##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5281626
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80660520074689	31.0	31.0	34.0	30.0	34.0
2	31.75910713859709	31.0	31.0	34.0	30.0	34.0
3	31.949251613044922	31.0	31.0	34.0	30.0	34.0
4	35.69973602826099	37.0	35.0	37.0	33.0	37.0
5	35.6159879552244	37.0	35.0	37.0	33.0	37.0
6	35.65446228112327	37.0	35.0	37.0	33.0	37.0
7	35.56641799324677	37.0	35.0	37.0	33.0	37.0
8	35.55153318315231	37.0	35.0	37.0	33.0	37.0
9	37.12981437913249	39.0	37.0	39.0	33.0	39.0
10-11	37.073566549392176	39.0	37.0	39.0	33.0	39.0
12-13	37.016025178609766	39.0	37.0	39.0	33.0	39.0
14-15	38.21074333926711	40.0	37.0	41.0	33.0	41.0
16-17	38.21016226821058	40.0	37.0	41.0	33.0	41.0
18-19	38.251848010442245	40.0	37.5	41.0	33.0	41.0
20-21	38.26046704177842	40.0	37.5	41.0	33.0	41.0
22-23	38.22722140871012	40.0	37.0	41.0	33.0	41.0
24-25	38.15982407690359	40.0	37.0	41.0	33.0	41.0
26-27	38.017745482167804	40.0	37.0	41.0	33.0	41.0
28-29	37.95038668016251	40.0	37.0	41.0	33.0	41.0
30-31	37.82883462403434	40.0	37.0	41.0	33.0	41.0
32-33	37.71767898749363	40.0	36.5	41.0	32.0	41.0
34-35	37.62491333161417	40.0	36.0	41.0	32.0	41.0
36-37	37.55058981836275	40.0	36.0	41.0	32.0	41.0
38-39	37.43232813152616	40.0	36.0	41.0	31.0	41.0
40-41	37.30542033835792	39.5	36.0	41.0	31.0	41.0
42-43	37.18583443810675	39.0	35.5	41.0	31.0	41.0
44-45	36.99252597590212	39.0	35.0	41.0	31.0	41.0
46-47	36.766736512581545	39.0	35.0	41.0	30.0	41.0
48-49	36.518594841815755	39.0	35.0	41.0	30.0	41.0
50-51	36.314523311571094	39.0	35.0	41.0	30.0	41.0
52-53	36.11546387419329	38.0	35.0	40.0	29.0	41.0
54-55	35.91039653318883	38.0	35.0	40.0	29.0	41.0
56-57	35.64560194152331	37.5	34.0	40.0	28.5	41.0
58-59	35.355502642557425	37.0	34.0	40.0	28.0	41.0
60-61	35.08776908853447	36.5	34.0	40.0	28.0	41.0
62-63	34.80923781805073	36.0	34.0	39.0	28.0	41.0
64-65	34.53213148753812	35.5	33.0	39.0	27.0	41.0
66-67	34.306087178456025	35.0	33.0	39.0	27.0	41.0
68-69	34.11220076923281	35.0	33.0	38.0	27.0	41.0
70-71	33.91421382354601	35.0	33.0	37.5	26.5	40.0
72-73	33.718346963605526	35.0	33.0	37.0	26.0	40.0
74-75	33.53927247025821	35.0	33.0	36.5	26.5	39.0
76-77	33.390148601964626	35.0	33.0	36.0	27.0	39.0
78-79	33.28259867321162	35.0	33.0	36.0	27.0	39.0
80-81	33.13954869201265	35.0	33.0	35.5	27.0	38.0
82-83	32.951220419620775	35.0	33.0	35.0	27.0	37.0
84-85	32.81779133925802	35.0	33.0	35.0	27.0	37.0
86-87	32.69276440626429	35.0	33.0	35.0	27.0	36.0
88-89	32.586266142282696	35.0	33.0	35.0	27.0	36.0
90-91	32.49444356718935	35.0	33.0	35.0	26.5	36.0
92-93	32.412865753841714	35.0	33.0	35.0	26.0	36.0
94-95	32.36880470143096	35.0	33.0	35.0	26.0	35.0
96-97	32.32074790982928	35.0	33.0	35.0	26.0	35.0
98-99	32.28030828006375	35.0	33.0	35.0	26.0	35.0
100	31.770296874485243	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4928724153959685
1101	2	0.6201561881240316
1101	3	0.5085766355563628
1101	4	0.3362728789559668
1101	5	0.3794203337250863
1101	6	0.3918835099812341
1101	7	0.39632324587407197
1101	8	0.42420158056449253
1101	9	0.5092764544924435
1101	10-11	0.5266955722506523
1101	12-13	0.5072418823011944
1101	14-15	0.7100071364901055
1101	16-17	0.6414116373017293
1101	18-19	0.6619659427527225
1101	20-21	0.6644535624991477
1101	22-23	0.6380314315914362
1101	24-25	0.7219555674102409
1101	26-27	0.7294933625532067
1101	28-29	0.7629934042776156
1101	30-31	0.7995451716934312
1101	32-33	0.7948440322358366
1101	34-35	0.7780487479009324
1101	36-37	0.7851932836143902
1101	38-39	0.8043402469862855
1101	40-41	0.8047208139120343
1101	42-43	0.7465200968666608
1101	44-45	0.7356341479447295
1101	46-47	0.7411188819781884
1101	48-49	0.7499070622974315
1101	50-51	0.7613514010824574
1101	52-53	0.7404264598791315
1101	54-55	0.7240957775020433
1101	56-57	0.6970626061218823
1101	58-59	0.6746874323802103
1101	60-61	0.7302391601058744
1101	62-63	0.7265890381808333
1101	64-65	0.6733765502022777
1101	66-67	0.6855946291017858
1101	68-69	0.6871473579763219
1101	70-71	0.6610309795421898
1101	72-73	0.6224681408534636
1101	74-75	0.58304793610111
1101	76-77	0.610882471651621
1101	78-79	0.5642358006747727
1101	80-81	0.5346526406016778
1101	82-83	0.5451418505100349
1101	84-85	0.5552713952721362
1101	86-87	0.5936701631676442
1101	88-89	0.5847506658602768
1101	90-91	0.5881736376616828
1101	92-93	0.5850871227524266
1101	94-95	0.5306291268180772
1101	96-97	0.5648895238113099
1101	98-99	0.5712848211696056
1101	100	0.5975449686167096
1102	1	0.45605017172843887
1102	2	0.5211462607140369
1102	3	0.43840664914292304
1102	4	0.3378952993048898
1102	5	0.3347290722714149
1102	6	0.35175256076853145
1102	7	0.33671289196507104
1102	8	0.33618359526503383
1102	9	0.46992796197784514
1102	10-11	0.4763077325096816
1102	12-13	0.5373947013099851
1102	14-15	0.6222062930330168
1102	16-17	0.6422884481512838
1102	18-19	0.6449018504915287
1102	20-21	0.602727867613936
1102	22-23	0.5673124675247365
1102	24-25	0.6309262340721133
1102	26-27	0.661132299269255
1102	28-29	0.6657918622308543
1102	30-31	0.726604953928323
1102	32-33	0.7056653023150119
1102	34-35	0.7338947081219089
1102	36-37	0.7129532783472712
1102	38-39	0.6800225362117231
1102	40-41	0.6035917239818858
1102	42-43	0.7657936800404812
1102	44-45	0.6670242146097749
1102	46-47	0.6824412393798696
1102	48-49	0.6625082229358625
1102	50-51	0.646765190018975
1102	52-53	0.5611292092723872
1102	54-55	0.6493666496804735
1102	56-57	0.6572433162750215
1102	58-59	0.6414983715204841
1102	60-61	0.5847803333141499
1102	62-63	0.6147168176495086
1102	64-65	0.5906570600232044
1102	66-67	0.547936414868019
1102	68-69	0.5412268418427146
1102	70-71	0.5179303122055856
1102	72-73	0.5366951594605069
1102	74-75	0.48003803526629696
1102	76-77	0.5011434541045361
1102	78-79	0.4745167109749886
1102	80-81	0.46080337324080034
1102	82-83	0.5365813265933852
1102	84-85	0.5478202899324174
1102	86-87	0.5193072609681906
1102	88-89	0.47689651220551355
1102	90-91	0.49249236079822367
1102	92-93	0.4930452368336944
1102	94-95	0.4569158394391124
1102	96-97	0.4984468837564222
1102	98-99	0.48616296578838103
1102	100	0.48673962186421704
1103	1	0.2790299891643393
1103	2	-0.15089500378939746
1103	3	0.19296420538031
1103	4	0.217703066663816
1103	5	0.23263664006572782
1103	6	0.279074633279528
1103	7	0.34073905643189306
1103	8	0.35165476263492934
1103	9	0.4281476774308359
1103	10-11	0.4499195549127819
1103	12-13	0.4034452632180674
1103	14-15	0.5055382289608374
1103	16-17	0.5038795239923815
1103	18-19	0.5375882203240891
1103	20-21	0.5510360753444417
1103	22-23	0.5186303216335872
1103	24-25	0.5221295915717477
1103	26-27	0.5336481277192249
1103	28-29	0.5526276351068944
1103	30-31	0.5955139654565471
1103	32-33	0.6099053831278525
1103	34-35	0.620626556831489
1103	36-37	0.6108452354854421
1103	38-39	0.6230853116957462
1103	40-41	0.6256798582214458
1103	42-43	0.6173787659217496
1103	44-45	0.601449135662655
1103	46-47	0.6066893345021498
1103	48-49	0.64526467957878
1103	50-51	0.6543578858433321
1103	52-53	0.6892723645284207
1103	54-55	0.6157451990275291
1103	56-57	0.6284940269375525
1103	58-59	0.6395386676596644
1103	60-61	0.6611431310055877
1103	62-63	0.6534306154049005
1103	64-65	0.5928227867772904
1103	66-67	0.6003258398067004
1103	68-69	0.5699781454583999
1103	70-71	0.6086551023807374
1103	72-73	0.509476217993921
1103	74-75	0.547574265836694
1103	76-77	0.534935866620657
1103	78-79	0.441037959344051
1103	80-81	0.4392473861261408
1103	82-83	0.4898136262495001
1103	84-85	0.46223228687272666
1103	86-87	0.45407599413321975
1103	88-89	0.43983026813442194
1103	90-91	0.4280561043946989
1103	92-93	0.4109151200005172
1103	94-95	0.4400873632859046
1103	96-97	0.47985182266587856
1103	98-99	0.5360053359291257
1103	100	0.580058487644294
1104	1	0.38317563466336324
1104	2	0.4485690242460585
1104	3	0.4062409036194161
1104	4	0.289788041004428
1104	5	0.2919136203953343
1104	6	0.3121714445718311
1104	7	0.2795416907232493
1104	8	0.32890850304656993
1104	9	0.3956096223363872
1104	10-11	0.39524553219364833
1104	12-13	0.4273741330116039
1104	14-15	0.5027634348326657
1104	16-17	0.5197633895003122
1104	18-19	0.5209720044597503
1104	20-21	0.5202719355577443
1104	22-23	0.49781258465043976
1104	24-25	0.5205997930494703
1104	26-27	0.5391027816701168
1104	28-29	0.5374734075466492
1104	30-31	0.5999731654044638
1104	32-33	0.6041984751652976
1104	34-35	0.601965184786657
1104	36-37	0.6230375127153138
1104	38-39	0.5848566230911132
1104	40-41	0.6146213678396393
1104	42-43	0.668038423724262
1104	44-45	0.6167932318338174
1104	46-47	0.5626832153080557
1104	48-49	0.522555308749105
1104	50-51	0.5507737089508851
1104	52-53	0.5371469210495903
1104	54-55	0.6025574677946821
1104	56-57	0.5189072895312634
1104	58-59	0.5307222543319767
1104	60-61	0.5315876405478406
1104	62-63	0.549816602016449
1104	64-65	0.46985031230106244
1104	66-67	0.41639740399856606
1104	68-69	0.4902685901849324
1104	70-71	0.4589437026693446
1104	72-73	0.4097680731729838
1104	74-75	0.40776587038920553
1104	76-77	0.3902668347335805
1104	78-79	0.3726310112378499
1104	80-81	0.40032160847855636
1104	82-83	0.3848406960885953
1104	84-85	0.3998604481850947
1104	86-87	0.38353438035852605
1104	88-89	0.3864283651084506
1104	90-91	0.386954974947713
1104	92-93	0.4018855113468476
1104	94-95	0.3966101637936674
1104	96-97	0.4173958993076994
1104	98-99	0.35490323001498325
1104	100	0.4596546642329571
1105	1	0.13692215569130184
1105	2	-0.4373272966174788
1105	3	0.005836941019509823
1105	4	0.059957936056378
1105	5	0.13852135573537794
1105	6	0.1926567620034092
1105	7	0.234520310942969
1105	8	0.23086831844918976
1105	9	0.3064436446341716
1105	10-11	0.2747750788881973
1105	12-13	0.28064876312588893
1105	14-15	0.3810778179966263
1105	16-17	0.37164029045202085
1105	18-19	0.362184823256662
1105	20-21	0.3979798178149707
1105	22-23	0.3350566499411869
1105	24-25	0.36092479594723414
1105	26-27	0.31600459541710535
1105	28-29	0.33028072481324955
1105	30-31	0.3405106664223325
1105	32-33	0.3917196115866233
1105	34-35	0.3876646965568753
1105	36-37	0.3228923859020938
1105	38-39	0.3554206402883011
1105	40-41	0.40310214752194184
1105	42-43	0.3011341936284353
1105	44-45	0.38152831804124077
1105	46-47	0.3222565453948363
1105	48-49	0.3525867849419697
1105	50-51	0.2796808667809003
1105	52-53	0.3175192456593976
1105	54-55	0.346499978320125
1105	56-57	0.3790311811207516
1105	58-59	0.37440590254659156
1105	60-61	0.4295451494115454
1105	62-63	0.3285969877357502
1105	64-65	0.3737568003966345
1105	66-67	0.39268230611521915
1105	68-69	0.3658210150082297
1105	70-71	0.3383887302027233
1105	72-73	0.2895190964078651
1105	74-75	0.2784794571924891
1105	76-77	0.263799526129759
1105	78-79	0.2456878330999004
1105	80-81	0.2681149011079498
1105	82-83	0.2267623856973131
1105	84-85	0.23865530016847458
1105	86-87	0.2497897505015274
1105	88-89	0.2605840554243102
1105	90-91	0.28616783140889623
1105	92-93	0.292616678347855
1105	94-95	0.3138419935002261
1105	96-97	0.1718087994806652
1105	98-99	0.16001917895177797
1105	100	0.19446764652464665
1106	1	0.2995230756278282
1106	2	0.3210124445952438
1106	3	0.25734231411132313
1106	4	0.18621418132374856
1106	5	0.19390708564669268
1106	6	0.18273544600997127
1106	7	0.1892701384931783
1106	8	0.22114975837463646
1106	9	0.26415878249473934
1106	10-11	0.25965594562877925
1106	12-13	0.27061161896639163
1106	14-15	0.3303565534882935
1106	16-17	0.3393598146731378
1106	18-19	0.3352268868820758
1106	20-21	0.3412347758214338
1106	22-23	0.3027985256806076
1106	24-25	0.29229498199314463
1106	26-27	0.34557059755191943
1106	28-29	0.2940033578607455
1106	30-31	0.27845590966614253
1106	32-33	0.32091502488185597
1106	34-35	0.33638246556756
1106	36-37	0.3643038000063754
1106	38-39	0.3268343341808304
1106	40-41	0.34166144793207565
1106	42-43	0.371455840970647
1106	44-45	0.3425204216595503
1106	46-47	0.35758374297546425
1106	48-49	0.3496618339454258
1106	50-51	0.32500016571470525
1106	52-53	0.32581612258934456
1106	54-55	0.3415673479947827
1106	56-57	0.3519026536947436
1106	58-59	0.35168753287633336
1106	60-61	0.27701589690361317
1106	62-63	0.29678788582441484
1106	64-65	0.3167890626647534
1106	66-67	0.3157486466207118
1106	68-69	0.24652122845024138
1106	70-71	0.28850521792178085
1106	72-73	0.38340398748768223
1106	74-75	0.2858251987385856
1106	76-77	0.30951828100562295
1106	78-79	0.2707041253913971
1106	80-81	0.2165443854987288
1106	82-83	0.2204946402240182
1106	84-85	0.25492880440554444
1106	86-87	0.28210586212880884
1106	88-89	0.2561371559030121
1106	90-91	0.24067946039421173
1106	92-93	0.3037163224157524
1106	94-95	0.25345515300825383
1106	96-97	0.26819378949343076
1106	98-99	0.24211485997222582
1106	100	0.2945539213866297
1107	1	0.11702424219618379
1107	2	-0.4777818178942539
1107	3	-0.030699349110310692
1107	4	0.07289966622131772
1107	5	0.11263149900795355
1107	6	0.15599954629466595
1107	7	0.16262527380683167
1107	8	0.1922048558166054
1107	9	0.23453911389746196
1107	10-11	0.2543592255178666
1107	12-13	0.1694601172028456
1107	14-15	0.20622400934586693
1107	16-17	0.3136291728397751
1107	18-19	0.2850447737956827
1107	20-21	0.24141219880326759
1107	22-23	0.2543166069439309
1107	24-25	0.24719775648954112
1107	26-27	0.28573071514015425
1107	28-29	0.24805668391939406
1107	30-31	0.36228804350881205
1107	32-33	0.3584810347110192
1107	34-35	0.3980655756238889
1107	36-37	0.35632615389869926
1107	38-39	0.39668689807928814
1107	40-41	0.35231788185195256
1107	42-43	0.3756159339333536
1107	44-45	0.3539026136800416
1107	46-47	0.41763703341976566
1107	48-49	0.39530186044301985
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2216	86-87	-0.7647976651703132
2216	88-89	-0.7509536274222803
2216	90-91	-0.8750259407803469
2216	92-93	-0.8122200341374111
2216	94-95	-0.8511745648263016
2216	96-97	-0.8135475660751226
2216	98-99	-0.7638098075195643
2216	100	-0.8616882186659929
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	67.0
14	261.0
15	890.0
16	2079.0
17	4445.0
18	7747.0
19	11998.0
20	17180.0
21	24604.0
22	33153.0
23	39177.0
24	42140.0
25	46018.0
26	53555.0
27	66425.0
28	84694.0
29	105806.0
30	130702.0
31	161346.0
32	199621.0
33	252186.0
34	343084.0
35	467213.0
36	699727.0
37	1169840.0
38	957122.0
39	359863.0
40	675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76685858416581	21.979751615013686	13.698440516463531	24.554949284356972
2	15.01503190232047	20.23067977371297	40.09000207140712	24.664286252559435
3	17.973806832489828	26.690883134243297	29.659113931001052	25.67619610226582
4	12.417756632166164	17.255762220198314	38.894150671573286	31.432330476062237
5	12.801262220280007	38.67164682976594	35.081805559684234	13.445285390269824
6	29.601963486244575	38.90137241826665	17.797019327002708	13.699644768486069
7	26.003004768436867	32.258413872298284	24.068876790293857	17.669704568970985
8	26.168304987895773	35.1074460781585	19.650047163506088	19.074201770439632
9	26.10889146637797	14.44888373391073	21.666963923609888	37.77526087610141
10-11	25.72924512261944	25.853174760954296	27.40604881905686	21.01153129736941
12-13	25.994475943582522	23.593813723273854	27.868349633237948	22.543360699905673
14-15	23.365066338808596	24.623116910019736	25.41818093683058	26.593635814341088
16-17	21.9295951095392	27.07978495741381	26.853843881021284	24.13677605202571
18-19	21.37259434878577	27.237824109469315	29.258158377742006	22.131423164002904
20-21	22.96113117491241	26.367687889907337	28.353772282329476	22.317408652850776
22-23	23.44996873098938	25.920192169512685	28.10734728027769	22.522491819220246
24-25	22.852015648211367	26.066111080186293	28.892021131371287	22.18985214023106
26-27	22.782926714050493	26.2260278310469	27.904442945956255	23.086602508946356
28-29	21.946849322538174	26.880916975189077	28.43288222225504	22.739351480017707
30-31	22.87183908894723	26.4095943181134	28.246036353198807	22.472530239740564
32-33	23.366355361019504	26.709454626283645	27.369573309431605	22.554616703265246
34-35	23.195287491316556	26.499256002391697	28.285408804864737	22.02004770142701
36-37	22.93165968207518	26.043466538524314	27.99889655193306	23.02597722746745
38-39	23.111500132724277	25.97307155031424	28.67095284671804	22.244475470243444
40-41	22.580882137153385	25.28460572117756	29.164846670661664	22.969665471007392
42-43	22.809213021951063	25.534803490502245	28.23662270610012	23.41936078144657
44-45	22.924133590678323	26.489910493473033	27.931909605110246	22.654046310738398
46-47	23.04089687531832	26.273499865382366	27.248843443288106	23.436759816011204
48-49	23.871665843841903	25.941842461639247	27.291944064325175	22.894547630193678
50-51	23.265337536711	25.976680853271404	27.053470565075088	23.704511044942507
52-53	22.571637976638257	26.653818350636715	26.882308592088876	23.892235080636155
54-55	22.693086774606638	26.593650400252123	26.719738882577154	23.99352394256409
56-57	23.808197557492754	25.947085952004894	26.540663076607906	23.704053413894442
58-59	22.58656679822365	26.319440171235502	27.570601037750762	23.523391992790085
60-61	22.794912509349636	27.05550748004188	26.656633762945713	23.49294624766277
62-63	23.269216714701116	26.198371486356663	26.213897008231935	24.318514790710285
64-65	22.354602540959924	26.38493335196396	26.729363268054197	24.53110083902192
66-67	23.062907142611007	25.98640077885106	26.79403085337735	24.156661225160587
68-69	22.48684123455675	25.907632704039557	27.17934138910402	24.426184672299666
70-71	22.527000173148732	25.5493874804474	27.27216528594202	24.65144706046185
72-73	23.133803870247533	25.66006188245817	27.585993404303906	23.6201408429904
74-75	23.19961860165173	26.18482180741239	27.072892638750595	23.542666952185286
76-77	22.912664080397146	26.11993681317797	26.56647822836057	24.400920878064316
78-79	22.65719794058521	26.237326869379956	26.749491986089435	24.355983203945396
80-81	22.294282418662704	26.66555144558403	27.35085385415862	23.68931228159465
82-83	23.251422171773925	25.503371652370006	28.354910078225366	22.890296097630706
84-85	23.560649481200073	26.094493747116015	26.766219373441086	23.578637398242826
86-87	22.032467260736535	26.798290236096094	27.355039018616424	23.814203484550944
88-89	22.99942522951766	25.591809572697766	27.13232890353276	24.276436294251813
90-91	22.61380925015834	25.58938446201372	27.397968119682314	24.398838168145623
92-93	23.118482919648212	25.87368652460662	26.975203697740866	24.0326268580043
94-95	21.819146584544193	25.872129212251917	27.731169445289893	24.577554757914
96-97	22.219301055056782	25.772410193596386	27.28558635635477	24.722702394992062
98-99	21.615925076284682	26.54320753902466	27.162223006506448	24.67864437818421
100	22.470081623565193	25.7654187056767	26.270376283214347	25.49412338754376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	95.0
2	74.0
3	98.5
4	77.5
5	60.0
6	125.0
7	336.0
8	591.5
9	664.0
10	570.0
11	455.0
12	447.5
13	562.5
14	724.0
15	1006.0
16	1352.5
17	1817.0
18	2511.5
19	3240.0
20	4125.0
21	5093.0
22	6168.5
23	7466.5
24	8643.0
25	9878.5
26	12068.0
27	14490.0
28	18788.0
29	28863.0
30	42726.5
31	55519.5
32	73318.0
33	89892.0
34	101308.0
35	116083.0
36	129716.5
37	154626.0
38	166394.5
39	154279.0
40	141974.5
41	130234.5
42	127126.5
43	135837.5
44	151160.5
45	183778.0
46	216120.5
47	236870.5
48	250720.5
49	243715.5
50	230436.0
51	236781.5
52	242042.0
53	235459.5
54	227723.0
55	198576.5
56	175063.5
57	148761.5
58	119686.0
59	115812.0
60	95893.5
61	65115.5
62	47747.0
63	39271.0
64	28671.5
65	14075.5
66	7791.5
67	5444.0
68	4039.0
69	2987.0
70	2145.5
71	1567.5
72	1224.0
73	1051.0
74	854.5
75	615.5
76	441.0
77	265.5
78	120.5
79	48.0
80	21.5
81	10.0
82	6.5
83	4.5
84	3.0
85	4.0
86	3.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013461763479655697
2	0.003635244146404914
3	0.001817622073202457
4	0.026753124889948663
5	0.0026317652934910573
6	0.0
7	3.976048285130375E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009069176802749759
16-17	0.0030388369036353576
18-19	0.0
20-21	4.733390815631398E-5
22-23	7.384089672384981E-4
24-25	0.0
26-27	1.2306816120641635E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.354719550380886E-4
36-37	0.0
38-39	0.0
40-41	3.976048285130375E-4
42-43	6.910750590821842E-4
44-45	0.0
46-47	0.0
48-49	4.449387366693514E-4
50-51	9.466781631262797E-6
52-53	0.0
54-55	3.3133735709419787E-4
56-57	7.573425305010237E-5
58-59	2.0826919588778152E-4
60-61	8.520103468136517E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013348162100080543
70-71	7.47875748869761E-4
72-73	0.0
74-75	1.7040206936273035E-4
76-77	0.0027453666730662112
78-79	5.396065529819794E-4
80-81	0.003067237248529146
82-83	0.0017229542568898291
84-85	0.005736869668545255
86-87	0.00812249863962348
88-89	0.007336755764228668
90-91	0.0058883381746454595
92-93	0.008624238066080408
94-95	0.0015809525324208872
96-97	0.0047617911605251865
98-99	0.0017797549466774057
100	0.08237993375524887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5281626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.265405393438044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.49839965898767	13.070862163173711
2	15.953713379578888	6.466169383357653
3	6.9144060964007465	4.2036972779526165
4	3.7071715570318085	3.0050933786509004
5	2.147443168559825	2.175940318511698
6	1.3900317815526908	1.6901734537756914
7	0.9733165326384253	1.3807257877037211
8	0.6501631718929519	1.054065620023537
9	0.4953237376081286	0.9034142709259494
>10	2.545219271993288	9.411903260897535
>50	0.26771256247918146	3.846427102157609
>100	0.32575778454661547	14.74227302276821
>500	0.06856650910389998	9.725049735455514
>1k	0.058384289086714274	21.23335244470313
>5k	0.0037365945015497134	4.919819238342603
>10k+	6.539040377711999E-4	2.1710335415998983
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	23030	0.43603996193596445	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17253	0.32666076696835405	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16606	0.31441075153750003	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15299	0.28966458435337905	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14922	0.2825266310034069	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	14865	0.28144741789744293	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	12707	0.24058878837691272	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9040	0.17115941189323136	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8171	0.15470614541809663	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	8107	0.153494397369295	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8045	0.15232051644701838	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8034	0.15211224725113062	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7790	0.14749245781507436	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7645	0.14474709114200815	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7365	0.139445693428501	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7302	0.13825287894296187	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7300	0.13821501181643683	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7189	0.1361133862942965	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7126	0.13492057180875738	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7058	0.13363308950690564	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7041	0.1333112189314427	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6990	0.1323456072050539	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6898	0.13060371938490156	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6872	0.13011144674007588	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6825	0.12922156926673717	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6802	0.1287860973116991	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6513	0.12331429752882919	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6439	0.1219132138474023	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6345	0.12013345890072488	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6189	0.11717982303177091	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5970	0.11303337267727778	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5785	0.10953066347371057	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5772	0.10928452715129773	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5763	0.10911412508193499	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5671	0.10737223726178263	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5663	0.10722076875568243	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5561	0.10528954530290482	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5538	0.10485407334786673	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5512	0.10436180070304106	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5472	0.10360445817254006	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5410	0.10243057725026347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8933563262525593E-5
2	0.0	0.0	0.0	0.0	1.8933563262525593E-5
3	0.0	0.0	0.0	0.0	1.8933563262525593E-5
4	0.0	0.0	0.0	0.0	1.8933563262525593E-5
5	0.0	0.0	0.0	0.0	1.8933563262525593E-5
6	0.0	0.0	0.0	0.0	3.786712652505119E-5
7	0.0	0.0	0.0	0.0	3.786712652505119E-5
8	0.0	0.0	0.0	0.0	3.786712652505119E-5
9	0.0	0.0	0.0	0.0	3.786712652505119E-5
10-11	0.0	0.0	0.0	0.0	3.786712652505119E-5
12-13	0.0	0.0	0.0	0.0	1.7986885099399312E-4
14-15	0.0	0.0	0.0	0.0	2.0826919588778152E-4
16-17	0.0	0.0	0.0	0.0	2.2720275915030712E-4
18-19	0.0	0.0	0.0	1.8933563262525593E-5	2.650698856753583E-4
20-21	0.0	0.0	0.0	4.733390815631399E-5	2.650698856753583E-4
22-23	0.0	0.0	0.0	9.466781631262796E-5	2.650698856753583E-4
24-25	0.0	0.0	0.0	1.4200172446894195E-4	2.650698856753583E-4
26-27	0.0	0.0	0.0	4.260051734068258E-4	2.840034489378839E-4
28-29	0.0	0.0	0.0	0.0015809525324208872	2.840034489378839E-4
30-31	0.0	0.0	0.0	0.006304876566421022	2.840034489378839E-4
32-33	0.0	0.0	0.0	0.01777861590351153	3.029370122004095E-4
34-35	0.0	0.0	0.0	0.03690151479866238	3.597377019879863E-4
36-37	0.0	0.0	0.0	0.06928737475921241	3.597377019879863E-4
38-39	0.0	0.0	0.0	0.1228504252288973	3.6920448361924907E-4
40-41	0.0	0.0	0.0	0.18233968099975273	3.7867126525051184E-4
42-43	0.0	0.0	0.0	0.2464203258617706	3.7867126525051184E-4
44-45	0.0	0.0	0.0	0.3237071310994001	3.9760482851303744E-4
46-47	0.0	0.0	0.0	0.42246459707673356	4.1653839177556304E-4
48-49	0.0	0.0	0.0	0.5383853381515465	4.63872299931877E-4
50-51	0.0	0.0	0.0	0.666035421667494	4.7333908156313984E-4
52-53	0.0	0.0	0.0	0.7923128218469085	5.490733346132422E-4
54-55	0.0	0.0	0.0	0.9341914781546441	6.05874024400819E-4
56-57	0.0	0.0	0.0	1.1087778649983926	6.342743692946074E-4
58-59	0.0	0.0	0.0	1.3189309504307953	6.532079325571329E-4
60-61	0.0	0.0	0.0	1.5418452575021404	6.626747141883957E-4
62-63	0.0	0.0	0.0	1.7776817215001592	7.384089672384981E-4
64-65	0.0	0.0	0.0	2.028712370016355	7.384089672384981E-4
66-67	0.0	0.0	0.0	2.3138518327499904	7.384089672384981E-4
68-69	0.0	0.0	0.0	2.6391115160369174	7.384089672384981E-4
70-71	0.0	0.0	0.0	3.0004491041205874	7.384089672384981E-4
72-73	0.0	0.0	0.0	3.3784292943120167	8.141432202886005E-4
74-75	0.0	0.0	0.0	3.7503223439145446	9.088110366012285E-4
76-77	0.0	0.0	0.0	4.18216852158786	9.088110366012285E-4
78-79	0.0	0.0	0.0	4.651806091533175	9.088110366012285E-4
80-81	0.0	0.0	0.0	5.161213611111426	9.182778182324912E-4
82-83	0.0	0.0	0.0	5.6972985213265765	0.001022412416176382
84-85	0.0	0.0	0.0	6.2189939234622065	0.001022412416176382
86-87	0.0	0.0	0.0	6.77617082315181	0.001069746324332696
88	0.0	0.0	0.0	7.224233597759478	0.0010981466692264845
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10120	0.0	40.886475	1
GGGCACC	5860	0.0	38.691185	94
GTATCAA	16485	0.0	36.13809	1
GTACTAG	5830	0.0	32.502293	1
TATTCTC	3010	0.0	32.00896	5
CAACGCA	18345	0.0	30.871202	5
ATCAACG	18695	0.0	30.301277	3
TAGGCAT	7345	0.0	30.073935	5
CTGTGCG	8015	0.0	29.674303	9
GTGGTAT	2480	0.0	29.19756	1
TAGTACT	8135	0.0	29.009027	4
TACCCCG	385	0.0	28.077042	5
TATCAAC	21280	0.0	27.902784	2
ACTGTGC	9095	0.0	27.74875	8
AACGCAG	21700	0.0	27.20206	6
GTACTGT	8850	0.0	27.189335	6
CTAACGC	2740	0.0	26.93695	3
CTAGGCA	8490	0.0	26.688633	4
TCTAACG	2760	0.0	26.572433	2
TACTGTG	9895	0.0	26.26523	7
>>END_MODULE