0.7395519975893166 1102 80-81 0.871578741077073 1102 82-83 0.8538253129401134 1102 84-85 0.8176859436153698 1102 86-87 0.8082803920326604 1102 88-89 0.7858909149708886 1102 90-91 0.8115208550678901 1102 92-93 0.8959164546856044 1102 94-95 0.9040274669235622 1102 96-97 0.8132155408333048 1102 98-99 0.8788437074228348 1102 100 0.8878761891052811 1103 1 0.6866560239396229 1103 2 0.8871895858616448 1103 3 0.8209540549828063 1103 4 0.8730964652459008 1103 5 0.8339448194947323 1103 6 0.6424376514006696 1103 7 0.7223721308002382 1103 8 0.7811007423996088 1103 9 0.8316644524322854 1103 10-11 0.9492712487872623 1103 12-13 0.814498623599512 1103 14-15 0.9469097025484174 1103 16-17 1.2213241795039522 1103 18-19 1.326188456476828 1103 20-21 1.0406426639291269 1103 22-23 1.233029496862443 1103 24-25 1.1531505830675286 1103 26-27 1.0164801673185622 1103 28-29 1.2031019514480974 1103 30-31 1.2378538588951784 1103 32-33 1.0583646218188818 1103 34-35 1.0557342706224588 1103 36-37 1.0390257598797135 1103 38-39 1.0869227493473659 1103 40-41 0.9908494998888528 1103 42-43 1.0107955122201133 1103 44-45 1.0087845664938229 1103 46-47 1.051856501624954 1103 48-49 1.043096806686954 1103 50-51 1.0856992229565492 1103 52-53 1.031191102877763 1103 54-55 1.0081830656944675 1103 56-57 0.8915048912454537 1103 58-59 0.9121486299065396 1103 60-61 0.8817041481918224 1103 62-63 0.8700026231086113 1103 64-65 0.8572539694855692 1103 66-67 0.9253069610041749 1103 68-69 0.9208935444341577 1103 70-71 0.8217119513635893 1103 72-73 0.8440114830092753 1103 74-75 0.821115244032832 1103 76-77 0.780901433800377 1103 78-79 0.821062031464443 1103 80-81 0.8652078938263372 1103 82-83 0.7964006960432393 1103 84-85 0.7825203598750292 1103 86-87 0.8087701134227352 1103 88-89 0.8698286576599372 1103 90-91 0.8333300747214061 1103 92-93 0.8438481182830131 1103 94-95 0.8244450972964614 1103 96-97 0.8454060356558522 1103 98-99 0.8362233429694079 1103 100 0.8917582067876246 1104 1 0.67822585761472 1104 2 0.8620889554978568 1104 3 0.8147209397271347 1104 4 0.8706498725737433 1104 5 0.7789968434941272 1104 6 0.5544688115521268 1104 7 0.6428985550860631 1104 8 0.6315059762634903 1104 9 0.745469527891494 1104 10-11 0.7217571171377912 1104 12-13 0.6623107947199571 1104 14-15 0.9196798313335748 1104 16-17 1.081797874564117 1104 18-19 1.0134748542895409 1104 20-21 0.8899981956154832 1104 22-23 1.101993031239509 1104 24-25 0.9872108419398131 1104 26-27 0.8362650190583025 1104 28-29 1.028954461511134 1104 30-31 1.0415921006033457 1104 32-33 0.8651977218850675 1104 34-35 0.7705895907409328 1104 36-37 0.7468152552631935 1104 38-39 0.9438784502880324 1104 40-41 0.7968444656582534 1104 42-43 0.7393295095510979 1104 44-45 0.8737418731804567 1104 46-47 0.8109186591833506 1104 48-49 0.8044211131240431 1104 50-51 0.8711259933457214 1104 52-53 0.8811026783532512 1104 54-55 0.8752641884146897 1104 56-57 0.7711146298777507 1104 58-59 0.8296615680961708 1104 60-61 0.7759934428570574 1104 62-63 0.720551614635049 1104 64-65 0.687885743726369 1104 66-67 0.8664042930789506 1104 68-69 0.8099587398903338 1104 70-71 0.7058405629358049 1104 72-73 0.6511890589838458 1104 74-75 0.6688975786572655 1104 76-77 0.710064167474382 1104 78-79 0.638313493452749 1104 80-81 0.6778184244819769 1104 82-83 0.6079045639380851 1104 84-85 0.6316236375497333 1104 86-87 0.608285134742264 1104 88-89 0.5736227749878893 1104 90-91 0.6121439926330652 1104 92-93 0.6056877823783182 1104 94-95 0.5947366269864851 1104 96-97 0.6395396380601319 1104 98-99 0.6980776540718381 1104 100 0.7546459456152128 1105 1 0.5196894223366648 1105 2 0.6141716704872593 1105 3 0.6235709831338845 1105 4 0.6990742513320427 1105 5 0.6275381353415881 1105 6 0.26914172518343804 1105 7 0.3584233709356113 1105 8 0.43735454837638343 1105 9 0.5039232078634654 1105 10-11 0.5167548241261812 1105 12-13 0.4787725491695838 1105 14-15 0.6567375247787481 1105 16-17 0.7811027697791957 1105 18-19 0.8522107298552726 1105 20-21 0.696059478041569 1105 22-23 0.8794862271353736 1105 24-25 0.7492693377401167 1105 26-27 0.5592328789663057 1105 28-29 0.8567695410663063 1105 30-31 0.8410612995823783 1105 32-33 0.7069307055241865 1105 34-35 0.5857340372100523 1105 36-37 0.5038772005274481 1105 38-39 0.6887282984771375 1105 40-41 0.5686804345624239 1105 42-43 0.5082377707852004 1105 44-45 0.6078461305430878 1105 46-47 0.4967078755834393 1105 48-49 0.5995974601722125 1105 50-51 0.5709125403567015 1105 52-53 0.6364617077396062 1105 54-55 0.6006347239714813 1105 56-57 0.5537312412548587 1105 58-59 0.51710862260407 1105 60-61 0.5592531054010763 1105 62-63 0.4836113302803966 1105 64-65 0.4811708004787363 1105 66-67 0.5534292026913263 1105 68-69 0.4741866182962653 1105 70-71 0.46770451745646824 1105 72-73 0.4410760025041469 1105 74-75 0.5010173664323503 1105 76-77 0.4863334336125327 1105 78-79 0.4940633162052066 1105 80-81 0.5749533270362761 1105 82-83 0.5548067171940154 1105 84-85 0.4940023327266623 1105 86-87 0.5944278383103736 1105 88-89 0.5149136941049228 1105 90-91 0.5659713194430545 1105 92-93 0.6278895456498681 1105 94-95 0.5507728838897243 1105 96-97 0.5664393829244609 1105 98-99 0.6395049337749619 1105 100 0.6687289889734203 1106 1 0.5249744938008973 1106 2 0.5994081162400882 1106 3 0.5684893937630555 1106 4 0.721035967145184 1106 5 0.5967745981495298 1106 6 0.3943821255894093 1106 7 0.41900767093602553 1106 8 0.40407251805106625 1106 9 0.5794823722324622 1106 10-11 0.5308249616583112 1106 12-13 0.4397994558673446 1106 14-15 0.6460948510068576 1106 16-17 0.7963718014474637 1106 18-19 0.7353774183515895 1106 20-21 0.6139821712349729 1106 22-23 0.9260744194311812 1106 24-25 0.6831392496384936 1106 26-27 0.5487637409167974 1106 28-29 0.7552661932685467 1106 30-31 0.7635912636080917 1106 32-33 0.6343279308446768 1106 34-35 0.6356530787449728 1106 36-37 0.5040217958637143 1106 38-39 0.6779975110894441 1106 40-41 0.5860914745202734 1106 42-43 0.5695869257512385 1106 44-45 0.5482748439896952 1106 46-47 0.5273661374574274 1106 48-49 0.514831082505232 1106 50-51 0.5619044654293504 1106 52-53 0.5424401892567303 1106 54-55 0.6157124586214593 1106 56-57 0.5703207113513216 1106 58-59 0.5245352759801847 1106 60-61 0.499775901751228 1106 62-63 0.5482247662677153 1106 64-65 0.5997795020747105 1106 66-67 0.6351676125130368 1106 68-69 0.5352255901504108 1106 70-71 0.5728285780431364 1106 72-73 0.5148471520037532 1106 74-75 0.5682743841095714 1106 76-77 0.5797056121747168 1106 78-79 0.5115397518422213 1106 80-81 0.5563172040247153 1106 82-83 0.5515292984099744 1106 84-85 0.5237869200523484 1106 86-87 0.5903415144149555 1106 88-89 0.5698471039019886 1106 90-91 0.5676413675957548 1106 92-93 0.6017434657197711 1106 94-95 0.5622859277827779 1106 96-97 0.5901196007181895 1106 98-99 0.6434592871112237 1106 100 0.6117235168154203 1107 1 0.5506621932089502 1107 2 0.6888218717044587 1107 3 0.5919805222498766 1107 4 0.719406379224985 1107 5 0.6735952275661958 1107 6 0.44936339065723274 1107 7 0.4685360754604275 1107 8 0.487702411407291 1107 9 0.614959686855336 1107 10-11 0.6473727295955101 1107 12-13 0.5252250734237052 1107 14-15 0.6791615776574318 1107 16-17 0.8211690297247429 1107 18-19 0.9154693510208887 1107 20-21 0.7238809794982686 1107 22-23 0.9887466285194222 1107 24-25 0.7110168604826725 1107 26-27 0.5711222670734486 1107 28-29 0.7779493262954773 1107 30-31 0.7576698824706014 1107 32-33 0.7146114660356488 1107 34-35 0.5487552846960995 1107 36-37 0.6091222465175505 1107 38-39 0.6072317636413445 1107 40-41 0.4794767456589568 1107 42-43 0.6620637928117006 1107 44-45 0.5920577678121361 1107 46-47 0.5247346677362046 1107 48-49 0.49832370176347496 1107 50-51 0.5890712060277821 1107 52-53 0.6355499854321351 1107 54-55 0.5941470599291421 1107 56-57 0.48618114231268095 1107 58-59 0.4977224545781098 1107 60-61 0.5343026774820814 1107 62-63 0.4846830971875917 1107 64-65 0.6104666715704496 1107 66-67 0.5744453748042346 1107 68-69 0.5938809580230782 1107 70-71 0.5159190809090646 1107 72-73 0.4848948269001383 1107 74-75 0.5446998465647965 1107 76-77 0.4615985979561792 1107 78-79 0.4307079411103558 1107 80-81 0.5355834664575454 1107 82-83 0.5347373802445894 1107 84-85 0.494795553353228 1107 86-87 0.5425771585079744 1107 88-89 0.5324586759290533 1107 90-91 0.5190774100927982 1107 92-93 0.6066727623106658 1107 94-95 0.5999507111479403 1107 96-97 0.5701336054962063 1107 98-99 0.5707216767158627 1107 100 0.5852942446845617 1108 1 0.4247792194487303 1108 2 0.5742684651809462 1108 3 0.538147298707635 1108 4 0.6828642919594117 1108 5 0.5602100914747439 1108 6 0.3642767488843148 1108 7 0.2802554738867755 1108 8 0.3521098278102528 1108 9 0.42516117244241514 1108 10-11 0.555056999531331 1108 12-13 0.33551375119528615 1108 14-15 0.560137256454702 1108 16-17 0.7196624745848652 1108 18-19 0.7887030977162368 1108 20-21 0.5035294674390158 1108 22-23 0.7586728384201962 1108 24-25 0.5913898822772268 1108 26-27 0.5712795275264639 1108 28-29 0.6756434512196776 1108 30-31 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-3.4586962760831064 2216 1 -0.9183347137742253 2216 2 -0.8379729581796163 2216 3 -1.0553702962679914 2216 4 -1.0236619940493412 2216 5 -1.1581949502194107 2216 6 -1.0281198240685399 2216 7 -1.2797677945825754 2216 8 -0.9848451783027699 2216 9 -1.2640109420580856 2216 10-11 -1.2723399090244527 2216 12-13 -1.447205585162557 2216 14-15 -1.841145775172805 2216 16-17 -1.6148149519686115 2216 18-19 -3.7343637392298277 2216 20-21 -1.8444438878540623 2216 22-23 -1.3453677125171168 2216 24-25 -1.4567110923425304 2216 26-27 -1.3437459025143994 2216 28-29 -1.5028390786634134 2216 30-31 -1.6695106315234085 2216 32-33 -1.5211971189720117 2216 34-35 -1.7660239292249926 2216 36-37 -1.746760457734716 2216 38-39 -1.962547198814967 2216 40-41 -1.8716875362833534 2216 42-43 -1.8865273757451462 2216 44-45 -1.6694637198535176 2216 46-47 -1.9668987846237869 2216 48-49 -1.913050679004499 2216 50-51 -1.8085075024491033 2216 52-53 -1.8170152696788833 2216 54-55 -1.6716631595996176 2216 56-57 -1.73945254893885 2216 58-59 -1.728527011059228 2216 60-61 -1.7365406096655533 2216 62-63 -1.7483216554967989 2216 64-65 -1.647478358228124 2216 66-67 -1.7069034822883253 2216 68-69 -1.885317246518376 2216 70-71 -1.946537541415978 2216 72-73 -1.814244534882242 2216 74-75 -1.827900865080533 2216 76-77 -1.75353046634336 2216 78-79 -1.9712443685936734 2216 80-81 -1.8615366331225047 2216 82-83 -1.9983729876483913 2216 84-85 -1.740246378399231 2216 86-87 -1.8195808493880214 2216 88-89 -1.8232263141380862 2216 90-91 -1.8468805449955923 2216 92-93 -1.8060925330347217 2216 94-95 -1.8217736457308789 2216 96-97 -1.6730752737252566 2216 98-99 -1.6754592019020897 2216 100 -1.6544761679691433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 21802.0 1 3327.0 2 620.0 3 534.0 4 498.0 5 295.0 6 270.0 7 327.0 8 598.0 9 1081.0 10 2277.0 11 5501.0 12 10278.0 13 14790.0 14 18296.0 15 22088.0 16 25551.0 17 29348.0 18 32891.0 19 36139.0 20 40754.0 21 45849.0 22 51172.0 23 58246.0 24 65165.0 25 72881.0 26 82358.0 27 92095.0 28 107322.0 29 126045.0 30 148093.0 31 175151.0 32 211072.0 33 264901.0 34 356445.0 35 471152.0 36 683174.0 37 990086.0 38 761533.0 39 251445.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16866893867695 21.291500347188823 12.57102741309618 23.968803301038047 2 15.364167854197069 19.747510084895506 40.871674257161935 24.01664780374549 3 18.416558796603862 26.931140470031906 28.777735162444323 25.874565570919906 4 12.11604793469517 16.691867654771126 40.22338302959311 30.968701380940594 5 13.146185463697273 38.536557537748884 35.01211786417534 13.30513913437851 6 29.678014913301904 38.54190349408393 18.299853903445378 13.480227689168784 7 26.7729729575444 31.73948742734653 23.982337679142116 17.50520193596695 8 26.09608298153524 35.02078439319943 19.989648239962932 18.893484385302404 9 26.21428753588898 14.819433168176449 21.687850825156374 37.278428470778195 10-11 25.74940564904647 25.73076139735764 27.54974179757615 20.970091156019745 12-13 25.854288838844408 23.57880878123626 27.545388592821098 23.02151378709824 14-15 23.33996050723338 24.757638340550074 24.86497819180458 27.037422960411973 16-17 22.297294917998418 27.270857576406186 26.279946212617766 24.15190129297763 18-19 21.751489802646358 28.053905083563713 27.821222159705627 22.37338295408431 20-21 23.48038010197672 27.1360655288377 26.60435940208781 22.779194967097773 22-23 24.10024087949556 26.692644613318013 26.357430162070422 22.849684345116007 24-25 23.39837354426578 26.82586082128287 27.134309171244553 22.641456463206797 26-27 23.29710498975681 27.022759755054064 26.112000377548394 23.568134877640734 28-29 22.40673842226184 27.63415398859199 26.712045586355305 23.247062002790866 30-31 23.289023630731226 27.199058710237 26.58796258630115 22.92395507273062 32-33 23.690054555743856 27.51026982307328 25.58123025862093 23.218445362561937 34-35 23.636285768941228 27.21302285919312 26.686164092441782 22.46452727942387 36-37 23.28307506148227 26.84029878535635 26.34284355537516 23.53378259778622 38-39 23.502625340035 26.834404418006557 26.949398375636584 22.713571866321853 40-41 23.046489510106117 25.966123338490966 27.586418267766422 23.400968883636498 42-43 23.072194819324732 26.27379849084238 26.85275086373618 23.801255826096707 44-45 23.155642944132847 27.26104141199397 26.71591729671288 22.867398347160304 46-47 23.134974930183937 26.926107580989772 26.264908673221605 23.674008815604683 48-49 23.826979158191342 26.43314971910687 26.548904238448216 23.19096688425357 50-51 23.440318711873477 26.27920562637851 26.493219530144017 23.78725613160399 52-53 22.82831814646299 27.013858758727334 26.295163400459344 23.862659694350334 54-55 22.828180151266604 26.917042263374075 26.307207411711573 23.947570173647744 56-57 23.94285238452113 26.313474417996023 26.029401758205157 23.714271439277685 58-59 22.851994915197295 26.633162624891877 27.045215514529826 23.46962694538101 60-61 22.903146573206186 27.358859288819886 26.223340357524776 23.514653780449144 62-63 23.36264371217769 26.56320804576941 25.794471959270233 24.279676282782667 64-65 22.428421601620197 26.765590976229998 26.315506464040446 24.490480958109366 66-67 23.28178989314092 26.321862785302454 26.28360467879512 24.112742642761503 68-69 22.54741939656859 26.41152562888653 26.591443428888113 24.449611545656772 70-71 22.611425716775717 26.02324301426161 26.7375085382278 24.62782273073487 72-73 23.21779637658317 26.182799505994613 27.141638608561642 23.457765508860575 74-75 23.345700274977435 26.5239966635716 26.678388326916686 23.45191473453428 76-77 22.999530373241456 26.464252934215615 26.33405165133044 24.202165041212485 78-79 22.84438227793854 26.467472387990583 26.397325647177222 24.29081968689366 80-81 22.451167460517613 26.759356100439195 27.141527524660138 23.647948914383058 82-83 23.31011541580457 25.66148510354133 28.185316610951087 22.84308286970301 84-85 23.53082315019079 26.255952109591053 26.707194611129793 23.506030129088362 86-87 22.071969765316897 26.93142349301782 27.29383375564091 23.702772986024375 88-89 23.23988896417297 25.62528490790762 27.02606975560286 24.108756372316552 90-91 22.794814906774512 25.696222729248426 27.309186936361552 24.19977542761551 92-93 23.17931347445718 25.896616188170498 27.15976070261947 23.764309634752852 94-95 21.931264177097308 25.904158634354204 27.894047086078533 24.270530102469955 96-97 22.269827919808407 25.81557021215053 27.388683116523378 24.52591875151769 98-99 21.6246517902563 26.540834995121116 27.30318522485221 24.531327989770375 100 22.41816070664955 25.818220658389553 26.38345531049527 25.38016332446563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18111.0 1 10606.0 2 2816.5 3 1974.5 4 1021.5 5 506.0 6 378.0 7 378.5 8 373.5 9 345.5 10 322.5 11 321.5 12 334.0 13 316.0 14 325.0 15 430.0 16 502.5 17 578.5 18 674.0 19 818.5 20 1092.0 21 1421.0 22 1908.0 23 2640.0 24 3697.0 25 5260.5 26 7742.0 27 10468.0 28 15170.0 29 25825.5 30 40303.5 31 54106.5 32 72462.0 33 89193.0 34 102352.0 35 117905.0 36 131925.0 37 156572.0 38 167368.0 39 156051.5 40 144726.5 41 133041.5 42 129473.5 43 138326.0 44 154299.0 45 184737.0 46 215169.0 47 235329.5 48 247470.5 49 242722.0 50 232443.5 51 239517.5 52 244744.5 53 237020.5 54 225037.5 55 196669.0 56 174377.5 57 147270.5 58 119162.0 59 112475.5 60 93553.0 61 65902.5 62 49872.5 63 41337.0 64 30678.0 65 16386.5 66 9409.0 67 6454.5 68 4664.5 69 3443.0 70 2505.0 71 1820.5 72 1426.0 73 1200.0 74 973.5 75 772.0 76 556.5 77 352.5 78 209.0 79 121.0 80 81.5 81 64.0 82 46.5 83 36.5 84 28.0 85 22.5 86 19.0 87 18.0 88 16.5 89 14.5 90 10.5 91 8.0 92 8.5 93 9.0 94 9.0 95 5.5 96 5.5 97 6.5 98 5.0 99 3.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9936902007071308 2 0.4452227401182893 3 0.47905701766842257 4 0.46495151303784105 5 0.4328401897445976 6 0.48305199951681543 7 0.4992969967960624 8 0.5013796887549402 9 0.5155230605120469 10-11 0.5300640370976665 12-13 0.5274038714592816 14-15 0.5254537144432415 16-17 0.5297137661773098 18-19 0.5284357506570893 20-21 0.5278298766326884 22-23 0.49011421861373755 24-25 0.5253685134085602 26-27 0.5049675989931888 28-29 0.5108938042943594 30-31 0.5137338387837382 32-33 0.5164224047670168 34-35 0.5290226911182275 36-37 0.5470285097808895 38-39 0.5359429084906807 40-41 0.5409319024103563 42-43 0.5382906703352339 44-45 0.548486394152104 46-47 0.5470853104706771 48-49 0.5188459008646201 50-51 0.5344755573378349 52-53 0.5263625254798427 54-55 0.536170111249831 56-57 0.52424196639444 58-59 0.5288428222672336 60-61 0.5233331553578388 62-63 0.5166401407445359 64-65 0.5291173589345401 66-67 0.5270630673205562 68-69 0.5481171896684847 70-71 0.5310391156056865 72-73 0.5347216936602478 74-75 0.5321088619300193 76-77 0.5197925790277464 78-79 0.5503134830069377 80-81 0.5244786359352215 82-83 0.524639571222953 84-85 0.5326295349197387 86-87 0.8393153926461283 88-89 1.1066099720048332 90-91 1.0966414509471136 92-93 1.0965657166940634 94-95 1.1017913801545205 96-97 1.0945776925514983 98-99 1.0902608401276426 100 1.0872409367872697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5281626.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.055145143932453 #Duplication Level Percentage of deduplicated Percentage of total 1 74.06459644152173 20.77893003193675 2 12.142952146204786 6.813445698752028 3 4.913506047018209 4.135473759440569 4 2.683751771580638 3.0117218192792263 5 1.5036145417429672 2.109206210456321 6 0.9747183903602827 1.6407519549610765 7 0.6737521238061289 1.3231549537089586 8 0.4649177103608196 1.0434667075326034 9 0.34807813305587254 0.8788844289880384 >10 1.7475292570620262 8.908700765730924 >50 0.1825351752515897 3.6078911269494904 >100 0.2181561272712343 13.687113256429095 >500 0.04347776982526053 8.546949273512865 >1k 0.03598393060162516 17.89739700603433 >5k 0.0018903378177213969 3.371163020350914 >10k+ 5.400965193489705E-4 2.2457499859369117 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 19918 0.3771187130629848 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 16451 0.31147604923180855 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 16215 0.30700772830185247 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 14703 0.2783801806489138 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 13798 0.26124530589632816 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 13678 0.2589732783048251 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 12494 0.23655593940199474 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 11311 0.214157534062427 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8489 0.16072701853557975 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 8316 0.15745151209116284 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 8168 0.15464934472830905 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 7997 0.15141170541041715 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7391 0.13993796607332665 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 7139 0.1351667081311702 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6964 0.1318533345602282 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6891 0.13047118444206388 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 6561 0.12422310856543042 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 6543 0.12388230442670496 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 6529 0.1236172345410296 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 6339 0.12001985752114973 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6219 0.11774782992964665 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6193 0.11725555728482101 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6117 0.11581660647686906 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 6046 0.11447232348522973 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5901 0.11172695681216352 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5787 0.10956853060023561 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5703 0.10797811128618345 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5623 0.1064634262251814 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 5601 0.10604688783340585 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5597 0.10597115358035575 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 5404 0.10231697587068832 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5391 0.10207083954827548 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5373 0.10173003540955002 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.8933563262525593E-5 0.0 0.0 0.0 0.0 6 1.8933563262525593E-5 0.0 0.0 0.0 0.0 7 1.8933563262525593E-5 0.0 0.0 0.0 0.0 8 1.8933563262525593E-5 0.0 0.0 0.0 0.0 9 1.8933563262525593E-5 0.0 0.0 0.0 0.0 10-11 1.8933563262525593E-5 0.0 0.0 0.0 9.466781631262797E-6 12-13 1.8933563262525593E-5 0.0 0.0 0.0 1.7040206936273035E-4 14-15 1.8933563262525593E-5 0.0 0.0 0.0 2.3666954078156992E-4 16-17 1.8933563262525593E-5 0.0 0.0 0.0 3.029370122004095E-4 18-19 1.8933563262525593E-5 0.0 0.0 1.8933563262525593E-5 3.029370122004095E-4 20-21 1.8933563262525593E-5 0.0 0.0 3.786712652505119E-5 3.6920448361924907E-4 22-23 3.786712652505119E-5 0.0 0.0 7.573425305010237E-5 3.9760482851303744E-4 24-25 3.786712652505119E-5 0.0 0.0 1.4200172446894195E-4 3.9760482851303744E-4 26-27 3.786712652505119E-5 0.0 0.0 4.260051734068258E-4 4.4493873666935147E-4 28-29 3.786712652505119E-5 0.0 0.0 0.001533618624264573 4.5440551830061424E-4 30-31 3.786712652505119E-5 0.0 0.0 0.0062196755317396575 4.5440551830061424E-4 32-33 3.786712652505119E-5 0.0 0.0 0.0175324795810987 5.301397713507166E-4 34-35 3.786712652505119E-5 0.0 0.0 0.036503909970149344 5.680068978757678E-4 36-37 3.786712652505119E-5 0.0 0.0 0.06833122981445487 5.680068978757678E-4 38-39 3.786712652505119E-5 0.0 0.0 0.12071093258023192 6.05874024400819E-4 40-41 3.786712652505119E-5 0.0 0.0 0.1790168406471795 6.05874024400819E-4 42-43 3.786712652505119E-5 0.0 0.0 0.24199933883997088 6.05874024400819E-4 44-45 3.786712652505119E-5 0.0 0.0 0.3178377264880171 6.342743692946074E-4 46-47 3.786712652505119E-5 0.0 0.0 0.414626101886048 6.626747141883957E-4 48-49 3.786712652505119E-5 0.0 0.0 0.5281044132999951 6.626747141883957E-4 50-51 3.786712652505119E-5 0.0 0.0 0.6531889989938704 6.816082774509214E-4 52-53 3.786712652505119E-5 0.0 0.0 0.7769955691675253 7.573425305010237E-4 54-55 3.786712652505119E-5 0.0 0.0 0.9168010002980143 7.573425305010237E-4 56-57 3.786712652505119E-5 0.0 0.0 1.0888218893196906 7.668093121322865E-4 58-59 3.786712652505119E-5 0.0 0.0 1.295680534744414 7.762760937635493E-4 60-61 3.786712652505119E-5 0.0 0.0 1.5151868004285045 7.952096570260749E-4 62-63 3.786712652505119E-5 0.0 0.0 1.7478992264882065 8.520103468136517E-4 64-65 3.786712652505119E-5 0.0 0.0 1.9948591589029592 8.804106917074401E-4 66-67 3.786712652505119E-5 0.0 0.0 2.2756817692127385 9.27744599863754E-4 68-69 3.786712652505119E-5 0.0 0.0 2.597239940881842 9.372113814950169E-4 70-71 3.786712652505119E-5 0.0 0.0 2.9532761312520046 9.656117263888052E-4 72-73 3.786712652505119E-5 0.0 0.0 3.3255005182116264 0.0010224124161763819 74-75 3.786712652505119E-5 0.0 0.0 3.6906248189478013 0.001069746324332696 76-77 3.786712652505119E-5 0.0 0.0 4.113165150277585 0.0010981466692264845 78-79 3.786712652505119E-5 0.0 0.0 4.570600038700203 0.0011265470141202728 80-81 3.786712652505119E-5 0.0 0.0 5.067104334915044 0.0011738809222765867 82-83 3.786712652505119E-5 0.0 0.0 5.589632813834225 0.001211748048801638 84-85 3.786712652505119E-5 0.0 0.0 6.097828585363674 0.001211748048801638 86-87 3.786712652505119E-5 0.0 0.0 6.641638389389934 0.0012590819569579521 88 3.786712652505119E-5 0.0 0.0 7.078994233972644 0.0012874823018517404 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5690 0.0 38.921 94 GTACTAG 5690 0.0 34.49329 1 TATTCTC 2785 0.0 30.86987 5 TAGGCAT 7260 0.0 30.672604 5 GCATCGT 5950 0.0 30.536673 8 CAACGCA 9720 0.0 30.353048 5 GTATCAA 11050 0.0 29.802086 1 CTGTGCG 7980 0.0 29.360722 9 TAGTACT 8060 0.0 29.203876 4 GGTATCA 7505 0.0 28.728903 1 GAACCGT 585 0.0 28.088163 6 ATCAACG 10480 0.0 28.064886 3 CTAGGCA 8250 0.0 27.734026 4 ACTGTGC 8955 0.0 27.577055 8 TCTAACG 3140 0.0 27.383718 2 GTACTGT 8870 0.0 26.675838 6 CTAACGC 3230 0.0 26.619436 3 CTAGTAC 9555 0.0 26.503813 3 TACTGTG 9800 0.0 26.301105 7 TGAACCG 645 0.0 26.221146 5 >>END_MODULE