FastQCFastQC Report
Mon 16 Jan 2017
ERR1146454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3606452
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC109330.3031511302521148No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT78140.2166672397137131No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA77500.21489264240866093No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA68780.19071375412732514No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC68570.1901314643866049No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC67870.18819049858420409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62930.17449282563583268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56490.15663594025374525No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC56170.15574864160121915No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG42440.11767798379127185No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC40650.11271465695370408No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG39530.1096091116698628No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT38640.10714131229252462No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT38310.1062262855571071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63450.045.873261
GTATCAA108650.038.2147981
GGGCACC35250.033.49530494
GTACTAG27450.031.8618661
GTGGTAT22000.030.9917351
CAACGCA130500.030.611615
ATCAACG131550.030.3778063
TAGTACT41750.030.2908924
TAGGCAT36100.029.2922735
TATCAAC143900.028.7838862
CTGTGCG44000.028.4142919
AACGCAG147250.027.8939366
CTAGTAC47450.027.841873
GTACTGT47250.027.6500976
TCAACGC154750.026.0962894
TACTGTG55800.025.1820247
ACGCAGA157700.025.121737
CTAGGCA42300.025.0068154
CGCAGAG161300.024.5901818
TATTCTC18350.024.5874185
Produced by FastQC (version 0.11.5)