##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146454_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3606452 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12844424381636 31.0 30.0 34.0 26.0 34.0 2 30.23601007305795 31.0 30.0 34.0 26.0 34.0 3 30.445785497769 31.0 30.0 34.0 26.0 34.0 4 33.88365296418752 35.0 35.0 37.0 28.0 37.0 5 34.13613795497625 37.0 35.0 37.0 30.0 37.0 6 34.256373577133424 37.0 35.0 37.0 30.0 37.0 7 34.152356665221106 37.0 35.0 37.0 29.0 37.0 8 34.077119562384304 37.0 35.0 37.0 29.0 37.0 9 35.43453704638243 39.0 35.0 39.0 28.0 39.0 10-11 35.354598785731795 39.0 35.0 39.0 28.5 39.0 12-13 35.333331762075304 39.0 35.0 39.0 28.0 39.0 14-15 36.31215804341774 39.0 36.0 41.0 27.0 41.0 16-17 36.132436671831485 39.0 35.5 41.0 27.0 41.0 18-19 36.08565121620917 39.0 35.0 41.0 27.0 41.0 20-21 36.406492308784365 39.0 35.5 41.0 27.5 41.0 22-23 36.13754321421719 39.0 35.0 41.0 27.0 41.0 24-25 36.23360105721635 39.0 35.0 41.0 27.0 41.0 26-27 36.2983935457896 39.0 35.0 41.0 27.5 41.0 28-29 36.13772996285546 39.0 35.0 41.0 27.5 41.0 30-31 36.07101189756581 39.0 35.0 41.0 27.0 41.0 32-33 36.160931852136116 39.0 35.0 41.0 27.5 41.0 34-35 36.19439285480578 39.0 35.0 41.0 28.0 41.0 36-37 36.11186811858303 39.0 35.0 41.0 28.0 41.0 38-39 35.95654940090704 39.0 35.0 41.0 27.0 41.0 40-41 35.91314621683583 39.0 34.5 41.0 27.0 41.0 42-43 35.75045224503196 39.0 34.0 41.0 27.0 41.0 44-45 35.587413474517334 38.0 34.0 40.5 26.5 41.0 46-47 35.46077543802053 38.0 34.0 40.0 26.0 41.0 48-49 35.33573814374904 38.0 33.0 40.0 26.0 41.0 50-51 35.13595065177632 38.0 33.0 40.0 26.0 41.0 52-53 34.95826050090227 38.0 33.0 40.0 26.0 41.0 54-55 34.76630217177436 37.0 33.0 40.0 26.0 41.0 56-57 34.62230067113052 37.0 33.0 40.0 26.0 41.0 58-59 34.379588165876044 36.5 33.0 40.0 25.0 41.0 60-61 34.12414472728321 36.0 32.0 39.5 25.0 41.0 62-63 33.85607558342659 35.5 32.0 39.0 25.0 41.0 64-65 33.57033103449041 35.0 32.0 39.0 24.5 41.0 66-67 33.28053153071218 35.0 31.0 38.5 24.0 40.0 68-69 32.98722276076321 35.0 31.0 38.0 23.0 40.0 70-71 32.89726176863022 35.0 31.0 37.0 24.0 40.0 72-73 32.68030629549485 35.0 31.0 37.0 24.0 40.0 74-75 32.415370563645375 35.0 31.0 36.5 23.5 39.0 76-77 32.181006290947444 35.0 31.0 36.0 23.0 39.0 78-79 31.965249502835473 35.0 31.0 36.0 23.0 38.5 80-81 31.736218172320054 34.5 30.5 35.0 23.0 37.0 82-83 31.515373696918743 34.0 30.0 35.0 23.0 37.0 84-85 31.332236225520262 34.0 30.0 35.0 22.5 36.5 86-87 31.17959701113449 34.0 30.0 35.0 22.0 36.0 88-89 31.17871054432445 34.0 31.0 35.0 22.0 36.0 90-91 31.108815395297093 34.0 30.5 35.0 21.5 36.0 92-93 30.979391934233426 34.0 30.0 35.0 20.5 35.5 94-95 30.944893346701967 34.0 30.0 35.0 20.0 35.0 96-97 30.862059858276222 34.0 30.0 35.0 20.0 35.0 98-99 30.78958987947157 34.0 30.0 35.0 20.0 35.0 100 30.282671722790155 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0217285723216705 1101 2 1.2236412432715191 1101 3 1.1507063607225234 1101 4 1.1913965047856223 1101 5 1.1465694249805694 1101 6 0.8647668095504457 1101 7 0.8988866017680408 1101 8 0.9107357326263639 1101 9 1.170086544939565 1101 10-11 1.1911045024853664 1101 12-13 1.0489015645045257 1101 14-15 1.35420785923165 1101 16-17 1.4574928181312146 1101 18-19 1.5754854748610256 1101 20-21 1.2191357928358215 1101 22-23 1.5170307168786081 1101 24-25 1.4313576234747103 1101 26-27 1.2815395522823323 1101 28-29 1.5023419387882484 1101 30-31 1.566158732930056 1101 32-33 1.3646359709134543 1101 34-35 1.2916584466556316 1101 36-37 1.1906495637918724 1101 38-39 1.311651796086288 1101 40-41 1.2674785056825257 1101 42-43 1.3223537131098553 1101 44-45 1.3638523974238197 1101 46-47 1.3133438700421394 1101 48-49 1.3010509861958965 1101 50-51 1.328167135732059 1101 52-53 1.4118693185833848 1101 54-55 1.3910133809298273 1101 56-57 1.2780944633156963 1101 58-59 1.2265812900812278 1101 60-61 1.2320479451713382 1101 62-63 1.1702642568679096 1101 64-65 1.1293421247677031 1101 66-67 1.1299885930721345 1101 68-69 1.135222902763445 1101 70-71 1.107033753789672 1101 72-73 1.1197759557432718 1101 74-75 1.0905648716041654 1101 76-77 1.0908775246580404 1101 78-79 1.1088256748184477 1101 80-81 1.1438744511479193 1101 82-83 1.1136368811551698 1101 84-85 1.0752433494411733 1101 86-87 1.1522275716606813 1101 88-89 1.0920717227775576 1101 90-91 1.1071211812788135 1101 92-93 1.1531758805304122 1101 94-95 1.149645285138142 1101 96-97 1.1157779447696932 1101 98-99 1.0884273230550718 1101 100 1.186380933148719 1102 1 0.8590499596164491 1102 2 1.0546392013252301 1102 3 1.072241560298007 1102 4 1.1396354317002206 1102 5 0.9271977362318538 1102 6 0.7412139305136876 1102 7 0.8154585433218386 1102 8 0.841844427293573 1102 9 1.0374654738047298 1102 10-11 1.0227691456362464 1102 12-13 0.9337993404802134 1102 14-15 1.2139699982145444 1102 16-17 1.360041526498435 1102 18-19 1.3944513085456904 1102 20-21 1.146199951725201 1102 22-23 1.4533563961770852 1102 24-25 1.3037561001935387 1102 26-27 1.1295341982208242 1102 28-29 1.3381047581639365 1102 30-31 1.36238758296642 1102 32-33 1.152412937237088 1102 34-35 1.041226827282408 1102 36-37 1.0838675605650039 1102 38-39 1.158261044052594 1102 40-41 1.0540294594995245 1102 42-43 1.0686490665493409 1102 44-45 1.1274324921719696 1102 46-47 1.0624971803527075 1102 48-49 1.0986176220682609 1102 50-51 1.2423402319569519 1102 52-53 1.1702998152317292 1102 54-55 1.1404513273416015 1102 56-57 0.9897229596399626 1102 58-59 1.0269738598234142 1102 60-61 1.0449870423377305 1102 62-63 0.9697377971911649 1102 64-65 0.9027305499502205 1102 66-67 1.0625624744365183 1102 68-69 1.1303430811707216 1102 70-71 0.967922265365182 1102 72-73 0.861182925084897 1102 74-75 0.888907316518079 1102 76-77 0.9326811722084329 1102 78-79 0.8936842475755498 1102 80-81 0.9474694362076939 1102 82-83 0.9696669965992406 1102 84-85 0.9107674169920443 1102 86-87 0.8996456115919109 1102 88-89 0.8360431432088475 1102 90-91 0.8656603411089172 1102 92-93 0.9898110387140591 1102 94-95 0.9567047442489134 1102 96-97 0.9417199163268144 1102 98-99 0.9881406135221198 1102 100 0.9782530039464277 1103 1 0.711516117941617 1103 2 0.8783285315637137 1103 3 0.775497384903872 1103 4 0.809609732088532 1103 5 0.8019712720156491 1103 6 0.6513996016062791 1103 7 0.6581226010180004 1103 8 0.6761273625362989 1103 9 0.8053359398850333 1103 10-11 0.9111755770728323 1103 12-13 0.7308216514161359 1103 14-15 0.9383746923708642 1103 16-17 1.159449146355442 1103 18-19 1.2023954460970216 1103 20-21 0.9140001180473334 1103 22-23 1.127033667766277 1103 24-25 1.0102044568808353 1103 26-27 0.8926477719683632 1103 28-29 1.0936862512756846 1103 30-31 1.1868286457093333 1103 32-33 0.9989003385839794 1103 34-35 0.9309696765194317 1103 36-37 0.9491367429543303 1103 38-39 1.0129661326206616 1103 40-41 0.8300401158458897 1103 42-43 0.8596359659920836 1103 44-45 1.0201367872825173 1103 46-47 0.880028947415461 1103 48-49 0.9334296181741735 1103 50-51 0.9938224799503175 1103 52-53 1.0272534793567871 1103 54-55 0.901329395534745 1103 56-57 0.8151749609984975 1103 58-59 0.8210651591293612 1103 60-61 0.8216431588233704 1103 62-63 0.7913042085098496 1103 64-65 0.86677423698913 1103 66-67 0.8865632239081904 1103 68-69 0.8878079389169216 1103 70-71 0.7601633900842586 1103 72-73 0.6807099477768261 1103 74-75 0.739040820507924 1103 76-77 0.7817768618045093 1103 78-79 0.7154688623871905 1103 80-81 0.7836593209514113 1103 82-83 0.622196361831616 1103 84-85 0.6168040217751241 1103 86-87 0.6383994696506647 1103 88-89 0.7120309986212838 1103 90-91 0.6968324150644207 1103 92-93 0.7193084052088636 1103 94-95 0.7692370413357175 1103 96-97 0.7613971838491942 1103 98-99 0.7796918240398085 1103 100 0.798201423856689 1104 1 0.585579890840279 1104 2 0.7256957426990667 1104 3 0.747020116233351 1104 4 0.8041209460633283 1104 5 0.8186120803218202 1104 6 0.4010216668471358 1104 7 0.5407804446621398 1104 8 0.5349500030093424 1104 9 0.7366806347456958 1104 10-11 0.6995546006317355 1104 12-13 0.5991839464391475 1104 14-15 0.8373960518365493 1104 16-17 1.0565368460144526 1104 18-19 0.9647237920133307 1104 20-21 0.8196316827455874 1104 22-23 1.0256981688201492 1104 24-25 0.8960936495057155 1104 26-27 0.7327241558016695 1104 28-29 0.9770449921095121 1104 30-31 0.9869511329660199 1104 32-33 0.8353295229804019 1104 34-35 0.758012770370776 1104 36-37 0.7996499520118121 1104 38-39 0.9180967538264468 1104 40-41 0.7643645607910443 1104 42-43 0.7460524747038377 1104 44-45 0.8050840056989799 1104 46-47 0.738420201774268 1104 48-49 0.7344851683640741 1104 50-51 0.83616485630413 1104 52-53 0.7998350201051352 1104 54-55 0.7355256685846925 1104 56-57 0.6172375314589473 1104 58-59 0.6178157567361495 1104 60-61 0.6093824228959832 1104 62-63 0.6306680705701524 1104 64-65 0.5941070464195093 1104 66-67 0.7477558398859543 1104 68-69 0.6476673917293141 1104 70-71 0.5559715408944399 1104 72-73 0.550676638999974 1104 74-75 0.6016860476767221 1104 76-77 0.6237665488435695 1104 78-79 0.5880048988670836 1104 80-81 0.6306582256331943 1104 82-83 0.6274225219025631 1104 84-85 0.6528900012130876 1104 86-87 0.5926860401823895 1104 88-89 0.5731954765889462 1104 90-91 0.6210246912132433 1104 92-93 0.5949965099248473 1104 94-95 0.5373950750472254 1104 96-97 0.5912121976178035 1104 98-99 0.579133431204724 1104 100 0.6755884967333756 1105 1 0.6230054412020607 1105 2 0.775814369124479 1105 3 0.8325031231448925 1105 4 0.9782541338784156 1105 5 0.826161549531534 1105 6 0.4961009652656543 1105 7 0.5738891269212019 1105 8 0.6700716666984334 1105 9 0.6337444459781665 1105 10-11 0.765876173877146 1105 12-13 0.8004951964400959 1105 14-15 0.9397803577648887 1105 16-17 1.1075810260918786 1105 18-19 1.0717717351477276 1105 20-21 0.8733355721367602 1105 22-23 1.0169633118530967 1105 24-25 0.9334420816336362 1105 26-27 0.7428250262897436 1105 28-29 1.0451088522100491 1105 30-31 1.0659665643798988 1105 32-33 0.8553544273559481 1105 34-35 0.7949013653081138 1105 36-37 0.6845203173259691 1105 38-39 0.8405673350878402 1105 40-41 0.7147423551842067 1105 42-43 0.8164873665257915 1105 44-45 0.8868944910171521 1105 46-47 0.7364045557939605 1105 48-49 0.78607212919853 1105 50-51 0.7805377576336738 1105 52-53 0.8215033694658658 1105 54-55 0.7501489659020066 1105 56-57 0.6515334758300426 1105 58-59 0.680990221024004 1105 60-61 0.7382722482730131 1105 62-63 0.7503748389701386 1105 64-65 0.6579650029122632 1105 66-67 0.7362942013730844 1105 68-69 0.622170490860185 1105 70-71 0.6248005827016598 1105 72-73 0.6952724914494794 1105 74-75 0.5967522067339743 1105 76-77 0.5346379759864064 1105 78-79 0.6119598800496462 1105 80-81 0.6898021126424503 1105 82-83 0.6487070330875859 1105 84-85 0.5729120279822126 1105 86-87 0.6278473970404335 1105 88-89 0.6247709485730368 1105 90-91 0.7147083774880159 1105 92-93 0.71406613257383 1105 94-95 0.6252389598018695 1105 96-97 0.6212685404502771 1105 98-99 0.6629674105206966 1105 100 0.7317538992848931 1106 1 0.5609331802008484 1106 2 0.6026421317181239 1106 3 0.5585059922443136 1106 4 0.7082171178032155 1106 5 0.6984297487089606 1106 6 0.36441206475963384 1106 7 0.44450661812137326 1106 8 0.4300575974979637 1106 9 0.6286285998394376 1106 10-11 0.4654265997686835 1106 12-13 0.4540361599391147 1106 14-15 0.704604385310347 1106 16-17 0.7418976090874381 1106 18-19 0.7055072317668234 1106 20-21 0.571814430290452 1106 22-23 0.8898661847022566 1106 24-25 0.6729697262091463 1106 26-27 0.549421666193652 1106 28-29 0.7789723333810912 1106 30-31 0.782412406619784 1106 32-33 0.6490743691319167 1106 34-35 0.5877690498062904 1106 36-37 0.5105297978093475 1106 38-39 0.6347135219968152 1106 40-41 0.5850701049497928 1106 42-43 0.5651606629596415 1106 44-45 0.5434484447343877 1106 46-47 0.5152980167953487 1106 48-49 0.5709353930600685 1106 50-51 0.6186555410285095 1106 52-53 0.559418365378427 1106 54-55 0.5425026646369204 1106 56-57 0.48091813360375824 1106 58-59 0.4652590496534259 1106 60-61 0.4673262080205376 1106 62-63 0.4171617321371883 1106 64-65 0.3775815135882752 1106 66-67 0.50909025048459 1106 68-69 0.5454734346693471 1106 70-71 0.5122536945035634 1106 72-73 0.39437396270717073 1106 74-75 0.3665404461893118 1106 76-77 0.39440338204598646 1106 78-79 0.45785563321674516 1106 80-81 0.5058598704820447 1106 82-83 0.40818452872604283 1106 84-85 0.41746979821129315 1106 86-87 0.42192725082687943 1106 88-89 0.4534872316113159 1106 90-91 0.40448130111875 1106 92-93 0.4221554267148484 1106 94-95 0.406437702389006 1106 96-97 0.4019748281285871 1106 98-99 0.44029436823590373 1106 100 0.4577327294799929 1107 1 0.5826222966273313 1107 2 0.7058963254529118 1107 3 0.5880000299616626 1107 4 0.8064318256426048 1107 5 0.7441227436834836 1107 6 0.45773098121763667 1107 7 0.410321003769873 1107 8 0.5221945636504088 1107 9 0.5649347205909834 1107 10-11 0.6146754046855918 1107 12-13 0.5343123094952276 1107 14-15 0.7375691982120003 1107 16-17 0.7604809869263107 1107 18-19 0.8725550044471362 1107 20-21 0.6083124538068816 1107 22-23 0.8800734528211223 1107 24-25 0.5490967377134623 1107 26-27 0.5299348624499274 1107 28-29 0.7393830173363369 1107 30-31 0.7403480538032383 1107 32-33 0.6575890078053277 1107 34-35 0.5049477672588765 1107 36-37 0.6094697634890096 1107 38-39 0.5406872411128916 1107 40-41 0.43372798943384794 1107 42-43 0.594200142288642 1107 44-45 0.5242929701156811 1107 46-47 0.4999123617322212 1107 48-49 0.43927619748669144 1107 50-51 0.6260365470871037 1107 52-53 0.6430641677038125 1107 54-55 0.5716033755581407 1107 56-57 0.37650387753203773 1107 58-59 0.36790109162579654 1107 60-61 0.4918415536753358 1107 62-63 0.5871821645558981 1107 64-65 0.5823449374794336 1107 66-67 0.5302976674416371 1107 68-69 0.5150663950608774 1107 70-71 0.3692772153146393 1107 72-73 0.36902999328028585 1107 74-75 0.42935377246012507 1107 76-77 0.4518412968229839 1107 78-79 0.41578821608460004 1107 80-81 0.43018987976254763 1107 82-83 0.4858105377613988 1107 84-85 0.5111540795855092 1107 86-87 0.597958228868869 1107 88-89 0.5448971888860008 1107 90-91 0.5549694028190046 1107 92-93 0.5992776294324571 1107 94-95 0.5491351099779997 1107 96-97 0.5222998863505843 1107 98-99 0.6149936822669702 1107 100 0.6284246300560845 1108 1 0.3889066763459432 1108 2 0.5401079732804099 1108 3 0.47647172887364775 1108 4 0.6560582879713266 1108 5 0.4836076652927872 1108 6 0.3313431624687837 1108 7 0.32351773549164875 1108 8 0.3057206633074969 1108 9 0.3375298689758921 1108 10-11 0.4153651863770307 1108 12-13 0.2863339568993766 1108 14-15 0.506934946204602 1108 16-17 0.6531730829425655 1108 18-19 0.756542063984547 1108 20-21 0.36166519077821846 1108 22-23 0.6128967174302957 1108 24-25 0.45598058400842234 1108 26-27 0.432838987310582 1108 28-29 0.5888155138436773 1108 30-31 0.6804466948429919 1108 32-33 0.6244765937105257 1108 34-35 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10-11 -1.5317440326545508 2216 12-13 -1.7523884577681983 2216 14-15 -2.1890243489058463 2216 16-17 -1.816383069733142 2216 18-19 -3.631896107101163 2216 20-21 -1.9693532947667904 2216 22-23 -1.4912279439917882 2216 24-25 -1.6367383990168207 2216 26-27 -1.6362317714276955 2216 28-29 -1.5789280194529596 2216 30-31 -1.7857979229821197 2216 32-33 -1.6680700518051381 2216 34-35 -1.8978802870397615 2216 36-37 -2.0313722589386884 2216 38-39 -2.0097554108642868 2216 40-41 -2.079344998958831 2216 42-43 -1.8746174850967634 2216 44-45 -1.8872799199098935 2216 46-47 -2.122574358708455 2216 48-49 -2.1103500075103554 2216 50-51 -1.9661235945615303 2216 52-53 -2.0457072271417616 2216 54-55 -1.9019850564701883 2216 56-57 -1.984552585186826 2216 58-59 -2.0283658767000006 2216 60-61 -2.0425279131749434 2216 62-63 -1.9192362891131935 2216 64-65 -1.9720569905158492 2216 66-67 -1.88135883462407 2216 68-69 -2.0294348837115024 2216 70-71 -2.118451488206901 2216 72-73 -2.0502597962141067 2216 74-75 -2.127467986343728 2216 76-77 -1.9877613000686125 2216 78-79 -2.089484260799132 2216 80-81 -2.023341499344287 2216 82-83 -2.2109482660945936 2216 84-85 -2.0384573703456326 2216 86-87 -2.0131326623371137 2216 88-89 -1.9757353116058347 2216 90-91 -2.051758907234113 2216 92-93 -1.9024142847914263 2216 94-95 -1.912531228798315 2216 96-97 -1.8865383981270831 2216 98-99 -2.0129130657165035 2216 100 -1.9954097197373422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 14720.0 1 2426.0 2 407.0 3 374.0 4 330.0 5 211.0 6 155.0 7 235.0 8 389.0 9 774.0 10 1584.0 11 3619.0 12 6532.0 13 9438.0 14 11864.0 15 14327.0 16 16469.0 17 19050.0 18 21302.0 19 23896.0 20 26806.0 21 30027.0 22 33927.0 23 38400.0 24 42801.0 25 47922.0 26 53868.0 27 61358.0 28 71469.0 29 83809.0 30 99006.0 31 116866.0 32 142342.0 33 178240.0 34 237620.0 35 315940.0 36 460546.0 37 677297.0 38 569745.0 39 170204.0 40 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41867176746199 19.95421046661915 12.472228718019092 24.154889047899772 2 15.702565887964347 19.48476134108554 40.42616718309369 24.386505587856423 3 18.831359158002194 25.885676330346392 28.660629258286995 26.622335253364422 4 12.416896656142914 16.179373126736063 38.808276111060195 32.59545410606083 5 13.483805047341276 38.20085601116842 34.76854029459951 13.546798646890798 6 31.13105535428352 37.3926790349827 17.836448352059854 13.639817258673931 7 27.787947584916182 31.407918999227814 22.9680349136647 17.836098502191298 8 26.463758318004064 34.871982367879625 19.80241614000024 18.861843174116075 9 26.4190715139968 15.015188436108092 20.722355833884023 37.84338421601108 10-11 25.771089443852794 25.581608949333095 27.544387147326905 21.102914459487202 12-13 25.93954321240935 23.359632143717274 27.516970091607273 23.183854552266098 14-15 23.34143811064568 24.72680870421307 24.732397404835996 27.19935578030525 16-17 22.701825159753046 27.382413840395248 25.99470035780669 23.921060642045017 18-19 22.3153748086893 27.726389148089154 27.37305757182 22.58517847140155 20-21 23.78261960912427 26.879072202895127 26.49425081501549 22.84405737296511 22-23 24.037785431434948 26.50022214677235 26.298109683523396 23.163882738269308 24-25 23.512366211736282 26.670436112423886 26.84517598512774 22.972021690712097 26-27 23.35313062463954 26.8665913010545 26.087430891438263 23.692847182867695 28-29 22.793147227750875 27.328288079190116 26.54025800944198 23.338306683617027 30-31 23.40848189992061 26.96850582980988 26.411312321426404 23.211699948843105 32-33 23.611565381895208 27.217444927167637 25.65719840155524 23.513791289381906 34-35 23.72921727762191 27.01544148084345 26.430563511609336 22.82477772992531 36-37 23.50441993309392 26.619478150416846 26.233732452069646 23.642369464419588 38-39 23.594839704253808 26.6965925349803 26.66072888375954 23.047838877006356 40-41 23.30011625281223 26.116710017535492 27.11705570935124 23.466118020301032 42-43 23.2217987303421 26.300267513551308 26.553368340178217 23.924565415928374 44-45 23.34141767741878 26.9632437893948 26.522512746851763 23.172825786334663 46-47 23.43403506717631 26.757917158047633 26.149873286439558 23.6581744883365 48-49 23.83729648403117 26.483707852328532 26.29492931884201 23.384066344798292 50-51 23.429158351297506 26.434622451343415 26.369892895164604 23.766326302194475 52-53 23.19807232435214 26.858791264080356 26.15189881180187 23.791237599765633 54-55 23.058452437701934 26.750924818590594 26.263161416038738 23.927461327668734 56-57 23.8171359731454 26.411267759527806 26.037272935945076 23.734323331381713 58-59 23.108250611095983 26.54509231876952 26.673360452270956 23.673296617863542 60-61 23.03531457669628 27.096533403648536 26.202258327829504 23.665893691825676 62-63 23.362627800375826 26.571287538790944 25.93241805195464 24.133666608878592 64-65 22.6959872699618 26.728229935687775 26.284512785718878 24.29127000863155 66-67 23.28438699869171 26.37787785674176 26.189013771566028 24.148721373000505 68-69 22.852744572581372 26.39036653406892 26.452162770701808 24.304726122647896 70-71 22.76519433695684 26.11674949674057 26.6383022968533 24.479753869449286 72-73 23.182358357467642 26.251464575463373 26.824889601047275 23.74128746602171 74-75 23.263021199568982 26.494450512897743 26.590288247737476 23.652240039795796 76-77 23.172604983319346 26.314418557461362 26.2891237228199 24.223852736399394 78-79 22.973402685507796 26.373148100517437 26.39934266830219 24.254106545672578 80-81 22.712273874042626 26.623929944332435 26.832568325934126 23.83122785569081 82-83 23.270851872318453 25.866168097173663 27.608211344778784 23.2547686857291 84-85 23.377683951597437 26.277790105395553 26.572793405975837 23.771732537031173 86-87 22.44395976203958 26.767247586491344 26.944361722207077 23.844430929262 88-89 23.24982679325409 25.78520625131254 26.755402354153752 24.209564601279613 90-91 22.91628935299794 25.857541684866504 26.982459750560857 24.243709211574703 92-93 23.131315368497074 25.996693481779946 26.971883661247016 23.900107488475967 94-95 22.245603636753096 25.876356787145983 27.480838018806192 24.397201557294732 96-97 22.533505281085514 25.763525115819423 27.169716636645898 24.53325296644916 98-99 21.993437878366947 26.29591950299666 27.067027665588583 24.643614953047805 100 22.597282967330237 25.741925066591463 26.505589422062897 25.155202544015403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12297.0 1 7205.5 2 2038.5 3 1558.0 4 880.5 5 522.5 6 419.5 7 395.5 8 377.0 9 347.5 10 328.5 11 324.0 12 320.0 13 333.5 14 348.5 15 381.0 16 435.0 17 501.5 18 619.5 19 799.5 20 1049.5 21 1389.5 22 1907.5 23 2688.5 24 3587.5 25 4857.0 26 6906.5 27 9379.5 28 12753.5 29 18905.5 30 27699.0 31 36508.5 32 47875.5 33 57950.0 34 65114.0 35 73596.0 36 82607.0 37 97180.5 38 104470.5 39 100119.5 40 96108.5 41 92599.5 42 93149.5 43 99592.5 44 108845.0 45 125870.0 46 142903.5 47 153984.5 48 160656.5 49 159004.5 50 154289.0 51 157936.0 52 160026.0 53 156031.0 54 150306.5 55 134939.5 56 121448.5 57 105326.5 58 88810.5 59 82145.0 60 69420.0 61 53136.0 62 42008.0 63 33763.0 64 24909.5 65 15705.5 66 10952.0 67 8215.0 68 6008.0 69 4406.5 70 3330.5 71 2518.5 72 1847.5 73 1460.0 74 1219.5 75 943.5 76 634.0 77 392.5 78 238.5 79 141.5 80 94.0 81 59.0 82 41.0 83 37.0 84 31.5 85 23.5 86 19.5 87 14.5 88 12.5 89 11.0 90 6.5 91 6.5 92 6.5 93 6.0 94 4.5 95 3.5 96 3.5 97 2.5 98 2.0 99 1.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9917503407781388 2 0.44578438864568276 3 0.47897490386673663 4 0.4656931521617368 5 0.4328631020182717 6 0.48066631692311446 7 0.49752499132111005 8 0.49913322012881356 9 0.5136627355639283 10-11 0.5271524478906139 12-13 0.5258353639532704 14-15 0.5228407310009949 16-17 0.5279426982530199 18-19 0.5276238252997683 20-21 0.5252669382539958 22-23 0.4882499475939234 24-25 0.5222168491359375 26-27 0.5037222178473469 28-29 0.5081864391928688 30-31 0.5121515550463448 32-33 0.5154511969104261 34-35 0.5278595140043456 36-37 0.5443854514076438 38-39 0.5338903720332338 40-41 0.5390893875753788 42-43 0.5363720354520177 44-45 0.5457995836351073 46-47 0.544080442495838 48-49 0.5171287459253582 50-51 0.5329060250905877 52-53 0.5245876002231556 54-55 0.5340983326549196 56-57 0.5222723053017204 58-59 0.5280813386674771 60-61 0.5212186381518457 62-63 0.5165741842675294 64-65 0.5277070095484426 66-67 0.5254887629171274 68-69 0.5452450219772785 70-71 0.5292597821903633 72-73 0.5333496744168508 74-75 0.5306739144178267 76-77 0.5202620192920909 78-79 0.5498617477787033 80-81 0.5241994070626754 82-83 0.5227991388766577 84-85 0.5324901038472161 86-87 0.8381367615595605 88-89 1.106489147782918 90-91 1.0972002400142855 92-93 1.0968675030195882 94-95 1.1023022072663105 96-97 1.0946215283053815 98-99 1.0900741227111854 100 1.086330831520841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3606452.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.64564619166965 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85928352621033 30.074903150336883 2 12.231994402778195 9.698906446220557 3 4.553803065617921 5.41615395498086 4 2.274652535531811 3.6072027853071376 5 1.2873909051492494 2.551972216796024 6 0.7916262249566104 1.8830719938406115 7 0.5187319631664473 1.439582471600503 8 0.3681028915074831 1.1674941599068986 9 0.26607179256271324 0.9493729334572185 >10 1.5163245293284175 11.138507026151819 >50 0.14302744067457698 3.9672042276554076 >100 0.15277567950595075 12.810062275652163 >500 0.025168755760543216 6.713909826122438 >1k 0.010486981566490097 6.956826382006838 >5k 4.893924731028712E-4 1.3199094478312507 >10k+ 6.991321044326731E-5 0.3049207021333807 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11001 0.30503663988873275 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9588 0.26585685876312787 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7802 0.21633450271901583 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6845 0.18979872739190762 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6300 0.17468692221607277 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6130 0.1699731481245279 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5769 0.15996331020071805 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5186 0.14379783787500847 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 4113 0.11404560493249322 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3776 0.10470124099807789 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3732 0.10348120535085452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.545616578288024E-5 2 0.0 0.0 0.0 0.0 5.545616578288024E-5 3 0.0 0.0 0.0 0.0 5.545616578288024E-5 4 0.0 0.0 0.0 0.0 5.545616578288024E-5 5 0.0 0.0 0.0 0.0 1.386404144572006E-4 6 0.0 0.0 0.0 0.0 1.386404144572006E-4 7 0.0 0.0 0.0 0.0 1.386404144572006E-4 8 0.0 0.0 0.0 0.0 1.386404144572006E-4 9 0.0 0.0 0.0 2.772808289144012E-5 1.386404144572006E-4 10-11 0.0 0.0 0.0 2.772808289144012E-5 1.5250445590292064E-4 12-13 0.0 0.0 0.0 2.772808289144012E-5 4.159212433716018E-4 14-15 0.0 1.386404144572006E-5 0.0 5.545616578288024E-5 4.57513367708762E-4 16-17 0.0 2.772808289144012E-5 0.0 5.545616578288024E-5 5.268335749373622E-4 18-19 0.0 5.545616578288024E-5 0.0 5.545616578288024E-5 6.654739893945629E-4 20-21 0.0 5.545616578288024E-5 0.0 6.93202072286003E-5 7.209301551774431E-4 22-23 0.0 5.545616578288024E-5 0.0 9.704829012004043E-5 7.209301551774431E-4 24-25 0.0 5.545616578288024E-5 0.0 3.3273699469728145E-4 7.209301551774431E-4 26-27 0.0 5.545616578288024E-5 0.0 7.347941966231632E-4 8.179784452974835E-4 28-29 0.0 6.93202072286003E-5 0.0 0.00234302300432669 8.595705696346436E-4 30-31 0.0 8.318424867432036E-5 0.0 0.007403398132014512 9.011626939718039E-4 32-33 0.0 9.704829012004043E-5 0.0 0.020158316262076967 9.427548183089641E-4 34-35 0.0 1.1091233156576048E-4 0.0 0.040524593145839734 9.704829012004042E-4 36-37 0.0 1.1091233156576048E-4 0.0 0.0745746789365282 9.704829012004042E-4 38-39 0.0 1.1091233156576048E-4 0.0 0.13059927041868297 0.0010259390669832844 40-41 0.0 1.1091233156576048E-4 0.0 0.19437386106899524 0.0010536671498747245 42-43 0.0 1.1091233156576048E-4 0.0 0.26022805793616555 0.0010536671498747245 44-45 0.0 1.1091233156576048E-4 0.0 0.340681090445679 0.0011229873571033248 46-47 0.0 1.1091233156576048E-4 0.0 0.44427320812809934 0.001206171605777645 48-49 0.0 1.1091233156576048E-4 0.0 0.564585359794058 0.0012200356472233651 50-51 0.0 1.1091233156576048E-4 0.0 0.6978326621288735 0.0012754918130062456 52-53 0.0 1.1091233156576048E-4 0.0 0.8268514318227445 0.0014279962689091662 54-55 0.0 1.1091233156576048E-4 0.0 0.9710235988167872 0.0014418603103548862 56-57 0.0 1.1091233156576048E-4 0.0 1.149121075228507 0.0014418603103548862 58-59 0.0 1.1091233156576048E-4 0.0 1.3671053988795636 0.0014418603103548862 60-61 0.0 1.1091233156576048E-4 0.0 1.5940597573460011 0.0014418603103548862 62-63 0.0 1.1091233156576048E-4 0.0 1.8309962256533567 0.0014695883932463263 64-65 0.0 1.1091233156576048E-4 0.0 2.0792734798633115 0.0015389086004749266 66-67 0.0 1.1091233156576048E-4 0.0 2.3643043079458703 0.0015666366833663667 68-69 0.0 1.1091233156576048E-4 0.0 2.6903588346663145 0.0015805007248120867 70-71 0.0 1.386404144572006E-4 0.0 3.0528203342232203 0.0015943647662578068 72-73 0.0 1.6636849734864072E-4 0.0 3.43279211812607 0.0016914130563778472 74-75 0.0 1.6636849734864072E-4 0.0 3.8095474444135125 0.0017745973050521677 76-77 0.0 1.6636849734864072E-4 0.0 4.228601961151846 0.001857781553726488 78-79 0.0 1.6636849734864072E-4 0.0 4.6935187269926235 0.001871645595172208 80-81 0.0 1.9409658024008083E-4 0.0 5.200651499035617 0.0018855096366179281 82-83 0.0 1.9409658024008083E-4 0.0 5.72673641573491 0.001954829843846528 84-85 0.0 1.9409658024008083E-4 0.0 6.247220259690133 0.0019686938852922485 86-87 0.0 1.9409658024008083E-4 0.0 6.796319485189322 0.0020241500510751287 88 0.0 1.9409658024008083E-4 0.0 7.2375841963236995 0.002079606216858009 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 3455 0.0 35.132664 94 GTACTAG 2865 0.0 34.75023 1 GTATCAA 6945 0.0 33.154877 1 CAACGCA 6570 0.0 32.179317 5 TAGTACT 4310 0.0 31.286612 4 CTGTGCG 4160 0.0 30.808165 9 GGTATCA 4335 0.0 30.15022 1 ATCAACG 7005 0.0 30.117739 3 TAGGCAT 3740 0.0 29.771889 5 CTAGTAC 4695 0.0 28.422804 3 TCTAACG 1455 0.0 28.096914 2 CTAACGC 1475 0.0 28.03334 3 AACGCAG 8035 0.0 27.749157 6 GTACTGT 4750 0.0 27.66983 6 TATCAAC 8490 0.0 27.06472 2 GTACATG 14460 0.0 26.920649 1 GCATCGT 3380 0.0 26.803701 8 TACTGTG 5190 0.0 26.410076 7 GGCGTCG 2280 0.0 26.352951 8 CGGGCGT 2195 0.0 26.303444 6 >>END_MODULE